KR20240017189A - Functional feed additive using the soldier fly and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a functional feed additive using black soldier fly larvae, which has a high GABA (Gamma Aminobutyric Acid) content, so that it can be used as a functional additive for animal feed, and a method for manufacturing the same.
For this purpose, the present invention is Limosilactobacillus reuteri subsp. reuteri and Lactiplantibacillus plantarum subsp. Process of culturing Plantarum strains at 37°C for 2 days; A process of mixing the cultured strain with dry food waste powder; A process of preparing food by mixing MGS (Mono Sodium Glutamate) with the mixture of the strain and dried food waste powder and adding soybean oil meal, rice bran oil meal, and rapeseed oil meal, respectively; The process of feeding black soldier fly larvae 4 days after hatching with the above food and rearing them for 10 to 14 days; It consists of the process of selecting larvae from reared black soldier flies and then drying them with hot air.

Description

Functional feed additive using the soldier fly and manufacturing method thereof}

The present invention relates to a functional feed additive using black soldier fly larvae, which has a high GABA (Gamma Aminobutyric Acid) content, so that it can be used as a functional additive for animal feed, and a method for manufacturing the same.

Black soldier flies are a general term for insects belonging to the family Diptera, black soldier flies, and live in food waste and organic waste. Adults do not transmit diseases and, unlike flies, are treated as sanitary insects as they do not cause harm to humans.

These black soldier fly larvae have a strong ability to decompose organic matter, so even the feed needed for breeding can be used as food waste and other organic matter, so it is used as a high-income material with very high eco-friendly and economic benefits.

In addition, the life cycle of black soldier flies is egg (4-5 days), larva (14 days), pupa (14 days), and adult (5-8 days). They survive for approximately 37 to 41 days, and commercialization is mainly done. The period to the larval stage is very short, about 10 to 14 days, and each animal lays 1,000 eggs, making it a highly productive material.

In addition, black soldier flies contain a lot of lauric acid as a protein and fatty acid component, and this lauric acid is known to be effective as a natural antibacterial agent, so it has recently been used as feed for livestock, poultry, fish, and pets. It is becoming.

As an example, in Republic of Korea Patent Publication No. 10-2022-0091321 (Method for manufacturing feed using black soldier fly, etc.), black soldier fly larvae are washed, dried naturally to remove moisture, and then sterilized in high-temperature, high-pressure steam or low-temperature, low-pressure area, A method of manufacturing feed was proposed by granulating or powdering larvae in sterilized black soldier flies and packaging them.

However, this prior art is a method of simply processing black soldier flies into animal feed, and when used as animal feed, it does not have the functionality to improve stress and sleep disorders caused by dense feeding of animals, resulting in a mortality rate. There are increasing problems.

<Prior art literature>

1. Republic of Korea Patent Publication No. 10-2022-0091321

(Feed manufacturing method using black soldier flies)

In order to solve this conventional problem, the present invention mixes food waste fed to black soldier fly larvae with a strain culture medium, dries it, mixes it with MSG (Mono Sodium Glutamate), and feeds black soldier fly larvae with this mixture, which contains GABA. The purpose is to provide a functional feed additive using black soldier fly and a manufacturing method thereof that can improve stress and sleep disorders in animals by using the powder of black soldier fly larvae containing GABA as a feed additive.

The functional feed additive using Dongae, etc. according to the present invention to achieve the above purpose,

Four days after hatching, black soldier fly larvae are fed MGS (Mono Sodium Glutamate) and additionally one or more of soybean oil meal, rice bran oil meal, and rapeseed oil meal to ensure high GABA content, and the larvae are dried on black soldier fly butterfly containing GABA. It is characterized by being manufactured.

