KR20220168723A - Health functional foods that prevent hyperlipidemia - Google Patents

Abstract

The present invention relates to a health functional food composition for hyperlipidemia having a hyperlipidemia-inhibiting effect and a method for manufacturing the same. More specifically, the present invention relates to: an autoclave extraction method that can extract the polysaccharide of Coriolus versicolor, which is a physiologically active substance, from Coriolus versicolor (fruiting body) in high yield; a health functional food composition for hyperlipidemia with novel composition which contains extract powder of Salvia miltiorrhiza, Pueraria lobata var. thomsonii, Cnidium officinale, Carthamus tinctorius seeds, and Crataegus pinnatifida to a Coriolus versicolor extraction powder containing the polysaccharide of Coriolus versicolor obtained from the same; and a method for manufacturing the same.

Description

Health functional foods that prevent hyperlipidemia}

The present invention relates to a natural composition having hyperlipidemia-inhibiting effects and a method for preparing the same. More specifically, the autoclave extraction method that can extract the polysaccharide of the mushroom, which is a physiologically active substance, from the mushroom (fruiting body) in high yield, and the mushroom extract powder containing the polysaccharide of the mushroom obtained from it. And it relates to a health functional food composition for hyperlipidemia containing hawthorn extract powder having a hyperlipidemia inhibitory effect of a new composition and a manufacturing method thereof.

Diseases such as high blood pressure, diabetes, arteriosclerosis, stroke, obesity, hyperlipidemia, and heart disease are increasing due to changes in lifestyle, especially westernization of diet, increase in fat intake and lack of exercise. This is often called an adult disease, but recently it is also called lifestyle disease.

In the past, it was recognized as a geriatric disease that commonly occurs with age, but recently, these diseases are increasingly common at a young age, and even appear in children and adolescents, so the term adult disease is no longer considered appropriate.

In particular, cardiovascular diseases account for the most common cause of death in the world as well as in the West, and their main pathogenesis is known to be atherosclerosis. High blood pressure, smoking, obesity, and hyperlipidemia are risk factors that cause atherosclerosis. It has become an important risk factor for ischemic heart disease or cerebrovascular disease, which are atherosclerotic diseases.

The oriental causes of hyperlipidemia are 1) foreign substances such as water, damp, phlegm, turbidity, and blood clots in the blood, and 2) spleen, liver, It is caused by stagnation of dampness and phlegm due to a decrease in heart function, or 3) stagnation of energy due to hwabyung (stress) and accumulation of wastes in the blood vessels. During decomposition in the liver It is known that lipotoxicity occurs and gradually accumulates in liver cells, causing fatty degeneration. Therefore, in oriental medicine, hyperlipidemia is interpreted as a disease caused by accumulation of fat and waste products in the blood vessels and liver.

Accordingly, the present inventors, as part of developing a health functional food composition containing new hyperlipidemia natural medicines, selected natural medicines used for diseases related to hyperlipidemia in oriental medicine, and improved hyperlipidemia natural medicine control (prescription composition). ) In order to construct a new composition by adding or subtracting several kinds of herbal medicine based on the control (prescription composition) recorded in the clinical as well as the classics of oriental medicine, to study the effect of the new composition on hyperlipidemia, high fat By administering the new composition to rats induced hyperlipidemia by high lipid diet mixed feed, the serum transaminase activity, GOT and GPT levels, total cholesterol, and serum triglycerides ( Triglyceride) and high-density lipoprotein cholesterol (HDL-Cholesterol) were tested, and as a result of repeated research on this, it was found that the new composition had an effect on hyperlipidemia, and the present invention was completed.

