KR20230072619A - Cosmetic composition comprising turmeric saccharification fermented extract - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a saccharification and fermentation product of Curcuma longa, which can be useful for skin whitening and inflammation alleviation.

Description

Cosmetic composition comprising turmeric saccharification fermented extract {Cosmetic composition comprising turmeric saccharification fermented extract}

The present invention relates to a cosmetic composition comprising a saccharified fermentation product of turmeric, which may be useful for skin whitening and inflammation improvement.

Turmeric ( Curcuma longa L. ) is a rhizome of ginger and Zingiberaceae. It is a component of phenols and its glycosides, monoterpene glycosides, tannin, and other sucrose. actions have been reported. Traditionally, in oriental medicine, turmeric has been used as one of the medicinal materials that help blood circulation, and it does not only act on blood, but also has the effect of guiding well. It is known as a blood medicine because it even plays a role. Curcuminoid derivatives and essential oils have been reported as the main components of turmeric. Curcuminoid derivatives include diarylheptane, curcumin, related derivatives, and C9 chain of curcumin. Derivatives (curcumins I-III) have been reported, and as essential oils, sesquiterpene-related derivatives such as turmerone, curlone, and curcumene have been reported.

The present inventors have completed the present invention by finding that anti-inflammatory and whitening effects are remarkably improved in saccharification fermentation of turmeric.

Korean Patent Publication No. 10-2019-0001163

An object of the present invention is to provide a cosmetic composition for skin whitening comprising a saccharified fermentation product of turmeric.

Another object of the present invention is to provide a cosmetic composition for preventing or improving skin inflammation comprising a saccharified fermentation product of turmeric.

In order to achieve the above purpose,

The present invention provides a cosmetic composition for skin whitening comprising fermented saccharification of turmeric.

The present invention provides a cosmetic composition for preventing or improving skin inflammation comprising a saccharified fermentation product of turmeric.

The turmeric saccharified fermentation product according to the present invention,

Mixing 95-105 parts by weight of dried turmeric based on 2650 parts by weight of 25 to 27 brix sugar water (step 1);

Putting it in a container, sealing it, and anaerobic fermentation at a temperature of 20 to 30 ° C. for 3 to 5 weeks (step 2); and

It can be prepared including; taking the liquid phase of the fermentation product (step 3).

The saccharified fermentation product of turmeric according to the present invention is effective in skin whitening and inflammation improvement.

1 is a graph measuring the amount of melanin produced by treatment concentration of the saccharification and fermentation of turmeric according to Example 1.
Figure 2 is a graph measuring the amount of NO production for each treatment concentration of the saccharification and fermentation of turmeric according to Example 1.

Hereinafter, the present invention will be described in detail.

In the present invention, 'extract' refers to separating and obtaining an extract from active ingredient raw materials using an extraction and separation method known in the art, and the extract defined in the present invention is obtained by using an appropriate solvent as described above. It may be extracted by extracting the active ingredient raw material prepared as it is or by grinding. At this time, as an appropriate solvent that can be used to obtain the extract, any solvent acceptable in the art may be used, and water or an organic solvent may be used. For example, extraction solvents such as water, alcohol having 1 to 5 carbon atoms, hexane, chloroform, and butylene glycol may be used alone or in combination, but are not limited thereto.

As an extraction method, methods such as hot water extraction, cold brew extraction, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, and compression may be used. In addition, the desired extract may be additionally subjected to a conventional fractionation process or may be purified using a conventional purification method. There is no limitation on the preparation method of the extract of the present invention, and any known method may be used.

For example, the extract included in the composition of the present invention may be used as it is while storing the primary extract extracted by the solvent extraction method described above, centrifuged, filtered, and concentrated, then lyophilized and stored in a refrigerator, or the primary extract may be used as it is under reduced pressure. It can be prepared in a powder state by further processes such as distillation and freeze drying or spray drying. In addition, a fraction further purified from the primary extract using various chromatography methods such as silica gel column chromatography, thin layer chromatography, and high performance liquid chromatography. can also be obtained.