In addition, the method for manufacturing functional feed additives using Dongae,

Limosilactobacillus reuteri subsp. reuteri and Lactiplantibacillus plantarum subsp. Process of culturing Plantarum strains at 37°C for 2 days;

A process of mixing the cultured strain with dry food waste powder;

A process of preparing food by mixing MGS (Mono Sodium Glutamate) with the mixture of the strain and dried food waste powder and adding soybean oil meal, rice bran oil meal, and rapeseed oil meal, respectively;

The process of feeding black soldier fly larvae 4 days after hatching with the above food and rearing them for 10 to 14 days;

It is characterized by consisting of a process of selecting larvae from reared black soldier flies and then drying them with hot air.

In addition, the food waste is dried and powdered after removing salt, and molasses is further added to the mixture of the strain and dried food waste powder to reach 30 to 50 ppm.

According to the present invention constructed as described above, a functional feed additive containing a high content of GABA, an inhibitory neurotransmitter of the paravertebral nervous system, is manufactured by feeding black soldier flies with MSG and protein, and this is added to the feed to provide emotional stability and anti-inflammatory properties for animals. Induces mental anxiety and promotes sleep.

As a result, it is possible to improve stress and sleep disorders in livestock, as well as farmed fish and companion animals, and also has the advantage of preventing death due to stress.

Figure 1 is a diagram illustrating the process of producing a feed additive containing GABA through bioconversion by intestinal lactic acid bacteria by feeding MSG to black soldier flies according to the present invention.
Figure 2 shows the results of GABA qualitative analysis of extracts extracted from feed additives according to the present invention.
Figure 3 shows the results of GABA quantitative analysis from feed additive extract.
Figure 4 is a barplot showing the classification of intestinal bacteria of black soldier fly larvae without MSG and with MSG.

The above-mentioned objects, features, and advantages will be described in detail later with reference to the attached drawings, so that those skilled in the art will be able to easily implement the technical idea of the present invention.

In describing the present invention, if it is determined that a detailed description of known technologies related to the present invention may unnecessarily obscure the gist of the present invention, the detailed description will be omitted.

The terms used in the present invention are general terms that are currently widely used as much as possible while considering the function in the present invention, but this may vary depending on the intention or precedent of a person working in the art, the emergence of new technology, etc.

In addition, in certain cases, there are terms arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the relevant invention.

Therefore, the terms used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than simply the name of the term.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

However, the embodiments of the present invention illustrated below may be modified into various other forms, and the scope of the present invention is not limited to the embodiments detailed below.

Examples of the present invention are provided to more completely explain the present invention to those skilled in the art.

Figure 1 is a diagram illustrating the process of feeding MSG to black soldier flies according to the present invention and producing a feed additive containing GABA through bioconversion by intestinal lactic acid bacteria, and Figure 2 is an extract extracted from the feed additive according to the present invention. A diagram showing the results of GABA qualitative analysis, and Figure 3 is a diagram showing the results of GABA quantitative analysis from the feed additive extract.

And Figure 4 is a diagram showing the intestinal bacteria classification barplot of black soldier fly larvae in a state without MSG and in a state with MSG.

Feed additives are added to compounded feed to improve digestion, reduce pathogens, improve fat metabolism, antioxidant effect, and remove toxic substances. Technically, they meet needs such as product development using natural extracts, low-cost products, and complex functional customized additives. Accordingly, the demand for technological development of related products is increasing.

In particular, insects are a high-quality alternative food resource rich in proteins, minerals, and fat, have high ecological efficiency, and emit very little greenhouse gases that cause global warming. As a future food resource that is beneficial to the environment, insects are being developed as food and functional materials. It is expected to be the most economical and sustainable way to solve the Earth's food supply problem. In particular, feed additives manufactured using these insects not only supplement nutritional components such as protein that are lacking in existing feed, but also have the functional effects mentioned above. .

In the present invention, black soldier flies are bred to contain high GABA content, and black soldier flies high in GABA content are dried with hot air and manufactured as a feed additive to improve stress and sleep disorders in livestock, farmed fish, and companion animals. do.

Dongae itself contains active ingredients such as lauric acid, a type of MCT (medium-chain triglyceride), unsaturated fatty acids, essential amino acids, peptides, chitin, and high protein, but it is used to treat stress and sleep disorders in animals. Bioconversion is required to improve .

