KR20230168110A - Method for preventing hair loss using protein inside of hair follicle - Google Patents

Abstract

The present invention relates to a method for preventing hair loss using proteins in hair follicles. Specifically, the protein produced in the hair follicles reacts with the capsule layer coated with a slightly acidic modified emulsifier to form a gel, thereby filling the hair follicles with neutralized proteins to protect the hair roots. By holding the hair, it strengthens the support that prevents the hair from falling out of the hair follicle, thereby preventing hair loss. Additionally, various nutrients contained inside the capsule layer are absorbed into the skin inside the hair follicle, improving the elasticity and strength of the skin inside the hair follicle, thereby strengthening the hair root. Its characteristic is that it strengthens the hair and supplies nutrients to the hair, making the hair thicker and stronger and preventing hair loss.

Description

Method for preventing hair loss using protein inside of hair follicle}

The present invention relates to a method for preventing hair loss using proteins in hair follicles. Specifically, the protein produced in the hair follicles reacts with the capsule layer coated with a slightly acidic modified emulsifier to form a gel, thereby filling the hair follicles with neutralized proteins to protect the hair roots. By holding the hair, it strengthens the support that prevents the hair from falling out of the hair follicle, thereby preventing hair loss. Additionally, various nutrients contained inside the capsule layer are absorbed into the skin inside the hair follicle, improving the elasticity and strength of the skin inside the hair follicle, thereby strengthening the hair root. This is about a hair loss prevention method using protein in the hair follicles, which strengthens the hair and supplies nutrients to the hair, making the hair thicker and stronger and preventing hair loss.

Alopecia is influenced by various factors: genetic, hormonal and environmental factors, diet and physical activity. Hair has an essential aesthetic identity role. Therefore, alopecia in women or men can be a cause of significant psychological distress.

Therefore, healthy, strong hair and thick hair throughout life are the aspirations of most women and men. Many techniques are known for the treatment of alopecia, such as cell therapy, laser therapy or otherwise non-surgical implants. To obtain implants, human hair follicles are obtained by culturing the various cell types present in the hair bulb.

A hair follicle refers to a bag-shaped epithelial tissue that surrounds the hair root, and the hair root refers to the part where the hair is embedded in the skin. Hair grows through active cell division near the hair follicle. If this cell division ability is reduced, hair follicles atrophy and move to shallower areas of the skin. Normally, a group of new cells created by reacquiring cell division ability moves deep into the skin, forming new hair follicles, growing hair and pushing out old hair, but if this 'hair exchange' does not occur, alopecia occurs. .

The optimal treatment method for hair loss that has been developed so far is hair transplantation, but in the early stages of hair loss, treatments such as Minoxidil, a commercially available topical medication approved by the FDA, or Propecia, whose main ingredient is finasteride, an oral medication, are used to prevent hair loss. It can slow down progress.

In order to find effective substances useful in the treatment of early hair loss, the hair growth phenomenon was observed after hair removal on rats and the sample was applied to the back. In vitro organ culture using rat mustache was used to identify IGF (insulin-like growth factor), EGF. Animal experiments have been conducted to check the effects of growth factors such as Epidermal growth factor (Epidermal growth factor) and HGF (Hepatocyte growth factor), but unlike rodents, which shed their hair at regular intervals, humans have a separate cycle for each hair follicle, so there is no tendency for growth factors to be affected. All of this did not match. On the other hand, the monkey model has similar tendencies to humans, but the burden of time and experiment cost is too great. In addition, as in vivo testing using animals has recently been socially criticized and legally regulated for animal protection purposes, the development of ex vivo toxicity testing models to replace animal testing has emerged as a global issue.

Unlike in vivo experiments, in the case of in vitro experiments, long-term experiments are difficult because the supply of nutrients and oxygen through blood vessels is blocked during cell culture. In addition, rapid cell death of artificial tissue may lead to the termination of the experiment before the effect of the effective ingredient can be confirmed. Therefore, in the case of in vitro experiments, it is most important to create an environment similar to that of the living body and maintain the function of the artificial tissue for a long period of time. .

As an in vitro hair growth model using hair follicle organ culture, 10% by weight of serum was added to DMEM (Dulbecco's Modified Eagle Medium) through immersion culture and then cultured in a glass bottle. Oxygen pressure was increased and cultured in RPMI 1640 medium at 31°C. In this case, the growth rate and amount of length increase are reduced compared to culturing at 37°C, which is the general culture temperature, but there are some cases where the morphological change of the hair bulb is less.

