KR20220108828A - 저하된 상위 및 하위 운동 뉴런 기능 및 감각 지각을 나타내는 비-인간 동물 - Google Patents

저하된 상위 및 하위 운동 뉴런 기능 및 감각 지각을 나타내는 비-인간 동물 Download PDF

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KR20220108828A
KR20220108828A KR1020227024994A KR20227024994A KR20220108828A KR 20220108828 A KR20220108828 A KR 20220108828A KR 1020227024994 A KR1020227024994 A KR 1020227024994A KR 20227024994 A KR20227024994 A KR 20227024994A KR 20220108828 A KR20220108828 A KR 20220108828A
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부르신 이키즈
마이클 라크루아-프랄리시
수잔 디. 크롤
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리제너론 파마슈티칼스 인코포레이티드
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Abstract

유전자 변형 DR6 대립유전자를 포함하고 출생시 및 출생 후 몇 주 또는 몇 개월 동안 정상적인 표현형을 나타내는 유전자 변형 비-인간 동물을 포함하는 것으로서, 근 위축성 측삭 경화증 (ALS)과 같은 질환의 운동 뉴런 기능 장애를 위한 동물 모델. 그러나 비-인간 동물은 나이가 듦에 따라, 하나 이상의 ALS 유사 증상으로 나타나는 운동 뉴런 기능 장애가 발생하며, 이는 발병 후 빠르게 진행될 수 있다. ALS를 억제, 지연 또는 치료하는데 사용될 수 있는 후보 제제를 확인하는 방법이 또한 제공된다.

Description

저하된 상위 및 하위 운동 뉴런 기능 및 감각 지각을 나타내는 비-인간 동물{NON-HUMAN ANIMAL EXHIBITING DIMINISHED UPPER AND LOWER MOTOR NEURON FUNCTION AND SENSORY PERCEPTION}
관련 출원의 상호 참조
본 출원은 (2015년 3월 16일자로 출원된) 미국 가출원 제62/133,909호 및 (2015년 11월 3일자로 출원된) 미국 가출원 제62/250,229호에 대한 이익을 주장하며, 이들 각각의 출원은 본 명세서에 참고로 포함된다.
서열 목록
서열 목록의 공식 사본은 2016년 3월 16일자로 작성되고 크기가 약 685 킬로바이트인 2016-03-16-T0041WO01-SEQ-LIST_ST25.txt의 파일명의 ASCII 포맷 서열 목록으로서 EFS-웹을 통해 전자적으로 제출되며, 본 명세서와 동시에 제출된다. 이러한 ASCII 포맷 문서에 포함된 서열 목록은 본 명세서의 일부이며, 전체적으로 본 명세서에 참고로 포함된다.
기술분야
본 출원은 일반적으로 시간 경과에 따라 상위 운동 뉴런, 하위 운동 뉴런 및/또는 감각 지각의 저하가 발생하는 비-인간 동물로서, 신경 퇴행성 장애, 예를 들어 근 위축성 측삭 경화증 (ALS)과 같은 운동 뉴런 질환에 유용한 모델을 제공할 수 있는 비-인간 동물에 관한 것이다.
루게릭병 (Lou Gehrig's Disease)으로 알려진 근 위축성 측삭 경화증 (ALS)은 수의 운동을 담당하는 척수, 뇌간 및 피질의 운동 뉴런의 파괴로 인한 치명적 신경 퇴행성 질환이다. 이 질환은 임상적으로 진행성 근육 약화와 위축으로 나타나, 발병 후 3 내지 5년 이내에 마비와 사망을 초래한다.
미국에서는 대략 2만 명이 ALS를 앓고 있으며, 매년 5,000 명이 ALS로 진단된다. ALS는 전 세계의 모든 인종 및 민족 배경의 사람들에서 공통적으로 발병된다. ALS가 발병하는 평균 연령은 40세에서 60세 사이이나, ALS는 젊은 연령층과 노년층의 남성과 여성 모두에서 발생할 수 있다. ALS 사례의 90 내지 95%에서, 상기 질환은 명백히 무작위적이다 (산발성 ALS (sALS)로 알려짐). 이러한 sALS 사례에서, 상기 질환은 가족력이 없으며, 명확하게 관련된 위험 인자가 없다. ALS 사례의 5 내지 10%에서는 선천성 유전적 연결이 존재한다 (가족성 ALS (fALS)로 알려져 있음).
ALS와 관련된 돌연변이 중 구리 아연 초과산화물 불균등화 효소 (SOD1) 유전자의 돌연변이는 오랫동안 SOD1 효소의 항산화 기능의 손상보다는 기능의 독성 증가를 통해 ALS 질환을 일으키는 것으로 생각되어 왔다. fALS와 관련된 돌연변이를 가진 다른 유전자로는 알신 (alsin) (ALS2), 세나탁신 (senataxin) (ALS4), 소포 관련 막 단백질 (VAPB, ALS8), 안지오제닌 (Angiogenin), 및 다이낙틴 (dynactin)의 p150 하위 단위 (DCTN1)가 포함된다. 최근, 분명한 가족력이 있거나 그렇지 않은 ALS 환자에서 Tardbp의 TDP-43-코딩 영역에 30개 초과의 돌연변이가 확인되었으며, 이는 대략 4%의 fALS 및 1% 미만의 sALS에 상응하였다. TDP-43 돌연변이(들)를 가진 대부분의 환자는 인지 결손이 없는 고전적인 ALS 표현형을 나타내며, 이는 ALS 발생에서의 TDP-43의 중요한 역할을 시사한다. 또한, 연장된 GGGGCC 헥사뉴클레오티드는 C9ORF72 유전자의 프로모터에서 반복되고, fALS 및 sALS뿐만 아니라 ALS 관련 전두측두성 치매 (ALS-FTD)의 매우 공통적인 원인으로 나타난다.
SOD1, TDP43 또는 FUS, ALS2-녹아웃 마우스 및 뉴로필라멘트 하위 단위를 코딩하는 유전자 조작된 유전자를 갖는 마우스에서 돌연변이를 갖는 설치류 계통을 포함하는 다수의 마우스 모델이 ALS 질환에 대해 수립되었다. 이들 중, 인간 돌연변이형 SOD1 (mSOD1) 트랜스제닉 마우스 모델은, ALS 환자와 몇몇 임상적 표현형을 공유하기 때문에, 현재 가장 널리 사용되는 모델이다. mSOD1 마우스의 첫 번째 증상은 하나 이상의 팔다리의 미세한 "떨림 (jittering)/진전 (tremor)"으로, 대략 90일 내지 100일령에 나타난다. 후기 단계에서, 마우스는 근육 약화 및/또는 뒷다리의 불완전 마비로 임상 경과가 개시된 후, 그 다음에 앞다리가 불완전 마비되고, 마지막으로 중증 사지 마비가 유발된다. 그러나, 현재의 동물 모델은, 동물이 상위 운동 뉴런 증상, TDP43 및/또는 SOD1 응집체 및/또는 비-운동 뉴런 상실을 나타내지 않는다는 점에서 인간의 질환으로 해석되지 못한다. 따라서, 인간의 ALS를 더욱 근접하게 반영하는 동물 모델이 요구된다.
상위 운동 뉴런, 하위 운동 뉴런 및/또는 감각 지각의 기능 장애 증상의 병증이 발생하고, 이와 같이, 근 위축성 측삭 경화증 (ALS), 원발성 측삭 경화증, 원발성 측삭 경화증, 진행성 근이영양증 등과 같은 운동 뉴런에 유발된 하나 이상의 신경 퇴행성 질환의 모델로서 유용할 수 있는 유전자 변형 비-인간 동물이 본 명세서에 제공된다. 구체적으로, 변형 내인성 DR6 유전자좌를 포함하는 래트 또는 마우스와 같은 설치류로서, 상기 변형 DR6 유전자좌는, DR6 세포질 사멸 도메인 또는 DR6 세포질 도메인 전체를 인코딩하는 제1 뉴클레오티드 (예를 들어, 제1 DNA, 제1 cDNA, 제1 게놈 DNA 등) 서열이 결여된 (예를 들어, 포함하지 않는, 부재하는 등) 설치류, 예를 들어, 상기 변형 DR6 유전자좌는, DR6 세포질 사멸 도메인 또는 DR6 세포질 도메인의 임의의 일부분을 인코딩하는 뉴클레오티드 서열이 결여된 설치류가 제공된다. 상기 변형 DR6 유전자좌는 (i) DR6 막 관통 도메인 전체를 인코딩하는 제2 뉴클레오티드 (예를 들어, 제2 cDNA, 제2 DNA, 제2 게놈 DNA) 서열, 예를 들어 DR6 막 관통 도메인의 임의의 일부분을 인코딩하는 제2 뉴클레오티드 서열; (ii) DR6 세포 외 도메인 또는 그의 임의의 일부분을 인코딩하는 제3 뉴클레오티드 (예를 들어, 제3 cDNA, 제3 DNA, 제3 게놈 DNA) 서열, 예를 들어, DR6 세포 외 도메인의 임의의 일부분을 인코딩하는 제3 뉴클레오티드 서열; (iii) 제4 뉴클레오티드 서열, 예를 들어, 내인성 설치류 DR6 유전자의 개재 인트론을 포함하여, 3번 엑손 전체에서 6번 엑손 전체에 걸친 제4 게놈 서열; 및/또는 (iv) 제5 뉴클레오티드 서열, 예를 들어, 내인성 설치류 DR6 유전자의 개재 인트론을 포함하여, 2번 엑손의 일부, 예를 들어 4103번 염기에서 6번 엑손의 전체, 예를 들어 종결 코돈에 걸친 제4 게놈 서열이 추가로 결여될 수 있으며, 제5 뉴클레오티드 서열은 전장의 성숙 내인성 설치류 DR6 단백질을 인코딩한다. 일부 실시 형태에서, 상기 설치류는 마우스이고, 상기 변형 DR6 대립유전자는 DR6 세포질 사멸 도메인을 인코딩하는 제1 뉴클레오티드 서열이 결여되어 있으며, 예를 들어, 상기 변형 DR6 대립유전자는 서열 번호 15의 아미노산 388 내지 454 또는 그의 임의의 일부분을 인코딩하는 제1 뉴클레오티드 서열이 결여되어 있다. 일부 실시 형태에서, 세포질 도메인, 예를 들어 서열 번호 15의 아미노산 330 내지 614로 제시된 아미노산 서열을 가지는 세포질 도메인을 인코딩하는 뉴클레오티드 서열이 결여된 변형 DR6 대립유전자를 포함하는 마우스가 제공된다. 일부 실시 형태에서, 본 명세서에 제공된 마우스는, 예를 들어 서열 번호 15의 아미노산 1 내지 614로 제시된 바와 같은 성숙 DR6 단백질을 인코딩하는 뉴클레오티드 서열이 결여된 변형 DR6 대립유전자를 포함한다. 일부 실시 형태에서, 상기 변형 DR6 대립유전자는 5 kb 초과의 내인성 게놈 서열이 결여되어 있다. 일부 실시 형태에서, 상기 변형 DR6 대립유전자는 10 kb 초과의 내인성 게놈 서열이 결여되어 있다. 일부 실시 형태에서, 상기 변형 DR6 대립유전자는 20 kb 초과의 내인성 게놈 서열이 결여되어 있다. 일부 실시 형태에서, 상기 변형 DR6 대립유전자는 30 kb 초과의 내인성 게놈 서열이 결여되어 있다. 일부 실시 형태에서, 상기 변형 DR6 대립유전자는 40 kb 초과의 내인성 게놈 서열이 결여되어 있다. 일부 실시 형태에서, 상기 변형 DR6 대립유전자는 50 kb 초과의 내인성 게놈 서열이 결여되어 있다. 일부 실시 형태에서, 상기 설치류는 내인성 DR6 세포 외 도메인, 내인성 막 관통 도메인 및/또는 내인성 DR6 세포질 도메인의 임의의 부분을 인코딩하는 뉴클레오티드 서열이 결여된 변형 DR6 유전자좌에서 이형접합성 또는 동형접합성이다.
본 명세서에 제공된 바와 같은 유전자 변형 설치류는, 예를 들어 내인성 DR6 유전자좌 (예를 들어, 본 명세서에 기재된 바와 같은 변형 내인성 DR6 유전자좌)에서 내인성 DR6 전사 조절 서열에 작동가능하게 연결될 수 있거나 또는 설치류 게놈에 무작위로 삽입될 수 있는 핵산을 발현할 수 있으며, 상기 핵산은 기능성 DR6 신호 펩티드 (예를 들어, 기능성 이종성 DR6 신호 펩티드, 기능성 내인성 DR6 신호 펩티드, 기능성 래트 DR6 신호 펩티드, 기능성 마우스 DR6 신호 펩티드 등), 막 관통 도메인 (예를 들어, DR6 막 관통 도메인 또는 이종성 막 관통 도메인 (예를 들어, ROR1 막 관통 도메인)), 리포터 단백질 (예를 들어, β-갈락토시다제 단백질) 또는 이들의 조합을 포함한 폴리펩티드를 인코딩하고, 상기 폴리펩티드는 기능성 DR6 세포질 도메인 또는 그의 임의의 일부분이 결여되어 있다 (예를 들어, 작동가능하게 융합되어 있지 않거나, 포함하지 않는 등). 일부 실시 형태에서, 상기 폴리펩티드는 기능성 DR6 세포 외 도메인 또는 그의 임의의 일부분이 또한 결여되어 있다. 일부 실시 형태에서, 상기 폴리펩티드는 기능성 DR6 신호 펩티드로 본질적으로 이루어지거나 또는 그로 이루어진다. 일부 실시 형태에서, 상기 폴리펩티드는 막 관통 도메인에 작동가능하게 융합된 기능성 DR6 신호 펩티드로 본질적으로 이루어지거나 또는 그로 이루어진다. 일부 실시 형태에서, 상기 폴리펩티드는, 리포터 단백질에 작동가능하게 융합된 막 관통 도메인에 작동가능하게 융합된 기능성 DR6 신호 펩티드로 본질적으로 이루어지거나 또는 그로 이루어진다. 일부 실시 형태에서, 상기 폴리펩티드는 막 관통 도메인에 작동가능하게 융합된 기능성 DR6 신호 펩티드로 본질적으로 이루어지거나 또는 그로 이루어진다. 일부 실시 형태에서, 상기 폴리펩티드는 막 관통 도메인으로 본질적으로 이루어지거나 또는 그로 이루어진다. 일부 실시 형태에서, 상기 폴리펩티드는 리포터 단백질에 작동가능하게 융합된 막 관통 도메인으로 본질적으로 이루어지거나 또는 그로 이루어진다. 일부 실시 형태에서, 상기 폴리펩티드는 리포터 단백질로 본질적으로 이루어지거나 또는 그로 이루어진다. 일부 실시 형태에서, 상기 DR6 신호 펩티드는, 예를 들어 서열 번호 15의 아미노산 -1 내지 -41 (또는, 그의 임의의 일부분)로 제시되는 바와 같은 설치류 DR6 신호 펩티드, 예를 들어, 마우스 DR6 신호 펩티드이며, 예를 들어 서열 번호 6에 제시된 서열 또는, 동일한 아미노산 서열을 인코딩하나 단지 상기 유전자 코드의 축퇴 (degeneracy)로 인해 서열 번호 6과 상이한 그의 축퇴 변이체에 의해 인코딩된다. 일부 실시 형태에서, 본 명세서에 제공된 바와 같은 설치류는, 서열 번호 6으로 제시된 서열 또는, 동일한 아미노산 서열을 인코딩하나 단지 상기 유전자 코드의 축퇴로 인해 서열 번호 6과 상이한 그의 축퇴 변이체를 포함하거나, 이들로 본질적으로 이루어지거나 또는 이들로 이루어진 핵산을 포함하며 이를 발현시킨다. 일부 실시 형태에서, 상기 막 관통 도메인은 ROR1 막 관통 도메인이며, 예를 들어, 서열 번호 7로 제시된 서열 또는, 동일한 아미노산 서열을 인코딩하나 단지 상기 유전자 코드의 축퇴로 인해 서열 번호 7과 상이한 그의 축퇴 변이체에 의해 인코딩된다. 일부 실시 형태에서, 상기 핵산 서열은, 서열 번호 7로 제시된 서열 또는, 동일한 아미노산 서열을 인코딩하나 단지 상기 유전자 코드의 축퇴로 인해 서열 번호 7과 상이한 그의 축퇴 변이체를 포함하거나, 이들로 본질적으로 이루어지거나 또는 이들로 이루어진다. 일부 실시 형태에서, 리포터 단백질은 β-갈락토시다제이며, 예를 들어 서열 번호 8로 제시된 서열 또는, 동일한 아미노산 서열을 인코딩하나 단지 상기 유전자 코드의 축퇴로 인해 서열 번호 8과 상이한 그의 축퇴 변이체에 의해 인코딩된다. 일부 실시 형태에서, 상기 핵산 서열은, 서열 번호 8 또는, 동일한 아미노산 서열을 인코딩하나 단지 상기 유전자 코드의 축퇴로 인해 서열 번호 8과 상이한 그의 축퇴 변이체를 포함하거나, 이들로 본질적으로 이루어지거나 또는 이들로 이루어진다. 일부 실시 형태에서, 본 명세서에 제공된 바와 같은 설치류는, β-갈락토시다제에 작동가능하게 융합된 ROR1 막 관통 도메인에 작동가능하게 융합된 DR6 신호 펩티드를 인코딩하는 핵산을 포함하며 이를 발현시키고, 예를 들어, 본 명세서에 제공된 바와 같은 설치류는, 서열 번호 17로 제시된 핵산 서열 또는, 동일한 아미노산 서열을 인코딩하나 단지 상기 유전자 코드의 축퇴로 인해 서열 번호 17과 상이한 그의 축퇴 변이체를 포함하며 이를 발현시킨다.
일부 실시 형태에서, 상기 핵산은 내인성 DR6 유전자좌에서, 하나 이상의 내인성 DR6 조절 요소, 예를 들어 내인성 전사 조절 요소에 선택적으로 작동가능하게 연결되어, 상기 설치류가, 상기 핵산을 포함하나, 적어도, 내인성 DR6 세포질 도메인 전체 또는 그의 임의의 일부분 전체 (예를 들어, DR6 세포질 사멸 도메인 전체)를 인코딩하는 뉴클레오티드 서열이 결여되고, 선택적으로, 내인성 DR6 막 관통 도메인 또는 그의 임의의 일부분 전체 및/또는 내인성 DR6 세포 외 도메인 전체를 인코딩하는 추가의 뉴클레오티드 서열이 추가로 결여된 변형 DR6 유전자좌에서 이형접합성 또는 동형접합성이 된다.
일부 실시 형태에서, 본 명세서에 기재된 바와 같은 내인성 DR6 유전자좌의 변형 및/또는 상기 핵산의 발현은 뉴런, 신경교세포 또는 이들의 조합의 기능에 영향을 미친다. 일부 실시 형태에서, 상기 핵산의 발현은 동일한 유전적 배경, 예를 들어 상기 유전자 변형 설치류와 동일한 계통인 대조군 설치류와 비교하여, 예를 들어 (a) 척추후만증 (kyphosis); (b) 비정상적인 뒷다리 움켜쥐기; (c) 운동 협응과 운동 학습 능력의 결손, (d) 중량 감소 및 (e) 감각 지각의 저하로 나타날 수 있는 상위 및/또는 하위 운동 뉴런의 기능의 저하, 예를 들어 기능 장애를 초래한다. 일부 실시 형태에서, 본 명세서에 기재된 바와 같은 변형 DR6 유전자좌를 포함하고/하거나 상기 핵산을 발현하는 설치류는 운동 뉴런 기능 장애, 예를 들어 상위 운동 뉴런 및/또는 하위 운동 뉴런 기능 장애의 하나 이상의 증상을 나타낼 수 있다. 일부 실시 형태에서, 상기 증상은, 동일한 유전적 배경, 예를 들어 상기 유전자 변형 설치류와 동일한 계통인 대조군 설치류와 비교하여, (a) 척추후만증; (b) 비정상적인 뒷다리 움켜쥐기; (c) 운동 협응과 운동 학습 능력의 결손, (d) 중량 감소 및/또는 (e) 감각 지각의 저하이다. 일부 실시 형태에서, 상기 하나 이상의 증상은 진전, 경직성 마비 (강직), 비정상적인 반사 및 이들의 조합으로 이루어진 군으로부터 선택되는 상위 운동 뉴런 기능 장애를 포함한 ALS 유사 증상 및/또는 근력 약화와 소모, 근섬유다발수축, 및 이들의 조합으로 이루어진 군으로부터 선택되는 하위 운동 뉴런 기능 장애를 포함한 ALS 유사 증상이다. 이러한 ALS 유사 증상은 블라인드 주관적 ALS-TDI 신경학적 점수화(blinded subjective ALS-TDI neurological scoring), 로타로드 테스트(rotarod testing), 캣워크 테스트(catwalk testing), 오픈 필드 테스트(open field testing) 중 하나 이상을 사용하여, 확인될 수 있다. 또한, 본 명세서에 개시된 바와 같은 설치류는 8주령 내지 20주령의 대조군 야생형 동물과 비교하여 중량 증가의 감소를 나타낼 수 있다. 본 명세서에 개시된 바와 같은 설치류는, 상기 설치류가 로타로드 위에서의 시간의 결손, 보행성 운동 기능의 손상, 캣워크 능력의 저하 및 이들의 조합으로 나타나는 운동 뉴런 기능의 저하를 나타내는지 또는 그러하지 않은지에 관계없이, 통증 자극에 대한 반응 지체 시간의 결손을 또한 나타낼 수 있다.
일 태양에서, 상기 설치류는 출생 시 지극히 정상적일 수 있으며 (예를 들어, 육안으로 볼 때, 정상적으로 보이고, 예를 들어, 운동 뉴런 기능 장애 및/또는 감각 지각의 저하가 블라인드 주관적 신경학적 점수화 검정, 로타로드 테스트, 캣워크 테스트, 오픈 필드 테스트, 중량 측정 및/또는 통증 종속 시험 (pain subjection test)에서 분명하지 않음), 나이가 듦에 따라, 하나 이상의 가시적인 ALS 유사 증상이 발생하고, 예를 들어, 상기 설치류는 2주령, 3주령, 4주령, 5주령, 6주령 등 이후, 하나 이상의 ALS 유사 증상이 발생할 수 있다. 일 태양에서, 본 명세서에 기재된 바와 같은 설치류는 4주령 미만이며, 어떠한 ALS 유사 증상도 나타내지 않는다. 일 태양에서, 본 명세서에 기재된 바와 같은 설치류는 8주령 미만이며, 어떠한 ALS 유사 증상도 나타내지 않는다. 다른 태양에서, 상기 설치류는 22주령 이전에 ALS 유사 증상이 발생한다. 일 실시 형태에서, 상기 설치류는 적어도 16주령 이상이며, 대조군 설치류와 비교하여 하기 표현형 중 하나 이상을 나타낸다: (a) 척추후만증; (b) 비정상적인 뒷다리 움켜쥐기; (c) 운동 협응과 운동 학습 능력의 결손, (d) 중량 감소, 및 (e) 통증 자극에 대한 반응 지체 시간의 지연.
본 명세서에 개시된 설치류의 유전자 발현 패턴, 예를 들어, 신경계 기관 (뇌, 척수 등)의 유전자 특징은 (1) 각각, ALS를 앓는 환자의 유전자 발현 패턴, 또는 상기 환자의 기관 (뇌, 척수 등)의 유전자 특징, 및/또는 (2) 각각, ALS의 다른 동물 모델, 예를 들어, SOD1 비-인간 동물의 유전자 발현 패턴, 또는 상기 비-인간 동물의 기관 (뇌, 척수 등)의 유전자 특징과 유사할 수 있다. 예를 들어, 본 명세서에 개시된 설치류의 뇌 및/또는 척수의 유전자 발현 패턴(들)은 인간 ALS 바이오세트 및/또는 쥣과 동물 SOD1 바이오세트와 상관관계가 존재할 수 있으며, 이는 면역 반응과 연결될 수 있다. 인간 ALS 및/또는 쥣과 동물 SOD1 바이오세트와의 상관관계는, ALS 또는 SOD1 동물 모델을 사용하여, 본 명세서에 개시된 설치류에서 관찰된 병리학이 인간에서 볼 수 있는 것과 매우 유사함을 시사한다. 일부 실시 형태에서, 본 명세서에 개시된 바와 같은 설치류는 면역 반응에 연결된 유전자 특징을 가질 수 있으나, 말초의 경우, 야생형 설치류와 비교하여 비정상적인 면역 반응을 나타내지 않을 수 있다.
일 태양에서, 본 명세서에 개시된 바와 같은 설치류의 운동 뉴런의 수는 야생형 설치류의 운동 뉴런의 수와 유사하며, 예를 들어, 현저하게 크거나 작지 않다. 일부 실시 형태에서, 본 명세서에 개시된 바와 같은 설치류의 운동 뉴런은 야생형 설치류의 운동과 비교하여 증가된 산화 스트레스를 나타낸다.
일 태양에서, 본 명세서에 기재된 바와 같은 설치류는 래트 또는 마우스이다. 일부 실시 형태에서, 상기 설치류는 129 계통, C57BL/6 계통, 및 혼합된 C57BL/6 x 129 계통으로 이루어진 군으로부터 선택된 계통의 마우스이다.
또한, 본 명세서에 기재된 설치류로부터 단리되거나 유래된 조직 또는 세포, 예를 들어 배아 줄기 세포, 운동 뉴런 세포 등이 본 명세서에 제공되며, 이는 예를 들어, 조직학적으로 검사되고/되거나 배양될 수 있다. 또한, 본 명세서에 기재된 핵산이 제공된다.
또한, 본 명세서에 기재된 설치류로부터 단리된 세포 또는 세포 집단을 배양하는 단계를 포함하는 방법이 제공되며, 이는 예를 들어, 시험관 내(in vitro) 조작에 유용한 운동 뉴런을 생성할 수 있다. 일부 실시 형태에서, 대조군 운동 뉴런 세포와 비교하여 증가된 산화 스트레스를 나타내는 운동 뉴런 집단을 제조하는 방법이 제공되며, 상기 방법은 본 명세서에 제공된 설치류로부터 유래된 배상체를 수립하는 단계 및 상기 배상체를 운동 뉴런으로 분화시키는 단계를 포함한다. 일부 실시 형태에서, 배상체는 내세포집단 배아 줄기 세포로부터 발생된다. 일부 실시형태에서, 내세포집단 배아 줄기 세포를 단리하고, 예를 들어 배아 줄기 세포 배지 (ESM: DMEM + 15% 소 태아 혈청 + 페니실린/스트렙토마이신 + 글루타민 + 비 필수 아미노산 + 뉴클레오시드 + β-메르캅토에탄올 + 피루브산나트륨 + LIF)에서 2일 동안 배양하여, 배양체를 형성시킨다. 상기 배양체를, 예를 들어, 레티노산 및 평활화 작동제에서, 예를 들어 5일 동안 추가로 배양하기 전에 1 내지 3일, 예를 들어 2일 동안, 분화 배지 (최신 DMEM/F12 + 뉴런세포 기본 배지 + 10% 녹아웃 혈청 + 페니실린/스트렙토마이신 + 글루타민 + β-메르캅토에탄올)에서 후속하여 배양할 수 있다. 본 명세서에 제공된 설치류에서 단리된 내세포집단 배아 줄기 세포로부터 발생된 분화 운동 뉴런을, 예를 들어 배아 줄기 세포-유래 운동 뉴런 배지 (ESMN: 뉴런세포 기본 배지 + 2% 말 혈청 + B27 + 글루타민 + 페니실린/스트렙토마이신 + β-메르캅토에탄올 + 10 ng/ml GDNF, BDNF, CNTF)에서 성숙시켜, 상기 변형 DR6 대립유전자 및/또는 핵산을 포함하지 않는 설치류에서 발생된 운동 뉴런 세포와 비교하여 증가된 산화 스트레스를 나타내는 안정한 운동 뉴런 세포주를 형성시킬 수 있다.
또한, ALS를 치료, 억제 및/또는 저해하는 데 유용할 수 있는 것으로서, 운동 뉴런 기능 장애를 조절하기 위한 후보 제제를 확인하는 방법으로서, (a) 본 명세서에 개시된 설치류에 상기 제제를 투여하는 단계; 및 (b) 동일한 게놈 구조를 가지나 상기 후보 제제가 제공되지 않은 시험 대조군 설치류와 비교하여, 상기 설치류에서의 상기 후보 제제의 효과를 결정하는 단계, 예를 들어, 대조군 설치류와 비교하여, 상기 제제가 상기 설치류에서 ALS 유사 증상 중 적어도 하나를 억제, 저해, 지연 및/또는 역전시키는지의 여부를 결정하는 단계를 포함하고; 상기 설치류에서, 하나 이상의 ALS 유사 증상, 예를 들어 척추후만증, 비정상적인 뒷다리 움켜쥐기, 운동 협응 결손, 운동 학습 능력 결손, 중량 감소 및 감각 지각 결손과 같은 운동 뉴런 기능 장애의 증상의 억제, 저해, 지연 및/또는 역전은 운동 뉴런 기능 장애의 치료, 예를 들어 ALS의 억제 및/또는 저해에 유용할 수 있는 후보 제제를 나타내는 방법이 본 명세서에 제공된다. 상기 제제는 상기 설치류에서 ALS 유사 증상의 발병 전에, 그와 동시에 또는 그 후에, 예를 들어 블라인드 주관적 ALS-TDI 신경학적 점수화, 로타로드 테스트, 캣워크 테스트, 오픈 필드 테스트, 중량 측정 또는 통증 자극에 대한 반응 지체 시간의 결정에 의한 운동 뉴런 기능 장애의 하나 이상의 증상의 검출 전에, 그와 동시에 또는 그 후에, 예를 들어 둘 이상의 상이한 시점에 투여될 수 있다.
일부 실시 형태에서, 상기 후보 제제는 운동 뉴런 기능 장애의 적어도 하나의 증상을, 예를 들어, 10% 이상, 예를 들어, 15% 이상, 예를 들어, 20% 이상 조절한다. 다른 실시 형태에서, 상기 후보 제제의 존재는 운동 뉴런 기능 장애의 적어도 하나의 증상을, 예를 들어, 50% 이상, 예를 들어, 75% 이상, 예를 들어, 80% 이상, 예를 들어, 95% 이상, 예를 들어, 99% 이상 조절한다. 일부 실시 형태에서, 상기 후보 제제는 운동 뉴런 기능 장애의 적어도 하나의 증상을 억제한다. (1) 척추후만증 및 비정상적인 뒷다리 움켜쥐기 중 적어도 하나의 억제 및/또는 저해 및/또는 (2) 운동 협응 결손, 운동 학습 능력 결손, 중량 감소 및/또는 감각 지각 결손의 억제 및/또는 회복에 의해 상기 적어도 하나의 증상을 조절하는 후보 제제가 신경퇴행성 질환, 예를 들어 ALS의 치료에 유용할 수 있다.
상기 제제가 상기 설치류에서, 대조군 설치류와 비교하여, ALS 유사 증상 중 적어도 하나를 억제, 저해, 지연 및/또는 역전시키는지의 여부를 결정하는 방법은 상기 설치류에서 단리된 조직 및/또는 세포를 검사하는 단계, 예를 들어, 조직화학; 세포, 조직 및/또는 기관 배양, 현미경 기법 및/또는 단백질, 예를 들어 마이엘린 결합 단백질 (MBP), 신경생장인자수용체 (NGFR), 콜린 아세틸트랜스퍼라제 (Chat), Mnx 호메오박스, 글루타메이트 [NMDA] 수용체 하위 단위 3B (Grin3b), 및 글루타메이트 수용체 2 (Gria2)의 발현의 평가에 의해, 상기 조직 및/또는 세포의 조직학, 기능 및 구조를 이해하기 위한 상기 조직 및/또는 세포의 분석 단계를 포함할 수 있다. 일 태양에서, 평가되는 세포는 뉴런, 신경교세포 또는 이들의 조합이다. 다른 태양에서, 평가되는 조직은 뇌 및/또는 척수이다. 상기 제제가 상기 설치류에서, 대조군 설치류와 비교하여, ALS 유사 증상 중 적어도 하나를 억제, 저해, 지연 및/또는 역전시키는지의 여부를 결정하는 방법은 주관적 ALS-TDI 신경학적 점수화, 로타로드 테스트, 캣워크 테스트, 오픈 필드 테스트, 중량 측정, 및/또는 통증 자극에 대한 반응 지체 시간의 결정을 또한 포함할 수 있다.
일부 실시 형태에서, 시험되는 적어도 하나의 증상은 상위 운동 뉴런 기능 장애를 나타내며, 예를 들어 상기 적어도 하나의 증상은 진전, 경직성 마비 (강직), 비정상적인 반사 및 이들의 조합으로 이루어진 군으로부터 선택된다. 일부 실시 형태에서, 시험되는 적어도 하나의 증상은 하위 운동 뉴런 기능 장애를 나타내며, 예를 들어 상기 적어도 하나의 증상은 근육 약화 및 소모, 근섬유다발수축 및 이들의 조합으로 이루어진 군으로부터 선택된다. 일부 실시 형태에서, 평가되는 적어도 하나의 증상은 중량 증가의 감소이다. 일부 실시 형태에서, 시험되는 적어도 하나의 기능은 통각수용의 저하이다.
또한, 운동 뉴런에서 산화 스트레스를 감소시키기 위한 후보 제제를 확인하는 방법으로서, (a) 본 명세서에 개시된 바와 같은 비-인간 동물 모델로부터 유래된 운동 뉴런을 제제의 존재 또는 부재하에 배양하는 단계; (b) 상기 제제가 상기 운동 뉴런에서, 상기 제제의 부재하에 배양된 대조군 운동 뉴런과 비교하여, 산화 스트레스를 억제, 저해 및/또는 감소시키는지의 여부를 결정하는 단계를 포함하며, 상기 운동 뉴런에서의 산화 스트레스의 억제, 저해 및/또는 감소는, 예를 들어, 운동 뉴런에서 산화 스트레스를 감소시키기 위한 후보 제제를 나타내고, ALS의 치료, 억제 및/또는 저해에 유용한 후보 제제일 수 있는 방법이 본 명세서에 제공된다.
일부 실시 형태에서, 상기 후보 제제는 운동 뉴런의 산화 스트레스를 10% 이상, 예를 들어, 15% 이상, 예를 들어, 20% 이상 감소시킨다. 기타 실시 형태에서, 상기 후보 제제의 존재는 운동 뉴런에 의한 산화 스트레스를, 예를 들어, 50% 이상, 예를 들어, 75% 이상, 예를 들어, 80% 이상, 예를 들어, 95% 이상, 예를 들어, 99% 이상 저해한다. 일부 실시 형태에서, 상기 후보 제제는 운동 뉴런에서 산화 스트레스를 억제하며, 예를 들어, 상기 산화 스트레스 수준은 야생형 운동 뉴런의 수준과 유사하다.
또한, (a) 내인성 DR6 신호 펩티드 서열의 내인성 DR6 유전자좌 하류에 삽입하기 위한 벡터를 유도하는, 5' 표적화 아암 및 3' 표적화 아암, (b) 선택 카세트, 및 선택적으로 (c) 막 관통 도메인 인코딩 서열을 포함하는 표적화 벡터가 본 명세서에 제공된다. 일 실시 형태에서, 막 관통 도메인 인코딩 서열은 ROR1 막 관통 도메인을 인코딩한다. 다른 실시 형태에서, 막 관통 도메인 인코딩 서열은 리포터 유전자에 작동가능하게 연결된다. 일 실시 형태에서, 선택 카세트는 리포터 유전자, 약물 내성 유전자 또는 이들의 조합을 포함한다. 다른 실시 형태에서, 선택 카세트는 약물 저항성 유전자를 포함한다. 일부 실시 형태에서, 선택 카세트는 네오마이신 포스포트랜스퍼라제 유전자를 포함한다. 일부 실시 형태에서, 본 명세서에 제공된 바와 같은 표적화 벡터는 서열 번호 16으로 제시된 서열을 포함한다. 일부 실시 형태에서, 표적화 벡터는 선택 카세트를 포함하지 않는다. 일부 실시 형태에서, 본 명세서에 제공된 바와 같은 표적화 벡터는 서열 번호 5로 제시된 서열을 포함한다.
배아 줄기 세포, 예를 들어 쥣과 동물 배아 줄기 세포, 예를 들어 C57BL/6NTac 배아 줄기 세포와 같은 본 명세서에 기재된 바와 같은 표적화 벡터를 포함하는 설치류 세포가 또한 본 명세서에 제공된다.
도 1은 DR6 유전자좌의 표적화된 분열에 대한 전략을 도시한다. 야생형 마우스 DR6 유전자좌가 상단 라인으로 도시되며 (비례척을 사용하지 않음), 5'에서 3' 방향으로 5' UTR (흰색 박스), 개시 코돈 (ATG), 1 내지 6번 엑손 (점 찍힌 박스), 종결 코돈 (TAG) 및 3' UTR이 제시된다. 제시된 바와 같이, 내인성 마우스 신호 서열은 1번 엑손과 2번 엑손의 일부 (개시 코돈으로부터 ~4103번 염기)에 의해 인코딩되고, 신호 펩티드를 인코딩하는 핵산 서열은 하단 라인으로 도시된 (비례척을 사용하지 않음) Zen-Ub1 카세트에 의한 재조합 후 잔존한다. 도 1에 제시된 바와 같이, ZEN-Ub1 표적화 벡터 (서열 번호 5)는 (5'에서 3' 방향으로) 5' 상동성 아암 (5' 아암; 서열 번호 1), ROR1 막 관통 도메인 인코딩 서열 (TM; 서열 번호 7)에 작동가능하게 연결된 이. 콜라이(E. coli) LacZ 리포터 유전자 (LacZ; 서열 번호 8)에 작동가능하게 연결된 폴리아데닐화 신호 (pA; 서열 번호 9)를 포함한다. Zen-Ub1 카세트는 폴리A 신호 (pA; 서열 번호 13)에 작동가능하게 연결된 네오마이신 포스포트랜스퍼라제 저항성 유전자 (Neo; 서열 번호 12)에 작동가능하게 연결된 인간 유비퀴틴 C 유전자 (hUB; 서열 번호 11)의 프로모터를 포함하는 선택 카세트를 또한 포함하며, 상기 선택 카세트는 동일한 loxP 핵산 서열 (서열 번호 10)에 의해 플랭킹되어 있다. Zen-UB1 카세트 (3' 아암)의 3' 상동성 아암은 서열 번호 14로 제시되어 있다. 상동성 재조합 시에, 생성된 대립유전자는 ROR1 막 관통 도메인 인코딩 서열과 LacZ 리포터 유전자에 작동가능하게 연결된 내인성 DR6 신호 서열을 인코딩하는 핵산 서열을 포함하며, 상기 핵산 서열은 서열 번호 17로 제시되고, 2번 엑손의 4103번 염기에서 시작하여 6번 엑손의 종결 코돈까지의 전체 내인성 게놈 서열이 결여되어 있으며, 예를 들어, 내인성 세포질 도메인, 막 관통 도메인 및 세포 외 도메인을 인코딩하는 뉴클레오티드 서열이 결여되어 있다.
