JP2021511823A - 細胞培養装置および方法 - Google Patents
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Abstract
Description
貫通孔を備えた、細胞培養プレートの作製
マイクロチャンバの作製
PDMSスタンプヘッドは、異なる直径でプレキシガラスから作製されたシリンダに接着された。シリンダの他方の端部は、プレキシガラスホルダ(Plexiglas holder)に接着された。6ウェルプレートのためには、シリンダは直径10mmで、24ウェルプレートのためには、シリンダは直径6mmだった(図7のAのそれぞれの平面図および底面図)。それに応じて、PDMSスタンプヘッドのサイズが決められた。
65−70℃に予熱された低融点アガロース溶液(6%)の小滴(24ウェルプレート用では±70ul、6ウェルプレート用では±400ul)が、ドライバス(dry bath)で80℃に予熱されたガラス底部の表面の貫通孔の中に、対称的に落とされた(図8)。
浸潤分析のための培養プレートの作製
共培養分析のための、培養プレートの作製
細胞を備えたプレート
浸潤分析プロトコル
PrCSCスフェロイド、前立腺癌関連線維芽細胞(PCAF)、および炎症細胞の共培養
薬物スクリーニング
PrTMICの回収、富化および分子分析
乳癌の多細胞3D構造、形成、およびインビトロ成長に対する、環境剛性の影響
Claims (29)
- 細胞培養装置であって:
(a)底壁に形成された貫通孔を具備する少なくとも1つのウェルを有するプレートと;
(b)前記貫通孔に配置されたヒドロゲルマトリックスと
を含む、細胞培養装置。 - (c)前記ヒドロゲルマトリックスに形成された少なくとも1つのチャンバ
をさらに含む、請求項1に記載の細胞培養装置。 - 前記貫通孔が、その中に前記ヒドロゲルマトリックスを閉じ込めるように形作られている、請求項1に記載の細胞培養装置。
- 前記ヒドロゲルマトリックスが、前記少なくとも1つのウェルの中に広がる、請求項1に記載の細胞培養装置。
- 前記プレートの下に位置する光学的に透明な支持部をさらに含み、ここで、前記貫通孔に配置された前記ヒドロゲルマトリックスが前記支持部の上面に接触する、請求項1に記載の細胞培養装置。
- 前記貫通孔が、円すい台として形作られている、請求項1に記載の細胞培養装置。
- 前記貫通孔が、2−32mmに及ぶ直径を有する、請求項6に記載の細胞培養装置。
- 前記貫通孔の内面が、少なくとも1つのアンダーカット領域を含む、請求項3に記載の細胞培養装置。
- 前記アンダーカットが、0.5−2mmの深さを有する、請求項8に記載の細胞培養装置。
- 前記貫通孔の内面が、半径方向内側に向けられた突起を含む、請求項3に記載の細胞培養装置。
- 前記突起が、長さ0.5−3.5mmである、請求項10に記載の細胞培養装置。
- 前記ヒドロゲルマトリックスに形成された、複数のピコリットルからマイクロリットルのチャンバを含む、請求項2に記載の細胞培養装置。
- 前記ピコリットルからマイクロリットルのチャンバが、逆さまの角すい台として形作られる、請求項1に記載の細胞培養装置。
- 前記ピコリットルからマイクロリットルのチャンバの各々が、1−50ナノリットルに及ぶ容積を有する、請求項13に記載の細胞培養装置。
- 前記少なくとも1つのウェルに位置し得るリングをさらに含み、前記リングが円周方向の内側溝を含む、請求項1に記載の細胞培養装置。
- 前記リングが、直径2−32mmである、請求項15に記載の細胞培養装置。
- 前記少なくとも1つのウェルに位置し得るダブルリングインサートをさらに含み、前記ダブルリングインサートが、前記ダブルリングインサートの内側リングによって画定される中央開口、および、前記ダブルリングインサートの前記内側リングと外側リングとの間に画定される複数の区画を含む、請求項1に記載の細胞培養装置。
- 前記ダブルリングインサートが、前記内側リングの内壁内に、少なくとも1つの円周方向の溝を含む、請求項17に記載の細胞培養装置。
- 1つ以上の細胞型を培養する方法であって:
(a)請求項12に記載の細胞培養装置を供する工程と;
(b)前記ピコリットルからマイクロリットルのチャンバ内に、1つ以上の細胞型を播種する工程と;
(c)細胞培養装置を,前記1つ以上の細胞型を培養するのに適した条件にさらす工程と
を含む、方法。 - 前記チャンバが、各々が1つの細胞型を播種するためのものである複数の区画内に形成される、請求項19に記載の方法。
- 培養装置を製造する方法であって:
(a)底壁に形成された貫通孔を具備する少なくとも1つのウェルを有するプレートを供する工程と;
(b)前記貫通孔をヒドロゲルで満たす工程と;
(c)前記ヒドロゲルの中の少なくとも1つの細胞培養チャンバにエンボス加工する工程と
を含む、方法。 - (b)の前に、前記ウェル内にダブルリングインサートを配置する工程をさらに含む、請求項21に記載の方法。
- 前記ダブルリングインサートが、前記ダブルリングインサートの内側リングによって画定される中央開口、および、前記ダブルリングインサートの前記内側リングと外側リングとの間に画定される複数の区画を含む、請求項22に記載の方法。
- 前記ダブルリングインサートが、前記ヒドロゲルを閉じ込めるために、前記内側リングの内壁内に、少なくとも1つの円周方向の溝を含む、請求項23に記載の方法。
- 細胞培養装置であって:
(a)底壁に形成された貫通孔を具備する少なくとも1つのウェルを有するプレートと;
(b)前記貫通孔に配置されたヒドロゲルマトリックスと;