Meanwhile, conventionally, various types of anti-hair loss and hair growth agents are generally combined drugs applied to the scalp, and have been mainly used for the purpose of promoting blood circulation in the scalp, activating hair hair cells, suppressing lipid secretion in the scalp, and supplying nutrients to the hair. .

However, the current situation is that without exception, there is almost no hair growth effect, and it seems that users are not aware of the effect of hair growth agents even though they are called hair growth agents, but have expectations just in case.

Furthermore, the use of an inappropriate concentration of hormones causes inflammation in the skin, and in some cases, hair loss occurs as well as hair loss due to the irritation of the alcohol mixed with it.

Republic of Korea Patent Publication No. 1999-0078804 Republic of Korea Public Patent No. 2000-0024499

The present invention was devised in consideration of the above-described conventional problems.

First, when applied to the scalp, the capsule layer made of a weakly acidic modified emulsifier reacts with proteins and dead skin cells formed on the surface of the scalp, making it possible to easily remove dead skin cells from the scalp and keep the scalp clean.

Second, the protein produced in the hair follicle reacts with the capsule layer coated with an emulsifier to form a gel, filling the hair follicle and holding the hair root, reinforcing the support that prevents hair from falling out of the hair follicle, thereby preventing hair loss.

Third, various nutrients contained within the capsule layer are absorbed into the epidermis of the scalp and hair follicles, improving the elasticity and strength of the skin inside the scalp and hair follicles, thereby strengthening the hair follicles and supplying nutrients to the hair, making the hair thicker and stronger, preventing hair loss. The goal is to provide a hair loss prevention method using proteins in hair follicles.

The purpose of the present invention is to prevent hair loss, in which, when an anti-hair loss composition is added into a hair follicle, the protein in the hair follicle reacts with the slightly acidic modified anti-hair loss composition supplied to the hair follicle, and the hair follicle is filled with a gel-like reactant to prevent hair loss. It is achieved by a hair loss prevention method using proteins in hair follicles, which is characterized by:

The hair loss prevention composition is composed of a mixture of two or more of the following: caffeine extract used as a base, a hair promoting extract with hair promoting ingredients, a skin irritation relieving extract with a skin irritation relieving ingredient, and a skin regenerating extract with a skin regenerating effect. It is achieved by a hair loss prevention method using proteins in hair follicles, which is achieved by reforming the mixed nutrient extract into nano-size and then mixing it with a slightly acidic modified emulsifier.

It is characterized by a mixture of 40-10% by weight of caffeine extract, 35-45% by weight of hair promotion extract, 10-17% by weight of skin irritation relief extract, and 15-23% by weight of skin regeneration extract based on 100% by weight of the mixed nutritional extract. It is achieved by a hair loss prevention method.

The emulsifier is achieved by a hair loss prevention method using a protein in hair follicles, wherein the emulsifier is one of lecithin, sorbitan, glyceryl stearate, and phospholipid.

The emulsifier is achieved by a hair loss prevention method using protein in hair follicles, which is characterized in that 2.0 to 5.0% by weight of the emulsifier is mixed.

This is achieved by a hair loss prevention method using proteins in hair follicles, wherein the gel-shaped reaction product has a viscosity in the range of 1,200 to 4,400 cps.

The fermentation process is achieved by a hair loss prevention method using proteins in hair follicles, which is characterized by mixing 10 to 20% by weight of fermenting bacteria with 100% by weight of the extract and fermenting at a temperature of 35 to 40 ° C. for 24 to 72 hours. .

This invention is

First, when applied to the scalp, the capsule layer made of emulsifier reacts with proteins and dead skin cells on the surface of the scalp, making it possible to easily remove dead skin cells from the scalp and keep the scalp clean.

Second, the protein produced in the hair follicle reacts with the capsule layer coated with an emulsifier to form a gel, filling the hair follicle and holding the hair root, reinforcing the support that prevents hair from falling out of the hair follicle, thereby preventing hair loss.

Third, various nutrients contained within the capsule layer are absorbed into the scalp and the epidermis inside the hair follicles, improving the elasticity and strength of the skin inside the scalp and hair follicles, thereby strengthening the hair follicles and supplying nutrients to the hair, making the hair thicker and stronger, preventing hair loss. It is a very useful invention that has the following effects:

1 is a manufacturing process diagram showing a method of manufacturing an anti-hair loss composition to which the technology of the present invention is applied.
Figure 2 is a photograph showing the reaction between the anti-hair loss composition prepared according to the present invention and the protein.

