KR20220127578A - Bioconverted plant material cosmetic composition and use thereof - Google Patents

Abstract

The present invention relates to a method for preparing a cosmetic composition comprising a plant material composition having been subjected to a biotransformation process, and to a cosmetic composition having antibacterial and antioxidant effects using the same. The cosmetic composition according to the present invention, which is prepared by allowing natural material extracts to be subjected to a biotransformation process, is not only harmless to the human body but also has excellent antibacterial and antioxidant effects, so that it can be effectively used in various types of cosmetic compositions including shampoo, shower gel, and hair essence.

Description

Bioconverted plant material cosmetic composition and use thereof

The present invention relates to a method for manufacturing a cosmetic comprising a bioconverted plant material composition, and a cosmetic composition for antibacterial and antioxidant using the same.

Recently, the use of natural extracts is increasing in the cosmetic field, and after they have been reported to be excellent in various aspects such as moisturizing power and anti-aging function, many studies have been made on cosmetic compositions using herbal extracts. However, these natural herbal extracts are very rarely put to practical use due to their insignificant cosmetic activity such as antioxidant and skin elasticity enhancement. Further development is required.

A number of conventional natural cosmetic compositions have been proposed. Among them, Korean Patent Laid-Open No. 10-2013-0124724 proposes a composition for preventing skin aging comprising an extract of Ixeris dentata as an active ingredient. In this case, the plant extract increases intracellular ceramide synthesis, and in particular, the ethyl acetate fraction of the extract increases ceramide synthesis with excellent efficiency compared to other fractions, and thus, the plantar genus extract increases the treatment, improvement or prevention of skin diseases such as atopic dermatitis. It has been reported to have anti-aging and skin moisturizing effects.

In addition, Korean Patent Application Laid-Open No. 10-2004-0104000 proposes a cosmetic composition for alleviating skin irritation containing an extract of sagebrush and Gomari stabilized with nanoliposomes, and provides a cosmetic composition with improved precipitation and discoloration and excellent irritation relief effect. It is stated that

In addition, in Korea Patent Registration No. 10-1405429, in the cosmetic composition containing wild herbs, the wild herbs include sauerkraut, horseradish, horsetail, chrysanthemum, juniper, chrysanthemum, leathery herbs, porcupine, celandine, celandine, snake strawberry, sedum, sedum dung, sagebrush, marigold, chrysanthemum grass, amaranth, ginseng, ginseng, shamrock, red ginseng vine, gourd sagebrush, Eosongcho, wild stone bean, blood, arrowroot vine, black locust, foxtail, American ginseng , Sagebrush, Potato Potato, Seaweed odor, Artemisia sagebrush, Gingerbread, Coltsfoot, Mosquito, Mugwort, Thistle, Dandelion, Syringa, sagebrush, Bibichu, Wonchuri, algae, wheat herbs, Wheatgrass, Maple odor, Caesarnia spp. Brassica sagebrush, jangguchae, bindweed, yarrow, ragweed, violet, jasmine, purslane, lamifolia, chrysanthemum, green fern, oak greens, baby swimming, chichi, sagebrush, valerian, body herbs, brisket, plantain, sandalwood, We propose a wild herb cosmetic, characterized in that it is any one or two or more selected from the group consisting of spiny berries, peppermint, chazugi, gourdae, ginseng chrysanthemum, giraffe choi, mulberry, baechohyang, banyan grass, poison ivy, sagebrush, cheongung, and chicory.

However, in the case of these wild herb cosmetics, the selective properties of specific ingredients are not considered at all, and it is vaguely limited to the extent to which several wild plants are applied to cosmetics, so more research on natural product-based cosmetic compositions is required.

Republic of Korea Patent Publication No. 10-1405429

It is an object of the present invention to provide a method for producing a bioconverted plant material composition.

Another object of the present invention is to provide a cosmetic composition prepared by the above method.

