KR20240054455A - A Preparation Methods of a Cosmetic Composition Comprising Subcritical Water Extracts of Stevia Leaves - Google Patents

Abstract

The present invention is a process for producing a subcritical water extract of stevia leaves by adding 4,000 to 6,000 parts by weight of water based on 100 parts by weight of ground stevia leaves and extracting the extract for 10 to 20 minutes at a pressure of 5 to 9 MPa and a temperature range of 130 to 140 ° C. It provides a method for producing a cosmetic composition containing a stevia leaf subcritical water extract comprising the step.
The method for producing a cosmetic composition containing stevia leaf subcritical water extract according to the present invention provides anti-aging, anti-wrinkle, anti-inflammatory and/or whitening effects.

Description

{A Preparation Methods of a Cosmetic Composition Comprising Subcritical Water Extracts of Stevia Leaves}

The present invention relates to a method for producing a cosmetic composition containing a stevia leaf subcritical water extract, and more specifically, to a stevia leaf subcritical water extract for producing a cosmetic composition using an extract extracted from pulverized stevia leaves under subcritical conditions. It relates to a method of manufacturing a cosmetic composition comprising.

In an era where the average human lifespan can be expected to reach 100 years or longer due to advances in medicine and social, economic, and cultural developments, interest is focused on how to live younger and healthier regardless of gender and age. .

Reflecting these social trends, it is also a phenomenon of the times that issues regarding anti-aging are emerging in various fields such as diet, beauty, and medicine.

Meanwhile, the skin itself is an organ that not only protects the body from external irritants, but also plays an essential aesthetic and emotional role.

The skin is an exchange organ, and visual and tactile modalities play a very important role.

Additionally, skin contains various cell types, and melanocytes present in the basal layer of the epidermis are known to produce dark melanin pigment.

This melanin is sent to nearby keratin cells through small vesicles called melanosomes, causing tanning of the skin.

In addition, the skin is subject to external irritants such as ultraviolet radiation and pollution over time, and its appearance is prone to change.

In particular, solar radiation, specifically ultraviolet radiation, is known to cause melanin synthesis, resulting in "darkening" or pigmentation of the skin.

For decades in the West, a tanned skin tone and/or the "radiant" appearance of tanned skin has been perceived by both men and women as a sign of good health and/or attractiveness.

Therefore, there are cases where UV tanning beds at certain stores continue to tan throughout the year, which can easily cause skin aging and/or the formation of skin spots due to increased pigmentation in the skin, which causes irregular pigmentation. Spots may occur during or after sun exposure, causing irregular pigmentation of the skin, creating an unattractive appearance.

Additionally, hyperpigmentation of the skin may occur due to photosensitivity and/or healing wounds, which may also result in an unattractive appearance.

Moreover, hormonal changes in pregnant women, especially increased levels of female sex hormones, greatly increase melanin synthesis by melanocytes. In these cases, pregnancy hyperpigmentation or melasma is caused by increased production of sex hormones by melanocytes. It is known to be associated with hyperactivity.

Exposure of the skin to the sun, especially during pregnancy, can cause hyperpigmentation of the skin, such as spots called the "mask of pregnancy," which may appear over time, during pregnancy, and after birth. It may remain even after female sex hormones return to normal levels, or may decrease over time, but naturally reappears whenever exposed to the sun/ultraviolet rays, which can cause irregular pigmentation on facial skin.

These pigment spots can also occur in older people, such as senile lentigo or age spots, and this irregularity in pigmentation occurs due to the localized concentration of melanin on the skin surface.

Therefore, it is becoming important to develop compositions or products that can treat defects in skin deposition, i.e. increased melanin production due to hormonal changes, excessive exposure to ultraviolet rays and/or the sun, or skin aging, regardless of the cause. .

Meanwhile, in Asia, especially China, Japan, and Korea, skin appearance is considered a socioeconomic indicator. In fact, in this region, people with tanned skin may be perceived as having relatively inferior social occupations, such as people working on construction sites or outdoors, or farmers. In other words, lifestyle determines the amount of sun exposure and can be considered a reflection of socioeconomic level.

Additionally, for centuries in Asia, white skin was perceived as beauty and/or belonging to a wealthy social class, for example, in the case of geishas in Japan. Ultimately, wrinkles, fine lines, and pigment spots are considered to be one of the major clinical signs of skin aging for Asian skin.

Therefore, it is necessary to develop a composition or product that enables skin glow by reducing melanin production or promoting its decomposition.

Meanwhile, as a prior art, Korean Patent Publication No. 10-2016-0120355 discloses a cosmetic or dermatological composition containing meadowsweet extract, one or more mulberry leaf extracts, and salicylic acid.

Republic of Korea Public Patent No. 10-2016-0120355 (publication date 2016.10.17)

The present invention was created to overcome the above-mentioned problems, and by constructing a cosmetic composition using an extract extracted from pulverized stevia leaves under subcritical conditions, it has anti-aging, anti-wrinkle, anti-inflammatory and/or whitening effects. To provide effects such as:

The present invention is a process for producing a subcritical water extract of stevia leaves by adding 4,000 to 6,000 parts by weight of water based on 100 parts by weight of ground stevia leaves and extracting the extract for 10 to 20 minutes at a pressure of 5 to 9 MPa and a temperature range of 130 to 140 ° C. It provides a method for producing a cosmetic composition containing a stevia leaf subcritical water extract comprising the step.

The method for producing a cosmetic composition containing stevia leaf subcritical water extract according to the present invention provides anti-aging, anti-wrinkle, anti-inflammatory, and/or whitening effects.

Figure 1 is a diagram showing the anti-inflammatory activity of the stevia leaf subcritical water extract according to the present invention.

Hereinafter, the present invention will be described in detail.

The present invention is a process for producing a subcritical water extract of stevia leaves by adding 4,000 to 6,000 parts by weight of water based on 100 parts by weight of ground stevia leaves and extracting the extract for 10 to 20 minutes at a pressure of 5 to 9 MPa and a temperature range of 130 to 140 ° C. It provides a method for producing a cosmetic composition containing a stevia leaf subcritical water extract comprising the step.

