KR20230092481A - A spray-type cultivation method of korean ginseng with enhanced saponin content - Google Patents

Abstract

The present invention relates to a spray cultivation method of ginseng sprouts with increased saponin content, and more specifically,
A primary culture step of irradiating ginseng seedlings with LED light for 7 to 9 hours a day for 3 to 7 days and spraying a nutrient solution; A secondary culture step of irradiating the primary cultured seedlings with LED light for 9 to 11 hours a day for 7 to 14 days and spraying a nutrient solution; And a tertiary culture step of irradiating the secondary cultured seedlings with LED light for 11 to 13 hours a day for 7 to 21 days and enhancing the saponin content of spraying the nutrient solution; wherein the LED light irradiation is red: blue : Yellow = 2: 1: Disclosure of a spray cultivation method of sprout ginseng with increased saponin content, characterized in that the light source composed of 2: 1: 1 is irradiated with a photosynthetic amount of 50 to 100 mmol m ^-2 s ^-1 do.

Description

A spray-type cultivation method of korean ginseng with enhanced saponin content}

The present invention relates to a spray cultivation method of ginseng sprouts with increased saponin content.

Saponine is a very valuable compound in the pharmaceutical, cosmetic, agricultural, and food markets, and is contained in soybeans, green onions, deodeok, bellflower, water parsley, garlic, onion, ganoderma lucidum, kudzu, hyssop, and ginseng.

Saponin is a type of glycoside contained in the roots, leaves, and stems of various plants. It has a bitter and astringent taste and is produced as a substance for the defense system of plants. The amount of saponin produced is greatly influenced by changes in the external environment.

More specifically, in order to increase the content of saponin, it is to produce an excessive amount of saponin by giving various stress factors to plants. That is, as an example of a case where the saponin content of a plant is high, a plant responds to external factors including an attack by herbivores, infection with plant pathogens, and biological stimuli related to rhizobacteria and their symbiotic interactions with plants. This change will be huge.

In Korea, ginseng is the most well-known plant that contains a large amount of saponin. In relation to this, ginseng saponin has a different chemical structure from other plant saponins, and to distinguish it, it is called ginsenoside, a compound word of ginseng and glycoside. Generally, Rg1 and Re account for the majority of the content of ginsenosides is extracted

In relation to this, Rg1 has an effect of enhancing immune function, improving memory learning function, and anti-fatigue action, and Re is known to have liver protective action, bone marrow cell synthesis promoting action, and vasodilation effect, so it is frequently used as a variety of drugs.

However, in the case of the above ginseng, there is a problem that it takes a long time to grow, since most of the 4-year-old roots are 5-6 years old. In addition, when using ginseng grown for a long time, only the root part is used, so the amount is not large compared to the yield.

According to recent research results, it has been reported that not only the leaves of ginseng contain saponin compounds that are not present in the root, but also that the leaves and stems contain a greater amount of saponin in the case of 4-year-old roots. However, domestic ginseng is vulnerable to pests and uses a large amount of pesticides, so the risk of residual pesticides is quite high to consume the leaves. In addition, in the case of ginseng leaves, since they are rigid, it is difficult to ingest, so transformation into processed foods using them is required.

In order to improve the above problems, many studies have been conducted on growing ginseng sprouts that can be consumed from leaves to roots by cultivating ginseng seedlings in a short period of time using culture soil and hydroponic cultivation.

In this regard, according to Korean Registered Patent No. 10-1836799 “Cultivation Method of Ginseng Sprouts,” ginseng sprouts cultivation method in which ginseng seedlings with partially exposed brain heads are transplanted into mixed soil mixed with topsoil composition, peatmus and masato. is describing.

However, in the case of the above method, there is a problem in that the cost of constructing the initial facility is large as well as the space limitation to use the culture soil is large. In addition, since ginseng sprouts are intended for quick harvest within one month, there is a problem in that new culture soil must be prepared to grow new ginseng sprouts.

According to Korean Patent Publication No. 10-2021-0085536 “Sprout Ginseng Cultivation System and Method”, a desalination apparatus is used and sterilization of circulating water using plasma describes a technique for removing germs from ginseng seedlings. .

