KR20230158358A - Manufacturing Method for Livestock Pellet Feed - Google Patents
Manufacturing Method for Livestock Pellet Feed Download PDFInfo
- Publication number
- KR20230158358A KR20230158358A KR1020220058033A KR20220058033A KR20230158358A KR 20230158358 A KR20230158358 A KR 20230158358A KR 1020220058033 A KR1020220058033 A KR 1020220058033A KR 20220058033 A KR20220058033 A KR 20220058033A KR 20230158358 A KR20230158358 A KR 20230158358A
- Authority
- KR
- South Korea
- Prior art keywords
- weight
- powder
- lactic acid
- acid bacteria
- feed
- Prior art date
Links
- 239000008188 pellet Substances 0.000 title claims abstract description 30
- 244000144972 livestock Species 0.000 title claims abstract description 24
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 16
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 66
- 239000000843 powder Substances 0.000 claims abstract description 51
- 241000894006 Bacteria Species 0.000 claims abstract description 33
- 239000004310 lactic acid Substances 0.000 claims abstract description 33
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 33
- 239000000203 mixture Substances 0.000 claims abstract description 24
- 238000000855 fermentation Methods 0.000 claims abstract description 15
- 230000004151 fermentation Effects 0.000 claims abstract description 15
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 14
- 239000011259 mixed solution Substances 0.000 claims abstract description 10
- 241000121220 Tricholoma matsutake Species 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 238000000748 compression moulding Methods 0.000 claims abstract description 4
- 241000207199 Citrus Species 0.000 claims description 18
- 235000020971 citrus fruits Nutrition 0.000 claims description 18
- 244000302512 Momordica charantia Species 0.000 claims description 13
- 235000009811 Momordica charantia Nutrition 0.000 claims description 13
- 239000000284 extract Substances 0.000 claims description 12
- 240000005979 Hordeum vulgare Species 0.000 claims description 10
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 10
- 235000013339 cereals Nutrition 0.000 claims description 9
- 235000013399 edible fruits Nutrition 0.000 claims description 9
- 239000006227 byproduct Substances 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 235000012054 meals Nutrition 0.000 claims description 5
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 16
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 15
- 239000003242 anti bacterial agent Substances 0.000 description 9
- 229940088710 antibiotic agent Drugs 0.000 description 9
- 239000004615 ingredient Substances 0.000 description 9
- 239000002609 medium Substances 0.000 description 9
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 8
- 229930003268 Vitamin C Natural products 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 235000002639 sodium chloride Nutrition 0.000 description 8
- 235000019154 vitamin C Nutrition 0.000 description 8
- 239000011718 vitamin C Substances 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 239000001888 Peptone Substances 0.000 description 7
- 108010080698 Peptones Proteins 0.000 description 7
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 7
- 229940041514 candida albicans extract Drugs 0.000 description 7
- 239000008103 glucose Substances 0.000 description 7
- 235000019319 peptone Nutrition 0.000 description 7
- 235000013406 prebiotics Nutrition 0.000 description 7
- 239000001632 sodium acetate Substances 0.000 description 7
- 235000017281 sodium acetate Nutrition 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 239000012138 yeast extract Substances 0.000 description 7
- 238000010438 heat treatment Methods 0.000 description 6
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 5
- 229920002498 Beta-glucan Polymers 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- 230000000844 anti-bacterial effect Effects 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 4
- 241000283690 Bos taurus Species 0.000 description 4
- 241000186660 Lactobacillus Species 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 239000000835 fiber Substances 0.000 description 4
- 229940039696 lactobacillus Drugs 0.000 description 4
- 235000019645 odor Nutrition 0.000 description 4
- 239000006041 probiotic Substances 0.000 description 4
- 235000018291 probiotics Nutrition 0.000 description 4
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 3
- 235000011613 Pinus brutia Nutrition 0.000 description 3
- 241000018646 Pinus brutia Species 0.000 description 3
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 240000002234 Allium sativum Species 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 235000015165 citric acid Nutrition 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 235000004611 garlic Nutrition 0.000 description 2
- 235000001727 glucose Nutrition 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000012533 medium component Substances 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 description 1
- 241000186016 Bifidobacterium bifidum Species 0.000 description 1
- 241001608472 Bifidobacterium longum Species 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 240000001046 Lactobacillus acidophilus Species 0.000 description 1
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 1
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 1
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 1
- 240000006024 Lactobacillus plantarum Species 0.000 description 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 1
- 240000000599 Lentinula edodes Species 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 235000008322 Trichosanthes cucumerina Nutrition 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229940002008 bifidobacterium bifidum Drugs 0.000 description 1
- 229940009289 bifidobacterium lactis Drugs 0.000 description 1
- 229940009291 bifidobacterium longum Drugs 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 235000010216 calcium carbonate Nutrition 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000001877 deodorizing effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- PVBRXXAAPNGWGE-LGVAUZIVSA-L disodium 5'-guanylate Chemical compound [Na+].[Na+].C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP([O-])([O-])=O)[C@@H](O)[C@H]1O PVBRXXAAPNGWGE-LGVAUZIVSA-L 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003746 feather Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 229940029982 garlic powder Drugs 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000007602 hot air drying Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 1
- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 235000018977 lysine Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 235000006109 methionine Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 235000019633 pungent taste Nutrition 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 235000021012 strawberries Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000021092 sugar substitutes Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 239000003760 tallow Substances 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 150000003700 vitamin C derivatives Chemical class 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/25—Shaping or working-up of animal feeding-stuffs by extrusion
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Animal Husbandry (AREA)
- Mycology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Birds (AREA)
- Fodder In General (AREA)
Abstract
본 발명은 볶은 귀리 분말, 참솔 송이버섯 분말, 사카로마이세스 세레비지에(Saccharomyces cerevisiae) 효모를 포함하는 혼합액을 제조하는 단계(S10); 상기 혼합액에 유산균을 접종하여 유산균 발효액을 제조하는 단계(S20); 피톤치드를 포함하는 사료조성물에 상기 유산균 발효액을 혼합하여 펠렛조성물을 제조하는 단계(S30); 상기 펠렛조성물을 압축성형 하여 펠렛사료를 제조하는 단계(S40);를 포함하는 것을 특징으로 하는 가축 펠렛사료 제조방법에 관한 것이다. The present invention includes the step of preparing a mixed solution containing roasted oat powder, Chamsol pine mushroom powder, and Saccharomyces cerevisiae yeast (S10); Preparing a lactic acid bacteria fermentation broth by inoculating the mixed solution with lactic acid bacteria (S20); Preparing a pellet composition by mixing the lactic acid bacteria fermentation broth with a feed composition containing phytoncide (S30); It relates to a method for manufacturing livestock pellet feed, comprising: manufacturing pellet feed by compression molding the pellet composition (S40).