In order to achieve the object of the present invention, the present invention provides a) a mixture containing moringa leaves, calamus, goji berry, pomegranate, centella asiatica, rosemary and lemon with citric acid, ascorbic acid and tyrosine. ) extracting with a first solvent containing and a second solvent containing ethanol (Ethanol) and hydrochloric acid (HCl), respectively; and

b) provides a method for preparing a cosmetic composition, comprising the step of fermenting by mixing the extract extracted with the first solvent and the extract extracted with the second solvent in step a).

In one embodiment of the present invention, the mixture of step a) is 25 to 35 parts by weight of moringa leaves, 15 to 25 parts by weight of iris, 5 to 15 parts by weight of wolfberry, 5 to 15 parts by weight of pomegranate based on 100 parts by weight of the mixture. , 5 to 15 parts by weight of Centella asiatica, 5 to 15 parts by weight of rosemary, and 5 to 15 parts by weight of lemon.

In one embodiment of the present invention, the fermentation of step b) may be performed using yeast (Saccharomyces cerevisiae sp.).

In addition, the present invention provides a cosmetic composition prepared by the above method.

In one embodiment of the present invention, the composition may be used for antibacterial and antioxidant.

In one embodiment of the present invention, the cosmetics include soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, body wash, shower gel, skin lotion, skin softener, skin toner, lotion, milk lotion, It may be selected from the group consisting of moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, essence, nourishing essence, pack, emulsion, lipstick, makeup base, foundation, press powder, loose powder and eye shadow.

The cosmetic composition of the present invention is made by bioconversion of a natural material extract, and is not only harmless to the human body, but also has excellent antibacterial and antioxidant effects, so it can be usefully used in various cosmetic fields including shampoo, shower gel, and hair essence.

Figure 1 confirms the antibacterial activity of the composition of the present invention.
Figure 2 confirms the antioxidant effect of the composition of the present invention.
Figure 3 shows the microbiological test results of the shampoo and shower gel containing the composition of the present invention.
Figure 4 confirms whether the heavy metal is detected in the shampoo containing the composition of the present invention.
5 is a view confirming whether heavy metals are detected in the shower gel including the composition of the present invention.
6 shows the results of the eye mucous membrane test of the shampoo containing the composition of the present invention.
7 shows the results of the eye mucous membrane test of the shower gel containing the composition of the present invention.

Hereinafter, the present invention will be described in detail.

The present invention provides a) a mixture containing moringa leaf, calamus, goji berry, pomegranate, centella asiatica, rosemary and lemon in a first solvent containing citric acid, ascorbic acid and tyrosine and ethanol (Ethanol) and hydrochloric acid (HCl) including extraction with each of the second solvent; and

b) provides a method for preparing a cosmetic composition, comprising the step of fermenting by mixing the extract extracted with the first solvent and the extract extracted with the second solvent in step a).

Moringa, calamus, goji berry, pomegranate, centella asiatica, rosemary and lemon used in the present invention are whole plants or parts of plants such as roots, stems, leaves, fruits, branches, peels, etc. Preferably, moringa leaves may be used, goji berries, pomegranates and lemons may be used, calamus and Centella asiatica may be used, and rosemary stems and leaves may be used.

In the present invention, the mixture may include moringa leaf, calamus, goji berry, pomegranate, centella asiatica, rosemary and lemon, and the plant material may be pulverized before or after mixing.

In the present invention, the plant material may be dried.

The first solvent may include citric acid, ascorbic acid and tyrosine, preferably 0.01 to 0.1 mM citric acid, 1 to 3 mM ascorbic acid, and 10 to 30 uM tyrosine. may contain, and more preferably, may contain 0.05 mM citric acid, 2 mM ascorbic acid, and 20 uM tyrosine. The citric acid, ascorbic acid and tyrosine may be dissolved in one or more substances selected from the group consisting of water, C ₁ to C ₄ alcohol, hexane, and mixtures thereof.

The second solvent may include ethanol (Ethanol) and hydrochloric acid (HCl), preferably 60 to 80% ethanol and 0.005 to 0.015N hydrochloric acid, more preferably 70% ethanol and 0.01N hydrochloric acid.

In the present invention, the extract may mean a result such as a liquid component obtained by immersing the extraction raw material in a solvent and then extracting for a certain period of time at a low temperature, room temperature or warm state, a solid obtained by removing the solvent from the liquid component, etc. It is not limited thereto, and may include an extract, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, or all of these crude products or purified products.

