KR20220152553A - 스테로이드의 히드록실화 방법 - Google Patents

스테로이드의 히드록실화 방법 Download PDF

Info

Publication number
KR20220152553A
KR20220152553A KR1020227034689A KR20227034689A KR20220152553A KR 20220152553 A KR20220152553 A KR 20220152553A KR 1020227034689 A KR1020227034689 A KR 1020227034689A KR 20227034689 A KR20227034689 A KR 20227034689A KR 20220152553 A KR20220152553 A KR 20220152553A
Authority
KR
South Korea
Prior art keywords
acid
cholane
dehydrogenase
group
dihydroxy
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020227034689A
Other languages
English (en)
Korean (ko)
Inventor
니콜 스타우니그
카이 올리버 돈스바흐
Original Assignee
안니키 게엠베하
파마젤 게엠베하
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 안니키 게엠베하, 파마젤 게엠베하 filed Critical 안니키 게엠베하
Publication of KR20220152553A publication Critical patent/KR20220152553A/ko
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/14—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen (1.14.14)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Steroid Compounds (AREA)
KR1020227034689A 2020-03-06 2021-03-05 스테로이드의 히드록실화 방법 Pending KR20220152553A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20161537.4 2020-03-06
EP20161537.4A EP3875597A1 (de) 2020-03-06 2020-03-06 Verfahren zur hydroxylierung von steroiden
PCT/EP2021/055615 WO2021176066A1 (de) 2020-03-06 2021-03-05 Verfahren zur hydroxylierung von steroiden

Publications (1)

Publication Number Publication Date
KR20220152553A true KR20220152553A (ko) 2022-11-16

Family

ID=69780053

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020227034689A Pending KR20220152553A (ko) 2020-03-06 2021-03-05 스테로이드의 히드록실화 방법

Country Status (5)

Country Link
US (1) US12601000B2 (https=)
EP (2) EP3875597A1 (https=)
JP (1) JP7851562B2 (https=)
KR (1) KR20220152553A (https=)
WO (1) WO2021176066A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117025709A (zh) * 2023-07-31 2023-11-10 华南理工大学 一种细胞色素p450酶联合细胞色素p450还原酶在合成熊去氧胆酸中的应用
NL2035768B1 (en) * 2023-09-08 2024-07-04 Centrient Pharmaceuticals Netherlands B V Method for hydroxylation of bile acids or analogues thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102002518B (zh) * 2010-10-28 2015-05-13 浙江大学 一种酶羟化3β-胆固醇乙酸酯制备7β-羟基-3β胆固醇乙酸酯的方法
CN109154016B (zh) * 2017-12-29 2021-11-16 邦泰生物工程(深圳)有限公司 一种化学-酶法制备熊去氧胆酸的方法
GB201819209D0 (en) * 2018-11-26 2019-01-09 Hypha Discovery Ltd Biocatalytic techniques
EP3875596A1 (de) 2020-03-06 2021-09-08 Pharmazell GmbH Verfahren zur hydroxylierung von steroiden

Also Published As

Publication number Publication date
US20240209410A1 (en) 2024-06-27
EP3875597A1 (de) 2021-09-08
WO2021176066A1 (de) 2021-09-10
JP7851562B2 (ja) 2026-04-27
EP4114961A1 (de) 2023-01-11
US12601000B2 (en) 2026-04-14
JP2023516682A (ja) 2023-04-20

Similar Documents

Publication Publication Date Title
CN103097400B (zh) 新的7α-羟类固醇脱氢酶敲除突变体及其用途
Yao et al. Identification and engineering of cholesterol oxidases involved in the initial step of sterols catabolism in Mycobacterium neoaurum
CN102007210B (zh) 12α-羟类固醇脱氢酶，产生及其用途
Zhang et al. Bioconversion of 4-androstene-3, 17-dione to androst-1, 4-diene-3, 17-dione by recombinant Bacillus subtilis expressing ksdd gene encoding 3-ketosteroid-Δ1-dehydrogenase from Mycobacterium neoaurum JC-12
JP5627546B2 (ja) セコジオン誘導体のエナンチオ選択的な酵素的還元の方法
CN112457412B (zh) 一种人工电子传递系统及其在促进p450酶羟基化反应中的应用
JP2016521544A (ja) デヒドロエピアンドロステロンおよびその中間体の調製法。
US12601000B2 (en) Method for the hydroxylation of steroids
EP2130907B1 (en) Transformed strain derived from strain deficient in multidrug efflux protein, and bioconversion method using the same
de Las Heras et al. Cholesterol to cholestenone oxidation by ChoG, the main extracellular cholesterol oxidase of Rhodococcus ruber strain Chol-4
Karpov et al. Creation and functional analysis of Mycolicibacterium smegmatis recombinant strains carrying the bacillary cytochromes CYP106A1 and CYP106A2 genes
Guevara et al. Functional characterization of 3-ketosteroid 9α-hydroxylases in Rhodococcus ruber strain chol-4
WO2025044960A1 (zh) 一种酮还原酶突变体及其应用
CN115725522B (zh) 甾体激素脱氢酶、基因、突变体、构建方法和应用
WO2007138894A1 (ja) 水酸化酵素遺伝子及びその用途
Karpov et al. Pregnenolone and progesterone production from natural sterols using recombinant strain of Mycolicibacterium smegmatis mc2 155 expressing mammalian steroidogenesis system
JP7783187B2 (ja) ステロイドのヒドロキシ化方法
Wiens et al. Biosynthesis of 1α‐hydroxycorticosterone in the winter skate Leucoraja ocellata: evidence to suggest a novel steroidogenic route
CN110835639B (zh) 一种制备(s)-1,2,3,4-四氢异喹啉-1-甲酸及其衍生物的方法
Venu Gopal et al. Production of 17-keto androstene steroids by the side chain cleavage of progesterone with Bacillus sphaericus
US20240263208A1 (en) Cytochrome p450 enzyme
CN117015612A (zh) 还原型辅酶q10的生产工艺
CN119120404A (zh) 羰基还原酶突变体及其在制备(2r,3r)-2-氟-3-羟基酯类化合物中的应用
WO2024192335A1 (en) Alternative strategies for methanobactin production
CN117025709A (zh) 一种细胞色素p450酶联合细胞色素p450还原酶在合成熊去氧胆酸中的应用

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

A201 Request for examination
E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

D21 Rejection of application intended

Free format text: ST27 STATUS EVENT CODE: A-1-2-D10-D21-EXM-PE0902 (AS PROVIDED BY THE NATIONAL OFFICE)

PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-NAP-X000 (AS PROVIDED BY THE NATIONAL OFFICE)

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000