KR20240019808A - 생물학적 샘플을 분석하기 위한 시스템 및 방법 - Google Patents

생물학적 샘플을 분석하기 위한 시스템 및 방법 Download PDF

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Publication number
KR20240019808A
KR20240019808A KR1020247000613A KR20247000613A KR20240019808A KR 20240019808 A KR20240019808 A KR 20240019808A KR 1020247000613 A KR1020247000613 A KR 1020247000613A KR 20247000613 A KR20247000613 A KR 20247000613A KR 20240019808 A KR20240019808 A KR 20240019808A
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South Korea
Prior art keywords
nucleic acid
molecules
subset
cdna
derivatives
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KR1020247000613A
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English (en)
Korean (ko)
Inventor
피어 페데리코 게라르디니
타룬 쿠마르 쿠라나
알리 아가
일-쉬안 우
필리즈 고르페 야사르
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셀라노메, 인크.
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Publication of KR20240019808A publication Critical patent/KR20240019808A/ko
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
KR1020247000613A 2021-06-11 2022-06-10 생물학적 샘플을 분석하기 위한 시스템 및 방법 Pending KR20240019808A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163209544P 2021-06-11 2021-06-11
US63/209,544 2021-06-11
PCT/US2022/033116 WO2022261507A1 (en) 2021-06-11 2022-06-10 Systems and methods for analyzing biological samples

Publications (1)

Publication Number Publication Date
KR20240019808A true KR20240019808A (ko) 2024-02-14

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Family Applications (1)

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KR1020247000613A Pending KR20240019808A (ko) 2021-06-11 2022-06-10 생물학적 샘플을 분석하기 위한 시스템 및 방법

Country Status (11)

Country Link
US (1) US20240301465A1 (cg-RX-API-DMAC7.html)
EP (1) EP4352286A4 (cg-RX-API-DMAC7.html)
JP (1) JP2024520804A (cg-RX-API-DMAC7.html)
KR (1) KR20240019808A (cg-RX-API-DMAC7.html)
CN (1) CN117813424A (cg-RX-API-DMAC7.html)
AU (1) AU2022287983A1 (cg-RX-API-DMAC7.html)
BR (1) BR112023025731A2 (cg-RX-API-DMAC7.html)
CA (1) CA3220725A1 (cg-RX-API-DMAC7.html)
IL (1) IL308886A (cg-RX-API-DMAC7.html)
MX (1) MX2023014290A (cg-RX-API-DMAC7.html)
WO (1) WO2022261507A1 (cg-RX-API-DMAC7.html)

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US12576400B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for incubating and analyzing a cell in a compartment of a fluidic device
MX2023007842A (es) 2021-01-08 2023-09-19 Cellanome Inc Dispositivos y metodos para analizar muestras biologicas.
US12576399B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for imaging and analyzing a cell in a compartment of a fluidic device
US12661647B2 (en) 2022-03-22 2026-06-23 Cellanome, Inc. Methods for enclosing and analyzing a cell in a fluidic device
EP4558820A4 (en) * 2022-07-19 2026-07-15 Cellanome Inc SINGLE-CELL PROTEOMICS INVOLVING DEGRADABLE HYDROGELS
WO2024253995A1 (en) * 2023-06-09 2024-12-12 Illumina, Inc. Methods of sequencing dna using flow cells containing multiple surfaces
WO2025128916A1 (en) 2023-12-13 2025-06-19 Cellanome, Inc. Devices and methods for analyzing biological samples
WO2025231451A1 (en) * 2024-05-03 2025-11-06 Cellanome, Inc. Systems and methods for analysis of cells
WO2025229401A2 (en) * 2024-05-03 2025-11-06 Cellanome, Inc. Systems and methods for screening of biological components
WO2026103880A1 (zh) * 2024-11-15 2026-05-21 深圳华大智造科技股份有限公司 一种固体支持物及其用途

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US5766849A (en) * 1989-07-11 1998-06-16 Gen-Probe Incorporated Methods of amplifying nucleic acids using promoter-containing primer sequence
DK1907571T3 (en) * 2005-06-15 2017-08-21 Complete Genomics Inc NUCLEIC ACID ANALYSIS USING INCIDENTAL MIXTURES OF NON-OVERLAPPING FRAGMENTS
RU2008146089A (ru) * 2006-05-24 2010-05-27 Конинклейке Филипс Электроникс Н.В. (Nl) Твердая биосенсорная подложка с интегрированным регулированием температуры и способ ее изготовления
US20120028826A1 (en) * 2010-07-27 2012-02-02 Affymetrix, Inc. Methods and Compositions for Analysis of Nucleic Acids
GB201101621D0 (en) * 2011-01-31 2011-03-16 Olink Ab Method and product
CA2872915A1 (en) * 2012-05-09 2013-11-14 The Governors Of The University Of Alberta Solid gel amplification method and apparatus for platform molecular diagnostics
EP3901280B1 (en) * 2012-10-17 2025-03-12 10x Genomics Sweden AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
CA2901138A1 (en) * 2013-03-12 2014-10-09 Counsyl, Inc. Systems and methods for prenatal genetic analysis
WO2015065924A2 (en) * 2013-10-28 2015-05-07 Massachusetts Institute Of Technology Hydrogel microstructures with immiscible fluid isolation for small reaction volumes
US20170044525A1 (en) * 2014-04-29 2017-02-16 Illumina, Inc. Multiplexed single cell gene expression analysis using template switch and tagmentation
WO2015172080A1 (en) * 2014-05-08 2015-11-12 Fluidigm Corporation Integrated single cell sequencing
AU2015259024B2 (en) * 2014-05-16 2021-07-01 Illumina, Inc. Nucleic acid synthesis techniques
US10870848B2 (en) * 2015-09-15 2020-12-22 Takara Bio Usa, Inc. Methods for preparing a next generation sequencing (NGS) library from a ribonucleic acid (RNA) sample and compositions for practicing the same
CA3287905A1 (en) * 2016-10-14 2025-11-29 Wake Forest University Health Sciences Compositions, cell constructs, and methods of making and using the same
CN119842876A (zh) * 2017-08-01 2025-04-18 伊鲁米纳公司 使用的水凝胶珠和流动池的遗传物质的空间索引和文库制备
JP2021511823A (ja) * 2018-01-23 2021-05-13 バル・イラン・ユニバーシティＢａｒ Ｉｌａｎ Ｕｎｉｖｅｒｓｉｔｙ 細胞培養装置および方法
WO2019203728A1 (en) * 2018-04-20 2019-10-24 National University Of Singapore Linearization and labeling of single-stranded dna for optical sequence analysis
CN112585085A (zh) * 2018-08-06 2021-03-30 康宁股份有限公司 聚合水凝胶纳米结构阵列及其用途
CN113767175B (zh) * 2018-08-28 2025-07-25 10X基因组学股份有限公司 增加空间阵列分辨率
EP3938538A1 (en) * 2019-03-15 2022-01-19 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing
CN116685850A (zh) * 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析

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Publication number Publication date
BR112023025731A2 (pt) 2024-02-27
WO2022261507A1 (en) 2022-12-15
EP4352286A4 (en) 2025-04-23
EP4352286A1 (en) 2024-04-17
MX2023014290A (es) 2024-02-27
JP2024520804A (ja) 2024-05-24
CN117813424A (zh) 2024-04-02
AU2022287983A1 (en) 2023-12-14
IL308886A (en) 2024-01-01
CA3220725A1 (en) 2022-12-15
US20240301465A1 (en) 2024-09-12

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