KR890011996A - 세포연관 글루코실트랜스퍼라제, 그의 항체 및 유효성분으로서 상기 항체를 함유하는 이의 카리에스 예방조성물 - Google Patents
세포연관 글루코실트랜스퍼라제, 그의 항체 및 유효성분으로서 상기 항체를 함유하는 이의 카리에스 예방조성물 Download PDFInfo
- Publication number
- KR890011996A KR890011996A KR1019890000697A KR890000697A KR890011996A KR 890011996 A KR890011996 A KR 890011996A KR 1019890000697 A KR1019890000697 A KR 1019890000697A KR 890000697 A KR890000697 A KR 890000697A KR 890011996 A KR890011996 A KR 890011996A
- Authority
- KR
- South Korea
- Prior art keywords
- glucosyltransferase
- antibody
- cell
- linked
- caries
- Prior art date
Links
- 102000000340 Glucosyltransferases Human genes 0.000 title claims 11
- 108010055629 Glucosyltransferases Proteins 0.000 title claims 11
- 208000002925 dental caries Diseases 0.000 title claims 5
- 239000004480 active ingredient Substances 0.000 title claims 2
- 230000003449 preventive effect Effects 0.000 title claims 2
- 230000000694 effects Effects 0.000 claims description 6
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 2
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 claims 7
- 229920001503 Glucan Polymers 0.000 claims 4
- 238000000034 method Methods 0.000 claims 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 4
- 230000001900 immune effect Effects 0.000 claims 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 2
- 229930006000 Sucrose Natural products 0.000 claims 2
- 230000015572 biosynthetic process Effects 0.000 claims 2
- 210000004748 cultured cell Anatomy 0.000 claims 2
- 235000013601 eggs Nutrition 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 239000005720 sucrose Substances 0.000 claims 2
- 238000003786 synthesis reaction Methods 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 241001465754 Metazoa Species 0.000 claims 1
- 241000194019 Streptococcus mutans Species 0.000 claims 1
- 150000001450 anions Chemical class 0.000 claims 1
- 230000000890 antigenic effect Effects 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 230000001747 exhibiting effect Effects 0.000 claims 1
- 238000010438 heat treatment Methods 0.000 claims 1
- 230000003053 immunization Effects 0.000 claims 1
- 230000002265 prevention Effects 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- 210000002966 serum Anatomy 0.000 claims 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims 1
- 238000001179 sorption measurement Methods 0.000 claims 1
- 230000002194 synthesizing effect Effects 0.000 claims 1
- 101710205482 Nuclear factor 1 A-type Proteins 0.000 description 2
- 101710170464 Nuclear factor 1 B-type Proteins 0.000 description 2
- 102100022162 Nuclear factor 1 C-type Human genes 0.000 description 2
- 101710113455 Nuclear factor 1 C-type Proteins 0.000 description 2
- 101710140810 Nuclear factor 1 X-type Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
- C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
- C07K16/1275—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Streptococcus (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/46—Streptococcus ; Enterococcus; Lactococcus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/885—Streptococcus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/827—Proteins from mammals or birds
- Y10S530/85—Reproductive organs or embryos
- Y10S530/852—Sperm
- Y10S530/853—Ovary; eggs; embryos
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Virology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Tropical Medicine & Parasitology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Cosmetics (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Dental Preparations (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 실시예3 에서의 DEAE-세파셀로부터 용출된 부분에서의 단백질 농도(A280), CTF 활성 및 FTase(D-프록토실트랜스퍼라제)활성을 나타낸다.
제2도는 실시예3에서의 히드록실아파타이트로부터 용출된 부분에서의 단백질 농도(A280), CTF 활성 및 FTase활성을 나타낸다.
제3도는 실시예3 에서의 면역된 암탉의 항체 역가의 변화를 나타낸다.
Claims (7)
- a) 수크로스와 반응하고 물에 불용성인 글루칸을 합성하며; b) 6.7 과 7.0사이에서 최적 pH를 가지며; c) 15와 50℃사이에서 최적 온도를 가지며;d) 80℃에서 5분간 열처리로 그의 활성을 상실하며; e) SDS-폴리아크릴아미드겔 전기영동으로 측정하여 150 내지 165 킬로달톤의 분자량을 가지며; f) 효소 그 자체에 특이적인 항체를 생성시키는 동물에 항원성을 가지는 생리화학적 특성을 갖는 세포 연관 글루코실트랜 스퍼라제.