Description
본 발명은 항생제를 사용하지 않고도 가축의 항균성 스트레스의 해소, 악취제거 등 축사 환경개선작용 등의 장점이 있고, 프로바이오틱스와 프리바이오틱스를 동시에 제공하여 효율적이며, 첨가되는 곡물 등에 의해 발효배양액에 존재하는 유산균의 안정성을 제공하는 가축 펠렛사료 제조방법에 관한 것이다. The present invention has the advantages of relieving antibacterial stress in livestock and improving the livestock environment such as removing odors without using antibiotics. It is efficient by providing probiotics and prebiotics at the same time, and it is efficient by providing probiotics and prebiotics at the same time. This relates to a method for manufacturing livestock pellet feed that provides stability to lactic acid bacteria.
축산업은 우리나라 식량산업에서 큰 부분을 차지하는 중요한 산업이지만, 생산비 중 사료비가 차지하는 비율이 크고 사료원료는 수입 의존도가 높은 상황에서 수입사료의 가격 상승은 축산업의 수익성에 큰 방해요소가 되고 있다.Livestock industry is an important industry that accounts for a large portion of Korea's food industry, but with feed costs accounting for a large proportion of production costs and a high dependence on imports for feed raw materials, the rise in the price of imported feed is a major impediment to the profitability of the livestock industry.
한편, 지난 수십 년간 우리 축산업은 가축의 예방과 치료 및 가축의 성장을 촉진하고, 사료효율을 개선하여, 가축의 생산성 향상을 기하기 위하여 보편적으로 항생제를 사용하여 왔다.Meanwhile, over the past several decades, our livestock industry has commonly used antibiotics to prevent and treat livestock, promote livestock growth, improve feed efficiency, and improve livestock productivity.
그러나 항생제를 계속 사용하게 되면 내성을 가지는 미생물이 출현하여 항생물질의 사용량을 늘려야 하거나, 항생물질 자체의 효과가 발생하지 않는 문제가 발생하며, 또한 축산물 중에 항생물질이 잔류하게 되어 이를 섭취하게 되는 인체에 심각한 부작용을 초래한다는 점에서, 성장촉진을 목적으로 한 항생제의 사용이 점차 규제 되고 있다.However, if antibiotics are continuously used, resistant microorganisms may emerge, requiring an increase in the amount of antibiotics used, or the antibiotics themselves may not be effective. In addition, antibiotics may remain in livestock products, causing human bodies to ingest them. Because they cause serious side effects, the use of antibiotics for growth promotion is gradually being regulated.
이러한 문제점을 해결하기 위한 종래 기술의 예로 대한민국 특허공개 제10-2004-0069487호에서는 옥수수, 대두박, 수지박, 우모분, 곡물부산물, 인산칼슘, 탄산칼슘, 천일염, 라이신, 메치오닌, 미네랄/비타민트리믹스를 포함하는 가금용 사료에 있어서, 마늘의 매운맛과 냄새를 제거하기 위하여 분쇄 후 열풍 건조에 의하여 건조한 0.5 내지 3 중량부의 마늘 분말을 더 포함하는 것을 특징으로 하는 마늘성분을 포함하는 가금용 사료를 제시하고 있으나, 상기 기술의 경우 가축의 항균성 스트레스의 해소, 악취제거 등 축사 환경개선작용 등의 효과를 충분히 기대하기 곤란한 단점을 갖는다.As an example of the prior art to solve these problems, Korean Patent Publication No. 10-2004-0069487 contains corn, soybean meal, tallow meal, feather meal, grain by-products, calcium phosphate, calcium carbonate, sea salt, lysine, methionine, and mineral/vitamin tree. In the poultry feed containing the mix, the poultry feed containing the garlic ingredient further comprises 0.5 to 3 parts by weight of garlic powder that is ground and dried by hot air drying to remove the pungency and odor of the garlic. However, the above technology has the disadvantage that it is difficult to fully expect effects such as relieving antibacterial stress in livestock and improving livestock environment such as removing odor.
이에 본 발명은 상술한 바와 같은 종래기술의 문제점을 해결하기 위해 안출된 것으로 항생제를 사용하지 않고도 가축의 항균성 스트레스의 해소, 악취제거 등 축사 환경개선작용 등의 장점이 있고, 프로바이오틱스와 프리바이오틱스를 동시에 제공하여 효율적이며, 첨가되는 곡물 등에 의해 발효배양액에 존재하는 유산균의 안정성을 제공하는 가축 펠렛사료 제조방법을 제공하고자 함이다. Accordingly, the present invention was developed to solve the problems of the prior art as described above, and has advantages such as relieving antibacterial stress in livestock and improving livestock environment such as removing odor without using antibiotics, and using probiotics and prebiotics. The purpose is to provide a method for manufacturing livestock pellet feed that is efficient by providing the same and provides stability to the lactic acid bacteria present in the fermentation culture due to the added grains, etc.