도 2a 내지 도 2i는 성별에 관계없이 시간 경과에 따라 빠르게 진행되는 DR6-/- 마우스의 근 위축성 측삭 경화증 (ALS) 유사 운동 손상을 제시한다. 도 2a는 상이한 시점 (x-축)에서의 야생형 (-●-) 및 DR6-/- 마우스 (-■-)의 평균 운동 손상 점수 (y-축)를 제시하는 한편, 도 2b는 상이한 시점 (x-축)에서의 수컷 (-▲-) 및 암컷 (-●-) DR6-/- 마우스의 평균 운동 손상 점수를 제시한다. 도 2c는 상이한 시점 (x-축)에서의 야생형 (-●-) 및 DR6-/- 마우스 (-■-)의 평균 강직 점수 (y-축)를 제시하는 한편, 도 2d는 상이한 시점 (x-축)에서의 수컷 (-▲-) 및 암컷 (-●-) DR6-/- 마우스의 평균 강직 점수를 제시한다. 도 2e는 상이한 시점 (x-축)에서의 야생형 (-●-) 및 DR6-/- 마우스 (-■-)의 평균 진전 점수 (y-축)를 제시하는 한편, 도 2f는 상이한 시점 (x-축)에서의 수컷 (-▲-) 및 암컷 (-●-) DR6-/- 마우스의 평균 진전 점수를 제시한다. 도 2g는 9 내지 21주령 (x-축)에서의 야생형 (-●-) 및 DR6-/- 마우스 (-■-)의 평균 운동 손상 점수 (y-축)를 제시한다. 도 2h는 9 내지 21주령 (x-축)에서의 야생형 (-●-) 및 DR6-/- 마우스 (-■-)의 평균 강직 점수 (y-축)를 제시한다. 도 2i는 9 내지 21주령 (x-축)에서의 야생형 (-●-) 및 DR6-/- 마우스 (-■-)의 평균 진전 점수를 제시한다. 실험 1 (도 2a 내지 도 2f)에서, 야생형 마우스는 n=12이고, DR6-/- 마우스는 n=16이고 (수컷 DR6-/- 마우스 n=8, 암컷 DR6-/- 마우스 n=8), 각 시점에서 야생형 동물은 각각의 시험에 대해 0점이었다. 실험 2 (도 2g 내지 도 2i)에서, 야생형 마우스는 n=14이고, DR6-/- 마우스는 n=18이다. 모든 데이터는 평균 ± SEM으로 보고되어 있다. 통계 분석을 위해 양방향 ANOVA를 수행하여 야생형 마우스의 값을 녹아웃 마우스의 값과 비교하며, *는 P ≤ 0.05, **는 P ≤ 0.01, ***은 P ≤ 0.001, 및 ****는 P ≤ 0.0001이다.
도 3a 내지 도 3f는, 중량 감소 및 유의한 운동 이상을 나타낸 DR6-/- 마우스의 ALS 유사 표현형을 제시한다. 도 3a: 좌측 및 우측 패널은 각각 2014년 5월 29일과 2014년 6월 11일의 야생형 (tnsfr21+/+) 및 DR6-/- (tnsfr21-/-) 마우스의 체중 (y-축; 그램)을 제시한다. 도 3b: 좌측 및 우측 패널은 각각 야생형 (tnsfr21+/+; 줄무늬 막대) 및 DR6-/- (tnsfr21-/-; 단색 막대) 마우스의 로타로드에서 추락할 때까지의 최대 지체 시간과 중위 지체 시간 (y-축; 초)을 제시한다. 도 3c: 60분 동안 야생형 (tnsfr21+/+; 줄무늬 막대) 및 DR6-/- (tnsfr21-/-; 단색 막대) 마우스의 총 부동 상태 (y-축), 기본 이동 (y-축) 및 미세 운동 이동이 제시되어 있다. 도 3d: 야생형 (tnsfr21+/+; 줄무늬 막대) 및 DR6-/- (tnsfr21-/-; 단색 막대) 마우스의 1시간 동안의 X+Y 보행 (y-축)이 제시되어 있다. 도 3e: 좌측 및 우측 패널은 각각 야생형 (tnsfr21+/+; 줄무늬 막대) 및 DR6-/- (tnsfr21-/-; 줄무늬 막대)의 양육 합계 (y-축) 및 총 양육 시간 (y-축; 초)을 제시한다. 도 3f: 좌측 및 우측 패널은 각각 야생형 (tnsfr21+/+; 줄무늬 막대) 및 DR6-/- (tnsfr21-/-; 단색 막대)의 총 이동 거리 및 총 휴식 시간 (y-축; 60분의 합계)을 제시한다. * (p <0.05)는 두 그룹 사이에 통계적으로 유의한 차이를 나타낸다.
도 4a 내지 도 4c는 9 내지 21주령의 야생형 (-●-; n=14) 및 DR6-/- (-■-; n=18) 마우스 (x-축)의 (A) 평균 체중 (y-축; 그램 단위 중량) (B) 로타로드에서 추락할 때까지의 중위 지체 시간 (y-축; 초) 및 (C) 로타로드에서 추락할 때까지의 최대 시간 (y-축; 초)을 제시한다. 로타로드에서 소요된 최대 시간은 180초였다. 모든 데이터는 평균 ± SEM으로 보고되어 있다. 통계 분석을 위해 양방향 ANOVA를 수행하여 야생형 마우스의 값을 녹아웃 마우스의 값과 비교하며, *는 P ≤ 0.05, **는 P ≤ 0.01, ***은 P ≤ 0.001, 및 ****는 P ≤ 0.0001이다.
도 5a 내지 도 5h는 야생형 및 DR6-/- 마우스의 오픈 필드 보행성 운동 거동, 예를 들어, 10 내지 21주령의 야생형 (-●-; n=14) 및 DR6-/- (녹아웃; -■-; n =16) 동물의 1시간 동안의 총 거리 (도 5a), 부동 상태 (도 5b), 총 양육 시간 (도 5c), 기본 이동 (도 5d), 미세 이동 (도 5e), X+Y 보행 (도 5f), 총 휴식 (도 5g) 및 양육 (도 5h)을 제시한다. 모든 데이터는 평균 ± SEM으로 보고되어 있다. 통계 분석을 위해 양방향 ANOVA를 수행하여 야생형 마우스의 값을 녹아웃 마우스의 값과 비교하며, *는 P ≤ 0.05, **는 P ≤ 0.01, ***은 P ≤ 0.001, 및 ****는 P ≤ 0.0001이다.
도 6a 내지 도 6f는 10 내지 21주령의 야생형 (-●-; n=14) 및 DR6-/- (녹아웃; -■- n=18) 동물의 캣워크 거동, 예를 들어, 하지간 협응 (interlimb coordination) (도 6a), 발 압력 (도 6b), 발자국 면적 (도 6c), 보폭 길이 (도 6d), 입각기 (stance phase) (도 6e), 유각기 (swing phase) (도 6f), 유각 속도 (swing speed) (도 6g), 및 듀티 사이클 (도 6h)을 제시한다. 모든 데이터는 평균 ± SEM으로 보고되어 있다. 통계 분석을 위해 양방향 ANOVA를 수행하여 야생형 마우스의 값을 녹아웃 마우스의 값과 비교하며, *는 P ≤ 0.05, **는 P ≤ 0.01, ***은 P ≤ 0.001, 및 ****는 P ≤ 0.0001이다.
도 7a 내지 도 7g는 9 내지 21주령의 야생형 (-●-, n=14) 및 DR6-/- (-■-; n=18) 동물 (x 축)의 앞다리와 뒷다리의 걸음걸이 능력 (gait ability)을 제시한다. 도 7a는 평균 앞발 압력 및 평균 뒷발 압력을 제공한다. 도 7b는 평균 앞발자국 면적 및 평균 뒷발자국 면적을 제공한다. 도 7c는 평균 앞다리 보폭 길이 및 평균 뒷다리 보폭 길이를 제공한다. 도 7d는 평균 앞다리 입각기 및 평균 뒷다리 입각기를 제공한다. 도 7e는 평균 앞다리 유각 속도 및 평균 뒷다리 유각 속도를 제공한다. 도 7f는 평균 앞다리 유각기 및 평균 뒷다리 유각기를 제공한다. 도 7g는 평균 앞다리 듀티 사이클 및 평균 뒷다리 듀티 사이클을 제공한다. 모든 데이터는 평균 ± SEM으로 보고되어 있다. 통계 분석을 위해 양방향 ANOVA를 수행하여 야생형 마우스의 값을 녹아웃 마우스의 값과 비교하며, *는 P ≤ 0.05, **는 P ≤ 0.01, ***은 P ≤ 0.001, 및 ****는 P ≤ 0.0001이다.
도 8은 10 내지 21주령의 야생형 (-●-) 및 DR6-/- (녹아웃; -■-) 동물의 신경학적 점수, 예를 들어, 운동 손상 점수, 진전 점수 및 강직 점수를 제시한다. 모든 데이터는 평균 ± SEM으로 보고되어 있다. 통계 분석을 위해 양방향 ANOVA를 수행하여 야생형 마우스의 값을 녹아웃 마우스의 값과 비교하며, *는 P ≤ 0.05, **는 P ≤ 0.01, ***은 P ≤ 0.001, 및 ****는 P ≤ 0.0001이다.
도 9는 야생형 (좌측) 또는 DR6-/- (우측) 동물로부터 단리되고 헤마톡실린 및 에오신에 의해 면역염색된 뇌의 대표 슬라이드를 상단 패널에 제시한다. 또한, 하단 패널에는 야생형 (좌측 막대) 또는 DR6-/- (녹아웃, 우측 막대) 동물의 척수의 운동 뉴런의 수 (y-축; 평균 운동 뉴런 수)가 제시되어 있다.
도 10은 야생형 (좌측 막대) 또는 DR6-/- (녹아웃; 우측 막대) 동물의 척수 (좌측 패널)와 뇌 (우측 패널)의 MBP (상단 패널) 또는 NGFR (하단 패널)의 mRNA의 수준 (β-액틴에 대비한 평균 mRNA 수준; y-축)을 제시한다.
도 11은 야생형 (좌측 막대) 또는 DR6-/- (녹아웃; 우측 막대) 동물의 척수 (좌측 패널)와 뇌 (우측 패널)의 Chat (상단 패널) 또는 Mnx (하단 패널)의 mRNA의 수준 (β-액틴에 대비한 평균 mRNA 수준; y-축)을 제시한다.
도 12는 야생형 (좌측 막대) 또는 DR6-/- (녹아웃; 우측 막대) 동물의 척수 (좌측 패널)와 뇌 (우측 패널)의 Grin3b (상단 패널) 또는 Gria2 (하단 패널)의 mRNA의 수준 (β-액틴에 대비한 평균 mRNA 수준; y-축)을 제시한다.
도 13은 야생형 (WT; x-축) 또는 DR6-/- (KO; x-축) 마우스의 흉선, 비장 또는 말초로부터 수득된 모 세포의 퍼센트 (모 %; y-축)로서 상이한 세포들의 수를 제시한다.
도 14는 야생형 (WT; x-축) 또는 DR6-/- (KO; x-축) 마우스의 흉선, 비장 또는 말초로부터 수득된 모 세포의 퍼센트 (모 %; y-축)로서 상이한 세포들의 수를 제시한다.
도 15는 야생형 (n=6, 흑색 막대) 또는 DR6-/- (n=6, 회색 막대) 동물의 혈청에서의 상이한 사이토카인 또는 케모카인 (x-축)의 농도 (평균 관찰 농도; y-축)를 제시한다.
도 16a는 야생형 동물 (DR6+/+, 흑색 막대, 축)과 DR6+/- 동물 (DR6+/-; 바둑판 무늬 막대; x-축)로부터 수득된 배아 줄기 세포 유래 운동 뉴런의 배양 후 수득된 생존 세포의 수 (y-축)를 제시한다. 도 16b는 배아 줄기 세포 유래 운동 뉴런 (ESMN) 배지에서, 1일 또는 7일째 (x-축)에 야생형 (DR6+/+, 흑색 막대)과 이형접합 동물 (DR6+/-; 바둑판 무늬 막대)로부터 수득된 배아 줄기 세포 유래 운동 뉴런의 산화 스트레스 (평균 광학 밀도; y-축)를 제시한다. ****는 P≤ 0.0001이다.
도 17a는 10 내지 21주령의 야생형 (-●-, n=27), 이형접합 DR6+/- (-■-; n=14), 및 동형접합 DR6-/- (-▲-; n=33) 동물의 신경학적 점수, 예를 들어, 운동 손상 점수, 진전 점수 및 강직 점수를 제시한다. 도 17b는 10 내지 21주령의 야생형 (-●-, n=27), 이형접합 DR6+/- (-■-; n=14), 및 동형접합 DR6-/- (-▲-; n=33) 동물의 평균 체중 (y-축; 그램 단위 중량)을 제시한다. 도 17c의 상단 패널은 야생형 (-●-; n=13), 이형접합 DR6+/- (-■-; n=14), 및 동형접합 DR6-/- (-▲-; n=15) 동물의 로타로드에서 추락할 때까지의 중위 지체 시간 (y-축; 초)을 제시하며; 하단 패널은 12 내지 21주령의 야생형 (-●-; n=13), 이형접합 DR6+/- (-■-; n=14), 및 동형접합 DR6-/- (-▲-; n=15) 동물의 로타로드에서 추락할 때까지의 최대 시간 (y-축; 초)을 제시한다. 로타로드에서 소요된 최대 시간은 180초였다. 도 17d 내지 도 17k는 야생형, 이형접합 DR6+/-, 및 동형접합 DR6-/- 마우스의 오픈 필드 보행성 운동 거동, 예를 들어 10 내지 21주령의 야생형 (-●-; n=27), 이형접합 DR6+/- (-■-; n=14), 및 동형접합 DR6-/- (-▲-; n=33) 동물의 1시간 동안의 양육 (도 17d), 총 양육 시간 (도 17e), 기본 이동 (도 17f), 부동 상태 (도 17g), 미세 이동 (도 17h), X+Y 보행 (도 17i), 총 거리 (도 17j) 및 총 휴식 (도 17k)을 제시한다. 도 17l 내지 도 17z는 10 내지 21주령의 야생형 (-●-; n=27), 이형접합 DR6+/- (-■-; n=14), 및 동형접합 DR6-/- (-▲-; n=33) 동물의 캣워크 거동, 예를 들어 보폭 길이 (도 17l 및 도 17m), 유각 속도 (도 17n 및 도 17o), 하지간 협응 (도 17p), 유각기 (도 17q 및 도 17r), 듀티 사이클 (도 17s 및 도 17t), 발자국 면적 (도 17u 및 도 17v), 입각기 (도 17w 및 도 17x), 및 발 압력 (도 17y 및 도 17z)을 제시한다. 모든 데이터는 평균 ± SEM으로 보고되어 있다. 통계 분석을 위해 양방향 ANOVA를 수행하여 야생형 마우스의 값을 녹아웃 마우스의 값과 비교하며, *는 P ≤ 0.05, **는 P ≤ 0.01, ***은 P ≤ 0.001, 및 ****는 P ≤ 0.0001이다.
도 18은 48℃, 52℃, 또는 55℃의 일정한 온도로 가온된 금속 판에 적용된 야생형 (좌측 막대; n=13), 이형접합 DR6+/- (중간 막대; n=14), 및 동형접합 DR6-/- (우측 막대; n=15) 동물의 통각수용 반응에 대한 지체 시간 (시간; y-축)을 제시한다. 모든 데이터는 평균 ± SEM으로 보고되어 있다. 통계 분석을 위해 양방향 ANOVA를 수행하여 야생형 마우스의 값을 녹아웃 마우스의 값과 비교하며, *는 P ≤ 0.05, **는 P ≤ 0.01, ***은 P ≤ 0.001, 및 ****는 P ≤ 0.0001이다.
루게릭병이라고도 부르는 근 위축성 측삭 경화증 ("ALS")은 2만 명에 달하는 미국인이 앓고 있으며, 미국에서 매년 5,000 건의 신규 사례가 발생하는 치명적인 신경학적 진행성 질환이다. 상기 장애는 운동 뉴런 질환으로 알려진 장애의 한 부류에 속한다. ALS는 수의 운동을 제어하는 뇌와 척수의 특정 신경 세포가 점차적으로 퇴화될 때 발생한다. 뇌와 척수 둘 모두가 신체의 근육에 메시지를 개시 및 전달하는 능력을 상실한다. 기능할 수 없는 근육은 점차적으로 위축되고 단수축 (twitch)된다.
ALS는 근육이 먼저 약화됨에 따라 다른 방식으로 자체적으로 나타난다. 증상은 엎어지기(tripping)와 넘어지기(falling), 손과 팔의 운동 제어 상실, 말하기, 연하 및/또는 호흡 곤란, 지속적인 피로감, 및 단수축과 경련을 포함할 수 있으며, 때때로 매우 중증의 증상으로 나타난다. 결국, 횡격막과 흉부 벽의 근육이 너무 약해지면, 환자는 호흡을 위해 인공 호흡기를 필요로 하게 된다. ALS를 앓고 있는 대부분의 사람들은 보통 진단 후 3년 내지 5년 후 호흡 부전으로 사망하나; 일부 사람들은 진단 후 10년 이상 생존한다. ALS는 중년기에 발생한다. 남성이 여성보다 상기 질환에 걸릴 가능성이 약 1.5배 높다.
ALS에 대한 완치 사례는 없으며, 상기 장애의 진행을 억제하거나 역전시키는 입증된 치료법도 존재하지 않는다. 미국 식품의약국 (FDA)은 최근 ALS 환자의 생존 기간을 연장시키는 것으로 나타난 최초의 약물인 릴루졸 (riluzole)을 승인하였다. 환자는 또한 그 증상의 일부를 다루는 지지적 치료를 받을 수 있다.
인간에서 ALS를 치료하는 데 유용할 수 있는 후보 제제를 찾는 데 유용할 수 있는 ALS에 대한 동물 모델이 본 명세서에 제공된다.
본 명세서에 기재되거나 참조된 기술 및 절차는 당업자에 의해 일반적으로 잘 이해되며, 예를 들어, 문헌 [Sambrook et al., Molecular Cloning: A Laboratory Manual 2nd. edition (1989) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.]에 기재된 광범위하게 사용되는 분자 클로닝 방법론과 같은 종래의 방법론을 사용하여 보통 사용된다. 적절하게, 구매가능한 키트 및 시약의 사용을 포함하는 절차는, 달리 명시되지 않는 한, 일반적으로 제조업체에 의해 규정된 프로토콜 및/또는 파라미터에 따라 수행된다.
본 방법 및 검정에 대해 기재하기 전에, 본 발명은 기재된 특정 방법론, 프로토콜, 세포주, 동물 종 또는 속, 작제물 및 시약에 제한되지 않으며, 따라서 물론 변화될 수 있음이 이해되어야 한다. 단수 형태("a", "an" 및 "the")는, 문맥이 명확하게 달리 지시하지 않는 한, 복수의 지시 대상을 포함한다는 것을 알아야 한다. 따라서, 예를 들어, "유전자 변이"에 대한 언급은 복수의 이러한 변이를 포함하며, "프로브"에 대한 언급은 당업자에 공지된 하나 이상의 프로브 및 그 균등물 등에 대한 언급을 포함한다. 본 명세서 및 관련된 청구 범위에 인용된 모든 숫자 (예를 들어, 아미노산 22 내지 81, 1 내지 354 등)는 용어 "약"에 의해 수식되는 것으로 이해된다.
본 명세서에 언급된 모든 간행물은, 인용된 간행물과 연계된 방법 및/또는 재료를 개시하고 기재하기 위해 본 명세서에 참고로 포함된다. 본 명세서에 인용된 간행물들은 본 출원의 출원일 이전의 그들의 개시에 대해 인용된다. 여기서, 어떠한 것도, 더 빠른 발명 우선일 또는 선행일로 인해, 본 발명자들이 상기 간행물들에 대해 선행 권리가 없다는 것을 인정하는 것으로 해석되어서는 안 된다. 또한, 실제 공개일은 표시된 것과 상이할 수 있으며, 별도의 확인이 필요할 수 있다.
정의
용어 "배아 줄기 세포" 또는 "ES 세포"는 배아로의 도입 시에 발생 중인 배아의 임의의 조직에 기여할 수 있는 배아 유래 전능성 또는 만능성 세포를 포함한다. 용어 "만능성 세포"는 하나 초과의 분화된 세포 유형으로 발생할 능력을 갖는 미분화된 세포를 포함한다.
일부 표적화 벡터는 "대형 표적화 벡터" 또는 "LTVEC"이며, 이는 진핵 세포에서 상동성 유전자 표적화를 수행하도록 의도된 다른 접근법에 의해 전형적으로 사용되는 것보다 큰 핵산 서열에 상응하며 이로부터 유래되는 상동성 아암을 포함하는 진핵 세포에 대한 대형 표적화 벡터를 포함한다. LTVEC의 예는 박테리아 상동 염색체 (BAC) 및 효모 인공 염색체 (YAC)를 포함하지만, 이에 한정되지 않는다. LTVEC를 사용하여 표적화된 유전자 변형을 생성하는 예는, 예를 들어, 각각 모든 목적을 위하여 본 명세서에 전체적으로 참고로 포함된, 국제 특허 공개 WO 2015/088643호, 미국 특허 출원 공개 제2015/0159175호, 미국 특허 출원 공개 제2015/0159174호, 미국 특허 출원 공개 제2014/0310828호, 미국 특허 출원 공개 제2014/0309487호 및 미국 특허 출원 공개 제2013-0309670호에 개시되어 있다. LTVEC는 세포에서 상동성 재조합을 수행하도록 의도된 다른 접근법에 의해 전형적으로 사용되는 것보다 큰 핵산 서열을 갖는 핵산 삽입물을 포함하는 표적화 벡터를 또한 포함한다. 예를 들어, LTVEC는, 크기 제한 때문에 전통적인 플라스미드 기반 표적화 벡터에 의해 수용될 수 없는 큰 유전자좌의 변형을 가능하게 한다. 예를 들어, 표적화된 유전자좌는, 뉴클레아제 제제 (예를 들어, Cas 단백질)에 의해 유도된 닉 또는 이중-가닥 브레이크의 부재하에서 단지 부정확하게 또는 단지 유의하게 낮은 효율로 표적화될 수 있거나 종래의 방법을 사용하여 표적화될 수 없는 세포의 유전자좌일 수 있다 (즉, 5' 및 3' 상동성 아암은 그러한 유전자좌에 상응할 수 있다).
LTVEC의 예는 박테리아 인공 염색체 (BAC), 인간 인공 염색체 또는 효모 인공 염색체 (YAC)로부터 유래된 벡터를 포함한다. LTVEC의 비제한적인 예 및 이들을 제조하는 방법은 예를 들어, 각각 본 명세서에 참고로 포함된, 미국 특허 제6,586,251호; 미국 특허 제6,596,541호; 미국 특허 제7,105,348호; 및 국제 특허 공개 WO 2002/036789호 (PCT/US01/45375호)에 기재되어 있다. LTVEC는 선형 형태 또는 원형 형태일 수 있다.
LTVEC는 예를 들어, 10 kb 이상 또는 약 50 kb 내지 약 400 kb 이상을 포함하는 임의의 길이일 수 있다. LTVEC의 크기는 너무 커서 종래의 검정, 예를 들어 서던 블롯팅(southern blotting) 및 긴 범위(long-range) (예를 들어, 1 kb 내지 5 kb) PCR에 의한 표적화 사건의 스크리닝을 가능하게 할 수 없다. 5' 상동성 아암 및 3' 상동성 아암의 총합은, 예를 들어, 10 kb 이상일 수 있다 (각 상동성 아암은, 예를 들어 약 5 kb 내지 약 200 kb의 범위일 수 있다). LTVEC 및 핵산 삽입물은, 예를 들어 약 5 kb 내지 약 3 Mb (예를 들어, 약 500 kb 이상)의 길이의 표적 유전자좌에서의 결실이 허용되도록 설계될 수 있다. 마찬가지로, LTVEC 및 핵산 삽입물은, 예를 들어 약 5 kb 내지 약 400 kb 이상의 범위의 길이의 외인성 핵산 서열의 표적 유전자좌 내로의 삽입이 허용되도록 설계될 수 있다.
용어 "재조합 부위"는, 부위-특이적 재조합효소에 의해 인식되고 재조합 사건에 대한 기질의 역할을 할 수 있는 뉴클레오티드 서열을 포함한다.
용어 "부위-특이적 재조합효소"는 "재조합 부위들" 사이의 재조합을 촉진할 수 있는 효소 군을 포함한다. "부위-특이적 재조합효소"의 예는 Cre, Flp, 및 Dre 재조합효소를 포함하지만 이에 한정되지 않는다.
핵산 서열과 관련하여 용어 "생식세포계열(germline)"은 자손으로 전해질 수 있는 있는 핵산 서열을 포함한다.
어구 "작동가능하게 연결된"은 성분들이 그들의 의도된 방식으로 함께 기능하도록 연결되어 있음을 의미한다. 한 경우에, 단백질을 인코딩하는 핵산 서열이 적당한 전사 조절을 보유하도록 조절 서열 (예를 들어, 프로모터, 인핸서, 사일렌서(silencer) 서열 등)에 작동가능하게 연결될 수 있다. 다른 경우에, 신호 펩티드를 인코딩하는 핵산 서열은, 예를 들어 막 관통 도메인을 인코딩하는 핵산 서열에 작동가능하게 연결되어 있어, 상기 막 관통 도메인에 작동가능하게 융합된 신호 펩티드를 포함하는 폴리펩티드로 번역될 수 있으며, 신호 펩티드와 막 관통 도메인 둘 모두는 그들의 각각의 생물학적 기능을 보유한다.
용어 "유전자좌"는 게놈 DNA 내의 DNA의 분절로 정의된다. 예를 들어, DR6 유전자좌는 DR6을 인코딩하는 게놈 DNA 내의 DNA의 분절이며, DR6의 발현에 관여하는 비번역 및/또는 조절 DNA를 포함한다.
사멸 수용체 6 (DR6) (문헌에서 "TR9"로 또한 지칭되며; 문헌에서 TNF 수용체 슈퍼패밀리 멤버 21 또는 TNFRS21로 또한 알려져 있다)으로 불리는 TNFR 패밀리 멤버는 4개의 세포 외 시스테인-풍부 모티프 및 세포질 사멸 도메인 구조를 가지는 I 형 막 관통 수용체로 기재되어 왔다 (문헌[Pan et al., FEBS Lett., 431:351-356 (1998)]; 또한 미국 특허 제 6,358,508호; 제 6,667,390호; 제 6,919,078호; 제 6,949,358호 참조). 특정 형질감염 세포주에서 DR6의 과발현은 NF-kB와 JNK 둘 모두의 활성화 및 세포 사멸을 야기하는 것으로 보고되어 있다 (문헌[Pan et al., FEBS Letter, 431:351-356 (1998)]). DR6 결손 마우스 모델에서, T 세포가 JNK 활성화 시에 실질적으로 손상되었으며, DR6 (-/-) 마우스를 단백질 항원으로 챌린징한 경우, 그의 T 세포가 과증식하여, Th2 반응에 대해 온전한 편향성을 나타내는 것으로 밝혀졌다 (반면, Th1 분화는 동일한 영향을 받지 않았다) (문헌[Zhao et al., J. Exp. Med., 194:1441-1448 (2001)]). 또한, 시험관 내에서, DR6의 표적화된 분열이 T 헬퍼 2 (Th2) 분화의 향상을 야기하는 것으로 보고되었다 (상기 Zhao 등의 문헌). B 세포 매개 병증 또는 신경 병증의 조절에서 DR6 작동제 또는 길항제의 다양한 용도가 2005년 3월 31일자로 공개된 미국 특허 출원 공개 제2005/0069540호 및 2010년 8월 12일자로 공개된 미국 특허 출원 공개 제2010/0203044호에 기재되었다.
쥣과 동물의 DR6 수용체 유전자좌는 17번 염색체에서 발견되며, 6번 엑손을 가진다. 이는 추정 신호 서열 (아미노산 1 내지 41), 세포 외 도메인 (아미노산 42 내지 349), 막 관통 도메인 (아미노산 350 내지 370), 이어서, 세포질 도메인 (아미노산 370 내지 655)을 가지는 655개의 아미노산 단백질 (서열 번호 16)을 인코딩한다. 성숙 DR6 단백질은 그의 신호 단백질의 절단 후 번역된 폴리펩티드를 지칭하며, 예를 들어, 성숙한 쥣과 동물의 DR6 단백질은 서열 번호 16의 아미노산 42 내지 655를 지칭한다.
서열 번호에 의해 확인되는 서열에 대한 간단한 설명이 하기 표 1에 제공된다.
[표 2]
Figure pat00001
I. 적어도 하나의 DR6 유전자좌의 유전자 변형을 포함하는 조성
내인성 DR6 세포질 도메인의 임의의 일부분을 인코딩하는 뉴클레오티드 서열이 결여되고, DR6 신호 펩티드, 막 관통 도메인 및/또는 리포터 단백질을 인코딩하는 핵산 서열을 추가로 포함할 수 있는 변형 DR6 유전자좌를 포함하는 비-인간 동물, 세포, 조직 및 배아가 제공된다. DR6 발현을 조작하기 위한 방법 및 조성이 제공된다. DR6의 변형을 유도하는 표적화 조성이 또한 제공된다. DR6 기능의 조절과 관련된 운동 뉴런 기능 장애, 예를 들어, ALS 유사 표현형을 나타내는 비-인간 동물, 세포 및 조직이 제공된다. 하기 설명은 구체적인 특정 DR6의 조사된 사항에 관련된 것이나, 상기 방법 및 조성은 임의의 DR6을 사용하여 실행될 수 있다.
DR6 유전자좌의 표적화된 유전자 변형 (예를 들어, 리포터 녹인(knock-in))을 위해 사용될 수 있거나, DR6 기능에 영향을 줄 수 있는 변형 DR6 유전자좌 및/또는 핵산을 포함하는 비-인간 동물, 세포, 조직 및 배아가 본 명세서에 제공된다. 이러한 경우, 상기 변형 DR6 유전자좌는 DR6을 인코딩하는 핵산 서열에서의 기능 상실 돌연변이를 포함한다. 또한, DR6의 기능 상실 돌연변이를 포함하는 비-인간 동물 유래의 세포, 조직 및 배아가 제공된다.
DR6과 관련된 용어 "기능 상실"은 DR6의 발현의 감소 또는 결여 및/또는 DR6의 활성/기능의 감소 또는 결여를 야기하는 DR6 유전자좌의 임의의 변형 및/또는 트랜스진의 발현을 포함할 수 있다. DR6의 발현 수준은, 예를 들어, 세포 또는 유기체에서 DR6의 수준을 검정함으로써, 직접 측정할 수 있다.
일반적으로, DR6 발현 수준 및/또는 DR6의 활성 수준이, DR6의 발현 및/또는 활성이 저해되도록 유전자 변형되거나 돌연변이가 유발되지 않은 적절한 대조군 세포 또는 유기체의 DR6 수준보다 통계적으로 낮은 경우 (p≤0.05), DR6의 발현 수준 및/또는 DR6의 활성이 저하된다. 구체적인 실시 형태에서, DR6의 농도 및/또는 활성은 DR6의 수준 및/또는 활성을 저하시키도록 변형되지 않은 대조군 세포 또는 유기체에 비해, 적어도 1%, 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 99% 또는 그 이상 저하된다.
다른 경우에는, DR6의 발현 수준 및/또는 활성을 저하시키는 표적화된 유전자 변형을 갖는 세포 또는 유기체는 서던 블롯 분석(Southern blot analysis), DNA 염기 서열 결정(sequencing), PCR 분석, 또는 표현형 분석을 포함하지만 이에 한정되지 않는 방법을 사용하여 선택된다. 이어서, 이러한 세포 또는 유기체는 본 명세서에 기재된 다양한 방법 및 조성에 이용된다.
"대상 세포" 또는 "대상 유기체"는 유전자 변이, 예컨대 본 명세서에 개시된 유전자 변형을 일으킨 것이거나, 이렇게 변이된 세포/유기체의 자손이며 상기 변이를 포함하는 세포/유기체이다. "대조군" 또는 "대조군 세포" 또는 "대조군 유기체"는 대상 세포 또는 유기체의 표현형 변화를 측정하는 기준점을 제공한다. 일 실시 형태에서, 대조군 세포/유기체는, 발현 및/또는 활성의 저하를 야기하는 유전자 변형 또는 돌연변이가 결여된 것을 제외하고는, DR6에서 유전자 변형을 갖는 세포/유기체와 가능한 한 거의 일치한다 (예를 들어, 각 세포는 동일한 세포주로부터 유래할 수 있음). 다른 예에서, 대조군 세포/유기체는, 예를 들어: (a) 야생형 세포/유기체, 즉, 대상 세포/유기체를 초래하는 유전자 변이를 위한 출발 물질과 동일한 유전자형의 세포/유기체; (b) 널 작제물 (null construct) (즉, 마커 유전자를 포함하는 작제물과 같은, 관심 특질에 대한 알려진 효과가 없는 작제물)에 의해 유전자 변형된 것을 제외하고는, 출발 물질과 동일한 유전자형의 세포/유기체; (c) 대상 세포/유기체의 비-유전자 변형 자손인 세포/유기체 (즉, 대조군 세포 및 대상 세포가 동일 세포주로부터 유래됨); (d) 대상 세포/유기체와 유전적으로 동일하나, 관심 유전자의 발현을 유도하는 조건 또는 자극에 노출되지 않은 세포/유기체; 또는, (e) 유전자 변형이 관심 폴리뉴클레오티드의 발현에 변이를 초래하지 않는 조건 하의 대상 세포/유기체 그 자체를 포함할 수 있다.
동물, 세포, 조직 또는 배아와 관련하여 용어 "동물"은 포유류, 어류 및 조류를 포함한다. 포유류는, 예를 들어, 인간, 비-인간 영장류, 원숭이, 유인원, 고양이, 개, 말, 황소, 사슴, 들소, 양, 설치류 (예를 들어, 마우스, 래트, 햄스터, 기니피그), 가축 (예를 들어, 소 종(bovine species), 예를 들어, 암소, 거세 황소 등; 양 종(ovine species), 예를 들어, 양, 염소 등; 및 돼지 종, 예를 들어, 피그 및 수퇘지)을 포함한다. 조류는 예를 들어, 닭, 칠면조, 타조, 거위, 오리 등을 포함한다. 사육된 동물 및 농업용 동물이 또한 포함된다. 동물, 세포, 조직, 또는 배아와 관련하여, 어구 "비-인간 동물"은 인간을 제외한다.
일 실시 형태에서, 동물은 비-인간 동물이다. 다른 실시 형태에서, 비-인간 동물은 포유류이다. 다른 실시 형태에서, 포유류는 설치류이다. 추가 실시 형태에서, 설치류는 마우스, 래트 또는 햄스터이다. 추가 실시 형태에서, 설치류는 마우스 또는 래트이다. 일부 실시 형태에서, 설치류는 마우스이다.
본 명세서에 기재된 바와 같은 유전자 변형은 DR6 유전자좌로부터의 결실, DR6 유전자좌로의 첨가, DR6 유전자좌 또는 그 일부분의 대체 및/또는 이들의 임의의 조합 중 하나 이상을 포함할 수 있다. 유전자좌는 코딩 영역 또는 비-코딩 조절 영역을 포함할 수 있다. 본 명세서에 기재된 바와 같은 유전자 변형은 또한 DR6 유전자좌 외부의 게놈에서의 트랜스진의 삽입을 포함할 수 있으며, 상기 트랜스진의 발현은 리간드 결합, 세포막 내의 배치 등에 대해 내인성 DR6 단백질을 방해하고, 예를 들어 그와 경쟁한다.
본 명세서에 제공된 유전자 변형은 DR6 유전자좌로 표적화될 수 있다. DR6의 기능 상실은 DR6 유전자의 표적화된 유전자 변형 (즉, 조절 영역, 코딩 영역, 엑손 및/또는 인트론 등에서의 유전자 변형)으로부터 야기될 수 있다. 이러한 표적화된 변형은 하나 이상의 뉴클레오티드의 첨가, 하나 이상의 뉴클레오티드의 결실, 하나 이상의 뉴클레오티드의 치환, DR6 유전자좌의 분열, DR6 유전자좌 또는 그 일부분의 녹아웃, DR6 유전자좌 또는 그 일부분의 녹인, 내인성 DR6 핵산 서열 또는 그 일부분의 이종성 핵산 서열로의 대체, 또는 이들의 조합을 포함하지만 이에 한정되지 않는다. 구체적인 실시 형태에서, 적어도 1, 2, 3, 4, 5, 7, 8, 9, 10, 50, 100, 400개 또는 그 이상의 뉴클레오티드가 변화되어 표적화된 게놈 변형을 형성한다.
일 실시 형태에서, 기능 상실 돌연변이는 적어도 하나의 DR6 기능의 분열 또는 녹아웃을 특징으로 한다.
DR6 유전자좌는 상기 유전자좌의 임의의 영역에서 유전자 변형되어, 변형에 의해 DR6 기능의 조절이 야기되도록 할 수 있다. 일 실시 형태에서, DR6 유전자좌의 변형은 전체 DR6 코딩 영역 또는 그 일부분의 결실을 포함한다. 일 실시 형태에서, 변형 DR6 유전자좌는 성숙 DR6 단백질 또는 그 일부분을 인코딩하는 하나 이상의 엑손의 결실을 포함한다. 다른 실시 형태에서, 상기 결실은 DR6 유전자좌의 제1, 제2, 제3, 제4, 제5 및/또는 제6 엑손에서 개시되는 DR6 유전자좌 내의 하나 이상의 엑손의 결실을 포함한다. 다른 실시 형태에서, 상기 결실은 DR6 유전자좌의 제2 엑손에서 개시되는 DR6 유전자좌 내의 하나 이상의 엑손의 결실을 포함한다.
일부의 경우, DR6 유전자좌 또는 그 일부분은 삽입 핵산으로 대체된다. 이러한 경우, 상기 대체는 DR6 유전자좌 또는 그 일부분의 전체 RNA 코딩 영역의 삽입 핵산으로의 대체, DR6 유전자좌의 하나 이상의 엑손의 삽입 핵산으로의 대체, DR6 유전자좌의 제1 엑손에서 개시되는 DR6 유전자좌 내의 하나 이상의 엑손의 삽입 핵산으로의 대체 또는 제2 엑손에서 개시되는 DR6 유전자좌 내의 하나 이상의 엑손의 삽입 핵산으로의 대체일 수 있다.
일부 예에서, 삽입 핵산은, 내인성 DR6 프로모터와 작동가능하게 연결되어 내인성 DR6 프로모터가 삽입 핵산의 발현을 유도하도록 DR6 유전자좌에 위치한다. 이러한 경우, 상기 핵산 서열의 발현은 DR6의 발현 패턴을 따른다.
일 실시 형태에서, DR6 유전자좌 또는 그 일부분은, 리포터를 인코딩하는 제1 핵산 서열을 포함하는 삽입 핵산으로 대체된다. 예를 들어, 상기 삽입 핵산이 리포터 유전자를 포함하고, DR6 프로모터에 작동가능하게 연결되어 DR6 유전자좌 내에 위치하는 경우, 리포터 유전자의 발현은 내인성 DR6 프로모터에 의해 유도된다. 대안적으로, 상기 삽입 핵산은 내인성 DR6 프로모터와 작동가능하게 연결되어 삽입되지 않는다. 이러한 경우, 상기 삽입 핵산은 프로모터를 포함할 수 있다. 일 실시 형태에서, 삽입 핵산은, 리포터 유전자의 발현을 유도하는 프로모터에 작동가능하게 연결된 리포터 유전자를 포함한다.
일부 실시 형태에서, 삽입 핵산은 내인성 DR6 신호 서열을 포함할 수 있으며, 예를 들어, 내인성 DR6 신호 서열 및 선택적으로 내인성 DR6 막 관통 도메인과 작동가능하게 연결되도록 DR6 유전자좌에 위치할 수 있다. 이러한 경우, DR6 유전자좌에 대해 외인성으로 위치하거나, 내인성 DR6 유전자좌에 위치한 삽입 핵산에 의해 인코딩되는 임의의 단백질(들) (예를 들어, 리포터)의 목적지는 DR6의 목적지와 유사하다 (예를 들어, 내인성 막 관통 도메인에 의해 막에 고정됨). 일 실시 형태에서, 삽입 핵산은 내인성 막 관통 도메인을 대체할 수 있다. 이러한 경우와 다른 경우에, 삽입 핵산은, 이종성 막 관통 도메인을 인코딩하는 제2 핵산을 포함할 수 있다. 일 실시 형태에서, 삽입 핵산은 이종성 막 관통 도메인 유전자에 작동가능하게 연결된 리포터 유전자를 포함하며, 삽입 핵산은, 내인성 DR6 프로모터에 의해 상기 삽입 핵산의 발현이 유도되도록 내인성 DR6 신호 서열에 작동가능하게 연결된 DR6 유전자에 삽입되고, 상기 삽입 핵산에 의해 인코딩되는 단백질(들)은 신호 서열에 따라 막에 고정된다.
