KR890011996A - 세포연관 글루코실트랜스퍼라제, 그의 항체 및 유효성분으로서 상기 항체를 함유하는 이의 카리에스 예방조성물 - Google Patents

세포연관 글루코실트랜스퍼라제, 그의 항체 및 유효성분으로서 상기 항체를 함유하는 이의 카리에스 예방조성물 Download PDF

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KR890011996A
KR890011996A KR1019890000697A KR890000697A KR890011996A KR 890011996 A KR890011996 A KR 890011996A KR 1019890000697 A KR1019890000697 A KR 1019890000697A KR 890000697 A KR890000697 A KR 890000697A KR 890011996 A KR890011996 A KR 890011996A
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glucosyltransferase
antibody
cell
linked
caries
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KR1019890000697A
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KR940010861B1 (ko
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도시오 호리꼬시
주니찌로 히라오까
이사무 후지따
도루 도꼬로
요시까쯔 고다마
히데아끼 요꼬야마
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가네보가부시끼가이샤
겐 코포레이션
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    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
    • C07K16/1275—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Streptococcus (G)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
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    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/46—Streptococcus ; Enterococcus; Lactococcus
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Abstract

내용 없음

Description

세포연관 글루코실트랜스퍼라제, 그의 항체 및 유효성분으로서 상기 항체를 함유하는 이의 카리에스 예방조성물
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 실시예3 에서의 DEAE-세파셀로부터 용출된 부분에서의 단백질 농도(A280), CTF 활성 및 FTase(D-프록토실트랜스퍼라제)활성을 나타낸다.
제2도는 실시예3에서의 히드록실아파타이트로부터 용출된 부분에서의 단백질 농도(A280), CTF 활성 및 FTase활성을 나타낸다.
제3도는 실시예3 에서의 면역된 암탉의 항체 역가의 변화를 나타낸다.

Claims (7)

  1. a) 수크로스와 반응하고 물에 불용성인 글루칸을 합성하며; b) 6.7 과 7.0사이에서 최적 pH를 가지며; c) 15와 50℃사이에서 최적 온도를 가지며;d) 80℃에서 5분간 열처리로 그의 활성을 상실하며; e) SDS-폴리아크릴아미드겔 전기영동으로 측정하여 150 내지 165 킬로달톤의 분자량을 가지며; f) 효소 그 자체에 특이적인 항체를 생성시키는 동물에 항원성을 가지는 생리화학적 특성을 갖는 세포 연관 글루코실트랜 스퍼라제.
  2. 수크로스로부터 물에 불용성인 글루칸을 합성하는 반응을 촉매하는 세포연관 글루코실트랜스퍼라제를 생성하는 능력을 가지는 혈청 c,e 또는 f중의 하나의 에스 무탄스의 배양세포로부터 글루코실트랜스퍼라제 활성을 추출하고, 두 흡착과정, 즉 음이온 교환기를 사용하는 하나와 히드록실아파타이트를 사용하는 다른 하나로 구성된 방법에 의해 글루코실트랜스퍼라제를 정제하는 단계를 특징으로하는 제1항에 따른 세포 연관 글루코실트랜스퍼라제의 분리 및 정제방법.
  3. 스크로스로부터 물에 불용성인 글루칸을 합성하는 반응을 촉매하는 세포연관 글루코실트랜스퍼라제를 생성하는 능력을 갖는 혈청형 c,e 또는 f중의 하나의 에스 무탄스를 배양하고, 수득한 배양 세포로 부터 제1항에 따른 분리한 세포연관 글루코실트랜스퍼라제를 제조하는 단계를 특징으로하는 물에 불용성인 글루칸을 합성하는 효소 활성을 갖는 세포연관 글루코실트랜스퍼라제의 생성방법.
  4. 제1항에 따른 분리한 세포 연관 글루코실트랜스퍼라제로써 면역화한 암탉에 의해 생성된 계란으로부터 제조하며 이의 카리에스를 생성하는 혈청형 c,e 또는 f의 에스 무탄스에 대하여 면역학적 활성을 나타내는 항체.
  5. 제1항에 따른 분리한 세포 연관 글루코실트랜스퍼라제로써 암탉을 면역화하고, 면역화된 암탉의 계란으로부터 상기 글루코실트랜스퍼라제에 대하여 면역학적 활성을 갖는 임무노글로볼린을 제조하는 단계를 특징으로하는, 이의 카리에스를 생성하는 혈청형 c,e 또는 f의 이의 에스 무탄스에 대하여 면역학적 활성을 나타내는 항체의 제조방법.
  6. 유효성분으로서 제4항에 따른 항체를 함유하는 이의 카리에스 예방조성물.
  7. 제4항에 따른 항체와 적어도 담체를 혼합하는 단계를 포함하는 이의 카리에스 예방조성물의 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890000697A 1988-01-22 1989-01-23 세포연관 글루코실트랜스퍼라제, 그의 항체 및 유효성분으로서 상기 항체를 함유하는 이의 카리에스 예방 조성물 KR940010861B1 (ko)

