KR20230067053A - cosmetic composition for skin whitening and antiwrinkle - Google Patents

Abstract

The present invention relates to a cosmetic composition with whitening and wrinkle alleviating functions, and more specifically, to a functional cosmetic composition that uses natural materials and has excellent whitening, wrinkle alleviating, antioxidant, anti-inflammatory, and antibacterial effects. The cosmetic composition having the whitening and wrinkle alleviating functions of the present invention includes a complex extract of a propolis extract, a Rhus chinensis extract, a Boswellia extract, a pineapple extract, an Actinidia chinensis extract, and a pear extract.

Description

Cosmetic composition having whitening and wrinkle improvement functions {cosmetic composition for skin whitening and antiwrinkle}

The present invention relates to a cosmetic composition having whitening and anti-wrinkle functions, and more particularly, to a functional cosmetic composition having excellent whitening and anti-wrinkle, antioxidant, anti-inflammatory, and antibacterial effects using natural materials.

The skin is the largest organ in the body, always in direct contact with the external environment, and plays a role as a protective film to protect the living body from various stimuli or dry environments. In addition, it plays a very important role in protecting the body from physical abrasions, preventing loss of moisture inside the body, protecting from ultraviolet rays generated by the sun, and regulating body temperature.

Such skin causes skin abnormalities such as skin contamination, dryness, trouble, and pigmentation due to various physical factors, external environmental factors, and infections, and eventually causes dermatitis or allergy to occur and deteriorate.

This skin tissue is largely divided into three parts, that is, epidermis, dermis and subcutaneous tissue. Human skin color appears differently depending on the number, size, type, and distribution of melanosomes containing melanin contained in skin cells. Melanosomes are produced by melanocytes, and melanin is a black pigment produced in the epidermis and is produced in cells called melanocytes.

Various factors are involved in determining the skin color of a person. Among them, factors such as the activity of melanocytes that produce melanin pigment, the distribution of blood vessels, the thickness of the skin, and the presence or absence of pigments inside and outside the human body such as carotenoids and bilirubin are important. In particular, the most important factor is a black pigment called melanin, which is produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. The formation of this melanin pigment is influenced by genetic factors, hormone secretion, physiological factors related to stress, and the like, and environmental factors such as ultraviolet irradiation. However, excessive production and deposition of melanin pigment causes skin diseases such as darkening of the skin, melasma, freckles, and abnormal skin pigmentation such as pigmentation.

In addition, as the skin ages, the thickness of the epidermis, dermis, and subcutaneous tissue becomes thinner, and the skin barrier function deteriorates. do. In particular, highly reactive active oxygen and free radicals generated due to the increase in the amount of ultraviolet rays, air pollution, and excessive fatigue and stress of modern people oxidize or denature biological components (eg, proteins, nucleic acids, cell membrane lipids, etc.), It acts as a major cause of skin aging. Therefore, various functional cosmetics are being developed to prevent and treat wrinkle formation, skin elasticity reduction, pigmentation, melasma, freckles and dryness due to skin aging.

Recently, an increase in income due to rapid economic development has improved the quality of life, but due to industrialization, the risk of skin irritation due to air pollution and environmental pollution is gradually increasing. For this reason, interest in healthy skin is increasing. There is a trend toward eco-friendly and natural cosmetics made from natural ingredients according to the purchasing intention of consumers who want healthy and beautiful skin. Functional cosmetics of natural origin are more emphasized on functionality and safety than general cosmetics.

As disclosed in Korean Patent Publication No. 10-2018-0123831, Korean Patent Publication No. 10-2019-0093283, and Korean Patent Registration No. 10-2021264, natural products derived from plants or marine organisms are applied to cosmetics in various forms. . In particular, natural cosmetics and herbal cosmetics developed and manufactured with natural herbal ingredients in pursuit of naturalism are leading the trend. In general, medicinal plants are effective in promoting health, preventing diseases, and improving health.

1. Republic of Korea Patent Publication No. 10-2018-0123831: Functional cosmetic composition containing herb extract and portulaca extract and its manufacturing method 2. Republic of Korea Patent Publication No. 10-2019-0093283: Multi-functional cosmetic composition composed of Golcheobbo extract and cosmetics containing the same 3. Korean Patent Registration No. 10-2021264: Functional cosmetic composition for skin regeneration containing active ingredients extracted and separated from seaweed

Accordingly, an object of the present invention is to provide a functional cosmetic composition having whitening and wrinkle improvement, antioxidant effect, anti-inflammatory effect, and antibacterial effect by utilizing natural materials as raw materials of cosmetics.

