KR890002401A - 재조합 숙주로부터 제조한 아프로티닌 동족체, 그의 표현 벡타 및 재조합 숙주 및 그의 제약상 용도 - Google Patents

재조합 숙주로부터 제조한 아프로티닌 동족체, 그의 표현 벡타 및 재조합 숙주 및 그의 제약상 용도 Download PDF

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KR890002401A
KR890002401A KR1019870007966A KR870007966A KR890002401A KR 890002401 A KR890002401 A KR 890002401A KR 1019870007966 A KR1019870007966 A KR 1019870007966A KR 870007966 A KR870007966 A KR 870007966A KR 890002401 A KR890002401 A KR 890002401A
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aprotinin
microbially
glu
prepared
dna
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KR960007194B1 (ko
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아우엘스발트 에른스트-아우구스트
쉬뢰더 베르너
쉬나벨 에우겐
브룬스 볼프강
라인하르르 겔트
코릭 마이클
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클라우스 데너·귄터 슈마커
바이엘 악티엔게젤샤프트
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Abstract

내용 없음

Description

재조합 숙주로부터 제조한 아프로티닌 동족체, 그의 표현 벡타 및 재조합 숙주 및 그의 제약상 용도
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 항상 아프로티닌 주역 유전자의 디자인을 나타냄.
제2도는 4개의 블톡(알파, 베타, 감마, 델타)으로 되는 합성유전자의 구성에 사용되는 올리고뉴클레오티드의 DNA 서열을 나타냄, 제2b도는 15위치에서 아미노산 변경의 암호가 되는 단편의 DNA 서열을 나타냄.
제3도는 함성 Glu-52-아프로티닌 유전자의 DNA 서열(EcoRI-BamHI, pRK 63.1.1의 부분 서열)을 나타냄.

Claims (23)

  1. 미생물적으로 제조한 아프로티닌 및 아프로티닌 동족체.
  2. 필요에 따라서 메티오닌을 제외한 임의의 자연적으로 발생하는 아미노산에 의해서 15위치에서 치환된, 미생물적으로 제조한 아프로티닌.
  3. 미생물적으로 제조한 아프로티닌 및 Arg-15-, Val-15-, Ile-15-, Leu-15, Phe-15-1, Gly-15-, Ser-15-,Tyr-15- 및 Ala-15-아프로티닌.
  4. 제2항에 있어서, 52위치에서 Glu, Leu, Val, Thr 또는 Ser에 의해 추가로 치환된, 미생물적으로 제조한 아프로티닌.
  5. 미생물적으로 제조한 Glu-52-아프로티닌.
  6. 미생물적으로 제조한 Val-15-Glu-52-아프로티닌.
  7. 미생물적으로 제조한 Ile-15-Glu-52-아프로티닌.
  8. 미생물적으로 제조한 Leu-15-Glu-52-아프로티닌.
  9. 아프로티닌 및(또는) 아프로티닌 동족체를 암호화하는 주역유전자의 구성에 적합한 DNA 단편.
  10. 다음과 같은 군 및 그의 기능상 균등물 중에서 선택되는 DNA 단편.
  11. 다음과 같은 서열 및 그의 기능상 균등물에 의해 특징지워지는 DNA.
  12. 제11항에 있어서, 주로 Lys-15-토든이 Val-15-, Ile-15-, Leu-15-, Phe-15-1, Gly-15-, Ala-15-, Arg-52-, Ser-15-,Trp-15- 또는 Tyr-15-Glu-52-아프로티닌을 암호화하는 코든에 의해 치환된 DNA 서열로 되는 DNA.
  13. 제11항에 및 제12항 중 어느 하나의 항에 있어서, Glu-52-코든이 Leu, Val, Thr 또는 Ser의 암호가 되는 코든에 의해 치환된 DNA.
  14. 플라스미드PRK 63.1.1,PRK 54.1.1,PRK 49.2.1 및PRK 48.1.1.
  15. 제1항 내지 제8항의 단백질 제조에 유용한 표현 플라스미드.
  16. PRK 48.1.1 및PRK 49.2.1로 되는 군 중에서 선택되는, 제5항 내지 제8항의 단백질 제조에 유용한 표현 플라스미드.
  17. 아프로티닌 및(또는) 아프로티닌 동족체의 암호가 되는 표현 운반자를 사용하여 형질 전환시킨 숙주세포.
  18. 아프로티닌 및(또는) 아프로티닌 동족체의 암호가 되는 표현 운반자를 사용하여 형질 전환시킨 대장균.
  19. 제16항에 의한 표현 운반자를 사용하여 형질전환시킨 대장균.
  20. 제16항에 의한 표현 운반자를 사용하여 형질전환시킨 대장균 RP 1△M15(ATCC 번호 34102).
  21. 미생물적으로 제조한 아프로티닌 및(또는) 아프로티닌 동족체를 함유하는 제약 조성물.
  22. 미생물적으로 제조한 아프로티닌 및(또는) 아프로티닌 동족체의 약제 제조 용도.
  23. 제9항에 의한 DNA단편의 아프로티닌 및(또는) 아프로티닌 동족체를 암호화하는 유전자 구성 용도.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019870007966A 1986-03-26 1987-07-22 재조합 숙주로부터 제조한 아프로티닌 동족체, 그의 발현 벡타 및 재조합 숙주 및 그의 제약상 용도 KR960007194B1 (ko)

