KR102642480B1 - Bioconverted plant material cosmetic composition and use thereof - Google Patents

Abstract

The present invention relates to a method for producing a cosmetic containing a bioconverted plant material composition and an antibacterial and antioxidant cosmetic composition using the same. Since the cosmetic composition is manufactured by bioconverting a natural material extract, it is not only harmless to the human body but also has an antibacterial effect. And because it has excellent antioxidant effects, it can be usefully used in various cosmetic fields including shampoo, shower gel, and hair essence.

Description

Bioconverted plant material cosmetic composition and use thereof}

The present invention relates to a method for producing a cosmetic containing a bioconverted plant material composition and an antibacterial and antioxidant cosmetic composition using the same.

Recently, the use of natural extracts has been increasing in the cosmetics field, and after they were reported to be excellent in various aspects such as moisturizing ability and anti-aging function, much research is being conducted on cosmetic compositions using herbal extracts. However, these natural herbal extracts have very little activity as cosmetics, such as antioxidant properties and increasing skin elasticity, so they are rarely put to practical use. Therefore, new natural cosmetics that are stable without irritating the skin and have excellent skin moisturizing, skin elasticity, and skin regeneration effects are being developed. More development is required.

Many conventional natural cosmetic compositions have been proposed, and among them, Korean Patent Publication No. 10-2013-0124724 proposes a composition for preventing skin aging containing Ixeris dentata extract as an active ingredient. Here, the extract of Siberia chinensis increases intracellular ceramide synthesis, and in particular, the ethyl acetate fraction of the extract increases ceramide synthesis with superior efficiency compared to other fractions, and because of this, the extract of Siberia rhododendron increases the synthesis of ceramides in cells, and as a result, the extract of Siberian ceramide increases ceramide synthesis in cells, and in particular, the ethyl acetate fraction of the extract increases ceramide synthesis with superior efficiency compared to other fractions. As a result, the extract of Siberian asterium increases the synthesis of ceramides in cells. It is effective and has been reported to have anti-aging and skin moisturizing effects.

In addition, Korean Patent Publication No. 10-2004-0104000 proposes a cosmetic composition for relieving skin irritation containing extracts of Siberian sage and Cormica sagebrush stabilized with nanoliposomes, providing a cosmetic composition that improves precipitation and discoloration and has an excellent irritation-relieving effect. It is stated that it does.

In addition, in Korean Patent Registration No. 10-1405429, in the cosmetic composition containing wild herbs, the wild herbs include coriander, shepherd's purse, horsetail, eucalyptus, chickweed, chickweed, leathern herb, prickly pear, herbaceous plant, celandine, snake strawberry, Sedum, sedum, sedge, pigweed, chicken's lanceolate, amaranthus, sagebrush, ginseng, clover, ginseng vine, pumpkin's lanceolate, hairy chickweed, Houttuynia cordata, wild soybean, blood, kudzu vine, blackcurrant, foxtail, foxglove, syringa. , Japanese daisy, pig's potato, seaweed, mugwort, ant snail, butterbur, bear's ragweed, mugwort, thistle, dandelion, sorghum, bitter gourd, day lily, daylily, millet, wheat sprouts, grass floss, maple sedge, sea basil, perilla seedlings, dandelion root, Gomari, hairy lily, columbine, bindweed, yarrow, coriander, violet, warbler's knee, purslane, honey plant, bow sprouts, sage greens, sesame greens, baby's swim, borage, moongave, valerian, body herb, prickly pear, plantain, paramecium, We are proposing wild herb cosmetics, characterized in that they are one or two or more types selected from the group consisting of Spine herb, peppermint, chazugi, cypress, bleeding heart, giraffe herb, rhizome, pear herb, herbaceous plant, solitary herb, lily, lily, and chicory.

However, in the case of these wild herb cosmetics, the selective characteristics of specific ingredients are not considered at all and are limited to vaguely applying various wild herbs to cosmetics, so more research on natural product-based cosmetic compositions is required.

Republic of Korea Patent Publication No. 10-1405429

The purpose of the present invention is to provide a method for producing a bioconverted plant material composition.

Another object of the present invention is to provide a cosmetic composition prepared by the above method.