The cosmetic composition having whitening and anti-wrinkle functions of the present invention for achieving the above object contains a complex extract in which propolis extract, gall nut extract, boswellia extract, pineapple extract, kiwi extract, and pear extract are mixed.

Each extract is extracted by adding an extraction solvent, and the extraction solvent is at least one selected from water, lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols, or mixtures thereof.

The composite extract contains 45 to 55 parts by weight of the nut gall extract, 35 to 45 parts by weight of the Boswellia extract, 20 to 30 parts by weight of the pineapple extract, and 20 to 30 parts by weight of the kiwi extract, based on 100 parts by weight of the propolis extract. , 10 to 15 parts by weight of the pear extract is mixed.

The composite extract has antioxidant activity.

The composite extract has anti-inflammatory activity.

The composite extract has antibacterial activity.

The cosmetic composition is any one formulation selected from lotion, lotion, cream, essence, pack, powder, makeup base, foundation, body oil, body lotion, cleansing oil, cleansing foam, hair oil, hair shampoo, hair rinse, and soap is formed by

It further contains a fermented vegetable extract extracted by fermenting the leaves of the vegetable.

As described above, the present invention can provide a cosmetic composition that is safe and has excellent functionality by using natural materials such as propolis, gall nut, boswellia, pineapple, kiwi, and pear as materials for cosmetics.

The cosmetic composition of the present invention contains a complex extract obtained by mixing extracts of propolis, nut gall, boswellia, pineapple, quinoa, and pear, and has excellent whitening and anti-wrinkle effects, antioxidant effects, anti-inflammatory effects, and antibacterial effects.

1 and 2 are graphs showing the antioxidant activity test results,
3 and 4 are graphs showing the whitening activity test results,
5 and 6 are graphs showing the results of cytotoxicity experiments;
7 is a graph showing the results of wrinkle improvement experiments;
8 is a graph showing the anti-inflammatory test results,
9 and 10 are graphs showing additional antioxidant activity test results.

Hereinafter, a cosmetic composition having whitening and anti-wrinkle functions according to a preferred embodiment of the present invention will be described in detail.

The cosmetic composition of the present invention contains a complex extract in which propolis extract, gall nut extract, boswellia extract, pineapple extract, kiwi extract, and pear extract are mixed.

Propolis is a substance made by mixing beeswax and resin substances collected by bees from salivary gland secretions and trees, flowers, leaves, etc., and is used to protect houses from bacterial or viral infections.

Propolis consists of about 200 compounds, flavonoids, minerals, vitamins, amino acids, fats, and organic acids. Among them, flavonoids are contained in various parts of plant stems and flowers, and are light yellow and yellow phenolic compounds. Flavonoids are known as the most important component of propolis that enhances immunity in vivo and exhibits physiologically active functions such as antiviral and anticancer properties.

Galla rhois is a deciduous arboretum belonging to the sumacaceae, a wormwood ( Rhus javanica L.) or an insect nest formed by the cuts of galla aphids on the leaves of other plants of the same genus. It has a high content of tannin, which is a major component, and shows antidiarrheal, antibacterial, and glandular secretion suppression effects by astringent action. Gallbladder has been reported to have pharmacological effects such as antibacterial, antioxidant, antithrombotic, and hepatoprotective effects.

Boswellia is a medicine made by drying the sap of the frankincense tree belonging to the Olive family. It is recorded in traditional Indian Ayurvedic medicine that it was used for chronic inflammation, arthritis, anti-inflammatory, bruises, respiratory diseases and diarrhea, and has high anti-inflammatory pain relief. It is known that it has been continuously used for pain relief and inflammation treatment due to its effectiveness. The main components of boswellia are boswellic acid and terpenoid-type antioxidants, among which boswellic acid is a functional substance that exhibits anti-inflammatory and analgesic effects of boswellia.

Pineapple ( Ananas comosus ) is a fruit native to Central and South America belonging to the pineapple family (Bromeliaceae). The water content of pineapple flesh is about 80-85%, and sugar is mainly sugar, including fructose and glucose, which is 12-15%. It also contains 0.6-1.2% acid, of which about 87% is citric acid and the rest is malic acid. In addition, it contains about 0.5 to 1% of protein, 0.1% of fat, and some fiber, minerals such as calcium, phosphorus, and iron, and vitamins A, B, and C.

Pineapple bromelain is a thiol-based proteolytic enzyme with -SH group. It has been used as an auxiliary digestive and anti-inflammatory agent since the Middle Ages. It is known that no tolerance develops.

