CA3220725A1 - Systems and methods for analyzing biological samples - Google Patents
Systems and methods for analyzing biological samples Download PDFInfo
- Publication number
- CA3220725A1 CA3220725A1 CA3220725A CA3220725A CA3220725A1 CA 3220725 A1 CA3220725 A1 CA 3220725A1 CA 3220725 A CA3220725 A CA 3220725A CA 3220725 A CA3220725 A CA 3220725A CA 3220725 A1 CA3220725 A1 CA 3220725A1
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acid
- molecules
- subset
- cdna
- derivatives
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163209544P | 2021-06-11 | 2021-06-11 | |
| US63/209,544 | 2021-06-11 | ||
| PCT/US2022/033116 WO2022261507A1 (en) | 2021-06-11 | 2022-06-10 | Systems and methods for analyzing biological samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3220725A1 true CA3220725A1 (en) | 2022-12-15 |
Family
ID=84425364
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3220725A Pending CA3220725A1 (en) | 2021-06-11 | 2022-06-10 | Systems and methods for analyzing biological samples |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20240301465A1 (cg-RX-API-DMAC7.html) |
| EP (1) | EP4352286A4 (cg-RX-API-DMAC7.html) |
| JP (1) | JP2024520804A (cg-RX-API-DMAC7.html) |
| KR (1) | KR20240019808A (cg-RX-API-DMAC7.html) |
| CN (1) | CN117813424A (cg-RX-API-DMAC7.html) |
| AU (1) | AU2022287983A1 (cg-RX-API-DMAC7.html) |
| BR (1) | BR112023025731A2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA3220725A1 (cg-RX-API-DMAC7.html) |
| IL (1) | IL308886A (cg-RX-API-DMAC7.html) |
| MX (1) | MX2023014290A (cg-RX-API-DMAC7.html) |
| WO (1) | WO2022261507A1 (cg-RX-API-DMAC7.html) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024253995A1 (en) * | 2023-06-09 | 2024-12-12 | Illumina, Inc. | Methods of sequencing dna using flow cells containing multiple surfaces |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US12576400B2 (en) | 2021-01-08 | 2026-03-17 | Cellanome, Inc. | Methods for incubating and analyzing a cell in a compartment of a fluidic device |
| MX2023007842A (es) | 2021-01-08 | 2023-09-19 | Cellanome Inc | Dispositivos y metodos para analizar muestras biologicas. |
| US12576399B2 (en) | 2021-01-08 | 2026-03-17 | Cellanome, Inc. | Methods for imaging and analyzing a cell in a compartment of a fluidic device |
| US12661647B2 (en) | 2022-03-22 | 2026-06-23 | Cellanome, Inc. | Methods for enclosing and analyzing a cell in a fluidic device |
| EP4558820A4 (en) * | 2022-07-19 | 2026-07-15 | Cellanome Inc | SINGLE-CELL PROTEOMICS INVOLVING DEGRADABLE HYDROGELS |
| WO2025128916A1 (en) | 2023-12-13 | 2025-06-19 | Cellanome, Inc. | Devices and methods for analyzing biological samples |
| WO2025231451A1 (en) * | 2024-05-03 | 2025-11-06 | Cellanome, Inc. | Systems and methods for analysis of cells |
| WO2025229401A2 (en) * | 2024-05-03 | 2025-11-06 | Cellanome, Inc. | Systems and methods for screening of biological components |
| WO2026103880A1 (zh) * | 2024-11-15 | 2026-05-21 | 深圳华大智造科技股份有限公司 | 一种固体支持物及其用途 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5766849A (en) * | 1989-07-11 | 1998-06-16 | Gen-Probe Incorporated | Methods of amplifying nucleic acids using promoter-containing primer sequence |
| DK1907571T3 (en) * | 2005-06-15 | 2017-08-21 | Complete Genomics Inc | NUCLEIC ACID ANALYSIS USING INCIDENTAL MIXTURES OF NON-OVERLAPPING FRAGMENTS |
| RU2008146089A (ru) * | 2006-05-24 | 2010-05-27 | Конинклейке Филипс Электроникс Н.В. (Nl) | Твердая биосенсорная подложка с интегрированным регулированием температуры и способ ее изготовления |