- 수크로스로부터 물에 불용성인 글루칸을 합성하는 반응을 촉매하는 세포연관 글루코실트랜스퍼라제를 생성하는 능력을 가지는 혈청 c,e 또는 f중의 하나의 에스 무탄스의 배양세포로부터 글루코실트랜스퍼라제 활성을 추출하고, 두 흡착과정, 즉 음이온 교환기를 사용하는 하나와 히드록실아파타이트를 사용하는 다른 하나로 구성된 방법에 의해 글루코실트랜스퍼라제를 정제하는 단계를 특징으로하는 제1항에 따른 세포 연관 글루코실트랜스퍼라제의 분리 및 정제방법.
- 스크로스로부터 물에 불용성인 글루칸을 합성하는 반응을 촉매하는 세포연관 글루코실트랜스퍼라제를 생성하는 능력을 갖는 혈청형 c,e 또는 f중의 하나의 에스 무탄스를 배양하고, 수득한 배양 세포로 부터 제1항에 따른 분리한 세포연관 글루코실트랜스퍼라제를 제조하는 단계를 특징으로하는 물에 불용성인 글루칸을 합성하는 효소 활성을 갖는 세포연관 글루코실트랜스퍼라제의 생성방법.
- 제1항에 따른 분리한 세포 연관 글루코실트랜스퍼라제로써 면역화한 암탉에 의해 생성된 계란으로부터 제조하며 이의 카리에스를 생성하는 혈청형 c,e 또는 f의 에스 무탄스에 대하여 면역학적 활성을 나타내는 항체.
- 제1항에 따른 분리한 세포 연관 글루코실트랜스퍼라제로써 암탉을 면역화하고, 면역화된 암탉의 계란으로부터 상기 글루코실트랜스퍼라제에 대하여 면역학적 활성을 갖는 임무노글로볼린을 제조하는 단계를 특징으로하는, 이의 카리에스를 생성하는 혈청형 c,e 또는 f의 이의 에스 무탄스에 대하여 면역학적 활성을 나타내는 항체의 제조방법.
- 유효성분으로서 제4항에 따른 항체를 함유하는 이의 카리에스 예방조성물.
- 제4항에 따른 항체와 적어도 담체를 혼합하는 단계를 포함하는 이의 카리에스 예방조성물의 제조방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP63010853A JP2641228B2 (ja) | 1988-01-22 | 1988-01-22 | 抗体およびそれを有効成分とするう蝕予防剤、並びにこれらの製造方法 |
JP63-10853 | 1988-01-22 | ||
JP10853/88 | 1988-01-22 |
Publications (2)
Publication Number | Publication Date |
---|---|
KR890011996A true KR890011996A (ko) | 1989-08-23 |
KR940010861B1 KR940010861B1 (ko) | 1994-11-18 |
Family
ID=11761913
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019890000697A KR940010861B1 (ko) | 1988-01-22 | 1989-01-23 | 세포연관 글루코실트랜스퍼라제, 그의 항체 및 유효성분으로서 상기 항체를 함유하는 이의 카리에스 예방 조성물 |
Country Status (7)
Country | Link |
---|---|
US (1) | US5439680A (ko) |
EP (1) | EP0334467B1 (ko) |
JP (1) | JP2641228B2 (ko) |
KR (1) | KR940010861B1 (ko) |
CN (1) | CN1033865C (ko) |
CA (1) | CA1325192C (ko) |
DE (1) | DE68921908T2 (ko) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2689511B2 (ja) * | 1988-08-18 | 1997-12-10 | ライオン株式会社 | 食 品 |
GB9409387D0 (en) * | 1994-05-11 | 1994-06-29 | Unilever Plc | Glucan-binding domains (gbp's) and hybird proteins containing gbd's as novel active systems targeted to dental plaque |
RU2122520C1 (ru) * | 1996-10-31 | 1998-11-27 | Акционерное общество закрытого типа "ОСТИМ" | Способ получения суспензии гидроксиапатита |
US5952205A (en) | 1998-02-06 | 1999-09-14 | Neose Technologies, Inc. | Process for processing sucrose into glucose and fructose |
US6231857B1 (en) | 1998-09-28 | 2001-05-15 | The Regents Of The University Of California | Antibodies to S. mutans and uses thereof |
EP1313506A1 (en) * | 2000-08-24 | 2003-05-28 | Washington Dental Service | Immunologic method for the prevention of dental caries |
US7875598B2 (en) | 2004-03-04 | 2011-01-25 | The Regents Of The University Of California | Compositions useful for the treatment of microbial infections |
US7517541B2 (en) | 2005-01-18 | 2009-04-14 | A.M. Todd Company | Oral care compositions derived from the Labiatae family |
