KR890011996A - 세포연관 글루코실트랜스퍼라제, 그의 항체 및 유효성분으로서 상기 항체를 함유하는 이의 카리에스 예방조성물 - Google Patents

세포연관 글루코실트랜스퍼라제, 그의 항체 및 유효성분으로서 상기 항체를 함유하는 이의 카리에스 예방조성물 Download PDF

Info

Publication number
KR890011996A
KR890011996A KR1019890000697A KR890000697A KR890011996A KR 890011996 A KR890011996 A KR 890011996A KR 1019890000697 A KR1019890000697 A KR 1019890000697A KR 890000697 A KR890000697 A KR 890000697A KR 890011996 A KR890011996 A KR 890011996A
Authority
KR
South Korea
Prior art keywords
glucosyltransferase
antibody
cell
linked
caries
Prior art date
Application number
KR1019890000697A
Other languages
English (en)
Other versions
KR940010861B1 (ko
Inventor
도시오 호리꼬시
주니찌로 히라오까
이사무 후지따
도루 도꼬로
요시까쯔 고다마
히데아끼 요꼬야마
Original Assignee
가네보가부시끼가이샤
겐 코포레이션
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 가네보가부시끼가이샤, 겐 코포레이션 filed Critical 가네보가부시끼가이샤
Publication of KR890011996A publication Critical patent/KR890011996A/ko
Application granted granted Critical
Publication of KR940010861B1 publication Critical patent/KR940010861B1/ko

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q11/00Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66Enzymes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/02Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04Antibacterial agents
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
    • C07K16/1275Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Streptococcus (G)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • C12N1/205Bacterial isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/46Streptococcus ; Enterococcus; Lactococcus
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/822Microorganisms using bacteria or actinomycetales
    • Y10S435/885Streptococcus
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827Proteins from mammals or birds
    • Y10S530/85Reproductive organs or embryos
    • Y10S530/852Sperm
    • Y10S530/853Ovary; eggs; embryos

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Dental Preparations (AREA)

Abstract

내용 없음

Description

세포연관 글루코실트랜스퍼라제, 그의 항체 및 유효성분으로서 상기 항체를 함유하는 이의 카리에스 예방조성물
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 실시예3 에서의 DEAE-세파셀로부터 용출된 부분에서의 단백질 농도(A280), CTF 활성 및 FTase(D-프록토실트랜스퍼라제)활성을 나타낸다.
제2도는 실시예3에서의 히드록실아파타이트로부터 용출된 부분에서의 단백질 농도(A280), CTF 활성 및 FTase활성을 나타낸다.
제3도는 실시예3 에서의 면역된 암탉의 항체 역가의 변화를 나타낸다.

Claims (7)

  1. a) 수크로스와 반응하고 물에 불용성인 글루칸을 합성하며; b) 6.7 과 7.0사이에서 최적 pH를 가지며; c) 15와 50℃사이에서 최적 온도를 가지며;d) 80℃에서 5분간 열처리로 그의 활성을 상실하며; e) SDS-폴리아크릴아미드겔 전기영동으로 측정하여 150 내지 165 킬로달톤의 분자량을 가지며; f) 효소 그 자체에 특이적인 항체를 생성시키는 동물에 항원성을 가지는 생리화학적 특성을 갖는 세포 연관 글루코실트랜 스퍼라제.
  2. 수크로스로부터 물에 불용성인 글루칸을 합성하는 반응을 촉매하는 세포연관 글루코실트랜스퍼라제를 생성하는 능력을 가지는 혈청 c,e 또는 f중의 하나의 에스 무탄스의 배양세포로부터 글루코실트랜스퍼라제 활성을 추출하고, 두 흡착과정, 즉 음이온 교환기를 사용하는 하나와 히드록실아파타이트를 사용하는 다른 하나로 구성된 방법에 의해 글루코실트랜스퍼라제를 정제하는 단계를 특징으로하는 제1항에 따른 세포 연관 글루코실트랜스퍼라제의 분리 및 정제방법.
  3. 스크로스로부터 물에 불용성인 글루칸을 합성하는 반응을 촉매하는 세포연관 글루코실트랜스퍼라제를 생성하는 능력을 갖는 혈청형 c,e 또는 f중의 하나의 에스 무탄스를 배양하고, 수득한 배양 세포로 부터 제1항에 따른 분리한 세포연관 글루코실트랜스퍼라제를 제조하는 단계를 특징으로하는 물에 불용성인 글루칸을 합성하는 효소 활성을 갖는 세포연관 글루코실트랜스퍼라제의 생성방법.
  4. 제1항에 따른 분리한 세포 연관 글루코실트랜스퍼라제로써 면역화한 암탉에 의해 생성된 계란으로부터 제조하며 이의 카리에스를 생성하는 혈청형 c,e 또는 f의 에스 무탄스에 대하여 면역학적 활성을 나타내는 항체.
  5. 제1항에 따른 분리한 세포 연관 글루코실트랜스퍼라제로써 암탉을 면역화하고, 면역화된 암탉의 계란으로부터 상기 글루코실트랜스퍼라제에 대하여 면역학적 활성을 갖는 임무노글로볼린을 제조하는 단계를 특징으로하는, 이의 카리에스를 생성하는 혈청형 c,e 또는 f의 이의 에스 무탄스에 대하여 면역학적 활성을 나타내는 항체의 제조방법.
  6. 유효성분으로서 제4항에 따른 항체를 함유하는 이의 카리에스 예방조성물.
  7. 제4항에 따른 항체와 적어도 담체를 혼합하는 단계를 포함하는 이의 카리에스 예방조성물의 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890000697A 1988-01-22 1989-01-23 세포연관 글루코실트랜스퍼라제, 그의 항체 및 유효성분으로서 상기 항체를 함유하는 이의 카리에스 예방 조성물 KR940010861B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP63010853A JP2641228B2 (ja) 1988-01-22 1988-01-22 抗体およびそれを有効成分とするう蝕予防剤、並びにこれらの製造方法
JP63-10853 1988-01-22
JP10853/88 1988-01-22