Kiwi, also called kiwi, is rich in vitamin C, vitamin E and fiber, and is known to have a high blood pressure preventive effect because it is low in sodium and high in potassium. In addition, quinic acid, malic acid, citric acid, etc. are contained in a large amount of organic acids, so it has a unique flavor. It contains proteolytic enzymes, so adding a few pieces of kiwi to meat dishes at home or in restaurants improves the color and aroma, and is known to prevent various diseases caused by excessive consumption of meat.

Pear is a fruit of a deciduous plant belonging to the genus Pyrus, and is a representative alkaline food that has a higher sugar content than other fruits and contains a lot of inorganic components. The peel containing invertase and oxytase has been used for boils and skin diseases, and the fruit has been used for phlegm, cough, hangover, fever relief, bowel movements, and soft meat.

The cell wall of the pear is composed of polysaccharides such as cellulose, hemicellulose, and pectin, glycoproteins, and trace amounts of phenolic substances. Pears are known to have excellent intestinal function due to their high dietary fiber content and have excellent antioxidant, anticancer and anti-inflammatory effects because they contain a large amount of phenolic substances, which are functional ingredients. In addition, pear polyphenols are reported to be excellent in lowering blood cholesterol, enhancing immune function, and inhibiting the growth of cancer cells.

The above-mentioned natural materials such as propolis, nut gall, boswellia, pineapple, kiwi, and pear are used in the form of extracts.

Each extract can be obtained by adding an extraction solvent. At this time, at least one selected from water, lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols, or mixtures thereof may be used as the extraction solvent.

Methanol, ethanol, etc. can be used as a lower alcohol having 1 to 4 carbon atoms, and butylene glycol, propylene glycol, pentylene glycol, etc. can be used as a polyhydric alcohol. As the mixture, a mixture of water and a lower alcohol, a mixture of water and a polyhydric alcohol, a mixture of a lower alcohol and a polyhydric alcohol, or a mixture of water, a lower alcohol and a polyhydric alcohol may be used.

As an example of extraction, after adding an extraction solvent in a weight ratio of 2 to 20 times for each natural material, extraction may be performed by hot water extraction, cold extraction, or warm needle extraction at 10 to 150 ° C. for 2 to 200 hours. As a specific example of extraction, propolis extract may be obtained by adding ethanol to propolis and extracting at 30 to 40° C. for 100 to 140 hours.

In addition to the above-mentioned extraction method, it can be extracted using reflux cooling extraction, ultrasonic extraction method, and the like. In addition, an extract subjected to a conventional purification process is also included. For example, an active fraction obtained through various additional purification methods, such as separation using an ultrafiltration membrane having a certain molecular weight cut-off value and separation by various chromatography, is also included in the extract.

In addition, of course, the extract extracted using the extraction solvent can be obtained in powder form through filtration, concentration under reduced pressure, freeze-drying, spray drying, or the like.

Propolis extract extracted from propolis, nut gall extract extracted from nut gall, boswellia extract extracted from boswellia, pineapple extract extracted from pineapple fruit, kiwi fruit extract extracted from kiwi fruit, and pear extract extracted from pear fruit were mixed. Get a complex extract.

These complex extracts improve whitening and wrinkles. In addition, the complex extract has antioxidant activity, anti-inflammatory activity, and antibacterial activity. Therefore, the cosmetic composition of the present invention containing the composite extract can provide cosmetics with excellent functionality.

The present invention may contain 0.01 to 20% by weight of the composite extract based on the total weight of the cosmetic composition. If the content of the complex extract is less than 0.01% by weight, the whitening and wrinkle improvement, antioxidant effect, anti-inflammatory effect, and antibacterial effect are insignificant, and when it exceeds 20% by weight, it is difficult to have an increased effect compared to the content.

The cosmetic composition of the present invention is prepared by adding various known ingredients using a method known in the art to make lotion, lotion, cream, essence, pack, powder, makeup base, foundation, body oil, body lotion, cleansing oil, It may be prepared in any one formulation selected from cleansing foam, hair oil, hair shampoo and hair rinse, and soap.

Specifically, when the formulation of the cosmetic composition of the present invention is a paste, cream, gel, or pack, animal fibers, vegetable fibers, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, Silica, talc or zinc oxide or the like may be used.

When the formulation of the cosmetic composition of the present invention is a powder, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or non-radiative acid ester of sorbitan may be used.

When the formulation of the cosmetic composition of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tragacanth and the like may be used.

When the formulation of the cosmetic composition of the present invention is a surfactant-containing detergent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate , Alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil or ethoxylated glycerol fatty acid ester, etc. may be used.