In the present invention, the extract extracted with the first solvent and the extract extracted with the second solvent may be mixed in a ratio of 1: 0.5 to 2, preferably in a ratio of 1:1.

In the present invention, fermentation may be carried out at 35 to 45° C. for 2 to 5 days, and more preferably, it may be fermented for 2 days.

In the present invention, the mixture of step a) is 25 to 35 parts by weight of moringa leaves, 15 to 25 parts by weight of calamus, 5 to 15 parts by weight of goji fruit, 5 to 15 parts by weight of pomegranate, 5 to 15 parts by weight of centella based on 100 parts by weight of the mixture. 15 parts by weight, 5 to 15 parts by weight of rosemary, and 5 to 15 parts by weight of lemon, preferably, the mixture is based on 100 parts by weight of the mixture, based on 100 parts by weight of the mixture, moringa leaves 30 parts by weight, irises 20 parts by weight, goji berries 10 parts by weight , 10 parts by weight of pomegranate, 10 parts by weight of Centella asiatica, 10 parts by weight of rosemary and 10 parts by weight of lemon.

In the present invention, the fermentation of step b) may use yeast (Saccharomyces cerevisiae sp.).

In another aspect, the present invention provides a cosmetic composition prepared by the method.

The cosmetic composition of the present invention may include not only the extract fermented product of the present invention, but also ingredients commonly used in the cosmetic composition, for example, conventional antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances. adjuvants, and carriers.

In addition, the composition of the present invention may also be used by mixing the organic sunscreen that has been conventionally used as long as it does not damage the skin protection effect by reacting with the extract fermented product of the present invention.

Products to which the cosmetic composition of the present invention can be added include, for example, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, body wash, shower gel, skin lotion, skin softener, and skin. Toner, Lotion, Milk Lotion, Moisture Lotion, Nourishing Lotion, Massage Cream, Nourishing Cream, Moisture Cream, Hand Cream, Essence, Nourishing Essence, Pack, Emulsion, Lipstick, Makeup Base, Foundation, Press Powder, Loose Powder, Eye Shadow, etc. It may include a formulation of, those skilled in the art can prepare based on common knowledge in the art by containing the components of the extraction mixture of the present invention.

The composition of the present invention may have antibacterial and antioxidant uses. In Example 3 of the present invention, it was confirmed that the composition of the present invention had excellent antibacterial activity, and in Example 4, it was confirmed that the composition of the present invention had excellent antioxidant activity.

Hereinafter, preferred examples are presented to help the understanding of the present invention. However, the following examples are only provided for easier understanding of the present invention, and the contents of the present invention are not limited by the following examples.

Example

<Example 1: Preparation of Materials>

Moringa was purchased from a domestic agricultural company, and goji berries, iris, pomegranate, centella asiatica, rosemary and lemon were purchased from an online mall that sells oriental medicine ingredients. Moringa leaves were used, Goji berries, pomegranates and lemons used fruits, irises and Centella asiatica were used, and rosemary stems and leaves were used. All of the plant materials were dried. In addition, reagents and materials used in the present invention were all commercially available.

<Example 2: Preparation of fermented natural extract>

Moringa leaves 300 g, calamus 200 g, goji berry 100 g, pomegranate 100 g, centella asiatica 100 g, rosemary stems and leaves 100 g, and lemon 100 g were crushed and mixed and then quantified (30 g/dry wt./L).

A first solvent (EB01, 0.05 mM Citric acid, 2 mM ascorbic acid, distilled water containing 20 μM tyrosine) and a second solvent (EB02, 70% acidic Ethanol, 0.01N HCL) were prepared, and then the pulverized product was mixed with each solvent. Extracted (the first solvent and the second solvent were extracted using half of the pulverized product).

The first solvent was adjusted to be 1.5 times the weight of the pulverized product, extracted at high pressure and low temperature (1.2 Psi, 50° C.) for 3 hours, and then filtered through 4 layers of gauze to prepare an extract, and the filtrate was freeze-dried and used as a material was utilized.