However, in the case of the above method, there is a problem in that ginseng sprouts are not uniformly cultivated because they have a structure in which water is circulated, and ginseng sprouts initially disposed absorb all nutrients. In addition, even if the water is continuously sterilized with plasma, if the sterilization is not 100% complete, ginseng sprouts are all infected with pathogens according to the flow of the circulating waterway, and the problem of propagation such as root rot still remains.

According to Korean Patent Publication No. 10-2021-0098925 “Method for Cultivating Ginseng Sprouts or Increasing Saponin Content Using LED”, it describes a technique of irradiating both near-infrared light and red, green, and blue LEDs as a light source. .

However, in the case of the above method, only the function for promoting photosynthesis of simple sprout ginseng is described. In addition, in the case of LED light having a function of promoting photosynthesis, depending on the combination, there is a problem that can cause fatigue to the eyes of growers as it emits a purple light color.

Republic of Korea Patent No. 10-1836799 Republic of Korea Patent Publication No. 10-2021-0085536 Republic of Korea Patent Publication No. 10-2021-0098925

Therefore, an object of the present invention is to solve the above problems, and more specifically, ginseng sprouts having a high content of saponin in a short period of time by adjusting the LED light color, LED light irradiation time, nutrient solution composition, and spraying time for each schedule. It is a technical challenge to provide a spray cultivation method for sprout ginseng that can not only grow but also has no risk of residual pesticides.

In addition, as a technical solution, providing ginseng sprouts with a higher growth rate than general cultivation methods by going through the 1st and 2nd cultivation process in the cultivation stage as well as solving the phenomenon of rotting or weakening the roots during the cultivation process of ginseng sprouts. do.

In order to solve the above technical problem, the present invention,

A primary culture step of irradiating ginseng seedlings with LED light for 7 to 9 hours a day for 3 to 7 days and spraying a nutrient solution; A secondary culture step of irradiating the primary cultured seedlings with LED light for 9 to 11 hours a day for 7 to 14 days and spraying a nutrient solution; And a tertiary culture step of irradiating the secondary cultured seedlings with LED light for 11 to 13 hours a day for 7 to 21 days and enhancing the saponin content of spraying the nutrient solution; wherein the LED light irradiation is red: blue : Yellow = 2: 1: Provides a spray cultivation method of sprout ginseng with increased saponin content, characterized in that the light source composed of 2: 1: 1 is irradiated with a photosynthetic amount of 50 to 100 mmol m ^-2 s ^-1 .

Preferably, the nutrient solution spray is characterized in that the mineral solution at a concentration of 8,000 to 10,000 ppm is sprayed on the roots of the ginseng seedlings, and the spray cycle is adjusted so that the relative humidity is maintained at 65 to 75%.

Preferably, the mineral solution is vermiculite, zeolite, dietary sulfur, peat moss, potassium nitrate, calcium nitrate, ferric sodium EDTA, ammonium phosphate monobasic, magnesium sulfate, boric acid, manganese sulfate, zinc sulfate, copper sulfate, germanium, molybdenum, nickel, It is characterized in that it includes at least one of barium, selenium, lithium, zinc, rubidium, vanadium, sodium, biotite, muscovite, calcium carbonate and sodium molybdate.

Preferably, the ginseng seedlings are 1-year to 3-year-old ginseng seedlings.

Preferably, the primary culturing step is to break the dormant state of the ginseng seedlings by raising the storage temperature of the seedlings stored frozen at -3 to -5 ° C to 3 to 5 ° C for 5 to 7 days; And it is characterized in that it consists of a step of irradiating LED light for 7 to 9 hours a day for 3 to 7 days and spraying a nutrient solution on the seedlings that have undergone the above steps.

The present invention has the effect of mass-cultivating ginseng sprouts having a high content of saponin in a short period of time by providing a spray cultivation method of ginseng sprouts for each LED light color, LED light irradiation time, nutrient solution composition, and spraying time. there is. In particular, among the saponin components, the Re component and the Rd component have a high effect. In addition, it has the effect of extracting F1 and F2, which are rare saponin components, from the leaves.

In addition, the present invention not only has the effect of preventing root rot of ginseng sprouts and the phenomenon of ginseng sprouts shrinking, but also has the effect of cultivating ginseng sprouts with a higher growth rate than general cultivation methods through primary and secondary cultivation there is

In addition, the present invention has the effect that residual pesticides do not exist.