상기 과제를 해결하기 위한 본 발명의 가축 펠렛사료 제조방법(이하 “본 발명의 제조방법”이라함)은, 볶은 귀리 분말, 참솔 송이버섯 분말, 사카로마이세스 세레비지에(Saccharomyces cerevisiae) 효모를 포함하는 혼합액을 제조하는 단계(S10); 상기 혼합액에 유산균을 접종하여 유산균 발효액을 제조하는 단계(S20); 피톤치드를 포함하는 사료조성물에 상기 유산균 발효액을 혼합하여 펠렛조성물을 제조하는 단계(S30); 상기 펠렛조성물을 압축성형 하여 펠렛사료를 제조하는 단계(S40);를 포함하는 것을 특징으로 한다. The livestock pellet feed manufacturing method of the present invention (hereinafter referred to as the “manufacturing method of the present invention”) to solve the above problems includes roasted oat powder, pine mushroom powder, and Saccharomyces cerevisiae yeast. Preparing a mixed solution containing (S10); Preparing a lactic acid bacteria fermentation broth by inoculating the mixed solution with lactic acid bacteria (S20); Preparing a pellet composition by mixing the lactic acid bacteria fermentation broth with a feed composition containing phytoncide (S30); Compressing the pellet composition to produce pellet feed (S40).
하나의 예로 상기 사료조성물은, 옥수수분, 곡류부산물, 피톤치드, 여주추출물, 감귤박 건조분말을 포함하는 것을 특징으로 한다. As an example, the feed composition includes corn meal, grain by-products, phytoncide, bitter melon extract, and citrus peel dried powder.
하나의 예로 S10단계에는 감귤박 건조분말이 더 포함되는 것을 특징으로 한다. As an example, step S10 further includes dried citrus peel powder.
하나의 예로 S10단계에는 보리수열매 건조분말이 더 포함되는 것을 특징으로 한다. As an example, step S10 further includes dried barley fruit powder.
이상에서 설명한 바와 같이 본 발명의 제조방법에 의해 제조되는 펠렛사료는 항생제를 사용하지 않고도 소의 항균성 스트레스의 해소, 축사 환경개선작용 등의 장점이 있다. As described above, the pellet feed manufactured by the manufacturing method of the present invention has advantages such as relieving antibacterial stress in cattle and improving the livestock environment without using antibiotics.
또한 프로바이오틱스와 프리바이오틱스를 동시에 제공하여 효율적이며, 첨가되는 곡물 등에 의해 발효배양액에 존재하는 유산균의 안정성을 제공하는 장점이 있다. In addition, it is efficient because it provides both probiotics and prebiotics at the same time, and has the advantage of providing stability to the lactic acid bacteria present in the fermentation culture due to the added grains.
도 1은 본 발명의 제조방법을 나타내는 블록도이다. 1 is a block diagram showing the manufacturing method of the present invention.
이하, 본 발명의 구성 및 작용을 첨부된 도면에 의거하여 좀 더 구체적으로 설명한다. 본 발명을 설명함에 있어서, 본 명세서 및 청구범위에 사용된 용어나 단어는 발명자가 그 자신의 발명을 가장 최선의 방법으로 설명하기 위해 용어의 개념을 적절하게 정의할 수 있다는 원칙에 입각하여 본 발명의 기술적 사상에 부합하는 의미와 개념으로 해석되어야만 한다.Hereinafter, the configuration and operation of the present invention will be described in more detail based on the attached drawings. In describing the present invention, the terms and words used in the specification and claims are based on the principle that the inventor can appropriately define the concept of the term in order to explain the invention in the best way. It must be interpreted with meaning and concepts consistent with the technical ideas of.
본 발명의 제조방법은 도 1에서 보는 바와 같이 볶은 귀리 분말, 참솔 송이버섯 분말, 사카로마이세스 세레비지에(Saccharomyces cerevisiae) 효모를 포함하는 혼합액을 제조하는 단계(S10); 상기 혼합액에 유산균을 접종하여 유산균 발효액을 제조하는 단계(S20); 피톤치드를 포함하는 사료조성물에 상기 유산균 발효액을 혼합하여 펠렛조성물을 제조하는 단계(S30); 상기 펠렛조성물을 압축성형 하여 펠렛사료를 제조하는 단계(S40);를 포함하는 것을 특징으로 한다. As shown in Figure 1, the manufacturing method of the present invention includes the steps of preparing a mixed solution containing roasted oat powder, Chamsol pine mushroom powder, and Saccharomyces cerevisiae yeast (S10); Preparing a lactic acid bacteria fermentation broth by inoculating the mixed solution with lactic acid bacteria (S20); Preparing a pellet composition by mixing the lactic acid bacteria fermentation broth with a feed composition containing phytoncide (S30); Compressing the pellet composition to produce pellet feed (S40).
본 발명은 우선 볶은 귀리 분말, 참솔 송이버섯 분말, 사카로마이세스 세레비지에(Saccharomyces cerevisiae) 효모를 포함하는 혼합액을 제조하는 단계(S10)를 갖는다. The present invention first has a step (S10) of preparing a mixed solution containing roasted oat powder, Chamsol pine mushroom powder, and Saccharomyces cerevisiae yeast.
