JP2024520804A - 生体試料を分析するためのシステムおよび方法 - Google Patents

生体試料を分析するためのシステムおよび方法 Download PDF

Info

Publication number
JP2024520804A
JP2024520804A JP2023575822A JP2023575822A JP2024520804A JP 2024520804 A JP2024520804 A JP 2024520804A JP 2023575822 A JP2023575822 A JP 2023575822A JP 2023575822 A JP2023575822 A JP 2023575822A JP 2024520804 A JP2024520804 A JP 2024520804A
Authority
JP
Japan
Prior art keywords
nucleic acid
molecules
subset
cdna
derivatives
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2023575822A
Other languages
English (en)
Japanese (ja)
Other versions
JP2024520804A5 (cg-RX-API-DMAC7.html
Inventor
ピエル フェデリコ ゲラルディーニ，
タルン クマール クラナ，
アリ アガー，
イル－シュアン ウー，
フィリズ ゴルぺ ヤサル，
Original Assignee
セラノーム， インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by セラノーム， インコーポレイテッド filed Critical セラノーム， インコーポレイテッド
Publication of JP2024520804A publication Critical patent/JP2024520804A/ja
Publication of JP2024520804A5 publication Critical patent/JP2024520804A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2023575822A 2021-06-11 2022-06-10 生体試料を分析するためのシステムおよび方法 Pending JP2024520804A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163209544P 2021-06-11 2021-06-11
US63/209,544 2021-06-11
PCT/US2022/033116 WO2022261507A1 (en) 2021-06-11 2022-06-10 Systems and methods for analyzing biological samples

Publications (2)

Publication Number Publication Date
JP2024520804A true JP2024520804A (ja) 2024-05-24
JP2024520804A5 JP2024520804A5 (cg-RX-API-DMAC7.html) 2025-06-18

Family

ID=84425364

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023575822A Pending JP2024520804A (ja) 2021-06-11 2022-06-10 生体試料を分析するためのシステムおよび方法

Country Status (11)

Country Link
US (1) US20240301465A1 (cg-RX-API-DMAC7.html)
EP (1) EP4352286A4 (cg-RX-API-DMAC7.html)
JP (1) JP2024520804A (cg-RX-API-DMAC7.html)
KR (1) KR20240019808A (cg-RX-API-DMAC7.html)
CN (1) CN117813424A (cg-RX-API-DMAC7.html)
AU (1) AU2022287983A1 (cg-RX-API-DMAC7.html)
BR (1) BR112023025731A2 (cg-RX-API-DMAC7.html)
CA (1) CA3220725A1 (cg-RX-API-DMAC7.html)
IL (1) IL308886A (cg-RX-API-DMAC7.html)
MX (1) MX2023014290A (cg-RX-API-DMAC7.html)
WO (1) WO2022261507A1 (cg-RX-API-DMAC7.html)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12576400B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for incubating and analyzing a cell in a compartment of a fluidic device
MX2023007842A (es) 2021-01-08 2023-09-19 Cellanome Inc Dispositivos y metodos para analizar muestras biologicas.
US12576399B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for imaging and analyzing a cell in a compartment of a fluidic device
US12661647B2 (en) 2022-03-22 2026-06-23 Cellanome, Inc. Methods for enclosing and analyzing a cell in a fluidic device
EP4558820A4 (en) * 2022-07-19 2026-07-15 Cellanome Inc SINGLE-CELL PROTEOMICS INVOLVING DEGRADABLE HYDROGELS
WO2024253995A1 (en) * 2023-06-09 2024-12-12 Illumina, Inc. Methods of sequencing dna using flow cells containing multiple surfaces
WO2025128916A1 (en) 2023-12-13 2025-06-19 Cellanome, Inc. Devices and methods for analyzing biological samples
WO2025231451A1 (en) * 2024-05-03 2025-11-06 Cellanome, Inc. Systems and methods for analysis of cells
WO2025229401A2 (en) * 2024-05-03 2025-11-06 Cellanome, Inc. Systems and methods for screening of biological components
WO2026103880A1 (zh) * 2024-11-15 2026-05-21 深圳华大智造科技股份有限公司 一种固体支持物及其用途

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5908744A (en) * 1989-07-11 1999-06-01 Gen-Probe Incorporated Detecting Mycobacterium tuberculosis by nucleic acid sequence amplification
JP2009538126A (ja) * 2006-05-24 2009-11-05 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 統合された温度制御を備えたバイオセンサーの固体の基体及びそれを作るための方法
US20130296172A1 (en) * 2010-07-27 2013-11-07 Affymetrix, Inc. Methods and Compositions for Analysis of Nucleic Acids
US20150240289A1 (en) * 2012-05-09 2015-08-27 The Governors Of The University Of Alberta Solid gel amplification method and apparatus for platform molecular diagnostics
JP2016513461A (ja) * 2013-03-12 2016-05-16 カウンシル，インコーポレーテッド 出生前遺伝子分析システム及び方法
JP2017514478A (ja) * 2014-05-08 2017-06-08 フリューダイム・コーポレイション 集積した単一細胞の配列決定
US20190345439A1 (en) * 2016-10-14 2019-11-14 Wake Forest University Health Sciences Compositions, cell constructs, and methods of making and using the same
US20200399571A1 (en) * 2018-01-23 2020-12-24 Bar-Ilan University Cell culturing device and method
CN112585085A (zh) * 2018-08-06 2021-03-30 康宁股份有限公司 聚合水凝胶纳米结构阵列及其用途