일 실시 형태에서, DR6 유전자좌 또는 그 일부분은 선택가능한 마커를 인코딩하는 추가의 핵산 서열을 포함하는 삽입 핵산으로 대체된다. 이러한 경우, 상기 추가의 핵산 서열은 선택가능한 마커의 발현을 유도하는 프로모터에 작동가능하게 연결된다.
다른 실시 형태에서, DR6 유전자좌 또는 그 일부분은 막 관통 유전자, 리포터 유전자 및 선택가능한 마커 유전자를 포함하는 삽입 핵산으로 대체된다. 이러한 경우, 상기 리포터 유전자 및/또는 선택가능한 마커 유전자는 프로모터에 작동가능하게 연결될 수 있거나, 그러하지 않을 수 있다.
상기 방법 및 조성에 사용될 수 있는 다양한 프로모터가 본 명세서의 다른 부분에 제공된다.
이러한 유전자 변형 (표적 DR6의 발현 및/또는 활성의 저하 또는 조절을 야기하는 것을 포함함)은 또한 생식세포계열을 통해 전달될 수 있다. 구체적인 실시 형태에서, 유전자 변형은 원하는 표적 유전자좌의 녹아웃을 야기한다. 예를 들어, 이러한 비-인간 동물은 본 명세서의 다른 부분에서 논의된 바와 같이 다양한 실험 시스템에서 사용된다.
예를 들어, DR6 녹아웃은 DR6 기능, 발생 중 DR6의 역할, 및 다양한 세포 경로 및 질환, 특히 운동 뉴런 기능 장애, 예를 들어 ALS에서의 DR6의 역할을 연구하기 위한 동물 모델을 제공한다.
비-인간 동물, 세포, 조직 또는 배아에서 DR6 유전자좌의 유전자 변형을 위한 방법 및 조성이 본 명세서에 제공된다.
DR6 유전자좌의 유전자 변형은 본 명세서의 다른 부분에 상세히 기재된 바와 같은 상기 유전자좌의 임의의 변형 (즉, 결실, 삽입, 대체 등)일 수 있다. 이러한 경우, 상기 유전자 변형은 DR6의 기능 상실을 야기한다. 일 실시 형태에서, 유전자 변형은 DR6의 분열 또는 녹아웃을 포함한다.
A. 리포터의 녹인 대립유전자의 설계 및 제작
비제한적인 예로서, 삽입 핵산이 내인성 신호 서열에 작동가능하게 연결될 수 있도록 제2 엑손에 결실 개시점을 설정할 수 있다. 도 1은 DR6을 코딩하는 서열의 전부 또는 대부분의 표적화된 결실 및 ROR1 (수용체 티로신 키나제-유사 고아 수용체 1 (receptor tyrosine kinase-like orphan receptor 1)) 유전자로부터의 막 관통 도메인 서열, β-갈락토시다제를 인코딩하는 이. 콜라이(E. coli) lacZ 유전자의 서열을 함유하는 카세트 및 G418-저항성 ES 세포 콜로니의 선택을 위한 네오마이신 포스포트랜스퍼라제를 발현하는 카세트 (neor)로의 대체의 예를 제시한다. 표현형 분석 전에 Cre-매개 절제를 가능하게 하는 loxP 재조합효소 인식 부위는 약물 선택 카세트를 플랭킹한다.
LTVEC 표적화 벡터를 ES 세포에 도입하고, 대립유전자 변형 검정 (modification-of-allele assay) (문헌[Frendewey, D., et al. (2010), Methods Enzymol 476, 295-307])에 의해 정확하게 표적화된 클론에 대해 스크리닝할 수 있다.
다양한 방법을 사용하여 결실 또는 삽입과 같은 표적화된 변형을 갖는 세포를 확인할 수 있다. 이러한 방법은 표적 유전자좌에 표적화된 변형을 갖는 하나의 세포를 확인하는 단계를 포함할 수 있다. 스크리닝을 수행하여 변형된 게놈 유전자좌를 갖는 이러한 세포를 확인할 수 있다.
스크리닝 단계는 모 염색체의 대립유전자 변형 (MOA)을 평가하는 정량적인 검정을 포함할 수 있다. 예를 들어, 실시간 PCR과 같은 정량적인 PCR (qPCR)을 통해 정량적인 검정을 수행할 수 있다. 실시간 PCR은 표적 유전자좌를 인식하는 제1 프라이머 세트 및 비표적화된 기준 유전자좌를 인식하는 제2 프라이머 세트를 이용할 수 있다. 프라이머 세트는 증폭된 서열을 인식하는 형광 프로브를 포함할 수 있다.
적합한 정량적인 검정의 다른 예는 형광 매개 원위치 혼성화(fluorescence-mediated in situ hybridization, FISH), 비교 게놈 혼성화, 등온 DNA 증폭, 고정화된 프로브(들)에 대한 정량적인 혼성화, Invader Probes®, MMP assays®, TaqMan® 분자 비콘(Molecular Beacon) 또는 Eclipse™ 프로브 기술을 포함한다 (예를 들어, 본 명세서에 전체적으로 참고로 포함된 미국 특허 출원 공개 제2005/0144655호 참조).
VelociMouse® 방법 (문헌[Poueymirou, WT, et al. (2007), Nat Biotechnol 25, 91-99])이, 표적화된 ES 세포를, 동형접합성으로의 증식 또는 lacZ 발현 프로파일링을 위해 준비된 완전한 ES 세포-유래 F0 세대 이형접합 마우스로 전환시키기 위해 8-세포 배아 단계 주입에 적용될 수 있다. ZEN-Ub1 카세트를 보유한 마우스는, 임의의 loxP에 플랭킹된 neor 카세트를 제거하기 위해, Cre 결실자 마우스 계통 (예를 들어, 국제 특허 공개 WO 2009/114400호 참조)으로 증식될 수 있다.
DR6 리포터 녹인 동물을 생산하는 방법의 추가 세부 사항은 본 명세서의 실시예 1에 제공되어 있다.
B. 리포터 발현 프로파일링
본 명세서의 다른 부분에 기재된 바와 같이, DR6 유전자좌의 유전자 변형은 DR6 유전자좌 또는 그 일부분의 삽입 핵산으로의 대체 또는 삽입/첨가를 포함할 수 있다. 일부의 경우, 삽입 핵산은 리포터 유전자를 포함한다. 일 실시 형태에서, 리포터 유전자는 내인성 DR6 프로모터와 작동가능하게 연결되어 DR6 유전자좌에 위치한다. 이러한 변형은 내인성 DR6 프로모터에 의해 유도되는 리포터 유전자의 발현을 가능하게 한다. 대안적으로, 리포터 유전자는 내인성 DR6 프로모터와 작동가능하게 연결되어 위치하지 않는다.
임의의 리포터 (또는 검출가능한 모이어티(moiety))가, 잘 알려진 방법 및 조성으로 사용될 수 있다. 리포터의 비제한적인 예는, 예를 들어, β-갈락토시다제 (lacZ 유전자에 의해 인코딩됨), 녹색 형광 단백질 (GFP), 증강 녹색 형광 단백질 (eGFP), 엠플럼(mPlum), 엠체리(mCherry), 티디토마토(tdTomato), 엠스트로베리(mStrawberry), 제이-레드(J-Red), 디에스레드(DsRed), 엠오렌지(mOrange), mKO, 엠시트린(mCitrine), 베누(Venu), 와이펫(YPet), 증강 황색 형광 단백질 (EYFP), 에메랄드(Emerald), 사이펫(CyPet), 시안 형광 단백질 (CFP), 세룰리안(Cerulean), T-사파이어(T-Sapphire), 루시퍼라제, 알칼리 포스파타제, 또는 이들의 조합을 포함한다.
본 명세서에 기재된 방법 및 조성은 리포터 유전자의 부재하에 또는 임의의 리포터 유전자의 존재 하에 수행될 수 있다. 하기 설명은 β-갈락토시다제를 인코딩하는 lacZ 리포터 유전자를 이용하는 비제한적인 예이다.
C. 표현형
내인성 DR6 유전자좌의 유전자 변형은 본 명세서에 제공된 비-인간 동물에서 다양한 표현형을 야기할 수 있다. 일 실시 형태에서, 내인성 DR6 유전자좌의 유전자 변형은 출생 시에 지극히 정상이나, 나이가 든 때에, 예를 들어, 1주령 후, 2주령 후, 3주령 후, 4주령 후, 5주령 후, 6주령 후, 7주령 후, 8주령 후 등에 ALS-유사 증상이 발생하는 비-인간 동물을 야기한다. 다른 실시 형태에서, 내인성 DR6 유전자좌의 유전자 변형은 하나 이상의 세포 유형, 예를 들어 뉴런 및/또는 신경교세포 및/또는 그 일부분, 예를 들어, 마이엘린의 비정상적인 기능을 야기한다. 뉴런은 감각 뉴런, 운동 뉴런, 및 중간 뉴런으로 보통 지칭되는 기타 모든 뉴런 유형을 포함한다. 신경교세포는 성상교세포, 희소돌기아교세포 등을 포함한다.
용어 "ALS 유사 증상" 등은 일반적으로 "ALS와 관련된 증상" 또는 "상위 및/또는 하위 운동 뉴런 기능 장애로 인한 증상"을 의미한다. ALS 유사 증상은 뉴런, 예를 들어 운동 뉴런, 감각 뉴런 및/또는 중간 뉴런의 손상을 포함할 수 있다. 예를 들어, 상위 운동 뉴런과 관련된 ALS 유사 증상은 경직 (예를 들어, 경직성 마비, 강직), 증가되고/되거나 비정상적인 반사 (예를 들어, 바빈스키 징후), 진전 및 이들의 조합을 야기할 수 있다. 하위 운동 뉴런의 손상과 관련된 ALS 유사 증상은 근육 약화와 소모, 근섬유다발수축 및 이들의 조합, 및/또는 삼키는 동작의 불능을 야기하는 연수 손상 및 혀의 근섬유다발수축을 야기할 수 있다. ALS 유사 증상은 또한 하기 표현형 중 하나 이상을 포함할 수 있다: a) 척추후만증; b) 비정상적인 뒷다리 움켜쥐기, 끌기 또는 발가락 구부러짐; c) 운동 협응과 운동 학습 능력의 결손, 로타로드, 캣워크 및/또는 오픈 필드 테스트(들)에서의 결손; d) 척수에서의 운동 뉴런 상실; e) 척수에서의 성상세포증; f) 대조군 설치류와 비교한 중량 감소; g) 폴리-유비퀴틴화 단백질의 축적; (h) ALS-TDI 신경학적 점수화 시스템을 사용한 신경학적 점수의 증가 및/또는 (i) 통증 자극에 대한 반응 지체 시간의 증가.
ALS-TDI 신경학적 점수화 시스템
Figure pat00002
II. 비-인간 동물에서 내인성 DR6 유전자좌를 변형시키는 방법
비-인간 동물, 세포, 조직 또는 배아에서 내인성 DR6 유전자좌를 유전자 변형시키는 방법이 본 명세서에 제공된다. 일 실시 형태에서, 만능성 세포에서 DR6 유전자좌를 변형시키는 방법이 제공된다. 이러한 방법은 (a) DR6 유전자좌와의 상동성 재조합을 거칠 수 있는 5' 및 3' 상동성 아암에 의해 플랭킹된 삽입 핵산을 포함하는 표적화 작제물을 만능성 세포로 도입시키는 단계; 및 (b) DR6 유전자좌에서 표적화된 유전자 변형을 포함하는 변형 만능성 세포를 확인하는 단계를 포함한다. 이러한 방법에서, 유전자 변형은 DR6의 기능 상실을 야기한다. 일 실시 형태에서, 만능성 세포는 설치류 배아 줄기이다. 다른 실시 형태에서, 만능성 세포는 인간 iPS 세포이다.
A. 표적화 벡터 및 삽입 핵산
본 명세서에 제공된 유전자 변형 비-인간 동물, 세포, 조직 또는 배아를 제조하는 방법에 사용되는 표적화 벡터 또는 표적화 작제물이 추가로 제공된다.
일 실시 형태에서, DR6 유전자좌와의 상동성 재조합을 거칠 수 있는 5' 및 3' 상동성 아암에 의해 플랭킹된 삽입 핵산을 포함하는 표적화 벡터가 제공된다.
표적화 벡터 및 상기 표적화 벡터의 성분 (즉, 삽입 핵산, 관심 폴리뉴클레오티드, 발현 카세트 등)의 예가 하기에 본 명세서에 상세하게 기재되어 있다.
i. 삽입 핵산
용어 "삽입 핵산" 또는 "삽입 폴리뉴클레오티드"는 표적 유전자좌에 통합하기를 원하는 DNA의 분절을 포함한다. 일 실시 형태에서, 삽입 핵산은 하나 이상의 관심 폴리뉴클레오티드를 포함한다. 다른 실시 형태에서, 삽입 핵산은 하나 이상의 발현 카세트를 포함할 수 있다. 주어진 발현 카세트는 관심 폴리뉴클레오티드, 선택 마커 및/또는 리포터 유전자를 인코딩하는 폴리뉴클레오티드와 함께 발현에 영향을 미치는 다양한 조절 성분을 포함할 수 있다.
임의의 관심 폴리뉴클레오티드가 다양한 삽입 폴리뉴클레오티드에 삽입되어 표적 게놈 유전자좌에 통합될 수 있다. 본 명세서에 개시된 방법은 표적화된 DR6 게놈 유전자좌에 통합될 적어도 1개, 2개, 3개, 4개, 5개, 6개 또는 그 이상의 관심 폴리뉴클레오티드를 제공한다.
일 실시 형태에서, 삽입 핵산에 함유된 관심 폴리뉴클레오티드는 리포터를 인코딩한다. 다른 실시 형태에서, 관심 폴리뉴클레오티드는 선택가능한 마커를 인코딩한다.
일 실시 형태에서, 관심 폴리뉴클레오티드는 부위-특이적 재조합 부위에 의해 플랭킹될 수 있다. 구체적인 실시 형태에서, 부위-특이적 재조합 부위는 리포터를 인코딩하는 분절 및/또는 선택가능한 마커를 인코딩하는 분절을 플랭킹한다.
삽입 핵산 내에 포함될 수 있는 선택 마커 및 리포터 유전자를 포함하는 관심 폴리뉴클레오티드의 비제한적인 예는 본 명세서의 다른 부분에서 상세히 논의된다.
표적 DR6 유전자좌에 통합될 때 삽입 폴리뉴클레오티드 내의 관심 폴리뉴클레오티드는 세포 내로 하나 이상의 유전자 변형을 도입할 수 있다. 유전자 변형은 내인성 핵산 서열의 결실 및/또는 표적 게놈 유전자좌 내로의 외인성 또는 이종성 또는 이종상동성 폴리뉴클레오티드의 첨가를 포함할 수 있다. 일 실시 형태에서, 유전자 변형은 표적 게놈 유전자좌에서 내인성 핵산 서열의 관심 외인성 폴리뉴클레오티드로의 대체를 포함한다. 따라서, 본 명세서에 제공되는 방법은 표적 DR6 유전자좌에서의 녹아웃, 결실, 삽입, 대체 ("녹인"), 점 돌연변이, 도메인 교체, 엑손 교체, 인트론 교체, 조절 서열 교체, 유전자 교체 또는 이들의 조합을 포함하는 유전자 변형의 생성을 가능하게 한다. 이러한 변형은 제1, 제2, 제3, 제4, 제5, 제6, 제7 또는 임의의 후속 삽입 폴리뉴클레오티드를 표적 게놈 유전자좌로 통합 시에 발생할 수 있다.
삽입 폴리뉴클레오티드 내에 있고/있거나 표적 게놈 유전자좌에 통합된 관심 폴리뉴클레오티드는 그가 도입되는 세포에 상동성이거나 고유한 서열을 포함할 수 있거나; 관심 폴리뉴클레오티드는 그가 도입되는 세포에 이종성일 수 있거나; 관심 폴리뉴클레오티드는 그가 도입되는 세포에 외인성일 수 있거나; 관심 폴리뉴클레오티드는 그가 도입되는 세포에 이종상동성일 수 있거나; 또는 관심 폴리뉴클레오티드는 그가 도입되는 세포와 상이한 종으로부터 유래된 것일 수 있다. 서열과 관련하여, 용어 "상동성"은 세포에 고유한 서열이다. 서열과 관련하여, 용어 "이종성"은 외래종으로부터 유래된 서열이거나, 또는 동일 종으로부터 유래된 경우에는, 인간의 의도적인 개입에 의해 조성 및/또는 게놈 유전자좌에서 그의 고유한 형태로부터 실질적으로 변형된 서열이다. 서열과 관련하여, 용어 "외인성"은 외래종으로부터 유래된 서열이다. 용어 "이종상동성"은 다른 종 (즉, 종 변이체)에서의 공지된 참조 서열과 기능적으로 동등한 하나의 종으로부터 유래된 폴리뉴클레오티드이다. 관심 폴리뉴클레오티드는 원핵생물, 진핵생물, 비-인간, 설치류, 햄스터, 마우스, 래트, 인간, 원숭이, 조류, 농업용 포유류 또는 비농업용 포유류를 포함하지만 이에 한정되지 않는 임의의 관심 유기체로부터 유래될 수 있다. 관심 폴리뉴클레오티드는 코딩 영역, 비코딩 영역, 조절 영역 또는 게놈 DNA를 추가로 포함할 수 있다. 따라서, 제1, 제2, 제3, 제4, 제5, 제6, 제7 및/또는 임의의 후속 삽입 폴리뉴클레오티드는 이러한 서열을 포함할 수 있다.
일 실시 형태에서, 관심 폴리뉴클레오티드는 상기에 기재된 바와 같이, 약 500개의 뉴클레오티드 내지 약 200 kb의 범위일 수 있다. 관심 폴리뉴클레오티드는 약 500개의 뉴클레오티드 내지 약 5 kb, 약 5 kb 내지 약 200 kb, 약 5 kb 내지 약 10 kb, 약 10 kb 내지 약 20 kb, 약 20 kb 내지 약 30 kb, 약 30 kb 내지 약 40 kb, 약 40 kb 내지 약 50 kb, 약 60 kb 내지 약 70 kb, 약 80 kb 내지 약 90 kb, 약 90 kb 내지 약 100 kb, 약 100 kb 내지 약 110 kb, 약 120 kb 내지 약 130 kb, 약 130 kb 내지 약 140 kb, 약 140 kb 내지 약 150 kb, 약 150 kb 내지 약 160 kb, 약 160 kb 내지 약 170 kb, 약 170 kb 내지 약 180 kb, 약 180 kb 내지 약 190 kb 또는 약 190 kb 내지 약 200 kb일 수 있다.
폴리뉴클레오티드 내에 있고/있거나 표적 게놈 유전자좌에 삽입된 관심 폴리뉴클레오티드는 폴리펩티드를 인코딩할 수 있거나, RNA를 인코딩할 수 있거나, 이것은, 예를 들어 조절 서열, 프로모터 서열, 인핸서 서열, 전사 억제인자 결합 서열, Kozak 공통 분절, 개시 코돈을 포함하는 임의의 관심 조절 영역 또는 비코딩 영역, 또는 비단백질 코딩(non-protein-coding) 서열의 결실을 포함할 수 있지만, 단백질 코딩 서열의 결실은 포함하지 않는다. 게다가, 삽입 폴리뉴클레오티드 내에 있고/있거나 표적 게놈 유전자좌에 삽입된 관심 폴리뉴클레오티드는 신경계, 골격계, 소화계, 순환계, 근육계, 호흡계, 심혈관계, 림프계, 내분비계, 비뇨계, 생식계 또는 이들의 조합에서 발현되는 단백질을 인코딩할 수 있다.
일 실시 형태에서, 삽입 핵산은 내인성 유전자의 적어도 하나의 엑손의 녹인 대립유전자를 포함한다. 일 실시 형태에서, 삽입 핵산은 전체 내인성 유전자의 녹인 대립유전자 (즉, "유전자-교체 녹인")를 포함한다.
일 실시 형태에서, 삽입 핵산은, 예를 들어 프로모터, 인핸서 또는 전사 억제인자 결합 요소를 포함하는 조절 요소를 포함한다.
추가의 실시 형태에서, 삽입 핵산은 조건 대립유전자를 포함한다. 일 실시 형태에서, 조건 대립유전자는, 본 명세서에 전체적으로 참고로 포함된 미국 특허 출원 공개 제2011/0104799호에 기재된 바와 같은, 다기능 대립유전자이다. 구체적인 실시 형태에서, 조건 대립유전자는 하기를 포함한다: (a) 표적 유전자의 전사와 관련된 센스 배향의 작동 서열, 및 센스 또는 안티센스 배향의 약물 선택 카세트; (b) 안티센스 배향의 관심 뉴클레오티드 서열 (NSI) 및 역전 모듈에 의한 조건 대립유전자 (COIN, 엑손-분할 인트론 및 가역적 유전자트랩형 모듈 사용; 예를 들어 전체적으로 참고로 포함된 미국 특허 출원 공개 제2011/0104799호 참조); 및 (c) 제1 재조합효소에 노출 시에 재조합되어, (i) 작동 서열 및 DSC가 결여되어 있고, (ii) 센스 배향의 NSI 및 안티센스 배향의 COIN을 함유하는 조건 대립유전자를 형성하는 재조합가능한 단위.
일 실시 형태에서, 삽입 핵산은 코딩 서열의 유전자 변형을 포함한다. 일 실시 형태에서, 유전자 변형은 코딩 서열의 결실 돌연변이를 포함한다. 일 실시 형태에서, 유전자 변형은 2개의 내인성 코딩 서열의 융합을 포함한다.
일 실시 형태에서, 유전자 변형은 비단백질 코딩 서열의 결실을 포함하나, 단백질 코딩 서열의 결실은 포함하지 않는다. 일 실시 형태에서, 비단백질 코딩 서열의 결실은 DR6 유전자좌 또는 그 일부분의 결실을 포함한다. 일 실시 형태에서, 비단백질 코딩 서열의 결실은 조절 요소의 결실을 포함한다. 일 실시 형태에서, 유전자 변형은 프로모터의 결실을 포함한다. 일 실시 형태에서, 유전자 변형은 프로모터 또는 조절 요소의 첨가를 포함한다. 일 실시 형태에서, 유전자 변형은 프로모터 또는 조절 요소의 대체를 포함한다.
일 실시 형태에서, 표적화 벡터의 핵산 서열은, 게놈에 통합되면, 포유류, 비-인간 동물 또는 비-인간 포유류 DR6 유전자좌의 영역의 유전자 변형을 생성하는 폴리뉴클레오티드를 포함할 수 있으며, DR6 유전자좌의 유전자 변형은 DR6의 기능 상실을 야기한다. 일 실시 형태에서, DR6 녹아웃 ("널 대립유전자")이 생성된다. 다른 실시 형태에서, DR6 유전자좌의 분열이 생성된다.
추가의 실시 형태에서, 삽입 핵산은 포유류, 비-인간 동물 또는 비-인간 포유류 DR6 유전자좌의 일부분의 삽입 핵산 서열로의 대체를 야기한다. 일 실시 형태에서, 삽입 핵산 서열은 리포터 핵산 서열이다.
주어진 삽입 폴리뉴클레오티드 및 대체되는 포유류, 비-인간 또는 비-인간 포유류 유전자좌의 상응하는 영역은 비-코딩 영역, 코딩 영역, 인트론, 엑손, 비번역 영역, 조절 영역, 프로모터, 또는 인핸서 또는 이들의 임의의 조합일 수 있다. 또한, 주어진 삽입 폴리뉴클레오티드 및/또는 결실되는 포유류, 비-인간 또는 비-인간 포유류 유전자좌의 영역은, 예를 들어, 10 내지 100개의 뉴클레오티드 길이, 100 내지 500개의 뉴클레오티드 길이, 500개 내지 1 kb의 뉴클레오티드 길이, 1 kb 내지 1.5 kb의 뉴클레오티드 길이, 1.5 kb 내지 2 kb의 뉴클레오티드 길이, 2 kb 내지 2.5 kb의 뉴클레오티드 길이, 2.5 kb 내지 3 kb의 뉴클레오티드 길이, 3 kb 내지 5 kb의 뉴클레오티드 길이, 5 kb 내지 8 kb의 뉴클레오티드 길이, 8 kb 내지 10 kb의 뉴클레오티드 길이 이상을 포함하는 임의의 원하는 길이일 수 있다. 다른 예에서, 삽입 또는 대체되는 크기는 약 5 kb 내지 약 10 kb, 약 10 kb 내지 약 20 kb, 약 20 kb 내지 약 40 kb, 약 40 kb 내지 약 60 kb이다. 다른 실시 형태에서, 주어진 삽입 폴리뉴클레오티드 및/또는 결실되는 포유류, 인간 세포 또는 비-인간 포유류 유전자좌의 영역은 적어도 100, 200, 300, 400, 500, 600, 700, 800, 또는 900개의 뉴클레오티드 또는 적어도 1 kb, 2 kb, 3 kb, 4 kb, 5 kb, 6 kb, 7 kb, 8 kb, 9 kb, 10 kb, 11 kb, 12 kb, 13 kb, 14 kb, 15k b, 16 kb, 17 kb, 18 kb, 19 kb, 20 kb, 25 kb, 30 kb, 35 kb, 40 kb, 45 kb, 50 kb 이상이다.
일 실시 형태에서, 삽입 핵산은, 내인성 DR6 프로모터에 작동가능하게 연결되도록 관심 DR6 유전자좌에 삽입된다. 이러한 경우, DR6 프로모터는 삽입 핵산 서열의 발현을 유도한다. 일 실시 형태에서, 삽입 핵산 서열은 리포터 핵산 서열이다.
일부의 경우, 삽입 핵산은 프로모터를 포함한다. 일 실시 형태에서, 삽입 핵산은, 관심 폴리뉴클레오티드의 발현을 유도하는 프로모터에 작동가능하게 연결된 관심 폴리뉴클레오티드를 포함한다. 일 실시 형태에서, 관심 폴리뉴클레오티드는 리포터 핵산 서열을 포함한다. 다른 실시 형태에서, 관심 폴리뉴클레오티드는 선택 마커 핵산 서열을 포함한다.
일 실시 형태에서, 프로모터는 항시적 활성 프로모터이다.
일 실시 형태에서, 프로모터는 유도성 프로모터이다. 일 실시 형태에서, 유도성 프로모터는 화학적 조절 프로모터이다. 일 실시 형태에서, 화학적 조절 프로모터는 알코올-조절 프로모터이다. 일 실시 형태에서, 알코올-조절 프로모터는 알코올 탈수소효소 (alcA) 유전자 프로모터이다. 일 실시 형태에서, 화학적 조절 프로모터는 테트라사이클린-조절 프로모터이다. 일 실시 형태에서, 테트라사이클린-조절 프로모터는 테트라사이클린-반응성 프로모터이다. 일 실시 형태에서, 테트라사이클린-조절 프로모터는 테트라사이클린 작동자 서열 (tetO)이다. 일 실시 형태에서, 테트라사이클린-조절 프로모터는 tet-On 프로모터이다. 일 실시 형태에서, 테트라사이클린-조절 프로모터는 tet-Off 프로모터이다. 일 실시 형태에서, 화학적 조절 프로모터는 스테로이드 조절 프로모터이다. 일 실시 형태에서, 스테로이드 조절 프로모터는 래트의 글루코코르티코이드 수용체의 프로모터이다. 일 실시 형태에서, 스테로이드 조절 프로모터는 에스트로겐 수용체의 프로모터이다. 일 실시 형태에서, 스테로이드 조절 프로모터는 엑디손 수용체의 프로모터이다. 일 실시 형태에서, 화학적 조절 프로모터는 금속 조절 프로모터이다. 일 실시 형태에서, 금속 조절 프로모터는 금속단백질 프로모터이다. 일 실시 형태에서, 유도성 프로모터는 물리적 조절 프로모터이다. 일 실시 형태에서, 물리적 조절 프로모터는 온도 조절 프로모터이다. 일 실시 형태에서, 온도 조절 프로모터는 열 충격 프로모터이다. 일 실시 형태에서, 물리적 조절 프로모터는 광 조절 프로모터이다. 일 실시 형태에서, 광 조절 프로모터는 광 유도성 프로모터이다. 일 실시 형태에서, 광 조절 프로모터는 광 억제성 프로모터이다.
일 실시 형태에서, 프로모터는 조직 특이적 프로모터이다. 일 실시 형태에서, 프로모터는 뉴런 특이적 프로모터이다. 일 실시 형태에서, 프로모터는 신경교 특이적 프로모터이다. 일 실시 형태에서, 프로모터는 근육 세포 특이적 프로모터이다. 일 실시 형태에서, 프로모터는 심장 세포 특이적 프로모터이다. 일 실시 형태에서, 프로모터는 신장 세포 특이적 프로모터이다. 일 실시 형태에서, 프로모터는 골 세포 특이적 프로모터이다. 일 실시 형태에서, 프로모터는 내피 세포 특이적 프로모터이다. 일 실시 형태에서, 프로모터는 면역 세포 특이적 프로모터이다. 일 실시 형태에서, 면역 세포 프로모터는 B 세포 프로모터이다. 일 실시 형태에서, 면역 세포 프로모터는 T 세포 프로모터이다.
일 실시 형태에서, 프로모터는 발생적 조절 프로모터이다. 일 실시 형태에서, 발생적 조절 프로모터는 배아 발생 단계에서만 활성이다. 일 실시 형태에서, 발생적 조절 프로모터는 성체 세포에서만 활성이다.
구체적인 실시 형태에서, 프로모터는 세포 유형에 기초하여 선택될 수 있다. 따라서, 다양한 프로모터가 진핵 세포, 포유류 세포, 비인간 포유류 세포, 만능성 세포, 비인간 만능성 세포, 인간 만능성 세포, 인간 ES 세포, 인간 성체 줄기 세포, 발생적으로 제한된 인간 전구 세포, 인간 iPS 세포, 인간 세포, 설치류 세포, 래트 세포, 마우스 세포, 햄스터 세포, 섬유아세포 또는 CHO 세포에서 사용된다.
일부 실시 형태에서, 삽입 핵산은 부위-특이적 재조합 표적 서열이 플랭킹된 핵산을 포함한다. 전체 삽입 핵산이 이러한 부위-특이적 재조합 표적 서열에 의해 플랭킹될 수 있지만, 삽입 핵산 내의 임의의 영역 또는 개별 관심 폴리뉴클레오티드가 또한 이러한 부위에 의해 플랭킹될 수 있는 것으로 인식된다. 부위-특이적 재조합효소는, 재조합효소 폴리펩티드를 세포 내로 도입하거나 부위-특이적 재조합효소를 인코딩하는 폴리뉴클레오티드를 숙주 세포 내로 도입하는 것을 포함하는 임의의 수단에 의해 세포 내로 도입될 수 있다. 부위-특이적 재조합효소를 인코딩하는 폴리뉴클레오티드는 삽입 핵산 내에 또는 개별 폴리뉴클레오티드 내에 위치할 수 있다. 부위-특이적 재조합효소는, 예를 들어, 유도성 프로모터, 세포에 대해 내인성인 프로모터, 세포에 대해 이종성인 프로모터, 세포 특이적 프로모터, 조직 특이적 프로모터, 또는 발생 단계 특이적 프로모터를 포함하는, 세포에서 활성인 프로모터에 작동가능하게 연결될 수 있다. 삽입 핵산 또는 삽입 핵산 내의 임의의 관심 폴리뉴클레오티드를 플랭킹할 수 있는 부위-특이적 재조합 표적 서열은 loxP, lox511, lox2272, lox66, lox71, loxM2, lox5171, FRT, FRT11, FRT71, attp, att, FRT, rox, 또는 이들의 조합을 포함할 수 있지만 이에 한정되지 않는다.
일부 실시 형태에서, 부위-특이적 재조합 부위는 삽입 핵산 내에 함유된 선택 마커를 인코딩하는 폴리뉴클레오티드 및/또는 리포터 유전자를 플랭킹한다. 그러한 경우에, 표적화된 유전자좌에서의 삽입 핵산의 통합 후에, 부위-특이적 재조합 부위들 사이의 서열이 제거될 수 있다.
일 실시 형태에서, 삽입 핵산은 선택 마커를 인코딩하는 폴리뉴클레오티드를 포함한다. 선택 마커는 선택 카세트에 함유될 수 있다. 이러한 선택 마커는 네오마이신 포스포트랜스퍼라제(neor), 하이그로마이신 B 포스포트랜스퍼라제(hygr), 퓨로마이신-N-아세틸트랜스퍼라제(puror), 블라스티사이딘 S 데아미나제(bsrr), 잔틴/구아닌 포스포리보실 트랜스퍼라제(gpt), 또는 단순 포진 바이러스 티미딘 키나제(HSV-k), 또는 이들의 조합을 포함하지만 이에 한정되지 않는다. 일 실시 형태에서, 선택 마커를 인코딩하는 폴리뉴클레오티드는 세포에서 활성인 프로모터에 작동가능하게 연결된다. 일 실시 형태에서, 선택 마커를 인코딩하는 폴리뉴클레오티드에는 부위-특이적 재조합 표적 서열이 플랭킹된다.
삽입 핵산은 프로모터에 작동가능하게 연결된 리포터 유전자를 추가로 포함할 수 있으며, 상기 리포터 유전자는 β-갈락토시다제 (lacZ 유전자에 의해 인코딩됨), GFP, 엠플럼, 엠체리, 티디토마토, 엠스트로베리, 제이-레드, 디에스레드, 엠오렌지, mKO, 엠시트린, 베누, 와이펫, 증강 황색 형광 단백질 (EYFP), 에메랄드, 증강 녹색 형광 단백질 (EGFP), 사이펫, 시안 형광 단백질 (CFP), 세룰리안, T-사파이어, 루시퍼라제, 알칼리 포스파타제, 및/또는 이들의 조합으로 이루어지거나 이를 포함하는 군으로부터 선택되는 리포터 단백질을 인코딩한다. 이러한 리포터 유전자는 세포에서 활성인 프로모터에 작동가능하게 연결될 수 있다. 이러한 프로모터는 유도성 프로모터, 리포터 유전자 또는 세포에 대해 내인성인 프로모터, 리포터 유전자 또는 세포에 대해 이종성인 프로모터, 세포 특이적 프로모터, 조직 특이적 프로모터 또는 발생 단계 특이적 프로모터일 수 있다.
ii. 발현 카세트
DR6 유전자좌를 표적화하기 위해 본 명세서에 제공된 표적화된 게놈 통합 시스템에 사용되는 다양한 성분 (즉, 뉴클레아제 제제, 인식 부위, 삽입 핵산, 관심 폴리뉴클레오티드, 리포터 서열, 표적화 벡터, 선택 마커 및 다른 성분 중 임의의 하나 또는 임의의 조합)을 포함하는 폴리뉴클레오티드 또는 핵산 분자가 본 명세서에 제공된다.
용어 "폴리뉴클레오티드", "폴리뉴클레오티드 서열", "핵산 서열" 및 "핵산 단편"은 본 명세서에서 상호교환적으로 사용된다. 이들 용어는 뉴클레오티드 서열 등을 포함한다. 폴리뉴클레오티드는, 선택적으로 합성, 비-천연 또는 변이된 뉴클레오티드 염기를 함유하는 단일- 또는 이중-가닥인 RNA 또는 DNA의 중합체일 수 있다. DNA의 중합체 형태의 폴리뉴클레오티드는 cDNA, 게놈 DNA, 합성 DNA, 또는 이들의 혼합물의 하나 이상의 분절로 구성될 수 있다. 폴리뉴클레오티드는 천연 분자 및 합성 유사체 둘 모두를 포함하는 데옥시리보뉴클레오티드 및 리보뉴클레오티드, 및 이들의 임의의 조합을 포함할 수 있다. 본 명세서에 제공된 폴리뉴클레오티드는 또한 단일 가닥 형태, 이중 가닥 형태, 헤어핀, 스템-앤드-루프 구조(stem-and-loop structure) 등을 포함하지만 이에 한정되지 않는 모든 형태의 서열을 포함한다.
DR6 유전자좌를 표적화하기 위한 표적화된 게놈 통합 시스템의 다양한 성분을 포함하는 재조합 폴리뉴클레오티드가 추가로 제공된다. 용어 "재조합 폴리뉴클레오티드" 및 "재조합 DNA 작제물"은 본 명세서에서 상호 교환적으로 사용된다. 재조합 작제물은 핵산 서열의 인공 또는 이종성 조합, 예를 들어 자연 상태에서는 함께 발견되지 않는 조절 서열 및 코딩 서열을 포함한다. 다른 실시 형태에서, 재조합 작제물은 상이한 공급원으로부터 유래된 조절 서열 및 코딩 서열, 또는 동일한 공급원으로부터 유래되지만 자연 상태에서 발견되는 것과 상이한 방식으로 배열된 조절 서열 및 코딩 서열을 포함할 수 있다. 이러한 작제물은 그 자체로 사용될 수 있거나 벡터와 함께 사용될 수 있다. 벡터가 사용되는 경우, 벡터의 선택은 당업자에게 잘 알려진 바와 같이 숙주 세포를 형질전환시키는 데 사용되는 방법에 의존한다. 예를 들어, 플라스미드 벡터가 사용될 수 있다. 본 명세서에 제공된 단리된 핵산 단편 중 임의의 것을 포함하는 숙주 세포를 성공적으로 형질전환, 선택 및 증식시키는 데 필요한 유전적 요소가 또한 제공된다. 스크리닝은 그 중에서도 DNA의 서던 분석, mRNA 발현의 노던(Northern) 분석, 단백질 발현의 면역블롯팅 분석, 또는 표현형 분석에 의해 달성될 수 있다.
구체적인 실시 형태에서, 본 명세서에 기재된 DR6 유전자좌를 표적화하기 위한 표적화된 게놈 통합 시스템의 하나 이상의 성분은 원핵 세포, 진핵 세포, 박테리아, 효모 세포 또는 포유류 세포 또는 다른 관심 유기체 또는 세포 유형에서의 발현을 위한 발현 카세트에 제공될 수 있다. 카세트는 본 명세서에 제공된 폴리뉴클레오티드에 작동가능하게 연결된 5' 및 3' 조절 서열을 포함할 수 있다. "작동가능하게 연결된"은, 작동가능하게 연결된 성분이 그의 의도된 방식으로 기능하는 관계를 포함한다. 예를 들어, 관심 폴리뉴클레오티드와 조절 서열 (즉, 프로모터) 사이의 작동가능한 연결은 관심 폴리뉴클레오티드의 발현을 가능하게 하는 기능적 연결이다. 작동가능하게 연결된 요소는 인접하거나 인접하지 않을 수 있다. 2개의 단백질 코딩 영역의 결합을 지칭하는 데 사용될 때, '작동가능하게 연결된'은 코딩 영역이 동일한 리딩 프레임(reading frame)에 존재함을 의미한다. 다른 경우에, 단백질을 인코딩하는 핵산 서열이 적당한 전사 조절을 보유하도록 조절 서열 (예를 들어, 프로모터, 인핸서, 사일렌서 서열 등)에 작동가능하게 연결될 수 있다. 카세트는 유기체 내로 공동 도입되도록 적어도 하나의 추가의 관심 폴리뉴클레오티드를 추가로 함유할 수 있다. 대안적으로, 추가의 관심 폴리뉴클레오티드가 다수의 발현 카세트 상에 제공될 수 있다. 이러한 발현 카세트에는 조절 영역의 전사 조절 하에 있도록 재조합 폴리뉴클레오티드의 삽입을 위한 복수의 제한 부위 및/또는 재조합 부위가 제공된다. 발현 카세트는 추가적으로 선택 마커 유전자를 함유할 수 있다.