In order to achieve the object of the present invention, a) a mixture containing moringa leaves, calamus, goji berry, pomegranate, centella asiatica, rosemary and lemon is mixed with citric acid, ascorbic acid and tyrosine. Extracting with a first solvent containing ) and a second solvent containing ethanol and hydrochloric acid (HCl); and

It provides a method for producing a cosmetic composition, including the step of b) mixing and fermenting the extract extracted with the first solvent and the extract extracted with the second solvent in step a).

In one embodiment of the present invention, the mixture in step a) includes 25 to 35 parts by weight of moringa leaves, 15 to 25 parts by weight of iris, 5 to 15 parts by weight of goji berry, and 5 to 15 parts by weight of pomegranate, based on 100 parts by weight of the mixture. , 5 to 15 parts by weight of centella asiatica, 5 to 15 parts by weight of rosemary, and 5 to 15 parts by weight of lemon.

In one embodiment of the present invention, the fermentation in step b) may be performed using yeast (Saccharomyces cerevisiae sp.).

Additionally, the present invention provides a cosmetic composition prepared by the above method.

In one embodiment of the present invention, the composition may be used for antibacterial and antioxidant purposes.

In one embodiment of the present invention, the cosmetics include soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, body wash, shower gel, skin lotion, skin softener, skin toner, lotion, milk lotion, It may be selected from the group consisting of moisture lotion, nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, essence, nutritional essence, pack, emulsion, lipstick, makeup base, foundation, pressed powder, loose powder, and eye shadow.

The cosmetic composition of the present invention is made by bioconverting natural material extracts, and is not only harmless to the human body, but also has excellent antibacterial and antioxidant effects, so it can be usefully used in various cosmetic fields including shampoo, shower gel, and hair essence.

Figure 1 confirms the antibacterial activity of the composition of the present invention.
Figure 2 confirms the antioxidant effect of the composition of the present invention.
Figure 3 shows the results of microbial testing of shampoos and shower gels containing the composition of the present invention.
Figure 4 confirms whether heavy metals are detected in shampoo containing the composition of the present invention.
Figure 5 confirms whether heavy metals are detected in the shower gel containing the composition of the present invention.
Figure 6 shows the results of an eye mucosa test of a shampoo containing the composition of the present invention.
Figure 7 shows the results of an eye mucosa test of a shower gel containing the composition of the present invention.

Hereinafter, the present invention will be described in detail.

The present invention relates to a) a mixture containing moringa leaves, iris, goji berry, pomegranate, centella asiatica, rosemary and lemon, mixed with a first solvent containing citric acid, ascorbic acid and tyrosine and ethanol. Extracting with a second solvent each containing (Ethanol) and hydrochloric acid (HCl); and

It provides a method for producing a cosmetic composition, including the step of b) mixing and fermenting the extract extracted with the first solvent and the extract extracted with the second solvent in step a).

Moringa, irises, goji berries, pomegranates, centella asiatica, rosemary, and lemons used in the present invention can be used as whole plants or parts of plants, such as roots, stems, leaves, fruits, branches, and peels, unless there are restrictions requiring the use of specific parts of the plants. It is possible to use, and preferably, the leaves of moringa, the fruits of goji berry, pomegranate and lemon, the whole plants of irises and centella asiatica, and the stems and leaves of rosemary.

In the present invention, the mixture may include moringa leaves, iris, goji berry, pomegranate, centella asiatica, rosemary, and lemon, and the plant material may be pulverized before or after mixing.

In the present invention, dried plant material can be used.

The first solvent may include citric acid, ascorbic acid, and tyrosine, preferably 0.01 to 0.1mM citric acid, 1 to 3mM ascorbic acid, and 10 to 30uM tyrosine. It may contain, and more preferably, 0.05mM citric acid, 2mM ascorbic acid, and 20uM tyrosine. The citric acid, ascorbic acid, and tyrosine can be used by dissolving them in one or more substances selected from the group consisting of water, C ₁ to C ₄ alcohols, hexane, and mixtures thereof.