In the present invention, it is possible to produce a feed additive for black soldier flies containing high GABA by converting glutamic acid, an amino acid, into GABA using intestinal lactic acid bacteria present in the intestines of black soldier fly larvae.

GABA is a four-carbon, non-protein amino acid inhibitory neurotransmitter. In humans, it is mostly present in the bone marrow of the brain and has physiological effects such as increasing the neurotransmitter acetylcholine and promoting brain function.

Known benefits of GABA include preventing stroke and dementia, strengthening concentration, improving memory, and relieving insomnia and depression. In addition, it has been reported to have a calming effect on nerves, relieve hangovers, relieve anxiety, lower blood pressure, and increase insulin effect. Recently, it has been attracting attention as it has been found to have anti-cancer effects, and especially when consumed by livestock, farmed fish, and companion animals, it has the effect of improving anti-stress effects and sleep disorders through its nervous stabilizing effect.

Therefore, in the present invention, 4-day-old black soldier fly larvae are fed MGS (Mono Sodium Glutamate), a glutamic acid, to contain high levels of GABA through highly efficient bioconversion ability. This glutamic acid, MSG, is used to increase GABA in the body of black soldier fly larvae. It is promoted to have a high content.

The black soldier fly larvae may be fed MSG, which is glutamic acid, directly, or mixed with soybean oil meal, rice bran oil, and soybean oil meal, and fed with MSG.

Here, MSG is for GABA conversion, and soybean oil meal, rice bran oil, and soybean oil meal are proteins, so that black soldier fly larvae consume protein, so that when the feed additive of the present invention is added to the feed in the future, not only the GABA component but also the protein component. It is possible to add it in high amounts.

MSG is contained in 5 to 15% by weight in the food of black soldier fly larvae, and soybean oil meal, rice bran oil and soybean oil meal each contain 5 to 15% by weight. The 5 to 15% by weight content of MSG has GABA bioconversion ability. It is the most economical ratio while maximizing it, and it is also the most appropriate and economical ratio for soybean oil cake, rice bran oil, and soybean oil cake.

Therefore, as shown in Figure 1, when the larvae are fed with MSG or a mixture of MSG and soybean oil, rice bran oil, and soybean oil, and fed and reared for 10 to 14 days, and then selected and dried with hot air, 1 g of larvae is added to the larvae. Contains 5 to 10 mg of GABA per serving.

Therefore, black soldier fly larvae containing GABA, GABA or high in protein can be mixed into feed as is, or processed into feed additives through a process such as powdering and mixing with other active ingredients, and then added to feed and fed to animals. There will be.

GABA qualitative analysis was performed using thin-layer chromatography (TLC). As shown in Figure 2, when ethanol is used as the extraction solvent, GABA cannot be confirmed in the extract of the feed additive, and when hot water is used as the extraction solvent, GABA cannot be confirmed. It can be seen that is extracted.

GABA quantitative analysis was performed by quantitative analysis using PITC (phenylisothiocyanate) derivatization. After putting 10 ㎕ of the feed additive extract into a tube, the solvent was removed through a vacuum concentrator, and 20 ㎕ of ethanol-water-triethylamine (TEA) (2:2:1, v/v/v) solvent was added to the residue. was added and dissolved, and the solvent was removed again through a vacuum concentrator.

Add 50 ㎕ of ethanol-water-TEA-PITC (7:1:1:1, v/v/v/v) solvent to the residue and perform PITC derivatization at room temperature for 20 minutes, followed by excess PITC derivatization. The reagent was removed through a vacuum concentrator, dissolved in 600 ㎕ solvent A, and then HPLC analysis was performed, and the results are shown in Figure 3.

Accordingly, it was confirmed that 5 to 10 mg of GABA was contained in 1 g of feed additive produced by black soldier fly larvae.

Table 1 shows the GABA content in black soldier fly larvae according to MSG content.