발현 카세트는, 5'-3'의 전사 방향으로, 관심 포유류 세포 또는 숙주 세포에서 기능성인 전사 및 번역 개시 영역 (즉, 프로모터), 본 명세서에 제공된 재조합 폴리뉴클레오티드, 및 전사 및 번역 종결 영역 (즉, 종결 영역)을 포함할 수 있다. 본 명세서에 제공된 조절 영역 (즉, 프로모터, 전사 조절 영역, Kozak 서열, 및 번역 종결 영역) 및/또는 폴리뉴클레오티드는 숙주 세포에 대해 또는 서로에 대해 고유/유사할 수 있다. 대안적으로, 본 명세서에 제공된 조절 영역 및/또는 폴리뉴클레오티드는 숙주 세포에 대해 또는 서로에 대해 이종성일 수 있다. 예를 들어, 이종 폴리뉴클레오티드에 작동가능하게 연결된 프로모터는 폴리뉴클레오티드가 유래된 종과 상이한 종으로부터 유래되거나, 동일한/유사한 종으로부터 유래된 경우, 하나 또는 둘 모두가 실질적으로 그들의 원래 형태 및/또는 게놈 유전자좌로부터 변형되거나, 프로모터는 작동가능하게 연결된 폴리뉴클레오티드의 고유 프로모터가 아니다. 대안적으로, 본 명세서에 제공된 조절 영역 및/또는 재조합 폴리뉴클레오티드는 전체적으로 합성일 수 있다.
종결 영역은 전사 개시 영역에 대해 고유할 수 있거나, 작동가능하게 연결된 재조합 폴리뉴클레오티드에 대해 고유할 수 있거나, 숙주 세포에 대해 고유할 수 있거나, 프로모터, 재조합 폴리뉴클레오티드, 숙주 세포, 또는 이들의 임의의 조합에 대해 다른 공급원 (즉, 외래 또는 이종성)으로부터 유래될 수 있다.
발현 카세트를 제조함에 있어서, 다양한 DNA 단편을 조작하여 적당한 배향으로 DNA 서열을 제공할 수 있다. 이러한 목적을 위해, 어댑터(adapter) 또는 링커(linker)를 사용하여 DNA 단편들을 결합할 수 있거나 다른 조작을 포함시켜 편리한 제한 부위, 피상적 DNA의 제거, 제한 부위의 제거 등을 제공할 수 있다. 이러한 목적을 위해, 시험관내 돌연변이 유발, 프라이머 수복, 제한, 어닐링, 재치환, 예를 들어 전이(transition) 및 역위(transversion)가 포함될 수 있다.
다수의 프로모터가 본 명세서에 제공된 발현 카세트에 사용될 수 있다. 프로모터는 원하는 결과에 기반하여 선택될 수 있다. 관심 폴리뉴클레오티드의 발현의 시기, 위치 및/또는 수준을 조절하기 위해 발현 카세트에서 다양한 프로모터를 사용하여 다양한 적용이 향상될 수 있음이 인식된다. 이러한 발현 작제물은 또한, 원하는 경우, 프로모터 조절 영역 (예를 들어, 유도성, 항시적, 환경적으로 또는 발생적으로 조절되는, 또는 세포- 또는 조직-특이적/선택적 발현을 부여하는 것), 전사 개시 부위, Kozak 공통 서열, 리보솜 결합 부위, RNA 프로세싱 신호, 전사 종결 부위 및/또는 폴리아데닐화 신호를 함유할 수 있다.
본 명세서에 제공된 폴리뉴클레오티드를 함유하는 발현 카세트는 또한 형질전환된 세포의 선택을 위한 선택 마커 유전자를 포함할 수 있다. 선택가능한 마커 유전자는 형질전환된 세포 또는 조직의 선택을 위해 이용된다.
적절한 경우, 본 방법 및 조성에 사용된 서열 (즉, 관심 폴리뉴클레오티드, 뉴클레아제 제제 등)은 세포에서의 발현의 증가를 위해 최적화될 수 있다. 즉, 유전자는 개선된 발현을 위해, 예를 들어 포유류 선호 코돈, 인간 선호 코돈, 설치류 선호 코돈, 마우스 선호 코돈, 래트 선호 코돈, 햄스터 선호 코돈 등을 포함하는, 주어진 관심 세포에서 선호되는 코돈을 사용하여 합성될 수 있다.
본 명세서에 제공되는 다양한 방법 및 조성은 선택 마커를 사용할 수 있다. 다양한 선택 마커가 본 명세서에 개시된 방법 및 조성에 사용될 수 있다. 그러한 선택 마커는, 예를 들어, 항생제, 예컨대 G418, 하이그로마이신, 블라스토사이딘, 네오마이신, 또는 퓨로마이신에 대한 저항성을 부여할 수 있다. 이러한 선택 마커는 네오마이신 포스포트랜스퍼라제 (neor), 하이그로마이신 B 포스포트랜스퍼라제 (hygr), 퓨로마이신-N-아세틸트랜스퍼라제 (puror) 및 블라스티사이딘 S 데아미나제 (bsrr)를 포함한다. 또 다른 실시 형태에서, 선택 마커는 유도성 프로모터에 작동가능하게 연결되고 선택 마커의 발현은 세포에 독성이다. 이러한 선택 마커의 비제한적인 예는 잔틴/구아닌 포스포리보실 트랜스퍼라제 (gpt), 하이포잔틴-구아닌 포스포리보실트랜스퍼라제 (HGPRT) 또는 단순 포진 바이러스 티미딘 키나제 (HSV-TK)를 포함한다. 선택 마커를 인코딩하는 폴리뉴클레오티드는 세포에서 활성인 프로모터에 작동가능하게 연결되어 있다.
iii. 표적화 벡터
표적화 벡터는 진핵생물, 비-인간, 포유류, 비-인간 포유류, 인간, 설치류, 마우스, 래트 또는 햄스터 핵산의 DR6 유전자좌로 삽입 핵산을 도입하는 데 사용된다. 표적화 벡터는 삽입 핵산을 포함하고, 상기 삽입 핵산을 플랭킹하는 5' 및 3' 상동성 아암을 추가로 포함한다. 삽입 핵산을 플랭킹하는 상동성 아암은 진핵생물, 비-인간, 포유류, 비-인간 포유류, 인간, 설치류, 마우스, 래트 또는 햄스터 핵산의 표적 DR6 유전자좌 내의 영역에 상응한다. 참고하기 쉽게, 표적화된 게놈 유전자좌 내의 상응하는 동족 게놈 영역은 "표적 부위"로 지칭된다. 예를 들어, 표적화 벡터는 제1 및 제2 표적 부위에 상보적인 제1 및 제2 상동성 아암에 의해 플랭킹된 제1 삽입 핵산을 포함할 수 있다. 이와 같이, 표적화 벡터는 그에 따라 세포의 게놈 내의 상보적인 표적 부위와 상동성 아암 사이에서 일어나는 상동성 재조합 사건을 통해 표적 유전자좌 핵산으로의 삽입 핵산의 통합을 돕는다.
일 실시 형태에서, 진핵생물, 포유류, 비-인간 포유류, 인간, 설치류, 마우스 또는 햄스터 핵산의 표적 유전자좌는, 5' 상동성 아암에 상보적인 제1 핵산 서열 및 3' 상동성 아암에 상보적인 제2 핵산 서열을 포함한다. 일 실시 형태에서, 제1 및 제2 핵산 서열은 5 kb 이상만큼 분리되어 있다. 다른 실시 형태에서, 제1 및 제2 핵산 서열은 1 kb 이상 50 kb 미만만큼 분리되어 있다. 일 실시 형태에서, 제1 및 제2 핵산 서열은 2 kb 이상만큼 분리되어 있다. 일 실시 형태에서, 제1 및 제2 핵산 서열은 3 kb 이상, 4 kb 이상, 5 kb 이상, 6 kb 이상, 7 kb 이상, 8 kb 이상, 9 kb 이상, 10 kb 이상, 15 kb 이상, 20 kb 이상, 30 kb 이상, 40 kb 이상, 또는 50 kb 이상만큼 분리되어 있다. 더욱 추가의 실시 형태에서, 제1 및 제2 핵산 서열은 1 kb 이상 2 kb 미만, 2 kb 이상 3 kb 미만, 4 kb 이상 5 kb 미만, 5 kb 이상 6 kb 미만, 6 kb 이상 7 kb 미만, 7 kb 이상 8 kb 미만, 약 8 kb 이상 9 kb 미만, 9 kb 이상 10 kb 미만, 또는 10 kb 이상 15 kb 미만, 약 15 kb 이상 약 20 kb 미만, 약 20 kb 이상 약 30 kb 미만, 또는 약 40 kb 이상 약 50 kb 미만만큼 분리되어 있다.
표적화 벡터의 상동성 아암은, 예를 들어 적어도 5 내지 10 kb, 5 내지 15 kb, 10 내지 20 kb, 20 내지 30 kb, 30 내지 40 kb, 40 내지 50 kb, 50 내지 60 kb, 60 내지 70 kb, 70 내지 80 kb, 80 내지 90 kb, 90 내지 100 kb, 100 내지 110 kb, 110 내지 120 kb, 120 내지 130 kb, 130 내지 140 kb, 140 내지 150 kb, 150 내지 160 kb, 160 내지 170 kb, 170 내지 180 kb, 180 내지 190 kb, 190 내지 200 kb의 길이 또는 그 이상을 포함하는, 상응하는 표적 서열과의 상동성 재조합 사건을 촉진시키기에 충분한 임의의 길이를 가질 수 있다. 아래에서 더욱 상세하게 서술되는 바와 같이, 대형 표적화 벡터는 더 긴 길이의 표적화 아암을 사용할 수 있다. 구체적인 실시 형태에서, 5' 상동성 아암 및 3' 상동성 아암의 총합은 10 kb 이상이거나, 5' 상동성 아암 및 3' 상동성 아암의 총합은 적어도 약 16 kb 내지 약 100 kb 또는 약 30 kb 내지 약 100 kb이다. 다른 실시 형태에서, LTVEC의 5' 상동성 아암과 3' 상동성 아암의 총합의 크기는 약 10 kb 내지 약 150 kb, 약 10 kb 내지 약 100 kb, 약 10 kb 내지 약 75 kb, 약 20 kb 내지 약 150 kb, 약 20 kb 내지 약 100 kb, 약 20 kb 내지 약 75 kb, 약 30 kb 내지 약 150 kb, 약 30 kb 내지 약 100 kb, 약 30 kb 내지 약 75 kb, 약 40 kb 내지 약 150 kb, 약 40 kb 내지 약 100 kb, 약 40 kb 내지 약 75 kb, 약 50 kb 내지 약 150 kb, 약 50 kb 내지 약 100 kb, 또는 약 50 kb 내지 약 75 kb, 약 10 kb 내지 약 30 kb, 약 20 kb 내지 약 40 kb, 약 40 kb 내지 약 60 kb, 약 60 kb 내지 약 80 kb, 약 80 kb 내지 약 100 kb, 약 100 kb 내지 약 120 kb, 또는 약 120 kb 내지 약 150 kb이다. 일 실시 형태에서, 결실의 크기는 LTVEC의 5' 상동성 아암과 3' 상동성 아암의 총합의 크기와 동일하거나 유사하다.
상동성 아암 및 표적 부위 (즉, 동족 게놈 영역)는, 두 영역이 상동성 재조합 반응에 대한 기질로서 작용하기에 충분한 수준의 서열 동일성을 서로 공유하는 경우, 서로 "상보성" 또는 "상보적"이다. "상동성"이란, 상응하는 또는 "상보적인" 서열과 동일하거나 서열 동일성을 공유하는 DNA 서열을 의미한다. 주어진 표적 부위와, 표적화 벡터 상에서 확인되는 상응하는 상동성 아암 사이의 서열 동일성은 상동성 재조합이 발생하도록 허용하는 임의의 정도의 서열 동일성일 수 있다. 예를 들어, 표적화 벡터 (또는 이의 단편)의 상동성 아암과 표적 부위 (또는 이의 단편)에 의해 공유되는 서열 동일성의 양은 50% 이상, 55% 이상, 60% 이상, 65% 이상, 70% 이상, 75% 이상, 80% 이상, 81% 이상, 82% 이상, 83% 이상, 84% 이상, 85% 이상, 86% 이상, 87% 이상, 88% 이상, 89% 이상, 90% 이상, 91% 이상, 92% 이상, 93% 이상, 94% 이상, 95% 이상, 96% 이상, 97% 이상, 98% 이상, 99% 이상 또는 100% 서열 동일성일 수 있어서, 이들 서열은 상동성 재조합을 거치게 된다. 더욱이, 상동성 아암과 상보적인 표적 부위 사이의 상보적인 상동성 영역은 절단된 인식 부위에서 상동성 재조합을 촉진하기에 충분한 임의의 길이를 가질 수 있다. 예를 들어, 주어진 상동성 아암 및/또는 상보적인 표적 부위는 (예컨대, 본 명세서의 다른 부분에 기재된 LTVEC 벡터에서 기재된 바와 같이) 적어도 5 내지 10 kb, 5 내지 15 kb, 10 내지 20 kb, 20 내지 30 kb, 30 내지 40 kb, 40 내지 50 kb, 50 내지 60 kb, 60 내지 70 kb, 70 내지 80 kb, 80 내지 90 kb, 90 내지 100 kb, 100 내지 110 kb, 110 내지 120 kb, 120 내지 130 kb, 130 내지 140 kb, 140 내지 150 kb, 150 내지 160 kb, 160 내지 170 kb, 170 내지 180 kb, 180 내지 190 kb, 190 내지 200 kb, 200 kb 내지 300 kb 길이 또는 그 이상의 상보적인 상동성 영역을 포함할 수 있어서, 상동성 아암이 세포의 게놈 내의 상응하는 표적 부위와의 상동성 재조합을 거치기에 충분한 상동성을 갖게 된다. 참고하기 쉽게, 상동성 아암은 본 명세서에서 5' 및 3' 상동성 아암으로 지칭된다. 이 용어는 표적화 벡터 내의 삽입 핵산에 대한 상동성 아암의 상대적인 위치에 관한 것이다.
그러므로, 표적화 벡터의 상동성 아암은 표적화된 유전자좌를 갖는 표적 부위에 상보적이도록 설계된다. 따라서, 상동성 아암은 세포에 고유한 유전자좌에 상보적일 수 있거나, 대안적으로 이는 트랜스진, 발현 카세트, 또는 게놈 DNA의 이종성 또는 외인성 영역을 포함하지만 이에 한정되지 않는, 세포의 게놈에 통합된 DNA의 이종성 또는 외인성 분절의 영역에 상보적일 수 있다. 대안적으로, 표적화 벡터의 상동성 아암은 적절한 숙주 세포에 함유된 인간 인공 염색체의 영역 또는 임의의 다른 유전자 조작된 게놈 영역에 상보적일 수 있다. 또한, 표적화 벡터의 상동성 아암은 BAC 라이브러리, 코스미드(cosmid) 라이브러리 또는 P1 파지 라이브러리의 영역에 상보적이거나 이로부터 유래될 수 있다. 따라서, 구체적인 실시 형태에서, 표적화 벡터의 상동성 아암은 주어진 세포에 대해 고유한, 이종성인 또는 외인성인 진핵생물, 비-인간, 포유류, 비-인간 포유류, 인간, 설치류, 마우스 또는 래트 게놈 유전자좌에 상보적이다. 일 실시 형태에서, 상동성 아암은 합성 DNA로부터 유래된다.
표적화 벡터 (예컨대, 대형 표적화 벡터)는 또한 본 명세서의 다른 부분에서 논의된 바와 같은 선택 카세트 또는 리포터 유전자를 포함할 수 있다. 선택 카세트는 선택 마커를 인코딩하는 핵산 서열을 포함할 수 있으며, 여기서 핵산 서열은 본 명세서의 다른 부분에서 논의된 바와 같은 프로모터에 작동가능하게 연결된다. 표적화 벡터의 선택 마커 및/또는 리포터 유전자는 5' 및 3' 상동성 아암에 의해 플랭킹될 수 있거나, 상동성 아암의 5' 또는 3' 중 하나에서 발견될 수 있다.
일 실시 형태에서, 표적화 벡터는, 리포터를 인코딩하는 제1 뉴클레오티드 서열을 포함하는 삽입 핵산을 포함한다. 일부의 경우, DR6 유전자좌와의 상동성 재조합 후, 리포터를 인코딩하는 제1 뉴클레오티드 서열은, DR6 유전자좌에서 DR6의 발현을 유도하는 내인성 프로모터에 작동가능하게 연결된다.
다른 실시 형태에서, 표적화 벡터는, 리포터를 인코딩하는 제2 뉴클레오티드 서열과 작동가능하게 연결된 막 관통 도메인을 인코딩하는 제1 뉴클레오티드 서열을 포함하는 삽입 핵산을 포함한다. 일부의 경우, DR6 유전자좌와의 상동성 재조합 후, 막 관통 도메인을 인코딩하는 제1 뉴클레오티드 서열은, 막 관통 도메인 및 리포터의 목적지를 유도하는 내인성 DR6 신호 서열에 작동가능하게 연결된다.
다른 실시 형태에서, 표적화 벡터의 삽입 핵산은, 선택가능한 마커를 인코딩하는 추가의 뉴클레오티드 서열을 포함한다. 일부의 경우, 추가의 핵산은 프로모터에 작동가능하게 연결된다.
일 실시 형태에서, 삽입 핵산의 제1 및/또는 추가 뉴클레오티드 서열은 Kozak 공통 서열을 포함한다.
일 실시 형태에서, 표적화 벡터 (예를 들어, 대형 표적화 벡터)는 본 명세서의 다른 부분에 기재된 바와 같은 프로모터에 작동가능하게 연결된 리포터 유전자 및/또는 선택가능한 마커 유전자를 포함한다. 이러한 리포터 유전자 및/또는 선택가능한 마커 유전자는 본 명세서의 다른 부분에 기재된 바와 같은 세포에서 활성인 프로모터에 작동가능하게 연결될 수 있다.
일 실시 형태에서, 표적화 벡터는 부위-특이적 재조합효소 유전자를 포함한다. 일 실시 형태에서, 부위-특이적 재조합효소 유전자는 Cre 재조합효소를 인코딩한다. 일 실시 형태에서, Cre 재조합효소 유전자는 Crei이며, Cre 재조합효소를 인코딩하는 2개의 엑손이 인트론에 의해 분리되어, 원핵 세포에서 그의 발현이 억제된다. 일 실시 형태에서, 부위-특이적 재조합효소 유전자는 Dre 재조합효소를 인코딩한다.
일 실시 형태에서, Cre 재조합효소 유전자는 핵에 대한 Cre (또는 임의의 재조합효소 또는 뉴클레아제 제제)의 위치화를 촉진시키기 위한 핵 위치화 신호를 추가로 포함한다 (예를 들어, 상기 유전자는 NLS-Cre 유전자임). 구체적인 실시 형태에서, Cre 재조합효소 유전자는 핵 위치화 신호 (NLS) 및 인트론 (예를 들어, NLS-Crei)을 추가로 포함한다.
다양한 실시 형태에서, 상기에서 논의된 Cre 또는 Crei 재조합효소의 발현에 적합한 프로모터는 Prm1, Blimp1, Gata6, Gata4, Igf2, Lhx2, Lhx5, 및/또는 Pax3으로부터 선택되거나, 그를 포함한다. 구체적인 실시 형태에서, 프로모터는 Gata6 또는 Gata4 프로모터이다. 상기 다양한 프로모터는, 예를 들어, 마우스 또는 래트와 같은 설치류, 진핵생물, 비-인간 포유류, 포유류, 인간 또는 햄스터를 포함한 임의의 유기체로부터 유래된 것일 수 있다. 구체적인 다른 실시형태에서, 프로모터는 Prm1 프로모터이다. 구체적인 다른 실시형태에서, 프로모터는 마우스 Prm1 프로모터이다. 구체적인 다른 실시형태에서, 프로모터는 Blimp1 프로모터 또는 그의 단편, 예를 들어 Blimp1 프로모터의 1 kb 또는 2 kb 단편이다. 예를 들어, 각각 본 명세서에 전체적으로 참고로 포함되는 미국 특허 제8,697,851호, 미국 특허 제9,267,152호, 미국 특허 제9,096,870호, 미국 특허 제8,354,389호, 미국 특허 제8,946,505호, 미국 특허 제8,946,504호, 미국 특허 제8,518,392를 참조한다.
일 실시 형태에서, 삽입 핵산은 2개의 부위-특이적 재조합 부위에 의해 플랭킹된 뉴클레오티드 서열을 포함한다. 부위-특이적 재조합 부위의 예는 loxP, lox511, lox2272, lox66, lox71, loxM2, lox5171, FRT, FRT11, FRT71, attp, att, FRT, rox, 및 이들의 조합을 포함하지만 이에 한정되지 않는다.
iv. 대형 표적화 벡터
용어 "대형 표적화 벡터" 또는 "LTVEC"는 세포에서 상동성 표적화를 수행하도록 의도된 다른 접근법에 의해 전형적으로 사용되는 것보다 큰 핵산 서열에 상응하며 이로부터 유래되는 상동성 아암을 포함하고/하거나 세포에서 상동성 재조합 표적화를 수행하도록 의도된 다른 접근법에 의해 전형적으로 사용되는 것보다 큰 핵산 서열을 포함하는 삽입 뉴클레오티드를 포함하는 대형 표적화 벡터를 포함한다. 구체적인 실시 형태에서, LTVEC의 상동성 아암 및/또는 삽입 폴리뉴클레오티드는 진핵 세포의 게놈 서열을 포함한다. LTVEC의 크기는 너무 커서 종래의 검정, 예를 들어, 서던 블롯팅 및 긴-범위 (예를 들어, 1 kb 내지 5 kb) PCR에 의한 표적화 사건의 스크리닝을 가능하게 할 수 없다. LTVEC의 예는 박테리아 인공 염색체 (BAC), 인간 인공 염색체 또는 효모 인공 염색체 (YAC)로부터 유래된 벡터를 포함하지만 이에 한정되지 않는다. LTVEC의 비제한적인 예 및 이들을 제조하는 방법은 예를 들어, 각각 본 명세서에 참고로 포함된, 미국 특허 제6,586,251호, 제6,596,541호, 제7,105,348호 및 국제 특허 공개 WO 2002/036789호 (PCT/US01/45375호)에 기재되어 있다.
LTVEC는 적어도 약 10 kb, 약 15 kb, 약 20 kb, 약 30 kb, 약 40 kb, 약 50 kb, 약 60 kb, 약 70 kb, 약 80 kb, 약 90 kb, 약 100 kb, 약 150 kb, 약 200 kb, 약 10 kb 내지 약 15 kb, 약 15 kb 내지 약 20 kb, 약 20 kb 내지 약 30 kb, 약 30 kb 내지 약 50 kb, 약 50 kb 내지 약 300 kb, 약 50 kb 내지 약 75 kb, 약 75 kb 내지 약 100 kb, 약 100 kb 내지 125 kb, 약 125 kb 내지 약 150 kb, 약 150 kb 내지 약 175 kb, 약 175 kb 내지 약 200 kb, 약 200 kb 내지 약 225 kb, 약 225 kb 내지 약 250 kb, 약 250 kb 내지 약 275 kb 또는 약 275 kb 내지 약 300 kb를 포함하지만 이에 한정되지 않는 임의의 길이일 수 있다.
일 실시 형태에서, LTVEC의 상동성 아암은 BAC 라이브러리, 코스미드 라이브러리, 또는 P1 파지 라이브러리로부터 유래된다. 다른 실시 형태에서, 상동성 아암은 세포의 표적화된 DR6 게놈 유전자좌로부터 유래되며, 일부 경우에, LTVEC가 표적화하도록 설계된 표적 게놈 유전자좌는 종래의 방법을 사용하여 표적화될 수 없다. 또 다른 실시 형태에서, 상동성 아암은 합성 DNA로부터 유래된다.
일 실시 형태에서, LTVEC의 상류 상동성 아암과 하류 상동성 아암의 총합은 10 kb 이상이다. 다른 실시 형태에서, 상류 상동성 아암은 약 5 kb 내지 약 100 kb 범위이다. 일 실시 형태에서, 하류 상동성 아암은 약 5 kb 내지 약 100 kb 범위이다. 다른 실시 형태에서, 상류 및 하류 상동성 아암의 총합은 약 5 kb 내지 약 10 kb, 약 10 kb 내지 약 20 kb, 약 20 kb 내지 약 30 kb, 약 30 kb 내지 약 40 kb, 약 40 kb 내지 약 50 kb, 약 50 kb 내지 약 60 kb, 약 60 kb 내지 약 70 kb, 약 70 kb 내지 약 80 kb, 약 80 kb 내지 약 90 kb, 약 90 kb 내지 약 100 kb, 약 100 kb 내지 약 110 kb, 약 110 kb 내지 약 120 kb, 약 120 kb 내지 약 130 kb, 약 130 kb 내지 약 140 kb, 약 140 kb 내지 약 150 kb, 약 150 kb 내지 약 160 kb, 약 160 kb 내지 약 170 kb, 약 170 kb 내지 약 180 kb, 약 180 kb 내지 약 190 kb, 또는 약 190 kb 내지 약 200 kb이다. 일 실시 형태에서, 결실의 크기는 LTVEC의 5' 상동성 아암과 3' 상동성 아암의 총합의 크기와 동일하거나 유사하다.
일 실시 형태에서, LTVEC는 본 명세서의 다른 부분에서 논의된 바와 같은 선택 카세트 또는 리포터 유전자를 포함한다.
III. 서열을 도입하여 트랜스제닉 동물을 생산하는 방법
상기에서 개괄적으로 서술된 바와 같이, DR6 유전자좌의 표적화된 유전자 변형을 가능하게 하는 방법 및 조성이 본 명세서에 제공된다. 추가의 표적화된 유전자 변형이 이루어질 수 있다는 것이 추가로 인식된다. 이러한 표적화된 유전자 변형을 가능하게 하는 이러한 시스템은 다양한 성분을 사용할 수 있으며, 참고하기 쉽게, 본 명세서에서 용어 "표적화된 게놈 통합 시스템"은 일반적으로 통합 사건에 요구되는 모든 성분 (즉, 다양한 뉴클레아제 제제, 인식 부위, 삽입 DNA 폴리뉴클레오티드, 표적화 벡터, 표적 게놈 유전자좌 및 관심 폴리뉴클레오티드)을 포함한다.
본 명세서에 제공된 방법은 표적화된 게놈 통합 시스템의 다양한 성분을 포함하는 하나 이상의 폴리뉴클레오티드 또는 폴리펩티드 작제물을 세포에 도입하는 것을 포함한다. "도입"은 서열이 세포 내부에 접근하도록 하는 방식으로 서열 (폴리펩티드 또는 폴리뉴클레오티드)을 세포에 제공하는 것을 의미한다. 본 명세서에 제공된 방법은 폴리뉴클레오티드가 적어도 하나의 세포 내부에 접근하는 것을 제외하고는, 표적화된 게놈 통합 시스템의 임의의 성분을 세포에 도입하는 특정 방법에 의존하지 않는다. 폴리뉴클레오티드를 다양한 세포 유형에 도입하는 방법은 당해 분야에 공지되어 있으며, 안정한 형질감염 방법, 일시적 형질감염 방법 및 바이러스 매개 방법을 포함하지만 이에 한정되지 않는다.
일부 실시 형태에서, 상기 방법 및 조성에 사용되는 세포는 이들의 게놈에 안정하게 혼입된 DNA 작제물을 갖는다. "안정하게 혼입된" 또는 "안정하게 도입된"은, 뉴클레오티드 서열이 세포의 게놈에 통합되고 그의 자손에 의해 유전될 수 있도록 폴리뉴클레오티드를 세포에 도입하는 것을 의미한다. DNA 작제물 또는 표적화된 게놈 통합 시스템의 다양한 성분의 안정한 혼입을 위해 임의의 프로토콜을 사용할 수 있다.
형질감염 프로토콜뿐만 아니라 폴리펩티드 또는 폴리뉴클레오티드 서열을 세포 내로 도입시키는 프로토콜도 다양할 수 있다. 화학 기반 형질감염 방법을 포함한 비제한적인 형질감염 방법은 리포솜; 나노입자; 인산칼슘 (문헌[Graham et al. (1973). Virology 52 (2): 456―67], 문헌[Bacchetti et al. (1977) Proc Natl Acad Sci USA 74 (4): 1590―4] 및 문헌[Kriegler, M (1991). Transfer and Expression: A Laboratory Manual. New York: W. H. Freeman and Company. pp.96―97)]; 덴드리머 (dendrimer); 또는 DEAE-덱스트란 또는 폴리에틸렌이민과 같은 양이온성 중합체의 사용을 포함한다. 비화학적 방법은 전기천공법; 초음파천공법(Sono-poration); 및 광학 형질감염을 포함한다. 입자-기반 형질감염은 유전자 총(gene gun), 자성 보조 형질감염 (문헌[Bertram, J. (2006) Current Pharmaceutical Biotechnology 7, 277―28])의 사용을 포함한다. 바이러스 방법도 형질감염에 사용될 수 있다.
비-인간 동물은 본 명세서에 개시된 다양한 방법을 사용하여 생산될 수 있다. 이러한 방법은 (1) 본 명세서에 개시된 방법을 사용하여, 비-인간 동물의 만능성 세포의 관심 표적 DR6 게놈 유전자좌에 하나 이상의 관심 폴리뉴클레오티드를 통합하여, 표적화된 DR6 게놈 유전자좌에 삽입 폴리뉴클레오티드를 포함하는 유전자 변형된 만능성 세포를 생성하는 단계; (2) 표적 DR6 게놈 유전자좌에 하나 이상의 관심 폴리뉴클레오티드를 가지는 유전자 변형된 만능성 세포를 선택하는 단계; (3) 유전자 변형된 만능성 세포를, 예를 들어 상실기 이전 단계의 비-인간 동물의 숙주 배아에 도입하는 단계; 및 (4) 유전자 변형된 만능성 세포를 포함하는 숙주 배아를 대리모에 이식하여, 유전자 변형된 만능성 세포로부터 유래된 F0 세대를 생산하는 단계를 포함한다. 챌린징 표적 염색체 유전자좌를 표적화하도록 유사한 방법이 사용될 수 있다. 비-인간 동물은 비-인간 포유류, 설치류, 마우스, 래트, 햄스터, 원숭이, 농업용 포유류 또는 사육용 포유류, 또는 어류 또는 조류일 수 있다.
만능성 세포는 인간 ES 세포, 비-인간 ES 세포, 설치류 ES 세포, 마우스 ES 세포, 래트 ES 세포, 햄스터 ES 세포, 원숭이 ES 세포, 농업용 포유류 ES 세포 또는 사육된 포유류 ES 세포일 수 있다. 다른 실시 형태에서, 만능성 세포는 비-인간 세포, 포유류 세포, 인간 세포, 비-인간 포유류 세포, 인간 만능성 세포, 인간 ES 세포, 인간 성인 줄기 세포, 발생적으로 제한된 인간 전구 세포, 인간 iPS 세포, 설치류 세포, 래트 세포, 마우스 세포, 햄스터 세포이다. 일 실시 형태에서, 표적화된 유전자 변형은 DR6의 기능 상실을 야기한다.
마우스 만능성 세포, 전능성 세포 또는 숙주 배아는, 예를 들어, 근친 교배 계통, 잡종 계통, 및 이계 교배 계통을 포함하는 임의의 마우스 계통으로부터 유래된 것일 수 있다. 마우스 계통의 예는 129 계통, C57BL 계통 (예를 들어, C57BL/6 계통), 129 및 C57BL/6의 혼합 (예를 들어, 50% 129 및 50% C57BL/6), BALB/c 계통, 및 스위스 웹스터(Swiss Webster) 계통을 포함한다. 129 계통의 예는 129P1, 129P2, 129P3, 129X1, 129S1 (예를 들어, 12951/SV, 12951/SvIm), 129S2, 129S4, 129S5, 12959/SvEvH, 129S6 (129/SvEvTac), 129S7, 129S8, 129T1, 및 129T2를 포함한다 (예를 들어, 문헌 [Festing et al. (1999) Revised nomenclature for strain 129 mice, Mammalian Genome 10:836] 참조). C57BL 계통의 예는 C57BL/A, C57BL/An, C57BL/GrFa, C57BL/KaLwN, C57BL/6, C57BL/6J, C57BL/6ByJ, C57BL/6NJ, C57BL/10, C57BL/10ScSn, C57BL/10Cr, 및 C57BL/01a를 포함한다. 마우스는 전술한 129 계통 (예를 들어, 129S6 (129/SvEvTac) 계통) 및 전술한 C57BL/6 계통의 혼합물, 하나 이상의 전술한 129 계통의 혼합물, 또는 하나 이상의 전술한 C57BL 계통의 혼합물일 수 있다. 마우스는 또한 129 계통을 제외한 계통으로부터 유래된 것일 수 있다.
래트 만능성 세포, 전능성 세포 또는 숙주 배아는, 예를 들어 근친 교배 계통, 잡종 계통, 및 이계 교배 계통을 포함하는 임의의 래트 계통으로부터 유래된 것일 수 있다. 래트 계통의 예는 ACI 래트 계통, 다크 아구티(Dark Agouti; DA) 래트 계통, 위스타(Wistar) 래트 계통, LEA 래트 계통, 스프라그 다울리(Sprague Dawley; SD) 래트 계통, 또는 피셔(Fischer) 래트 계통, 예를 들어 피셔 F344 또는 피셔 F6을 포함한다. 래트 만능성 세포, 전능성 세포 또는 숙주 배아는 또한 상기에 열거된 둘 이상의 계통의 혼합체로부터 유래되는 계통으로부터 얻어질 수 있다. 예를 들어, 래트 만능성 세포, 전능성 세포 또는 숙주 배아는 DA 계통 및 ACI 계통으로부터 선택되는 계통으로부터 유래될 수 있다. ACI 래트 계통은 블랙 아구티(black agouti)를 가지며 배와 발이 흰색이고 RT1 av1 하플로타입(haplotype)을 갖는 것을 특징으로 한다. 이러한 계통은 할란 래보러토리즈(Harlan Laboratories)를 포함하는 다양한 공급처로부터 입수가능하다. ACI 래트 유래의 래트 ES 세포주의 예는 ACI.G1 래트 ES 세포이다. 다크 아구티 (DA) 래트 계통은 아구티 털(agouti coat) 및RT1 av1 하플로타입을 갖는 것을 특징으로 한다. 이러한 래트는 찰스 리버 래보러토리즈(Charles River Laboratories) 및 할란 래보러토리즈를 포함하는 다양한 공급처로부터 입수가능하다. DA 래트 유래의 래트 ES 세포주의 예는 DA.2B 래트 ES 세포주 또는 DA.2C 래트 ES 세포주이다. 래트 계통의 다른 예는, 예를 들어, 각각 모든 목적을 위하여 본 명세서에 전체적으로 참고로 포함된 미국 특허 출원 공개 제2014/0235933호, 미국 특허 출원 공개 제2014/0310828호, 및 미국 특허 출원 공개 제2014/0309487호에 제공되어 있다.
예를 들어, N2 보충제, B27 보충제, 약 50 U/mL 내지 약 150 U/mL의 백혈병 저해 인자 (LIF) 및 MEK 저해제 및 GSK3 저해제로 이루어진 저해제의 조합을 포함하는 배지를 사용하여, 단리된 래트 ES 세포를 피더 세포 층 상에서 배양함으로써, 생식세포계열-전달가능한 래트 ES 세포를 얻을 수 있고, 상기 피더 세포 층은 LIF를 발현하도록 변형되지 않으며, 래트 ES 세포는 (i) 래트 ES 세포의 게놈 내로의 선택 마커를 포함한 이종성 폴리뉴클레오티드의 적어도 하나의 삽입을 포함하는 표적화된 유전자 변형을 포함하도록 변형되었으며, 생식세포계열을 통해 상기 표적화된 유전자 변형을 전달할 수 있고; (ii) 정상 핵형을 가지며; (iii) c-Myc의 발현이 결여되어 있고; (iv) 배양 시에 구형의 자유-유동 콜로니를 형성한다 (예를 들어, 각각 전체적으로 참고로 포함된 미국 특허 출원 공개 제2014-0235933 A1호 및 미국 특허 출원 공개 제2014-0310828 A1호 참조). 래트 배아 줄기 세포의 유도 및 표적화된 변형의 다른 예는, 예를 들어, 문헌[Yamamoto et al. ("Derivation of rat embryonic stem cells and generation of protease-activated receptor-2 knockout rats," Transgenic Res. 21:743-755, 2012)] 및 문헌[Kwamata and Ochiya ("Generation of genetically modified rats from embryonic stem cells," Proc. Natl. Acad. Sci. USA 107(32):14223-14228, 2010)]에 제공되어 있다.
또한, 핵 전달 기법을 사용하여 비-인간 동물을 생산할 수 있다. 간략히 말해서, 핵 전달을 위한 방법은 하기의 단계를 포함한다: (1) 난모세포를 제핵시키는 단계; (2) 제핵된 난모세포와 조합될 공여자 세포 또는 핵을 단리하는 단계; (3) 제핵된 난모세포 내로 상기 세포 또는 핵을 삽입하여, 재구성된 세포를 형성하는 단계; (4) 재구성된 세포를 동물의 자궁에 이식하여 배아를 형성하는 단계; 및 (5) 배아가 발생하도록 하는 단계. 이러한 방법에서, 난모세포는 일반적으로 사망한 동물로부터 회수되지만, 살아있는 동물의 난관 및/또는 난소로부터도 단리될 수 있다. 난모세포는 제핵 전에 당업자에게 공지된 다양한 배지에서 성숙될 수 있다. 난모세포의 제핵은 당업자에게 잘 알려진 다수의 방식으로 수행할 수 있다. 공여자 세포 또는 핵을 제핵된 난모세포 내로 삽입하여 재구성된 세포를 형성하는 것은 보통 융합 전에 투명대 (zona pellucida) 아래로의 공여자 세포의 미세주입 (microinjection)에 의한다. 융합은 접촉면/융합면(전기융합)을 가로질러 DC 전기 펄스를 인가함으로써, 폴리에틸렌 글리콜과 같은 융합 촉진 화학 물질에 세포를 노출시킴으로써, 또는 센다이 바이러스(Sendai virus)와 같은 비활성화 바이러스에 의해 유도될 수 있다. 재구성된 세포는 전형적으로 핵 공여자 및 수용자 난모세포의 융합 전, 융합 동안, 및/또는 융합 후 전기적 및/또는 비-전기적 수단에 의해 활성화된다. 활성화 방법은 전기 펄스, 화학적으로 유도된 충격, 정자에 의한 침투, 난모세포에서의 2가 양이온 수준의 증가, 및 난모세포에서의 세포 단백질의 인산화 감소 (키나제 저해제에 의한 바와 같음)를 포함한다. 활성화된 재구성된 세포, 또는 배아는 전형적으로 당업자에게 잘 알려진 배지에서 배양되고 이어서 동물의 자궁으로 전달된다. 예를 들어, 각각 본 명세서에 참고로 포함된, 미국 특허 출원 공개 제20080092249호, 국제 특허 공개 WO/1999/005266A2호, 미국 특허 출원 공개 제20040177390호, 국제 특허 공개 WO/2008/017234A1호 및 미국 특허 제7,612,250호를 참조한다.