Publications (2)

Publication Number Publication Date
KR890011996A true KR890011996A (ko) 1989-08-23
KR940010861B1 KR940010861B1 (ko) 1994-11-18

Family

ID=11761913

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019890000697A KR940010861B1 (ko) 1988-01-22 1989-01-23 세포연관 글루코실트랜스퍼라제, 그의 항체 및 유효성분으로서 상기 항체를 함유하는 이의 카리에스 예방 조성물

Country Status (7)

Country Link
US (1) US5439680A (ko)
EP (1) EP0334467B1 (ko)
JP (1) JP2641228B2 (ko)
KR (1) KR940010861B1 (ko)
CN (1) CN1033865C (ko)
CA (1) CA1325192C (ko)
DE (1) DE68921908T2 (ko)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2689511B2 (ja) * 1988-08-18 1997-12-10 ライオン株式会社 食 品
GB9409387D0 (en) * 1994-05-11 1994-06-29 Unilever Plc Glucan-binding domains (gbp's) and hybird proteins containing gbd's as novel active systems targeted to dental plaque
RU2122520C1 (ru) * 1996-10-31 1998-11-27 Акционерное общество закрытого типа "ОСТИМ" Способ получения суспензии гидроксиапатита
US5952205A (en) 1998-02-06 1999-09-14 Neose Technologies, Inc. Process for processing sucrose into glucose and fructose
US6231857B1 (en) 1998-09-28 2001-05-15 The Regents Of The University Of California Antibodies to S. mutans and uses thereof
EP1313506A1 (en) * 2000-08-24 2003-05-28 Washington Dental Service Immunologic method for the prevention of dental caries
US7875598B2 (en) 2004-03-04 2011-01-25 The Regents Of The University Of California Compositions useful for the treatment of microbial infections
US7517541B2 (en) 2005-01-18 2009-04-14 A.M. Todd Company Oral care compositions derived from the Labiatae family
WO2008030988A2 (en) 2006-09-06 2008-03-13 The Regents Of The University Of California Selectively targeted antimicrobial peptides and the use thereof
US20080311129A1 (en) * 2006-11-02 2008-12-18 Camas Incorporated Adherence inhibitor directed to and method of making and using
JP4982636B2 (ja) * 2007-03-29 2012-07-25 株式会社ファーマフーズ 虫歯予防用組成物
JP5868103B2 (ja) 2011-09-30 2016-02-24 日本ゼトック株式会社 口腔用組成物
CN109593129B (zh) * 2018-12-19 2022-09-06 广东工业大学 一种用于预防龋齿的卵黄抗体及其制备方法和卵黄抗体制剂