본 단계(S10)에서는 상기 조성들 외에도 유산균 배지성분이 더 첨가되는데, 예로 펩톤, 효모추출물, 포도당, 구연산, 소듐 아세테이트, 천일염이 첨가될 수 있다. In this step (S10), in addition to the above compositions, additional lactic acid bacteria medium components are added. For example, peptone, yeast extract, glucose, citric acid, sodium acetate, and sea salt may be added.
상기 볶은 귀리 분말은 프리바이오틱 성분인 베타글루칸 섬유는 물론 당질과 각종 비타민, 미네랄의 존재로 유산균의 배양성분으로 사용한다. 특히 고압멸균과정에서 호화된 당질은 유산균이 에너지원으로 용이하게 사용될 수 있는 것이다. The roasted oat powder is used as a culture ingredient for lactic acid bacteria due to the presence of beta-glucan fiber, which is a prebiotic ingredient, as well as carbohydrates and various vitamins and minerals. In particular, sugars gelatinized during the high-pressure sterilization process can be easily used by lactic acid bacteria as an energy source.
표고버섯의 일종인 참솔 송이버섯은 식용으로서의 풍미를 제공하는 아미노산인 구아닐산 나트륨과 베타글루칸을 32%를 함유하고 있는 버섯으로 유산균 배양과정에서 많은 영양성분을 공급하며, 높은 함량의 베타글루칸을 제공한다.Chamsol pine mushroom, a type of shiitake mushroom, is a mushroom that contains 32% of sodium guanylate and beta-glucan, amino acids that provide edible flavor. It supplies many nutrients during the lactic acid bacteria culture process and provides a high content of beta-glucan. .
사카로마이세스 세레비지에(Saccharomyces cerevisiae) 효모는 효모의 구성 성분 중 유산균 생장에 필요한 수용성 영양성분을 공급하고 23%의 베타글루칸 섬유를 함유하고 있어 배양 종료 후 배양액에 프리바이오틱스 성분으로 남아있게 된다. Among the yeast's components, Saccharomyces cerevisiae yeast supplies water-soluble nutrients necessary for the growth of lactic acid bacteria and contains 23% beta-glucan fiber, so it remains as a prebiotic ingredient in the culture medium after the end of cultivation. do.
또한 상기 S10단계에서는 감귤박 건조분말이 더 포함되도록 할 수 있다. Additionally, in step S10, citrus peel dried powder may be further included.
상기 감귤박 건조분말은 상기 혼합물의 당화효율을 높이기 위한 것으로 설탕대용으로 하여 최종 결과물인 유산균 발효액을 수득하는 것이다. 또한 감귤의 향과 맛이 유산균 발효액에 발현되도록 하는 것이다. The citrus peel dried powder is used as a sugar substitute to increase the saccharification efficiency of the mixture to obtain the final result, a lactic acid bacteria fermentation broth. It also allows the scent and taste of citrus to be expressed in the lactic acid bacteria fermentation liquid.
상기 감귤박이라 함은 감귤 착즙 후의 나머지 찌꺼기 부분을 말하는 것으로, 이러한 감귤박을 40 내지 60℃에서 1시간 가량 건조한 후 분쇄하여 제조하여 건조분말이 제조되도록 하는 것이다. The citrus peel refers to the remaining residue after citrus juice has been squeezed, and the citrus peel is dried at 40 to 60° C. for about 1 hour and then pulverized to produce dry powder.
상기 감귤박의 조섬유는 가축에서 소화가 잘 되는 것으로 알려져 있으며 당성분은 물론 다양한 영양성분의 에너지가는 곡류와 비슷할 정도로 높은 것으로 알려져 있다. The crude fiber of the citrus peel is known to be easily digested by livestock, and the energy value of various nutrients as well as sugar content is known to be high enough to be similar to that of grains.
또한 본 발명에서는 S10단계에는 보리수열매 건조분말이 더 포함되는 예를 제시하고 있다. In addition, the present invention provides an example in which barley fruit dried powder is further included in step S10.
상기 보리수열매 건조분말이 첨가되도록 하는 이유는 보리수열매에 탄닌성분이 함유로 인해 이를 사료로 하는 소는 물론 소를 통해 인체로 전달될 수 있는 중금속 축적억제능이 발현되도록 하는 것이다. The reason for adding the dried barley fruit powder is to express the ability to suppress the accumulation of heavy metals that can be transmitted to the human body through cattle as well as cattle that feed on barley fruit due to the tannin component contained in barley fruit.
상기 보리수열매 건조분말의 경우도 상기 감귤박 건조분말의 제조와 유사한 방법으로 제조될 수 있어 그 상세 설명은 생략한다. The barley fruit dried powder can be manufactured in a similar manner to the citrus peel dried powder, so detailed description thereof will be omitted.
바람직하게 본 단계(S10)에서는 물 100중량부에 대해 펩톤 1 내지 3중량부, 효모추출물 1 내지 3중량부, 포도당 1 내지 3중량부, 구연산 1 내지 3중량부, 소듐 아세테이트 2 내지 7중량부, 천일염 0.1 내지 1중량부, 볶은 귀리 분말 5 내지 15중량부, 참솔 송이버섯 분말 2 내지 5중량부, 사카로마이세스 세레비지에 효모 2 내지 8중량부, 감귤박 건조분말 0.5 내지 2중량부, 보리수열매 건조분말 0.5 내지 2중량부로 배합하여 혼합물을 제조하는 것이 타당하다. Preferably, in this step (S10), 1 to 3 parts by weight of peptone, 1 to 3 parts by weight of yeast extract, 1 to 3 parts by weight of glucose, 1 to 3 parts by weight of citric acid, and 2 to 7 parts by weight of sodium acetate per 100 parts by weight of water. , 0.1 to 1 part by weight of sea salt, 5 to 15 parts by weight of roasted oat powder, 2 to 5 parts by weight of Chamsol pine mushroom powder, 2 to 8 parts by weight of Saccharomyces cerevisiae yeast, 0.5 to 2 parts by weight of citrus peel dried powder. , it is appropriate to prepare a mixture by mixing 0.5 to 2 parts by weight of dried barley fruit powder.
