KR850002325A - 과산화수소의 측정방법 및 시험조성물 - Google Patents

과산화수소의 측정방법 및 시험조성물 Download PDF

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KR850002325A
KR850002325A KR1019840001742A KR840001742A KR850002325A KR 850002325 A KR850002325 A KR 850002325A KR 1019840001742 A KR1019840001742 A KR 1019840001742A KR 840001742 A KR840001742 A KR 840001742A KR 850002325 A KR850002325 A KR 850002325A
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hydrogen peroxide
oxidase
measuring
reaction
alkyl
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KR1019840001742A
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KR900003541B1 (ko
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노리히또 아오야마 (외 3)
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와까기 시게도시
교오와 메덱스 가부시끼 가이샤
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

내용 없음

Description

과산화수소의 측정방법 및 시험조성물
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (11)

  1. 과산화 수소와, 다음 일반식
    이나 또는
    으로 표시되는 원색체와를 퍼록시다아제의 존재하에 반응시키고, 가시선 또는 적외선 근방에서 반응 용액의 흡광도를 측정하는 것으로 구성되는 것을 특징으로 하는 시료내의 과산화수소를 측정하는 방법.
    상기 식에서 Y는 수소 또는를 나타내고, Z는 산소원자나 유황원자를 나타내고, X는 수소, 알킬, 알케닐, 아릴, 아르알킬, 아미노 또는 치환된 마이노이고, R1은 하디르록실, 아미노 또는 치환된 아미노이고, R2는 수소, 하이드록실, 알킬, 알콕시, 아릴, 아르알킬, 알케닐, 아미노 또는 치환된 아미노이고, R3는 다음에 나타내는 일반식(Ⅲ),(Ⅳ),(Ⅴ)(Ⅵ)또는 (Ⅶ)로 표시되는 기이고, R4,R5또는 R5는 동일 또는 상이한 것으로서 수소, 알킬, 알케닐, 아실, 아릴, 아르알킬, 할로겐원자, 니트로, 설포, 카르복실, 하이드록실, 알콕시 또는 일반식(Ⅲ),(Ⅳ),(Ⅴ)(Ⅵ)또는 (Ⅶ)로 표시되는 기를 나타내며, J 는 －S－, －O－, 일반식또는 일반식이고, R7과 R8은 동일 또는 상이한 것으로 수소원자. 알킬 또는 알케닐이며 R5과 R6는 알케닐렌을 형성할 수 있다.
    상기식에서 A1은 알킬렌을 나타내고, A2는 R2의 의미와 같고, B1,B|2,B3,B4,B5또는 B6는 동일 또는 상이한 것으로 수소원자, 알킬, 알케닐, 아실, 아릴, 아르알킬, 할로겐원자, 니트로, 설포, 카르복실, 하이드록실 알콕시 또는 하이드록시 알킬을 나타낸다.
  2. 제1항에 있어서, 상기 과산화수소가 효소 반응으로 형성된 생성물인 것을 특징으로 하느 과산화수소의 측정방법.
  3. 제2항에 있어서, 효소반응 옥시다아제를 사용하는 기제의 산화반응인 것을 특징으로 하는 과산화수소의 측정방법.
  4. 제3항에 있어서, 상기 옥시다아제가 요산분해효소, 콜레스테롤 옥시다아제, 크산틴옥시다아제, 클린옥시다아제, 피루빈산염 옥시다아제, 글리세린－3－포스페이트 옥시다아제, 아실 Co A 옥시다아제, 글리세롤 옥시다아제 , 글루코오즈 옥시다아제 및 갈락토오즈 옥시다아제로 구성되는 군으로 부터 선택되는 것을 특징으로 하는 과산화수소의 측정방법.
  5. 제2항에 있어서, 상기의 과산화수소－생성반응과, 과산화수소와 원색체와의 반응이 동시에 수행되는 것을 특징으로 하는 과산화수소의 측정방법.
  6. 제1항에 있어서, 이 반응이 완충용액내에서 수행되는 것을 특징으로 하는 과산화수소의 측정방법.
  7. 제1항에서 정의한 원색체와 과산화효소로 구성되는 것을 특징으로 하는 과산화수소 측정용 시험조성물.
  8. 제7항에 있어서, 상기 조성물이 완충시약을 더 함유하는 것을 특징으로 하는 과산화수소의 측정방법.
  9. 제7항에 있어서, 상기 조성물이 계면활성제, 방부제 및 아스코르빈산염 옥시다아제로 구성되는 군으로부터 선택된 한 성분을 더욱 함유하는 것을 특징으로 하는 시험조성물.
  10. 제7항에 있어서, 상기 조성물이 효소성 과산화수소－생성계를 더 함유하는 것을 특징으로 하는 시험조성물.
  11. 제7항에 있어서, 상기 계가 옥시다아제를 함유하는 것을 특징으로 하는 시험조성물.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019840001742A 1983-03-31 1984-03-31 과산화수소의 측정방법 및 시험조성물 KR900003541B1 (ko)