하기의 단계를 포함하는 것으로서, 본 명세서에 기재된 바와 같은 생식세포계열의 하나 이상의 유전자 변형을 포함하는 비-인간 동물을 제조하는 다른 방법이 제공된다: (a) 본 명세서에 기재된 다양한 방법을 사용하여, 원핵 세포에서 비-인간 동물의 표적화된 게놈 DR6 유전자좌를 변형시키는 단계; (b) 표적화된 게놈 유전자좌에서 유전자 변형을 포함하는 변형된 원핵 세포를 선택하는 단계; (c) 변형된 원핵 세포의 게놈으로부터 유전자 변형된 표적화 벡터를 단리하는 단계; (d) 유전자 변형된 표적화 벡터를 비-인간 동물의 만능성 세포에 도입하여, 표적화된 DR6 게놈 유전자좌에서 삽입 핵산을 포함하는 유전자 변형된 만능성 세포를 생성하는 단계; (e) 유전자 변형된 만능성 세포를 선택하는 단계; (f) 유전자 변형된 만능성 세포를 상실기 이전 단계에서 비-인간 동물의 숙주 배아에 도입하는 단계; 및 (g) 유전자 변형된 만능성 세포를 포함하는 숙주 배아를 대리모에 이식하여, 유전자 변형된 만능성 세포로부터 유래된 F0 세대를 생산하는 단계. 그러한 방법에서, 표적화 벡터는 대형 표적화 벡터를 포함할 수 있다. 비-인간 동물은 비-인간 포유류, 설치류, 마우스, 래트, 햄스터, 원숭이, 농업용 포유류 또는 사육용 포유류일 수 있다. 만능성 세포는 인간 ES 세포, 비-인간 ES 세포, 설치류 ES 세포, 마우스 ES 세포, 래트 ES 세포, 햄스터 ES 세포, 원숭이 ES 세포, 농업용 포유류 ES 세포 또는 사육용 포유류 ES 세포일 수 있다. 다른 실시 형태에서, 만능성 세포는 비-인간 세포, 포유류 세포, 인간 세포, 비-인간 포유류 세포, 인간 만능성 세포, 인간 ES 세포, 인간 성인 줄기 세포, 발생적으로 제한된 인간 전구 세포, 인간 iPS 세포, 인간 세포, 설치류 세포, 래트 세포, 마우스 세포, 햄스터 세포이다. 일 실시 형태에서, 표적화된 유전자 변형은 DR6의 기능 상실을 야기한다.
추가의 방법에서, 단리하는 단계 (c)는 (c1) 유전자 변형된 표적화 벡터 (즉, 유전자 변형된 LTVEC)를 선형화하는 단계를 추가로 포함한다. 추가의 실시 형태에서, 도입 단계 (d)는 (d1) 상동성 재조합을 촉진하기 위해 만능성 세포에 뉴클레아제 제제를 도입하는 단계를 추가로 포함한다. 일 실시 형태에서, 선택 단계 (b) 및/또는 (e)는 본 명세서에 기재된 바와 같은 선택가능한 제제를 원핵 세포 또는 만능성 세포에 적용함으로써 수행된다. 일 실시 형태에서, 선택 단계 (b) 및/또는 (e)는 본 명세서에 기재된 바와 같은 대립유전자의 변형(MOA) 검정을 통해 수행된다.
일부 실시 형태에서, 본 명세서에 기재된 표적 게놈 유전자좌의 다양한 유전자 변형은 VELOCIGENE® 유전자 공학 기술을 사용하여 박테리아 인공 염색체(BAC) DNA로부터 유래된 LTVEC를 사용한 박테리아 세포에서의 일련의 상동성 재조합 반응(BHR)에 의해 수행될 수 있다 (예를 들어, 본 명세서에 전체적으로 참고로 포함된, 미국 특허 제6,586,251호 및 문헌[Valenzuela, D. M. et al. (2003), Nature Biotechnology 21(6): 652-659] 참조).
일부 실시 형태에서, 본 명세서에 기재된 바와 같은 다양한 유전자 변형을 포함하는 DR6 표적화된 만능성 및/또는 전능성 세포는 VELOCIMOUSE® 방법을 통해, 삽입 공여자 세포로서 사용되며, 상응하는 유기체 유래의 상실기 이전 단계의 배아, 예를 들어 8-세포기 마우스 배아로 도입된다 (예를 들어, 모두 본 명세서에 전체적으로 참고로 포함된 미국 특허 제7,576,259호, 미국 특허 제7,659,442호, 미국 특허 제7,294,754호 및 미국 특허 출원 제2008-0078000 A1호 참조). 유전자 변형된 만능성 및/또는 전능성 세포를 포함하는 비-인간 동물 배아는 배반포기 (blastocyst stage)까지 인큐베이션된 다음에, 대리모에 이식되어, F0 세대를 생산한다. 일부 실시 형태에서, 본 명세서에 기재된 바와 같은 다양한 유전자 변형을 포함하는 표적화된 포유류 ES 세포는 배반포기 배아에 도입된다. 유전자 변형된 게놈 유전자좌 (즉, DR6 유전자좌)를 가지는 비-인간 동물은 본 명세서에 기재된 바와 같은 대립유전자의 변형 (MOA) 검정을 통해 확인될 수 있다. 유전자 변형된 만능성 및/또는 전능성 세포로부터 유래된 생성된 F0 세대 비-인간 동물을 야생형 비-인간 동물과 교배시켜 F1 세대 자손을 얻는다. 특정 프라이머 및/또는 프로브를 사용하여 유전자형을 결정한 후, 유전자 변형된 게놈 유전자좌에서 이형접합성인 F1 비-인간 동물을 서로 교배시켜, 유전자 변형된 게놈 유전자좌에서 동형접합성인 F2 세대 비-인간 동물 자손을 생산한다.
일 실시 형태에서, 적어도 하나의 DR6 유전자좌에서 유전자 변형을 포함하는 비-인간 동물을 제조하는 방법이 제공된다. 이러한 방법은 하기의 단계를 포함한다: (a) 만능성 세포를 5' 및 3' 상동성 아암에 의해 플랭킹된 삽입 핵산을 포함하는 표적화 작제물과 접촉시키는 단계로서, 상기 표적화 작제물은 상기 세포의 게놈에서 DR6 유전자좌와 상동성 재조합을 거쳐서, 변형된 만능성 세포를 형성하는, 단계; (b) 변형된 만능성 세포를 숙주 배아에 도입하는 단계; 및 (c) 숙주 배아를 대리모에 임신시키는 단계로서, 상기 대리모는 변형 DR6 유전자좌를 포함하는 자손을 생산하고, 상기 유전자 변형은 적어도 하나의 DR6의 기능 상실을 야기하는, 단계.
IV. 세포
본 명세서에 기재된 다양한 방법은 세포에서 DR6 유전자좌를 변형시키기 위해 게놈 유전자좌 표적화 시스템을 사용한다. 그러한 세포는 원핵 세포, 예컨대 이. 콜라이를 포함하는 박테리아 세포, 또는 진핵 세포, 예컨대 효모, 곤충, 양서류, 식물, 또는 마우스 세포, 래트 세포, 햄스터 세포, 토끼 세포, 돼지 세포, 소 세포, 사슴 세포, 양 세포, 염소 세포, 닭 세포, 고양이 세포, 개 세포, 페럿 세포, 영장류 (예를 들어, 마모셋, 붉은털 원숭이) 세포 등 및 사육된 포유류로부터의 세포 또는 농업용 포유류로부터의 세포를 포함하지만 이에 한정되지는 않는 포유류 세포를 포함한다. 일부 세포는 비-인간, 특히 비-인간 포유류 세포이다. 일부 실시 형태에서, 적합한 유전자 변형가능한 만능성 세포가 용이하게 입수가능하지 않는 포유류의 경우, 예를 들어 Oct3/4, Sox2, KLF4, Myc, Nanog, LIN28 및 Glis1을 포함하지만 이에 한정되지 않는 만능성 유도 인자의 조합을 체세포에 도입하는 것을 통해, 체세포를 만능성 세포로 다시 프로그래밍하기 위한 다른 방법이 사용된다. 이러한 방법에서, 세포는 또한 포유류 세포, 인간 세포, 비-인간 포유류 세포, 비-인간 세포, 설치류, 래트, 마우스, 햄스터 유래의 세포, 섬유아세포 또는 임의의 다른 숙주 세포일 수 있다. 다른 실시 형태에서, 세포는 만능성 세포, 유도 만능성 줄기(iPS) 세포, 비-인간 배아 줄기(ES) 세포이다. 이러한 세포는, 예를 들어, 유도 만능성 줄기(iPS) 세포, 인간 iPS 세포, 마우스 배아 줄기(ES) 세포, 래트 배아 줄기(ES) 세포, 인간 배아 줄기(ES) 세포 또는 발생적으로 제한된 인간 전구 세포, 설치류 배아 줄기(ES) 세포, 마우스 배아 줄기(ES) 세포 또는 래트 배아 줄기(ES) 세포를 포함하는, 만능성 세포를 포함한다.
V. 동물 모델
또한, ALS를 치료, 억제 및/또는 저해하기 위한 후보 제제를 확인하는 방법이 본 명세서에 제공된다. 구체적인 실시 형태에서, 상기 물질의 저해 효과는, DR6 기능 상실을 가지고, 출생시 표현형은 정상이며, 8주령 후 ALS 유사 증상이 발생하는 동물에 상기 제제를 투여함으로써, 생체 내에서 결정된다.
임의의 편리한 경로, 예를 들어 전신 주입, 장기간 노출용 펌프 또는 직접적인 뇌내 주입에 의해, 시험될 제제를 상기 동물에 투여할 수 있다. 이들 동물을 거동 연구에 포함시켜, 상기 제제가 제공되지 않은 적절한 대조군 동물과 비교하여, 동물의 거동, 예를 들어 운동 거동에 대한 상기 물질의 영향을 결정할 수 있다. 동물 척수, 근육 및/또는 뇌 조직의 생검 또는 해부학적 평가를 또한 수행할 수 있고/있거나, 혈액 또는 CSF의 샘플을 수집할 수 있다.
본 발명은 다수의 실시 형태를 참조하여 특별히 나타내어지고 기재되었으나, 당업자는, 본 발명의 사상 및 범주를 벗어나지 않으면서, 본 명세서에 개시된 다양한 실시 형태에 대한 형태 및 세부 사항의 변경이 이루어질 수 있으며, 본 명세서에 개시된 다양한 실시 형태는 청구범위의 범주를 제한하는 것으로 의도되지 않음을 이해할 것이다.
실시예
하기 실시예는 단지 예시를 목적으로 제공되며, 본 발명의 범주를 제한하는 것으로 의도되지 않는다.
실시예 1: DR6 ( Tnfrs21) 유전자좌의 유전자 변형
마우스에서 맞춤 유전자 돌연변이의 신속한 고처리율의 생성을 가능하게 하는 VelociGene® 방법을 전술한 바와 같이 사용하였다 (문헌[Valenzuela, D.M., et al. (2003b), Nat Biotechnol 21:652-659]). 간략히 말해서, 마우스 bMQ (129S7/SvEv Brd-Hprt b-m2) 또는 RP23 BAC 라이브러리 (문헌[Adams, D.J., et al. (2005), Genomics 86:753-758])로부터의 BAC 클론을 사용하여, 대형 표적화 벡터 (LTVEC)를 생성하였다. lacZ/neo r 리포터/선택 카세트 (도 1)는, lacZ 부분의 β-갈락토시다제 코딩 서열의 아미노 말단이 ROR1로부터의 막 관통 도메인을 포함하도록 변형된 것을 제외하고는, NIH KOMP (World Wide Web (www)의 URL "www.VelociGene.com/komp/detail/10020"에서 인터넷을 통해 입수가능한 서열)에 사용된 ZEN-Ub1 카세트와 동일하였다.
다중 웰 전기천공 장치 (미국 매사추세츠주 보스턴 소재의 Harvard Apparatu)를 사용하여, 전기천공 완충액 (Millipore)에서 0.125 ml의 부피 중 0.67 ㎍ DNA, 3.3 × 106 세포로 LTVEC를 C57BL/6NTac ES 세포 (문헌[Poueymirou et al. (2007), Valenzuela et al. (2003a)])로 도입한 후, 15 cm의 젤라틴화된 플레이트에서 배양하였다. 전기천공법 후 48시간 후에 G418을 함유하는 선택 배지를 첨가하고, 그 후에 매일 교체하였다. 약물-내성 콜로니를 전기천공법 후 10일 후에 떼어 내어, 트립신으로 처리하고, DNA 추출 및 정제 전에 3일 이상 동안 젤라틴화된 96-웰 플레이트에서 배양하였다. 대립유전자 변형 (MOA) 검정 (문헌[Frendewey et al. (2010), Methods in enzymology 476:295-307]; 문헌[Valenzuela et al. (2003a), Nat. Biotechnol. 21:652-659])에 의해 정확하게 표적화된 ES 세포 클론을 확인하였다.
VelociMouse® 방법 (문헌[Dechiara, T.M., (2009), Methods Mol Biol 530:311-324]; 문헌[Poueymirou et al. (2007), Nat. Biotechnol. 25:91-99])을 사용하여, 표적화된 ES 세포를 조밀하지 않은 8-세포기의 스위스 웹스터 배아에 주입하고, DR6 녹아웃 돌연변이를 지닌 완전히 ES 세포-유래된 F0 세대 마우스를 생성하였다. lacZ 발현 프로파일링을 위해, 수컷 VelociMouse®를 직접 사용하거나, 또는 C57BL/129 암컷과 교미시켜, lacZ 분석을 위한 배아 또는 성체를 생성하거나, F1 증식자 (breeder)를 생성하고, N2F1 마우스에서 표현형 연구를 수행하였다. 질전 (vaginal plug)을 확인한 날 아침을 0.5일 (E0.5)로 지정함으로써, 시간 지정 교미를 수행하였다.
실시예 2: DR6 유전자좌에 돌연변이를 포함하는 동물의 표현형 분석
N2F1 마우스의 표현형 연구는 6 내지 8주령에 개시하였다. 시간 지정 교미를 위해, 본 발명자들은 질전을 확인한 날 아침을 배아 0.5일 (E0.5)로 지정하였다. DR6 KO 및 야생형 한배 새끼들을 다양한 발달 지표 (발육 부전 (runting), 호흡, 얼굴 및 사지 이상, 피부색, 자세, 정위, 및 눈 뜨기)에 대해, 출생 시로부터 약 6 내지 8주령까지 관찰하였으며, 이때, 동물들은 연구 과정 동안 69 내지 74℉, 및 40 내지 60% 습도에서 하루에 12시간 동안 빛이 제공되는 곳에서 수용하였다. 모든 실험은 6 내지 9주령에 개시하였으며, 모든 동물 실험 절차는 리제너론 파마슈티칼스 기관 동물 관리 및 사용 위원회(Regeneron Pharmaceuticals Institutional Animal Care and Use Committee)에 의해 승인된 프로토콜을 준수하여 수행하였다.
운동 손상 분석은 로타로드 테스트, 오픈 필드 보행성 운동 테스트 및 캣워크 테스트를 사용하여 수행하였다. 캣워크 테스트 시에는, 대상이 조명된 유리 플랫폼을 가로질러 걸어 다니는 동안, 아래로부터 비디오 카메라로 녹화한다. 걸음걸이 관련 파라미터-예를 들어, 보폭 패턴, 각 발의 유각 속도, 입각 시간 및 압력-를 각 동물에 대해 보고한다. 이 테스트를 사용하여, 트랜스제닉 계통의 마우스의 표현형을 결정하고, 운동 수행 능력에 대한 그의 효과에 대해 신규의 화학 물질을 평가한다. 캣워크 XT는 래트와 마우스의 발소리 (footfall)와 걸음걸이를 정량적으로 평가하는 시스템이다. 이것은 중추 신경계, 말초 신경계, 근육계 또는 골격계 이상의 거의 모든 종류의 실험 모델에서 설치류의 보행성 운동 능력을 평가하는 데 사용된다.
상위 운동 뉴런 손상은 경직 (즉, 강직), 반사의 증가, 진전, 운동완만증 및 바빈스키 징후로 나타난다. 하위 운동 뉴런 손상은 근육 약화, 소모, 움켜쥐기, 구부러짐 및 발 끌기 및 근섬유다발수축으로 나타난다. 연수 손상은 연하 곤란, 불분명한 발음 및 혀의 근섬유다발수축으로 나타나다. 표 2는 테스트 중 동물의 운동 손상, 진전 및 강직과 관련된 점수화 방법론을 제공한다. 블라인드 주관적 점수화 검정을 사용하여 전반적인 운동 기능 평가를 수행하였으며, 모든 데이터는 평균+/- SEM으로 보고되어 있다.
[표 2]
Figure pat00003
데이터는, DR6-/- 마우스가 그의 야생형 상대와 비교하여 부동 상태가 증가하는 것으로 보이지는 않으나, 로타로드 위에서의 시간 및 중량의 차이가 9주령만큼 일찍 나타날 수 있고, 이의 각각은 시간 경과에 따라 꾸준히 저하됨에도 불구하고, 대략 14주령에 ALS 유사 신경학적 표현형 (예를 들어, 뒷다리 불완전 마비를 시사하는 적은 양육 활동)을 나타냄을 보여준다 (도 4 내지 도 7). 그러나, DR6-/- 마우스의 운동 뉴런 상실은 운동 손상, 강직 및 진전 점수가 마비 및/또는 중증 질환 진행을 반영하기 시작하는 약 14주령에 임상 증상에 대한 한계점에 도달하며, 이는 동물의 성별에 무관하다 (도 2). 증상이 나타난 DR6-/- 동물은 상위 및 하위 운동 뉴런 손상 둘 모두를 반영하는 중량 감소 및 유의한 운동 이상을 가지며 (도 3), 약 21주령에 사망한다.
실시예 3: DR6 유전자좌에 돌연변이를 포함하는 동물의 해부학적 분석
약 20주령의 8마리의 야생형 및 8마리의 DR6 녹아웃 마우스를 50 mL의 식염수로 관류시킨 후에, pH 6.5의 아세트산염 완충액 중 4% 파라포름알데하이드 (PFA) 용액 50 mL와 pH 9.5의 붕산염 완충액 중 4% PFA 용액 50 ml로 관류시켰다. 동물의 관류 후, 마우스의 뇌와 척수를 수집하여, 이들이 떨어질 때까지, 붕산염 완충액 중 15% 수크로스 용액 후에, 30% 수크로스 용액에 넣는다. 그 다음, 조직을 OCT에 포매하고, 2-메틸부탄을 사용하여 동결시켰다.
동결된 조직을 잘라내어, 슬라이드에 올려놓고, 헤마톡실린과 에오신 (H&E) 및 룩솔 패스트 블루 (Luxol fast blue)로 면역염색하였다. 척수 당 4개의 H&E 염색된 슬라이드를 무작위로 선택하여, 각각의 척수의 운동 뉴런의 수를 계수하였다.
대표 슬라이드가 도 9에 제공되어 있다. 야생형 마우스의 척수에서 슬라이드당 평균 운동 뉴런의 수는 약 18개이며, 녹아웃 마우스의 경우에는 약 2개이고, p 값은 0.0001 미만이다 (도 9). 이는, 증상이 나타난 DR6 녹아웃 마우스의 경우에 그들의 야생형 상대와 비교하여 척수에서 운동 뉴런이 유의하게 상실됨을 보여준다. 또한, 녹아웃 마우스의 척수는, 크로이츠펠트야콥병 환자의 인간 뇌 병리와 닮은 해면모양 병리 (spongiform pathology)를 나타내며, 이는 이들 동물에서의 프리온-유사 운동 뉴런 질환 표현형의 가능성을 시사한다.
실시예 4: DR6 유전자좌에 돌연변이를 포함하는 동물에 의한 뉴런 단백질의 발현
약 20주령의 10마리의 야생형 및 7마리의 DR6 녹아웃 마우스의 뇌와 척수로부터 전체 mRNA를 수집하였다. 그 다음, 이 mRNA 샘플에 대해 Taqman®을 이용한 RT-PCR을 수행하였다. 이어서, 마이엘린 결합 단백질 (MBP), 신경생장인자수용체 (NGFR), 콜린 아세틸트랜스퍼라제 (Chat), Mnx 호메오박스, 글루타메이트 [NMDA] 수용체 하위 단위 3B (Grin3b), 및 글루타메이트 수용체 2(Gria2) 유전자의 평균 mRNA 수준을 샘플의 β 액틴의 발현에 의해 정규화하였다.
도 10 내지 도 12는, DR6 녹아웃 마우스의 척수에서 MBP, Mnx 및 Grin3b의 발현이 감소하였으나, 뇌에서는 그러하지 않음을 보여주며, 이는 증상이 나타난 DR6 녹아웃 마우스의 척수에서 운동 뉴런 상실에 대한 증거를 보강하는 것이다. Ngfr, Chat 및 Gri2a의 발현은 영향을 받지 않는다.
실시예 5: 내인성 DR6 유전자좌에 돌연변이를 포함하는 동물의 유전자 발현 패턴
10주령의 야생형 마우스 (n=5 수컷), 증상이 나타나기 전의 10주령의 DR6-/- 마우스 (n=5 수컷), 20주령의 야생형 마우스 (n=6 수컷 및 3 암컷), 및 증상이 나타난 20주령의 DR6-/- 마우스 (n=6 수컷 및 3 암컷)의 뇌와 척수 조직으로부터 추출한 RNA를 사용하여, 유전자 프로파일링을 수행하였다.
10주령 및 20주령의 DR6-/- 마우스의 뇌와 척수는 유전자 발현이 상당히 중첩되고, 유사한 유전자 발현 특징을 공유한다 (데이터는 제시되지 않음). 10주령의 DR6-/- 마우스의 뇌와 척수 둘 모두에서 주요 유전자 73개가 질환의 기전과 진행에 잠재적으로 관여하는 것으로 확인되었다. 10주령의 DR6-/- 마우스의 척수의 유전자 특징은 또한 인간 ALS 및 쥣과 동물 SOD1 바이오세트와 고도로 상호 연계되며 (데이터는 제시되지 않음), 이는 DR6-/- 마우스를 ALS 마우스 모델로서 추가로 확인시켜 주는 것이다. 10주령의 DR6-/- 마우스의 뇌와 척수는 면역 반응과 연결된 유전자 발현 특징을 나타내었으며, 이는 상기 질환 과정이 염증 경로와 관련되어 있음을 시사한다.
실시예 6: DR6-/- 동물은 말초 면역 세포의 항상성을 나타낸다.
CD3, B220, CD21 및 CD2에 대한 항체 (모두 미국 캘리포니아주 새너제이 소재의 BD Biosciences 또는 미국 캘리포니아주 샌디에고 소재의 eBioscience로부터 입수하였음)를 사용하여, 유세포계측 분석에 의해 표현형을 결정하기 위해, 10주령 및 20주령의 야생형 및 DR-/- 마우스로부터 혈액, 골수, 흉선 및 비장 조직을 단리하였다. 또한, 20주령의 야생형 및 DR6-/- 마우스로부터 단리된 혈청의 사이토카인 및 케모카인 농도를, FGF 기반, GM--CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, IL-12 (p40/p70), IL-17, IP-10, KC, MCP-1, MIG, MIP-1α, TNF-α, 및 VEGF의 동시 정량 결정을 위해, 루미넥스 마우스 사이토카인 마그네틱 20-플렉스 패널(Luminex Mouse Cytokine Magnetic 20-Plex Panel)을 사용하여 평가하였다.
10주령 또는 20주령의 DR6-/- 마우스 모두는 말초 면역 세포 항상성에 대해 강력한 효과를 나타내지 않았다 (도 13 내지 도 14). DR6-/- 마우스는 골수에서 정상적인 B 세포 발생 및 초기 세포 전구 세포 항상성, 흉선에서 B 세포의 2배 증가, 비장 이행 1 B 세포의 증가 및 비장 변연부 B 세포의 감소, 말초 호중구의 감소, 및 말초 림프구의 증가를 나타내었다 (도 13). 골수에서, DR6-/- 마우스는 거핵구/적혈구 전구 세포의 14% 증가, 골수 전구 세포의 20% 감소를 나타내었으나, 총 세포 수의 유의한 변화는 없었다 (도 14). DR6-/- 동물은 흉선에서 정상적인 T 세포 발생 및 분화를 나타내었고, 비장 공통/성숙 수상돌기 세포의 20% 증가, 대식세포 및 호산구의 25% 증가, 비장의 호중구 및 단핵구의 75% 증가, 및 CD4+ T 세포의 19% 감소가 있었다. 그러나, 비장에서 총 세포 수의 유의한 변화는 없었다 (데이터는 제시되지 않음). 또한, 20주령의 DR6-/- 마우스의 혈청의 사이토카인과 케모카인 수준은 야생형 동물에 비해 변화하지 않았다 (도 15). 이 데이터는, DR6-/- 마우스가 질환 진행 중에 전반적인 염증 반응을 거치지 않음을 나타내며, 유전적 프로파일링으로 나타나는 염증 반응이 국소적 신경 염증일 수 있음을 시사한다.
실시예 7: DR6+/- 마우스의 배아 줄기 세포 유래 운동 뉴런은 산화 스트레스의 증가를 나타낸다.
야생형 (DR6+/+) 또는 이형접합 DR6+/- 동물로부터 내세포집단 배아 줄기 세포를 단리하고, 배아 줄기 세포 배지 (ESM; DMEM + 15% 소 태아 혈청 + 페니실린/스트렙토마이신 + 글루타민 + 비 필수 아미노산 + 뉴클레오시드 + β-메르캅토에탄올 + 피루브산나트륨 + LIF)에서 2일 동안 배양하여, 배상체 (EB)를 형성하였다. EB를 분화 배지 (DM: 최신 DMEM/F12 + 뉴런세포 기본 배지 + 10% 녹아웃 혈청 + 페니실린/스트렙토마이신 + 글루타민 + β-메르캅토에탄올)에서 2일 동안 배양하였고, DM과 레티노산과 평활화 작동제에서 추가로 5일 동안 배양하여, 운동 뉴런을 수득하였다. 해리된 운동 뉴런을 배아 줄기 세포 유래 운동 뉴런 배지 (ESMN; 뉴런세포 기본 배지 + 2% 말 혈청 + B27 + 글루타민 + 페니실린/스트렙토마이신 + β-메르캅토에탄올 + 10 ng/ml GDNF, BDNF, CNTF)에 플레이팅하고, 성숙시켜, 안정한 운동 뉴런 세포주를 수립하였다. 안정한 운동 뉴런 세포주의 수립 후, 7일째에 운동 뉴런 개수를 결정하였고, 1일째와 7일째에는 산화 스트레스를 측정하였다.
데이터는, DR6+/- 동물이 야생형 동물과 동일한 수의 운동 뉴런을 생성하나, 야생형 동물과 비교하여 산화 스트레스가 증가되었다는 것을 보여준다 (도 16).
실시예 8: DR6 +/- 및 DR6 +/+ 동물의 표현형 비교
실시예 1에 기재된 바와 같은 DR6 유전자 변형에 대해 이형접합성 또는 동형접합성인 마우스와 대조군 동물의 운동 기능을 실시예 2에 기재된 바와 같은 시험을 사용하여 비교하였다. 신경학적 점수는 도 17a에서 비교되고, 중량 증가 비교는 도 17b에 제시되어 있으며, 로타로드 테스트 비교는 도 17c에 제시되어 있고, 오픈 필드 측정은 도 17d 내지 도 17k에 제공되어 있고, 캣워크 측정은 도 17l 내지 도 17z에 제시되어 있다.
DR6 돌연변이의 유의한 "투여량 의존적" 유형의 효과는, 동물의 중량 증가 (도 17b)에서, 로타로드 테스트 동안(도 17c), 오픈 필드 테스트에서 측정된 양육 및 양육 시간에서 (도 17d 내지 도 17e), 그리고 캣워크 테스트 동안 뒷다리의 보폭 길이 및 유각 속도 (도 17l 내지 도 17n)에서 나타났다.
21주령에 블라인드 주관적 점수화 검정 (도 17a)에서 전체 운동 기능을 평가하였을 때, 이형접합 마우스가 야생형 동물과 신경학적으로 유사한 것으로 나타났으나, 이형접합 마우스의 신경학적 점수에서 시작하여, 동형접합 마우스의 신경학적 점수 쪽으로 향하는 경향이 있었다 (도 17a). 몇 가지 다른 측정이 동일한 패턴을 따랐으나, 이형접합 마우스의 표현형은 약 20주령 후 동형접합 마우스의 표현형 쪽으로 향하는 경향이 있었다 (예를 들어, 도 17f 내지 도 17k, 도 17o 참조).
이 데이터는, 돌연변이에 대해 이형접합성인 마우스에서 DR6 돌연변이가 반가불충분성임을 시사한다. 이 데이터는 또한, 이형접합 DR6+/- 마우스가 나이 듦에 따라, 마우스가, 처음에는 정상인 것으로 나타난 측정에서 동형접합 DR6-/- 마우스와 유사한 운동 및/또는 신경학적 결함을 나타내기 시작할 것임을 시사한다 (예를 들어, 도 17o 내지 도 17p, 도 17r 참조).
실시예 9: DR6+/- 및 DR6+/+ 동물의 감각 지각 결손.
48℃, 52℃ 또는 55℃에서 유지되는 금속 표면에 동물을 배치하여, 20주령에 DR6 유전자 변형에 대해 이형접합성 또는 동형접합성인 마우스와 대조군 동물의 열적 통각수용을 시험하였다 (미국 캘리포니아 우드랜드 힐즈 소재의 IITC). 동물이 열 자극에 대해 재빨리 뒷발을 핥았을 때까지 경과된 시간으로 정의되는 반응 지체 시간을 측정하였다. 마우스가 뒷발 핥기 또는 뒷발 흔들기 두 가지의 평소와 다른 거동 중 어느 하나를 실행할 때까지, 마우스를 플레이트 상에 남겨 두었다.
도 18에 제시된 바와 같이, DR6+/- 및 DR6+/+ 동물 둘 모두는 감각 결손을 나타낸다. 이들 마우스는 20주령에 현저한 운동 기능 장애를 나타내지 않기 때문에, DR6+/-에서 나타난 감각 결손은 정확할 가능성이 있다 (실시예 8 참조). 그러나, DR6-/- 마우스의 반응 지연은 관찰된 운동 증상이 불충분하기 때문일 수 있음에 유의해야 한다.
본 명세서에 개시된 설치류는 인간에서의 ALS 진행을 보다 유사하게 따르는 새로운 동물 모델을 제공한다. 이론에 구애되고자 함이 없이, 몇 가지 가능성 중 하나는, 설치류가 발생 중에 정상적인 가지치기 (pruning)를 나타내는 데 실패할 수 있으며, 이와 같이, 증가된 연결성과 경증의 과다흥분성을 가진 설치류가 발생하고, 시간 경과에 따라 낮은 수준의 흥분독성이 축적될 수 있다는 것이다. 특히, 성체 DR6 녹아웃 동물은 발생 중에 혈액뇌관문 (BBB) 투과성의 증가 및 BBB 마커의 발생의 손상을 나타낼 수 있으나, 본 명세서에 기재된 마우스의 ALS-유사 표현형은, 혈액뇌관문의 결손이 일반적으로 운동 손상이 아닌 인지 손상으로 나타나므로, 뇌 혈관 발생 중에 DR6 역할의 결과일 가능성은 거의 없다.