그 다음으로 상기 혼합액에 유산균을 접종하여 유산균 발효액을 제조하는 단계(S20)를 갖는다. Next, there is a step (S20) of inoculating the mixed solution with lactic acid bacteria to prepare a lactic acid bacteria fermentation broth.
상기 유산균은 상기 혼합물 100중량부에 대해 0.1 내지 10중량부를 포함하도록 하는 것이 타당한데, 균종을 특별히 한정하지 않으며 예로 락티플란티바실러스 플란타륨(Lactiplantibacillus plantarum), 락토바실러스 애시도필러스(Lactobacillus acidophilus), 락티카제이바실러스 카제이(Lacticaseibacillus casei), 락토바실러스 불가리쿠스 (Lactobacillus bulgaricus), 비피도박테리움 비피듐(Bifidobacterium bifidum), 비피도박테리움 락티스(Bifidobacterium lactis), 비피도박테리엄 롱검(Bif. Longum) 중에서 선택된 1종 이상이 적용될 수 있다.It is appropriate to include 0.1 to 10 parts by weight of the lactic acid bacteria based on 100 parts by weight of the mixture, but the species is not particularly limited and examples include Lactiplantibacillus plantarum and Lactobacillus acidophilus. ), Lacticaseibacillus casei, Lactobacillus bulgaricus, Bifidobacterium bifidum, Bifidobacterium lactis, Bifidobacterium longum ( Bif. Longum) may be applied.
그 다음으로 피톤치드를 포함하는 사료조성물에 상기 유산균 발효액을 혼합하여 펠렛조성물을 제조하는 단계(S30)를 갖는다. Next, there is a step (S30) of preparing a pellet composition by mixing the lactic acid bacteria fermentation broth with a feed composition containing phytoncide.
본 단계(S30)에서 상기 사료조성물에는 옥수수분, 곡류부산물, 피톤치드, 여주추출물, 감귤박 건조분말을 포함하는 것을 특징으로 한다. 바람직하게는 옥수수분 100중량부에 대해, 곡류부산물 20 내지 50중량부, 피톤치드 5 내지 10중량부, 여주추출물 1 내지 3중량부, 감귤박 건조분말 0.5 내지 1중량부로 배합됨이 타당하다. In this step (S30), the feed composition includes corn meal, grain by-products, phytoncide, bitter melon extract, and citrus peel dried powder. Preferably, for 100 parts by weight of corn flour, 20 to 50 parts by weight of grain by-products, 5 to 10 parts by weight of phytoncide, 1 to 3 parts by weight of bitter melon extract, and 0.5 to 1 part by weight of dried citrus peel powder are appropriate.
상기 곡류부산물은 단백피, 소맥피, 및 보리겨로 구성된 군으로부터 선택된 하나 이상을 적용할 수 있다. The grain by-product may be one or more selected from the group consisting of protein hulls, wheat hulls, and barley bran.
상기 피톤치드(phytoncide)는 스트레스 완화, 항균, 소취 및 유해물질 중화, 진정작용 및 쾌적효과, 알레르기 및 피부질환 개선 등의 효과를 발휘하는 것으로 알려져 있다. The phytoncide is known to exert effects such as stress relief, antibacterial, deodorizing and neutralizing harmful substances, soothing and pleasant effects, and improving allergies and skin diseases.
이러한 피톤치드는 다양한 공지의 방법을 이용하여 첨가될 수 있는데, 예로 피톤치드를 함유한 율마 추출분말로서 첨가될 수 있다. 피톤치드를 함유한 율마 추출분말은 율마의 잎과 줄기를 채취하고 건조한 후에 파쇄하고 에탄올에 의해 추출 후 추출박과 분리되도록 여과하여 제조되도록 할 수 있다. These phytoncides can be added using various known methods, for example, they can be added as Yulma extract powder containing phytoncide. Yulma extract powder containing phytoncide can be manufactured by collecting the leaves and stems of Yulma, drying them, crushing them, extracting them with ethanol, and then filtering them to separate them from the extracted extract.
상기 여주추출물은 고온압축에 의해 제조되는 펠렛사료에 비타민 C가 안정적으로 존재토록 하기 위한 것이다. 비타민 C는 소의 스트레스를 줄여주는 효능 등이 발현되는 것인데 이러한 비타민 C는 일반적으로 고온에서 파괴가 되는 바, 특히 본 발명의 사료조성물을 고온에서 압축성형에 의해 펠렛화가 이루어지도록 하는데, 이 과정에서 비타민 C의 파괴를 수반할 수 있게 되는 것이다. The bitter melon extract is used to ensure that vitamin C is stably present in pellet feed produced by high-temperature compression. Vitamin C has the effect of reducing stress in cattle, and this vitamin C is generally destroyed at high temperatures. In particular, the feed composition of the present invention is pelletized by compression molding at high temperature, and in this process, vitamin C is destroyed at high temperatures. This could entail the destruction of C.
그런데 여주추출물에 함유된 비타민 C의 경우 고온하에서도 안정적으로 유지가 되는 효능이 있어 고온압축에 의해 제조되는 펠렛사료에 비타민 C가 안정적으로 존재토록 하는 것이다. However, vitamin C contained in bitter melon extract has the effect of remaining stable even at high temperatures, allowing vitamin C to exist stably in pellet feed manufactured by high-temperature compression.