Hereinafter, a preferred embodiment of the present invention will be described in detail based on the attached drawings.

The attached drawing, Figure 1, is a manufacturing process diagram showing a method of manufacturing a hair loss prevention composition using protein in hair follicles to which the technology of the present invention is applied. The present invention extracts caffeine, which has skin cancer prevention, hair growth promotion, and moisturizing effects, from natural plants. Caffeine extract used as a base, a hair promoting extract extracted from the first natural plant group with hair promoting ingredients, a skin irritation relieving extract extracted from the second natural plant group with skin irritation relieving ingredients, and a skin regenerating effect. A raw material preparation process (S100) is performed to prepare a mixed nutritional extract by mixing skin regeneration extracts extracted from the third natural plant group.

The raw material preparation process (S100) consists of natural plants from which caffeine can be extracted, the first natural plant group consisting of goji berry, licorice, dermis, jujube, perilla leaves, ginseng, black beans, Houttuynia cordata, green tea, and green tea. , the second natural flora group consisting of ginkgo leaves, persimmon leaves, Cornus officinalis fruit, dandelion, perilla leaves, deodeok, sycamore, mulberry fruit, bellflower root, licorice, etc., and thistle, Schisandra chinensis, dermis, jujube, brown rice, bulb root, kelp, etc. The third natural plant group is thoroughly washed and dried at 60°C for 24 to 48 hours, then cut to a certain size and dried. Then, each natural plant and natural plant group is placed in a clean burlap bag and re-dried for about 24 hours. Place the burlap bag in a container prepared with distilled water or water and heat it. At this time, the extract is collected by collecting the water vapor generated.

At this time, based on 100% by weight of the mixed nutritional extract, 40-10% by weight of caffeine extract, 35-45% by weight of hair promotion extract, 10-17% by weight of skin irritation relief extract, and 15-23% by weight of skin regeneration extract are mixed. It has that characteristic.

The reason why the extraction method used in the raw material preparation process (S100) is limited to the steam extraction method is because no residual solvent is generated, there is no difficulty in selecting a solvent, and the extraction method is simple, low-cost, and highly efficient.

Meanwhile, a fermentation process (S200) is performed to ferment the mixed nutrient extract by adding Bacillus fermentation strain to the mixed nutrient extract prepared by mixing each extract obtained through the raw material preparation process (S100).

In the fermentation process (S200) of fermenting the mixed nutrient extract, 10 to 20 wt% of fermenting bacteria are mixed with 100 wt% of the extract and fermented at a temperature of 35 to 40 ° C. for 24 to 72 hours.

The reason for fermenting the mixed nutrient extract as described above is to change the mixed nutrient extract with a high molecular weight matrix into a low molecular weight substrate to facilitate absorption into the scalp (skin) and improve antibacterial activity. The degree of irritation, hair elasticity, and scalp improvement can be found in Table 1 below.

[Experiment 1]

<Experimental example>

100 ml of mixed nutritional extract was prepared by mixing 25.0% by weight of caffeine extract, 40.0% by weight of hair promotion extract, 15.0% by weight of skin irritation relief extract, and 20.0% by weight of skin regeneration extract.

<Comparative Example 1>

The unfermented mixed nutrient extract was tested (5-point measurement method) on scalp (skin) absorption, scalp (skin) irritation, and hair elasticity by a total of 30 people (15 men and 15 women). The level of scalp improvement was measured every 5 days for 30 days using a portable scalp measuring device.

<Example 1>

100 ml of the mixed nutrient extract was prepared by fermenting the mixed nutrient extract, and a total of 30 people (15 men and 15 women) were surveyed to evaluate the sensory scalp (skin) absorption capacity, scalp (skin) irritation, and hair elasticity. It is shown in Table 1 below. The level of scalp improvement was measured every 5 days for 30 days using a portable scalp measuring device.

scalp absorption capacity Scalp irritation hair elasticity Scalp improvement Comparative Example 1
Unfermented mixed nutritional extract (conventional) Very little A bit irritating commonly 4.13 Example 1
Fermented mixed nutritional extract (present invention) speed no irritation good 4.82

Experiment method: Disk Diffusion method

By the fermentation process (S200) of fermenting the mixed nutrient extract obtained by the raw material preparation process (S100), a high-concentration fermentation extract is produced by centrifuging the filtered water separated and purified by adsorbing the bacteria and foreign substances mixed in the fermentation broth. Perform the high-concentration fermentation extract acquisition process (S300).