The second solvent may include ethanol and hydrochloric acid (HCl), preferably 60 to 80% ethanol and 0.005 to 0.015N hydrochloric acid, and more preferably 70% ethanol and 0.01N hydrochloric acid. May contain hydrochloric acid.

In the present invention, extract may refer to a result such as a liquid component obtained by immersing the extraction raw material in a solvent and then extracting it at low temperature, room temperature, or warm temperature for a certain period of time, or a solid component obtained by removing the solvent from the liquid component. It is not limited thereto, and may include all extracts, diluted or concentrated extracts, dried products obtained by drying the extracts, or crude or purified products thereof.

In the present invention, the extract extracted with the first solvent and the extract extracted with the second solvent can be mixed in a ratio of 1:0.5 to 2, preferably in a 1:1 ratio.

In the present invention, fermentation may be performed at 35 to 45°C for 2 to 5 days, and more preferably, fermentation may be performed for 2 days.

In the present invention, the mixture in step a) includes 25 to 35 parts by weight of moringa leaves, 15 to 25 parts by weight of iris, 5 to 15 parts by weight of goji berries, 5 to 15 parts by weight of pomegranate, and 5 to 15 parts by weight of centella asiatica, based on 100 parts by weight of the mixture. It may include 15 parts by weight, 5 to 15 parts by weight of rosemary, and 5 to 15 parts by weight of lemon, and preferably the mixture includes 30 parts by weight of moringa leaves, 20 parts by weight of calamus, and 10 parts by weight of goji berries based on 100 parts by weight of the mixture. , 10 parts by weight of pomegranate, 10 parts by weight of centella asiatica, 10 parts by weight of rosemary, and 10 parts by weight of lemon.

In the present invention, yeast (Saccharomyces cerevisiae sp.) can be used for the fermentation in step b).

In another aspect, the present invention provides a cosmetic composition prepared by a method.

The cosmetic composition of the present invention may contain not only the fermented extract of the present invention, but also ingredients commonly used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances. May include adjuvants and carriers.

In addition, the composition of the present invention can also be used by mixing conventionally used organic sunscreens as long as they do not damage the skin protection effect by reacting with the fermented extract of the present invention.

Products to which the cosmetic composition of the present invention can be added include, for example, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, body wash, shower gel, skin lotion, skin softener, and skin. Toner, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, essence, nutritional essence, pack, emulsion, lipstick, makeup base, foundation, press powder, loose powder, eye shadow, etc. It may include a formulation, and a person skilled in the art can prepare it by containing the components of the extraction mixture of the present invention based on common knowledge in the field.

The composition of the present invention may have antibacterial and antioxidant uses. In Example 3 of the present invention, it was confirmed that the composition of the present invention had excellent antibacterial activity, and in Example 4, it was confirmed that the composition of the present invention had excellent antioxidant activity.

Below, preferred embodiments are presented to aid understanding of the present invention. However, the following examples are provided only to make the present invention easier to understand, and the content of the present invention is not limited by the following examples.

Example

<Example 1: Material preparation>

Moringa was purchased from a domestic agricultural company, and goji berry, irises, pomegranate, centella asiatica, rosemary, and lemon were purchased from an online mall selling oriental medicine materials. Moringa used leaves, goji berry, pomegranate and lemon used fruit, irises and centella asiatica used whole plants, and rosemary used stems and leaves. All of the above plant materials were dried. In addition, all reagents and materials used in the present invention were commercially available.

<Example 2: Preparation of natural extract fermentation product>

300g of moringa leaves, 200g of iris, 100g of goji berry, 100g of pomegranate, 100g of centella asiatica, 100g of rosemary stems and leaves, and 100g of lemon were crushed, mixed, and then quantified (30g/dry wt./L).

Prepare a first solvent (EB01, distilled water containing 0.05mM citric acid, 2mM ascorbic acid, and 20μM tyrosine) and a second solvent (EB02, 70% acidic Ethanol, 0.01N HCL), and then grind the pulverized material with each solvent. Extraction was performed (the first and second solvents were extracted using half of the pulverized material).

The first solvent was adjusted to be 1.5 times the weight of the pulverized material, then extracted at high pressure and low temperature (1.2 Psi, 50°C) for 3 hours, and then filtered through 4 layers of gauze to prepare the extract. The filtrate was freeze-dried and used as a material. It was utilized.