1) Fingering mushroom (fruiting body) is extracted in an autoclave extractor with one solvent selected from water, ethanol or a mixture thereof at 110-130 ° C for 2-3 hours, concentrated by filtration, and then frozen or spray-dried. Mushroom extract powder 35.0 ~ 55.0% by weight and 2) Danshen, Ginger root, cnidium, safflower seed, and mountain lion at 110 ~ 130 ℃ for 2-3 hours, respectively, separately autoclaved with one solvent selected from water, ethanol or a mixture thereof Extracted in an extractor, concentrated by filtration, and then frozen or spray-dried, 10.0-30.0% by weight of Danshen extract powder, 10.0-20.0% by weight of radish root extract powder, 1.0-10.0% by weight of cnidium extract powder, 1.0-10.0% by weight of safflower seed extract powder, A method for preparing a health functional food composition for hyperlipidemia by mixing and homogenizing 5.0 to 15.0% by weight of hawthorn extract powder and 3) one or more pharmaceutically acceptable carriers.

From the above experimental results, administration of a new composition for hyperlipidemia induced by administering a high-fat diet mixed feed resulted in total cholesterol content in serum, triglyceride content in serum, high-density lipoprotein It showed a significant lowering effect on cholesterol (HDL-Cholesterol) content. Therefore, the health functional food composition for hyperlipidemia according to the present invention is expected to have an excellent effect on improving hyperlipidemia.

Referring to the health functional food for hyperlipidemia of the present invention and its manufacturing method, the composition of the present invention contains 35.0 to 55.0% by weight of mushroom extract powder, 10.0 to 30.0% by weight of Danshen extract powder, 10.0 to 20.0% by weight of algae root extract powder, It is a health functional food composition for hyperlipidemia composed of 1.0 to 10.0% by weight of cnidium extract powder, 1.0 to 10.0% by weight of safflower seed extract powder, and 5.0 to 150% by weight of hawthorn extract powder. was extracted in an autoclave extractor with one solvent selected from water, ethanol or a mixture thereof at 110-130 ° C for 2-3 hours, concentrated by filtration, and then freeze-dried or spray-dried. % and, 2) Danshen, root, cnidium, safflower, and hawthorn were separately extracted at 110 to 130 ° C for 2-3 hours in an autoclave extractor with one solvent selected from water, ethanol, or a mixture thereof, and filtered Concentrated and then frozen or spray-dried Danshen extract powder 10.0-30.0% by weight, radish root extract powder 10.0-20.0% by weight, cnidium extract powder 1.0-10.0% by weight, safflower seed extract powder 1.0-10.0% by weight, hawthorn extract powder 5.0-15.0 % by weight and 3) a method for preparing a health functional food for hyperlipidemia by mixing and homogenizing one or more pharmaceutically acceptable carriers.

Coriolus versicolor (L.ex Fr.) Quel. is a mushroom of the Polyporaceae family. Because they come together like clouds, they are called Cloud Cloud (Cloud) and Mushroom Ji (芝), that is, Unji (Cloud 芝) in Chinese characters.

Dozens to hundreds of them grow in groups on dead trees or stumps of conifers and broad-leaved trees around the world, including Korea, and cause white rot, and are generated during the year. Mycobacteria are thin, hard leathery, semicircular, 1-5 cm wide, 1-2 mm thick, annual.

The surface is black to indigo black, with gray, tan, dark brown, dark brown, and black cloud patterns, covered with short hairs. Harvest season is summer and autumn, and dried in the sun. The taste is slightly sweet and cold. The main components are coriolan, Krestin, ATSO, and polysaccharides. Efficacy effects include dampening, anti-cancer, hepatitis B, natural hepatitis, chronic active hepatitis, chronic bronchitis, prevention of liver cancer and Treatment, digestive system cancer, breast cancer, lung cancer, anti-tumor (Sarcoma 180/mouse, inhibition rate 100%), complement activity, immune effect, cholesterol lowering, blood sugar increase suppression effect.