In addition, the present invention adds 4,000 to 6,000 parts by weight of water based on 100 parts by weight of purified water and 100 parts by weight of ground stevia leaves, and extracts the extract for 10 to 20 minutes at a pressure of 5 to 9 MPa and a temperature range of 130 to 140 ° C. 1 to 5 parts by weight of stevia leaf subcritical water extract; and a cosmetic composition mixing step of mixing 5 to 40 parts by weight of vegetable oil. It provides a method for producing a cosmetic composition containing a stevia leaf subcritical water extract.

In the present invention, a cosmetic composition refers to a composition used on the human body to clean and/or beautify the human body to add attractiveness, brighten the appearance, or maintain and/or promote the health of the skin, etc., and includes detergents (detergents) and cosmetics ( cosmetics), etc.

In the present invention, a supercritical fluid is when a substance is under a temperature and pressure higher than its critical point, that is, the temperature and vapor pressure at the critical state where the two states of liquid and gas become indistinguishable from each other. , refers to a fluid in a state above the supercritical point.

Additionally, in the present invention, sub-supercritical fluid refers to a fluid that has almost similar characteristics to a supercritical fluid.

Additionally, in the present invention, the subcritical extraction method refers to an extraction method using the subcritical fluid.

At this time, the subcritical fluid may be water, carbon dioxide, ethylene, ethane, propane, acetylene, or ammonia, and is preferably economical, free of harmful components, and can be safely disposed of, so it is not only environmentally friendly, but also improves extraction efficiency according to the present invention. It may be water for which conditions for maximizing have been confirmed, more preferably subcritical water. Accordingly, the subcritical water refers to water under subcritical conditions.

The method for producing a cosmetic composition according to the present invention is to add 4,000 to 6,000 parts by weight of water, preferably about 5,000 parts by weight, based on 100 parts by weight of stevia leaves, specifically pulverized stevia leaves, and add 5 to 9 MPa, preferably about 5,000 parts by weight. and a stevia leaf subcritical water extract extracted at a pressure of 7 MPa and a temperature range of 130 to 140° C., preferably about 135° C., for 10 to 20 minutes, preferably about 15 minutes.

Here, the water may be general water or distilled water, and the distilled water may be primary distilled water, secondary distilled water, or tertiary distilled water.

Stevia (Stevia rebaudiana) according to the present invention is used as a diet sweetener because its leaves and stems contain low-calorie sweet ingredients, and it contains a component called stevioside, which is 200 to 300 times more sweet than sugar. It has a sweet taste, has very low calories (4 calories per gram), and is a non-toxic natural sweetener, making it a necessity for diabetics and modern people on diet.

Moreover, the stevioside has been reported to have effects on controlling insulin-sensitive and insulin-resistant diabetes in mice, and diterpene compounds have recently been confirmed to have antioxidant, antibacterial, and antiviral effects, and have been identified as inflammatory agents with a new mechanism. .

In particular, stevioside is highly soluble in water or alcohol, has heat resistance, and is non-toxic, so it is used as a sweetener for chaverts, chewing gum, soft drinks, and medicine.

Stevia, specifically stevia leaves, can provide anti-aging, anti-wrinkle, anti-inflammatory, antioxidant and/or whitening effects when prepared in extract form and then used in cosmetic compositions.

The stevia leaves used to prepare the stevia leaf extract may be any stevia leaves commonly used in the art, but are preferably pulverized stevia leaves, preferably those with an average particle diameter of 1 to 10 mm. It is better to use ground powder.

Here, the production of the pulverized stevia leaves can be performed using a typical land plant pulverizer, and a drying process of the stevia leaves can be additionally performed prior to the pulverized product production process.

In this respect, producing the pulverized stevia leaves may be performed by a method including drying the stevia leaves and pulverizing the dried stevia leaves.

The drying of the stevia leaves can be carried out by a conventional plant or land plant drying method, in one embodiment, a natural drying method, a hot air drying method, or a freeze drying method, and the natural drying method is performed under temperature conditions of 20°C to 30°C and ventilation. It can be performed under possible ventilation conditions.

Meanwhile, the stevia leaf extract according to the present invention can be used as an extract extracted by methods such as hot water extraction and subcritical water extraction. However, in terms of the extraction yield of the stevia leaf extract, it is difficult to prepare the stevia leaf extract using the subcritical water extraction method. It is very effective.

Accordingly, the stevia leaf extract according to the present invention is prepared by adding 4,000 to 6,000 parts by weight of water, preferably about 5,000 parts by weight, based on 100 parts by weight of pulverized stevia leaves, and applying pressure of 5 to 9 MPa, preferably about 7 MPa, and 130 It may be a stevia leaf subcritical water extract characterized by extraction for 10 to 20 minutes, preferably about 15 minutes, in a temperature range of from 140°C to 140°C, preferably about 135°C.

Here, the subcritical water extract may optionally be subjected to a filtration process, concentration process, or drying process after the subcritical extraction. The filtration process may be performed using filter paper, and the concentration process may be performed through reduced pressure concentration, etc. The drying process can be performed by methods such as hot air drying or freeze drying.

Therefore, the cosmetic composition according to the present invention may be a cosmetic composition for whitening, a cosmetic composition for wrinkle improvement, a cosmetic composition for preventing or improving wrinkles, or a cosmetic composition for anti-wrinkle.

The cosmetic composition preferably contains 0.001 to 40% by weight of stevia leaf subcritical water extract based on the total composition. In other words, the content of stevia leaf subcritical water extract must exceed the minimum level to achieve skin-related functionality, specifically anti-wrinkle, whitening, and/or anti-skin aging effects. In consideration of applicability to various dosage forms, it can be freely adjusted within the above range. At this time, the content of the active ingredient and the content of additional ingredients can be appropriately adjusted within the above range depending on the content of the ingredients contained in the formulation and/or cosmetic composition.

As such an example, one aspect of the method for producing a cosmetic composition containing a stevia leaf subcritical water extract according to the present invention is 4,000 to 6,000 parts by weight of water, preferably based on 100 parts by weight of purified water and 100 parts by weight of pulverized stevia leaves. Add about 5,000 parts by weight of stevia leaves and extract them for 10 to 20 minutes, preferably about 15 minutes, at a pressure of 5 to 9 MPa, preferably about 7 MPa, and a temperature range of 130 to 140°C, preferably about 135°C. 1 to 5 parts by weight of subcritical water extract; And it may include a cosmetic composition mixing step of mixing 5 to 40 parts by weight of vegetable oil.

