KR860001593A - 모노클로날 항체의 제조방법 - Google Patents
모노클로날 항체의 제조방법 Download PDFInfo
- Publication number
- KR860001593A KR860001593A KR1019850005719A KR850005719A KR860001593A KR 860001593 A KR860001593 A KR 860001593A KR 1019850005719 A KR1019850005719 A KR 1019850005719A KR 850005719 A KR850005719 A KR 850005719A KR 860001593 A KR860001593 A KR 860001593A
- Authority
- KR
- South Korea
- Prior art keywords
- cells
- antibody
- cancer
- clones
- blood cells
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title claims 2
- 238000000034 method Methods 0.000 claims 11
- 239000000427 antigen Substances 0.000 claims 9
- 102000036639 antigens Human genes 0.000 claims 9
- 108091007433 antigens Proteins 0.000 claims 9
- 210000004027 cell Anatomy 0.000 claims 8
- 206010009944 Colon cancer Diseases 0.000 claims 4
- 206010061902 Pancreatic neoplasm Diseases 0.000 claims 4
- 210000000601 blood cell Anatomy 0.000 claims 4
- 208000029742 colonic neoplasm Diseases 0.000 claims 4
- 201000007270 liver cancer Diseases 0.000 claims 4
- 208000014018 liver neoplasm Diseases 0.000 claims 4
- 208000015486 malignant pancreatic neoplasm Diseases 0.000 claims 4
- 201000002528 pancreatic cancer Diseases 0.000 claims 4
- 208000008443 pancreatic carcinoma Diseases 0.000 claims 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims 3
- 210000002966 serum Anatomy 0.000 claims 3
- 241000283073 Equus caballus Species 0.000 claims 2
- 241001465754 Metazoa Species 0.000 claims 2
- 206010028980 Neoplasm Diseases 0.000 claims 2
- 206010035226 Plasma cell myeloma Diseases 0.000 claims 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 claims 2
- 201000011510 cancer Diseases 0.000 claims 2
- 210000003743 erythrocyte Anatomy 0.000 claims 2
- 239000002609 medium Substances 0.000 claims 2
- 201000000050 myeloid neoplasm Diseases 0.000 claims 2
- 239000000243 solution Substances 0.000 claims 2
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 claims 1
- TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 claims 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 claims 1
- 241000283707 Capra Species 0.000 claims 1
- 241000287828 Gallus gallus Species 0.000 claims 1
- 241000699666 Mus <mouse, genus> Species 0.000 claims 1
- 241000283973 Oryctolagus cuniculus Species 0.000 claims 1
- 241001494479 Pecora Species 0.000 claims 1
- 239000002202 Polyethylene glycol Substances 0.000 claims 1
- 239000012979 RPMI medium Substances 0.000 claims 1
- 241000700159 Rattus Species 0.000 claims 1
- 206010070834 Sensitisation Diseases 0.000 claims 1
- 238000002835 absorbance Methods 0.000 claims 1
- 229960003896 aminopterin Drugs 0.000 claims 1
- 210000003719 b-lymphocyte Anatomy 0.000 claims 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 claims 1
- 239000000872 buffer Substances 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- 244000309466 calf Species 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 235000013330 chicken meat Nutrition 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 230000001605 fetal effect Effects 0.000 claims 1
- 210000004754 hybrid cell Anatomy 0.000 claims 1
- 239000007928 intraperitoneal injection Substances 0.000 claims 1
- 239000004816 latex Substances 0.000 claims 1
- 229920000126 latex Polymers 0.000 claims 1
- 210000004698 lymphocyte Anatomy 0.000 claims 1
- HFZNPKDYLSOGBE-SCEMAYBHSA-N nrc-12 Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(C)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)CN)C(C)C)[C@@H](C)O)C(C)C)C(C)C)C1=CC=C(O)C=C1 HFZNPKDYLSOGBE-SCEMAYBHSA-N 0.000 claims 1
- 229920001223 polyethylene glycol Polymers 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 230000008313 sensitization Effects 0.000 claims 1
- 230000001235 sensitizing effect Effects 0.000 claims 1
- 210000004988 splenocyte Anatomy 0.000 claims 1
- 239000000725 suspension Substances 0.000 claims 1
- 229940104230 thymidine Drugs 0.000 claims 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
- C07K16/3046—Stomach, Intestines
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
- C07K16/303—Liver or Pancreas
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (13)
- 항체 형성 세포를 골수종 세포와 융합시켜 잡종 세포를 형성하고, 이 잡종 세포를 클론화하여 췌장암 관련 항원, 대장암 관련 항원 및 간암 관련 항원에 대하여 결합특이성을 갖는 항체를 생성시키는 클론을 수득한 다음, 이클론을 배양하여 모노클로날 항체를 제조하고, 제조된 항체를 수거함을 특징으로하여, 췌장암 관련항원, 대장암 관련 항원 및 간암 관련 항원에 대해 결합 특이성을 갖는 모노클로날 항체를 제조하는 방법.