It is located in almost every mountain in Korea, so it is easy to find it, and after collecting and drying it, you can boil 10 to 20 grams of it in water and drink it like barley tea. It is known to have pharmacological effects while being eaten or used as herbal medicine or folk medicine. Various types of mushrooms, such as medicinal mushrooms and edible mushrooms, which are higher basidiomycetes, are growing in Korea, but research on the physiological activity as a mushroom extract is lacking. By using the autoclave extraction method as an extraction method, it was possible to extract the polysaccharide of the mushroom fingernails, which is the active ingredient of the mushroom fingernails, with higher extraction efficiency than the conventional simple reflux extraction method, It was possible to prepare a mushroom fingernail extract powder, and a new composition containing the fingernail mushroom extract powder was prepared. According to one feature of the present invention, in the method for extracting a fingernail mushroom, the extraction solvent is water, ethanol, or a mixture thereof. It can be used as one type of extraction solvent selected from the solution, and the extraction method uses the autoclave extraction method. Autoclave extraction is performed at 110 to 130 ° C. for 2 to 3 hours, preferably 2 hours. After extraction, the mixture was filtered through a 50 μm filter, concentrated to 1/10 the amount, and then 3 times the amount of ethanol was added and allowed to stand for 12 hours. After the precipitate is formed, the precipitate is collected by filtration, then dialyzed in running water for 48 hours using a Visking tube, and freeze-dried or spray-dried to prepare a mushroom extract powder.

Other herbal medicines, such as Danshen, Gale root, Cnidium, Safflower seed, and Hawthorn extract powder, are described. Danshen extract powder is a dried extract obtained by extracting the root of Salvia milltiorrhiza Bunge belonging to the Lamiaceae family (Labiatae). It contains tanshinone and cryptotanshinone, and has been used as a tonic and tonic since ancient times. It is a dried extract obtained by extracting the root, and contains daidzein and puerarin, etc., and has been used as an antiperspirant and fever reducer since ancient times.

Cnidium officinale Makino (Cnidium officinale Makino) belonging to the Apiaceae (Umbelliferae) Cnidium officinale Makino dried extract obtained by extracting the dried extract, contains cnidilide, neocnidilide, etc. It has been used for blood, tonic, and pain relief.

Safflower seed extract powder is a dried extract obtained by extracting the seeds of safflower (Carthamus tinctorius L.) belonging to the Asteraceae family (Compositae). It contains linoleic acid and has been used for the treatment and prevention of arteriosclerosis since ancient times. The extract powder is a dried extract obtained by extracting the fruits of Crataegus pinnatifida Bunge belonging to the Rosaceae family, and contains ursolic acid. has been used

According to another feature of the present invention, in the extracting means of crude drugs of a new composition except for fingernail mushrooms, one type of extraction solvent selected from water, ethanol or a mixture thereof can be used as an extraction solvent, and the extraction method is an autoclave use the extraction method.

Autoclave extraction is performed at 110 to 130 ° C. for 2 to 3 hours, preferably 2 hours. After extraction, it is filtered through a 50 μm filter, and freeze-dried or spray-dried to prepare each crude drug extract powder separately. In freeze-drying, the sample is first rapidly frozen at -30 to -50 ° C, and the frozen product is frozen at a vacuum degree of 1 to 50 ° C. The freeze-dried product obtained by treatment for 48 to 72 hours while maintaining a vacuum of about 0.1 mmHg is pulverized into a 200 mesh.

In the case of spray drying, it is treated by a known method in a room temperature type spray dryer.

The composition ratio of the mushroom extract powder constituting the health functional food composition for hyperlipidemia according to the present invention is 35.0 to 55.0% by weight, preferably 40.0 to 50.0% by weight, based on the total weight of the herbal medicine. If it is less than 35.0% by weight, it is not preferable because it does not exhibit the functionality expected for hyperlipidemia, and if it is used more than 55.0% by weight, a solidification phenomenon occurs due to the action of moisture in the filling process during capsule manufacturing, which is also undesirable.

The composition ratio of the Danshen extract powder is 10.0 to 30.0% by weight, preferably 15.0 to 25.0% by weight, based on the total weight of the crude drug. If it is less than 15.0% by weight, it is undesirable because it does not exhibit the functionality expected for hyperlipidemia, and if it is used more than 30.0% by weight, solidification occurs due to the action of moisture in the mixing process during capsule manufacturing, which is also undesirable. The composition ratio is 10.0 to 20.0% by weight, preferably 12.5 to 17.5% by weight, based on the total weight of the herbal medicine. If it is less than 10.0% by weight, it is not preferable because it does not exhibit the functionality expected for hyperlipidemia, and if it is used more than 20.0% by weight, a solidification phenomenon occurs due to the action of moisture in the granulation process during capsule manufacturing, which is also undesirable.