Drying method MSG concentration
%(w/v) GABA concentration
(mg/mL) extract volume
(mL) Total GABA content in sample GABA content
(mg/g) hot air dry 0 0.000 10 0.000 0.00 5 0.132 2.35 1.91 10 0.178 4.74 2.15 15 0.366 5.55 3.39

As shown in Table 1, the analysis results of hot air dried samples showed that the GABA content concentration was 0.000 in samples without MSG added, and 0.000, 0.132, 0.178, and 0.366 in samples with 5%, 10%, and 15% of MSG added, respectively. It was confirmed that it contained mg/mL of GABA.

Through this, it was confirmed that MSG fed to black soldier fly larvae acts as the most important factor in GABA production, and it was indirectly proven that this is the result of bioconversion by lactic acid bacteria growing in the intestines of black soldier fly larvae.

In addition, as a result of feeding MSG at different concentrations, it was confirmed that GABA production did not increase linearly with MSG concentration, so even if high concentrations of MSG were fed to black soldier fly larvae, they did not produce high concentrations of GABA, making it the most efficient and economical method. It can be seen that the appropriate amount of MSG fed is 5 to 15% by weight when 100% by weight of larvae are fed to black soldier flies.

Figure 4 is a barplot showing the classification of intestinal bacteria of black soldier fly larvae in a state without MSG and with MSG, showing that the intestinal lactic acid bacteria increased when MSG was fed compared to when MSG was not fed. This shows that a high amount of GABA can be produced through the bioconversion ability of intestinal lactic acid bacteria.

Meanwhile, in order to manufacture feed additives by feeding MSG to black soldier fly larvae to contain a high content of GABA through bioconversion, food waste can be used as food for black soldier fly larvae. In addition, grains, vegetables and agricultural products can be used. It may include at least one selected from the group consisting of by-products.

The grains, vegetables, and agricultural by-products are not particularly limited as long as they are edible for larvae of black soldier flies, etc., and those that are usually added during feed production can be used.

However, preferably, by using food waste as feed, the production cost of feed additives can be lowered and economic efficiency can be improved. In addition, there is an advantage to the environment by processing food waste and using fecal soil containing excrement as fertilizer.

Therefore, by fermenting food waste and feeding it to black soldier fly larvae, the intestinal lactic acid bacteria are increased and further activated to maximize the ability to decompose food, as well as to increase bioconversion ability to contain high GABA.

1) Strain culture

For food waste fermentation, the strains contained are selected and cultured, then mixed with food waste and fermented. The strain is Limosilactobacillus reuteri subsp. reuteri (KCTC 3594) and Lactiplantibacillus plantarum subsp. Plantarum (KCTC 3108) is used, and this strain can be purchased and used from the Biological Resource Center (Korean Collection for Type Cultures: KCTC).

Therefore, the Limosilactobacillus reuteri subsp. reuteri and Lactiplantibacillus plantarum subsp. Plantarum strains are cultured for 2 days in a culture medium with an atmosphere of 37°C.

These Limosilactobacillus reuteri subsp. reuteri (KCTC 3594) and Lactiplantibacillus plantarum subsp. Plantarum (KCTC 3108) acts as a lactic acid bacterium in the large intestine of black soldier fly larvae and bioconverts MSG into GABA.

2) Drying and powdering food waste

In the case of using the food waste as food, by removing the salt of the food waste, it is possible to later use the fecal soil composed of excrement from black soldier fly larvae as fertilizer.

At this time, in order to use fecal soil as fertilizer, the salt content in fecal soil must be less than 1%, but if food waste is used as food, due to the high salt content, the excrement that black soldier fly larvae excrete after ingesting it will also have a high salt content. .

Due to this high salt content, fecal soil made of excrement inevitably also contains high salt, so it cannot be used as fertilizer.

Therefore, food waste is sprayed with water to wash it so that the salt content is less than 1%, and then placed in a dehydrator to dehydrate. The dehydrated food waste is dried at room temperature or with hot air to have a moisture content of less than 15%, and then dried to have a moisture content of less than 15%. It is powdered by a grinder.