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1505513A (en) * 1975-05-23 1978-03-30 Stolle Res & Dev Dental caries inhibiting product
US4150116A (en) * 1978-02-21 1979-04-17 Forsyth Dental Infirmary For Children Immunization against dental caries with glucosyltransferase antigens
JPS56145222A (en) * 1980-04-28 1981-11-11 Toshiyuki Hamaoka Improved antibody and its preparation
US4748018A (en) * 1984-02-07 1988-05-31 Stolle Research & Development Corp. Method of passive immunization of mammals using avian antibody
US4693888A (en) * 1983-08-11 1987-09-15 Lion Corporation Caries-preventive composition
JPS6038327A (ja) * 1983-08-11 1985-02-27 Lion Corp う蝕予防剤
JPH0641424B2 (ja) * 1984-11-06 1994-06-01 ライオン株式会社 う蝕予防剤
JPS62223112A (ja) * 1986-03-25 1987-10-01 Rooto Seiyaku Kk 歯周病治療剤
JPH0728339B2 (ja) * 1986-07-01 1995-03-29 富士通株式会社 代表選択方式
US5281524A (en) * 1988-01-22 1994-01-25 Kanebo, Ltd. Cell-associated glucosyltransferase from Streptococcus mutans serotype, c, e or f
US5281746A (en) * 1993-02-01 1994-01-25 Monsanto Company Process for preparing peroxyacid precursors having amide moieties in the fatty chain

Also Published As

Publication number Publication date
KR940010861B1 (ko) 1994-11-18
DE68921908D1 (de) 1995-05-04
EP0334467A2 (en) 1989-09-27
CA1325192C (en) 1993-12-14
JP2641228B2 (ja) 1997-08-13
CN1033865C (zh) 1997-01-22
DE68921908T2 (de) 1995-07-27
JPH01190635A (ja) 1989-07-31
EP0334467B1 (en) 1995-03-29
US5439680A (en) 1995-08-08
EP0334467A3 (en) 1990-06-06
CN1036036A (zh) 1989-10-04

Similar Documents

Publication Publication Date Title
Gibbons et al. Synthesis of extracellular dextran by cariogenic bacteria and its presence in human dental plaque
CA1152000A (en) Immunization against dental caries with glucosyltransferase antigens
US5538883A (en) Maltose-trehalose converting enzyme
KR890011996A (ko) 세포연관 글루코실트랜스퍼라제, 그의 항체 및 유효성분으로서 상기 항체를 함유하는 이의 카리에스 예방조성물
US5527699A (en) Maltohexaose and maltoheptaose-forming amylase from a microorganism
Koga et al. Cellular adherence, glucosyltransferase adsorption, and glucan synthesis of Streptococcus mutans AHT mutants
Monsan et al. On the production of dextran by free and immobilized dextransucrase
US4250262A (en) Method of preparing a purified glucosyltransferase
Carey et al. Soluble group-and type-specific antigens from type III group B Streptococcus
Bammann et al. Immunoglobulin A antibodies reactive with Streptococcus mutans in saliva of adults, children, and predentate infants
US5824521A (en) Saccharide composition containing trehalulose, its preparation and uses
EP0096547B1 (en) Process for preparing uridine diphosphate-n-acetylgalactosamine
Yamashita et al. Purification of a fourth glucosyltransferase from Streptococcus sobrinus
Renosto et al. Comparative stability and catalytic and chemical properties of the sulfate-activating enzymes from Penicillium chrysogenum (mesophile) and Penicillium duponti (thermophile)
US5843748A (en) Trehalose phosphorylase its preparation and uses
Taniguchi et al. Purification and characterization of UDP-N-acetylgalactosamine: globotriaosylceramide beta-3-N-acetylgalactosaminyltransferase, a synthase of human blood group P antigen, from canine spleen.
US5281524A (en) Cell-associated glucosyltransferase from Streptococcus mutans serotype, c, e or f
Henry et al. The distribution of fructan metabolising enzymes in the onion plant
Okahashi et al. Purification and immunochemical characterization of Streptococcus sanguis serotype I carbohydrate antigen
Yamashita et al. Evidence for the presence of two distinct sites of sucrose hydrolysis and glucosyl transfer activities on 1, 3‐α‐D‐glucan synthase of Streptococcus mutans
Choi et al. Isolation and characterization of a novel intracellular glucosyltransferase from the acarbose producer Actinoplanes sp. CKD485-16
Ritz et al. Effects of trypsin treatment on the structure and function of solubilized coupling factor-latent ATPase from Mycobacterium phlei
Shizukuishi et al. α-l-fucosidase activity of some oral streptococci
Kessler et al. Interaction of anti-kojibiose antibody with the lipoteichoic acids from Streptococcus faecalis and Streptococcus faecium
Wickner et al. [149d] N-formimino-l-glutamate iminohydrolase (Pseudomonas sp.)

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
G160 Decision to publish patent application
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20081001

Year of fee payment: 15

EXPY Expiration of term