Therefore, in the present invention, the extract is a concept that includes all extracts, fractions, and purified products obtained in each step of extraction, fractionation, or purification, and dilutions, concentrates, or dried products thereof.

cosmetic composition

The present invention provides a cosmetic composition containing an active substance.

The cosmetic composition is, for example, a solution, gel, solid or kneaded anhydrous product, emulsion obtained by dispersing an oil phase in an aqueous phase, suspension, microemulsion, microcapsule, microgranule or ionic (liposome), nonionic vesicle dispersant may be in the form of In more specific form, it is provided in the form of lotion, emulsion, cream, toner, lotion, serum, essence, emulsion, powder, cosmetic ointment, spray, gel, pack, cleanser, soap, shampoo, rinse, bath additive, cleanser, or conceal stick. It can be. In addition, it may be prepared in the form of a foam (foam) or the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition includes a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, Water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, humectants, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or commonly used in cosmetics It may contain adjuvants commonly used in the cosmetic field, such as any other ingredients that are

In the cosmetic composition containing the active material of the present invention, the active material of the present invention may be added in an amount of 0.1 to 50% by weight, preferably 1 to 10% by weight, in the cosmetic composition that is usually contained.

When the active substance of the present invention is used as an external skin preparation, additionally, a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, and a surfactant , water, ionic or nonionic emulsifiers, fillers, sequestering agents and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles, or external preparations for skin It may contain adjuvants commonly used in the field of dermatology, such as any other ingredients used as In addition, the components may be introduced in an amount generally used in the field of skin science.

In addition, the cosmetic composition according to the present invention is a serum, lotion, essence, paste, mask pack, patch, gel, cream, lotion, nutrient lotion, nutrient cream, moisture cream, massage cream, powder, soap, cleanser, It can be prepared and used in formulations such as oil, foundation, makeup base, wax, and spray.

Hereinafter, the present invention will be described in more detail by the following examples. However, the following examples are merely illustrative of the present invention, and the contents of the present invention are not limited by the following examples.

<Example 1> Preparation of fermented saccharification of turmeric

Approximately 26 brix of sugar water was prepared by adding 0.65 kg of sugar to 2 L of purified water, and 100 g of dried turmeric was added thereto, sealed, and anaerobic fermentation was carried out for 4 weeks in a storage room maintaining a temperature of about 22 ° C.

After fermentation for 4 weeks, a liquid sample of the fermentation product was obtained using a 100 mesh filter, which was diluted and used in activity evaluation.

<Experimental Example 1> Evaluation of whitening activity

(1) test material

Mouse melanoma B16F10 Cells (ATCC, USA)

DMEM (Dulbecco's Modified Eagle's Medium, Gibco, USA)

FBS (Fetal bovine serum, Gibco, USA)

MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide, Amresco, USA)

Arbutin (Sigma, USA)

NaOH (Sigma, USA)

PBS (Phosphate buffered saline, Gibco, USA)

Melanin (Sigma, USA)

(2) Test method for measuring the amount of melanin inside and outside the cell

B16F10 cells are inoculated into a 6-well plate at a concentration of 1×10 ⁵ cells/well per well, and cultured for 24 hours under cell culture conditions. Thereafter, the medium of each well was removed, washed with PBS, and the test substance for each concentration (the sample of Example 1 was diluted and used in DMEM culture medium) and α-Melanocyte stimulating hormone in DMEM medium without phenol red (α-MSH, final treatment concentration 100 nM) into the culture medium containing 72 hours. Thereafter, the supernatant is taken to measure the amount of extracellular melanin, and the absorbance of free melanin in the medium is measured at 400 nm or 490 nm. After removing the medium for measuring the amount of intracellular melanin, the cells were washed with PBS, dissolved in 1M NaOH at 60 ° C. for 30 minutes, and absorbance was measured at 400 nm or 490 nm. The amount of melanin released in the medium and the amount of intracellular melanin are added together to determine the total amount of melanin, and the total amount of melanin is corrected by the total amount of protein. The solvent control group uses DMEM medium without phenol red instead of the test substance, and the positive control group uses 100 μg/mL of arbutin.