The composition ratio of cnidium extract powder and safflower seed extract powder is preferably 1.0 to 10.0% by weight, respectively, based on the total weight of the herbal medicine. When using more than %, a phenomenon of breaking occurs due to a decrease in bonding strength in the granulation process during capsule manufacturing, which is also undesirable.

The composition ratio of hawthorn extract powder is 5.0 to 15.0% by weight, preferably 8.0 to 12.0% by weight, based on the total weight of the crude drug. If it is less than 5.0% by weight, it is not preferable because it does not exhibit the functionality expected for hyperlipidemia, and if it is used more than 15.0% by weight, a solidification phenomenon occurs due to the action of moisture in the homogenization process during capsule manufacturing, which is also undesirable. Granules that can be formulated by mixing and homogenizing one or more pharmaceutically acceptable carriers in the composition, and containing appropriate excipients such as starch, lactose, and stevioside, stabilizers, sweeteners, pH adjusters, suspending agents, flavorings, etc. It can be taken orally in the form of tablets, capsules, pills, and liquids.

The capsules provided in the present invention follow conventional manufacturing methods. That is, the capsule of the present invention is precisely weighed and put in an autoclave extractor, extracted, filtered and freeze-dried to make a powdered mushroom extract, and separately weighs dandelion, root, cnidium, safflower seed, and mountain lion precisely, respectively. Herbal medicines are put into a separate autoclave extractor, extracted, filtered, and freeze-dried to make each extract powder. Mix, homogenize, and granulate the extract powder of the mushroom extract and each extract powder, and fill the capsules to make capsules. .

For the novel composition of the present invention prepared as described above, an acute toxicity test and an efficacy test were conducted by inducing hyperlipidemia by a high fat diet mixed feed.

As a result of the efficacy test on the new composition of the present invention, the new composition of the present invention showed a superior effect on hyperlipidemia.

It is recommended that the health functional food of the present invention be taken with water, 2 capsules (based on 450 mg of 1 capsule) at a time, 3 times a day for adults. Of course, it is possible to increase or decrease the enemy according to the condition of the user.

The present invention will be described in more detail through the following examples and experimental examples, but the present invention is not limited thereto.

Example 1: Prescription of a new composition.

Unji mushroom (fruiting body) extract powder 45.0%, Danseng extract powder 20.0%, radish root extract powder 15.0%, cnidium extract powder 5.0%, safflower seed extract powder 5.0%, hawthorn extract powder 10.0%,

Example 2: Manufacturing method of extract powder with new composition.

① Manufacture of Unji mushroom extract powder.

Cut the finger mushrooms (fruiting body) into a certain size, accurately weigh 810 g, put it in an autoclave extractor, add 10 times the amount of purified water, extract at 121 degrees Celsius for 2 hours, filter with a 50 μm filter, and reduce the amount by 1/10 After concentrating to , 3 times the amount of ethanol was added and left for 12 hours. After the precipitate is formed, the precipitate is collected by filtration, then dialyzed in running water for 48 hours using a Visking tube, and lyophilized to prepare 202.5 g of mushroom extract powder.

② Manufacture of Danshen extract powder.

322.65 g of Danshen was precisely weighed, put in an autoclave extractor, added with 10 times the amount of purified water, extracted at 121 ° C for 2 hours, filtered through a 50 μm filter, and lyophilized to prepare 90 g of Danshen extract powder.

③ Manufacture of algae root extract powder.

211.95 g of brown root was precisely weighed, put in an autoclave extractor, extracted with 10 times the amount of purified water at 121 ° C for 2 hours, filtered through a 50 μm filter, and lyophilized to prepare 22.5 g of brown root extract powder.

④ Manufacture of cnidium extract powder.

Weigh 63.45 g of cnidium precisely, put it in an autoclave extractor, add 10 times the amount of purified water, extract at 121 ° C for 2 hours, filter with a 50 μm filter, and freeze-dry to prepare 22.5 g of cnidium extract powder.