여주는 아시아에서 널리 재배되는 박과의 다년생 덩굴식물로서, 이러한 여주에는 비타민 C가 100그램 중 120밀리그램이나 들어 있는데, 이는 딸기(80mg/100g), 레몬(90mg/100g), 양배추(40mg/100g)보다 월등히 많은 양이다. 특히 여주가 함유한 비타민 C의 경우 열을 가하여도 파괴되지 않는 특성이 있다. Bitter gourd is a perennial vine of the cucurbit family widely cultivated in Asia. Bitter melon contains as much as 120 milligrams of vitamin C per 100 grams, compared to strawberries (80 mg/100 g), lemons (90 mg/100 g), and cabbage (40 mg/100 g). ) is a much larger amount than In particular, the vitamin C contained in bitter melon has the property of not being destroyed even when heated.
이러한 여주추출물은 원료인 여주를 40메쉬 이하로 잘게 분쇄하여 수분함량 10중량%이하로 건조하고 분쇄 여주량의 5배에서 10배의 95중량% 주정알콜(-20℃에 보관된 것, 이하 ‘에탄올’로 표기)을 첨가하여 4℃에서 24시간 이상 충분히 1차 추출한 뒤 원심분리하여 상등액인 에탄올 층은 버리고, 에탄올에 녹지 않은 침전물량에 5배에서 10배의 70중량% 에탄올(-20℃에 보관된 것)을 첨가하여 4℃에서 12시간 이상 2차 추출하여 나온 에탄올 층을 증발 건조하여 점성이 높은 추출물이 제조되도록 하는 것이다. This bitter melon extract is made by finely grinding the raw material bitter melon to 40 mesh or less, drying it to a moisture content of 10% by weight or less, and adding 5 to 10 times the amount of ground bitter melon to 95% by weight of distilled alcohol (stored at -20°C, hereinafter ' After sufficient primary extraction at 4°C for more than 24 hours, the ethanol layer, which is the supernatant, is discarded, and 70% by weight ethanol (-20°C) is added 5 to 10 times the amount of precipitate not dissolved in ethanol. (stored in) is added and the ethanol layer obtained from secondary extraction for more than 12 hours at 4°C is evaporated to dryness to produce a highly viscous extract.
또한 상기 여주추출물은 점성에 의해 점성제로서 기능도 발현되도록 하는 것이다. 즉 펠렛의 견고한 형상유지가 가능하도록 하는 것이다. In addition, the bitter melon extract functions as a viscous agent due to its viscosity. In other words, it is possible to maintain the solid shape of the pellet.
마지막으로 상기 펠렛조성물을 압축성형 하여 펠렛사료를 제조하는 단계(S40)를 갖는다. 펠렛 사료는 열처리에 의하여 펠렛 형태로 제조하는 것으로, 열처리에 의하여 특히 분해, 흡수가 용이하게 되므로, 사료의 소화흡수율이 향상된다.Finally, there is a step (S40) of producing pellet feed by compression molding the pellet composition. Pellet feed is manufactured in the form of pellets through heat treatment, and heat treatment makes it particularly easy to decompose and absorb, thereby improving the digestion and absorption rate of the feed.
이하에서는 실험예에 의해 본 발명의 바람직한 실시예를 설명한다. Hereinafter, preferred embodiments of the present invention will be described through experimental examples.
<실시예 ><Example>
실시예는 전술한 유산균 배지성분인 펩톤, 효모추출물, 포도당, 구연산, 소듐 아세테이트, 천일염, 곡물인 볶은 귀리 분말, 참솔 송이버섯 분말, 사카로마이세스 세레비지에 효모 분말을 잘 혼합한 다음 적량의 물에 녹이고 고압 멸균기로 121℃로 총량 3리터 기준으로 15분 동안 가열 멸균한다. 멸균된 액상의 배지액이 37℃로 식으면 유산균을 접종하고 72시간 배양하였다. In the example, the above-mentioned lactic acid bacteria medium components such as peptone, yeast extract, glucose, citric acid, sodium acetate, sea salt, roasted oat powder, Chamsol pine mushroom powder, and Saccharomyces cerevisiae yeast powder were mixed well and then added in an appropriate amount. Dissolve in water and heat sterilize in a high-pressure sterilizer at 121°C for 15 minutes based on a total volume of 3 liters. When the sterilized liquid medium cooled to 37°C, lactic acid bacteria were inoculated and cultured for 72 hours.
본 실시의 예에서 사용한 균주는 락티플란티바실러스 플란타륨(Lactiplantibacillus plantarum, 예전명칭 락토바실러스 플란타륨 ; Lactobacillus plantarum)을 1중량%로 접종하여 배양배지의 첨가성분 함량 변화와 배양 시간에 따른 균체수 증감, pH의 변화를 비교하였다. 본 발명의 실시예에서 고온 멸균기는 C-AC, 유산균 배양기는 C-SKI-3(창신 과학, 대한민국)을 사용하였다.The strain used in this example was inoculated with Lactiplantibacillus plantarum (formerly known as Lactobacillus plantarum) at 1% by weight, and the bacterial cells were inoculated according to the change in the content of added ingredients in the culture medium and the culture time. Increases and decreases in water and changes in pH were compared. In the examples of the present invention, the high-temperature sterilizer C-AC and the lactic acid bacteria incubator C-SKI-3 (Changshin Science, Republic of Korea) were used.