The high-concentration fermentation extract acquisition process (S300) removes foreign substances from the fermentation broth after the fermentation process (S200) using a slightly acidic ion exchange resin. Additionally, the fermentation broth that has passed through the ion exchange resin is placed in a centrifuge and centrifuged to complete the previous process. Phase separation occurs at the bottom for particulate foreign matter and bacterial cells that cannot be removed, and for the solution containing the active ingredient at the top. The supernatant is separated in a centrifuge where phase separation occurred and stored and aged in a container for 1 to 2 days to achieve highly concentrated mixing. Obtain nutritional extract.

A nanoization process (S400) is performed to safely and stably deliver the high-concentration mixed nutrient extract active ingredients obtained as above to the dermal layer of the skin and manufacture them in a size of 10 to 50 nm to facilitate skin absorption.

In the nanoization process (S400), the mixed nutrient extract is brought to a temperature of 40 to 50°C, then the mixed nutrient extract having the above temperature is placed in a pad mixer, stirred slowly at 60 to 100 rpm, and mixed at 2,500 to 3,500 rpm using a homomixer. Stir for about 2 to 4 minutes, and when the stirring is completed, the process is repeated 3 times at a pressure of 700 to 1,000 bar in a microfluidizer.

Meanwhile, as shown in Figure 2 of the attached drawing, looking at the difference in skin absorption between the mixed nutrient extract without the nanoization process (S400) and the mixed nutrient extract that underwent the nanoization process (S400), the nanoization process (S400) as in the present invention It can be seen that the skin absorption capacity of the mixed nutritional extract was significantly higher.

A mixed encapsulation process (S500) is performed to encapsulate the high-concentration mixed nutrient extract for which the nanoization process (S400) has been completed by mixing a weakly acidic emulsifier. When mixing the emulsifier, if the emulsifier does not exhibit a pH range of 5.5 to 6.5, it is preferable to add a pH adjuster to adjust the acidity of the emulsifier to make it slightly acidic. The reason is that if the pH is below 5.5, there is a risk of damage to hair and skin, and if the pH is above 6.5, there is a problem in that it cannot react with the protein and does not change state into a gel.

As shown in Figure 2, the protein in the hair follicle reacts with the capsule layer coated with an emulsifier to form a gel, showing that the inside of the hair follicle is filled with neutralized protein. At this time, the neutralized protein holds the hair root and forms a substance inside the capsule layer. Various nutrients are absorbed into the skin inside the hair follicles, improving the elasticity and strength of the skin inside the hair follicles, thereby strengthening the hair roots, and supplying nutrients to the hair, making the hair thicker and stronger.

Meanwhile, in the mixed encapsulation process (S500), the emulsifier uses lecithin extracted from soybeans, eggs, etc., and instead of lecithin, sorbitan, glyceryl stearate, and phospholipid ( Phospolipid) can be used.

During the mixed encapsulation process (S500), when 2.0 to 5.0% by weight of an emulsifier is added to 100% by weight of the high-concentration fermented extract, the emulsifier forms a coating layer, that is, a capsule, on the surface, and the inside of the capsule made of the emulsifier contains a high concentration of emulsifier. Contains mixed nutritional extract.

If the emulsifier is used in an amount of 2.0% by weight or less, the gel formed by the reaction between the protein in the hair follicle and the capsule layer coated with the emulsifier does not form sufficient viscosity and is in an unstable state, so it does not function properly as an emulsifier and separation phenomenon may occur. There is a possibility that if more than 5.0% by weight of the above emulsifier is used, the viscosity of the gel formed by the reaction between the protein in the hair follicle and the capsule layer coated with the emulsifier becomes high, exceeding the appropriate range, and the emulsifiers clump together to form uneven crystals. Because there is a problem.

A dual liposome process (S600) is performed to nanosize the encapsulated mixture again. The dual liposome process (S600) is one more process similar to the nanoization process (S400), and the process description is omitted. It is manufactured with a molecular size of 50 to 120 nm. The structure at this time is an extract of natural ingredients with good skin compatibility. It has a structure coated with a double layer.

The reason for encapsulating the mixed nutrient extract as described above is to ensure that the mixed nutrient extract can be easily supplied into the hair follicles when the mixed nutrient extract is applied or sprayed to the scalp, and the anti-hair loss composition of the present invention into the hair follicles. Table 3 below shows an experiment that shows changes in the hair follicles when this product enters the hair follicles, and Table 4 shows the protein reaction rate according to the acidity of the emulsifier.