If the formulation of the cosmetic composition of the present invention is an essence, herb water, thickener, adenosine, moisturizer, hyaluronic acid, trehalose, squalane, preservatives, essential oil and the like may be used.

In addition, the cosmetic composition of the present invention can use conventionally known ingredients such as stabilizers, solubilizers, vitamins, pigments, and fragrances, and can be prepared in any formulation conventionally prepared in the art, can be selected appropriately.

As an example of the formulation, the toner contains 1.5% by weight of ethanol, 1.0% by weight of natural liquid, 3.0% by weight of betaine, 3.0% by weight of DL Panthenol, 1.0% by weight of Sebum-A, 2.0% by weight of arbutin paste, 2.0% by weight of anenosine, and eco gum. It may be composed of 0.03% by weight, 4.0% by weight of glycerin, 1.0% by weight of collagen, 10.0% by weight of complex extract, preservatives (a small amount), and purified water (remaining amount).

In addition, as an example of the formulation, the lotion contains carbomer 1.0% by weight, dimethicone 2.0% by weight, jojoba oil 5.0% by weight, provitamin E 0.5% by weight, olive emulsifying wax 1.0% by weight, arbutin paste 5.0% by weight, T-Mixture 1.2% by weight, foam mixture 1.0% by weight, bartain 1.0% by weight, cetearyl alcohol 1.5% by weight, glycerin 4.0% by weight, flex oil 5.0% by weight, arbutin paste 5.0% by weight, complex extract 10.0% by weight, blending essence It can be composed of oil (trace amount), preservative (trace amount), and purified water (remaining amount).

In addition, as an example of the formulation, the cream contains 1.5% by weight of cetearyl alcohol, 1.0% by weight of glyceryl stearate, 1.0% by weight of polysorbate 60, 0.3% by weight of sorbitan sesquioleate, 6.0% by weight of cetyloctanoate, Squalane 8.0% by weight, apricot kernel oil 4.0% by weight, dimethicone 2.0% by weight, butylene glycol 4.0% by weight, glycerin 4.0% by weight, magnesium aluminum silicate 0.4% by weight, complex extract 10.0% by weight, blending essence oil ( It can be composed of a trace amount), preservatives (trace amount), and purified water (remaining amount).

In addition, as an example of the formulation, the essence is ethanol 1.5% by weight, natural liquid 1.0% by weight, carbomer 1.0% by weight, betaine 3.0% by weight, adenosine 2.0% by weight, arbutin paste 5.0% by weight, sodium hyaluronate aqueous solution (1%) 0.03% by weight, DL Panthenol 3.0% by weight, glycerin 4.0% by weight, Sebum-A 1.0% by weight, complex extract 10.0% by weight, blending essence oil (trace amount), preservative (trace amount), purified water (remaining amount).

In addition, as an example of the formulation, the foundation contains 2.0% by weight of stearic acid, 0.5% by weight of cetearyl alcohol, 1.0% by weight of glyceryl stearate, 0.5% by weight of polysorbate 60, 0.7% by weight of sorbitan sesquioleate, and hydrogel. Genated Labseed Oil 2.0% by weight, sunflower oil 5.0% by weight, squalane 6.0% by weight, cetyloctanoate 4.0% by weight, cyclomethicone 2.0% by weight, butylene glycol 5.0% by weight, glycerin 3.0% by weight, magnesium aluminum Silicate 0.6% by weight, xanthan gum 0.05% by weight, titanium dioxide 10.0% by weight, talc 3.0% by weight, hydrated ferric oxide 1.3% by weight, ferric oxide 0.3% by weight, ferrus-ferric oxide 0.15% by weight, complex extract 10.0% by weight %, preservatives (trace amount), and purified water (remaining amount).

In addition, as an example of the formulation, the pack contains 0.5% by weight of propylene glycol, 1.0% by weight of natural liquid, 3.0% by weight of polyvinyl alcohol, 3.0% by weight of ethanol, 1.0% by weight of carbomer, 2.0% by weight of collagen, 1.0% by weight of eco gum, and peptides. RF 1.0 wt%, DL Panthenol 3.0 wt%, Glycerin 4.0 wt%, Sebum-A 1.0 wt%, Adenosine 2.0 wt%, Complex Extract 10.0 wt%, blending essence oil (trace amount), preservative (trace amount), purified water (remaining amount) can be composed of

In addition, as an example of the formulation, the hair shampoo contains 0.5% by weight of polyquaternium, 1.0% by weight of natural liquid, 3.0% by weight of cocamido propyl betaine, 3.0% by weight of betaine, 7.0% by weight of CDA, 25.0% by weight of LES, and cocobetaine. 5.0% by weight, collagen 1.0% by weight, DL Panthenol 3.0% by weight, glycerin 4.0% by weight, Sebum-A 1.0% by weight, adenosine 2.0% by weight, complex extract 10.0% by weight, blending essence oil (trace amount), preservative (trace amount), It can be composed of purified water (remaining amount).