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JP63010853A JP2641228B2 (ja) 1988-01-22 1988-01-22 抗体およびそれを有効成分とするう蝕予防剤、並びにこれらの製造方法
JP63-10853 1988-01-22
JP10853/88 1988-01-22

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KR890011996A true KR890011996A (ko) 1989-08-23
KR940010861B1 KR940010861B1 (ko) 1994-11-18

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US (1) US5439680A (ko)
EP (1) EP0334467B1 (ko)
JP (1) JP2641228B2 (ko)
KR (1) KR940010861B1 (ko)
CN (1) CN1033865C (ko)
CA (1) CA1325192C (ko)
DE (1) DE68921908T2 (ko)

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RU2122520C1 (ru) * 1996-10-31 1998-11-27 Акционерное общество закрытого типа "ОСТИМ" Способ получения суспензии гидроксиапатита
US5952205A (en) 1998-02-06 1999-09-14 Neose Technologies, Inc. Process for processing sucrose into glucose and fructose
US6231857B1 (en) 1998-09-28 2001-05-15 The Regents Of The University Of California Antibodies to S. mutans and uses thereof
EP1313506A1 (en) * 2000-08-24 2003-05-28 Washington Dental Service Immunologic method for the prevention of dental caries
US7875598B2 (en) 2004-03-04 2011-01-25 The Regents Of The University Of California Compositions useful for the treatment of microbial infections
US7517541B2 (en) 2005-01-18 2009-04-14 A.M. Todd Company Oral care compositions derived from the Labiatae family
WO2008030988A2 (en) 2006-09-06 2008-03-13 The Regents Of The University Of California Selectively targeted antimicrobial peptides and the use thereof
US20080311129A1 (en) * 2006-11-02 2008-12-18 Camas Incorporated Adherence inhibitor directed to and method of making and using
JP4982636B2 (ja) * 2007-03-29 2012-07-25 株式会社ファーマフーズ 虫歯予防用組成物
JP5868103B2 (ja) 2011-09-30 2016-02-24 日本ゼトック株式会社 口腔用組成物
CN109593129B (zh) * 2018-12-19 2022-09-06 广东工业大学 一种用于预防龋齿的卵黄抗体及其制备方法和卵黄抗体制剂

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GB1505513A (en) * 1975-05-23 1978-03-30 Stolle Res & Dev Dental caries inhibiting product
US4150116A (en) * 1978-02-21 1979-04-17 Forsyth Dental Infirmary For Children Immunization against dental caries with glucosyltransferase antigens
JPS56145222A (en) * 1980-04-28 1981-11-11 Toshiyuki Hamaoka Improved antibody and its preparation
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JPH0641424B2 (ja) * 1984-11-06 1994-06-01 ライオン株式会社 う蝕予防剤
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US5281746A (en) * 1993-02-01 1994-01-25 Monsanto Company Process for preparing peroxyacid precursors having amide moieties in the fatty chain

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KR940010861B1 (ko) 1994-11-18
DE68921908D1 (de) 1995-05-04
EP0334467A2 (en) 1989-09-27
CA1325192C (en) 1993-12-14
JP2641228B2 (ja) 1997-08-13
CN1033865C (zh) 1997-01-22
DE68921908T2 (de) 1995-07-27
JPH01190635A (ja) 1989-07-31
EP0334467B1 (en) 1995-03-29
US5439680A (en) 1995-08-08
EP0334467A3 (en) 1990-06-06
CN1036036A (zh) 1989-10-04

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