The purified water according to the present invention may be any conventional purified water in the art, but it is preferably used to prepare cosmetic compositions that are harmless to the human body.

In addition, the remaining ingredients other than purified water used in the cosmetic composition manufacturing method are based on 100 parts by weight of the purified water.

Vegetable oils according to the present invention include vegetable oils commonly used in the art, such as sunflower oil, coconut oil, castor oil, lavender oil, avocado oil, canola oil, grape seed oil, or mixtures thereof or various other oils. Oils can be mixed and used, and the amount used is preferably 5 to 40 parts by weight based on 100 parts by weight of purified water.

At this time, the vegetable oil preferably contains more than 1.5% by weight and 0.5% by weight of sunflower oil and coconut oil based on 100% by weight of the total vegetable oil usage.

Meanwhile, in the method of manufacturing a cosmetic composition according to the present invention, specifically the method of manufacturing a cosmetic composition comprising a stevia leaf subcritical water extract, the cosmetic composition may be any cosmetic composition commonly used in the art, but is preferred. It can be provided for purposes such as skin ointment, basic cosmetics, makeup cosmetics, body cosmetics, or shaving cosmetics. Examples of basic cosmetics include essence, lotion, cream, gel, lotion, pack, massage cream, emulsion, etc. Examples of the makeup cosmetics may include foundation, lipstick, eye shade, eyeliner, mascara, eyebrow pencil, makeup base, etc., and body cosmetics may include soap, liquid detergent, bath salts, and sunscreen cream. , sun oil, etc., and cosmetics for shaving include aftershave lotion, shaving cream, etc.

In addition, the cosmetic composition according to the present invention can be used in other cosmetic compositions, specifically skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, It may be a cosmetic composition mixed with cosmetic compositions such as essence, nutritional essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder, and eye shadow. Depending on the condition, it may be diluted and used.

In addition, the cosmetic composition of the present invention, in addition to containing stevia leaf subcritical water extract as an active ingredient, additionally contains functional substances with anti-wrinkle or whitening effects or other functional substances with UV blocking or moisturizing effects. Substances such as excipients and diluents to improve the material or physical properties may be additionally included.

In one embodiment, fragrances, pigments, disinfectants, antioxidants, preservatives, moisturizers, thickeners, inorganic salts, or synthetic polymer materials may be additionally added to the cosmetic composition to improve physical properties. Other ingredients that may be added may include fat ingredients, emollients, surfactants, organic pigments, inorganic pigments, organic powders, ultraviolet absorbers, pH adjusters, alcohol, blood circulation promoters, cooling agents, antiperspirants, or purified water.

In addition, the cosmetic composition may further contain one or more selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extract.

Components that may be added other than the above-mentioned active ingredients are not limited to this, and any of the above-mentioned components can be mixed within the range that does not impair the purpose and effect of the present invention.

The method for producing a cosmetic composition according to the present invention, specifically the method for producing a cosmetic composition containing a stevia leaf subcritical water extract, may include preparing a cosmetic composition by further mixing one or more types of adjuncts of the following specific embodiments. there is.

In a specific embodiment, the method for manufacturing a cosmetic composition according to the present invention, specifically the method for manufacturing a cosmetic composition comprising a stevia leaf subcritical water extract, is used to improve blood flow and improve lipid metabolism, such as anti-cancer, anti-cholesterol, blood coagulation inhibition, and blood pressure control. It may include further mixing 1 to 3 parts by weight of seaweed extract based on 100 parts by weight of purified water.

Here, the seaweed is a brown algae belonging to the seaweed family of the kelp order, distributed along the entire coast of Korea, and is a seaweed native to the Far East, such as the Maritime Province, China, and Japan, and contains minerals such as sodium, potassium, calcium, magnesium, phosphorus, and sulfur, dietary fiber, It is rich in bioactive substances such as linoleic acid and vitamins.

In particular, research is being actively conducted on seaweed for use as a variety of cosmetic materials due to its excellent moisturizing, antioxidant, and anti-inflammatory properties.

In another specific embodiment, the method for manufacturing a cosmetic composition according to the present invention, specifically the method for manufacturing a cosmetic composition comprising a stevia leaf subcritical water extract, is prepared by adding 100% purified water to add various physiological activities such as antioxidant activity, detoxification activity, and anti-inflammatory activity. It may include further mixing 1 to 3 parts by weight of Houttuynia cordata extract based on parts by weight.

Here, Houttuynia cordata is a wild perennial herb belonging to the Tribaekaceae family and is also called Yakmomil or Sambaekcho, and its origin is Korea, China, Japan, etc. Houttuynia cordata is named so because its leaves emit a unique fishy smell. It contains various physiologically active substances and has long been used as a herbal medicine with pharmacological effects such as diuresis, pain relief, and hemostasis.

In another specific embodiment, the skin glowing cosmetic composition according to the present invention may further include mixing 1 to 3 parts by weight of golden hazelnut extract based on 100 parts by weight of purified water to add vitamin C and flavonoids.

Here, the Golden Hagia tangerine is an evergreen tree belonging to the Rutaceae family and is distributed in Jeju. The sugar content is high, and the flesh is soft and juicy. It grows up to 3m in height, and the outer ears are oval-shaped and the edges are plain without teeth. Red tangerines contain a lot of vitamin C and flavonoids.

In another specific embodiment, the cosmetic composition according to the present invention, specifically the skin glowing cosmetic composition, may further include mixing 1 to 3 parts by weight of camphor extract based on 100 parts by weight of purified water to provide a strong antioxidant effect. .

Here, the camphor tree is an evergreen broad-leaved tree belonging to the Magnolia family, Lauraceae, and is distributed in Vietnam, Taiwan, Japan, etc. and grows naturally in Jeju Island. It is about 20 m tall and its young branches are yellow-green. The leaves are alternate and shiny, with three leaf veins running side by side, and young leaves are slightly red. Camphor, which is made by distilling wood, branches, leaves, and roots in steam, is used as a tonic or stimulant, and is made into an injectable medicine and used as a stimulant for the respiratory center, blood vessels, nerves, central nervous system, and heart. It is also used as an ingredient in insect repellent, preservatives, and fragrances, and its wood is used as building material and furniture material.

In another specific embodiment, the method for manufacturing a cosmetic composition according to the present invention may further include mixing 1 to 3 parts by weight of green tea extract based on 100 parts by weight of purified water to improve skin functionality due to aging.