In addition, as an example of the formulation, the soap may be composed of 10.0% by weight of the complex extract, 2.0% by weight of glycerin, 1.0% by weight of essence cell oil, and a soap base (remaining amount) obtained by saponifying fatty acids.

On the other hand, the present invention may contain a fermented zucchini extract together with a complex extract as another example. In this case, 2 to 30 parts by weight of the fermented zucchini extract may be mixed with respect to 100 parts by weight of the complex extract.

Jichae fermentation extract can be obtained by extracting after fermenting Jichae. Triglochin maritimum L. is a perennial herb belonging to the Triglochinaceae family and grows mainly in coastal tidal flats.

The whole part or only the leaves can be used for the support. As a method for fermenting the ground, 20 to 50 parts by weight of sugar is mixed with respect to 100 parts by weight of the ground, 0.01 to 1.0 parts by weight of the fermentation strain is added, and then fermented at 20 to 40 ° C. for 20 to 60 days. A strain of the genus Saccharomyces may be used as a fermentation strain. Examples of Saccharomyces genus strains include Saccharomyces rouxii , Saccharomyces cerevisiae , Saccharomyces oviformis , Saccharomyces steineri and the like. can Commercially available dried yeast products can be used as yeast.

When fermentation is complete, water is added to the fermented product to extract the fermented zucchini extract. As an example of extraction, a method of vacuum extraction at low temperature may be used. For example, 10 to 50 parts by weight of fermented material is added to a vacuum low-temperature extractor based on 100 parts by weight of water, and then vacuum extraction is performed at 60 to 80 ° C. for 4 to 10 hours under vacuum (100 to 200 torr) conditions. After vacuum low-temperature extraction, it can be filtered to obtain a fermented zucchini extract.

The fermented zucchini extract obtained in this way has excellent antioxidant and antibacterial activity, so that the functionality of the cosmetic composition can be improved. In particular, it is expected that the fermented extract of jjichae will be added to the complex extract to increase the effect of the complex extract.

When containing the fermented zucchini extract, the cosmetic composition of the present invention may contain 0.01 to 20% by weight of the complex extract and 0.1 to 5% by weight of the fermented zucchini extract based on the total total weight.

Hereinafter, the contents of the present invention will be specifically described through the following examples and experiments. This is for explaining the present invention in more detail, and the scope of the present invention is not limited to the following examples.

(Example: Complex extract extraction)

- Propolis extract was prepared by adding 2L of 20% (w/w) ethanol to 500 g of propolis, extracting at 50 rpm and 35 ° C for 5 days, and then filtering.

- Wash 500g of Boswellia with water, add 2L of 20% (w/w) ethanol, extract at 50rpm and 35°C for 5 days, and then filter to prepare Boswellia extract.

- After washing 500g of pear fruit with water, 2L of 20% (w/w) ethanol was added, extracted at 50 rpm and 35 ° C for 5 days, and then filtered to prepare a pear extract.

-After washing 500g of nut gall with water, 2L of 20% fermented alcohol was added, extracted at 50 rpm and 35 ° C for 5 days, and filtered to prepare nut gall extract.

-Pineapple extract was prepared by adding 2L of 20% (w/w) ethanol to 500g of fruit pulp from which the fruit skin was removed, extracting at 50rpm and 35℃ for 5 days, and then filtering.

-After washing 500g of kiwifruit fruit with water, 2L of 20% (w/w) ethanol was added, extracted at 50 rpm and 35℃ for 5 days, and then filtered to prepare an extract of kiwifruit.

Each extract was mixed in the ratio shown in Table 1 below to obtain 6 types of composite extracts (No.1 to 6). And for comparison, a propolis alone extract (No.7) and an extract (No.8) obtained by mixing propolis extract and nut gall extract at the same weight ratio were used as extracts.

propolis
extract (g) gallnut
extract (g) boswellia
extract (g) pineapple
extract (g) kiwifruit
extract (g) ship
extract (g) No. One 50 10 10 10 10 10 No. 2 40 20 15 10 10 5 No. 3 30 20 20 15 10 5 No. 4 20 20 20 20 10 10 No. 5 10 10 10 10 30 30 No. 6 10 10 10 20 25 25 No. 7 100 0 0 0 0 0 No. 8 50 50 0 0 0 0

The extracts of No. 1 to 8 were aged for 3 days at 45 ± 2 ° C in the dark, filtered with filter paper, lyophilized, prepared in powder form, and used as samples. Each sample was diluted to a concentration of 25, 50, 100, or 200 μg/ml.