<실시예 1><Example 1>
유산균 배지 성분으로 물 100중량부에 펩톤 2중량부, 효모추출물 1중량부, 포도당 2중량부, 구연산 2중량부, 소듐 아세테이트 5중량부, 천일염 0.85중량부, 볶은 귀리분말 5중량부, 참솔 송이버섯 분말 1중량부, 사카로마이세스 세레비지에 효모분말 2중량부를 첨가하고 전체 배지액의 pH를 6.2로 교정하고 고압 멸균기에서 15파운드 기압, 121℃로 가열 살균하여 37℃로 식힌 다음 유산균을 접종하여 37℃로 72시간 발효 배양하였다. . Lactobacillus medium ingredients include 100 parts by weight of water, 2 parts by weight of peptone, 1 part by weight of yeast extract, 2 parts by weight of glucose, 2 parts by weight of citric acid, 5 parts by weight of sodium acetate, 0.85 parts by weight of sun-dried salt, 5 parts by weight of roasted oat powder, and pine needles. Add 1 part by weight of mushroom powder and 2 parts by weight of yeast powder to Saccharomyces cerevisiae, correct the pH of the entire medium to 6.2, sterilize by heating at 121°C at 15 pounds of air pressure in an autoclave, cool to 37°C, and add lactic acid bacteria. It was inoculated and cultured for fermentation at 37°C for 72 hours. .
<실시예 2><Example 2>
유산균 배지 성분으로 물 100중량부에 펩톤 2중량부, 효모추출물 1중량부, 포도당 2중량부, 구연산 2중량부, 소듐 아세테이트 5중량부, 천일염 0.85중량부, 볶은 귀리분말 5중량부, 참솔 송이버섯 분말 1중량부, 사카로마이세스 세레비지에 효모분말 2중량부, 감귤 건조분말 1중량부를 첨가하고 전체 배지액의 pH를 6.2로 교정하고 고압 멸균기에서 15파운드 기압, 121℃로 가열 살균하여 37℃로 식힌 다음 유산균을 접종하여 37℃로 72시간 발효 배양하였다. Lactobacillus medium ingredients include 100 parts by weight of water, 2 parts by weight of peptone, 1 part by weight of yeast extract, 2 parts by weight of glucose, 2 parts by weight of citric acid, 5 parts by weight of sodium acetate, 0.85 parts by weight of sun-dried salt, 5 parts by weight of roasted oat powder, and pine cones. Add 1 part by weight of mushroom powder, 2 parts by weight of Saccharomyces cerevisiae yeast powder, and 1 part by weight of dried citrus powder, correct the pH of the entire medium to 6.2, and sterilize by heating at 121°C at 15 pounds of air pressure in a high-pressure sterilizer. After cooling to 37°C, lactic acid bacteria were inoculated and fermented and cultured at 37°C for 72 hours.
<실시예 3><Example 3>
유산균 배지 성분으로 물 100중량부에 펩톤 2중량부, 효모추출물 1중량부, 포도당 2중량부, 구연산 2중량부, 소듐 아세테이트 5중량부, 천일염 0.85중량부, 볶은 귀리분말 5중량부, 참솔 송이버섯 분말 1중량부, 사카로마이세스 세레비지에 효모분말 2중량부, 감귤 건조분말 1중량부, 보리수열매 건조분말 0.5중량부를 첨가하고 전체 배지액의 pH를 6.2로 교정하고 고압 멸균기에서 15파운드 기압, 121℃로 가열 살균하여 37℃로 식힌 다음 유산균을 접종하여 37℃로 72시간 발효 배양하였다. Lactobacillus medium ingredients include 100 parts by weight of water, 2 parts by weight of peptone, 1 part by weight of yeast extract, 2 parts by weight of glucose, 2 parts by weight of citric acid, 5 parts by weight of sodium acetate, 0.85 parts by weight of sun-dried salt, 5 parts by weight of roasted oat powder, and pine needles. Add 1 part by weight of mushroom powder, 2 parts by weight of Saccharomyces cerevisiae yeast powder, 1 part by weight of dried citrus powder, and 0.5 part by weight of dried barley fruit powder, correct the pH of the entire medium to 6.2, and sterilize 15 pounds in a high-pressure sterilizer. It was sterilized by heating at 121°C at atmospheric pressure, cooled to 37°C, then inoculated with lactic acid bacteria and fermented and cultured at 37°C for 72 hours.
<비교예><Comparative example>
유산균 배지 성분으로 물 100중량부에 펩톤 2중량부, 효모추출물 1중량부, 포도당 2중량부, 구연산 2중량부, 소듐 아세테이트 5중량부, 천일염 0.85중량부, 사카로마이세스 세레비지에 효모분말 2중량부를 첨가하고 전체 배지액의 pH를 6.2로 교정하고 고압 멸균기에서 15파운드 기압, 121℃로 가열 살균하여 37℃로 식힌 다음 유산균을 접종하여 37℃로 72시간 발효 배양하였다. Lactobacillus medium ingredients include 2 parts by weight of peptone, 1 part by weight of yeast extract, 2 parts by weight of glucose, 2 parts by weight of citric acid, 5 parts by weight of sodium acetate, 0.85 parts by weight of sea salt, and Saccharomyces cerevisiae yeast powder per 100 parts by weight of water. 2 parts by weight were added, the pH of the entire medium was corrected to 6.2, sterilized by heating to 121°C at 15 pounds of air pressure in a high-pressure sterilizer, cooled to 37°C, and then inoculated with lactic acid bacteria and fermented and cultured at 37°C for 72 hours.