1 is a schematic diagram of a spray cultivation method of ginseng sprouts according to an embodiment of the present invention.
Figure 2 is a table showing a color comparison picture according to the LED light ratio of the present invention.
Figure 3 shows test conditions for confirming the ginsenoside content by site through HPLC analysis according to Test Example 1 of the present invention.
4 is an HPLC chromatogram showing the concentration of ginsenoside in leaves according to Test Example 1 of the present invention.
5 is an HPLC chromatogram showing the ginsenoside concentration of the root according to Test Example 1 of the present invention.
6 is an HPLC chromatogram showing the concentration of ginsenoside in the stem according to Test Example 1 of the present invention.
7 is a test report showing the pesticide residue results for 320 species according to Example 2 of the present invention.
8 is a test report showing the pesticide residue results for 320 species according to Comparative Example 2 of the present invention.
9 is a test report showing the pesticide residue results for 473 species according to Example 2 of the present invention.

Hereinafter, the present invention will be described in detail with reference to FIG. 1 .

The present invention is a primary culture step (S100) of irradiating ginseng seedlings with LED light for 7 to 9 hours a day for 3 to 7 days and spraying a nutrient solution; A secondary culture step (S200) of irradiating the primary cultured seedlings with LED light for 9 to 11 hours a day for 7 to 14 days and spraying a nutrient solution; and a tertiary culturing step (S300) of irradiating the secondary cultured seedlings with LED light for 11 to 13 hours a day for 7 to 21 days and enhancing the saponin content by spraying the nutrient solution.

Hereinafter, the detailed configuration will be described prior to the description of each culture step.

First, LED (Light Emitting Diode) light commonly included in the process of the present invention uses a light emitting diode made of a material containing gallium, phosphorus, and arsenic.

Compared to other light sources, LED light has a longer lifespan, higher efficiency, and a higher photosynthetic effective photon flux density (PPDF) compared to power consumption.

The LED light source is each monochromatic light such as red, green, blue, and white, and has unique light wavelength characteristics, so when used as an individual light source or combined as a mixed light, it affects the growth of seedlings and the enhancement of saponin.

In relation to this, the characteristics of each colored light will be briefly described.

First, red light has properties effective for promoting photosynthesis and controlling flowering, and super-red light has the effect of increasing the sugar content of fruit trees, increasing vitamins and increasing functional substances. Therefore, in the case of sprout ginseng belonging to special crops, there is a significant effect on the increase of saponin substances. Preferably, it is most preferable to use red light having a wavelength of 645 to 700 nm.

Blue light has an effect on growth and has properties effective for photosynthesis, and it is most preferable to use blue light having a wavelength of 430 to 480 nm.

Green light is a safety light and has an auxiliary role in photosynthesis. Preferably, green light having a wavelength of 490 to 530 nm is used.

Yellow light has properties effective for controlling insect pests, and preferably, yellow light having a wavelength of 550 to 580 nm is used.

In the present invention, among the light of the effective color, mixed light in which red, blue, and yellow are combined is used, and it is preferably included in a ratio of 2:1:1. This increases the photosynthetic promoting effect of ginseng sprouts by the combination of red light and blue light, thereby promoting growth and increasing the saponin content.

In addition, it has the function of suppressing insect pests, which is a characteristic of yellow light, so it has the effect of reducing the effect of ginseng sprouts being infected with pathogens, as well as reducing fatigue to the eyes of growers by showing a light purple light due to the combination of light. There is a noticeable difference as shown in Figure 2.

On the other hand, in the case of the light source, the smaller the irradiation amount, the larger the size of the ginseng seedlings. However, as the ginseng seedlings become softer, the quality of the product decreases. Therefore, the light source is preferably irradiated with a photosynthetic amount of 50 to 100 mmol·m ^-2 ·s ^-1 .

Next, the nutrient solution forms a solid medium or root system without using soil, dilutes the nutrients required for the growth of ginseng sprouts to an appropriate concentration according to the absorption rate, and artificially supplies the culture medium to promote photosynthesis of ginseng sprouts, It has the effect of increasing the amount of enzymes. In addition, disease caused by mineral deficiency It plays a role in preventing, enhancing the content of saponin and removing fine residual pesticides.