| US20120028826A1 (en) * | 2010-07-27 | 2012-02-02 | Affymetrix, Inc. | Methods and Compositions for Analysis of Nucleic Acids |
| GB201101621D0 (en) * | 2011-01-31 | 2011-03-16 | Olink Ab | Method and product |
| CA2872915A1 (en) * | 2012-05-09 | 2013-11-14 | The Governors Of The University Of Alberta | Solid gel amplification method and apparatus for platform molecular diagnostics |
| EP3901280B1 (en) * | 2012-10-17 | 2025-03-12 | 10x Genomics Sweden AB | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| CA2901138A1 (en) * | 2013-03-12 | 2014-10-09 | Counsyl, Inc. | Systems and methods for prenatal genetic analysis |
| WO2015065924A2 (en) * | 2013-10-28 | 2015-05-07 | Massachusetts Institute Of Technology | Hydrogel microstructures with immiscible fluid isolation for small reaction volumes |
| US20170044525A1 (en) * | 2014-04-29 | 2017-02-16 | Illumina, Inc. | Multiplexed single cell gene expression analysis using template switch and tagmentation |
| WO2015172080A1 (en) * | 2014-05-08 | 2015-11-12 | Fluidigm Corporation | Integrated single cell sequencing |
| AU2015259024B2 (en) * | 2014-05-16 | 2021-07-01 | Illumina, Inc. | Nucleic acid synthesis techniques |
| US10870848B2 (en) * | 2015-09-15 | 2020-12-22 | Takara Bio Usa, Inc. | Methods for preparing a next generation sequencing (NGS) library from a ribonucleic acid (RNA) sample and compositions for practicing the same |
| CA3287905A1 (en) * | 2016-10-14 | 2025-11-29 | Wake Forest University Health Sciences | Compositions, cell constructs, and methods of making and using the same |
| CN119842876A (zh) * | 2017-08-01 | 2025-04-18 | 伊鲁米纳公司 | 使用的水凝胶珠和流动池的遗传物质的空间索引和文库制备 |
| JP2021511823A (ja) * | 2018-01-23 | 2021-05-13 | バル・イラン・ユニバーシティＢａｒ Ｉｌａｎ Ｕｎｉｖｅｒｓｉｔｙ | 細胞培養装置および方法 |
| WO2019203728A1 (en) * | 2018-04-20 | 2019-10-24 | National University Of Singapore | Linearization and labeling of single-stranded dna for optical sequence analysis |
| CN112585085A (zh) * | 2018-08-06 | 2021-03-30 | 康宁股份有限公司 | 聚合水凝胶纳米结构阵列及其用途 |
| CN113767175B (zh) * | 2018-08-28 | 2025-07-25 | 10X基因组学股份有限公司 | 增加空间阵列分辨率 |
| EP3938538A1 (en) * | 2019-03-15 | 2022-01-19 | 10X Genomics, Inc. | Methods for using spatial arrays for single cell sequencing |
| CN116685850A (zh) * | 2020-12-15 | 2023-09-01 | 贝克顿迪金森公司 | 单细胞分泌组分析 |
-
2022
- 2022-06-10 EP EP22821163.7A patent/EP4352286A4/en active Pending
- 2022-06-10 MX MX2023014290A patent/MX2023014290A/es unknown
- 2022-06-10 KR KR1020247000613A patent/KR20240019808A/ko active Pending
- 2022-06-10 BR BR112023025731A patent/BR112023025731A2/pt unknown
- 2022-06-10 WO PCT/US2022/033116 patent/WO2022261507A1/en not_active Ceased
- 2022-06-10 CA CA3220725A patent/CA3220725A1/en active Pending
- 2022-06-10 CN CN202280055747.XA patent/CN117813424A/zh active Pending
- 2022-06-10 AU AU2022287983A patent/AU2022287983A1/en active Pending
- 2022-06-10 JP JP2023575822A patent/JP2024520804A/ja active Pending
- 2022-06-10 IL IL308886A patent/IL308886A/en unknown
-
2023
- 2023-12-08 US US18/534,328 patent/US20240301465A1/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024253995A1 (en) * | 2023-06-09 | 2024-12-12 | Illumina, Inc. | Methods of sequencing dna using flow cells containing multiple surfaces |
Also Published As
| Publication number | Publication date |
|---|---|
| BR112023025731A2 (pt) | 2024-02-27 |
| WO2022261507A1 (en) | 2022-12-15 |
| EP4352286A4 (en) | 2025-04-23 |
| KR20240019808A (ko) | 2024-02-14 |