Here, the green tea has a detoxifying and sterilizing effect due to tannin components, a strong deodorizing effect against odor components due to flavonoid components, an antibacterial effect, etc., and provides improvement in skin functionality due to aging.

In another specific embodiment, the method for producing a cosmetic composition according to the present invention may include further mixing 1 to 3 parts by weight of broccoli extract based on 100 parts by weight of purified water to improve skin resistance to harmful substances.

The broccoli (Brassica oleracea var. Italica (Broccoli)) is a plant belonging to the cruciferous family and contains a large amount of beta-carotene, rutin, ascorbic acid, selenium, quercetin, glutathione, etc., which have antioxidant properties, calcium, vitamin C, Contains nutrients such as folic acid. Broccoli increases collagen synthesis in the skin, prevents skin aging, is involved in improving skin resistance against harmful substances, and has the effect of adding tension to the skin and helping with pore convergence.

In another specific embodiment, the method for manufacturing a cosmetic composition according to the present invention further comprises mixing 1 to 3 parts by weight of aloe extract based on 100 parts by weight of purified water in order to improve and manage healthy skin by making the skin soft and flexible and providing conditioning. may include

In another specific embodiment, the method for manufacturing a cosmetic composition according to the present invention may include further mixing 1 to 5 parts by weight of glycerin based on 100 parts by weight of purified water to improve skin moisturizing ability.

In another specific embodiment, the method for manufacturing a cosmetic composition according to the present invention is to add 1 to 3 parts by weight of persimmon fermentation extract stock solution based on 100 parts by weight of purified water in order to add various physiological effects such as anti-cancer, blood pressure lowering, antibacterial, and anti-inflammatory. Further mixing may be included.

Here, the raw fermentation extract solution is mixed with 5 to 50 parts by weight of oligosaccharides, 5 to 50 parts by weight of sugar, and 5 to 15 parts by weight of fermentation microorganisms, based on 100 parts by weight of permeation, and incubated for 50 to 70 days at a temperature range of 35 to 45 ° C. This includes fermenting and maturing for a period of time to produce a fermented fermented broth and then extracting it using a hot water extraction method.

In another specific embodiment, the method for manufacturing a cosmetic composition according to the present invention may include further mixing 1 to 3 parts by weight of pine needle fermentation extract stock solution based on 100 parts by weight of purified water to prevent skin irritation.

Here, the pine needle fermentation extract stock solution is mixed with 5 to 50 parts by weight of oligosaccharides, 5 to 50 parts by weight of sugar, and 5 to 15 parts by weight of fermentation microorganisms, based on 100 parts by weight of pine needles, and incubated at a temperature range of 35 to 45 ° C. for 50 to 70 days. This includes producing a fermented pine needle liquid by fermenting and maturing it for a while and then extracting it using a hot water extraction method.

Meanwhile, the pine needles, which are the leaves and shoots of pine trees, contain ascorbic acid, vitamins A, B, K, flavonoids, and anthocyanins, and are known to be good for high blood pressure, stroke, diabetes, anti-aging, anemia, and hangover relief. .

In another specific embodiment, the method for manufacturing a cosmetic composition according to the present invention may include further mixing 1 to 3 parts by weight of Angelica root fermentation extract stock solution based on 100 parts by weight of purified water to improve blood circulation in the skin.

Here, the Angelica root fermentation extract crude solution is mixed with 5 to 50 parts by weight of oligosaccharides, 5 to 50 parts by weight of sugar, and 5 to 15 parts by weight of fermentation microorganisms, based on 100 parts by weight of Angelica root, and incubated for 50 to 70 days at a temperature range of 35 to 45 ° C. This includes producing fermented Angelica root broth by fermenting and maturing it for a while and then extracting it using a hot water extraction method.

In another specific embodiment, the method for producing a cosmetic composition according to the present invention may include further mixing 1 to 3 parts by weight of fermented sophora extract stock solution based on 100 parts by weight of purified water.

The soybean fermented extract stock solution is mixed with 5 to 50 parts by weight of oligosaccharides, 5 to 50 parts by weight of sugar, and 5 to 15 parts by weight of fermentation microorganisms, based on 100 parts by weight of soybean ginseng, and fermented for 50 to 70 days at a temperature range of 35 to 45 ° C. This includes producing a fermented soybean extract by maturing it and then extracting it using a hot water extraction method.

Here, Sophora angustifolia SIEB. et Zucc is a perennial herb of the legume family that reaches 1 m in height when fully grown. The shape of the leaves is similar to that of the locust tree (Goehwa), so it is nicknamed Jigoe, Sugoe, and Yagoe. The roots are dug up in the fall, washed and dried. Although it tastes bitter, it is called Gosam because it has the same effect as ginseng. The components of Sophora ginseng include alkaloids found throughout the plant. The total alkaloid of the root is 1.1%, and the alkaloid components include matrine (sophocarpidine), oxymatrine, sophoranol, anagyrine, methylcytizine, babutyfline, sophocarpine, and allomatrine. In addition, it contains about 13% sugar, and flavonol compounds such as isoanhydroicaridin and noranhydroicaridin were isolated from the root.

In another specific embodiment, the method for manufacturing a cosmetic composition according to the present invention may include further mixing 1 to 3 parts by weight of wintergreen fermentation extract stock solution based on 100 parts by weight of purified water.

The wintergreen fermentation extract stock solution is mixed with 5 to 50 parts by weight of oligosaccharides, 5 to 50 parts by weight of sugar, and 5 to 15 parts by weight of fermentation microorganisms, based on 100 parts by weight of wintergreen, and fermented for 50 to 70 days at a temperature range of 35 to 45 ° C. This includes producing a bittergreen fermented broth by aging it and then extracting it using a hot water extraction method.

Here, the wintergreen (leaf) usually has warm and sweet sensory characteristics (scent and taste), and its extract is used in perfume, food, beverages, candy, chewing gum, etc., and the decoction is used as a gargle to soothe sore throat. It was also used to treat skin inflammation.