⑤ Manufacture of safflower seed extract powder.

Precisely weigh 73.8 g of safflower seeds, put them in an autoclave extractor, add 10 times the amount of purified water, extract at 121 ° C for 2 hours, filter through a 50 μm filter, and freeze-dry to prepare 22.5 g of safflower seed extract powder.

⑥ Manufacture of hawthorn extract powder.

127.35 g of hawthorn was precisely weighed, put in an autoclave extractor, and 10 times the amount of purified water was added, extracted at 121 ° C for 2 hours, filtered through a 50 μm filter, and lyophilized to prepare 45.0 g of hawthorn extract powder.

Example 3: Manufacturing method of capsules of new composition.

202.5 g of Unji mushroom (fruiting body) extract powder obtained in Example 2, 90.0 g of Danseng extract powder, 67.5 g of radish root extract powder, 22.5 g of cnidium extract powder, 22.5 g of safflower seed extract powder, and 45.0 g of hawthorn extract powder were precisely weighed and put into a blender. , Add 20 g of purified water, mix well, make granules using a granulator, make granules by drying below 60 degrees Celsius, and then use a hard capsule filling machine to fill hard capsules at 450 mg per capsule. Prepare hard capsules. Example 4: Method for preparing pills of a new composition. 202.5 g of mushroom (fruiting body) extract powder obtained in Example 2, 90.0 g of danseng extract powder, 67.5 g of radish root extract powder, 22.5 g of cnidium extract powder, 22.5 g of safflower seed extract powder, Precisely weigh 45.0 g of hawthorn extract powder, put it in a blender, add 40 g of purified water, mix well, prepare a pill using a pill maker, and then dry it below 60 degrees Celsius to make a pill. The weight of one ring is prepared as 150 mg.

Example 5: Method for preparing a liquid formulation of a new composition.

202.5 g of Unji mushroom (fruiting body) extract powder obtained in Example 2, 90.0 g of Danseng extract powder, 67.5 g of radish root extract powder, 22.5 g of cnidium extract powder, 22.5 g of safflower seed extract powder, and 45.0 g of hawthorn extract powder were precisely weighed in a mixing tank. 6000 ml of purified water and 5 g of stevioside were added, dissolved and mixed well, then added with purified water to adjust the total volume to 7000 ml, sufficiently stirred to homogenize, filtered through a 50 μm filter, and placed in a polyethylene container with a capacity of 140 ml. make a filling

Example 6: Manufacturing method of granules of new composition.

202.5 g of Unji mushroom (fruiting body) extract powder obtained in Example 2, 90.0 g of Danseng extract powder, 67.5 g of radish root extract powder, 22.5 g of cnidium extract powder, 22.5 g of safflower seed extract powder, and 45.0 g of hawthorn extract powder were precisely weighed and put into a blender. , Add 40 g of purified water, mix well, make granules using a granulator, dry below 60 degrees Celsius to make granules, and then dry below 60 degrees Celsius to prepare 450 g of granules.

Example 7: Method for manufacturing tablets of the new composition.

202.5 g of Unji mushroom (fruiting body) extract powder obtained in Example 2, 90.0 g of Danseng extract powder, 67.5 g of radish root extract powder, 22.5 g of cnidium extract powder, 22.5 g of safflower seed extract powder, and 45.0 g of hawthorn extract powder were precisely weighed and put into a blender. , Add 20 g of corn starch, 30 g of lactose, and 40 g of purified water, mix well, make granules using a granulator, dry below 60 degrees Celsius to make granules, and then dry below 60 degrees Celsius to make granules. After preparation, 4 g of magnesium stearate is added thereto and compressed into tablets directly to prepare tablets having a weight of 500 mg per tablet.

Experimental Example 1: Yield test of mushroom extract powder and mushroom polysaccharide, which is an active ingredient of mushroom mushroom (fruiting body), according to extraction conditions.