In this regard, in general, essential elements required for crop growth include C (carbon), O (oxygen), H (hydrogen), N (nitrogen), P (phosphorus), K (potassium), Ca (calcium), Mg ( Magnesium), S (sulfur), Fe (iron), Mn (manganese), Cu (copper), Zn (zinc), B (boron), Mo (molybdenum), and Cl (chlorine) as essential elements. Of these, large amounts of elements (types: C, O, H, N, P, K, Ca, Mg, S) and trace elements that need to be supplied in small amounts (types: Fe, Mn, Cu, Zn, B, Mo , Cl ) .

Among the essential elements, C, O, and H are supplied from carbon dioxide and water in the atmosphere, so they do not need to be supplied as nutrient solution. Cl also needs to be supplied as nutrient solution, except for special cases, as crop demand is low and it is often contained in water to some extent. there is no Therefore, it can be said that 12 elements are essential elements in the nutrient solution.

On the other hand, the nutrient solution should contain inorganic nutrients necessary for sprout ginseng and not contain harmful ingredients to sprout ginseng. In addition, it is characterized by being easily absorbed by the root of sprout ginseng, that is, it must be dissolved in water in an ionic state.

In relation to this, it is preferable that the composition ratio (%) of the nutrient solution satisfies the following relational expressions 1 and 2,

[Relationship 1]

No ₃ -N (nitrate nitrogen) = K (potassium) + Ca (calcium)

[Relationship 2]

No ₃ -N (nitrate nitrogen) : P (phosphorus) : Ca (calcium) = 3~4 : 1 : 1

In the case of trace element content, Fe (iron) = 2-5 mg/ℓ, Mn (manganese) = 0.5 mg/ℓ, Cu (copper) = 0.05 mg/ℓ, Zn (zinc) = 0.05 mg/ℓ, B (Boron) = 0.5 mg/L, Mo (molybdenum) = 0.02 mg/L is preferably included.

In this regard, the nutrient solution used in the present invention is sprayed with a mineral solution at a concentration of 8,000 to 10,000 ppm to the roots of seedlings, but it is preferable to adjust the spraying cycle so that the relative humidity is maintained at 65 to 75%.

At this time, when the relative humidity is less than 65%, it is preferable to spray the nutrient solution for 1 to 2 minutes at an interval of 1 hour to adjust the relative humidity, and when the relative humidity exceeds 75%, 1 hour 30 to 2 hours It is desirable to spray the nutrient solution for 1 to 2 minutes periodically to control the humidity.

In relation to it, it is necessary to control the temperature of the cultivation environment together with humidity, preferably maintaining 18 to 22 ° C, and more preferably cultivating in a 21 ° C environment.

This is because the dew point may vary depending on humidity and temperature, and preferably, the temperature difference between the temperature and the dew point is maintained at 5 or more.

This is because when the dew point is less than 5, fungal bacteria may occur on the roots of ginseng sprouts, and the roots may become brittle.

On the other hand, it is preferable to use the nutrient solution having a pH in the range of 5.5 to 6.5. In relation to this, when the pH range of the nutrient solution is 6.5 or more, the solubility of trace elements such as Fe (iron), Mn (manganese), and B (boron) is lowered, thereby inhibiting absorption, and even in the case of P (phosphorus), Ca (calcium calcium) under alkalinity. ) to become a poorly soluble salt, thereby limiting the absorption and use of P (phosphorus) and Ca (calcium). On the other hand, when the pH is less than 5.5, the solubility of P (phosphorus), Ca (calcium), Mg (magnesium), etc. is lowered and absorption is lowered, which can affect growth.

Meanwhile, the mineral solution preferably includes clay-type minerals such as vermiculite, zeolite, peat moss, muscovite, biotite, and calcium carbonate in the form of an aqueous solution in order to remove residual pesticides from seedlings.

Therefore, preferably, the mineral solution is vermiculite, zeolite, dietary sulfur, peat moss, potassium nitrate, calcium nitrate, ferric sodium EDTA, ammonium phosphate monobasic, magnesium sulfate, boric acid, manganese sulfate, zinc sulfate, copper sulfate, germanium, molybdenum, nickel , barium, selenium, lithium, zinc, rubidium, vanadium, sodium, biotite, muscovite, and at least one of calcium carbonate and sodium molybdate.