Meanwhile, the fermentation microorganism used to produce the above-mentioned fermentation extract stock solution may be any fermentation microorganism common in the art, but is preferably Bacillus or Micrococcus conglomeratus. ), Micrococcus epimetheus, Micrococcus subflavescens, Micrococcus varianus, Lactic acid bacteria, Butyric acid bacteria ), Leuconostoc mesenteroides, Streptococcus faecalis, Rhizopus tritici, Rhizopus tamari, Rhizopus chinensis, Rhizopus arrhizus, Rhizopus oryzae, Rhizopus delemar, Rhizopus peka, Rhizopus nodosus, Monascus purupureus ( Monascus purpureus), Penicillium glaucum, Mucor mucedo, Mucor plumbeus, Mucorracemosus, and Mucor javanicus. (Fungi) or at least one mixture selected from these.

In another specific embodiment, the method for producing a cosmetic composition according to the present invention may include further mixing 1 to 5 parts by weight of propylene glycol based on 100 parts by weight of purified water to improve moisturizing performance.

Here, propylene glycol is an aliphatic alcohol that helps dissolve the composition and is a moisture carrier widely used in the cosmetics field. It has higher skin absorption and less stickiness than glycerin.

In particular, the propylene glycol protects the composition from melting at high temperatures or freezing in the cold, and can help other active ingredients penetrate into the skin.

In another specific embodiment, the method for producing a cosmetic composition according to the present invention may further include 1 to 3 parts by weight of fragrance based on 100 parts by weight of purified water.

Here, the perfume may be any conventional perfume in the art, but it is recommended to use herbal oil, more preferably rosemary, oregano, bergamot, yarrow, basil, mint, sage or lemon balm, jesmine, It is recommended to use herbal oils such as borage, arnica, spatium, clary sage, and peppermint.

In another specific embodiment, the method for producing a cosmetic composition according to the present invention may include further mixing 1 to 3 parts by weight of fluconazole based on 100 parts by weight of purified water to improve the antifungal effect.

In another specific embodiment, the method for manufacturing a cosmetic composition according to the present invention may further include 1 to 3 parts by weight of tocopheryl acetate based on 100 parts by weight of purified water to protect the skin from harmful oxygen.

In another specific embodiment, the method for producing a cosmetic composition according to the present invention may further include 1 to 3 parts by weight of sorbitan sesquioleate based on 100 parts by weight of purified water in order to improve the emulsibility of the composition. .

In another specific embodiment, the method for producing a cosmetic composition according to the present invention may further include 1 to 3 parts by weight of copper tripeptide-1 based on 100 parts by weight of purified water for skin conditioning.

In another specific embodiment, the method for producing a cosmetic composition according to the present invention may further include 1 to 3 parts by weight of snake venom peptide (dipeptide diaminobutyroyl) based on 100 parts by weight of purified water for wrinkle improvement, etc.

In another specific embodiment, the method for manufacturing a cosmetic composition according to the present invention adsorbs and removes dust, fine minerals, etc. settled on the skin and improves the usability and whitening function of the cosmetic by adding the illite eluate to 1 part by weight based on 100 parts by weight of purified water. It may further include from 5 to 5 parts by weight.

As mentioned above, illite is a mineral of the same series as hundreds of mica rich in SiO ₂ , MgO, H ₂ O, hardness 1-2, specific gravity 2.6-2.9, produced in aluminum-rich heterogeneous or tuff-like sedimentary rocks, and hydrothermal mineral deposits. It is calculated as an altered mineral and is widely known to have the effect of adsorbing heavy metals, deodorizing, far-infrared rays, and releasing negative ions. Therefore, it is more effective to use an eluate of illite from which the active ingredient is extracted rather than using illite directly. Light eluate can be prepared through the following process.

The method for producing illite eluate according to the present invention consists of a grinding step, an elution step, and a condensation step.

In the pulverizing step, the illite mineral is pulverized to enable better extraction of the active ingredients of the illite in the elution step. For this purpose, the appropriate size of the pulverized illite is 0.1-0.5 mm. If the size of illite is less than 0.1mm, there is a problem that the burden of grinding costs increases, and if it exceeds 0.5mm, there is a problem of poor elution due to the relatively small surface area in contact with the solvent. Grinding of illite is similar to that of conventional minerals. You can use the grinding method.

The elution step is a step of eluting the active ingredient of illite mineral and is as follows. In the pulverizing step, the pulverized illite and purified water are placed in a heating furnace and heat is applied to elute the active ingredients from the pulverized illite mineral.

Here, it is desirable to maintain the heating furnace temperature at 185 to 400°C during elution. 185°C is the temperature at which the mixture of illite and purified water begins to boil. Below 185°C, the extracted minerals are hardly extracted. , exceeding 400℃ is not good because there is a problem of illite being denatured.

The condensation step collects the illite eluate eluted in vapor form in the elution step, cools it to room temperature, and condenses it. The progress time is not particularly limited, but is preferably set to 30 minutes to 2 hours in consideration of work time efficiency. .

In another specific embodiment, the method for producing a cosmetic composition according to the present invention may further include 1 to 5 parts by weight of black bean extract based on 100 parts by weight of purified water in order to improve skin elasticity and antioxidant properties, improve skin beauty, and prevent skin aging. .

Black beans are rich in anthocyanin pigments, proteins, and unsaturated fatty acids, and are widely known to be good for improving skin elasticity and antioxidant properties, improving skin beauty, and preventing skin aging. These black beans are effective in producing anthocyanins, etc., when extracted only through conventional hot water extraction. Since the ingredients are not properly extracted, the present invention can obtain black bean extract through the following process.

The present invention includes a steaming process of steaming black beans at a temperature of 100 to 130° C. and a pressure of 2 to 4 kgf/cm ² for 2 to 5 minutes; A roasting process of roasting the steamed black beans at a temperature of 200 to 400° C. for 40 to 150 seconds; A cold extraction process of cold extracting the roasted black beans with 5 to 20 times the weight of water at 20 to 30° C. for 60 to 100 minutes; After the cold extraction is completed, a hot water extraction process of hot water extracting the black bean solids with 20 to 30 times the weight of water at a temperature of 80 to 95 ° C. for 5 to 20 minutes; And, it includes a black bean extract producing process of mixing the cold extract on which the cold extraction has been completed and the hot water extract on which the hot water extraction has been completed.