| EP4352286A1 (en) | 2024-04-17 |
| MX2023014290A (es) | 2024-02-27 |
| JP2024520804A (ja) | 2024-05-24 |
| CN117813424A (zh) | 2024-04-02 |
| AU2022287983A1 (en) | 2023-12-14 |
| IL308886A (en) | 2024-01-01 |
| US20240301465A1 (en) | 2024-09-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20240301465A1 (en) | Systems and methods for analyzing biological samples | |
| US11873530B1 (en) | Systems and methods for metabolome analysis | |
| US12606860B2 (en) | Systems and methods for processing RNA from cells | |
| US11155810B2 (en) | Single cell analysis of transposase accessible chromatin | |
| US11773389B2 (en) | Single cell analysis of transposase accessible chromatin | |
| KR102852123B1 (ko) | Dna 가공을 위한 중합체 비드의 조절 | |
| CN105492607B (zh) | 用于样品处理的组合物和方法 | |
| CN111108219B (zh) | 使用的水凝胶珠和流动池的遗传物质的空间索引和文库制备 | |
| RU2750567C2 (ru) | Способы инкапсулирования одиночных клеток, инкапсулированные клетки и способы их применения | |
| CN107427808B (zh) | 用于制备核酸测序文库的方法和系统以及用其制备的文库 | |
| JP7773373B2 (ja) | オンフローセル三次元ポリマー構造 | |
| CN113874521A (zh) | 用于富集条形码的方法和系统 | |
| CN114616341A (zh) | 联接介导的核酸分析 | |
| CN113767178A (zh) | 用于处理光学标签化珠粒的系统和方法 | |
| WO2018218226A1 (en) | Single cell analysis of transposase accessible chromatin | |
| US20250050301A1 (en) | Spatial barcodes by hydrogel lithography | |
| RU2822154C2 (ru) | Модуляция полимерных гранул для обработки днк |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 3RD ANNIV.) - STANDARD Year of fee payment: 3 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20250606 |
|
| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20250606 |
|
| D11 | Substantive examination requested |
Free format text: ST27 STATUS EVENT CODE: A-1-1-D10-D11-D117 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: REQUEST FOR EXAMINATION RECEIVED Effective date: 20260529 |
|
| P11 | Amendment of application requested |
Free format text: ST27 STATUS EVENT CODE: A-1-1-P10-P11-P101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT RECEIVED - VOLUNTARY AMENDMENT Effective date: 20260529 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-1-1-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT Effective date: 20260529 |
|
| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 4TH ANNIV.) - STANDARD Year of fee payment: 4 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20260605 |
|
| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20260605 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-1-1-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT Effective date: 20260622 |
|
| D00 | Search and/or examination requested or commenced |
Free format text: ST27 STATUS EVENT CODE: A-1-1-D10-D00-D118 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: REQUEST FOR EXAMINATION REQUIREMENTS DETERMINED COMPLIANT Effective date: 20260713 |
|
| D11 | Substantive examination requested |
Free format text: ST27 STATUS EVENT CODE: A-1-2-D10-D11-D155 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: ALL REQUIREMENTS FOR EXAMINATION DETERMINED COMPLIANT Effective date: 20260713 |
|
| P11 | Amendment of application requested |
Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT DETERMINED COMPLIANT Effective date: 20260713 |
|
| P13 | Application amended |
Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P13-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: APPLICATION AMENDED Effective date: 20260713 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT Effective date: 20260713 |