Hereinafter, each culture step will be described.

First, a primary culturing step (S100) of irradiating LED light to seedlings and spraying nutrient solution; As a step, ginseng seedlings are irradiated with LED light for 7 to 9 hours a day for 3 to 7 days and sprayed with nutrient solution to cultivate ginseng seedlings. .

In the primary culture step, as a step for growing the size of ginseng seedlings, the absorption rate of nutrient solution can be increased by increasing the number of fine roots of ginseng seedlings, and residual pesticides can be removed through the nutrient solution.

Therefore, in the primary culture step for this purpose, it is preferable to spray the nutrient solution on the roots or leaves of the ginseng seedlings while illuminating them with the above-described LED light for 7 to 9 hours a day for 3 to 7 days.

More preferably, in order to increase the absorption rate of the nutrient solution, it is more preferable to spray the roots of ginseng seedlings.

On the other hand, it is preferable to use 1- to 3-year-old baejae ginseng grown from ginseng seeds for 1 to 3 years or commercially available 1 to 3-year-old ginseng seedlings.

This is because ginseng seedlings older than three years have more saponin content in the roots, so the saponin extraction content may be rather low.

When using ginseng seedlings sold on the market, it is preferable to use ginseng seeds weighing about 1g per piece, but the brain heads are clearly distinguished, the seedlings are not damaged by wounds and nematodes, and there is no frost damage or drying damage during storage.

In addition, in the case of cultivated ginseng seedlings, they can be collected twice a year, from September to November and from March to May. If necessary, ginseng seedlings are dormant by freezing in an environment of -3 to -5 ° C for 1 to 2 months. condition can be stored. At this time, it is preferable to use the frozen ginseng seedlings within 90 days. Of course, it can be used even if it exceeds 90 days. because it loses

At this time, in the case of using the frozen-preserved ginseng seedlings, a step of breaking the dormant state of the ginseng seedlings by raising the temperature of the ginseng seedlings to 3 to 5 ° C. for 5 to 7 days may be further included before the primary culturing step.

Specifically, it is preferable to complete the process of breaking dormancy by raising the temperature of the frozen seedlings by 0.5 to 2 ° C per day in a -3 to -5 ° C environment and raising the temperature to 3 to 5 ° C in 5 to 7 days. .

At this time, if the daily temperature is raised by more than 2℃, there is a possibility of fungal growth in the seedlings due to the sudden temperature change. This is because the growth of ginseng seedlings is delayed and the size is reduced as the productivity decreases and the period of irradiation with nutrient solution and LED light is delayed.

Next, a secondary culture step (S200) of irradiating LED light and spraying a nutrient solution on the primary cultured seedlings (S200); By irradiating light and spraying nutrient solution, ginseng seedlings photosynthesize most actively, so the growth of ginseng seedlings is most active, and the growth of leaves and stems is rapid at the time when nutrient solution absorption is significantly increased through the roots grown in the primary culture. This is the time when more than 90% of residual pesticides are removed.

In this regard, the nutrient solution spraying in the secondary culture step preferably satisfies the aforementioned spray conditions, and more preferably irradiates a larger amount of LED light than in the primary culture step.

Finally, a third culture step (S300) of irradiating the secondary cultured seedlings with LED light and spraying nutrient solution to increase the saponin content (S300); irradiating LED light for 11 to 13 hours a day for 7 to 21 days and nutrient solution This is the step of enhancing the saponin content of spraying.

Specifically, photosynthesis is performed with LED light for about 1/2 hour a day in the tertiary culturing step, which is a step in which saponin is excessively produced by giving stress factors to seedlings.

Preferably, it is preferable to culture for 7 to 21 days, and more preferably, culture for 14 days can maximize the freshness of seedlings.

On the other hand, the spray cultivation method of ginseng sprouts with increased saponin content is most preferably cultivated within a minimum of 3 weeks to a maximum of 6 weeks. This is because the content of saponin in the leaves and roots may decrease as it is concentrated in the fruit. On the other hand, in the case of cultivation for less than 3 weeks, the saponin content is also low in a state where sufficient growth is not achieved, resulting in poor marketability.