Here, through the steaming process for 2 to 5 minutes at a temperature of 100 to 130 ℃ and a pressure of 2 to 4 kgf / cm ² , the unsaturated fat contained in the black beans themselves is released to coat the surface of the black beans, and during roasting, the unsaturated fat contained in the black beans is released. Deterioration and discoloration of the anthocyanin contained can be prevented. If the pressure is less than 2 kgf/cm ² , the unsaturated fat contained in the black beans themselves may not be released properly, and if the pressure exceeds 4 kgf/cm ² , the unsaturated fat may be excessive. There is a problem of release, and if the time is less than 2 minutes, gelatinization is insufficient and the texture is hard, and if the time is more than 5 minutes, the texture becomes too soft and mushy, there is a sour smell and problems with contents eluting, and the roasting process It can prevent raw odor, discoloration, etc. in the above temperature and time range, and the cold extraction is a method of extracting the extract by slowly flowing roasted black beans with cold water at 20 to 30 ° C. This is the optimal condition for obtaining solid content, and has the advantage of extracting active ingredients while maintaining freshness that cannot be obtained from conventional hot water extraction. The hot water extraction process extracts more anthocyanins from black bean solid content in the above temperature and time range. can do.

In another specific embodiment, the method for manufacturing a cosmetic composition according to the present invention may further include 1 to 5 parts by weight of Baekbokryeong extract based on 100 parts by weight of purified water to improve skin beauty and anti-inflammation.

The Baekbokryeong is a white-colored, fungus of the fungi family, which is good for skin beauty by moderately controlling sweat and has the effect of suppressing dermatitis. The Baekbokryeong contains beta-pachyman and , It contains a large number of proteins, glucose, etc., and is known to be good for strengthening blood sugar and suppressing contact dermatitis. The manufacturing process is as follows.

First, wash the Baekbokryeong thoroughly with water. Then, place the Baekbokryeong in a double boiler and heat in a double boiler at a temperature of 80℃ for 18 hours. Next, the leachate is placed in an extractor and extracted by heating for 12 hours under a temperature range of 150°C and a pressure of 4kgf/cm ² .

Here, washing Baekbokryeong with water is to remove foreign substances, and preparing Baekmokryeong extract by boiling and extracting Baekbokryeong is to concentrate and extract the active ingredients of Baekbokryeong.

In another specific embodiment, the cosmetic composition manufacturing method according to the present invention may further include 1 to 5 parts by weight of para-amino benzoic acid based on 100 parts by weight of purified water in order to protect the skin by absorbing ultraviolet rays.

In another specific embodiment, the method for producing a cosmetic composition according to the present invention may further include 1 to 5 parts by weight of hyaluronic acid based on 100 parts by weight of purified water in order to improve the skin moisturizing effect, wherein the hyaluronic acid is a biological component. , The skin moisturizing effect is very excellent.

In another specific embodiment, the method for producing a cosmetic composition according to the present invention may further include 1 to 3 parts by weight of adenosine based on 100 parts by weight of purified water to revitalize the skin, increase skin elasticity, and improve skin wrinkles. Adenosine promotes DNA synthesis in skin fibroblasts, increases protein synthesis, and increases cell size. It increases nucleic acid synthesis and activates protein metabolism to maintain the tonic properties of skin cells that die or have functional decline. It is known to help improve cell function, expand cell size, and improve wrinkles caused by destruction of dermal cells and aging.

In another specific embodiment, the method for manufacturing a cosmetic composition according to the present invention reduces sebum secretion, maintains the balance of oil produced by the sebaceous glands of the skin, soothes irritated skin, softens skin texture, and reduces hyperpigmentation. For this purpose, niacinamide may be further included in an amount of 0.1 to 2 parts by weight based on 100 parts by weight of purified water. The niacinamide is a structure in which an amide group is attached to niacin and is an element that constitutes water-soluble vitamin B3, Niacinamide is known as a raw material that inhibits melanin pigmentation by preventing the transfer of melanin pigment produced in basal layer melanocytes, the lowest layer of the epidermis, to keratinocytes (keratinocytes).

In another specific embodiment, the method for manufacturing a cosmetic composition according to the present invention increases the water-binding ability of the skin to make the stratum corneum flexible and inhibits melanin synthesis in the skin by adding 0.1 to 2 parts by weight of lactic acid based on 100 parts by weight of purified water. It may further include the lactic acid, which is lactic acid extracted from the fermentation process of milk and is an organic compound having an asymmetric carbon atom in which four atom groups of carboxyl group, hydroxyl group, methyl group, and hydrogen are combined, and is a spoilage in the early stage of liquor fermentation. It is also used as a deterrent.

Hereinafter, the present invention will be described in detail through examples. However, the following examples are only for illustrating the present invention in detail and do not limit the scope of the present invention by these examples.

[Example 1]

A cosmetic composition was prepared by mixing 100 g of crushed stevia leaves with 5,000 g of water and extracting the mixture for about 15 minutes at a temperature of about 7 MPa and about 135° C. to obtain a subcritical water extract of stevia leaves.

[Example 2]

100 g of ground stevia leaves and 5,000 g of water were mixed and extracted at a temperature of about 7 MPa and about 135°C for about 15 minutes to extract a stevia leaf subcritical water extract, followed by 100 g of purified water, 3 g of the extracted stevia leaf subcritical water extract, and A cosmetic composition was prepared by mixing 25g of vegetable oil consisting of 7.5g of sunflower oil, 3.75g of coconut oil, and 13.75g of castor oil.

[Example 3]

The same method as Example 2 was carried out, but 2 g of seaweed extract was further mixed.

[Example 4]

The same method as Example 2 was performed, except that 2 g of Houttuynia cordata extract was further mixed.

[Example 5]

The same method as Example 2 was performed, but 2 g of golden hazelnut extract was additionally mixed.

[Example 6]

The same method as Example 2 was performed, but 2 g of camphor extract was added.

[Example 7]

The same method as Example 2 was performed, but 2 g of green tea extract was added.

[Example 8]

The same method as Example 2 was performed, but 2 g of broccoli extract was added.

[Example 9]

The same method as Example 2 was performed, except that 2 g of aloe extract was added.

[Example 10]

This was carried out in the same manner as in Example 2, except that 3 g of glycerin was added.

[Example 11]

This was carried out in the same manner as in Example 2, except that 2 g of raw fermentation extract was added to the mixture.

[Example 12]

This was carried out in the same manner as in Example 2, except that 2 g of pine needle fermentation extract stock solution was further mixed.

[Example 13]

The same method as Example 2 was performed, except that 2 g of Angelica root fermented extract stock solution was further mixed.

[Example 14]

The same method as Example 2 was carried out, except that 2 g of fermented soybean extract stock solution was further mixed.

