KR860009123A - 원형질체의 배양 방법 - Google Patents

원형질체의 배양 방법 Download PDF

Info

Publication number
KR860009123A
KR860009123A KR1019860003909A KR860003909A KR860009123A KR 860009123 A KR860009123 A KR 860009123A KR 1019860003909 A KR1019860003909 A KR 1019860003909A KR 860003909 A KR860003909 A KR 860003909A KR 860009123 A KR860009123 A KR 860009123A
Authority
KR
South Korea
Prior art keywords
protoplasts
thickness
hydrophilic support
medium
rice
Prior art date
Application number
KR1019860003909A
Other languages
English (en)
Other versions
KR890004025B1 (ko
Inventor
다쓰히또 후지무라
모또이 사꾸라이
Original Assignee
미쓰이도오 아쓰가가꾸 가부시끼가이샤
도즈까 야스아끼
미쓰이도오아쓰 가가꾸 가부시끼가이샤
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 미쓰이도오 아쓰가가꾸 가부시끼가이샤, 도즈까 야스아끼, 미쓰이도오아쓰 가가꾸 가부시끼가이샤 filed Critical 미쓰이도오 아쓰가가꾸 가부시끼가이샤
Publication of KR860009123A publication Critical patent/KR860009123A/ko
Application granted granted Critical
Publication of KR890004025B1 publication Critical patent/KR890004025B1/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/14—Plant cells

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

내용 없음

Description

원형질체의 배양 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (11)

  1. 원형질체를 양 100∼400㎛ 두께의 액체배치층에서 배양함을 특징으로 하는 원형질체의 배양방법.
  2. 제1항에 있어서, 액체 배지층의 두께가 약 200∼300㎛인 방법.
  3. 제1항에 있어서, 액지배지층을 친수성지지체 위에 놓는 방법.
  4. 제3항에 있어서, 친수성 지지체가 한천, 알긴산 및 그의 염, 및 젤라틴의 군에서 선택된 것인 방법.
  5. 제4항에 있어서, 친수성 지지체의 두께가 1mm 이하인 방법.
  6. 제5항에 있어서, 친수성 지지체의 두께가 200㎛ 이하인 방법.
  7. 제1항에 있어서, 원형질체가 벼, 당근 또는 피튜우니어의 원형질체인 방법.
  8. 제7항에 있어서, 원형질체가 벼의 원형질체인 방법.
  9. 제8항에 있어서, 벼가 오리자 사티바인 방법.
  10. 제8항에 있어서, 배지가 그의 기본 배지로 R2, N6, MS 또는 B5배지를 함유하는 방법.
  11. 제9항에 있어서, 배지가 0.1∼10mg/ℓ의 2,4-D를 함유하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019860003909A 1985-05-21 1986-05-20 원형질체의 배양방법 KR890004025B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP60106885A JPS61265086A (ja) 1985-05-21 1985-05-21 プロトプラストの培養法
JP106885 1985-05-21

Publications (2)

Publication Number Publication Date
KR860009123A true KR860009123A (ko) 1986-12-20
KR890004025B1 KR890004025B1 (ko) 1989-10-16

Family

ID=14444950

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019860003909A KR890004025B1 (ko) 1985-05-21 1986-05-20 원형질체의 배양방법

Country Status (6)

Country Link
US (1) US5166068A (ko)
EP (1) EP0202668A3 (ko)
JP (1) JPS61265086A (ko)
KR (1) KR890004025B1 (ko)
CN (1) CN86103374B (ko)
CA (1) CA1274201A (ko)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61265087A (ja) * 1985-05-21 1986-11-22 Mitsui Toatsu Chem Inc プロトプラストの培養方法
JPH05501352A (ja) * 1989-08-09 1993-03-18 ディカルブ　ジェネティクス　コーポレイション 安定な形質転換稔性単子葉植物およびそれらの細胞を製造するための方法および組成物
US6803499B1 (en) 1989-08-09 2004-10-12 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5550318A (en) * 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5484956A (en) * 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US6946587B1 (en) * 1990-01-22 2005-09-20 Dekalb Genetics Corporation Method for preparing fertile transgenic corn plants
CA2074355C (en) * 1990-01-22 2008-10-28 Ronald C. Lundquist Method of producing fertile transgenic corn plants
US6777589B1 (en) 1990-01-22 2004-08-17 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6329574B1 (en) 1990-01-22 2001-12-11 Dekalb Genetics Corporation High lysine fertile transgenic corn plants
US6395966B1 (en) 1990-08-09 2002-05-28 Dekalb Genetics Corp. Fertile transgenic maize plants containing a gene encoding the pat protein
US5780709A (en) * 1993-08-25 1998-07-14 Dekalb Genetics Corporation Transgenic maize with increased mannitol content
US6118047A (en) * 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
AU2009202819B2 (en) 2007-07-13 2014-08-28 Aramis Biotechnologies Inc. Recombinant influenza virus-like particles (VLPs) produced in transgenic plants expressing hemagglutinin
DK2480560T3 (en) * 2009-09-22 2018-04-16 Medicago Inc PROCEDURE FOR THE PREPARATION OF PLANT DERIVATIVE PROTEINS
TWI461527B (zh) * 2012-12-26 2014-11-21 Medium device

