KR860009124A - 원형질체의 배양 방법 - Google Patents

원형질체의 배양 방법 Download PDF

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Publication number
KR860009124A
KR860009124A KR1019860003910A KR860003910A KR860009124A KR 860009124 A KR860009124 A KR 860009124A KR 1019860003910 A KR1019860003910 A KR 1019860003910A KR 860003910 A KR860003910 A KR 860003910A KR 860009124 A KR860009124 A KR 860009124A
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KR
South Korea
Prior art keywords
medium
protoplasts
rice
cultured
control
Prior art date
Application number
KR1019860003910A
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English (en)
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KR890004026B1 (ko
Inventor
다쓰히또 후지무라
모또이 사꾸라이
Original Assignee
미쓰이도오아쓰 가가꾸 가부시끼가이샤
도즈까 야스아끼
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by 미쓰이도오아쓰 가가꾸 가부시끼가이샤, 도즈까 야스아끼 filed Critical 미쓰이도오아쓰 가가꾸 가부시끼가이샤
Publication of KR860009124A publication Critical patent/KR860009124A/ko
Application granted granted Critical
Publication of KR890004026B1 publication Critical patent/KR890004026B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/14—Plant cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

내용 없음

Description

원형질체의 배양 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (12)

  1. 원형질체를 pH가 5.2 이하인 액체 배지에서 배양함을 특징으로 하는 원형질체의 배양 방법.
  2. 제1항에 있어서, 배지의 pH가 3.5∼5.2인 방법.
  3. 제2항에 있어서, 배지의 pH가 4.0∼4.7인 방법.
  4. 제1항에 있어서, 배지가 세포를 배양 하는데 사용하는 배지 25중량％ 이상을 함유하는 조절 배지인 방법.
  5. 제1항에 있어서, 조절 배지가 80중량％ 이상의 사용된 배지를 함유하는 방법.
  6. 제4항에 있어서, 조절배지에 함유된 사용된 배지에서 배양된 세포가 조절 배지에서 배양될 원형질체와 같은 종류인 방법.
  7. 제1항에 있어서, 원형질체가 벼의 원형질체인 방법.
  8. 제4항에 있어서, 원형질체가 벼의 원형질체인 방법.
  9. 제7항에 있어서, 벼가 오리자 사티바인 방법.
  10. 제4항에 있어서, 벼가 오리자 사티바인 방법.
  11. 제7항에 있어서, 배지가 그의 기본 배지로 R2, N6, B5 또는 MS 배지를 함유하는 방법.
  12. 제9항에 있어서, 배지가 0.1∼10mg/ℓ의 2,4-D를 더 함유하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019860003910A 1985-05-21 1986-05-20 원형질체의 배양방법 KR890004026B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP106886 1985-05-21
JP60106886A JPS61265087A (ja) 1985-05-21 1985-05-21 プロトプラストの培養方法

Publications (2)

Publication Number Publication Date
KR860009124A true KR860009124A (ko) 1986-12-20
KR890004026B1 KR890004026B1 (ko) 1989-10-16

Family

ID=14444978

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019860003910A KR890004026B1 (ko) 1985-05-21 1986-05-20 원형질체의 배양방법

Country Status (6)

Country Link
US (1) US4880744A (ko)
EP (1) EP0202669A3 (ko)
JP (1) JPS61265087A (ko)
KR (1) KR890004026B1 (ko)
CN (1) CN86103461B (ko)
CA (1) CA1275957C (ko)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61265086A (ja) * 1985-05-21 1986-11-22 Mitsui Toatsu Chem Inc プロトプラストの培養法
WO2020203947A1 (ja) * 2019-03-29 2020-10-08 キリンホールディングス株式会社 有用物質を蓄積する植物体

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU529693B2 (en) * 1978-08-16 1983-06-16 British Petroleum Company Limited, The Undifferentiated plant cell cultivation
FR2544167B1 (fr) * 1983-04-13 1985-08-09 Paris Xi Labo Amelior Plantes Procede d'obtention d'hybrides somatiques de luzernes par fusion de protoplastes
JPS61265022A (ja) * 1985-05-21 1986-11-22 三井東圧化学株式会社 イネプロトプラストからの植物体の再生方法
JPS61265086A (ja) * 1985-05-21 1986-11-22 Mitsui Toatsu Chem Inc プロトプラストの培養法

Also Published As

Publication number Publication date
US4880744A (en) 1989-11-14
CA1275957C (en) 1990-11-06
JPS61265087A (ja) 1986-11-22
KR890004026B1 (ko) 1989-10-16
CN86103461B (zh) 1988-04-06
EP0202669A2 (en) 1986-11-26
EP0202669A3 (en) 1988-07-27
CN86103461A (zh) 1986-11-26
JPH0533979B2 (ko) 1993-05-20

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