- 제1항에 있어서, 언급한 암 관련 항원이 췌장암 환자, 대장암 환자 및 간암 환자의 혈액 혈청중에 존재하고 ; 당 단백질을 함유하고 ; 분자량이 약 700,000 내지 1,500,000이고 ; 0.1M아세트산 완충용액 (pH4.5)중에 용해시킬 경우, 280nm에서 최대 흡광도를 가지며; COLO-201, TE-1, TE-2, TE-3, NRC-12,MKN-45 및 KATOⅢ으로 확정되는 세포의 배양된 상등 매질중에서 적어도 유리되는 항원인 방법
- 제1또는 2항에 있어서, 언급한 항체 형성 세포가 확정된 암세포라인 세포로부터 유도된 항원으로 면역화시킨 동물에게서 수득한 비장세포, 임파선 세포 또는 B림프구인 방법.
- 제3항에 있어서, 언급한 동물이 마우스, 쥐, 말, 염소 또는 토끼인 방법.
- 제1항에 있어서, 항체 형성 세포와 골수종 세포를 폴리에틸렌 글리콜 존재하에 30내지 40℃에서 약 1내지 3분동안 반응시키는 방법.
- 제1 또는 2항에 있어서, 수득한 클론을 BALB/C의 복강내에 이식시켜 배양하는 방법.
- 제1 또는 2항에 있어서, 수득한 클론을 1×10-4M하이 포크산틴, 4×1017M아미노프테린, 1.6×10-5M티미딘 및 10%송아지 태아혈청 또는 말의 혈청이 함유된 RPMI 매질 또는 D-MEM매질중에서 배양하는 방법.
- 혈구 또는 라텍스를 제1항의 제조방법에 의해 제조된 모노클로날 항체로 감작화시킴을 특징으로하여, 상기 모노클로날 항체를 응집소로서 함유하며 췌장암, 대장암 및 간암중의 하나 이상을 검사하기 위한 시약을 제조하는 방법.
- 제8항에 있어서, 언급한 혈구가 양, 닭 및 O형 인간의 적혈구인 방법.
- 제8항에 있어서, 언급한 혈구의 크기가 5내지 15μm인 방법.
- 제8항에 있어서, 언급한 혈구를 완충제 용액중에서 항체로 감작화시키는 방법.
- 제11항에 있어서, 적혈구 부유용액을 항체 함유 용액과 혼합하여 감작화시키는 방법.