The amount of protein is quantified according to the Bradford method using bovine serum albumin (BSA) as a standard solution. BSA was diluted in distilled water to 0, 2, 5, 10, 20, and 40 μg/mL in stages, and 0.98 mL of Bradford reagent and 20 μL of each concentration of BSA solution were added to a 1.5 mL tube, reacted for 5 minutes, and then 96- Transfer 200 μL to a well plate and measure the absorbance at 595 nm to obtain the BSA standard curve. In the same way as above, measure the absorbance of each cell lysate and the Nradford reagent mixture instead of the BSA solution, and calculate the protein amount using the standard curve obtained from BSA.

All data were expressed as mean value ± standard deviation, and statistical analysis was performed using SPSS ^® Package Program (IBM, USA). All results are the results of three or more independent experiments, and statistical significance is verified at the significance level of p<0.05 by independent samples t-test.

(3) Experimental results

Material Stimulation with a-MSH (100 nM) Concentration (%) Melanin contents (%) saccharification curcuma longa
<Example 1> - 0 67.68 + 0 100.00 0.003 97.32 0.005 96.58 0.01 91.51 0.02 86.62 Arbutin (positive control) 0.01 68.22

The results of Table 1 are illustrated and shown in FIG.

1 is a graph measuring the amount of melanin produced by treatment concentration of the saccharification and fermentation of turmeric according to Example 1.

As shown in Table 1 and FIG. 1, it was confirmed that the amount of melanin produced decreased as the treatment concentration of saccharified turmeric increased.

<Experimental Example 2> Evaluation of anti-inflammatory activity

(1) test material

Mouse macrophages (RAW264.7 cells, ATCC, USA)

DMEM (Dulbecco's Modified Eagle's Medium, Gibco, USA)

FBS (Fetal bovine serum, Gibco, USA)

PBS (Phosphate buffered saline, Gibco, USA)

MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide, Amresco, USA)

Nitric oxide [NO] detection kit (iNtRON, USA)

Dexamethasone (Sigma-Aldrich, USA)

(2) NO production suppression test method

RAW 264.7 cells were inoculated in a 24-well plate at a concentration of 1.8 × 10 ⁵ cells/well using DMEM medium supplemented with 10% FBS, and after culturing for 16 hours, the test substances for each concentration (DMEM culture medium of Example 1) were inoculated. Samples were diluted and used) and 1 μg/mL of LPS were simultaneously treated and incubated for 24 hours. The amount of NO produced is measured by NO ₂ ^- present in the cell culture medium using a Nitric oxide detection kit. In a 96-well plate, mix 100 μL of cell culture supernatant and 50 μL of N1 buffer (substrate solution), react at room temperature for 10 minutes, add 50 μL of N2 buffer, and measure absorbance at 540 nm. The measured NO production amount is corrected by the total protein amount, and as a positive control group, Dexamethasone (final treatment concentration 7.8 μg/mL) is used for comparison.

The amount of protein is quantified according to the Bradford method using bovine serum albumin (BSA) as a standard solution. BSA was diluted in distilled water to 0, 2, 5, 10, 20, and 40 μg/mL in stages, and 0.98 mL of Bradford reagent and 20 μL of each concentration of BSA solution were added to a 1.5 mL tube, reacted for 5 minutes, and then 96- Transfer 200 μL to a well plate and measure the absorbance at 595 nm to obtain the BSA standard curve. In the same way as above, measure the absorbance of each cell lysate and the Nradford reagent mixture instead of the BSA solution, and calculate the protein amount using the standard curve obtained from BSA.

All data were expressed as mean value ± standard deviation, and statistical analysis was performed using SPSS ^® Package Program (IBM, USA). All results are the results of three or more independent experiments, and statistical significance is verified at the significance level of p<0.05 by independent samples t-test.