SEQUENCE LISTING <110> Regeneron Pharmaceuticals, Inc. Ikiz, Burcin LaCroix-Fralish, Michael Croll, Susan D. <120> NON-HUMAN ANIMAL EXHIBITING DIMINISHED UPPER AND LOWER MOTOR NEURON FUNCTION AND SENSORY PERCEPTION <130> T0041US01 <150> 62/133,909 <151> 2015-03-16 <150> 62/250,229 <151> 2015-11-03 <160> 17 <170> PatentIn version 3.5 <210> 1 <211> 58987 <212> DNA <213> Artificial <220> <223> Synthetic <400> 1 ggtgttcctt ggcaactcac tttttctttt taaaaattgt gtgtgtgtgc tgtgtgtgta 60 tgtgtgagtg ggtaagtagg tgtacagcag ccaggggagg atctcagatg tcttccccta 120 tcattctgcc ttgtgccttg tgcctttgag gcagaacatc ttggtgaacc cagagcttat 180 atatgtagac acacacacag atatataaac acattatatg tatataaata tatataatgt 240 gataccataa tatagatata tacacaagac acacacacat atatatgtaa tataatatat 300 gtaatttata tacacatatg tatatgaata tatatatata atatgctacc ataatataga 360 catatactag acacacatat atgtaatata tacaaatatg tatataaata tataatttat 420 gctatcataa tatatatacc ctagacatat atgtataata taacacatat atgtgatata 480 tgtgtgtgtg tgtatataca tatatatata tatatcacgc acacacacat acatacgtct 540 agggtagcaa ccaatcctag taatcctcct gttttcattc ccctcaatgg tctacaggca 600 tgtatgagac cacatatggc ttttatgggt gtatgtgtga tctaaactca gaccttcacg 660 tttgcataat aatcactgtt aactgctgag ccatctctcc ttctctcact ttcctttctt 720 tttgaaaaaa aattttttat acattatgtt tttattttat tttacttatt tttatttttt 780 taatattaga tttttttctt tatttacatt tcaaatgtta tcccctttcc tggtttcccc 840 tccaaaaacc cctatcccat ccccactccc cctgttcacc aacccaccca ctcgcttccc 900 tgtcctggca tgcccctaca ctggggcata tacattaggt tttgagcgaa ttccaggata 960 gccagggcta cacagagaaa ccttgtcttg aattaaaaaa aatttttttt ttaaattaga 1020 gttgctgcca tgagtatgag caacttaaga aaattatctc tcgccgggca gtggtggcac 1080 acgcctttaa tcccaacact tgggaggcag aggcaggtgg atttctgagt tcaaggtcag 1140 cctggtctac agagtgagtt ccaggacagc cagagctaca cagagaaacc ctataaaaaa 1200 ataacacaca cacaaaaaaa atcaaaaaca aaaacaaaca agagagagag agagagagag 1260 agagattctt tccctccaac ttctcccaga ccttccctac ctccattccc accaacttca 1320 tgtttttttt tctctctctc tctttctcaa aaccaaaaaa tgccaggaaa gcaacaaaaa 1380 ataagtagca agaaagtcag aacaaagaaa ttaaatacgt aagtaagtaa ataaaaatag 1440 aaaatcagca atattaaaaa aaaatcaagg actggagaga tggctcagca gttaagagca 1500 ctgattgctc ttccagaggt cctgagttca aatctcagca accacatagt ggctcacaaa 1560 catctgtaat gggatccgat gccctcttct ggtgtgtctg aagagagcta cagtgtactt 1620 acgtttaata aataaatctt taaaacaaga caacacaaaa atccaaaata aaatttcaaa 1680 gtgtaccaac acaaaacatg tagtctgttt tgtgttggtc aactactcct gggtatttag 1740 gcttccttgg agtgtggttg ctatacccag tgacactcag ttaagaaaag tgaatttccc 1800 tttcctggca gggatcaatt gcaagtagct ttttggttag gggtagaata ttatattcca 1860 ccttcctgtg ctggttccac ctgtaaatgt ctgtgcagag ctgtttatgt ttacgtctca 1920 ggcagagctc tgaggaagat attagtaagc aagacattcc ttggagagtg cttgggggat 1980 ccagcaactg tgaaaggaaa aagaaatatt aatcaaaggg ataaattaac cttcaataca 2040 gtttcagcaa aggcctctca tggtgctgtc cccagttgag gcaaggcaac agaccttttt 2100 cctcaccaaa cactcactgg tgtaggggca tggtatgacc tgtcatcagg cagggaacat 2160 tcaccacaca gagtggagag gagctgtggc catctcttca atcagagcag ggtgttggat 2220 ggacgttgcc agcctgtacc acccaccatt cacatcagct gctctgtgca cacagcacac 2280 tccaacccac agagaaggtt tgtttctgtt tttatttttg ctttttatta ttatctcatg 2340 taaaattatt atttatttta tttttgcttt ttttattatt atctcatgta acccaggttg 2400 gctttaaatt tgttatgtag tcaaggagga ctttgaactt cttccctcac ctctagagtg 2460 ctggggtcac aggcacgtat cactattcct gttcacctgg tacttggaat caaacccagg 2520 cttcatgaat aatagaccta aactcttcca aaccaagaag aaccacatct cacgagattt 2580 gacatgctgt gggttttttt ttttcctctc ccccctctgt ctctctcctc ttctgtccct 2640 ctttctccct cttctttatt tctttcattt ttatttttac ttttgaaaag aagctggctt 2700 caaaattctc catatggctg aggacgactt cagcctttaa tttctttttt tgtttgtttg 2760 tttgtttttc taggcagggt ctcactacac agccctggct gtccttgaac tcactatgca 2820 gactaggctg gcctcgaacc tggagatctg cttgcctctg cctcctcagt gctgaaattg 2880 aagacttgca ccaccaaggt atttttagtt aatcgtttga aaaatttata catgtttaca 2940 atgtattttg atcaaatcca acagaaatct tcctctcact tcttctcagt ccccctacat 3000 ctttcctacc ataatttctc tctttctctt ctttttattt ttgaaataac ccaccaagtc 3060 cagatagtac tgtctacaag tgcataaatg tagagccatc ggctaaagca tggccaggct 3120 actagtaatc ccactgaaca ttacacacgt tctttggtca aattaagcaa gcttaatttt 3180 ctgttagaca gggatagctc cctaaatcag actttgataa tatagcatga cacttaagag 3240 aaagtggggc ttgtagagcc tccaaaacct gcaagcctgg gggtttttta aggcttggga 3300 gacttccaga ggaattttga ttgcatagga acttggctag cagaacatct tatcttatca 3360 gggtgcattc aaggtgtgag tcaggctcca gagggtgggg agcaagtcgg attccagaaa 3420 cacacagtga gtgtttggtg aggaaaaagc acagtcaacc tgaattttgc agcagacaga 3480 aatgaacttc tgaccttata acaaaatggc ttccgtcctt aagatggcgt caggctggtc 3540 catcagtaac tacacccctg aagaaaagtt atttcctctc cctaactgtc accaattgcc 3600 agcaacttct cagctaggaa tagagcctca tgatttcctc tcccactcat actgggtgtt 3660 gactggcttg agtgccatgc cacgtcctga agacagcatt tcacagcatt tgtccctatc 3720 ctctggctcc tacggttctt ttgttccctc tgctgagatg taggtacccc gagccttgta 3780 gtagaagtga caggcatgtc ccatttatgg atgactactc aatggtcaca tactctcaga 3840 actgtggcca gttatgagtc tgtgttgact gctgcctact gcaaaaggag tcttctctgg 3900 gcaaaacagt agtaatctac atgtataaaa cacaaatgct tacaaggtag tttgatgggg 3960 gggggggact gtcaaacaac ccttgcacag gggtcaccta ataccatctg cacaggggat 4020 atttgtgcct tccctcagcc ttgagtaggc taaacaaacc catgatagtt ttacaaccta 4080 gctggagtct tctgacctca ccacatttgc aatttcctat agagctttga ggagtagact 4140 gcccactgag cttgctttgc aacataatct ggctgaaacg aaggatgggg tttgtgggct 4200 ttctcagtgc tgaaaattaa ctttgagcct tgatcagaaa cgtttgtctt tgcttcatcc 4260 ttctcttgca cccagttttt ttctcagttc caggcccccc cccgccccgc ccctttcagg 4320 tatacccagt ttgtccatgg ccactggaca gctacagata tttacattac gattcacaac 4380 agtagcaaaa ttagttatgg agtagcattg aaatagtttt atggttgggc atcaccataa 4440 cacgaggaac tgtcttaaag ggtcacagct ttaggaaggc tgagaaccac tgtcctgcag 4500 cctagctctc atgggtcctt gataaggttt gtagcaccag acatgaactt tttcctgtgg 4560 agcagccctc aaagacagtc agaaagagca gttggttatc ccaacaacca acatgtcact 4620 attatatcaa gggggctttc ctatagcgtg aatggtcctc agagggtaag tccatctttt 4680 ctccccacgt cgcagggata gcatttcctg gcaccatgaa agcaggccag tagaaaggaa 4740 gtttctaagg accttgtact tctgatcctc ctcctcgcat gtgttgggat tacaggcctg 4800 caccaccaca tccagtttat taggggctag gcatgtgcac agtaagccaa cactcaataa 4860 gcagaacgac actcagacct cagccccttc atttagtgtt agtgtgttgg tggttgttta 4920 ttattattat tattattatt aattcaccac acagcaattt atgtcctaca tgtcacattc 4980 atttttaaat tccctttact ttttccccta ctttgtttta gaatatatgt atatgtgtat 5040 gtgtatacac gcacacacac acatacatat atatgtgtat acacacatac atatatgtgt 5100 attcatgcat atacatatgt gaatatacac acatacacac gtatatatga gtatacacac 5160 atatacatat ataatatgtg cgcacacata tattttatat attagcattc cagcttaggg 5220 gcttcagtgt agctcattgg cacagctctt gtctagcgtt ccagttttat ttctactttt 5280 ggcttttaag tctatttgtt ctattgattt aaagcttaca gtacctcggt gggggcacac 5340 acctttaatc ctagcactct ggaggcaaag gcaggtggat ctccgagttt gaggccatcc 5400 tggtctacag atcgagttcc aggataaacc gaaagtagag aaaccatgtc tcagaaacca 5460 aaagctaaaa caaacagaaa agattcacaa caaatatttt taaatcatta caatatatta 5520 caatatcatt aatattacac ccctttaagg gtagtataag aatctttatg ttatgtgtct 5580 ataaaatggg ttgaggagct ggtgagatgg ttcagtggat gaaggagctt gttagcaagc 5640 ctgccaactt gagttcaatc cctaggaccc acgtgataaa aggagggagt agattctttg 5700 aagttgtcct ctgacctcca catgtatact atgtcacatg tgttcggcat aaaataaaca 5760 aaaacataga agggagccta ggagacagtg ccgttggtac aaagcctgct gcaagagcat 5820 gaagccctga gtttgagccc tagcactcgg tcttatgcat acagcacatg tctgtaatct 5880 cagcactggg gagacaaaag agtgagtggc cctggagttt gctgggctgc cagtcaagcc 5940 aaatcgacta gctccagata gggagtgggg cagagaaaga ctgagcccat ctcaatggtg 6000 ggcaatgatt gataaagaca tccagtcttg ccttctggct tcccataagt gcatccctca 6060 gtgatatact gttacctgga agagtaagcc aaccaagccc tttcctccct aactgatttt 6120 ggttggaaag gaggccattt ccatgaagct aggaattgac cttggatgga agatgctggc 6180 ggaagtttct ggtttagtcc aggtggcccc cgaagtgtgg gctacaccat ttcccagaaa 6240 cctctagtag gcatgcactc cagctaccca tgctggacac tcgttctttg atctctctcg 6300 tttactttct tattttcctt atattatttg ctcgtttatc cccagacatt cctgggactg 6360 tcttttacac aaattcttca catcccatga tgtctttccg gctctccgtc ttggaagaaa 6420 acacccaagt gactgactgt gaccaaacgg ttacatgcaa atgtatgaaa tctggggacg 6480 aatgcagtaa taaaaggtga cttaaagggg ctggagtgat ggttcagtgg ataagagcac 6540 tgactgcact tccagaggtc ctgagttcaa ttcccagcaa ccacatggtt cacaaccatc 6600 tataatggga cccgatgccc tcttgtggtg tttctgagga tagcaacagt gtactcatat 6660 aaataaatca atcttaaaag aaaataaaaa acatttcatt aaactcgata ataggtataa 6720 atgcgaactt aatggatgtt ctcagggaag ttgactgtaa cttgtaattt gttcagtgaa 6780 tagcaagcat ataacaacca gacggctaaa actaccacac cgctaccaca catgtttctt 6840 tacgtatcac atctgcattt ccccttgcct caagcatagt acttggaact ttgttcctca 6900 tattttatga ctgccttcat ttttttagaa aagagttgtt actattatta tatgtaagta 6960 cactgtagct gtctttagac acaccagaag agggcatcat gtggttgctg ggatttgaac 7020 tcaggatctc cagaagagca gtcagtgctc ttaactgctg agccattttt ctagctcaag 7080 tgcctttgtt ctttttctgg agtttctgga tctagacagt gtgccttaat tttgttcttc 7140 agtgatgttt ccagcctgca accgagcttc ccttcctcgg ctggaaaccc atctttgcct 7200 tctagattcc tgaagaggtt gaatttcatg tgctgactac aggtcccttc ttcaaaaccc 7260 attctgccct cagaatctat gaacccgatt taaaaaaaaa aaggattttt atttttatgg 7320 gtttggctat tttgcctgta ttcatatctg tgcaccacat gcatgaagtg cttgcagagg 7380 ccaaaagagg gcgccagata tcccggaact ggagttacag atggttgtgg gccagtgctc 7440 tggaaaaaca gtcagtgctt ttaaccccta agctatctca ccagccctca tcttctctta 7500 gaccgagtgc tagggctcaa actcagggct tcatgctctt gcagcaggct ttgtaccaac 7560 ggcactgtct cctaggctcc cttctatgtt tttgtttatt ttatgccgaa cacatgtgac 7620 atagtataca tgtggaggtc agaggtcaac ttcaaagaat ctactccctc cttttatcac 7680 gtgggtccta gggattgaga agtcatctgt gatttttgac ccagctgccc tacctcattt 7740 tgcagggaac agggatgggg atgggaatgg caaagggcag gacactgttc caccttccgc 7800 ctcaaggctg ttaacaaggt ggtttaatac tgaattgcca gcccagccca gcccagcctt 7860 tcactatttt cccggtattg tcaaacattt cctccacact acctatagaa tattcttact 7920 tatgtttaat gatctacttt agtgtttatt ttaagtacat actatgttcc gttccttgac 7980 ctcgctgtac tttgtttatt cgtaaatata cttacccacc tgttgtaata aaaatgtcct 8040 tctgtccttc cttcctttct tcctttcttc ctttcttcct tccttccttc cttccttcct 8100 tccttccttc cttccttcct tccttctttt ctcccttcct tctttcctcc cctccttcct 8160 tctttcctcc cttctttctt tcctcccttc cctccctcat ttctttaacg ttcaaccaat 8220 gtttctttct cacgtttcct ttccaaggtc agttccgagg gctggacaac cacactaagc 8280 tcagcggctc cgcacatcct gccctcgtgg agcccatgtt aaagagaggt tggtggagac 8340 acgcaatgga cactggagac aacgacttag cttagtaccc taaaagtgaa tggaggatat 8400 gcaaaaataa gatagagggg agagcaaagg aatttggcgt ccagcgttag tgtgtgtgta 8460 ggagtgcgta gtaaagagca gaagctcgtt acgtgtgcag cggatgtgag agtctcacag 8520 agggaacccc cagcgctaag ttcctgaggc ttggtgggtc aaagaacaaa ttaacctcag 8580 catagttacc cttccataag caacaaattc tccaggtcca ctaagcctcg tgatcagcag 8640 cctgtacagt cacttatgag gaacttttat agggagaaca ggatgtgatg gtgtatttaa 8700 acatatgtat ttaaagatgg ctcagtgaat aagggtgctt gttgcccaga cctgagttca 8760 atccttgggc tccacgtggg ggaaggagag aaccaactcc tgatcctcca catgaatgct 8820 gacatgagca tgcacataag caatacatga gtctaatttt aaaaaaggaa aaatacctct 8880 ggttaacata taataaattg ctattctttt agatggttgg attcctaata cacaccagca 8940 cagagtgaac gcactgggta atgtacaata gcaaggtgac ttacatcaaa ctacattaaa 9000 tgtttaaaac agtgaaaacc tgaaaagcta acatttttgt ccaatgtgaa aaaaaagtct 9060 tatttagtag catgatatac acagaggtaa aaatagatat cagaaagtcc cccacatatg 9120 ctaatcaatt ttgttcttaa tctttacata tccacttatt tttgtgatat ctttatttta 9180 tgcataaggg tgttttgcct gaatgtatgc ctttgtaaat acttgcatgc agtacccaca 9240 aaggccagaa gagggcgtga gatcccctgg aattgaagct actgatggtt gtgaaccacc 9300 acgttggtgt tggaaatctg acccaaatgc tctgcaagag tcgccagtgc tctgaaccat 9360 gtccaagtta tctctccagc attatatatc cacgtttgaa gtagaggtac attgggcttt 9420 tcatttttat agtggttact taaagcatag tctatgctgt ttttaagtga cagaatgttg 9480 ttcattgtat ttaaaaaata tagacaaaaa tttaaacact ttaaaaaata ttatttttct 9540 ttatgaagat acatgctttt aaggggttct gttggagaat ttcagtagcg ccaacttaaa 9600 acaaatactt tattagggct gtagaaatga cttagaggtt aatagtgtgc actgctcttg 9660 cagaggatcc gagtttggtc cctagcaccc atgtcagggg cttacaacac aacctcctgt 9720 aactgatgct gcaggggatc ccaccccctc ttctggcccc tctcatgcac aacacacatg 9780 aggcaggcac agactgatgc acatatatgt gaattaaacc ccaaataaaa tcgttttcaa 9840 aagtccaatg aatgtcttga caatgctttt ataatcaatt cattcatgac aggtggttcc 9900 ctgtgtttgt gatttctctt caagctgcgc atcaaagcaa cccctccttc tccctccttc 9960 cctccctcct cctcctcctc ttcctcgttc tcctccctct ctctgtgcat gtgtgcctgc 10020 ttacctgttt gcgcaccgca ggcgtgcagg tgcccacgga gtgattaatg aattctttgg 10080 aactgggtta caggcagttg tgggcgatgt agtgtaaatg ctgggaacca aactcgggtc 10140 ccttggaaga gcagcagtgc tcttcacaga tgagccatcc ctcaagtccc caagaacttg 10200 tttctcttgc tcagcttgca ctctccaaga cttaggaaag tgcttagtga ataagctggg 10260 gcacaaaaga tgcggttata agaaggtgac gtaggagcct ctggcaagcg atgcagatgc 10320 caattctcac ttgtgttccg gtccctctcg cttttgtcag ttttagaatg ttctaggctg 10380 ggtttggtgc tgtcgtagca cagagaggaa acagcatgaa ggacaaccag gccagagata 10440 gttgatggtc tgagcctttt gtttgctggc ttcactcgct ccgctgttct aaagcttcag 10500 tcaatgggtc tatgttgaac cagtttccgc tgttcactac aaagatgcaa ttggaagccg 10560 agggagggag ggagaagctc tggagagtat ggctcagtgg ttaagagcac tgactgctct 10620 tccaaaggtc ctgagttcaa atcccagcag ccacatggtg gctcacaacc atctgtcatg 10680 ggatctgact ccctctgaag acagctacag tgtacttaga tataataata aataaatctt 10740 agaaaacaag tagctgaggg aggagttttt tacttgtctc atccagggtg cctggaggat 10800 gacggcgagg cgccactttg cttacctcac caagtgagag ggacaaaaca tagttttgat 10860 ctgcagtagc ttcgcggtga gcaccacatc tccacctagt cctccgctat tgtcggctgc 10920 tatgaacaga ccctgctgtc acctggccat ttatctccat ggtcctgttt aagctctagc 10980 ctattgacta aggggatgca agttgaagtg gatgtcccca cgtttgaaca cagcactaca 11040 tgctggtatc tgaaatctga gtcacatcta ggtcagtcac attttctaga tttatgagtc 11100 tagattttat ttttccaggt gtcttgtttt tctgtctcta aatgatgaca tccacccccc 11160 tgccttcccc ccccccccca cacaccatat accctgggta tggttatttg tagcaaaatc 11220 aatactcttg tacttgaata atttttttct ttgcattttg aaccaaggtg tttattcaca 11280 aaaggaagaa ttgtccattt gtctgtctgc tcagtctttg gcttagcata gggcatgtgc 11340 agaggctcaa taaaaatctt ttgcctcaat cagtacatgg cacatgcatt acctcagggt 11400 catatttgta aataggtatg ggtgtttgcc tacaggtgtc tgtgcaccac ttgcatgcag 11460 gtgcctgagg aggccagtag agggcatcag atctctggga actggagtta cagatggctg 11520 tgagtcacca tatgggtatt gagtacaaga gagactctgg aagagcagac agtgctctta 11580 gccactgagt catcactgta gcccgcatgc catattatta aggaggctga acaccaagtg 11640 attcagcctc gctacagaca agtagaaacc tgtctctggc ctccttaacc acaaagttgg 11700 ggggcttccc tcgacacaga agccagagaa ccatttgaca ccgttcctct gtcacaggct 11760 gtatcattgg tccattttcc acatgacact gtgcattcaa gcattcatct ctgcagatcc 11820 ctgggcagta tggcagtgtg tgtgtgtgtg tgtgtttgtg tgtgtgtgtg tgcttgtgtg 11880 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtttgtg tttgtgtgtg tgttctaatt 11940 ggaaaggaaa tcttcacttg cctttaaagt gcagtgctac tatatttctt tgcaaatata 12000 tatacgtata tgcatatgta tatgcagatg tatacacaca cacacacaca cacacacaca 12060 cacacacata tctcaaagtt cctttcaggc tgaagaatcc attattgtcg ggccagtgag 12120 atgacagagc agtttaaggt atctgctgcc aagtctgatg acctgagctc aatccctgaa 12180 atccacaccg tggaagggga aaactgtctc ccacgagttg ttttcttgac cgccacaggt 12240 gtatgcaaat atgcatgtgc acgtgcacac aagcactaaa taaacagaaa gtttaaaaaa 12300 ttaaggtata tattatttac tatgtaagag aacatcaaaa aagtcttgag aagtacacca 12360 tgggagaaaa cacacctagc ccttcaaata atttgccctt ctagaaaaat cctgaaatag 12420 ccctttcccc atacagtagt agaatgataa cattatctca gagatgagca ggcgtttaag 12480 gctcagcgga acgggaagag agttgggttt ttcttcctat ttgttttgaa tagctctgct 12540 cccgggtcct ctgcttgccc agatgatctg tgaaatgggt gagtgagcag cgcacctccc 12600 cagcgcgact gttactgacg tcagcaagcc tcattagctg ctgtttgtct aaccaaaggg 12660 aacagcgggt ggagagaatc ttaatgacct gacttcccac actgcaggtg ccacttgcct 12720 ggtgtgcacg ctgagattgc atcagctgac taggcaaaca aggaacagtg gaaatgggag 12780 gcagagaaaa agcccaagag agactgccca gtgagtggaa ctcgctgctc catcctggga 12840 gagtgtagca gagatgtttg tgcgtggtgg agtgaggtcg actcaggttc acagctgtgc 12900 gctgctacat agtagttgag ggtcgaatca cttaaaggct ttagtcccgt tttcaaaatt 12960 aaaataggga taatgataga accttaggga gaaggaggga cacgtgtgta aatcacagac 13020 ctcattgtac cactggataa ctggcaggag ccactgtttc tgactctaca gtggagagaa 13080 gggaagagca atgattggca ctgtggacgc aggtggcaga gaacagctaa agatgacttt 13140 atatcctaaa ggcaagtggg gtagctgtga gttttaaaga ccgaaccttg cttcacaaaa 13200 ttgctcttgc tttcctacct accaattctg cgttctttct ttttccctgg cagcatctcc 13260 ccggcggact cagccttggc tacagcggag actcctggga aggaaatgtc cttccccagc 13320 cctaggcaca gtggttattg tctgttctca catcctgtga gccctgtagg cagaggaatg 13380 ccccagaacc acagcaactg tgcaaacatt tgaattccca cgggtagttt cccatcctcg 13440 tacccatgag gcttctgctc tctgtagtca gaagaacgta cacagttctc aggagcagga 13500 agtagagggc taggaatcat gggtgaggag cctagcatgt gtgtgaatac atccgtggaa 13560 aacgtttttg agggagatag tacagaatcc tactccaggt gaaataatga gactcttgct 13620 aagaccacgt acggatgacc caatcaatac acaaggaggg agttgaaaag gagaaaaggg 13680 gttggccatt aatttagcca acgtataatt ccacgtatta gcaagtaact ttaattatcc 13740 tatgcgccct cggttttatt ctccacagct gtaaatggta ataatacccc ctttgaacat 13800 ttatcatgat taattaaggc agtgttgttg aaacatattg aaagttacct aggctaactt 13860 tgtccaattt gccagctgct ttggcaaatc ctctgcactg gggctggctc aggctagaac 13920 tatcttccat ctgggtaaat ctcttctcca ttccagagca gatatcatgg cgggtagctt 13980 gacctcacag tcatgagaca actcaaacca tggggattgg caaatgaagc aagtccttgt 14040 tattgaactc tccactcaga aaaaataaaa aataaaaaca tattaatact acggaggatt 14100 ccagtgacac tcccttctgg gaaagacact caagagattt ttatgagcag tcagggcaga 14160 tggaagtcac aaggcaaaca cctcaaagta aaaaaaaaaa aaaaaaagat caacatacca 14220 gagcactcaa caagctgaca gtctggctgc ccatgctaga tgcttagaat acatgaatga 14280 caaagtttgt atgcagtctc tgcctttagg gactttactg ctaatggggg attgtttatc 14340 aaacctaaat aatatccttg agggaatatt actatactgt gggtattagt gttcttaacc 14400 aatatgggtg ttgactttgt ctgtgtctct ctctgtgtgt gtacaacgtt tgggagtcaa 14460 ttctgtcttt ccaccacatg ggtcctggct atcaaactca gtctctcagc attactggaa 14520 gatgcctttg ccccatgagt catcttgaca gcaaaattgg gtattgagaa cttgaaagtt 14580 tgctgtgtga agaataagta aaacatgcta aaaaaaaaat aaagtagaca ggctgcacag 14640 atggctcagc agttaagagc actggctgtt cttccagagg acccaggttc atttcccagc 14700 acctacatgg cagctcacaa ctgtctgcaa cttctgttcc aggggatcca actccttcac 14760 acatgcaggc aaaaacacca atgtacatac aataaaaata aataagttat ataaaaatag 14820 gcaatctcat agttttacca tgttaattat atgttaaact agcaatgtgc atatattgca 14880 ttaaataaaa atgattcctt aactaaatta atttctttaa ttaaataaaa tgcaaaaaaa 14940 tgaatgaaat aaaatcactt tcacaaggtg gagaaacact ttgggaagac atccagatgt 15000 gaacctcagg cctccacata cacaaacatt aataagtatg ttaattaaaa ttaatttcac 15060 tttactttta aaaatagaac tactagattg tttaggatag aatatataac ttctgcccaa 15120 tatttgctca tattcttttg gaaaatatgt gttcaaatcc tttgcttatt cttaaattag 15180 attgcttgct tttattgttg tatttttttc tatcccaaat agccaaatag tatatttgtt 15240 tggaggtgta tgtggctgtt ttaagacagg atttcttttt ctttttcttt tttttttttt 15300 tagctctggt tatcctggaa ctcactctat agggcagatt ggcctcaaat tcacagagat 15360 ctgcttgctt ctgcctcctg catgctaggg ttaaaggatt gcactggtgc tgggcatgac 15420 aacccccccc cccctttttt tttaaccttc actggtatag tacaaatatg gcacacagac 15480 cattcaccca tacacataag agaaggacta aacaaatatg tttaaacatt aaaaaaaaaa 15540 aaactattgg aaacggaatc gtcaaactgg aatggtggag atttcatcag aactggcttt 15600 gttcattttc ctgtctccgt caaactgtct gctctgtcac agtctggttc tccaccacgt 15660 ggctgctgct tctgttggtt cagatccctg ctccgcagtc tgagccaatg aaatcccggc 15720 tcttcacaaa ggcggtttta gtggcaagtc tttggggctt cttctgttct cttggaagaa 15780 tggccccagc agcttcttcc atagcgcttc tggtgaacca gatggcatct tgttcaacct 15840 cttactgcac tgagcacgca gcctcccctt acaagattca taccaatgtc tcattgtttt 15900 ccaaagtaac ttgagacttg aactttccca gactctcaaa taaaaccatt ccttgtggaa 15960 gagtgcggtg gtctccagtc tttagagact gcctgcctac ctaagcttta ggggacagag 16020 tgggacaaca ctaacaaggt gtgtgggcat gacaacctct aatcttctca gctagcttca 16080 cctggaatct cagtcttatg agtaatctgg actaacagta agggactcat tatttgatgc 16140 ttgccacatc tggggtcaaa tcttggcccc tagaaatggg agatggatgg acaaagatgt 16200 cctatacctt tagctatacc ttctaagaat tcagcctcaa cagcttggag acagacggga 16260 ggacaaacag ggtgggcttc cctggttgct gggcccgctt agggcttggc tgtaggctga 16320 aagggaaggg agcttgctct ctcggccaca cactgtactt gcatctccat cgctgggatg 16380 cagacagtga agagaacaga ccgtagctct gcttgttctg agacatagga aagcgctcca 16440 acaaatgttt cttcacctat cgtatgctct gaggacaatt tccacagact ctagatgatc 16500 atcgttgcac aatttttagg aatcttcgca gtttccttga agtgtgtcct caagagttcc 16560 tgatgctgcc gttatagaag tgggagtagt tgggggtttt atagacaaac tctaaaacta 16620 aaagaaggag atatacacag ttacagaagt gtttggtcag caaggagtca aaaaattagc 16680 cagtgcacac taggcagcat attaaagtat ataatatagt atatattata tactatatat 16740 attataaagt aatgtattac aaagtaatat attactatat attataaagt aatatactat 16800 aattaatata taagtaatat actataacac atataatata taacaaatat aacatataac 16860 atgatatatt atattatatt atagtatagt atagtatagt atatataata agatactact 16920 ggctactaat ccatccaaat ctagaatgaa ccctgtgtaa acacacagga aaacagtggg 16980 aaatctagta gaaaactgga caataggtat taataggaaa ttcacagaag gcaaaacttt 17040 tcaaaatgac aagttcatga atggggcttt atcactatta aatcctttaa aatgcattta 17100 aatgccaagg cgatggctca gctgttaaag agcttgtggg gggatcagga ggaccagaga 17160 ttggatccta gtatctgggc caatgtcaga aggatgtggc agcctgccta taatgctagc 17220 tttggaaggt ggagtcaggg tatccccaga acaagctggc tcgtgggatt agccacattg 17280 gtaagctctg ggtttaattg atagagcctg aatcaatgaa gggaggagag agtgacacaa 17340 agagactcct gatgtcaacc tcaggactct cttcatgtat gtggacacat gtgcatgcac 17400 ccacacacac gtgtgtccac atatttgaaa acagatattt acatacaata cacacacaca 17460 cacacacaca cacacacaca cacacacatt caaagtaagt aagtgcataa aaatggttac 17520 attgtagcag tttgtatatg cttggcccaa gggaagtggc actattagga ggtgtggcct 17580 tgttggagga agtgcatcac tgtgggggtg ggctttgagg ttcttttttc cattttctct 17640 atttgatttg attactacag ccatgaaatt atgatgatct acatatacag attttatgac 17700 aagaaaagaa aaattcataa aaattttgaa gctggagcag tgattctgtt ggtaagatgc 17760 ttgtaagctc tgggctcata tccacttatc agtgagtgca tatcatgtgt gtccttttgt 17820 gattgggtta cctcactcag gatgatgcct tccaggtcca tccatttgcc taagaaattt 17880 cataaattca ttatttttaa tggctgagta gtactccatt gtgtaaatgt accatatttt 17940 ctgtatccat tcctctgttg aaggctttga ggttctatgt ttaagctcta ctaagtgcag 18000 aagaaagctc tcctggttac ctttggatca aaatgtagac ctctcagctc cttctctagt 18060 accatgtctg cctgcatgcc gccatgcttc ctgccatgat gataatggac tgaacctctg 18120 aacctgtaag ccagtctcaa tgaaatgttg tccttcataa gagttgcctt ggtcatggag 18180 tctcttcaca gcagtaaaac ccaacaaata tcactaagca atttttaaaa ataaagctaa 18240 gggctagtga gacggcttgg cggttaggaa tgcccgctgc caagcctgaa gacttgatga 18300 gtttagtttc cagaatttat atggtggaag gagagaacaa actcttagaa ctgtcttctg 18360 accttaacac acactgtgtg ctacactcac gcctacacaa atgcatgccc acgtaaacaa 18420 atagatgcga aagtcaatgt aaatacaatt ttttaaaaaa aaatgaataa catgcagtta 18480 agatgaggtc atattatact ctcaccattg gacaaaactt atatgttatt gtcctcattt 18540 ggttctgttg ctgggataaa atgcagacca gaactaacct gtggatgaag gggttcattc 18600 tgtttatagt ttatcctcct tcactgaggg aaggaaaggt ctacatcacc cagaggaaga 18660 actgaatgaa gcaaagacca tggaggaatg atgcttactg gcttgccccc aggctcaggg 18720 tcaacgacct ttctcatgcc tcccatagcc acctgcccag ggatggccca gctcacaatg 18780 gcctgggtcc tgtcacatca atcaacaatc aagaaccttg cctcacagac atgcccacag 18840 gccatccctc aaccaagatc tctccctccc acatgactac agtttgtgtg aagttggtga 18900 aaaccagcta gcagtttatc aaatcttggt gggaaaaaga tagagagaca gaactacgct 18960 ttgaggagca acttggtggt actaagtgaa attaagtata tataattatg gctaaggaaa 19020 taacattctt gggttaatac cttagaacat cttccataaa caaactagag gaaggtaatt 19080 taaagcacaa ataggcaact aataccatct taaactaacc tacacaccta cgggttatga 19140 atggtgacca caggctctgt atgtctacta agaaataact atgtgagtca aaatgaaaga 19200 accaatcgat ctacatacgc cagcaaggac ggtttctaac tgtgcttcgg atcgtaaaga 19260 taacaaagca aaattagatg atagcatatt aaaggttatg taacatgtga atataatggt 19320 ttctgtggtt cattgttaca tccgtttatg aatgaagctg tggttggtga atggtatgca 19380 ttaggataat aagcgtgggt acctggtaag agaagagatg aaaatggctg gaggaggcag 19440 tgattacagt tccattcaga agggaatcta gagcctggga gatggcttgg taaagggctt 19500 gccaagcaag catgaggatc gaagttcaaa tccccagtcc ccgtgtaaaa agctaggcag 19560 agcaacacat gctcagagcc gaggagtcag agacaggcag atctctggag tgcactagtc 19620 agctgactca gctgagtccc tgcactccag gtttagggag acactgtctc aaacaaacaa 19680 acaaacaaac aaacaaaaca gctggaatgt atgctgactt agttttactt gacatagcta 19740 gagtcacggg agaagaggaa acccccaatt gtgaaaatgc ccccactaga ctgacccatg 19800 ggaaagcctg tagtgaattt tcttagtgac tgctgtgaga aggcccagcc cagcatgctg 19860 tgagtgggtt gtcctgggtg ctgtaagaaa gcagactgaa caagcatcgg gaggcaaaag 19920 ctgataagtt gtcccttcct gtctccaggt tccttccctg atgtccttca ctgatggagt 19980 gtatctgaga gttgtaggct gaagtcaacc ctttcctccc caagtagaca ggaggttaga 20040 aagatagccc tgtgggtcag agtgggtgcg gtacaaatag gaggatgcac cttcatatct 20100 caacctttgc atgagcatct gggtgtagac acacttatgc ctgtgacacc tccttggagg 20160 tggtgatcgt gggactgact ggccaccagc ttagtttcag gttcaataag ctaacctgtc 20220 tcagggaagt aagatgagga gtgagagagt ctggaacacc ttctctggca tttgcacgca 20280 catatggata cctctccaca tgcttataca gtacatacat acactccact ggcatgcaca 20340 cgtacacatg cacaccaaca acaaaaaagt aagttgaaaa aaattaaaga aacactccat 20400 gttaacttat ggcctttaca tgcatacata tatatgtgca cacgcacctg catacactta 20460 cacagaggga gagattacat aggcgttgtc agcagtgtgc tgcagacaaa gaggtatgat 20520 cagtctttct gagatgaatc ttagtgatga ggattccatg atgtatgagc aacagggctg 20580 ggcagtgcag ccagctggtg ctgcatctcc tttcagtttg gattatctta ttgggtcaca 20640 ccagaaccag ggagataaat agtcaagaca gactttgaaa attcatacac cacacacaca 20700 cacacacaca cacacacaca cacacacaca cacacaccaa taaagctagg tttgaatgaa 20760 ttacttttgt aaaatccagg tctgagttgt attctgaaag ctttacttgt ttggactggc 20820 cagtagctca gtcaggcaaa aagacaccac acgggagggc agactttaaa gaagaacgtt 20880 tcaatgaggc aagctgtcac ggcttgtgtg aattacacaa tgaaagggaa aataaaataa 20940 aactatggcg ggctaaaatc tgtaagttct gacatgtggt cctcgtggca ccatctgctc 21000 atgaaagcat gaagaggctt ctggaacttt ccaggactgg gttgatttgt ctccacaaag 21060 ctgcctgggt aagaccctgt ggcactgggt catcaagaag gtactagcag acttcctttt 21120 tatctcagta tttttctacg tcagtcccca aaccatgaaa caacagggct taccctgcca 21180 caccaaaggt caaactatgt tttctcttac ccaactattg cctgggatgg ctccaacttt 21240 attactaccc cacctccaac ccgtaataac tctccagctc tcacttcaaa tccaagagag 21300 tgtggactga gtgaggggac cagagacagg tgcatctggt agaaagttat actcaattaa 21360 ctaactgaat gcatttgcat gtggttttaa ttccactgtg cagcaatttc ctgagtaaaa 21420 atggagagat tatatctagt ttttgagtca ctgtaagaat taagtaaacg tagctctcaa 21480 tctatatctg cacattattt ttcctcgtgg tcctagattt ctcttgttcc tgaccatttg 21540 tcccgtcttg gaatttcccc agccttcaga ggtgtagccc tataatttac cgtccagatt 21600 gcaatccctc ttcatggcat gaattaagtc ttatctcacc aggaagtgct ctttcctttc 21660 ttctgactcc tctccctctg gctttcctct tcatttcttc tctacactgt ttagggaaag 21720 aggtttctta gcaaatcctc agacacccca actagtgggc ttcccggaaa gtctcagccg 21780 tgtgtgggag gtctgccctg aagaagttct ttgccagaaa atgaagcaag gtgttctggc 21840 tggacagtga gggtggtaga ctgtaataag tatgtctggc tttaactctt ctatattcat 21900 ctgaggggag catctgagcc caacacaaca tctccatcgt caatggagaa tcctactcag 21960 ctgacaaggc ccttgcttct gtgttgaagg attacagcaa tggcctcctg tttattgacc 22020 tcatacttag acccatgagg catgcatatc atagtttatt tctcctactt agcaactgaa 22080 aatactaaag ggagtcacag agtctgactg ctctcacggt ctgtatctat tgcttaatcg 22140 aaagcttccc tgttaccctc ccatagcaaa taagggtcca tcccacaaga cactgtggaa 22200 acgcctcctc tcaagctcct ccagacctcg cagtgagtca atagagagca gaggggtctt 22260 acgtctctgg tgcctaaaga ggatggggtg ggcgttaggt ctcagcaaga acatctcttc 22320 ttatgtggcc agcggagagc ccagaaatct taagcttttt cataagggtt tctttgagac 22380 gcttcctaac ctagtgatcc ggttatcacc aagctaaggg aaaacgatca cgtgacacga 22440 aggatgaagg aaaaccctca gattcctcct cctctgaatc tgggagtgga aaactgagga 22500 gctgagctca gagttccaga gccacgcgaa gctgggctca gcagtgtttg cagctgcagg 22560 tctggtgagc ctgtggaaag agagaagggt gagagaagag agtctccaaa aagctgcccg 22620 aattagagaa acaagagacc ctgcctcaaa aaggcagaca cctcaagtgg tcctctgact 22680 tctgttcatg tgtcatgaca cgagcatccc tgaatccaca tacacagctg agaagcgtgt 22740 acacacacac acacacacac acacacacac acacacacag agagagagag agagagagag 22800 agagagacag agacagagac agagagacag agagacagac aaagagacaa agagacaaag 22860 agagagaaac agagagtggc tgaagggagg gggcagggga aggagggagg gggaggggga 22920 gagacaaata atttataaaa ggaaaatcat ttaaaacatt tcagggctgc cgagatgacg 22980 cagttgcaag caggaagccc tgagtttgga gccccagaac gcatccaaaa gccaggtgtg 23040 gagcgtgtgt gttctgtatc tcagctctgg aaacaagggc ctcgccatct attgccaatg 23100 ggctgccaaa ccaacaagga gcgagcaagc cccacttttc acgtaaaata cctcaaagag 23160 aaaagcaaca gatgcaaagg ggagagaatc tcgcttttgc ccagatcatg tagcagcccc 23220 gctcttagag gacgtactcc atgtctatgt gacttcaact ttcaattcta caaaaagggt 23280 tggtttcatc cctctcttct caatctggaa acagttcgtt ttaacgacag atgggtgata 23340 ttacacttag aatcaaactg ttaagccatg tgaatgaatt tatggtcacg tcatttcgaa 23400 gcgtcccctc tacaaccccc ctccccccgc tgggaaaaga ggggcttccc gctttcccag 23460 gaagggaagc tgtgggctct gctgggggac acacactgag ggactttcat cagcatccct 23520 cccaagaggg aagggacatg tctgtgggcc ttatggtctt caaagggacg taaagccgtt 23580 gcattctgat tagtttgtgg agggtacttg tttcaatcca gctacagttt ggtttgtgca 23640 gctggtatct gctgagcagc tgccgggacc tgtgtactct cgaaatcggt gaggctaacc 23700 cctgagaagc agagggtcgg gtgtcacgag agacttttct ttcccttttt ggcagccata 23760 ttgaaagatt cacagacacg ctcttaactt atccatgcaa agtgagactc tgctcacatt 23820 ccctgatact ttccttcaaa atgcaccagg aaatgtcagg aaataattca attagtctca 23880 ggaaatttac aaactgtgca aaacgttttg aatccacttt tagaacactt tcaatgacca 23940 caaaagctga agaaagggca gaaagaggca agggaaagag gaaaggaaga aaggaaacaa 24000 acaagcaaac aaatccagtt tgtcttcaga aggattatgt tggaggttcg ttttgcttgt 24060 agagactagt tcatctattc caggcttgct ttgaattccc tgtataatgg agacaggccc 24120 tcaacttctg gtccttcggc ttctgtgtgg cattacagtt gagtgctatc acacttaatt 24180 atgtcagagg cgtttttgtt tgtttgtttg tgtttatgtt ttactttagc agtggctgga 24240 gttggggtgg ggttgggtta ctccagcagc tcccaagagg atcagccttt caggctgtcg 24300 ctggaagggc agctatatag atgctgcaag aaaagcaact agctcctgct cttagagaac 24360 ttttgtttat tattattatt aatttacttt atttacattt aaaatgttag cccctttcct 24420 ggtttcccct tcgaagaccc cctatgccct ctcccctccc cctgatcacc aacccaccca 24480 cacctgcttc ctggccctgg cattccccta cactggggcg ctgagccttc actggaacaa 24540 gggcctctcc tcccattgat gactgactag accatcctct gctacatatg cagctggagc 24600 catgagtccc accatgtgta ctctttggtt ggtgatttag tccctgtctg ggggtactgg 24660 ttagctcata ttgtttttcc tcctatgggg ctgcaaatct cttcagctcc ttgggtactt 24720 tctctagctg cttcattggg gaccctgtgc tcagcccaat ggtaggcttc gaggaaccac 24780 ctctgtattt gtcaggcact ggtggagcat ctcaggagac agctatataa ggctcctgtc 24840 agtaaggtct tgtactcagc tattaaaaag aatacattta tgaaattctt aagcaaatgg 24900 atgaaactag aaaatattat cttgagtgag gtaacccaac cacaaaagaa cacacaaata 24960 cgtggtatgc actcactgat aagtggatat taggccagaa gcttggaata cccaagatac 25020 cattcacaaa ccacatgaaa ctcaagaaga aggaagacca aagtgtggat tctttgatcc 25080 ttagaagggg