1) Manufacturing method of mushroom extract powder

① Autoclave extraction method

Cut the finger mushrooms (fruiting bodies) into a certain size, accurately weigh them, put them in an autoclave extractor, add 10 times the amount of purified water, extract them at 121 degrees Celsius for 1, 2, and 3 hours, filter them with a 50 μm filter, and After concentrating to 10%, 3 times the amount of ethanol was added and allowed to stand for 12 hours. After the precipitate is formed, the precipitate is collected by filtration, then dialyzed in running water for 48 hours using a Visking tube, and freeze-dried to prepare each fingerling mushroom extract powder.

② Hot water extraction method

Cut the Unji mushroom (fruiting body) into a certain size, accurately weigh it, put it in a reflux extractor, add 10 times the amount of purified water, extract it at 100 degrees Celsius for 3, 6, and 12 hours, filter it with a 50 μm filter to obtain 1/10 After concentrating to the same amount, 3 times the amount of ethanol is added and left for 12 hours. After the precipitate is formed, the precipitate is collected by filtration, then dialyzed in running water for 48 hours using a Visking tube, and freeze-dried to prepare each mushroom extract powder.

③ Yield calculation

Mushroom extract powder yield (w/w)% = yield of mushroom extract powder ÷ amount of mushroom extract × 100

2) Content of mushroom polysaccharide

① Experiment method

U.V. Absorbance was measured at a wavelength of 625 nm using a spectrophotometer, and the content of mushroom polysaccharide was calculated from a calibration curve prepared with glucose.

② Yield calculation

Mushroom polysaccharide yield (w/w)% = Content of Mushroom polysaccharide ÷ Amount of Mushroom Mushroom × 100

3) Experimental results (see Table 1)

The yield of the mushroom extract powder was 13.42% for 3 hours, 16.54% for 6 hours, and 18.52% for 12 hours in the hot water extraction method (100 ℃), and in the autoclave method (121 ℃, 1 atm), 1 hour 15.83%, 2 hours yielded 25.0%, and 3 hours yielded 24.8% results.

In the same extraction time period of 3 hours, the hot water extraction method (100 ℃) was 13.42%, and the autoclave method (121 ℃, 1 atm) was 24.8%.

Therefore, in the same extraction time period, the yield of the mushroom extract powder according to the autoclave method was improved by about 1.8 times. In addition, the yield of mushroom polysaccharide was 8.69% for 3 hours, 10.70% for 6 hours, and 12 The time obtained a result of 11.76%, and in the autoclave method (121 ℃, 1 atm), 10.34% for 1 hour, 16.33% for 2 hours, and 16.02% for 3 hours.

In the same extraction time period of 3 hours, the hot water extraction method (100 ℃) was 8.69%, and the autoclave method (121 ℃, 1 atmospheric pressure) obtained a result of 16.02%.

Therefore, in the same extraction time period, the yield of the mushroom polysaccharide produced by the autoclave method was improved by about 1.8 times. However, in the autoclave method (121 ℃, 1 atm), it is 25.0% at 2 hours and 24.8% at 3 hours. As a result, the yield of mushroom extract powder gradually decreases as the extraction time increases. , extraction for 2 hours was found to be more effective, and extraction for more than 2 hours did not significantly affect the yield of mushroom extract powder.

It was also found that there was no significant effect on the yield of the mushroom polysaccharide.

Therefore, comparing these experimental results, it is economical and efficient to extract in a short time for 2 hours, which is the optimal time, by the autoclave method (121 ° C, 1 atm) rather than extracting for a long time by the hot water extraction method (100 ° C). to be.

Experimental Example 4: Efficacy test of the new composition.

1) Experimental animals

Sprague-Dawley (SD) rats (weight 120∼160 g) were purchased, and after circulatory adaptation in the laboratory for about 10 days, healthy animals were selected and the temperature was 23±3 ℃, the relative humidity was 50±10%, and the exhaust was 10∼12 They were reared in an environment of 150-160 Lux, 12 hr cycle of light and shade under fluorescent lamps. Solid feed and water were sufficiently supplied during the test period.

2) Preparation of test samples

Sodium carboxymethylcellulose was added to purified water according to the dose of the capsule formulation of Example 3, and suspended at the time of use, and used as an experimental sample.