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Application Number Priority Date Filing Date Title
JP58056652A JPS59182361A (ja) 1983-03-31 1983-03-31 過酸化水素の定量方法
JP58-56652 1983-03-31
JP56652 1988-03-10

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KR850002325A true KR850002325A (ko) 1985-05-10
KR900003541B1 KR900003541B1 (ko) 1990-05-21

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US (1) US4916058A (ko)
EP (1) EP0124287B1 (ko)
JP (1) JPS59182361A (ko)
KR (1) KR900003541B1 (ko)
DE (1) DE3486081T2 (ko)

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JPS62296A (ja) * 1985-06-26 1987-01-06 Kyowa Medetsukusu Kk 過酸化水素の定量方法
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JP2804079B2 (ja) * 1988-05-18 1998-09-24 協和メデックス株式会社 Ｎａｄ（ｐ）ｈの定量法
EP0361470B1 (en) * 1988-09-30 1993-12-22 Fujirebio Inc. Method for the chemiluminescence assay of the activity of peroxidase
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EP0714024B1 (en) * 1994-11-25 2002-01-30 Kyoto Dai-ichi Kagaku Co., Ltd. Method of and apparatus for determining hydrogen peroxide
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CN101595386B (zh) 2007-01-30 2012-05-09 爱科来株式会社 吩噻嗪衍生物色素的检测方法及用于该方法的显色剂试剂
EP2270105A4 (en) * 2008-03-19 2012-04-11 Arkray Inc STABILIZER FOR COLOR DEVELOPER AND ITS USE
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CN105699342B (zh) * 2014-11-28 2018-04-06 中国科学院大连化学物理研究所 一种利用滴定技术测量氧原子浓度的方法
FR3031593B1 (fr) * 2015-01-13 2019-05-03 Commissariat A L'energie Atomique Et Aux Energies Alternatives Analyse du phenol en phase liquide
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SE354470B (ko) * 1970-03-06 1973-03-12 Kabi Ab
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DE3526566A1 (de) * 1985-07-25 1987-02-05 Boehringer Mannheim Gmbh N-acyl-dihydroresorufin-derivate, verfahren zu deren herstellung sowie deren verwendung zur bestimmung von wasserstoffperoxid, peroxidatisch wirkender verbindungen oder peroxidase

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Publication number Publication date
JPH0427839B2 (ko) 1992-05-12
EP0124287A2 (en) 1984-11-07
KR900003541B1 (ko) 1990-05-21
US4916058A (en) 1990-04-10
EP0124287A3 (en) 1988-01-13
DE3486081D1 (de) 1993-04-08
DE3486081T2 (de) 1993-09-16
JPS59182361A (ja) 1984-10-17
EP0124287B1 (en) 1993-03-03

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