The second solvent was adjusted to 1.5 times the weight of the pulverized product, and then extracted by shaking (50° C., 130 rpm) for 12 hours, followed by filtration with 4 layers of gauze. Then, the ethanol was evaporated by concentration under the conditions of low temperature and high pressure (1.2 Psi, 50 ℃). The residue was removed by centrifugation (8,000 rpm/15 min, 4 h) and the supernatant was taken. Then, fractionation (using Filter paper #3) was performed, and the fraction was freeze-dried and used as a material.

The extract using the first solvent and the extract using the second solvent were mixed in equal amounts and the yeast strain (Saccharomyces cerevisiae sp.) was used at about 35° C. for 1, 2, 3, 4 and 5 days, respectively. Conversion to prepare a natural extract fermented product of the present invention.

<Example 3: Antimicrobial Activity Assay>

Whether or not antibacterial activity against the fermented product prepared in Example 2 was confirmed using P.acne.

For the measurement of antimicrobial activity, the dried composition of the present invention was suspended in physiological saline, the test bacterial solution was added, mixed, and left at room temperature for 5 minutes, and the antimicrobial activity according to the change of time was measured in an incubator. The test solution was diluted step-by-step, and 1 mL was dispensed into 2 Petri dishes for each concentration, and (15-25) mL of prepared agar at (45-50)°C was dispensed into the Petri dishes, and coagulated at room temperature. The solidified Petri dish was inverted and cultured at (35 ± 1) °C for (24 ~ 48) hours to observe the number of viable cells. The number of viable cells was calculated according to (Equation 1), and the sterilization reduction rate was determined according to (Equation 2).

[Equation 1] Calculation of viable cells

N = C × D

N: number of viable cells, C: number of colonies (average of the number of colonies of 2 sheets), D: dilution factor

[Equation 2] Calculation of antibacterial effect (%)

R(%) = [(A - B) / A] × 100

R: antibacterial effect, A: the initial number of viable cells in the control group (average),

B: Number of viable cells after a certain period of time in the test group (average)

As a result, as shown in FIG. 1 , the antimicrobial activity showed significant growth inhibition after 5 days at a concentration of 2%, and the effect according to the incubation time was somewhat higher at 48 hours.

<Example 4: Antioxidant Activity Assay>

Antioxidative effect was measured by using DPPH (2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl) to measure the antioxidant effect by reducing power using free radical free radicals.

The DPPH test method is a representative experiment to confirm the scavenging ability of free radicals. 1 mL of ethanol is added to 1 mL of 0.2 mM DPPH solution dissolved in methanol, 1 mL of fermented product (extract = sample) is added and mixed, and then mixed at room temperature for 10 minutes. After standing for a minute, the absorbance was measured at 517 nm using a spectrophotometer. Antioxidative activity was measured by the following formula for the inhibition of the scavenging activity of DPPH, using the case without the sample as the control and the case with the sample as the experiment.

[Equation 3]

Inhibition (%) = {1 - [(AExperiment - ABlank)/ AControl]} × 100

As a result, as shown in FIG. 2, as the concentration of the yeast fermented product of Moringa extract increased, the free radical scavenging ability increased, and it was confirmed that it was about 75% of the 25μg/ml vitamin C used as a standard material at 4% concentration.

<Example 5: Preparation of products using fermented natural extracts>

In order to confirm the suitable formulation of three types of body such as shampoo/shower gel/hair essence using natural materials, our basic formulation was slightly modified and each composition was mixed as follows to prepare a formulation.