[Example 15]

The same method as Example 2 was performed, except that 2 g of wintergreen fermented extract stock solution was further mixed.

[Example 16]

The same method as Example 2 was carried out, except that 3 g of propylene glycol was further mixed.

[Example 17]

The same method as Example 2 was performed, but 2 g of rosemary oil was added.

[Example 18]

The same method as Example 2 was performed, but 2 g of fluconazole was added.

[Example 19]

The same method as Example 2 was performed, but 2 g of tocopheryl acetate was added.

[Example 20]

The same method as Example 2 was carried out, except that 2 g of sorbitan sesquioleate was added.

[Example 21]

The same method as Example 2 was performed, except that 2 g of copper tripeptide-1 was added.

[Example 22]

The same method as Example 2 was performed, except that 2 g of snake venom peptide was added.

[Example 23]

It was carried out in the same manner as in Example 2, but the illite was pulverized to a diameter of 0.2 mm, 200 g of the pulverized illite and 400 g of purified water were placed in a heating furnace, and then heated at 250° C. at 2 atm for 20 minutes to produce steam. After generation, the generated vapor was collected for 1 hour, cooled to room temperature, and condensed to further mix with 2.5 g of illite eluate.

[Example 24]

It was carried out in the same manner as in Example 2, except that 100 g of black beans were steamed at a temperature of 115 ° C. and a pressure of 3 kgf / cm ² for 4 minutes, cooled to a temperature of 30 ° C., and then the steamed black beans were incubated at 90 ° C. at a temperature of 250 ° C. After roasting for seconds, the roasted black beans are mixed with 7 times the weight of water, placed in a circulating extractor, cold extracted at a temperature of 25°C for 90 minutes, and then the black bean solids after the cold extraction are mixed with 26 times the weight of water. After hot water extraction at a temperature of 90°C for 15 minutes, 4 g of black bean extract prepared by mixing the cold-extracted extract and the hot-water extracted extract were further mixed.

[Example 25]

It was carried out in the same manner as in Example 2, but after washing the Baekbokryeong thoroughly with water, the Baekbokryeong was placed in a double boiler and heated in a double boiler at a temperature range of 80°C for 18 hours, and the leachate was placed in an extractor at a temperature range of 150°C and 4kgf/ 2 g of Baekbokryeong extract prepared by heating and extraction under a pressure of cm ² for 12 hours was further mixed.

[Example 26]

The same method as Example 2 was performed, except that 2.5 g of para-amino benzoic acid was added.

[Example 27]

This was carried out in the same manner as Example 2, except that 3 g of hyaluronic acid was added.

[Example 28]

The same method as Example 2 was performed, but 2 g of adenosine was additionally mixed.

[Example 29]

This was carried out in the same manner as in Example 2, except that 1 g of niacinamide was added.

[Example 30]

This was carried out in the same manner as in Example 2, but with an additional 1g of lactic acid added.

[Example 31]

This was carried out in the same manner as Example 2, except that all the adducts of Examples 3 to 30 were mixed.

[Comparative Example 1]

100 mL of distilled water was added to 5 g of ground stevia leaves, and then extracted in a water bath at about 80°C for about 4 hours.

Next, it was filtered through 5μm filter paper, and the filtered filtrate was concentrated under reduced pressure and then freeze-dried to prepare a stevia leaf hot water extract.

[Experiment 1]

The extraction yields of the stevia leaf subcritical water extract according to Example 1 and the stevia leaf hot water extract according to Comparative Example 1 were measured and shown in Table 1 below.

Example 1 Comparative Example 1 Extraction conditions 135℃, 7 MPa, 15 minutes 80℃, 4 hours transference number 43.0% 41.2%

As shown in Table 1, it was found that the yield of stevia leaf extract according to Example 1 was higher.

[Experiment 2]

To confirm the anti-inflammatory activity of the stevia subcritical water extract according to Example 1 and the extract according to Comparative Example 1, the NO production amount and cell survival rate of macrophages induced by LPS [lipopolysaccharide] were measured at a concentration of 100 to 400 μg/mL, respectively. .

Here, in the anti-inflammatory activity test, RAW264.7 cells, mouse macrophages, were purchased from ATCC [American Type Cell Culture]. The medium used to culture the cells was DMEM [Dulbecco's modified Eagle's medium] containing 100U/mL penicillin, 100μg/mL streptomycin, and 10% FBS [Fetal Bovine Serum], at 37°C. Cultured in a 5% carbon dioxide cell incubator. At this time, the cells were subcultured at 2-day intervals and used for experiments.

Next, RAW264.7 cells were distributed at 2.0 × 10 ⁵ cells/well in a 24-well plate and cultured for 24 hours at 37°C and 5% carbon dioxide.

Next, after culturing for 24 hours, the medium was replaced with a medium containing 100 ng/mL of LPS [lipopolysaccharide], and each sample was treated and cultured for 24 hours.

Next, the amount of NO (Nitric Oxide) produced was determined by mixing 100 μL of cell culture supernatant and 100 μL of Greiss reagent (1% sulfanilamide, 0.1% napthylethylene-diamine in 2.5% phosphoric acid) for 10 minutes in a 96-well plate. After reacting for a while, the absorbance was measured at 540 nm.

The amount of NO produced was measured in the form of NO ₂ ^- present in the cell culture medium, and quantified through a standard calibration curve prepared using sodium nitrite (NaNO ₂ ) as a standard material.

Meanwhile, cytotoxicity evaluation was conducted through MTT assay, in which cells were dispensed at 2.0 × 10 ⁵ cells/well and cultured in a cell incubator at 37°C and 5% carbon dioxide for 24 hours.

Next, after culturing for 24 hours, the medium was replaced with a medium containing 100 ng/mL of LPS [lipopolysaccharide], and each sample was treated and cultured for 24 hours.

Next, MTT [3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide] solution was added at a concentration of 500 μg/mL and cultured for an additional 4 hours.

After removing the medium containing the MTT solution, DMSO [dimethyl sulfoxide] was added to dissolve it, and the absorbance was measured at 570 nm.

The confirmed NO production rate, NO inhibition rate, and cytotoxicity evaluation results are shown in Table 2 and Figure 1.