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GB08607523A GB2188322A (en) 1986-03-26 1986-03-26 Aprotinin and analogues thereof produced by a recombinant host
GB8607523 1986-03-26

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KR1019870002715A KR950000302B1 (ko) 1986-03-26 1987-03-25 아프로티닌 및 아프로티닌 동족체의 제조 방법
KR1019870007966A KR960007194B1 (ko) 1986-03-26 1987-07-22 재조합 숙주로부터 제조한 아프로티닌 동족체, 그의 발현 벡타 및 재조합 숙주 및 그의 제약상 용도

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US (1) US4894436A (ko)
EP (1) EP0238993B1 (ko)
JP (1) JP2537507B2 (ko)
KR (2) KR950000302B1 (ko)
CN (1) CN1018793B (ko)
AT (1) ATE123301T1 (ko)
AU (1) AU600475B2 (ko)
CA (1) CA1338309C (ko)
DE (1) DE3751324D1 (ko)
DK (1) DK151687A (ko)
ES (1) ES2073390T3 (ko)
FI (1) FI94876C (ko)
GB (1) GB2188322A (ko)
IL (1) IL81964A (ko)
ZA (1) ZA872181B (ko)

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GB2188322A (en) 1987-09-30
EP0238993A2 (de) 1987-09-30
KR960007194B1 (ko) 1996-05-29
ES2073390T3 (es) 1995-08-16
ATE123301T1 (de) 1995-06-15
KR870009025A (ko) 1987-10-22
EP0238993A3 (en) 1989-03-22
CN1018793B (zh) 1992-10-28
JP2537507B2 (ja) 1996-09-25
AU6990187A (en) 1987-10-01
IL81964A (en) 1993-07-08
CA1338309C (en) 1996-05-07
ZA872181B (en) 1987-12-30
DK151687D0 (da) 1987-03-25
FI871288A0 (fi) 1987-03-24
FI871288A (fi) 1987-09-27
FI94876B (fi) 1995-07-31
GB8607523D0 (en) 1986-04-30
DK151687A (da) 1987-09-27
US4894436A (en) 1990-01-16
IL81964A0 (en) 1987-10-20
JPS637794A (ja) 1988-01-13
KR950000302B1 (ko) 1995-01-13
FI94876C (fi) 1995-11-10
DE3751324D1 (de) 1995-07-06
CN87102412A (zh) 1987-10-07
AU600475B2 (en) 1990-08-16
EP0238993B1 (de) 1995-05-31

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