<Experimental example>

1. Antioxidant activity test

Antioxidant test was conducted through DPPH assay and ABTS assay.

The results of measuring the free radical scavenging activity using DPPH (1,1-diphenyl-2-picryl-hydrazyl) assay are shown in FIG. 1 . BHA (butylated hydroxyl anisol) was used as a positive control.

Referring to Figure 1, complex extracts No. 1 and No. 2 were higher than No. 7 using propolis alone, and in particular, complex extract No. 2 showed the highest antioxidant activity.

Antioxidant test results using ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) are shown in FIG. 2 . BHA (butylated hydroxyl anisol) was used as a positive control.

Referring to Figure 2, if it came out in a pattern similar to that confirmed through the DPPH method showed the highest activity in the composite extract No. 2.

2. Whitening activity test

In order to find out the efficacy as a raw material for cosmetics, the activity related to whitening was measured. Whitening-related activity was performed by Tyrosinase inhibition activity assay (L-DOPA oxidation inhibition activity assay).

To measure tyrosinase inhibitory activity, mushroom tyrosinase and substrate L-DOPA were used. For the reaction zone, 0.2 mL of a substrate solution obtained by dissolving 10 mM L-DOPA in 0.5 mL of 0.175 M sodium phosphate buffer (pH 6.8) and arbutin were used as a composite extract or positive control. 0.2 mL of mushroom tyrosinase (110 U/mL) was added to the mixed solution and reacted at 25 ° C for 2 minutes. The absorbance of DOPA chrome generated in the reaction solution was measured at 475 nm. It was expressed as the rate of decrease in absorbance between the sphere and the non-additive sphere. The experimental results are shown in FIG. 3 .

Referring to Figure 3, the whitening activity test results of the composite extract showed a pattern similar to the above antioxidant activity test results. Complex extract No. 2 showed the highest activity.

In order to further confirm the whitening activity of the complex extract, the amount of melanin produced in melanocytes was measured by measuring the intracellular melanin content of the complex extracts from No. 1 to No. 6. 1 × 10 ⁵ B16/F10 cells were dispensed into a cell culture dish and cultured for 24 hours. After sample treatment for 3 days, the cultured cells were washed with 1 × phosphate buffered saline (PBS) and then mixed with 10% dimethyl sulfoxide (DMSO). After treating the included 1 N sodium hydroxide (NaOH) solution and reacting at 80 ° C for one hour, the absorbance was measured at 475 nm using a multi-plate reader. After measuring the total amount of protein, the amount of melanin per certain amount of protein was calculated.

α-MSH (Alpha-Melanocyte-stimulating hormone) was treated to promote intracellular melanin production, and Arb (arbutin) was treated and compared as a positive control. The experimental results are shown in FIG. 4 .

Referring to Figure 4, the composite extract was found to inhibit intracellular melanin synthesis as a whole, showed the most excellent efficacy in the composite extract No. 2 among them.

3. Cytotoxicity test

MTT assay was performed to confirm the cytotoxicity of the composite extracts from No. 1 to No. 6. B16/F10 (Mouse/Melanoma) and Raw264.7 (Mouse/Macrophage) cells were cultured in DMEM medium containing 1% penicillin/streptomycin and 10% fetal bovine serum. ℃, 5% CO ₂ It was cultured in an incubator maintained. Toxicity of the extract to cells was evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. MTT is a pale yellow substrate that reacts with enzymes in the mitochondria of living cells to form formazan. By dissolving this formazan in dimethyl sulfoxide (DMSO) and measuring the absorbance at 570 nm, cell viability was determined. was measured.

The experimental results for B16/F10 cells are shown in FIG. 5 and the experimental results for Raw264.7 cells are shown in FIG. 6 , respectively.

5 and 6, even at the highest concentration of 100 μg / ml of the treatment concentration of the composite extract, most showed a survival rate of 90% or more, and no particular cytotoxicity was observed.