NO production rate (%) NO inhibition rate (%) Cell survival rate (%) control group LPS (-) 2.1 97.9 110.1 LPS (+) 100.0 0.0 100.0 Comparative Example 1 100 μg/mL 81.5 18.5 103.9 200 μg/mL 64.3 35.7 106.2 300 μg/mL 59.0 41.0 106.0 400 μg/mL 55.6 44.4 105.5 Example 1 100 μg/mL 74.4 25.6 103.2 200 μg/mL 55.3 44.7 106.9 300 μg/mL 45.1 54.9 101.9 400 μg/mL 44.9 55.1 105.4

As shown in Table 2 and Figure 1, the hot water extract of Comparative Example 1 inhibited the production of NO by 44.4% at a concentration of 400 μg/mL, but the subcritical water extract of Example 1 inhibited the production of NO by 55.1% at a concentration of 400 μg/mL. It was confirmed that Through this, it was confirmed that the subcritical water extract had better anti-inflammatory efficacy at the same concentration, and that each extract had the effect of suppressing the production of NO in a concentration-dependent manner at a concentration of 100 to 400 μg/mL.

[Experiment 3]

The skin elasticity, skin glow, and cell membrane protection of the cosmetic compositions prepared according to the examples were measured, and the results are shown in Table 2.

Here, to measure skin elasticity, 20 women aged 20 to 55 with normal, oily, dry, and combination skin were asked to apply the product to the left and right eye corners twice a day. 14 days after each treatment, the skin elasticity of the skin surface was measured using Cutometer SEM 474. Skin elasticity measurement using Cutometer SEM 474 measures the depth of wrinkles. The smaller the value, the better the elasticity. The elasticity value is expressed as a percentage of the decrease compared to the control group. The average values of 20 subjects are shown in Table 1 below, and the control group represents the measurement values before processing the samples.

Here, the skin glow was expressed as the average value for 20 people by observing the appearance before and after applying the sample.

In addition, the cell membrane protection effect was measured according to the method of Park (Park, S. N, Journal of the Korean Cosmetic Society, 23(1), pp75-132, 1997).

The red blood cells (white blood cells removed) used in the experiment were collected from New Zealand albino rabbits. The red blood cell suspension had an O.D of 0.6 at 700 nm and the number of red blood cells was 1.5 × 107 cells/ml. 50㎕ of the extract was added to the red blood cell suspension, pre-incubated in the dark for 30 minutes, and then a photosensitizer (rose-bengal) was added and light irradiated. After light irradiation for 15 minutes, the degree of destruction of red blood cells by post-incubation was determined from the % transmittance at 700 nm at 15-minute intervals. The effect of the extract on photohemolysis was expressed as τ50, which represents the post-incubation time and degree of hemolysis, and the τ50 value was taken as the time (minutes) required for red blood cells to be 50% hemolyzed. 1,3-butylene glycol was used as a control.

Skin elasticity (wrinkle depth reduction) effect (%) skin glow Cell membrane protection
(τ50: min) 15th) 30(Sun) Example 1 9.4 12.2 good 284 Example 2 6.3 8.0 good 252 Example 3 6.6 8.1 good 242 Silencing example 4 6.7 8.3 good 255 Example 5 6.5 7.7 good 236 Example 6 6.6 7.8 good 244 Example 7 6.7 7.5 good 226 Example 8 6.5 7.7 good 247 Example 9 6.6 7.8 good 235 Example 10 6.8 7.6 good 242 Example 11 6.6 8.1 good 234 Example 12 6.7 8.4 good 235 Example 13 6.8 8.8 good 236 Example 14 6.7 7.8 good 237 Example 15 6.7 8.2 good 244 Example 16 6.8 8.0 good 249 Example 17 6.9 8.3 good 246 Example 18 6.7 8.1 good 234 Example 19 6.9 8.4 good 256 Example 20 6.6 8.5 good 246 Example 21 6.8 8.2 good 264 Example 22 6.6 7.9 good 256 Example 23 6.6 7.7 good 264 Example 24 6.9 8.1 good 236 Example 25 6.6 8.2 good 237 Example 26 6.6 7.8 good 241 Example 27 6.8 7.7 good 233 Example 28 6.7 8.2 good 246 Example 29 6.6 8.2 good 247 Example 30 6.7 8.0 good 234 Example 31 6.7 8.2 good 238 comparison 3.5 4.1 commonly 27

As shown in Table 3, the method for manufacturing a cosmetic composition containing the stevia leaf subcritical water extract prepared according to the examples was found to provide a cosmetic composition with good skin elasticity effect, skin glow, and cell membrane protection effect.

As described above, a person skilled in the art to which the present invention pertains will understand that the present invention can be implemented in other specific forms without changing its technical idea or essential features. Therefore, all embodiments described above should be understood as illustrative and not restrictive. The scope of the present invention should be construed as including all changes or modified forms derived from the meaning and scope of the claims described below and their equivalent concepts rather than the detailed description above.

Claims (5)

Based on 100 parts by weight of ground stevia leaves, adding 4,000 to 6,000 parts by weight of water, and extracting the stevia leaf subcritical water extract for 10 to 20 minutes at a pressure of 5 to 9 MPa and a temperature range of 130 to 140 ° C. A method for producing a cosmetic composition comprising a stevia leaf subcritical water extract.
According to paragraph 1,
A method for producing a cosmetic composition containing a subcritical water extract of stevia leaves, wherein the pulverized stevia leaves have an average particle diameter of 1 to 10 mm.
According to paragraph 2,
The pulverized stevia leaf is a method of producing a cosmetic composition containing a subcritical water extract of stevia leaf, which includes pulverizing stevia leaves dried by natural drying, hot air drying, or freeze drying.
Based on 100 parts by weight of purified water,
Based on 100 parts by weight of ground stevia leaves, 4,000 to 6,000 parts by weight of water was added, and 1 to 5 parts by weight of stevia leaf subcritical water extract extracted for 10 to 20 minutes at a pressure of 5 to 9 MPa and a temperature range of 130 to 140 ° C.; and
A method for producing a cosmetic composition containing a stevia leaf subcritical water extract, comprising the step of mixing 5 to 40 parts by weight of vegetable oil.
According to paragraph 4,
A method of producing a cosmetic composition comprising a stevia leaf subcritical water extract, wherein the vegetable oil is sunflower oil, coconut oil, castor oil, lavender oil, avocado oil, canola oil, grape seed oil, or a mixture thereof.