KR880007723A - 합성유전자에 의한 효모세포로부터 돼지 성장호르몬의 제조방법 - Google Patents

합성유전자에 의한 효모세포로부터 돼지 성장호르몬의 제조방법 Download PDF

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KR880007723A
KR880007723A KR860011710A KR860011710A KR880007723A KR 880007723 A KR880007723 A KR 880007723A KR 860011710 A KR860011710 A KR 860011710A KR 860011710 A KR860011710 A KR 860011710A KR 880007723 A KR880007723 A KR 880007723A
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Prior art keywords
growth hormone
pig growth
gene
xba
sal
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KR860011710A
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KR920003663B1 (ko
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조중명
이태호
정현호
이용범
이태규
박영우
한규범
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허신구
주식회사 럭키
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Priority to KR1019860011710A priority Critical patent/KR920003663B1/ko
Priority to PCT/KR1987/000015 priority patent/WO1988005080A1/en
Priority to EP88900598A priority patent/EP0295287B1/en
Priority to AT88900598T priority patent/ATE92958T1/de
Priority to JP63500757A priority patent/JPH0712317B2/ja
Priority to DE88900598T priority patent/DE3787016T2/de
Publication of KR880007723A publication Critical patent/KR880007723A/ko
Priority to US07/613,938 priority patent/US5541086A/en
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Publication of KR920003663B1 publication Critical patent/KR920003663B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin

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  • Health & Medical Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
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Abstract

내용 없음.

Description

합성유전자에 의한 효모세포로부터 돼지 성장호르몬의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 전체 돼지 성장호르몬 유전자에 해당하는 28종류의 합성 올리고 뉴클리오티드의 염기순서를 5′ 말단에서 3′말단순서로 나타낸 것이며,
제2도는 제1도의 올리고 뉴클리오티드의 접합전략을 도시한 것이며,
제3도는 합성 올리고 뉴클리오티드를 대장균 운반 pUC18에로의 클로닝 과정을 도시한 것이며,
제4도는 확인한 돼지 성장호르몬의 염기 서열 및 아미노산 서열을 나타낸 것이며,
제5도는 효모내에서 돼지 성장호르몬의 대량 생산을 위하여 대장균 운반체 pPGAP 및 효모발현 운반체 pCl/l에의 클로닝 과정을 도시한 것이다.

Claims (2)

  1. 돼지 성장호르몬의 아미노산 배열순서에 따라 효모에서 선택적으로 사용되는 코돈 및 Sac I, Xba I 및 Sal I의 인지부위가 존재하는 올리고 뉴클리오티드를 합성한 후 이를 접합전략에 따라 접합시켜 합성올리고 뉴클리오티드의 카복시쪽 Xba I/Sal I 절편을 Xba I/Sal I으로 처리한 대장균 운반체(pUC18)에 클로닝한 다음 이를 Sac I/Xba I으로 처리하여 합성 올리고 뉴클리오티디의 아미노쪽 Sac I/Xba I 절편을 클로닝시켜 아미노 말단의 일부가 없는 돼지 성장호르몬 유전자를 제조한 다음 이 유전자를 아미노말단 합성유전자와 함께 프로모터와 터니미네이터가 있는 대장균 운반체에 클로닝하여 포로모터-전체 돼지 성장 호르몬 유전자-터미네이터의 카세트를 제조한 다음 이를 효모발현 운반체에 재클로닝시켜 최종 효모발현 운반체를 만들어 이를 효모내에서 발현시킴을 특징으로 하는 돼지 성장호르몬의 제조방법.
  2. 제1항에 있어서, 아미노 말단 합성 유전자는 Nco I 인지부위 및 Sal I 인지부위가 존재하는
    임을 특징으로 하는 돼지 성장호르몬의 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019860011710A 1986-12-31 1986-12-31 합성유전자에 의한 효모세포로부터 돼지 성장 호르몬의 제조방법 KR920003663B1 (ko)

Priority Applications (7)

Application Number Priority Date Filing Date Title
KR1019860011710A KR920003663B1 (ko) 1986-12-31 1986-12-31 합성유전자에 의한 효모세포로부터 돼지 성장 호르몬의 제조방법
PCT/KR1987/000015 WO1988005080A1 (en) 1986-12-31 1987-12-28 Method for the production of porcine growth hormone using a synthetic gene in yeast cells
EP88900598A EP0295287B1 (en) 1986-12-31 1987-12-28 Method for the production of porcine growth hormone using a synthetic gene in yeast cells
AT88900598T ATE92958T1 (de) 1986-12-31 1987-12-28 Verfahren zur herstellung des schweinewachstumshormons mittels eines kunstgens in hefezellen.
JP63500757A JPH0712317B2 (ja) 1986-12-31 1987-12-28 合成遺伝子を使用した酵母細胞でのブタ成長ホルモンの生産方法
DE88900598T DE3787016T2 (de) 1986-12-31 1987-12-28 Verfahren zur herstellung des schweinewachstumshormons mittels eines kunstgens in hefezellen.
US07/613,938 US5541086A (en) 1986-12-31 1990-11-13 Method for the production of porcine growth hormone using a synthetic gene in yeast cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019860011710A KR920003663B1 (ko) 1986-12-31 1986-12-31 합성유전자에 의한 효모세포로부터 돼지 성장 호르몬의 제조방법

Publications (2)

Publication Number Publication Date
KR880007723A true KR880007723A (ko) 1988-08-29
KR920003663B1 KR920003663B1 (ko) 1992-05-06

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Application Number Title Priority Date Filing Date
KR1019860011710A KR920003663B1 (ko) 1986-12-31 1986-12-31 합성유전자에 의한 효모세포로부터 돼지 성장 호르몬의 제조방법

Country Status (6)

Country Link
EP (1) EP0295287B1 (ko)
JP (1) JPH0712317B2 (ko)
KR (1) KR920003663B1 (ko)
AT (1) ATE92958T1 (ko)
DE (1) DE3787016T2 (ko)
WO (1) WO1988005080A1 (ko)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI94278C (fi) * 1992-04-22 1995-08-10 Risto Wisakanto Paineväliaineen käyttö porauksessa
CN109929849B (zh) * 2019-03-18 2021-05-04 华南农业大学 一种优化的pGH基因、蛋白及在提高毕赤酵母分泌表达pGH产量和活性中的应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1341012C (en) * 1982-09-27 2000-06-06 Biogen, Inc. Dna sequences, recombinant dna molecules and processes for producing swine growth hormone-like polypeptides
AU2092983A (en) * 1982-11-08 1984-05-17 Genentech Inc. Porcine growth hormone produced by recombinant dna technology
US4788144A (en) * 1985-06-28 1988-11-29 International Minerals & Chemical Corp. Fermentation process for the high level production of swine growth

Also Published As

Publication number Publication date
EP0295287A1 (en) 1988-12-21
JPH0712317B2 (ja) 1995-02-15
KR920003663B1 (ko) 1992-05-06
ATE92958T1 (de) 1993-08-15
EP0295287B1 (en) 1993-08-11
JPH02501260A (ja) 1990-05-10
DE3787016T2 (de) 1993-11-25
WO1988005080A1 (en) 1988-07-14
DE3787016D1 (ko) 1993-09-16

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