gaacaaaata cccatgaaag gagttacaga gacaacgtgt ggaacagata 25140 ctgaaggaat gaccatccag agactgcccc acctggggat ccatcccata tacagtcacc 25200 aaacccagac acttttgtgg atgccaacaa gtgcttatgg aaccttttat ctatagagaa 25260 ggtttctcag ctttggtagc attaattaat tcgttgtcat ggaaaggtgc cgtttacagg 25320 catcccttcg cctctccctg tagttgccag aaacatctta gatatggatc aatatcttct 25380 ggggccagga gatattccca aattgagaac cattggtcag cactctgtct atcatgactg 25440 caaacaaatt gaaaacttga caaacattaa tgaaaacttg ataaacatta atgaaaagaa 25500 actcagaaaa cactttgata gtattttctt taagacatgc catgcgtggt ggcgcatgcc 25560 tttaatccca gcactcggga ggcagaggca ggcggatttc tgagtttgag gccagcctgg 25620 tctacagagt gagttccagg acagccagag ctacacagag aaaccctgtc tcaaaaaaaa 25680 aaaaaaaaaa agacagggtc tcatgaaacc cagactggcc ttgaactcac tatgtagctg 25740 tactgcctgg gtttgtgtgt caacttgaca caagctggag ttatcacaga gaaaggtgcc 25800 ttccttgagg aaatgcctcc atgagatcta gctgtaaggc atttttttca attagtgatc 25860 aagggtggga gggcccattg tgggtggtgc tggactaacc tgggctggta gtcctggatt 25920 ctgtaagaaa gcaagttgag caagccaggg gaagcaagcc agtaagtaac acccctccat 25980 ggcctctgca tcagctcctg cttcctgacc tgcttgagtt ccagtcctga cttcctttgg 26040 tgatcaacag cagtatggaa gtgtaagctg aataaacccc ttcctcccca atttgcttct 26100 tggttatgat gtttgtgcag gaatagataa cctgactaag acagtagcca acgatgatgc 26160 tgagcctctg attctcctgc ctctaccttc tgagtactgg atttacataa gtgagctgtc 26220 acaccagttt atatggtgct ggaatcaaac cacaaaggct cccagcatgc aaggcaagca 26280 ttctctcaac tgagctacag cctcagtttt agcaatgcaa agaagaggaa aaggagggag 26340 aagatatata tatttttcat tacatatatc tcacatatat tttacattac aaatatctca 26400 catatatttt aaagcgctgt agtggtgaga tattgggctt tgtagacacc aggcattctg 26460 ctgtcctggt tcttcaggct ttatgatctt gaacaccata gttctatttg gtgagtctgt 26520 tttctcagga gttgttgact atgcctttag tccagtgcta tctgaagaat gaagatagta 26580 tctctctcag tacacctttg gcaagggaag ataccatctg cccagtatca tcatgtacat 26640 tggttgagag ttaactctgt gctaagtgtt gtggtaaatg cattatttct tattccatcc 26700 tcatggcaat cctaccaaat gtcaaaaatt attatccaca atttgtagat aagaaaacca 26760 aaactttgac acattaaatg actgtcttag gattttgttg ctgggaagag acaccatgac 26820 caaccaaggc aactgttaca aagggtaaac atttaattgt ttctctgagc acttctgtct 26880 tccaggattc taccacaatg gcctttctgc tctatttaca gtattcaact gtttttttag 26940 tacaaagttc caaggtcgtc cacagtcctg caaaaagcaa cacaatcagg tacttcccag 27000 caacggcgat tcttgagtat cgatttctgt attatttaat cttctgttgc tgtgataaaa 27060 tgccacgact aaggcaattt acagcagtaa gagtttattt gggcttacat ttgcagaggg 27120 ataagcgtct ctcatggtag ggagattgga aggcagtgag tggggcctga cagctggaat 27180 aggaagcaga gtgatcacat cctttactgt gaacaggaag cagagacagc aaacagaaaa 27240 tgggctcaag ctaagaaaag aactctcaag tccagccgcc agggatgcgc ttccttcagc 27300 aaagatgcta gctctccaac agcatccacc atctggggaa ccaagtgttc aaacacacgg 27360 gtctgtgagg aatatgcttg accttaacaa agctagggaa atgtgtagac tcctctacac 27420 agctggaaga tgttcactct ctcttctttc ctttaaacag tttcttaaaa attatataac 27480 ccactcatat tttcctaggt gttacaatca taccttacct ttaatcacat tttcatttat 27540 ttgtttgttt gagattttat gtttaattat gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 27600 gtgtgtctgt gactttgtgc acatgaatgt ggtgcccatg ggagccacaa gaaggcatca 27660 gatcatttgg agctgggaaa gcagagccgg aggcagggac atctggcaga gttcagagtg 27720 gacatgacta gactgagctg ggtcatgtgg ggagatgtgc agaggaaggg aaaggggaga 27780 gatgggaaca aggtgcggca ggcaagagag caaagataca cacacacaca cacacacaca 27840 cacacacaca cacaccacat atgtacatat acacatacac acatgcacgc acgcacacac 27900 acacatattt agatgcattg aaatcatttg atatacaaat agaccatgca aagatcaagt 27960 cagtgtgatg gacatttcca gctgtttaaa ctttttaata cttgttagta ttgagagtcc 28020 cacccccacc ccatttcact gctttacaaa gatacaaaac attgttctgg gcatgttggg 28080 tgcattcctt aatcctagtg atgcagaggc aggcagatct cggtaagttg aaggctatcc 28140 tggtctatac agtgcattcc acgctagcta gggctaccag tgagattgtt tcaaaaaaca 28200 aaagtctgta gttatcttcc tatattgttt attcttctct gccttcctct ttttctgcac 28260 acctgtcttc tctgacactg gtcagcatgt gtgagtgctt gggcccgtta atttgtgtga 28320 gcagacatgt gtcaacatca cttagaggaa aggtggcaga ctcattaaca cacacgatta 28380 acatgattag gaccagtttg aaagcagcag ttctccatct tcctaattca ggcctgactg 28440 tgtcaggact ctacactctc tacactgttt tctcagctaa cttgtccttc ctgtctctca 28500 cctcttcccc acactgacac acatgtatac acagcccccc caccacatac ctaccccccc 28560 cctacaccac agacttccag agagagggag gtggtgacta cgctgtatgt gtttaactgt 28620 tacctgtctc tccttagttc tgaatgaaat ggaatctaat cttaatcctt tcctgtcatt 28680 attagattca tggagtccta acggccagag tctctagatc ctgtcttatg aaattcaact 28740 ctcatacaaa attcaaagta tcagtttcag agaaagttat ttgaaaaaaa aaactgtgga 28800 gttgaaagca attgaggctt attctggtcc tcttactcag tgtgtgtgtg tgtgtgtttc 28860 tgtgtatgaa tgcatgtatg catggggtat acatgcatgt atgcatgtgt gttcatgttt 28920 ggtgtatgca tgtgtgtatg tgtgaggtat gtgtatgttt gtgaggtgtg tgtattgtgt 28980 gcatatgtga gcacatgtgt gtgtgtgtgt gtgtgtgtgt gtgtatgcat tcagagacag 29040 agtgtgctca gcttccagta gatcctcagc taggaggtgg tatcttgtga gactcttccc 29100 tctgcatgct gaactgttga ccggcttgac ctgtgaaggt cttgtgcagg caaccacagc 29160 tgctgagagt tcatgcatgc aatagtcttc atgtttattg tagttctccc taactctggt 29220 tcttaccatt tttctttcta gtctgtgatg ttctctggat cttgcaggac aggccataat 29280 gcagataccg agttttattg atgtcaatgg tccactgggc tagcagcctt actgactctg 29340 ggatagatgc tgttaaatca atgacagtgg ctttttaagt tacatccaga cgttaggcag 29400 gaatagataa ctcagatata acacgaaggc ttacccctgt gtttttgatt ttaaaaaatt 29460 gtgagtgtgt tatacgcagg catggttatg cacattgatt tgtgtggttg tgggatctta 29520 tgtcagaggg acttagatgt cttcctttat tactaccgat gttattcttt tgggacagga 29580 acctggactt catttctttt tgccaggaag tcagaaagcc ccagggatcc tcccatctct 29640 tccttcttaa tttctgggct tctgtaggca tgagcaaaac tatgcctggc ttgttaggtc 29700 ggcactggta tctgaacctt tgcttccatg gttgtacagc aagtgttctt aactcatgag 29760 gtcccgattt ttttgtttta cttaatgcta tgttgtactg tattatatta tgttttgctc 29820 tctctctctc tctctctctc tctctctctc tctctatata tatatatata tatatatata 29880 gactggcttg caccatggag tatgtgtcag tcaggagata ccttttggaa gacagtgcct 29940 ttaaaccatt gaaccatttt tctgcccatc acaaattctt tggactaaaa aataagagtt 30000 tctttttcct taacaggaca aaactttatt ctctgatatt taaaatataa gagaatattt 30060 caaaatttca aaaaccagtc ccttaagtta aatcaagtgc ctgctccttc acatctctct 30120 tctcttttga tgtaagcact tattgatcta aactcacttc ttgaaaactc ggagcatctc 30180 acaggcttcg ctatgttgcc tctctacttc catttgcctc aagataattt ttaatttctc 30240 ttttaactat ttcattagcc tattgattat tgaggaccat gtcaaatttc ctaggttttg 30300 tgcccccctc ctcaccggag actttgaaag aatgtatgtg ctgcaaatga caacttagtt 30360 tcttgaagtt tattaatttc tttatgcata tgtatatttt gcatttattt tcactgtttt 30420 gctcaatgtt tgtcattgtt ctagtttcct ttctgttgat aaaacactct gaaaagcaac 30480 tttgaagaga aaaggacttg tttagtttgc tcttccaggt cacagtccat tactgagaga 30540 agtcaaggta agaactctag gcaggatgcg ggagccgtga gggagtgttg tttgctggct 30600 gactctctgg aatgttcaaa ctagttttat tatatagttt gagcctgcta gtctagggat 30660 ggtgctgccc acagtgggct gggtcctccc acatcaatca atcatgacat tttctatgag 30720 acacggccac aggacaatat gatcaaagag atctctcaat tgaaaactga aaaaacttcc 30780 tcttcaggtg aatctacgtt gtgtcaaggt gacaacttgt ccagtagtac taagcagggc 30840 agtcatcttt aatcagagag atgacagtat atattccctt actgtatggg tttgtgttga 30900 gacacctata atggcagaca gattaagtct tcctgcatct gctcagagcc cctgtccaaa 30960 cagttccttc ttccctcttg tctctccttc ttcagacagc ctacacaatt cataagcagg 31020 agaaaacatc cataggatgt tttccagtta gtgttctaga atttcagtag tagaaaggat 31080 ataaaaatta cttagaaaca gggagtactc aatggcaaag tgcttgcctg gcatgcatga 31140 agccttgggt ttatcacccc cccccccact cttagtccat atttatcatc tttcatttaa 31200 ggaagcttaa aataccagag gactagagag atggcttatt ggtaaagagc acttgttgct 31260 cctgcagagg accagggttt ggtttccagc acccacaagg tggctcacaa ccatcagtaa 31320 ctctagtcca ggatgtttga catctttttc tggcctctgt gggtactagg cgcacacata 31380 gggcatatac atacatgcag gccaaacaca aaagtaaaaa taaaaaatat tgtctaaaag 31440 ctatctcaga cacagggaag tgcagagatt tatcctaaac cacactgttg gttaatgtga 31500 aagtcaagct aagataaata aataaataag taaattaatt aattaattaa taagtaatag 31560 agggttggag agatggctca gctatttagg gtgcctgcag ctaggatgca tgttttgttt 31620 ccaggggatt gggggcatga gagaaacctt acaacctgct acagggaatt ggacatgctc 31680 tcatggcctg tgaggatact tatacacaga agacattctc tctctctctc tctctctctc 31740 tctcactctc tctctctttc tctttctctt tctctttctc tttctctttc tctttctctt 31800 tctctttctc tttctctttc tctgagacaa acacacacac acacacacac acacacacac 31860 acacacaaat aaaaacaaat cttggcttat ctgtgcatcc tttaatttca tcactaggaa 31920 ggaagacaga tctttacaag ttcagggtca gcctgatcta caaatcatgt tttagatcaa 31980 caaaggttat gttatgagac cctatcctgt ctcagagaaa caaaacaaac aataacaacc 32040 gctcccccac tcttatggta aaatgaaaag ttactaaagg taggacaccc aagagcacac 32100 gggacgagga tttctaggca agcatctttg tgcagtgttg cacctattat tgaccagcat 32160 tgtttcagga aatgttaggc agaatcaccc cgctgttctc ggaagcaaaa cagaggtgtc 32220 acaccttctg attagactgg ccagcttgct tttcaaatca tctcttcttt atcatcaaag 32280 agagaaggaa acctcggaac aatgctgtct ctgcaatgct atccccactc gatcttatcc 32340 aaggctgccg tctttgttgc catagccacg gagacctgat gcgcaggtat ttgatcttgc 32400 tatgtgccac tgaagggtgg atattccatc ggagaccaaa gaacaggagg ttgttaagtg 32460 tgttgtaggg aatcagagcc atggagaagt tgtttgatct gagtccaggg ctgagcagga 32520 cattggtaca tcctggatga aagctttacg gtttgtacac agcttggaga agtttaagtc 32580 ttggaaaagc cgggcagatc ccatgtgtta actcctccat tgctgccagc ctttccaagc 32640 ctgtttcact taacaaatat ttattgagcg aggtcattac ctccaatttt cactacggag 32700 acttgaaaat taagacacgt actctgcctt tgaggacctt tgaggcacac agatgtaaac 32760 tggccattgg ataatatcat acatgacacg tctgagacac gtgaggggaa agtagaaaag 32820 ggtctatgtc tcatacagca aatgtataaa gttctgtagg taaagtgcca tttgacatgg 32880 ctcctaaaag tccccaaaga tagatttatt tatttattta tttatttatt tgcacatgaa 32940 taatcaactt gttgaaggac tgagagacca cagaaaaaca agcaggagga atagcgacca 33000 tccaggaaag aggcttgact gggcagaagg gtcaaactcc aggagacaga agacaatttg 33060 aattaagatt agccaatctc agagattcct tatgaatggc ttgatgataa ctcctcagtc 33120 ttagccagtg ggaagttttc taggaggaat tctgattggg cagatcttta gccacatgca 33180 atgcccgagg gtagaagatg tgtcttctta ttctgggcag ccacactgtc agctgaaaat 33240 agggtgtgtg ggttgtcatg tggacagtgg cagttcctat aatccatgcc tacctgtgct 33300 gtagctgcac tccttgactt acacacacac actctctctc tgtttctccc ttcctccccc 33360 ctctctccct cccttccctc tctccttttg cagaggtggg tattgaaccc ccgacctcgc 33420 acgttatgat agacaggcac gtatccctgg gcgctatgct gtactattaa cgatttgtat 33480 ccctttcctc tggagaggag atggcgggct tttcaaaaga aagaaagaaa tattcttact 33540 ctatctatag tacccaacag tgtctggtac acctacatac acagatatgc agggcccgtg 33600 gaaacacaac aattccactc ctgtttaggc aacttttcat gctctttttt tccctctgaa 33660 cttggcttca tacagttttt agtgttcaac cttagtcttg ttaaatccac tccaccatag 33720 ctcagcctaa tcatcggctt ttgaatggat ttacagaact agacacacat ttcctcctac 33780 agagcagacc ttacgtccca tcagaaagca gttggttaca cccaaatgtt gtatggccct 33840 ccattaaaaa agatgagctt atttgtttta tgtgtgtgag tgttttgctt gtatgtatac 33900 atgtataact tgtgcatgcc tgatgtctgt tcaggtcagg agcaggtgtt tgctggatct 33960 cctggaactg gagatataga tactatgacc tgctatgtgg gtgctgggaa tcaaatccaa 34020 gttctctgca agagcagcag gtgttctttt tttaagttaa tattttagag aatttcatac 34080 acaaatactg ttatttacct cattaccacc ccactcaacc tggatcctac ccccaaccac 34140 tcccttctaa aatttatgac ttcttcattt cttttttgag atttatttat ttattttata 34200 tgcctaggtg cttctatcta tgcacccctt acacggctga tgctcaaaga ggccagcaga 34260 gggcgccagg tcccctggaa ctggagttag agatgacggt ggcttttcct ccctttttac 34320 caggagacct agtcctcaca gaggttttca ggtcagttcc agtttgattc cttcaagtcc 34380 tgtgtccaaa gtgtgtgttg tctttagcca tacgcgttta atgtcaagtt ctgggaggca 34440 ccaaagagaa cagcagaagc cgctgggact tctctgacca acagcacgga ggtttttcat 34500 acacggtact agatagtttt tggctcttgc agggagcatt atagcccaag ctggtgtaac 34560 accatttaaa gcacacgtaa gcagtatatg catatactta cacttgtatt catttatgta 34620 ttatgcatgt gttcttttta gagatctatt ataggtgtat gaatgttttg cccacatccg 34680 tgtacatgca ggacatgtgt atctgatgcc tgagagagtt agaggagagg gcatcaggtc 34740 ccctggaact ggatttacag acagttgtga gcagccacga gagtgatgga gactgagcct 34800 gtgtcctcta caagagcagt tactgctttt aaccactcag cctcgccccg ctacgcgtgg 34860 atttttaagt aaacacgaaa taaacttttc ctttcgcctt ttcaaactca ctttgtctta 34920 tttatgcctc ttccctcttt tccctctatt ttattcttcc ccctcccatt aaagccccac 34980 ccccttttct attcccccct ccctttttgc taggacacag caagtgctct taactgagga 35040 gcagtctctc tagaaccccc gcccacgcca cacatctttt agagctgaaa atttcctaca 35100 gcttccgaaa ggaagaatca ggatagagtt tagaaatata accaacagag gagaagatgg 35160 caaaactact gctgccttca gcacagagag gctttaccta gaaaatgggg ggggaaatgg 35220 aaacatggaa aaatgcattg attggaaata taccgaggac atgggaagtt ttaagggtga 35280 tgagcttgag gcgaggaaac cagatttgta cagttttgga agaaacatgc aaagtttaaa 35340 ttcggctggg gatcagaggc agttatttta tggcaaagat gagattggtt ttgtccagct 35400 tggcctcatt tgtacctctc cattttagct atagtcacat cttattaata cttacctcac 35460 tctttggagc ttaggcagaa aatccattat ttatggttga gtattcacca tttatgtgta 35520 agtaaggaca tctgtatgcc attgaatatg tggttgtgaa gtaattaaaa cagtttacag 35580 tgtaaaatga aagacatttc tctattttaa acatgcaata gagaaatggc attttatata 35640 ttgtttagaa agatttattt atttatttaa tgtatgtggg tacacactgt agctgtacag 35700 atggttgtga gccttcatct ggttgttggg aattgaattt aggacctctg ctcgctccag 35760 ccaaccccat ttgctctggt tggccccact tgctccatcc caaagattta tctattatta 35820 taaataagta cactgtagct gtcttcagac acaccagaag agggcgtcag atctcattat 35880 ggatggttgt gagccaccat gtggttgctg ggatttgaac ttaggacctt cggatgagca 35940 gtcagtgctc ttacctgctg agtgctgagt catctcacca gcattttata ttctaactag 36000 attcaataga cacttttgtt ttgttttgtt ttgtttgttt tttgtttgtg atgaagaata 36060 ctaaggggtt tttacttagt tagcagtaag caagatgaac tgaatatatt gtatcaagag 36120 gcatcgagat tgcatggctg taactcaggt ggtgatgaag gaggataacc atcccctgga 36180 ggctaacctg agctacacag caagacccaa cccaaaagca gccagccaac caaaccaaac 36240 tcaaacccaa aggcccgtga aaaattgaat tctgatgatt agtgccccat aaggacctta 36300 tggaacatga aaacaatagg aagaacaaag agaacgagca ggtcagaacc acacttaccc 36360 aaattaaagt ctcccagctt ggaagatcaa atcatacatt acctcaagta actcatattt 36420 gtctgcaagg tgtcagctga ccccttttct ttaatatctc acttcccacc cccaccccct 36480 tttaaacaga aagacctttg attgtggtgg ccaataattt gaagttttga agaattaatt 36540 ggccctatta tacatgaaga catagtaatc ccccttcact tttggaagcc tcggagagag 36600 tttgagaatt tcaagttgaa atatcacccc ctttcacaaa attctgtatt taaggatagt 36660 atttttcaga atttaaaaaa attttttact ggaaacttaa tattacagca ttccccttca 36720 gggtttatgg tcaagctttt ttaaatatat gttttatttc tttaactggc agatgtttga 36780 aagatgagag taggattaat cctcatatta atttttaaat acatctcatt cttccaaaga 36840 atgccgagaa aaagaaccaa ctctccatgt agtaactggc aaggaaggaa atagggctcc 36900 taccatggca actccctgtc agggatgctt agtgttgttt ctgtccaggt tcctcactgc 36960 tgagaacaaa tagccaacaa tactgagaga cggggaggtt tcttctggct aacagtttca 37020 gggatacagt tattgcagga gaggagagga gaggagagga gaggagagga gaggagagga 37080 gaggagagga gaggagagga gaggagagga gaggagagga ggcagctggt taaaccgggt 37140 ctgcagtcag gaagcagggg agccacacat aaatgccgag actacacttg ctttttcctt 37200 ttgctccact gtgagaacac agcagacctc aaaacacagc agtttgggaa ccaacacaca 37260 cgtctctttg tgtctggcaa ttgatgtaag ctccatggtt tttgcttttt ttctagctcc 37320 tttaattgat tgattgattg attgattgat tgattgattt ctgagactgg attttgctct 37380 gtaagccagg cttgtgtaga actccaattt tggctggcct ggaattggag aagttcatat 37440 tctcattata cagagtgctt aatggcaccg ttatgctcag gtcattcatt cattcattca 37500 ttcattcatg catgcatgca tgcatgcatg catgagatag gagttttctg tgtagctcag 37560 aatttccctc caacttcaaa ttctccttcc tcagctttcc tggcaatgaa aatacaggcc 37620 tgcgccatta tgcgcaagtt atattggctg ttgttaatat ttatgttagg aaaagaaaat 37680 agtctctgtt ttacatacaa tctacttgga atcatttttt ttttttaaag ttaagtcggg 37740 gtgaggggtg tgtgcaggtt aacaagcaaa cgcaattcct ccagctaacg ccaaggggca 37800 ccctctcctt cccctcggtt tctggagcgc cttgagttct ttccatctgc acaaagagcc 37860 cggcctcctc ccgggtgaca tcacaaaggg tcgttctccg ccccttcggc gccaccacgt 37920 gtgtccccga gctgagtggc catccgactc agtccctcgc cggccggtct aggcagcgga 37980 ggaggcgagt gcttgatagt ggctggaagc ttcgctatgg gaagtcgctc tttagcactg 38040 tcgcggctag ccctgctctc tggttctccg cagccgctgt cgttggagag caccgggagg 38100 cgcgggttgc gagcgcgcct gcttctcacc gcccgggcgc cagcgcccct gggcaggtgc 38160 tgagcgcctt tcggagcctc ccctgctgcc tccctcttcc gcctgggtgc ctggctgctg 38220 cagtgcacat gggctgctgg aggtagatgg gctcaccgcc cgtgaggcgg cggtggatgc 38280 ggcgctgggc agaaacagcc accaattcca gctgccgtgg ggccgagcgc cccgggcgcc 38340 gctgcgagcc ccgagctcgg ccatggggac ccgggcaagc agcatcaccg ccctcgcctc 38400 ttgcagccgc accgccggcc aagtcggagc cacgatggtc gccggctctc ttctcctggt 38460 gagcgatagg aggggaccct agggtggcgg ctacgttcga gcctgtgtcc gggagccggg 38520 cgggtcctcg ggcccgtagt gccggaggcg cgcggtggga ccagacgggt tggaagggtt 38580 ggttacaagg caatagccag accgaggagg actcctacta gatcgtgtgt gtgtgtgtgt 38640 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgg tgagctaagg ctagtgagtg ttaaagaatt 38700 atttttcttg caatctggct gcttgaagga ggcgcgagag gtgtccgcct cgaggtcctg 38760 cgcatttttg ggtttttgcc cccgactcgg cctatctcgc accccaacac ccagcctcac 38820 cccaatatca ttcgctgcca gaccctcggc gcggtgcgag gcccgagtag tcctgtcagt 38880 agccagctgc cggttgtggc cgctcctttg tcgctactgc aatgtcatgt ccggatgcta 38940 ccgcagtgga gacagccgga tcggtgcgct ccggacgcga gcaaccgctg catgcttccg 39000 cgcgcgaagc tttccggagt agggcgcagg ccaccccttg cttcagcttc cttgcttctc 39060 tcctccctct tagttcagat gggtcctgat ttctgcaagc caaaagaaga agaagaagaa 39120 ggagcccggg tgggtggtgg gggtggggtg ggagggggag gaggagaaga aggacccaga 39180 gacccaggcg cctctaaagc acaggcttgg agccaaccgg cgctgtggct ctcagtcgag 39240 ggggagggga aagagctaca taaaggacga ttaattttcc ccatcctttt cttatttctc 39300 cctttctgtg tttagacatg tcaccaagca agatgatgca ggctctctca gcacccaaat 39360 ctggggcgtg cccggattgc aatcctccat cactttatct gatcaaaacc tgacatcatc 39420 ccttatccaa aacttacccc cagctggccc tttatttcca atattggggc caagtgggga 39480 attaggtgga agaaaatttg aattcaaacc cttgagatgc tataaaccaa tcatgtcttt 39540 catccctgca gggaggatga aggttgttag ctaaaaacta aacaaaaaca aaaaagagct 39600 tgttttcatg gttgggctgt gtaagggaag gcaggcctct tgtctccttc ctctaaaggg 39660 cttactgcct ggccagccaa gatctactgc ttccatccca aggcatgaca gttgctatgg 39720 cctgggaatt cattctacag acattctggt tctctctgtc tctctctgtc tctctctctg 39780 gctgactctc tttctgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg 39840 tgtgtgtgtg tgtgtttagg gagagagaga gagagagaga gagagagaga gagagagaga 39900 gagagagaga gggctaaaga aagtttgttg ttcttagcat ttatgaggta aatctcagtg 39960 tcccttgaga gacgcttttc tgtcttttta taacaatggc aaagtccaag ccctttaaac 40020 aagtgtggat agtaaataat cagagagtct ttgggaccgt ggacttgaat tgacagtctt 40080 agattgaacg agttcaagga agcactgatc ctaatgacct gagttaggtc ccttgctgag 40140 ttccctccaa aggtcttaga cctgcttccg tgatgagcat atactgccgt gtgtccctgc 40200 tccacacaga tacaatcctt atacaatact tatagatcag tccactcaaa tccaatctaa 40260 agttagtgct caatgtatag tcgtaatctg gaattatttt ttaactttta gcctgatata 40320 taggtccttg aaggtctcag catagtaaaa ccatatgctt taagtaacca agaaagggcc 40380 tagttctttt tattaaatga tcaaactcaa tgtacccaca gattgcaccc agatttgcaa 40440 ctgatgacct acagtataag tttgacagtt tgatgaagtt tatgaaaggt attttagaga 40500 caatctcaag gtgacgatgc tacagggttc cttgatttcc tggtaactat ggcaaaaggg 40560 ctttctttaa attgatggag taggaggagg gtgagctgac catcctggag tgtgggaata 40620 ctccaggaaa catcaggagc tatgcaatga ccttctccat ccccactttg gacggtccag 40680 tcatcaggac gggccctgtt tctgtataca acagtgatct ggtgactctg ttctcaggag 40740 aattggtccc attggtaatc ttggccacat taacagcaga caccaggtag gacagctcaa 40800 gtaaacatgg tgtgaggtac ctagaagaat aatgggggat acaggaaggt taatgctatg 40860 tatatacagg gtggttagat aaagaaataa tgatggcttc caattgccct tctctcattt 40920 ttttttcata gcagtctttg ttgttttttg tggctctttt ttttttctat cagcatgaag 40980 agcttactat aggctctgat atgtatgcct atcatatgaa cttgtcaaaa gagcagcgag 41040 ctaatttata cagaatatgc cactatccct gtcaaacggg tgtcacctcc attctacgga 41100 tgagaaaata gtctccaaga atcttactgg tctgttgtag tatgtggtga ctctgggctg 41160 gaactccttg gctcctactc tctctcaccc acgtaccaca ctgcccgttt cacaacttca 41220 agttgtttgg tgagtgtaag gacagagaag gactgctggc aggtagctga gacggtggct 41280 tgattaattg gggaatataa gtgagacgtt cacaaggacc tttggagtga gtcagtctag 41340 ttgctggcat ttctattgct gatgtgttga tgtgaccgtg tcatgtttgg aagttactca 41400 caaactgaaa tgcagcatac acgttccagg ccaaagttct gtaaatgaga catttagatc 41460 tgtagtggtt tagggattgt cctagaagga cttgcttact gcaatgctgg atgcagtcag 41520 aggtgcctct tgcctttagg gagcttggtt tgactataca gatagactga ttgcaaggga 41580 tggtcaaggg aggaaagcaa cttccttggt aaagactttg gatttggagc tttggaaact 41640 gctgttatcc acggactgag aattcctgca ctgaaggcac agggaagaag gcatagcaga 41700 agaaggagtg gaaagattct aaaagccaga ggaacagaag ttgctgtgag atcgtgtcac 41760 ctggaaatgt ccgggaagct tcactcatga agtttcaaca gcatggctgc gtaagcggga 41820 tctgatcaga gaacaccagt ggacaggcta acacagaagg gggaacttca tagggcctca 41880 gccctagaca aaaaattaga aatatgggct actaaggaat atatatgtgt gtgtacaata 41940 cgtgcatata tatacaaaca tatgcacaca cacatatatg tatgtgaatg taacacatgt 42000 gcatatgtat tatgcacatg tgcacatatg taatagcaat tgaaaataac gccatgaatt 42060 tgagtcgtgc aaggctggta catgagaggt tggctgtcga gaggaaaggg aaggaagaaa 42120 agatttccac acaagaggtt gtccagctgt ctggccatcc cttttctagg cagctggcat 42180 ctcaccattt ctggtcacca aaagaaaaaa taccttattt atttatttat ttatttttct 42240 tttgccggtg ggaaatggtc agacacatca tcagacacat catcagacac atcatcttgc 42300 ttagagggtc ccaagtgtta ctgttggcca ctgctcctga gttctacctt ctgacttcca 42360 actatcacag aaaatggcta tctgcctggg caggagtgta catgtgaggt catctggggt 42420 ggggtggggc actcaagtcc tagccttggt gacaaatcca attagaatat gaaatcagag 42480 ctggcgggca tacagagggc tctctccctt tgatatactt taacacagca tcctagactg 42540 tggaagctgg gagggaagct aagctgttac tgatctaaca ccccacacag cttggctgag 42600 ggaaagaaca gcctccactg cggaagtgac tatttttcat tttaccaaat ctacatggtg 42660 cagaggcggg atcctaagca gtggaagccc agtgatgaag tgggattaga accttgcgtg 42720 gcatgaagct catttctgtg gcttataaaa caagtggccg gtgtcaatgg atcaatgggt 42780 gagcatgcta gaccccgaga gagacacaga tgtgatgttc acatcttcaa aatatgtagt 42840 ctgtcagagg aggaaaacac ttctattttg gaaaactttc caatcttgga atgatatatg 42900 ctagttgatt tatgggaaat tgttaatgag gaggaacaag taatccagat cttctactgg 42960 attgaacaac tcctgatttt taacccataa gttatcccca caaagtttag agtagggtca 43020 agctttctca tttggactta ctgtatatct ctggctatca gatttagtca cagacagaca 43080 gacagataga cagatagaca gagtctccag gacaactaag caattaagat gaatctacaa 43140 gaaaaacctc tgtagttaca cgggcacagg tccactgact acttatacat ctggacaatg 43200 tctaccaatt agataaagca accatagttt gccttcccca gtggtcaggg gtgggtttgg 43260 cttcagagct gggtctagtg agtagctggt gtcttctctt tggaatttgg ggacatcaca 43320 tcttgacttt tgcacttgac tccatcttga ggagaggcaa agcttcttgg gaggcttggt 43380 agaaaagctg caaattggct ctgtgctaac tcagccagca ggcaaactat aattggctta 43440 cataaagttc acgattatta ttattatttt tttttctttt ggtgacggga aacggtgaga 43500 tgcatcatca gactattcaa gggccaggac gaattgagcg acttttcctt gcgtaaacag 43560 ctctgcctat tttccagaaa gcactaatac tctgttggct tgcttccccc ctgcacaggc 43620 ctggcaaatt gcggtaatgc caggtcacca ccagcaactt cttggcaatc tcaggtgctt 43680 ctcttctgtg actagaaggg cccagattaa ctggtgggta tccggtggtc atttggagtt 43740 gagtccattt ggagcacgag atgcagatat attggatgga cagaatgaca actgaagcca 43800 ttagtaggag cagcttggcc cactgagtgt cagcaggagg ctggacttgg ttgtttctgc 43860 attagaggcc actgagttgt gttctttagg aatagaggcg agagatggtg gataaacact 43920 ggctttctcc agtgtttata tttgtcaaat acctaaaaat caacaggatc ttaaattggc 43980 ttgaaggctg ccacctggtt ggacagtgat tattaacctg gagggagact ggcagatgat 44040 cagaaatatg ggtggggcct ctgcttatgg agagaggttt tgatgaccgt gctgttgaca 44100 gaaggcagat gcgcaatcaa cattcatgaa gagtgctgaa gtcggggtcg tgtgtgtcct 44160 ggttggaggc aacatgtcta tagtgttagg aaagttgtgc tcagggggct ctcaggaagg 44220 tgtgggatgc agcaggacct cggtaagctg gcaggatgct gccccatgtc ctttgatctt 44280 ctcttcagaa tggttgcaga ctatgcatga ctcgtcgcag gtctaagaca aataaaaaga 44340 attggagaga agcacatagc caaatcagaa cacggctagt agagctatga gacaggccta 44400 tatcgtgttc cattcgctct ctgcaaccag gatcactgta gtggctctgt tgcttggcac 44460 catttgcaaa atctgcttgg agggaagagg aacacaggct taatagagtt agacaagaac 44520 atggagcttg tccatgtgcc ctggcctcaa gagagcagct gtgaaatcag cagagctcct 44580 gtgttagtgg cctttggagg aggactaagc aaatggctgc taacccttta tattgggatg 44640 ttatttaatt tatatattat aaaaatatta tgtaatatat tcattcatat actatagaaa 44700 tggttcttat tactgcattg aatatcttct gtaaagcact acagtgtaca ttttacaagt 44760 cccagacatg acagctttag tcccatttta cagaatccag aataaggttt gatagagttt 44820 ttgctcagat atctgcccat tattacgttc tggagagagg gatgaaatct agctcagcat 44880 agtttcaaac acttagctct ctctgccaag agagaatgga acaagtgaac agaatggttc 44940 acttcaactt cccatacata cgtttgttta ttctaaaggg aatggtttta cccaagaggt 45000 gaacatgccc gttttattct ctgagtttca cagtggtacc tgaatggttt gccaaaacta 45060 ctggtacttg tgagcatggt gtgtgggagc tgaaagggga gggcgtggag acattgggcg 45120 aacactttcc tatggtgtga ctctgacgag ccatggtgcc acggttgaga tggaaagaca 45180 ccccagcaaa ttgaaacttt ataaataggg aacgggcgtg ttggctctcc tgcagccctg 45240 ggtcactttg aaaaatattt gcttaatgag taacagcgag gtgtgggctc cactctgctg 45300 tgtagttagt ttctgctcct ttagtctgag cacctgagga cctgctaggg tctttgctgc 45360 tagcctgact ctttgtatag gataatagct gtaccagcaa tgactagcat ctgtccacat 45420 ttcattccgg ttctcattcc atggtttgag tggttcgtgc taggaatgta gtggataggt 45480 tgccacagtt actggagcac cctcataaac aagccgttag agaaaaaagg agagagcttc 45540 atacatgcat aggatacgtt ttgatcaaat ccacagccct tcactctctc tctccccttt 45600 caacttctcc caacagcccc ctctccccat cacctttccc tcccaatttc acgtgctctt 45660 tctctaaatc cactaagtcc gtgcacctgt aggtacgtga gtgtaggact atctactgcc 45720 gcatggatag cctgtcagga gccccatccc caaagaaaat ggagtcttcc tccccgaaca 45780 gccatcaatt cctaacggct tctcagctag gggtaagaat ttatgactcc atctcctgcc 45840 cacgcaggct tttgactggc ttggttttat acaggtcttg tgcatgcagt cccaaccatg 45900 cacacttcat gtgtgcaggg ccctggcatt tctagcaaat actgtttcac tctaatcaaa 45960 tgatcctttt ggatcttaca agttttctcc tcttctttga gatccctgag ccttgtgggg 46020 gaggggctga gatgcatcgg tctcattttg cacctaacac tccaaggtat tctaggtatt 46080 ctattctctg tccgttgacc aggcgtaggt ctctctctct ctcttttttt ttttttttga 46140 ttcccatctt ctacactgtt ggaaggggag ggagctatgc tgattaaatg agctttgaga 46200 tctgttgaaa tcccaatttg tagccttagc agacaaaagc tggaaggaaa tgatgtatta 46260 gtgccagaaa agagattgga gttctggtct cccaaaaacc tttatgacaa gaccactgtc 46320 tgatttcact gcagacatgg ctagctagct ctgctcagca aggactcaga gaggcagcgg 46380 ggtacgaacg agacaaggaa acttgcaggc aaagatgaca ggttttcaga acagtccaac 46440 ttgtatgttt gtgcttattt tggccccact tttctttcca taattatttc cctttctcct 46500 acaggggtat atttgtgttt ttcatcaact tactaaagcg taaagactat ctactaagga 46560 acaggcatgg ctagggtgtg ggtctataga agtgactaca ggttatctgt agtctcccct 46620 tggagttcgc agaaaacgtg atgtttcatg gtaatcatca taaagcagaa gggagtggat 46680 ttcagtggaa gggaccggta tgtagaatag ccccagtgta ggacagagtc tgtgtcttca 46740 tctctgacca ctatgactgg gaagaggcag tgggactgtg tgtcgtggga cccttgaaac 46800 tatgaagatt cgttcagtgc aggagaagca gggtaataac atggcttgaa acattctttt 46860 tttttaaacc ttatgtatct ccagatctca catctccaga aagttcctgg cagtcttgag 46920 acactaatat gggttctggg aaccaaatgc aggcctctgg aagaacagca agtgcttaac 46980 cactgagcat cactcccacc tgccaacatt gaaaaaagaa gttttaatta atccttagag 47040 aattccattt agtgtatttt gattccattg ctcctgaagc tcctcccaga tcctccccac 47100 cgcttaactt acccaagttt ctctccctct cgcgcctcct tgcattgagt atagtttgtg 47160 ttggctaact gcttttgggt atcaggccac aatcaataag gaaaaaacaa aacaaaacaa 47220 acaaacaaac ctgtttctca tactgaggat ggaacctagg gttttcacat attgagcaag 47280 tgctctacca ctgagctgta ctcagctctc agatcgctcc agaagcctgc tggaggatgg 47340 aagctggagg tcagctgctt tcacagggtg gagatcacag tgccggggat agtggcggtg 47400 aagatgaatt ttctagaatt tactggtggt aagatgggag ggggtcaggt ggtctcaaag 47460 gaggcggact cctaggtgcc taaattgaag tctgtgattg gaaggatggg ctgggggcgt 47520 ggcgggtaga attagaaccc taagcttctg ctgtgtagtg agttaagttt gaagtgtcgg 47580 cgatgaacat tggcttgggc cttgattttg ccattttgaa atagctgact ctctgcaggt 47640 ccgaagcgca ctgagccatc gcgactgtcg ccaggcattt gggctgccgt ccttcctccg 47700 cagcatccag cagcacagac atctgtgtgt agagcaatgc tctgttcccc cttaaggttg 47760 cttcctctga ttgcattccc caaagtggca ttgccaggtc agaggaaacg aatgagtggt 47820 ttttatggcg ttcgctgcag attgccagat tgttttttag gaagacaatc aattcagcca 47880 ccagccacct ataaatgtgc tttctagtcc atgaatctgc cgcgacggga ctttctcatt 47940 tcaaattacg tatcttcttt ttaattcagc gatgtaagga cctttggtgg tctctgtgac 48000 agtttagtca tttctagggg cataataaac ttacttgcat gatgcttgac ttttaatgac 48060 tcatggtcct tctagcgagt agaagttatg ctaatcttat ttttatcaat tctacttatc 48120 attctggtta taggttctta ctagctgtaa tggcattgct ttatattcta ttgacttttt 48180 acgatcatgt ttacgtattt gctttgtttg tgtgcacgtg ggcatctctg tgtcacagtg 48240 catggaggtc agagacaatt tatggagtct gtgggtcctg gggatagaat tctctacctg 48300 tcaggcttag tgttaagccc cattgcccac tgatccattg caccaatact gcaccactac 48360 tgtactctac tataatatca tgaaaaaaat aggattgtgt tattaaacct tttcctttct 48420 gggtctgaga atattctttt tgttttgttt tttggatttt tttttttttt tttttttttt 48480 tttttttgcc cattttgtta ggaattcatc ctttgacagg tagttataaa gaatcctaat 48540 tgctttgaat tttataatgt tattggttga cttgttgata tttctggata ttggtgtcgt 48600 atatgtggtg ggaatcccct tggtgtgttc tgaaggctat atgtgcgtgt gtgtgtgtgt 48660 gtgtgtgtgt gtgtgtgtgt gtgtgtaatg gattctgtgg gtaaacacac aggggagtgc 48720 tgggcccagc tgattcacca tttcaggctg ttcttccgtg gagtcacctg ggacaggtga 48780 tagctgatat attaattgta cccgttcagg agaagatata cggagtattt tctgaactct 48840 agactagggc atcagagact ttttctgtaa agtgctgggc agtacggtta tcttatgata 48900 tctgtggtaa ctacttaatt ccacctttgt gcaagacatg tgcagacaat gtctcgaaga 48960 gtgagtctgg ctgtgtttca ataacacttt atttgtggat aatgaaattt gagtttcaca 49020 cagttttcac tggtgctata ttgctactca ttcattcctc ctcatttccc agctgtcaga 49080 ctataaaact gttgtaggcc tgcctatagg ctatacaaaa atagacggcg atagagtcca 49140 gcctgctttt agaccagggc attaatattg tgtgagagca aattggggca actcactttg 49200 gaaaaccctt acttaatcgt gttctgggca tctaaagtga gattattaat tgtaaatcct 49260 ttctatttta ttattatttt ttgttataag ccaaggttta tttcatttta attaattaac 49320 ttactttgag gtagtgtctc tggttgacta ggctgaccca aggctaacct tgaattgcct 49380 ccgttttaag tgctgggatt atggtgtgtg ctggtctcgg aactcaccag gcaggcgctc 49440 aaatagctga gccagccaca tccccagaat cccaagtgtt tgcttgtttg tttttaatat 49500 agtgtgaaaa gtagccttgc tttctcttgt ctttatcttg acgtctgctt taaaacaaaa 49560 cttctacagt gtgctctgtg ttcggggaag tgagtttcag gattataatt ttttattttt 49620 taacagtatt attattatta ttggtatttt tgagacagag ttgctctgtg tagccctggc 49680 tgtcctggaa ctcactttgt agaccaggct ggccctaaac tcagaaatcc gcctgcctct 49740 gcctcccaag tgctgggatt aaaggcgtgc gccaccacgc ctgcataatc ttttattaaa 49800 ttaaataaaa gaaataacca atttttggtg tttgaggaag aatatcccta tggaaatcag 49860 cttgaatagg aaatcagtgt ctagcacaag ctggccttgg attttgtgat gttcccgtct 49920 ttccatccta aattctgtgg tcataagtat ggaccaccat gtaaataggc aagtaccact 49980 agtacatagg catgcatcca gatggcagtt cttttcttat ttgcttgttc tgagccagtt 50040 tgggtgtgga tccctgcctc actttgaata cactatgtat atccatctgg ccttgaactc 50100 actaagtaca ccaagctggc tttgaactca ctgcatacct caagctggcc ctgaacttgg 50160 gatattcctc atgcctttgt tcccaaagtg ctgggactac agtggatgcc actgtgatgg 50220 agttgggtta cacctttaaa caaaaaagtc tagagtttgg aggctggaaa tgtaacctgc 50280 ctagcacaca gacaaccttg gacttaattg tagcatgaca aaacaaaaca aaacaaaaca 