<Comparative Example 2>

100 ml of the unfermented mixed nutrient extract (Comparative Example 1) was subjected to nano-processing to prepare 100 ml of the mixed nutrient extract, and 2 ml of protein peptide was mixed with the nano-formed un-fermented mixed nutrient extract, and the reaction over time was observed.

<Example 2>

100 ml of high-concentration fermentation extract that underwent nano-processing was mixed with 2 ml of emulsifier at pH 5.5 and 2 ml of protein peptide was mixed, and the reaction over time was observed.

<Example 3>

2 ml of emulsifier of pH 6.0 was mixed with 100 ml of high-concentration fermentation extract that underwent nano-processing, and the dual liposome process was performed. After mixing 2 ml of protein peptide, the viscosity was examined over time.

1 minute passed
(Viscosity or photo) 2 minutes passed 3 minutes passed 5 minutes passed Comparative Example 2 1,200 cps 1,250 cps 1,500 cps 1,900 cps Example 2 1,300 cps 1,500 cps 1,900 cps 2,600 cps Example 3 1,600 cps 2,200 cps 3,000 cps 4,200 cps

Experiment: Brookfield DV2TL VTJO Spindle: 62, Speed: 30rpm, Temperature: 25℃, Time: 30sec

Acidity of emulsifier Tensile force when the hair is pulled after reaction pH 5.5 pH 5.8 pH 6.0 pH 6.5 Comparative Example 2 1,750 cps 1,900 cps 1,900 cps 2,200 cps commonly Example 2 2,200 cps 2,500 cps 2,600 cps 2,900 cps strong Example 3 3,500 cps 4,100 cps 4,200 cps 4,400 cps very strong

Experiment: Measured after aging for 72 hours in a constant temperature bath at 25℃ Brookfield DV2TL VTJO Spindle: 62, Speed: 30rpm, Temperature:

25℃, Time: 30sec

When the emulsifier and protein react, the viscosity of the gel-shaped reaction product is preferably in the range of 1,200 to 4,400 cps. If the viscosity is less than 1,200 cps, the viscosity of the gel formed by the reaction between the protein in the hair follicle and the capsule layer coated with the emulsifier is too low. There is a problem in preventing hair loss due to low gel formation and lack of strength to hold the hair roots.

On the other hand, if the viscosity is more than 4,400cps, the viscosity of the gel formed by the reaction between the protein in the hair follicle and the capsule layer coated with the emulsifier is too high, and the gel filled in the hair follicle may block the hair follicle, causing scalp trouble. Various nutrients are not sufficiently absorbed into the scalp and hair, so they do not function as an anti-hair loss effect.

When the present invention is applied to the scalp, the capsule layer made of an emulsifier reacts with the proteins and keratin formed on the surface of the scalp, making it possible to easily remove dead skin cells from the scalp, so that the scalp can be kept clean, and the hair follicles formed in the hair follicles can be easily removed. As the capsule layer coated with protein and emulsifier reacts and becomes gel-like, it fills the hair follicle and holds the hair root, reinforcing the support that prevents hair from falling out of the hair follicle, thereby preventing hair loss. The inside of the capsule layer is on the scalp and the epidermis inside the hair follicle. It is a very useful invention that has the effect of absorbing various nutrients provided in the hair follicles, improving the elasticity and strength of the scalp and the skin inside the hair follicles, thereby strengthening the hair follicles, supplying nutrients to the hair, making the hair thicker and stronger, and preventing hair loss. .

Claims (3)

In the hair loss prevention method,
When an anti-hair loss composition is placed into a hair follicle, the anti-hair loss composition, which is made by mixing the protein in the hair follicle and a slightly acidic modified emulsifier supplied to the hair follicle, reacts and fills the hair follicle with a gel-like reactant to prevent hair loss. A hair loss prevention method using the characteristic hair follicle protein. According to claim 1,
The hair loss prevention composition is composed of a mixture of two or more of the following: caffeine extract used as a base, a hair promoting extract with hair promoting ingredients, a skin irritation relieving extract with a skin irritation relieving ingredient, and a skin regenerating extract with a skin regenerating effect. A hair loss prevention method using proteins in hair follicles, characterized in that the mixed nutrient extract is lipolyzed to nano size and then mixed with a slightly acidic modified emulsifier. The method of claim 1 or 2,
A hair loss prevention method using protein in hair follicles, characterized in that the emulsifier is one of lecithin, sorbitan, and glyceryl stearate modified to be slightly acidic in the pH range of 5.5 to 6.5.