According to the above culturing steps, by gradually increasing the culturing period and the LED light irradiation time step by step, it is possible to maximize the growth of ginseng seedlings as well as to increase the saponin content.

In addition, by continuously supplying the nutrient solution for each cycle, it is possible to increase the growth rate of seedlings and also to remove residual pesticides.

Hereinafter, the present invention will be described in more detail through examples, but the present invention is not limited thereto.

<Examples 1 to 3>: Cultivation of ginseng sprouts

(1) Manufacture and storage of dormant seedlings

Ginseng seedlings grown for 1 to 3 years were collected and stored at -3 to -4 ° C for 30 days to prepare dormant ginseng seedlings, and the temperature was continuously maintained at the above temperature.

(2) Manufacture and storage of dormant seedlings

The dormant ginseng seedlings were raised by 1 ° C per day and thawed at room temperature for 7 days to break dormant ginseng seedlings.

(3) Growth and harvest of ginseng seedlings

The dormant broken ginseng seedlings were primary cultured by irradiating LED light for 8 hours for 5 days. Next, the primary cultured seedlings were irradiated with LED light for 10 hours for 10 days for secondary culture. Finally, the secondary cultured ginseng seedlings were irradiated with LED light for 12 hours for 15 days for tertiary cultivation to increase the sanin content, and then the cultivated ginseng sprouts were harvested.

At this time, the LED light used was composed of red: blue: yellow = 2: 1: 1 ratio, and the light source was irradiated with 80 mmol·m ^-2 ·s ^-1 .

In addition, the temperature inside the cultivation room was maintained at 19 ~ 21 ℃, and a mineral solution with a concentration of 10,000 ppm was sprayed on the roots of ginseng seedlings for 1 minute every hour, and the internal relative humidity was maintained at 65 ~ 66% during cultivation.

<Comparative Example 1>: Cultivation method of ginseng sprouts using 5-year-old ginseng seedlings

(1) Manufacture and storage of dormant seedlings

Five-year-old ginseng seedlings were collected and stored for 30 days at a temperature of -3 to -4 ° C to prepare dormant ginseng seedlings, and the temperature was continuously maintained at the above temperature.

(2) Manufacturing and storage of dormant ginseng seedlings/growth and harvesting of ginseng seedlings

The manufacturing and storage of dormant seedlings/growth and harvesting of seedlings were carried out in the same manner as in Examples 1 to 3.

<Comparative Example 2>: Cultivation method of sprout ginseng with different growth and harvesting methods of seedlings

(1) Manufacture and storage of dormant seedlings

Two-year-old ginseng seedlings were collected and stored at -3 to -4 ° C for 30 days to prepare dormant ginseng seedlings, and the temperature was continuously maintained at the above temperature.

(2) Manufacture and storage of dormant seedlings

The production and storage of dormant seedlings were carried out in the same manner as in Examples 1 to 3.

(3) Growth and harvest of ginseng seedlings

The dormant broken ginseng seedlings were irradiated with LED light for 10 hours for 30 days.

Other LED light conditions were performed in the same manner as in Examples 1 to 3.

<Test Example 1>: Test for confirming ginsenoside content by site through HPLC analysis

HPLC analysis was used to confirm the ginsenoside content of the leaves, roots, and stems of Examples 1 to 3 and Comparative Example 1.

The sample was prepared by dividing the ginseng sprout sample into leaves, stems, and roots, and the root was washed with distilled water and dried in a dry oven at 70 ° C for 12 hours to be powdered for analysis.

As an extraction method, 1 g of sample sample leaf was put in a conical tube and 50 ml of 50% Ethanol was added (50 times that of the original material), and 1 g of the root and stem was put in a 50 ml conical tube and 25 ml of 50% Ethanol was added. (25 times compared to original material)

Next, after taking the sample for analysis, drying at 50 ° C. for 12 hours, 1 ml of the sample was collected and centrifuged at 13,000 rpm for 5 minutes.

HPLC analysis was started by transferring 200 ul of the supernatant of each sample to an HPLC analysis sample bottle.