WO2008030988A2 (en) | 2006-09-06 | 2008-03-13 | The Regents Of The University Of California | Selectively targeted antimicrobial peptides and the use thereof |
US20080311129A1 (en) * | 2006-11-02 | 2008-12-18 | Camas Incorporated | Adherence inhibitor directed to and method of making and using |
JP4982636B2 (ja) * | 2007-03-29 | 2012-07-25 | 株式会社ファーマフーズ | 虫歯予防用組成物 |
JP5868103B2 (ja) | 2011-09-30 | 2016-02-24 | 日本ゼトック株式会社 | 口腔用組成物 |
CN109593129B (zh) * | 2018-12-19 | 2022-09-06 | 广东工业大学 | 一种用于预防龋齿的卵黄抗体及其制备方法和卵黄抗体制剂 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1505513A (en) * | 1975-05-23 | 1978-03-30 | Stolle Res & Dev | Dental caries inhibiting product |
US4150116A (en) * | 1978-02-21 | 1979-04-17 | Forsyth Dental Infirmary For Children | Immunization against dental caries with glucosyltransferase antigens |
JPS56145222A (en) * | 1980-04-28 | 1981-11-11 | Toshiyuki Hamaoka | Improved antibody and its preparation |
US4748018A (en) * | 1984-02-07 | 1988-05-31 | Stolle Research & Development Corp. | Method of passive immunization of mammals using avian antibody |
US4693888A (en) * | 1983-08-11 | 1987-09-15 | Lion Corporation | Caries-preventive composition |
JPS6038327A (ja) * | 1983-08-11 | 1985-02-27 | Lion Corp | う蝕予防剤 |
JPH0641424B2 (ja) * | 1984-11-06 | 1994-06-01 | ライオン株式会社 | う蝕予防剤 |
JPS62223112A (ja) * | 1986-03-25 | 1987-10-01 | Rooto Seiyaku Kk | 歯周病治療剤 |
JPH0728339B2 (ja) * | 1986-07-01 | 1995-03-29 | 富士通株式会社 | 代表選択方式 |
US5281524A (en) * | 1988-01-22 | 1994-01-25 | Kanebo, Ltd. | Cell-associated glucosyltransferase from Streptococcus mutans serotype, c, e or f |
US5281746A (en) * | 1993-02-01 | 1994-01-25 | Monsanto Company | Process for preparing peroxyacid precursors having amide moieties in the fatty chain |
-
1988
- 1988-01-22 JP JP63010853A patent/JP2641228B2/ja not_active Expired - Lifetime
-
1989
- 1989-01-18 CA CA000588547A patent/CA1325192C/en not_active Expired - Lifetime
- 1989-01-19 EP EP89300488A patent/EP0334467B1/en not_active Expired - Lifetime
- 1989-01-19 DE DE68921908T patent/DE68921908T2/de not_active Expired - Lifetime
- 1989-01-21 CN CN89101420A patent/CN1033865C/zh not_active Expired - Lifetime
- 1989-01-23 KR KR1019890000697A patent/KR940010861B1/ko not_active IP Right Cessation
-
1994
- 1994-12-02 US US08/353,257 patent/US5439680A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
KR940010861B1 (ko) | 1994-11-18 |
DE68921908D1 (de) | 1995-05-04 |
EP0334467A2 (en) | 1989-09-27 |
CA1325192C (en) | 1993-12-14 |
JP2641228B2 (ja) | 1997-08-13 |
CN1033865C (zh) | 1997-01-22 |
DE68921908T2 (de) | 1995-07-27 |
JPH01190635A (ja) | 1989-07-31 |
EP0334467B1 (en) | 1995-03-29 |
US5439680A (en) | 1995-08-08 |
EP0334467A3 (en) | 1990-06-06 |
CN1036036A (zh) | 1989-10-04 |
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