3) Mixing strains and dry food waste powder

The strain cultured above is mixed with the dried food waste powder. Two types of strains are mixed in the range of 1 to 5% by weight based on 100% by weight of the dried food powder, and additionally all sugar solutions (molasses) are added. This sugar solution is added to 30 to 50 ppm.

The added sugar solution is used as food for strains, and when black soldier fly larvae consume the feed additive of the present invention, it is used as food for lactic acid bacteria in the intestines, so the GABA bioconversion ability can be greatly improved by the active activity of lactic acid bacteria. .

4) Mix of MSG, Daewoo oil cake, rice bran oil cake, and soybean oil cake

In the above, 5% by weight of MSG is mixed with 100% by weight of the mixture of the strain and dried food waste powder, and if necessary, 5% by weight each of soybean oil cake, rice bran oil meal, and rapeseed oil meal, which are protein agents, are added to form Dongae. It produces food for larvae on its back.

5) Feed to black soldier fly larvae

The prepared food is fed to black soldier fly larvae and fed for 10 to 14 days, which is the most economical breeding period as a high amount of GABA is produced in the intestines of black soldier fly larvae.

Of course, this breeding period can be shortened or extended longer, but if the period is shortened, a high content of GABA may not be sufficiently produced, and if the period is prolonged, it may become uneconomical due to unnecessary increase in breeding period. Therefore, a breeding period of 10 to 14 days would be most desirable.

When reared black soldier fly larvae are selected and washed, then hot-air dried and pulverized, the powdered black soldier fly larvae have a high content of GABA of 5 to 10 mg/g and are used as a feed additive with a high content of protein. It is manufactured.

These feed additives can be added to feed as a powder itself, or alternatively, they can be manufactured in solidified pill form and added to feed.

The feed additive manufactured by the present invention has the functionality to improve stress and sleep disorders caused by long-term dense farming by being added to the feed of livestock such as cattle, pigs, chickens, and ducks, farmed fish, and companion animals. By significantly lowering the mortality rate of animals, it can increase the income of livestock farms or fish farms, and can also improve stress and sleep disorders in companion animals.

This patent was research conducted with support from the 2021 regional demand-tailored research and development project of Jeollanam-do and Jeonnam Techno Park.

[Ministry name] Jeollanam-do

[Research Management Professional Institution] Jeonnam Techno Park

[Research project name] Research and development project tailored to local demand

[Research project title] Development of high value-added functional feed additive industrialization technology using black soldier fly

[Host organization] Greenest

[Research period] 2021. 8. 1 ~ 2022. 7. 31.

[Contribution rate] 10/10

As the specific parts of the present invention have been described in detail above, it is clear to those skilled in the art that these specific techniques are merely preferred embodiments and do not limit the scope of the present invention. something to do.

Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (4)

A functional feed additive produced by feeding MGS (Mono Sodium Glutamate) to black soldier fly larvae 4 days after hatching to ensure high GABA content, and then drying the larvae on black soldier fly larvae containing GABA.
Limosilactobacillus reuteri subsp. reuteri and Lactiplantibacillus plantarum subsp. Process of culturing Plantarum strains at 37°C for 2 days;
A process of mixing the cultured strain with dry food waste powder;
A process of preparing food by mixing MGS (Mono Sodium Glutamate) with the mixture of the strain and dried food waste powder and adding soybean oil meal, rice bran oil meal, and rapeseed oil meal, respectively;
The process of feeding black soldier fly larvae 4 days after hatching with the above food and rearing them for 10 to 14 days;
A method for producing a functional feed additive, comprising the process of selecting larvae from reared black soldier flies and then drying them with hot air.
The method of claim 2, wherein the food waste is dried and powdered after removing salt.
The method of claim 2, wherein molasses is further added to the mixture of the strain and dried food waste powder to a concentration of 30 to 50 ppm.