(c)前記ヒドロゲルマトリックスの上部に配置されたゲルと
を含む、細胞培養装置。 - 前記ゲルが、少なくとも1つの細胞外マトリックス(ECM)成分を含む、請求項25に記載の細胞培養装置。
- 少なくとも1つの細胞外マトリックス(ECM)成分を含む前記ゲルが、前記ゲルを閉じ込めるために、リングの内面に沿って連続している/セグメント化されている、円周方向の溝を含むリング内に配置されている、請求項26に記載の細胞培養装置。
- 少なくとも1つの細胞外マトリックス(ECM)成分を含む前記ゲルが、前記少なくとも1つのウェルに位置するダブルリングインサート内に配置され、前記ダブルリングインサートが、前記ダブルリングインサートの内側リングによって画定される中央開口、および、前記ダブルリングインサートの前記内側リングと外側リングとの間に画定される複数の区画を含む、請求項25に記載の細胞培養装置。
- 前記ダブルリングインサートが、少なくとも1つの細胞外マトリックス(ECM)成分を含む前記ゲルを閉じ込めるために、前記内側リングの内壁内に少なくとも1つの円周方向の溝を含む、請求項28に記載の細胞培養装置。
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Publication number | Priority date | Publication date | Assignee | Title |
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JP2007515958A (ja) * | 2003-12-19 | 2007-06-21 | ユニヴァーシティー オブ ウォータールー | 培養細胞、細胞培養の方法および機器 |
US20100055790A1 (en) * | 2006-11-14 | 2010-03-04 | Simon Eric M | Cell culture apparatus and associated methods |
JP2010537633A (ja) * | 2007-08-30 | 2010-12-09 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | コンプライアント表面のマルチウェル培養プレート |
US20150125952A1 (en) * | 2012-04-04 | 2015-05-07 | University Of Washington Through Its Center For Commercialization | Systems and method for engineering muscle tissue |
WO2016103002A1 (en) * | 2014-12-22 | 2016-06-30 | Ecole Polytechnique Federale De Lausanne (Epfl) | Devices for high-throughput aggregation and manipulation of mammalian cells |
WO2016145077A1 (en) * | 2015-03-09 | 2016-09-15 | University Of Washington | Micro-and nanopatterned substrates for cell migration and uses thereof |
WO2017005927A1 (en) * | 2015-07-08 | 2017-01-12 | Paris Sciences Et Lettres - Quartier Latin | Cell culture device |
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US20100055790A1 (en) * | 2006-11-14 | 2010-03-04 | Simon Eric M | Cell culture apparatus and associated methods |
JP2010537633A (ja) * | 2007-08-30 | 2010-12-09 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | コンプライアント表面のマルチウェル培養プレート |
US20150125952A1 (en) * | 2012-04-04 | 2015-05-07 | University Of Washington Through Its Center For Commercialization | Systems and method for engineering muscle tissue |
WO2016103002A1 (en) * | 2014-12-22 | 2016-06-30 | Ecole Polytechnique Federale De Lausanne (Epfl) | Devices for high-throughput aggregation and manipulation of mammalian cells |
WO2016145077A1 (en) * | 2015-03-09 | 2016-09-15 | University Of Washington | Micro-and nanopatterned substrates for cell migration and uses thereof |
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