4. Wrinkle improvement experiment

In order to investigate the anti-wrinkle activity of the complex extract, the anti-wrinkle effect was confirmed through elastase inhibition assay. Using n-succinyl-(L-Ala)3-p-nitroanilide as a substrate, the amount of p-nitroanilide produced from the substrate was measured at 37°C for 30 minutes. Prepare each sample to a certain concentration (25, 50, 100 μg/ml), take 40 μl each in a 96-well plate, and add 40 μl of a 2.5 U/ml porcine pancreas elastase solution dissolved in 50 mM Tris-HCl buffer (pH 8.6). . As a substrate, n-succinyl-(L-Ala)3-pnitroanilide (0.5 mg/ml) dissolved in 50 mM Tris-HCl buffer (pH 8.6) was added, reacted for 30 minutes, and left on ice for 5 minutes to stop the reaction. After that, absorbance was measured at 410 nm. Ursolic acid (U-A) was used as a positive control for the wrinkle improvement activity evaluation.

The experimental results are shown in FIG. 7 .

Referring to FIG. 7, the comparative extracts No. 7 and No. 8 showed slightly higher activity, but the composite extract No. 2 showed the highest activity.

5. Anti-inflammatory test

Griess assay was performed to measure anti-inflammatory activity. Raw264.7 cells were treated with 500 ng/ml of LPS to induce an inflammatory response, and mixed aged extracts were treated by concentration and cultured for 24 hours. 0.1% naphylethylenediamine in 2.5% phosphoric acid), and the results are shown in FIG. 8 .

Referring to FIG. 8, when Raw264.7 cells were treated with LPS, an inflammatory reaction was induced to increase NO production, but it was confirmed that NO production was reduced when the composite extract was treated. In particular, it was found to show the highest activity in complex extract No. 2.

6. Antibacterial activity test

Escherichia coli , Staphylococcus aureus, Pseudomonas aeruginosa , Propionibacterium acnes and Staphylococcus epidermidis by paper disk diffusion method Antibacterial activity against was measured. The antibacterial activity test results are shown in Table 2 below.

Antibacterial activity was classified according to the size of the clean jone as shown in Table 2 below.

7-8mm - 8-11mm + 11-14mm ++ 14-17mm +++ 17-20mm ++++ 20 mm > +++++

The antibacterial activity test results are shown in Table 3 below. As a control, phenoxyethanol (PE), a preservative, was used.

E. coli S. aureus P. aeruginosa P. acnes S. epidermidis Non - - - - - PE +++++ +++++ ++++ +++++ +++++ No.1 ++++ +++ ++ +++ ++ No.2 ++++ +++ +++ +++ +++ No. 3 +++ ++ ++ ++ ++ No.4 +++ + + + + No.5 + + + + + No.6 + + + + +

Referring to the results of Table 3, antibacterial activity was shown in complex extracts 1 to 3, and in particular, antibacterial activity against all bacteria was high in No. 2.

<Additional antioxidant activity test>

Through the above experiment, the antioxidant activity of the No. 2 complex extract and each single extract (propolis extract, gall nut extract, boswellia extract, pineapple extract, kiwi extract, and pear extract), which had the highest effect, was tested. The experimental results are shown in FIGS. 9 and 10, respectively. 9 and 10, Pr: propolis extract, Ga: nut gall extract, Bo: boswellia extract, Pi: pineapple extract, Ki: kiwi extract, Pe: pear extract.

Referring to Figure 9, as a result of confirming the antioxidant activity by DPPH, the antioxidant activity of the complex extract No. 2 was higher than that of the single extract. And referring to FIG. 10, as a result of confirming the antioxidant activity with ABTS, the antioxidant activity of the complex extract No. 2 was higher than that of the single extract.

Through the above additional antioxidant activity experiments, it was confirmed that the antioxidant activity was greatly increased by synergistic effect when propolis extract, gall nut extract, boswellia extract, pineapple extract, kiwi extract, and pear extract were mixed.

In view of the above experimental results, the complex extract was found to have excellent antibacterial, antioxidant, anti-inflammatory, whitening and anti-wrinkle effects.

<Extraction of fermented vegetable extract>

40 g of sugar was mixed with 100 g of the leaves of the zucchini collected from the coast of Jeollanam-do, and 0.2 g of Saccharomyces cerevisiae was added as a fermentation strain, followed by fermentation at 30 ° C. for 40 days. After adding 300 g of water to the fermented product, vacuum extraction was performed at 70° C. for 8 hours under vacuum (150 torr). After vacuum extraction, the mixture was filtered with a filter paper to obtain a fermented zucchini extract.

The antibacterial activity was tested by mixing the complex extract of No. 2 and the fermented zucchini extract at a ratio of 4:1. The results are shown in 4 below.