(3) Experimental results

Material Stimulation with LPS (1 μg/mL) Concentration (%) NO Production (%) saccharification curcuma longa
<Example 1> - 0 1.47 + 0 100.00 0.003 95.61 0.005 94.22 0.01 88.77 0.02 87.54 0.04 84.49 Dexamethasone (Positive control) 0.0008 40.79

The results of Table 2 are illustrated and shown in FIG. 2 .

Figure 2 is a graph measuring the amount of NO production for each treatment concentration of the saccharification and fermentation of turmeric according to Example 1.

As shown in Table 2 and FIG. 2, it was confirmed that the amount of NO production decreased as the treatment concentration of saccharified turmeric increased. Therefore, it was found that saccharified turmeric has an anti-inflammatory effect.

Formulation example of cosmetics

The green tangerine, turmeric, and citrus flower honey extracts according to the present invention can be manufactured into various types of cosmetics depending on the purpose. The following exemplifies a method for preparing some cosmetics containing green tangerine, turmeric, and citrus flower honey extract as an active ingredient, but the present invention is not limited thereto.

<Formulation Example 1> Preparation of softening lotion

Green tangerine, turmeric and citrus flower honey extract 10.0 parts by weight

1,3-butylene glycol 1.00 parts by weight

Disodium EDT 0.05 part by weight

allantoin 0.10 parts by weight

dipotassium glycyrrhizate 0.05 parts by weight

citric acid 0.01 part by weight

sodium citrate 0.02 part by weight

glycereth-26 1.00 parts by weight

Arbutin 2.00 parts by weight

Hydrogenated Castor Oil 1.00 parts by weight

ethanol 30.0 parts by weight

preservative a very small amount

coloring agent a very small amount

flavoring agent a very small amount

Purified water balance

<Formulation Example 2> Preparation of nutrient cream

Green tangerine, turmeric and citrus flower honey extract 10.0 parts by weight

1,3-butylene glycol 7.00 parts by weight

glycerin 1.00 parts by weight

D-panthenol 0.10 part by weight

plant extract 3.20 parts by weight

Magnesium aluminum silicate 0.30 part by weight

PEG-40 Stearate 1.20 parts by weight

stearic acid 2.00 parts by weight

polysorbate 60 1.50 parts by weight

Lipophilic Glyceryl Stearate 2.00 parts by weight

Sorbitan sesquioleate 1.50 parts by weight

Cetearyl Alcohol 3.00 parts by weight

mineral oil 4.00 parts by weight

squalane 3.80 parts by weight

Carrylic/capric triglycerides 2.80 parts by weight

vegetable oil 1.80 parts by weight

dimethicone 0.40 parts by weight

Diposium glycyrrhizate a very small amount

allantoin a very small amount

sodium hyaluronate a very small amount

Tocopheryl Acetate Appropriate amount

triethanolamine Appropriate amount

preservative Appropriate amount

flavoring agent Appropriate amount

Purified water balance

So far, the present invention has been looked at with respect to its preferred embodiments. Those skilled in the art to which the present invention pertains will be able to understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative rather than a limiting point of view. The scope of the present invention is shown in particular in the claims rather than the foregoing description, and all differences within the equivalent range will be construed as being included in the present invention.

Claims (4)

A cosmetic composition for skin whitening comprising fermented saccharification of turmeric.
A cosmetic composition for preventing or improving skin inflammation comprising a saccharified fermentation product of turmeric.
According to claim 1 or 2,
The turmeric saccharified fermentation product,
Mixing 95-105 parts by weight of dried turmeric based on 2650 parts by weight of 25 to 27 brix sugar water (step 1);
Putting it in a container, sealing it, and anaerobic fermentation at a temperature of 20 to 30 ° C. for 3 to 5 weeks (step 2); and
A cosmetic composition comprising the step of taking the liquid phase of the fermented product (step 3).
According to claim 1 or 2,
Any one selected from the group consisting of lotion, emulsion, cream, toner, lotion, serum, essence, emulsion, powder, cosmetic ointment, spray, gel, pack, cleanser, soap, shampoo, rinse, bath additive, cleanser, and conceal stick Characterized in that prepared in a formulation, a cosmetic composition.

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