HPLC used gradient conditions as shown in FIG. 3, and 16 standard products (Rg1, Re, Rg2(S), Rb1, Rc, Rb2, Rb3, F1, Rd, Gyp17, C-Mc1, C-O, F2 , Rg3(S), C-K, Rh2(S)) were produced at 500 ppm, 125 ppm, 31.25 ppm, and 15.625 ppm, and a calibration curve was confirmed.

The ginsenoside concentrations of each standard are shown in Tables 1 to 3 below. In addition, the HPLC chromatogram for each site was separately marked in FIGS. 4 to 6.

Concentration of ginsenosides in leaves (based on dry matter): (unit, mg/g) ginsenoside Example 1
(1-year-old leaves) Example 2
(2-year-old leaves) Example 3
(3-year-old leaves) Comparative Example 1
(5-year-old leaves) Rg1 10.4 14.3 14.3 8.3 Re 39.3 47.3 41.0 34.7 Rg2(S) 6.1 7.0 7.4 6.9 Rb1 2.5 3.1 2.7 2.1 Rc 4.6 6.8 6.8 3.8 F1 3.7 3.9 4.2 2.4 Rb2 6.4 9.2 9.7 5.0 Rb3 1.0 1.1 1.3 0.7 Rd 21.9 31.9 27.6 17.3 Gyp17 0.8 0.4 0.5 0.2 C-Mc1 1.1 1.5 1.5 0.5 C-O 2.6 2.2 2.7 0.7 F2 6.2 6.6 6.6 3.4 TOTAL 106.5 135.6 126.4 86.1

Concentration of ginsenosides in roots (based on dry matter) (unit, mg/g) ginsenoside Example 1
(1-year-old root) Example 2
(two-year-old root) Example 3
(3-year-old root) Comparative Example 1
(5-year-old root) Rg1 3.1 4.0 3.8 6.6 Re 6.6 8.5 5.2 7.4 Rg2(S) 0.8 1.1 0.6 0.7 Rb1 5.9 7.3 4.7 9.4 Rc 2.5 3.9 2.2 3.0 F1 nd nd nd nd Rb2 2.3 4.1 2.1 2.6 Rb3 0.4 0.6 0.3 0.5 Rd 1.9 2.3 1.6 1.9 Gyp17 0.5 0.5 0.6 0.8 C-Mc1 0.1 0.2 0.2 0.1 C-O 0.5 0.3 0.3 0.2 F2 0.0 0.0 0.0 0.0 TOTAL 24.7 32.8 21.5 33.2

Concentration of ginsenosides in stems (based on dry matter) (unit, mg/g) ginsenoside Example 1
(1-year-old stem) Example 2
(two-year-old stem) Example 3
(3-year-old stem) Comparative Example 1
(5-year-old stem) Rg1 6.4 4.8 7.0 5.0 Re 6.9 5.9 6.1 7.0 Rg2(S) 1.2 1.1 1.0 1.0 Rb1 0.1 0.2 0.1 0.1 Rc 0.1 0.1 0.1 0.3 F1 0.8 0.7 1.0 0.6 Rb2 1.4 0.5 1.0 1.6 Rb3 nd nd nd nd Rd 0.9 0.8 1.1 0.9 Gyp17 0.1 0.0 0.1 0.0 C-Mc1 0.2 0.5 0.4 0.2 C-O 0.0 0.2 0.4 0.0 F2 1.6 0.9 1.3 1.3 TOTAL 19.8 15.7 19.6 18.1 nd: not detected. (not detected)

According to the analysis results, it was confirmed that ginsenoid Re and Rd components were excessively produced. Generally, ginsenosides extracted from ginseng are Rh2, Rh1, Rg3, Rg2, Rg1 Re. Rd. Rc Rb2. Rb1. It is common for Ro and Rf to be extracted, but in the case of ginseng sprouts cultivated in one embodiment, it was confirmed that rare ginsenosides F1 and F2 contents came out from the leaves.

For reference, the extracted F1 ginsenoside is characterized by having an immunity enhancing effect on wounds, and has a superior immune enhancing effect compared to Rg1. In addition, F2 ginsenoside is known to have anti-inflammatory and antipruritic effects.

On the other hand, according to the above results, it was confirmed that the highest ginsenoside content was found in 2-year-old ginseng sprouts.

In the case of the root, it was confirmed that the concentration was much lower, but it was confirmed that the ginsenoside content of 2-year-old and 5-year-old ginseng sprouts was similar.