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3485610D1 (de) * 1983-08-04 1992-04-30 Ciba Geigy Ag Verfahren zur erhaltung und vermehrung von defekten, nicht-infektioesen virusgenomen.
JPS61265022A (ja) * 1985-05-21 1986-11-22 三井東圧化学株式会社 イネプロトプラストからの植物体の再生方法
JPS61265087A (ja) * 1985-05-21 1986-11-22 Mitsui Toatsu Chem Inc プロトプラストの培養方法

Also Published As

Publication number Publication date
JPH0567277B2 (ko) 1993-09-24
JPS61265086A (ja) 1986-11-22
CN86103374A (zh) 1986-11-26
CA1274201A (en) 1990-09-18
EP0202668A3 (en) 1988-08-03
CN86103374B (zh) 1988-04-06
US5166068A (en) 1992-11-24
KR890004025B1 (ko) 1989-10-16
EP0202668A2 (en) 1986-11-26

Similar Documents

Publication Publication Date Title
KR860009123A (ko) 원형질체의 배양 방법
Walsh et al. Algal bioassays of industrial and energy process effluents
IT8720433V0 (it) Nastro adesivo fluorescente atto all'impiego com evidenziatore.
DE3780208D1 (de) Textverarbeitungsapparat zur verarbeitung von texten gemaess verschiedenen ausgewaehlten textformaten.
IT8619349A0 (it) Procedimento per l'inibizione di microorganismi in idrocarburi liquidi.!
ES2090026T3 (es) Dispositivo para intensificar la fluorescencia y cineticas y metodos para utilizar el dispositivo.
DE3888689D1 (de) Substrate für enzymtests.
FR2641285B1 (fr) Gene de la glucose-6-phosphate deshydrogenase, plasmide et microorganisme le contenant et procede de preparation de la glucose-6-phosphate deshydrogenase
ATE26087T1 (de) Faelschungssicheres dokument und verfahren zu seiner herstellung.
KR870011506A (ko) 패턴 전사방법 및 이 방법에 사용하는 할로겐화은 사진 건판
BR8404194A (pt) Processo industrial para a producao de poliois,especialmente eritritol e/ou ribitol,pela fermentacao aerobica de um agucar apropriado por moniliella tomentosa var.pollinis,e caldo de cultura contendo poliol
DE3767066D1 (de) Druckerband und film zu seiner herstellung.
KR910013083A (ko) 자기기록매체
Kensinger Panoan linguistic, folkloristic and ethnographic research: retrospect and prospect
JPS6420189A (en) Recording material
KR860009124A (ko) 원형질체의 배양 방법
ES516351A0 (es) "dispositivo para la purificacion biologica aerobia de medios, tales como aguas residuales o lodos de reciente formacion y fecales".
KR927003799A (ko) 자가-접합 활성을 갖는 복유전자 알앤에이(rna)의 발현
BR8404195A (pt) Processo industrial para a producao de poliois,especialmente eritritol e/ou ribitol,pela fermentacao aerobica de um agucar apropriado por moniliella tomentosa var.pollinis e caldo de cultura contendo poliol
ITMI931573A1 (it) Procedimento per l'incorporazione e la protezione delle scritture su documenti cartacei in specie assegni bancari
KR870004404A (ko) 자기 기록 매체
Todd Pidginization and creolization
DE3850276D1 (de) Serotonin-Inhibitoren und sie enthaltende pharmazeutische Zusammensetzungen und mikrobielle Verfahren und Organismen für ihre Herstellung.
Gifford British Drums On The Southern Frontier: The Military Colonization of Georgia, 1733-1749
Wurm Language situation, language contacts and contact languages in the Caucasus area

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
G160 Decision to publish patent application
O035 Opposition [patent]: request for opposition

Free format text: OPPOSITION NUMBER: 001989000410; OPPOSITION DATE: 19891215

E701 Decision to grant or registration of patent right
O073 Decision to grant registration after opposition [patent]: decision to grant registration
GRNT Written decision to grant
LAPS Lapse due to unpaid annual fee