(24h, 48h, 72h)pH change over time
(24h, 48h, 72h)
(cfu/ml) Hourly bacterial count measurement
(cfu/ml)
상기 표 1에서 보는 바와 같이 비교예에 비해 실시예들이 프리바이오틱스인 베타글루칸 섬유를 함유하는 볶은 귀리분말 및 참솔 송이버섯 분말이 더 첨가됨에 따라 균체수 증가에 영향을 미치고 있는 것을 알 수 있으며, 실시예 1보다 실시예 2 및 실시예 3의 경우가 감귤 건조분말에 의해 균체수 증가에 영향을 미치고 있는 것을 알 수 있다. As shown in Table 1, it can be seen that compared to the comparative example, the examples have an effect on the increase in the number of bacterial cells as more roasted oat powder and Chamsol pine mushroom powder containing prebiotic beta-glucan fiber are added, It can be seen that Example 2 and Example 3 have an effect on increasing the number of bacterial cells due to the dried citrus powder more than Example 1.
Claims (4)
상기 혼합액에 유산균을 접종하여 유산균 발효액을 제조하는 단계(S20);
피톤치드를 포함하는 사료조성물에 상기 유산균 발효액을 혼합하여 펠렛조성물을 제조하는 단계(S30);
상기 펠렛조성물을 압축성형 하여 펠렛사료를 제조하는 단계(S40);
를 포함하는 것을 특징으로 하는 가축 펠렛사료 제조방법.
Preparing a mixed solution containing roasted oat powder, Chamsol pine mushroom powder, and Saccharomyces cerevisiae yeast (S10);
Preparing a lactic acid bacteria fermentation broth by inoculating the mixed solution with lactic acid bacteria (S20);
Preparing a pellet composition by mixing the lactic acid bacteria fermentation broth with a feed composition containing phytoncide (S30);
Manufacturing pellet feed by compression molding the pellet composition (S40);
A method for manufacturing livestock pellet feed, comprising:
상기 사료조성물은, 옥수수분, 곡류부산물, 피톤치드, 여주추출물, 감귤박 건조분말을 포함하는 것을 특징으로 하는 가축 펠렛사료 제조방법.
According to clause 1,
The feed composition is a livestock pellet feed manufacturing method, characterized in that it contains corn meal, grain by-products, phytoncide, bitter melon extract, and citrus peel dry powder.
S10단계에는 감귤박 건조분말이 더 포함되는 것을 특징으로 하는 가축 펠렛사료 제조방법.
According to clause 1,
A method for manufacturing livestock pellet feed, characterized in that citrus peel dry powder is further included in step S10.
S10단계에는 보리수열매 건조분말이 더 포함되는 것을 특징으로 하는 가축 펠렛사료 제조방법.
According to clause 1,
A method for producing livestock pellet feed, characterized in that step S10 further includes dried barley fruit powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020220058033A KR20230158358A (en) | 2022-05-11 | 2022-05-11 | Manufacturing Method for Livestock Pellet Feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020220058033A KR20230158358A (en) | 2022-05-11 | 2022-05-11 | Manufacturing Method for Livestock Pellet Feed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20230158358A true KR20230158358A (en) | 2023-11-20 |
Family
ID=88974201
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020220058033A KR20230158358A (en) | 2022-05-11 | 2022-05-11 | Manufacturing Method for Livestock Pellet Feed |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR20230158358A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040069487A (en) | 2003-01-29 | 2004-08-06 | 정연화 | Feed including garlic for poultry |
-
2022
- 2022-05-11 KR KR1020220058033A patent/KR20230158358A/en unknown
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040069487A (en) | 2003-01-29 | 2004-08-06 | 정연화 | Feed including garlic for poultry |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108813098B (en) | Microbial fermentation composite antibiotic-free feed containing natural antibiotic components | |
| KR101904622B1 (en) | The functionality feed additive and functionality feed manufacturing method | |
| CN110353098A (en) | A kind of corn stover fermented feed and preparation method thereof | |
| KR101386879B1 (en) | Method for producing a fermentation food using mixed lactic acid bacteria and a fermentation food | |
| KR102106441B1 (en) | Feed composition for domestic animals comprising cricket powders and manufacturing method thereof | |
| CN110810625A (en) | Waste mushroom stick biological fermentation feed and preparation method thereof | |
| KR102181246B1 (en) | Feed composite for korean cattle | |
| KR20230037529A (en) | Preparation method of ingredients powder fermented by lactic acid bacteria | |
| CN106858038B (en) | Composite probiotic fermented feed and manufacturing process thereof | |
| JP7042834B2 (en) | How to make fermented guar meal | |
| KR101955775B1 (en) | Functional fermented food additive and functionality health food manufacturing method | |
| KR102210145B1 (en) | Method for preparing calcium-fortified heat-resistant feed additive and feed additive prepared thereby | |
| CN109287853B (en) | Corn straw-soybean meal mixed fermentation material, and preparation method and application thereof | |
| KR102017341B1 (en) | Feed composition for pork pig | |
| CN106721068A (en) | Composition and feed for adjusting animal intestinal tract microbial environment | |
| KR101501094B1 (en) | Manufacturing method of fermented feed with residual product | |
| KR102473715B1 (en) | Method for Manufacturing Subsidiary Feeder for Livestock Containing Coffee | |
| KR102370009B1 (en) | Manufacturing method for feed composition for pet dogs and feed composition for pet dogs manufactured using the same | |
| CN109699860A (en) | A kind of ferment drink and preparation method thereof | |
| KR20230158358A (en) | Manufacturing Method for Livestock Pellet Feed | |
| KR102159821B1 (en) | Fermented feed composition for ruminant and manufacturing method thereof | |
| KR101691364B1 (en) | Bee subsidiary feeder composition comprising rice bran and manufacturing method thereof | |
| CN107183484A (en) | It is a kind of for the composition of feedstuff mildew, preparation method and its application method | |
| CN106937711A (en) | A kind of feed for preventing and treating hydropsy for baby pigs and preparation method thereof | |
| KR20120103940A (en) | Manufacturing method of fermented fig materials |