The second solvent was adjusted to be 1.5 times the weight of the pulverized material, extracted by shaking (50°C, 130 rpm) for 12 hours, and then filtered through 4 layers of gauze. Next, the ethanol was evaporated by concentration under conditions of low temperature and high pressure (1.2 Psi, 50°C). The residue was removed by centrifugation (8,000 rpm/15 min, 4 h) and the supernatant was taken. Next, it was fractionated (using Filter paper #3) and the fraction was freeze-dried and used as a material.

The extract using the first solvent and the extract using the second solvent were mixed in equal amounts and incubated at about 35°C for 1 day, 2 days, 3 days, 4 days, and 5 days, respectively, using yeast strain (Saccharomyces cerevisiae sp.). By conversion, the fermented natural extract of the present invention was prepared.

<Example 3: Antibacterial activity assay>

The antibacterial activity of the fermented product prepared in Example 2 was confirmed using P. acne.

To measure antibacterial activity, the fermented and dried composition of the present invention was suspended in physiological saline solution, mixed with a test bacterial solution, left at room temperature for 5 minutes, and the antibacterial activity was measured over time in an incubator. The test solution was diluted step by step, and 1 mL of each concentration was dispensed into two Petri dishes. The prepared (45-50) ℃ agar was dispensed (15-25) mL into the Petri dishes, and allowed to solidify at room temperature. The coagulated Petri dish was inverted and cultured at (35 ± 1) °C for (24 to 48) hours to observe the number of viable cells. The number of viable bacteria was calculated according to (Equation 1), and the sterilization reduction rate was determined according to (Equation 2).

[Equation 1] Calculation of viable cell count

N = C × D

N: Number of viable bacteria, C: Number of colonies (average number of colonies on 2 sheets), D: Dilution factor

[Equation 2] Calculation of antibacterial effect (%)

R(%) = [(A - B) / A] × 100

R: Antibacterial effect, A: Initial viable cell count (average) in control group,

B: Number of viable bacteria in the test group after a certain period of time (average)

As a result, as shown in Figure 1, the antibacterial activity showed significant growth inhibition after 5 days at a concentration of 2%, and the effect depending on the incubation time was somewhat higher at 48 hours.

<Example 4: Antioxidant activity assay>

The antioxidant effect was measured by reducing power using free radical free radicals using DPPH (2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl).

The DPPH test method is a representative experiment to check the scavenging ability of free radicals. 1 mL of ethanol is added to 1 mL of 0.2mM DPPH solution dissolved in methanol, 1 mL of fermented product (extract = sample) is added, mixed, and then incubated at room temperature for 10 minutes. After standing for a minute, the absorbance was measured at 517 nm using a spectrophotometer. Antioxidant activity was measured by using the case where no sample was added as the control group and the case where the sample was added as the experiment group, using the following equation to measure the inhibition rate of the scavenging activity of DPPH.

[Equation 3]

Inhibition (%) = {1 - [(AExperiment - ABlank)/ AControl]} × 100

As a result, as shown in Figure 2, the free radical scavenging ability increased as the concentration of the moringa extract yeast fermentation product increased, and it was confirmed that at a concentration of 4%, it was about 75% of the 25 μg/ml vitamin C used as a standard substance.

<Example 5: Manufacturing a product using fermented natural extract>

In order to confirm the suitable formulation for the three body types including shampoo/shower gel/hair essence using natural materials, we slightly modified our basic formulation and prepared the formulation by mixing each composition as follows.