- 제12항에 있어서, pH가 6.8내지 8.5이고 온도가 20내지 60℃인 상태에서 감작화시키는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP166980/84 | 1984-08-08 | ||
| JP59166980A JPS6144900A (ja) | 1984-08-08 | 1984-08-08 | モノクロ−ナル抗体及び癌抗原検出用試薬 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR860001593A true KR860001593A (ko) | 1986-03-20 |
Family
ID=15841169
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019850005719A KR860001593A (ko) | 1984-08-08 | 1985-08-08 | 모노클로날 항체의 제조방법 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US4959320A (ko) |
| EP (1) | EP0171083B1 (ko) |
| JP (1) | JPS6144900A (ko) |
| KR (1) | KR860001593A (ko) |
| CA (1) | CA1309039C (ko) |
| DE (1) | DE3580538D1 (ko) |
| ES (1) | ES8608685A1 (ko) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4701408A (en) * | 1984-01-30 | 1987-10-20 | Smithkline Beckman Corporation | Monoclonal antibody to activated macrophages |
| JPH0623763B2 (ja) * | 1985-02-15 | 1994-03-30 | 日清製粉株式会社 | モノクローナル抗体を含む肝疾患診断剤 |
| US4916055A (en) * | 1985-12-13 | 1990-04-10 | Baylor College Of Medicine | Detection of human cancer with a monoclonal antibody specific for antigen gp650 |
| JPH0673470B2 (ja) * | 1986-07-15 | 1994-09-21 | 協和醗酵工業株式会社 | 抗ヒト胃癌単クロ−ン性抗体ａｍｃ−４６２ |
| JPS6357596A (ja) * | 1986-08-29 | 1988-03-12 | Nisshin Flour Milling Co Ltd | モノクロ−ナル抗体、その製法およびそれからなる肝疾患診断剤 |
| EP0259773A3 (en) * | 1986-09-10 | 1990-05-30 | Asahi Kasei Kogyo Kabushiki Kaisha | A novel antibody specific for a cancer antigen |
| US4857452A (en) * | 1986-12-04 | 1989-08-15 | E. I. Du Pont De Nemours And Company | Assay for carcinoma of breast, colon and ovary |
| JPH0798000B2 (ja) * | 1987-05-23 | 1995-10-25 | 萩原 義秀 | ガン関連抗原特異的ヒト免疫グロブリン |
| DE3909799A1 (de) | 1989-03-24 | 1990-09-27 | Behringwerke Ag | Monoklonale antikoerper (mak) gegen tumorassoziierte antigene, ihre herstellung und verwendung |
| JPH06113831A (ja) * | 1992-04-08 | 1994-04-26 | Dev Center For Biotechnol | ハイブリドーマおよびその抗体 |
| US6225049B1 (en) | 1992-06-17 | 2001-05-01 | The United States Of America As Represented By The Department Of Health And Human Services | Human insulinoma-associated cDNA |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4471057A (en) * | 1981-06-30 | 1984-09-11 | The Wistar Institute | Detection of colorectal carcinoma |
| JPS59205327A (ja) * | 1983-03-11 | 1984-11-20 | スロ−ン−ケツタリング・インステイテユ−ト・フオ−・キヤンサ−・リサ−チ | ヒト膀胱及び尿管がんに対するモノクロ−ナル抗体及び方法 |
| US4683200A (en) * | 1984-05-17 | 1987-07-28 | Setsuo Hirohashi | Monoclonal antibody to human cancer antigen and method for producing same |
-
1984
- 1984-08-08 JP JP59166980A patent/JPS6144900A/ja active Granted
-
1985
- 1985-08-08 DE DE8585110006T patent/DE3580538D1/de not_active Expired - Lifetime
- 1985-08-08 CA CA000488311A patent/CA1309039C/en not_active Expired - Lifetime
- 1985-08-08 US US06/763,587 patent/US4959320A/en not_active Expired - Fee Related
- 1985-08-08 EP EP85110006A patent/EP0171083B1/en not_active Expired - Lifetime
- 1985-08-08 KR KR1019850005719A patent/KR860001593A/ko not_active Application Discontinuation
- 1985-08-08 ES ES546696A patent/ES8608685A1/es not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| EP0171083B1 (en) | 1990-11-14 |
| ES546696A0 (es) | 1986-07-16 |
| EP0171083A2 (en) | 1986-02-12 |
| CA1309039C (en) | 1992-10-20 |
| EP0171083A3 (en) | 1987-12-02 |
| US4959320A (en) | 1990-09-25 |
| JPS6144900A (ja) | 1986-03-04 |
| ES8608685A1 (es) | 1986-07-16 |
| DE3580538D1 (de) | 1990-12-20 |
| JPH0429358B2 (ko) | 1992-05-18 |
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| Date | Code | Title | Description |
|---|---|---|---|
| A201 | Request for examination | ||
| WITB | Written withdrawal of application |