3) High-fat diet mixed feed

The high-fat diet mixture was prepared by mixing butter: cholesterol: bile juice: egg yolk: powder feed at a ratio of 3: 0.5: 0.2: 3: 9.

4) Experiment method

① Induction of hyperlipidemia by high-fat diet mixed feed

High lipid diet mixed feed was administered to female Sprague-Dawley (SD) rats for 15 days to induce hyperlipidemia, and rats with an average weight of 250 g were selected and used in the experiment.

② Sample administration

Six rats were grouped into one group, the normal group was fed powdered feed, the control group was rats induced with hyperlipidemia by administering a high-fat diet mixed feed for 15 days, and the experimental group was rats with hyperlipidemia. The mushroom extract powder (see Example 2, 1)) and the new composition (see Example 3) were orally administered at 50 mg/kg, 100 mg/kg, and 300 mg/kg per group once a day for 14 days.

③ Blood collection and serum separation

Rats were reared on a high-fat diet mixed feed, and 1 day after the final administration of the test specimen, the carotid artery was cut and blood was collected from the rats under ether anesthesia. Serum was separated by centrifugation for 10 minutes.

④ Measurement of transaminase activity in serum

The measurement of GOT and GPT activities in serum was carried out according to the Reitman-Frankel method by U.V. It was measured at a wavelength of 505 nm using a spectrophotometer.

⑤ Measurement of Total Cholesterol Content in Serum The total cholesterol content in serum was measured using a Rapid Blood Analyzer <RaBA> and cholesterol measuring kit reagents.

⑥ Measurement of triglyceride content in serum

Triglyceride content in serum was measured at a wavelength of 500 nm using a spectrophotometer and a kit reagent for measuring triglyceride.

⑦ Measurement of HDL-Cholesterol content in serum

High-density lipoprotein cholesterol (HDL-Cholesterol) content in serum was measured at a wavelength of 505 nm using a U.V.spectrophotometer according to the Enzymatic Colorimetric method.

5) Experiment results and consideration.

The results of the experiment by administering the new composition and the mushroom extract powder alone to rats raised on a high-fat diet for 15 days to induce hyperlipidemia are as follows.

① Effect of high lipid diet mixed feed alone on hyperlipidemia.

The total cholesterol content in the serum of the normal group was 47.9±6.8 mg/dl, and the total cholesterol content of the control group induced hyperlipidemia by administering the high-fat diet mixed feed alone was 141.5±13.4 At mg/dl, the total cholesterol (Total Cholesterol) content significantly increased compared to the normal group, which was sufficient to induce hyperlipidemia.

On the other hand, triglyceride content in serum was 64.5±2.9 mg/dl in the normal group and 201.7±18.3 mg/dl in the control group, and HDL-Cholesterol content was 13.9±1.8 mg/dl in the normal group. dl, the control group also showed a significant increase to 25.3±2.1mg/dl.

② Effects of single administration of gungji mushroom extract powder on hyperlipidemia-induced rats.

Compared to the control group administered with the high-fat diet mixed feed alone, the test group administered with mushroom extract powder generally showed a decrease in GOT and GPT values in a dose-dependent manner. Mushroom extract powder 50 mg/kg, 100 As a result of the test after administering mg/kg and 300 mg/kg once a day, respectively, the GOT values of these test groups were 175.8±13.4, 167.3±12.1, and 134.5±11.9, respectively, compared to the GOT value of 387.4±21.6 in the control group. The GPT values of these test groups were 88.7±3.5, 86.4±4.2, and 80.6±5.8, respectively, showing a significant drop in GPT value in a dose-dependent manner compared to the GPT value of 249.3±25.4 in the control group. The total cholesterol (Total Cholesterol) content in the serum of these test groups was 81.2±3.4 mg/dl, 78.5±4.6 mg/dl, and 72.4±3.9 mg/dl, respectively. In the group administered with and 300 mg/kg, a significant result was shown in a dose-dependent manner compared to the total cholesterol content of 141.5±13.4 mg/dl in the control group administered with the high lipid diet mixed feed alone.