Ingredients (shampoo composition) Weight ratio (%) Cosmetic composition of the present invention 8.0 Hydrolyzed Keratin 1.5 Hydrolyzed Collagen 1.5 Dexpanthenol 1.2 L-Menthol 0.3 Disodium Laureth Sulfosuccinate 0.8 Decyl Glucoside 0.1 Cocamidopropyl Betaine 0.2 Disodium EDTA 0.15 PEG-120 Methyl Glucose Dioleate 0.25 Polyquaternium-10 0.1 1,2-hexanediol 1.0 Citric Acid 0.8 Glyceryl Caprylate 3.0 Salicylic Acid 1.0 Spices 0.1 Purified water balance Sum 100

Ingredients (Shower Gel) Weight ratio (%) Cosmetic composition of the present invention 9.0 Lauryldiethanolamide 6.0 Polyoxyethylene lauryl ether sulfate sodium solution 30 Sodium lauryl sulfate solution 15 propylene glycol 1.0 Methylparahydroxybenzoate 0.2 Spices 0.5 citric acid 1.3 Purified water balance Sum 100

Ingredients (Hair Essence) Weight ratio (%) Cosmetic composition of the present invention 10 Polysorbate 60 0.5 Trehalose 0.8 Dexpanthenol 0.1 carbomer 0.15 xanthan gum 1.5 Hydrolyzed Keratin 2.0 Hydrolyzed Elastane 1.5 Hydrolyzed Collagen 1.5 Disodium EDTA 0.15 Glyceryl Caprylate 0.1 Salicylic Acid 0.5 arginine 0.8 L-Menthol 0.3 Sodium Hyraluronate 1.5 Spices 0.1 Purified water balance Sum 100

In order to confirm the safety of the completed formulation and composition, a specialized clinical laboratory was commissioned to conduct tests for microorganisms, heavy metals, and ocular mucosa on two types of shampoo and shower gel. Microbial and heavy metal test results through clinical trials were all judged “non-detectable” (Figures 3 to 5).

In the ocular mucosa stability test, no bleeding reaction, melting, or coagulation reaction was observed. Therefore, the irritation index (S score) for 6 fertilized eggs for both shampoo and shower gel was calculated as '16' for the undiluted solution, corresponding to 'Severely irritating', and for the 10% concentration, it was calculated as '15', indicating 'Irritating'. ', in the case of 1% concentration, it was calculated as '3', confirming that it corresponds to 'Nonirritant or Slightly irritating'.

In the case of the negative control group, the irritation index (S score) for 6 fertilized eggs was '0', confirming that it corresponds to 'Nonirritant or Slightly irritating', and in the case of the positive control group, the irritation index (S score) for 6 fertilized eggs was confirmed to be '0'. was calculated as '15', confirming that it corresponds to 'Irritating'. From the above results, when evaluating eye mucosa irritation for shampoos and shower gels using the HET-CAM test method, the undiluted solution is classified as 'Severely irritating', 10% concentration is 'Irritating', and 1% concentration is 'Nonirritant or Slightly irritating'. It was confirmed that it was considered a substance (Figures 6 and 7).

Claims (6)

a) Moringa leaves;
Goji berries, pomegranate fruits and lemon fruits;
Iris outpost and Centella asiatica outpost; and
The mixture containing rosemary stems and leaves is mixed with a first solvent containing citric acid, ascorbic acid, and tyrosine and a second solvent containing ethanol and hydrochloric acid (HCl), respectively. Extracting step; and
b) A method of producing a cosmetic composition comprising the step of mixing and fermenting the extract extracted with the first solvent and the extract extracted with the second solvent in step a).
The method of claim 1, wherein the mixture in step a) contains 25 to 35 parts by weight of moringa leaves, 15 to 25 parts by weight of calamus whole plant, 5 to 15 parts by weight of goji berry fruit, and 5 to 15 parts by weight of pomegranate fruit, based on 100 parts by weight of the mixture. Part, 5 to 15 parts by weight of centella asiatica whole plant, 5 to 15 parts by weight of rosemary stems and leaves, and 5 to 15 parts by weight of lemon fruit.
The method of claim 1, wherein the fermentation in step b) uses yeast (Saccharomyces cerevisiae sp.).
A cosmetic composition prepared by the method of any one of claims 1 to 3.
The cosmetic composition of claim 4, wherein the composition is for antibacterial and antioxidant purposes.
The method of claim 4, wherein the cosmetics include soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, body wash, shower gel, skin lotion, skin softener, skin toner, lotion, milk lotion, and moisture lotion. A cosmetic composition selected from the group consisting of nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, essence, nutritional essence, pack, emulsion, lipstick, makeup base, foundation, pressed powder, loose powder, and eye shadow.