Ingredients (Shampoo Composition) Weight ratio (%) Cosmetic composition of the present invention 8.0 Hydrolyzed Keratin 1.5 Hydrolyzed Collagen 1.5 dexpanthenol 1.2 L-menthol 0.3 Disodium Laureth Sulfosuccinate 0.8 Decylglucoside 0.1 Cocamidopropyl Betaine 0.2 Disodium EDIT 0.15 PEG-120 Methylglucose Dioleate 0.25 Polyquaternium-10 0.1 1,2-Hexanediol 1.0 Citric Acid 0.8 Glyceryl Caprylate 3.0 Salicylic Acid 1.0 Spices 0.1 Purified water balance Sum 100

Ingredients (shower gel) Weight ratio (%) Cosmetic composition of the present invention 9.0 lauryl diethanolamide 6.0 Sodium polyoxyethylene lauryl ether sulfate solution 30 Sodium Lauryl Sulfate Solution 15 propylene glycol 1.0 Methylparahydroxybenzoate 0.2 Spices 0.5 citric acid 1.3 Purified water balance Sum 100

Ingredients (hair essence) Weight ratio (%) Cosmetic composition of the present invention 10 Polysorbate 60 0.5 Trehalose 0.8 dexpanthenol 0.1 Carbomer 0.15 xanthan gum 1.5 Hydrolyzed Keratin 2.0 hydrolyzed elastin 1.5 Hydrolyzed Collagen 1.5 Disodium EDIT 0.15 Glyceryl Caprylate 0.1 Salicylic Acid 0.5 Arginine 0.8 L-menthol 0.3 Sodium Hyraluronate 1.5 Spices 0.1 Purified water balance Sum 100

In order to confirm the safety of the finished formulation and composition, two types of shampoo and shower gel were commissioned to a specialized clinical laboratory and tested for microorganisms, heavy metals and eye mucosa. Both microorganisms and heavy metal test results through clinical trials were judged as “non-detection” ( FIGS. 3 to 5 ).

No bleeding or thawing or coagulation reactions were observed in the ocular mucosal stability test. Therefore, for both shampoo and shower gel, the stimulation index (S score) for 6 fertilized eggs was calculated as '16' for the stock solution, which corresponds to 'Severely irritating', and for the 10% concentration, it was calculated as '15' to 'Irritating'. ', 1% concentration was calculated as '3', confirming that it corresponds to 'Nonirritant or Slightly irritating'.

In the case of the negative control group, the stimulation index (S score) for 6 fertilized eggs was '0', which was confirmed to correspond to 'Nonirritant or Slightly irritating'. In the case of the positive control group, the stimulation index (S score) for 6 fertilized eggs is calculated as '15', confirming that it corresponds to 'Irritating'. From the above results, when evaluating eye mucous membrane irritation for shampoo and shower gel using the HET-CAM test method, the undiluted solution corresponds to 'Severely irritating', 10% concentration is 'Irritating', and 1% concentration corresponds to 'Nonirritant or Slightly irritating'. It was confirmed that it was considered as a material ( FIGS. 6 and 7 ).

Claims (6)

a) a mixture containing moringa leaf, calamus, goji berry, pomegranate, centella asiatica, rosemary and lemon in a first solvent containing citric acid, ascorbic acid and tyrosine and ethanol and extracting with each of a second solvent including hydrochloric acid (HCl); and
b) mixing the extract extracted with the first solvent and the extract extracted with the second solvent in step a) and fermenting, a method for preparing a cosmetic composition.
The method according to claim 1, wherein the mixture of step a) is based on 100 parts by weight of the mixture, based on 100 parts by weight of the mixture, Moringa leaves 25 to 35 parts by weight, Calamus 15 to 25 parts by weight, Goji berry 5 to 15 parts by weight, Pomegranate 5 to 15 parts by weight, Centella asiatica 5 to 15 parts by weight, 5 to 15 parts by weight of rosemary, and 5 to 15 parts by weight of lemon, the method for preparing a cosmetic composition.
The method according to claim 1, wherein the fermentation in step b) uses yeast (Saccharomyces cerevisiae sp.).
A cosmetic composition prepared by the method of any one of claims 1 to 3.
The cosmetic composition according to claim 4, wherein the composition is for antibacterial and antioxidant use.
According to claim 4, wherein the cosmetic is soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, body wash, shower gel, skin lotion, skin softener, skin toner, lotion, milk lotion, and moisture lotion. , nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, essence, nourishing essence, pack, emulsion, lipstick, makeup base, foundation, press powder, loose powder and eye shadow, which is selected from the group consisting of, cosmetic composition.

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