In the case of the stem, the lowest ginsenoside content was confirmed compared to the leaves and roots. However, in the stem, Rg1 and Re, which are PPT (Protopanaxatriol) series, showed relatively high results. In addition, it was confirmed that the smallest amount of ginsenosides was extracted from 2-year-old ginseng sprouts, contrary to roots and leaves.

<Test Example 2>: Pesticide residue confirmation test 1

The ginseng sprouts cultivated by the method of Example 2 and Comparative Example 2 were inspected for residual pesticides for 320 pesticide residues by inquiring to the analysis center.

Specific test results are separately marked in FIGS. 7 and 8 .

According to the test results, in the case of Example 2 cultivated as a preferred embodiment, non-detection results were shown for 320 pesticide residues as shown in FIG. 7, and in Comparative Example 2 where the LED light cycle was irradiated under the same conditions for 30 days As shown in FIG. 8, the results of 0.071 mg / kg of pesticide Cadusafos and 0.060 mg / kg of Fludioxonil were shown. Although Comparative Example 2 showed a lower value than the reference value of pesticide residue, it was confirmed that a small amount was detected.

<Test Example 3>: Pesticide residue confirmation test 2

In addition, ginseng sprouts cultivated by the method of Example 2 were inspected for residual pesticides for 473 pesticide residues by inquiring to the analysis center.

Specific test results are separately marked in FIG. 9 .

According to the test results, in the case of Example 2 cultivated as a preferred embodiment, non-detection results were shown for 473 pesticide residues as shown in FIG. 9.

Since the specific parts of the present invention have been described in detail above, various modifications and variations will be possible to those skilled in the art without departing from the essential characteristics of the present invention. Therefore, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention, but are intended to explain, and the scope of the technical idea of the present invention is not limited by these embodiments. The protection scope of the present invention should be construed according to the claims, and all technical ideas within the scope equivalent thereto should be construed as being included in the scope of the present invention.

Claims (5)

A primary culture step of irradiating ginseng seedlings with LED light for 7 to 9 hours a day for 3 to 7 days and spraying a nutrient solution;
A secondary culture step of irradiating the primary cultured seedlings with LED light for 9 to 11 hours a day for 7 to 14 days and spraying a nutrient solution; and
A third culture step of irradiating the secondary cultured seedlings with LED light for 11 to 13 hours a day for 7 to 21 days and enhancing the saponin content by spraying the nutrient solution;
The irradiation of the LED light is red: blue: yellow = 2: 1: 1 ratio of 50 to 100 mmol m ^-2 s ^-1 photosynthetic amount of light source composed of, characterized in that, ginseng sprouts with increased saponin content spray cultivation method of According to claim 1
The nutrient solution spraying,
A spray cultivation method of ginseng sprouts with increased saponin content, characterized in that the mineral solution at a concentration of 8,000 to 10,000 ppm is sprayed on the roots of the ginseng seedlings, but the spray cycle is adjusted so that the relative humidity is maintained at 65 to 75%. According to claim 2
The mineral solution is vermiculite, zeolite, dietary sulfur, peat moss, potassium nitrate, calcium nitrate, ferric sodium EDTA, ammonium phosphate monobasic, magnesium sulfate, boric acid, manganese sulfate, zinc sulfate, copper sulfate, germanium, molybdenum, nickel, barium, selenium , Lithium, zinc, rubidium, vanadium, sodium, biotite, muscovite, and at least one of calcium carbonate and sodium molybdate Spray cultivation method of sprout ginseng with increased saponin content, characterized in that it contains According to claim 1
The ginseng seedling is a spray cultivation method of ginseng sprouts with increased saponin content, characterized in that the ginseng seedlings are 1 to 3 years old The method of claim 1, wherein the primary culture step,
Breaking the dormant state of the ginseng seedlings by raising the storage temperature of ginseng seedlings frozen at -3 to -5 ° C for 5 to 7 days to 3 to 5 ° C; and
Spray cultivation method of ginseng sprouts with increased saponin content, characterized in that it consists of the step of irradiating LED light for 7 to 9 hours a day for 3 to 7 days and spraying a nutrient solution on the seedlings that have undergone the above steps.

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