E. coli S. aureus P. aeruginosa P. acnes S. epidermidis Non - - - - - PE +++++ +++++ ++++ +++++ +++++ No. 2 + treasury ++++ ++++ +++ ++++ +++ No.2 ++++ +++ +++ +++ +++

Referring to the results of Table 4 above, it was confirmed that the antibacterial activity was increased when the fermented vegetable extract was mixed with the complex extract No. 2.

(Example: Manufacturing cosmetic composition)

The first essence sample contains 1.5% by weight of ethanol, 1.0% by weight of natural liquid, 1.0% by weight of carbomer, 3.0% by weight of betaine, 2.0% by weight of adenosine, 5.0% by weight of arbutin paste, 0.03% by weight of sodium hyaluronate aqueous solution, and 3.0% by weight of DL panthenol. It was composed of 4.0% by weight of glycerin, 1.0% by weight of Sebum-A, 10.0% by weight of complex extract, 0.4% by weight of blending essential oil, and purified water (remaining amount).

And the second essence sample contains 1.5% by weight of ethanol, 1.0% by weight of natural liquid, 1.0% by weight of carbomer, 3.0% by weight of betaine, 2.0% by weight of adenosine, 5.0% by weight of arbutin paste, 0.03% by weight of sodium hyaluronate aqueous solution, and DL panthenol. It was composed of 3.0% by weight, 4.0% by weight of glycerin, 1.0% by weight of Sebum-A, 8.0% by weight of complex extract, 2.0% by weight of fermented zucchini extract, 0.4% by weight of blending essential oil, and purified water (remaining amount).

A skin test was conducted using the first essence sample and the second essence sample to determine the degree of skin irritation. 24h single epicutaneous patch, a standardized safety evaluation method in which a special patch (IQ chamber, Chemotechnique, Sweden) containing an essence sample is attached to the test subject's back for skin testing, then removed after 24 hours and read after 30 minutes performed as a test. The degree of response of the skin was determined as 1 to 4 points according to the ICDRG (international contact dermatitis research group) evaluation standard table for erythema, edema, scabs and shallow ulcers, and recorded as the total average value. The skin test results are shown in Table 5 below.

The probability of skin irritation is a case where 20% of the total test subjects had a skin reaction of 2 or higher based on the average score, and the average score was 45 or higher (ICDRG score index 0-15: no skin irritation, 15-25: skin irritation Mild erythema at a level without erythema, 25 to 45: Slight erythema and edema require additional confirmation tests, >45: Probability of skin irritation).

division skin irritation 1st essence sample <1.5 2nd essence sample <1.5

From the results of Table 5 above, it was confirmed that both types of essence samples did not show erythema or edema reactions that would cause significant skin irritation to the test subjects. Therefore, it was confirmed that the cosmetic composition of the present invention can be safely used without irritation to the skin.

In the above, the present invention has been described with reference to one embodiment, but this is only exemplary, and those skilled in the art will understand that various modifications and equivalent embodiments are possible therefrom. Therefore, the true protection scope of the present invention should be defined only by the appended claims.

Claims (8)

A cosmetic composition having whitening and anti-wrinkle functions, characterized in that it contains a complex extract obtained by mixing propolis extract, gall nut extract, boswellia extract, pineapple extract, kiwifruit extract, and pear extract. The method of claim 1, wherein each extract is extracted by adding an extraction solvent,
The extraction solvent is a cosmetic composition having a whitening and anti-wrinkle function, characterized in that at least one selected from water, lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols, or mixtures thereof. The method of claim 1, wherein the complex extract is 45 to 55 parts by weight of the nut gall extract, 35 to 45 parts by weight of the Boswellia extract, 20 to 30 parts by weight of the pineapple extract, and the quinoa extract, based on 100 parts by weight of the propolis extract. 20 to 30 parts by weight of the extract, 10 to 15 parts by weight of the pear extract, a cosmetic composition having a whitening and anti-wrinkle function, characterized in that mixed. The cosmetic composition having whitening and anti-wrinkle functions according to claim 1, wherein the composite extract has antioxidant activity. The cosmetic composition according to claim 1, wherein the composite extract has anti-inflammatory activity. The cosmetic composition according to claim 1, wherein the composite extract has antibacterial activity. The cosmetic composition according to claim 1, wherein the cosmetic composition is cosmetic water, lotion, cream, essence, pack, powder, makeup base, foundation, body oil, body lotion, cleansing oil, cleansing foam, hair oil, hair shampoo, hair rinse, soap A cosmetic composition having whitening and anti-wrinkle functions, characterized in that it is formed of any one formulation selected from among. According to claim 1, wherein the cosmetic composition having a whitening and anti-wrinkle function, characterized in that it further contains a fermented zucchini extract extracted by fermenting the leaves of the zucchini.

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