On the other hand, triglyceride content in the serum of these test groups was 56.2±5.9 mg/dl, 54.7±4.1 mg/dl, and 49.8±5.6 mg/dl, respectively. Compared to the serum triglyceride content of the control group, 201.7 ± 18.3 mg / dl, all showed significant results, and the result was rather lower than the triglyceride content of the normal group, 64.5 ± 2.9 mg / dl In addition, the high-density lipoprotein cholesterol (HDL-Cholesterol) content was 25.3±2.1 mg/dl in the control group, and 11.4±1.9 mg/dl, 12.7±1.2 mg/dl and 14.5±1.5 mg/dl in these test groups, respectively. dl showed a significant synergistic inhibitory effect when compared to the control group.

③ Effect of administration of the new composition according to the present invention on hyperlipidemia-induced rats.

A) Transaminase activity in serum ⅰ) Effect on GOT and GPT activity Looking at the effect, the normal group was 119.5±13.2, and the hyperlipidemia-induced control group rose to 387.4±21.6. Compared to the normal group, the GOT level in the blood significantly increased, which was sufficient to induce hyperlipidemia.

ii) Total Cholesterol, Triglyceride, and HDL-Cholesterol in Serum.

Compared to the control group administered with only the high-fat diet mixed feed, in general, the test group administered with the new composition exhibited significant suppression of hyperlipidemia in a dose-dependent manner.

50 mg/kg, 100 mg/kg, and 300 mg/kg of the new composition according to the present invention were administered and tested once a day, respectively. As a result, the total cholesterol content in the serum of these test groups was 75.7 ± 6.3, respectively. mg/dl, 71.4±5.8 mg/dl and 52.9±3.5 mg/dl, dose-dependent compared to the total cholesterol content of 141.5±13.4 mg/dl in the control group administered only with the high-fat diet mixed feed alone Meanwhile, the serum triglyceride content of these test groups was 53.5 ± 4.2 mg/dl, 49.3 ± 3.5 mg/dl, and 48.9 ± 5.8 mg/dl, respectively. ) Compared to the triglyceride content of 201.7 ± 18.3 mg / dl of the control group in which only the mixed feed was poisoned, all significant results were shown, and the new composition according to the present invention (Example 3) 100 mg / kg and 300 mg / kg The triglyceride content by kg administration was 49.3 ± 3.5 mg/dl and 48.9 ± 5.8 mg/dl, which showed a significant decrease compared to the normal group's triglyceride content of 64.5 ± 2.9 mg/dl. .

Furthermore, looking at the effect on the content of high-density lipoprotein cholesterol (HDL-cholesterol) in serum, compared to the control group administered only the high-fat diet mixed feed alone,

The high-density lipoprotein cholesterol (HDLcholesterol) content by administration of 50 mg/kg, 100 mg/kg and 300 mg/kg of the new composition of the present invention was 11.6±1.7 mg/dl, 12.8±1.3 mg/dl and 19.3±2.6 mg/dl. As dl, the high-density lipoprotein-cholesterol (HDL-cholesterol) content of the control group administered only with the high-fat diet mixed feed was 25.3±2.1 mg/dl.

Claims (1)

1) Fingering mushroom (fruiting body) is extracted in an autoclave extractor with one solvent selected from water, ethanol or a mixture thereof at 110-130 ° C for 2-3 hours, concentrated by filtration, and then frozen or spray-dried. Mushroom extract powder 35.0 ~ 55.0% by weight and 2) Danshen, Ginger root, cnidium, safflower seed, and mountain lion at 110 ~ 130 ℃ for 2-3 hours, respectively, separately autoclaved with one solvent selected from water, ethanol or a mixture thereof Extracted in an extractor, concentrated by filtration, and then frozen or spray-dried, 10.0-30.0% by weight of Danshen extract powder, 10.0-20.0% by weight of radish root extract powder, 1.0-10.0% by weight of cnidium extract powder, 1.0-10.0% by weight of safflower seed extract powder, A method for preparing a health functional food composition for hyperlipidemia by mixing and homogenizing 5.0 to 15.0% by weight of hawthorn extract powder and 3) one or more pharmaceutically acceptable carriers.