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1907571T3 (en) * 2005-06-15 2017-08-21 Complete Genomics Inc NUCLEIC ACID ANALYSIS USING INCIDENTAL MIXTURES OF NON-OVERLAPPING FRAGMENTS
GB201101621D0 (en) * 2011-01-31 2011-03-16 Olink Ab Method and product
EP3901280B1 (en) * 2012-10-17 2025-03-12 10x Genomics Sweden AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
WO2015065924A2 (en) * 2013-10-28 2015-05-07 Massachusetts Institute Of Technology Hydrogel microstructures with immiscible fluid isolation for small reaction volumes
US20170044525A1 (en) * 2014-04-29 2017-02-16 Illumina, Inc. Multiplexed single cell gene expression analysis using template switch and tagmentation
AU2015259024B2 (en) * 2014-05-16 2021-07-01 Illumina, Inc. Nucleic acid synthesis techniques
US10870848B2 (en) * 2015-09-15 2020-12-22 Takara Bio Usa, Inc. Methods for preparing a next generation sequencing (NGS) library from a ribonucleic acid (RNA) sample and compositions for practicing the same
CN119842876A (zh) * 2017-08-01 2025-04-18 伊鲁米纳公司 使用的水凝胶珠和流动池的遗传物质的空间索引和文库制备
WO2019203728A1 (en) * 2018-04-20 2019-10-24 National University Of Singapore Linearization and labeling of single-stranded dna for optical sequence analysis
CN113767175B (zh) * 2018-08-28 2025-07-25 10X基因组学股份有限公司 增加空间阵列分辨率
EP3938538A1 (en) * 2019-03-15 2022-01-19 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing
CN116685850A (zh) * 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5908744A (en) * 1989-07-11 1999-06-01 Gen-Probe Incorporated Detecting Mycobacterium tuberculosis by nucleic acid sequence amplification
JP2009538126A (ja) * 2006-05-24 2009-11-05 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 統合された温度制御を備えたバイオセンサーの固体の基体及びそれを作るための方法
US20130296172A1 (en) * 2010-07-27 2013-11-07 Affymetrix, Inc. Methods and Compositions for Analysis of Nucleic Acids
US20150240289A1 (en) * 2012-05-09 2015-08-27 The Governors Of The University Of Alberta Solid gel amplification method and apparatus for platform molecular diagnostics
JP2016513461A (ja) * 2013-03-12 2016-05-16 カウンシル，インコーポレーテッド 出生前遺伝子分析システム及び方法
JP2017514478A (ja) * 2014-05-08 2017-06-08 フリューダイム・コーポレイション 集積した単一細胞の配列決定
US20190345439A1 (en) * 2016-10-14 2019-11-14 Wake Forest University Health Sciences Compositions, cell constructs, and methods of making and using the same
US20200399571A1 (en) * 2018-01-23 2020-12-24 Bar-Ilan University Cell culturing device and method
CN112585085A (zh) * 2018-08-06 2021-03-30 康宁股份有限公司 聚合水凝胶纳米结构阵列及其用途

Also Published As

Publication number Publication date
BR112023025731A2 (pt) 2024-02-27
WO2022261507A1 (en) 2022-12-15
EP4352286A4 (en) 2025-04-23
KR20240019808A (ko) 2024-02-14
EP4352286A1 (en) 2024-04-17
MX2023014290A (es) 2024-02-27
CN117813424A (zh) 2024-04-02
AU2022287983A1 (en) 2023-12-14
IL308886A (en) 2024-01-01
CA3220725A1 (en) 2022-12-15
US20240301465A1 (en) 2024-09-12

Similar Documents

Publication Publication Date Title
US20240301465A1 (en) Systems and methods for analyzing biological samples
US11873530B1 (en) Systems and methods for metabolome analysis
US12606860B2 (en) Systems and methods for processing RNA from cells
KR102852123B1 (ko) Dna 가공을 위한 중합체 비드의 조절
CN111108219B (zh) 使用的水凝胶珠和流动池的遗传物质的空间索引和文库制备
CN105492607B (zh) 用于样品处理的组合物和方法
CN107427808B (zh) 用于制备核酸测序文库的方法和系统以及用其制备的文库
US20200248176A1 (en) Single cell analysis of transposase accessible chromatin
CN115916972A (zh) 用于被固定样品的组合物和方法
CN114616341A (zh) 联接介导的核酸分析
JP2022105000A (ja) 単一細胞を封入する方法、封入された細胞およびその使用
JP7773373B2 (ja) オンフローセル三次元ポリマー構造
CN113874521A (zh) 用于富集条形码的方法和系统
US20250050301A1 (en) Spatial barcodes by hydrogel lithography
WO2023183327A1 (en) Multiplex cellular assays for screening and quality assessment of engineered cells
WO2024145393A1 (en) Spatially barcoded surfaces
RU2822154C2 (ru) Модуляция полимерных гранул для обработки днк
CN117015615A (zh) 用于分析生物样品的装置和方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250610

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20250610

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20260430

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20260501

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20260731