50340 aacaacaaca aaaacaaaac aaacagcaac aacaaaaaca aaacaaaaca aacagcaaca 50400 aaacaaaaca aaaaaggaaa catttctcaa atttatactt aaaatagaat tgaaatgttt 50460 taaaacattg tctattcaca ggtaataaac ttaaagtcta gtaaaataat aaattggtgg 50520 ggtgataatt tcccaaactg gtattccatc cagcaattac catgtcagac atgttctcca 50580 tactccttct gtgtagctaa ttatcaaaac aacttagaat ggccgttgtt attgctgtgt 50640 ccattttaca agcgaggagg tagaggcttg gacgttggct ccttcccagg ttagaaagat 50700 agaaaaaaaa aatgtaattt tcttcagctt aggaaccaga ttcaccagct gacactcaca 50760 gggtgtcttc aattcacacc cgaccctatt tgcttcaaga tagctaccgg ttggaaggct 50820 agattccaca agacggactc taacggacgt caggtatcaa gagtaaaagt caggttataa 50880 cctgtgcttt ctgttgtctg actgtaagtc agagttccta tggctctcag ggggttcagt 50940 taacttgtaa gcatggcctc acatatctca gggaaacact ttctgaatat ggctctcttc 51000 tacagaaagg agacccatga taaagcatgc agaggaaggc atgcttaggg cagccatata 51060 aggagcaccg tggagctgtg gagctactgt gtctgtctgc cttggacacc gtgagctctc 51120 tgaaccttgt cctttcagag ttttatggag atgctatttc ataggcacaa tttactacgt 51180 cattagacac tggtgactaa acttaacctt caacccctct cctcatccca gagattgagg 51240 tagaactgaa agtagcaagc tgaatataaa aaccttgtgt gttctgacca ctggtctcca 51300 tcttggtggc accctctcag agcctccgtc ccctgtcatt ttatcaccac ccagaagacc 51360 ctaatcacgc tggctgtgag cttccctact ttgtaggatc catgggaaga gaccacatct 51420 atattcctta ttatcacagg atcgcggcca gcagatacct gggcgagcag ggcgtatgtg 51480 gtacattcta ggcttaaatg gtttttcctt atactttgaa gcctcagttg ctgcactggc 51540 ctgggttgta tgctcttcca gacatgccca gtcagcatga agttgcatcc ttccatcatc 51600 tactgttttc tatgacatac tatttcactt attactcact gggcatccta ttggcatctc 51660 tcagtaagca gagaataaag aaattactac tgtgttggag ggagacgggg ctgtggtcca 51720 gtgactgatt gctgtatgca ctttctcttc tgcattgcgg gttccagaga aaacagctcc 51780 taggtgaaat cttccctgtt tttttcctct ccagtgtccc ctgagcttgc ctccttctgt 51840 tcctacagtc agcacattct ggctctggca ttctctatag tcaccagctt gaaaaacagt 51900 ccctgcttcc tttgctttgg caataggaga cggctctccc tccttcccat tcctctcatc 51960 cctcccatcc tcctcttcct tcatctctcc ctctcttcca tccttccttt cttccttcct 52020 ctttccttct tttcagagaa ggcagtagaa atgaaagagc caaccaagtt tatataacat 52080 aactggaaat accctgtctg tgatttgtgg aacctttgtc cttgaaacgt ttgtttgtat 52140 gtatcccacc tgccccacaa cttcagatct ggggaattat agccatccag tgtttatatt 52200 acaaattaaa aatgatggcc cagctatctg gtcaacaatt ctgagggctg agcagctctg 52260 tctttgtcat caggtatcat atttcatagt catcctcata ggcaaacctg attaccttac 52320 aatctctaca gtaactacac acacacacac acacacacac acacacacac acacacacat 52380 atatgtatat acacacacac atacatgtgt gtgtgtgtgt gtgtgtgtat ggaaaaattc 52440 tatatcaaag tccagagcaa ttttcctgct tcttactaag gaaaagcagg tccacctctg 52500 tcttaagttt gctcgtttta taatgaaatg ttactagaac ttgagaggaa atgaaagtaa 52560 tgtctgcctg gtgcagggac gatggctcag tgggtaagat gctggcttac aaatgtgaag 52620 agatttgagt ttgaatcctt agcactcatg taaaaaggct ggatgtggtc ttgtgagcac 52680 ctgtaacccc agcattatgg aggctggaga caaggaggct tgctggagct tgctggctgc 52740 cagtcttgtt aaaatggcaa gctccaggtt tgatgagaga gccctgtctc aagtcaacga 52800 agtggagagc gatggagtag gacactctac atcctcctcc ggcttccccg tgggcatgtg 52860 cacctgtaca cacacatgca tataccacac acacacacac acacacacac acacacacac 52920 acacacacac acacgctaac gtcaatggca cagatctgtt gtaaccttta agctaccaag 52980 ggatctcttg tttatctgaa ggatgaggta atggaagtgg aattgctgaa caataagtag 53040 atgtctacat tctgacatgg attcacacat ccgggtgctt cttgtcttca gtcaggccca 53100 cctgtgctgt aaactgcaag tgacacatca taggtgtgct ataggccacc aggatgcaca 53160 gtgagactct ggaagccttc acagtcagtc acctgcacca agtgctgctc tccacttgat 53220 ctctgcttct ctcgtttaag cttctaatca ttcaataaac acttgtagtg tttttggttg 53280 ttgttgtttt taaccaaata cagtttgaga cagcatgctg atacttgatg catctatcca 53340 ctgttgtagt tgacattcct tttttttttt gattagatgt tttctttatt tagatttcaa 53400 atgctatccc taaagttccc tataccctcc ctccgccctg taggtgacat tcttagatac 53460 atggaatttt cataataatc gcagtttaaa cacagagaga atctaacctt cttatattgc 53520 ttataatctt aagcacaaag tgtaatcttc tctttttttc tttcttttaa atgagagaag 53580 aatatttgaa ctctttagta tattagcttt ttattttgag tatcacgaag aagctaattc 53640 ttagaagcca attatagttc tgttaagaga gagtaatggg gggctggaga gaaggctcag 53700 aggttaagag cacaggctgg ccaggcatgg tggcgcacgc ctttaatccc aggacttggg 53760 aggcagaggc aggtggatct ctgagttcga ggccagcctg gtctacagag tgagttccag 53820 gacagccagg gctacacaga gaaaccctgt tctgcattat agattccagg gaagacacac 53880 agagtataag tgtgtaagta agaaaacgat gacaaccata tagggaaaag aagaaaccga 53940 ggagggcttt acaattgggg atgggaggca gggagagcac ccaggaggaa gtcttgtctc 54000 tctggatgat tttgcttttt ctcagacgtc agggggaagt gagagtttga agccgagtgt 54060 gaaggaacca gattgagcgt gtgagttaga gtgggtggag agagagagag agagagagag 54120 aaagagagag agagagagag agagagagag cgagcactct gctcataaag aaacttatgg 54180 gggagaccag gaagtttcta tgagcagctg gtctctgcaa tgtttgtatt tctttagatg 54240 cccatgctgg gagctggcag atttcaccta taaagggcca agtgataaca gtaagctaac 54300 tgaggtttct gggcagaatg gtctctgtca tagctttgtc gctttcccac agtagcctga 54360 aagcagccac gagtgctagc tacatcagtg tctggactct aggaagcttg tttataagac 54420 gagctggtat gttggcattc ctttgctggc ccctggcttg aaggatggtt tgcttattat 54480 tttcctagtg ttcattaggt agttgagcaa gtgaggaagt atagtctgta cagtagtccc 54540 tggccttgag ctgcttataa gcattaagga aagataagta tatacactta cggtactgcc 54600 aaccaacatt aagagacact atgtaattag acgctggtac aatagacaga aacaggaagt 54660 gcgatgggct agttgctcat ctgcttggca tctgggtgtt tagaaagaga tttaaaggct 54720 acataaggat ggcaggattt gacagaatca caacacgcag gagggtagaa ttggttgaat 54780 attactactg agttcaacct gctcatttcc taagtgagca acccaggatc ctgttgactt 54840 cccaacaggt tgggtaagaa catgccttct cttttcaaaa tctttgtcca gttcttttcc 54900 tgactttttc tgcttgatcc acccagggat gtttatgtac gtatgtttgt gtatatgttt 54960 tatattgtta ggggtgagag aatggggtcg ctccaggtta tcgattgcat ttaggatgtc 55020 aaaagactgg gaccagggtg atcgtgtctg ctacttggac tgatgtggag agaagagaag 55080 ctaggtgagg ggtatgggag ggaagaagca gttgtgcaca atagaaaact ataccctgac 55140 ctatatacat gcaagaaagc agtcaatgaa gtcttggtgg gaagaccctc tgaagtaaga 55200 catacccatc ctgttggttg cgtgcctagc ctttaatggc taagccagct ctgtagaata 55260 tgctcattct gttgaagaac tctgaattgt tagagtgaga cagtttgtat gttaaaacag 55320 acaggtcttg gtgatgtaac agaagttagt aaaagagaaa tcacagagca acatggttgg 55380 ctttttttcc ctttgcatac acaaaaccaa ctcaaacaag agaacgggga agctgagaat 55440 ctgtagttcc aaattgctag gactattaca tagatgttta ttgagggggg gtggaagtaa 55500 aatgctaata tcaagttatt taaaaatgcc aggttaggta gagaaggggg tgactctgac 55560 tgtgagattg ggttgtatcc aaaatgtggc tcaggatcaa agaattttac agtcagaggg 55620 gtcttggttt agtgttaggg actcaagagt tagttagata ccagaaacac tcagattgat 55680 taaaaaggca agaaaaaaaa aacctcacag ccaaacaaaa taagttcctc accctgttgt 55740 atactcacaa cgatctcaga gataagacga ggtacatgaa gaaagtactt tcaaaaatca 55800 tctcagacag tattattttt tctttttcca ttttcatgtg tgtgtgtgtg tgtttgcgtg 55860 tgtctgggct ctcatgtgtg gttccatgtg cacatggagc cccaaggttg acattgggag 55920 ccattctcaa ttgctcttcc ttcctatttg gcgaggcagg tgcttaagcc aaacccagag 55980 ctcagtgata tgtgtcatct ctctagcctg tttcttctag ggatccgctg acacctctgt 56040 gatggaatta caggtagact gatgtatcca cttgatacac actcttcccg ggcaatttca 56100 gttttatact caaaagccgg aaactatgtt agatctctct gacctgtctt agccatgtct 56160 atacacatgg tgggggttgg gagaggaatc aagggtctta attggaagga cccagcctcg 56220 gttttcttga ctctcactat ccatgacctt gtctcccctt tcccatttgt ttgtcttctc 56280 ctctagggtt agctttcact ataatctttt tatattgtgc aggggccatc tttaaatatt 56340 gtcttccatt tggtctattt ctgcccagat ccatggggtt agagggaaga gctaatgtgt 56400 gaggtgcaca tatgtgcatg tgtggatgtg cgtgcgtgcg tgcgtatatg tgtgtgaact 56460 atgtatgtat gtatgtatgt atgtagaggc atgttctcag gtagctcaag tgactctcaa 56520 acttgacgtg tagccaaaga tagccttgaa ctcctgatcc tcctacctca gcttctcaag 56580 tgttgggttt acaaacttgc gccatcacaa ggggatattg ttttgttttg ggacaaagtt 56640 tctttatgta gctcagactg gtctggatcc tgtgatcctc ttgtctgagt ctccctagta 56700 ccggcaagta cttccacaac ttggaagggg aaactcttgt ttagatacag tttttccttc 56760 ttcttcttta aatagcacag tgggattagc tattgactct ggaattggga gagactttag 56820 aggaccctgt agtcttgttt gagtaataag ctctgctctc aacaggcctt ccctctgatt 56880 aggctttgca aagcagagct gctctgtttg agacagaagt gagggggctt ggagcctacc 56940 tcccattgct ccttggcaag aggcagccct gtgatttttg tccagttcag cgcttgcttc 57000 ttgtcccact ggtttcttcc tccatccact tcgctaaaca cagttaattc acactctcct 57060 ttattctgtt tgcaggagat agatgaggta attttatagg tcagcattat agtcccttaa 57120 aaactgataa aggagccagg catggttgtg caaactagcc cagctccgtg gctcatacct 57180 gtaatcccag cagcaactgg agagtctgag tcaggatttc cacgaggcca gcctgggtta 57240 taaaataaga ctctatctca aaacaaaata tcttcaaaat aattttaccc ccacgaaatc 57300 aagaatccca aatggagcaa gcatggtata gagtgtaggg aatgtgcagt tgctgggtgg 57360 ggtttgaaca cagctaagtg accccaacaa gtcagacatc aggaaagaac ctaccatccc 57420 tgtcttagaa caaaagtctg gacccttgtc tagtctggtg ggtgttgtca gcaatgtgca 57480 cactcagggt ggctccctgg atcaggagcc ctgggtgggg aaggctgggg ttgaggctcc 57540 atttcctaga gctttgtgta tctccccagg gtgcacactt ttgagtctac agtgggggtg 57600 gggcactaat cttgtactcg ggctccattg gacagatggt ttcctccacc ctgggcatct 57660 gctgaactcc ggatagggtg tctgggctgg ttgtgcaagt agagggagca ggaaaaggaa 57720 gggccaagcc aaacgctgag agaggagact taccaccaag tagcaggatc ttctctttct 57780 caatttccaa tatgaaatta aatttcccaa caagaaaacc aaccactatc catcgccaac 57840 cacctctgcc cctactttca gactcagaag gaagaaaact aagtatatcg taaactctaa 57900 ggaggaaacc tcaagaaccg gtgagaggcg gggaagtacc ctggccccac ataactgtgg 57960 ggttgggaaa agaatagact ggggtcaagt ttcgagatgc taagaagaca gactcttgat 58020 caccttgcaa gcagtttttt ttctttttct tttgctttgt tctttcagtt accgatgtag 58080 ctgtaaatct gtaaatctag aaggaatttg ttcggtagct ggtagcttgg ggcagccatg 58140 attttcatac aaatttctcc atctcactga ggatggcaaa gactatcagg gccttcattt 58200 tgacagcccc agggaagcag tcactctagt cttttgctcc ctgcttctct ttcacagcat 58260 ctcaggactt ggaacttggg atacccccag gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 58320 gtgtgtgtgt gtgtgtgtgt gtggtttgtc cagacagggt gtctccatgt agccctggct 58380 atcttagaac tcaattggta gaccagacta gctccaaact cagagatccg cctgcttctg 58440 cctcccaagt gctgggatta aaggcatatg ctaccacacc caacgggtcc tccaagtttt 58500 taatctggaa gttggtgaaa ctctgacctg ctgggtgaag tttttatttt agaacatgaa 58560 cactgagtta ttcttgctgg acccaagctc aatgggtaga tttctcaatg gatgcctgca 58620 gggattgtgt ctatcggttg tggccttcag agactggaca aactgcgttt gaatctaaaa 58680 gcaccagctc cattgtttaa tgcacataac aggactgtca aaagtatggc tgtttgttaa 58740 aacaaaacaa aacaaaacaa aacaaaacaa aagtttatca tcaggtatgc aggtgctata 58800 gaaatgacaa ctttggcaca cacctccctc tttctgatgt caagttggtg tggtacccgc 58860 cagatcttga acagtggtga tgacctgtta ttgctgttgc tgttgctgtt gctctgttcg 58920 cctctgctcc tctatctgac acctctctta tttctttcag cttggattcc tcagcaccat 58980 cacagct 58987 <210> 2 <211> 3521 <212> DNA <213> Artificial <220> <223> Synthetic <400> 2 tggtacattc tggtgccaag tgtggccatt cccctggcta tcgccttcct cttcttcttc 60 atctgtgtgt gccgcaataa ccagaagtct tcatcaggta ccgatttaaa tgatccagtg 120 gtcctgcaga ggagagattg ggagaatccc ggtgtgacac agctgaacag actagccgcc 180 caccctccct ttgcttcttg gagaaacagt gaggaagcta ggacagacag accaagccag 240 caactcagat ctttgaacgg ggagtggaga tttgcctggt ttccggcacc agaagcggtg 300 ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac 360 tggcagatgc acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc 420 aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt 480 gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg 540 tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct 600 gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg 660 cgctggagtg acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc 720 cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact 780 cgctttaatg atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag 840 ttgcgtgact acctacgggt aacagtttct ttatggcagg gtgaaacgca ggtcgccagc 900 ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc 960 acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat 1020 cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat 1080 gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg 1140 ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag 1200 cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt 1260 tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg 1320 gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat 1380 ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat 1440 cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc taatcacgac 1500 gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc 1560 ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac 1620 cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag 1680 acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc 1740 gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt cgtctgggac 1800 tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc 1860 ggtgattttg gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc 1920 gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc 1980 cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac 2040 gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct 2100 ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag 2160 agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca 2220 gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg 2280 ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc 2340 gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg 2400 attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg 2460 gataacgaca ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc 2520 tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca 2580 cttgctgatg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta 2640 tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat 2700 gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg 2760 caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc 2820 cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac 2880 gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac 2940 cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa 3000 accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc 3060 catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga attccagctg 3120 agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg 3180 ggggatctaa gctctagata agtaatgatc ataatcagcc atatcacatc tgtagaggtt 3240 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca 3300 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 3360 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 3420 atcaatgtat cttatcatgt ctggatcccc cggctagagt ttaaacacta gaactagtgg 3480 atcccccggg ctcgataact ataacggtcc taaggtagcg a 3521 <210> 3 <211> 2654 <212> DNA <213> Artificial <220> <223> Synthetic <400> 3 ataacttcgt ataatgtatg ctatacgaag ttatatgcat ggcctccgcg ccgggttttg 60 gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc 120 agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac 180 tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct 240 agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc 300 gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg 360 ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat 420 cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg 480 ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc 540 cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc 600 ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg 660 gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct 720 tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga 780 ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca 840 gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt 900 ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg 960 caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg 1020 gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct 1080 taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga 1140 agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta 1200 gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag acgtgttgac 1260 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc 1320 atgggatcgg ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag 1380 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc 1440 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg 1500 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc 1560 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg 1620 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct 1680 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg 1740 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat 1800 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc 1860 atgcccgacg gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg 1920 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc 1980 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct 2040 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat 2100 cgccttcttg acgagttctt ctgaggggat ccgctgtaag tctgcagaaa ttgatgatct 2160 attaaacaat aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag 2220 ggtgagaaca gagtacctac attttgaatg gaaggattgg agctacgggg gtgggggtgg 2280 ggtgggatta gataaatgcc tgctctttac tgaaggctct ttactattgc tttatgataa 2340 tgtttcatag ttggatatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt 2400 cctcccactc atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga 2460 ttcccacttt gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag 2520 aacgagatca gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt 2580 ctaattccat cagacctcga cctgcagccc ctagataact tcgtataatg tatgctatac 2640 gaagttatgc tagc 2654 <210> 4 <211> 106315 <212> DNA <213> Artificial <220> <223> Synthetic <400> 4 cacaggggca ctgcattctg ggaatcaacc tactggcggg gtgatttcat ttcgtttctg 60 acttttgtgt tttggtgtgt atgtatgtgt ttaacagagt gtatggccgg tgagtttggg 120 ttctttcttt ctttctttct ttctttcttt ctttctttct ttctttcttt ctttctttct 180 ttctttcttc cttcctgaaa gtgaatgtat aaagccttta caatgtataa ctgttggaaa 240 atgcccacca ctaaattttt ttttaagttc cacatattct ccatttttgc cttcttatat 300 atatcttcaa cactattctg tgcactttaa aaacttaaca taaacgcagt gtgacttctc 360 ccatatgctg ggtcccgaga ctctcaactt cttaaaaacc taatggcatc ttgtgactcc 420 tagaagtaga cataagtctt tcaaccttca cacctactct ttctgtttta attattattg 480 ctatttgtct tattgtttgt gctttacaag cgttcttgag gacggaggga atctacgacc 540 ctgttgatga ctgtaactct attcgacttt gagttgtctt cttcatgtct tgttatatag 600 ttcatattca tggctgaaac ttgaccatac tccctagcgc tgattgtatg gttttcgtct 660 ggacaccgta cactgcctga taacttgtgc acctcttaac gctactatgc tctgggctgg 720 agaatgaaat ctttaagtca ccaggacttg ctgtttcagt ggcttgacac ctgggccacc 780 aaagaactcg atcttcatct tttagggaca cctctgctgc accttggaaa gccaacctta 840 agtgccagtg gcactttatg cccagctttg ctttgaaaga tatctttctt gtttttttta 900 tccttctctt tctctctttt ttttaaaaat acacatagtc aataggtcca gtctgccctc 960 aaggccttgc tgggttttct tcatcatcca atcactttca ttaaaaatgg ctgcagctgt 1020 aagaactctt gtctgataaa ttttcaacta tgctctcatt tatctacctg ccctctgatg 1080 ctcagtcgtc agactctaat gcaaaggtgg acgtcggctg cctttgcgtg ggcgggctta 1140 gtggtgagga actgatatca gaaaaaaaat gccttcaagt atactaattt attaataaat 1200 attaggtgtt tgttacttgt gtggttttgg tatatttact actgaacttt cgcctttccc 1260 tctgctcagg ttctagccct gttcatctct tggggaagga tgtgtgttct gaaacccttc 1320 tcctgtccaa tctgtaaatc atgaatatta ttagcaaaag tcccttttgg agaggtactt 1380 ggaggttcac tgcacagggc tctgataagc actgccaagc ctttcctagt ctttcggagt 1440 taaaccttag gtaacttgat acctgtagga cgatctgcac acattcacag gcttctagcc 1500 aaaacctcac tgtccctgac gaactccaga gggcttggtc agcagtgagg cggatgtggt 1560 cctgtgttgg tgggatatct caagctcact gcaggttttc ctaagcttgg accccacctc 1620 tgcggaattc caatgatcaa acgctagcaa ctttaacatc ctctttttgt gagaacctgg 1680 aagctgatac tgtgtatact gtgacccccc acgtcaacaa ctgggactgt cttccggttt 1740 cactcatctc tgtgtggcag ctttcaagat gaacatcctt gtttcccttc cctcttgttg 1800 gtgcatgcct ttagtccatt ggaagagaga ggcaggagga tctttgattt tgaagctagc 1860 ctgggagttt caggacagcc tggctacgct aactaaaaaa aaaaaaaaaa aaaaaaaaaa 1920 agatcacaca tgcgcacaca cgaacacaca catacagagc aacaagaaca aacaagaatg 1980 ggtcagttat gatgggggag gaaacaggtt ttgtcaggtt ctcactgtgt cagaagattt 2040 agaactgaga catacaatcc attcattaat tactagtatg ttaagtttat agtttattag 2100 taatttggga attttttatt aataacccaa atttgtggtt ttatatttta caacatataa 2160 agtttaatta ctttattgga tctttaaatt tgtcttaacg ggttcctatt attatgccca 2220 ttttacagat gaaattgggc ttgtaaagct aagtatatga gatggtttct tatcactaga 2280 acagagtccc tgaaaaggaa gaaaagaaat tctttaaggg aggaaacgtt gatcttaact 2340 catggtttcc aaggctttaa gtccatggtt ggctggctct gttgcctttg gttgatggta 2400 aagcagaaac ctggtaagaa aacacgacgg tggaaggttg cttacctcct ttcggccagc 2460 aagcagagag acacaggaag agtcttggga caggataact cttcagtggc acaggtccag 2520 tgaccttcta ctagctagaa ccccctccct accttatagt ctattcagct atgaacacat 2580 ccatggtcga atataccact agctgaagcc caaacctcca atagatgagc ctttgtgggg 2640 gacacttaat atccgaagca taaaaccaag gaatgtgttt aagccctcac tgagaaggtt 2700 agcaaggcta ggtttgaatc cgtgttgtgt atgttttggg aagaagggta gagcgagtgg 2760 ctgtaatcta agactgttca ttctctttat aagatcactg accagaaacc tgttgccacc 2820 tgaaacagtg ggcagaaaca tggcagtgaa atctgtctgg gtcatgagta aagttcaaat 2880 gcaagttaag taagtggtac aaacgtaggc atgagaacta aacattagca ttatatggtg 2940 atcccatgat gcatagcttt cccagaaggc aatgcaccat cagctacttt cttaaagtac 3000 ccagagtcaa gtgtgatgga tggcacacac ctggaaccca gcactcactt gcaggctgga 3060 agaccaaggc cttagggtca gcctggggag agggctcagt cagtaaacca tttgccacac 3120 aagcctgaga acctttggat acctggcaca gcaaagtaca gggctctgtg gtgcacacct 3180 acagtctccg tgttgggcag acagagacag gaagatccct ggggcttgct ggctggccag 3240 tctagctgaa ctggcaagca ccaggctcag gcaagtcact ccatctcaaa aaaataaggt 3300 gtaaaatggt ggaagaaggc atccaatgtc caactgcagc ctctgcatat gtatgcacaa 3360 ggtcatacac acacaaacac agaggaggag gaggacgagg aggaagagaa ggaagaggag 3420 ggattcaacg ttagcctggg ctacatagta agaccctgtc tcaaaacagc catatagcta 3480 ttggagttat agttcattga catttgatca acaacactgc agaaactggg tgtgacattg 3540 catatctccc aattctttca ctccagaggt gaaggcagga aagataaaaa gttcaaaatc 3600 atctttgggt gtatagcaag ttggagacca gcctgggaca cattagatgc tgtaaaaaaa 3660 aacaaaccaa acaactgaaa taaaacaaaa attcagcata aaatactaaa atatgtgact 3720 ttctgattaa agctaatgat agcaccattt gtaccataga aaggtcttct ctggcttgcg 3780 cttcttcctc tgaggagtat agacagctga aaaggaaggc tgctagtgtg agggcagagg 3840 agacaagaga gcaaccagaa gaataagaaa cagaagcaac tgttgggaga tccaagccat 3900 gttatgcagg caggcaggag aaggagtctg ttcaccaatt tgaaggacta agaaggagtt 3960 tgtgacccct gggaaaagac tacagactcg atccaattat aatgaaaaga tttgttgatt 4020 agctgctagc tggatgagta atctctgagc caacttcgag attacttcgc agaagggtta 4080 cggggaagga ctttaaaagg agaatatcac acaaagacct tcaatatcta ggcaagcaag 4140 taacaactgt tctattttga caaattaagt gattgaggcg actcaaaaca atcttgggtt 4200 atctgtgcaa caagttacag aggcaaaaaa aaaaagttag tcataacaag tgttatgtat 4260 ggggaggaag gtgtgactga gtcagggtta ttggaaattt atcttccggg gaatggagtt 4320 agagactagt gggtatggaa atgaatgcct agcataaaac ggagcttctt tcattttctt 4380 cagagagtaa tatagttcct gtgggctgtt ttaggctgaa ctcaattaga tagggctgct 4440 tttcaaggag aaactgcttc aaggagagga aggagctggg cgagcatctg tggttctcca 4500 actttcatgt aaataagtca cccgggagtg agcagcttgt tctctttaaa aaaggctgat 4560 tcccacaatc tgattggaga ggtttgtggt gaaattcaaa aatgaagccc ttgcaggttt 4620 ttggaccaca cttagaaaac tatctttaga gaatttccgt tcctgccaat ggggacaaga 4680 acagctaaga ggagctggtt agactcgcac cttcagaggc ctcctgtcta cccttttaat 4740 gtgcaaatga gatacctgaa ggtcctgctc acaggcagac tctccaggcc tcttctggga 4800 aagggcagct ggcatcctgt gttgctgcca agctcttggg taatacaggc actgctgggt 4860 ttttcttttt ccccgtgaaa ctgggtagtc tgggatgact ttacctttca ggaaaggggc 4920 agaacaaaca caggagtcga tcactcattt cacaggaggg aaggaaacac actttgatga 4980 gctgaagaaa cacccagaac caagcaaatc tggtcaaaac ttacaactgg ttggggctgg 5040 gcttggtaaa agtgcctgca gcagaagcac ggggacagga gttcggatcc tcagcatcca 5100 tgttagaaaa taaggtaggt gcagtgctgc atgccccatg catgctccca gtgctgggga 5160 ggcagggaca gcagaatccc aggggtttgt tggcttgcta gctccagggt ccatgagagt 5220 ctgtgcctaa atgataataa ggtagaaagg tgatcaagaa agacatagaa ctttgggcac 5280 acaaagccta cccctcctcc agcaggattg tataacagca gcagaaagaa aggagactta 5340 cagaaagaaa ggagactcct cgaagcccaa ctatttggca tttgtgcaga gagctctagg 5400 gatgtagctt tcaagggtgg acttcttcac gagcagttgt atttgggtta ctcatacttc 5460 tgtaagtaac tgcaagaaat tcatctggac gcaagaagca tcatttcttt gttccgttgg 5520 cagtgcccta tctgaggcaa atagacattt gctgtgtctc tccaggaaaa gtcacacaat 5580 acacagtgag gctgcctggc agaggaatcc tgtttatctt gtggtcaacc agaataaaag 5640 agacaagaaa ggccagagtc tcagtatacc ctagaagaat atttccccag gtaatcaact 5700 tccctccatt aggctgcacc tcttaaagtg tctgctactt ctaaatagta aactggtcac 5760 aaagattaga cacatgtcct ttgggactca cttctgaact gtacccctgg gctatgaatt 5820 ggtcactttc ctgctgttat gacaaaatat caccactcaa gacaatctaa ggaaaaagaa 5880 tttgagcttc cacttttggt gagagagtcc ataaagacag ggaagagatg gcagctggaa 5940 caggaagcta agcctcaatc aagaagctga gaaagtgagc tgggagtagt gaggctatat 6000 aacatttcaa agtgatctgc ctcctccagc aaggttgtac ctcctcatca aacagtacta 6060 cctacttggt cccatcttgg ttgcttttct actgctgtga agatcatgac caaggcagct 6120 tataaaaaag gaagcattta attgatcaat ttataaacac tttatagggg actggaaagg 6180 tggcttagtg gttaagagta ctggctgttc ctcttctaga ggttctgagt tcaattccca 6240 gcaccccatg gcagctcaca accatgtata atgggatctg ataacttctt ttggcctgaa 6300 ggcattcatg aagacagagc cctcatatac attaaataaa ttttatgaac agcttataaa 6360 aacgttataa aacataattt cagagttacc ctatgactac tatggcagga agcatggtgg 6420 tggcaggcag gcatagccat ggagcagtag ctgagtgctt acatcatgcc tgaaagatgt 6480 tgacagagtg agacagggct tttaaaaaca aaaaccacgt tccctgttat gcacctccta 6540 caacagggcc acacctccta atccttcccc aaacagttcc accaattaga gatcaagaat 6600 tcaaacatgc aaatcatgct cattcaaacc accacagagc ctacaaggct ctcattcaaa 6660 gcaccacaag acagcaagac ctatatgaat cagacctgag gtgatggctg tagcttcctg 6720 agaaagggtt tggggaatct ttccaaggag ccagctttgc tctgtccctt acaagtagct 6780 gtctccccag ctctctagga tcttcctaga gattagatta ggatttctcc caaacaaaca 6840 caaaaaaagt ccaaatggag agggttcaaa ctcaacattt aaaaaaagtg agtcacagta 6900 attatgttgg ggctcaaata tctatttctt aggatagaaa tctctcatat ttaaggtctt 6960 tgggaggaga aacatgaaaa atttctggag ttgtcatgca tacacacata cacatacaca 7020 ctatatatat atatgtgtgt gtgtgtatac acatatatac acacacatat atgtatatac 7080 acacatatgt ctatatgtat atatacatac atatatacac acacatatat gtatatacac 7140 acatatgtct atatgtatat atacatacat atatacacac acatatatgt atatacacac 7200 atatctatat gtatatatac atatatacac atataatatt taaatatgtt ttatatattt 7260 atatattata cataaatatg cttaagtatt atctatttaa atatatattt aatatttaaa 7320 acatatatta taccacacgc agtcctctaa ccctccattg gagatgtgcc tgttccgtgt 7380 tagttagcat tctcctactc agtcaccctc tgcttcatcc actgcataat gtcaaatggg 7440 agatctcaca gagaaagaac atgggacagc aagttcattt tcaagatcac atgacagtta 7500 tttccataag tatagacacg tggatttcac atctcgtttc gtccatttgt ttgaatgcag 7560 catacctgta ggacggtgaa aggcagcctg gttcctggtt gagctaaggc tagaaaccct 7620 actgacccta aagggatgct tggagtttgc ctggttctta ggtaccaggc ttcatgcacg 7680 aggccacagc cctccataga tttgtggccg tcagtaacgt aagggcaaca ccgcaagcca 7740 aagccccccc ccccacctgc cacacacaca caggtagagg acgtgaccca tggtcaccta 7800 gcctcaagac acatctctat tttaatgagg tacctaaagg cctggaggac ttagccaata 7860 aactctcctt cccagatgtt ccttcctgca aaaaaggtat ttaacttcag gccctccctg 7920 agaagtgggg tacggtttta ctcagccacc tttcaccatt acaataaatg ccttaaaatc 7980 atggattgct acttttcacc gggatctgct atggagaaga ccttctttgc ctacagagct 8040 accatctaat ttcttgtaga agacctctct gctctcccag ccatggctgc caccaagctc 8100 aagcttgctg ccacagacag ccagagctcc tcttttttcc ccctcagccc tgggctagat 8160 tcagcccgct ggcccccact ctgttctcag gtcttttgtg gctctcagta tgcctgggtg 8220 tccaagagcc cgagaaccag acagctaggg cctccttgta ggccggggga cccccaagcc 8280 agttctggct gtcctgggat ctcagactgt gctttcccat tctggaaggg ttattccatg 8340 gctccccata gaccaacacc agcccggtga cagtgtgggg taagctctgt caaaactcca 8400 gcgttctgtc tccccaagta gctgtgctct gtggtgggca cccctacaca cacccatcca 8460 cagaaacaca gatgagttct tccacttagt ctgagacatc tgaacagcta ctaggttctg 8520 ggctaggggc tagacgtggg gagaatttga tggagcgttg aaactgctga tgaggaaaga 8580 gaagctctct gttcacggct ctgtcctgga catccagagc aaagtataga aatgtactta 8640 tgctataggc tgaataactg agtgaatgca agctcctgac tgcaagaagt tcccaacata 8700 gcaagagaca cgaagagaga cattcttata acaatggtaa gtgatgagaa atatggaaac 8760 agtaattaat tatatccaag cataatggtt acattccata gaagcatttg aagacccaga 8820 caccaaatac aactcaaaga aaagtgatat aaaattttct agtgtaagcc tagtaatttc 8880 aatgataagt tgcatcaaaa gtcaggaact gatcaatgcg attctatcct cttcagagct 8940 ttgaccacga taagaaatag cttgatgcca gcttaacatt agactctaaa gcagtgacct 9000 tcctagtgct gtgacccttt aaaacagttc ctcgtgttag gctgaccccc agccataaaa 9060 ttacttcgct gctaattcat aactgtagtt ttgctactgt tataaatcat aaggtaaata 9120 ttgatatgca accctccaga ggggtcgtga cccccacggg ttgagaacca gtgctctaaa 9180 gtgaactgca ggtgtgctgt gggctctacc acataaacaa ggatgccata gcttttctac 9240 tgttgttgtt ccccgtttcc aggcttatca ttaaccatca tcaggagata cgaggcccag 9300 gcatgtgaca gcttagcaga agacaggcca agtgcaacct tgtgtggagc tcattctctg 9360 agcaaatgtt caaaggctct gggggtgctc attgctcagt ctcactttga agcctgtact 9420 ataggaacag gcattatttc cactctgttg ataaggaatc tgagacatgc attgtgatgg 9480 ccccagtatt atacagctag caaagggtgg agggagccag gaaggacatt tgtagagctg 9540 agtgactcaa ggtcataaaa tggcaagtgg caagtggcaa gtgaggtgac ttgactcata 9600 cctgcctgga accttttgtc ttgactcaac caaactcctc cttattgatt ttttaaaaaa 9660 ggtatctttt taaaaaatgt atgtgcatgt tgcactggct ggttctgtgt ggcttgagtt 9720 atcatagaga aagatgcctc ccttgaagaa atgcctccat gagatccagc tgtaaggcat 9780 tttctcaatt agtgattaag ggtgggaggg cccactgtgg gtggtgccat ccctgggctg 9840 gtagtcctgg attctattag aaagaaaggc aggggagcaa gccagtaagc agcacccttc 9900 catggcatca gctcctgctt cctgacctgc ttgagttcca gtcctgactt cttttgctga 9960 tgaacagtaa tgtggacatg gaagctgaat aaaccctttc ctccccaact tgcttgttgg 10020 tcctgatgtt tgtgcaggaa tagaaaccct gactaagaca aacgtgtctg tacccgcatg 10080 tgtttatgct taccatgtgc ttgcaggagc ccatagagaa cagaagagag cttctcaagc 10140 gatcctcgga aactatgagc tgtctgtggg tgctgaaaac tgaaccccca tcttccataa 10200 gagcaataag cattcttaac taccagacaa tgcctccagc cctttctttt ttttttttat 10260 tacgtatttt cctcaattac atttccaatg ctatcccaaa agtcccccat tcccctaccc 10320 acccatttcc actttttggc cctggcgttc ccctgtactg gggcatataa agtttgcgtg 10380 tccaatgggc ctctctttcc agtgatggcc gactaggaca tcttttgata catatgcagc 10440 tagagtcaag aactccgggg tactggttag tccataatgt tgttgcacct acagggttgc 10500 agatctcttt agctccttgg atactttctc tagctcctcc attgggggcc ctgtgatcca 10560 tccaatagct gactgtgagc atccacttat gtgtttgcta ggccccggca tagtctcaca 10620 agagacagct ataacagggt cctttccgca aacgcttgct agtgtatgca atggtgtcat 10680 cgtttggagg ctaattatgg gatggatccc tggatatggc agtctctaga tggccgatcc 10740 ttttgtctta gctccaaact ttgtctctgt aactccttcc atgggtgatt gtttccaatt 10800 ctaagaaggg acaaagtgtc cacactttgg tctttgttct tcttcagttt catgtgtttt 10860 gcaaattgta tcttatatct caggtatact aagtttctgg gctaatatcc acttatcagt 10920 gagtacatat catttgagtt cttttgtgat tgtgttacct cactcaggat gatgccctcc 10980 aggtccaacc atttgcctag gaatttcata aattcattcc ttttaatagc tgagtagtac 11040 tccattgtgt aaatgtacca cattttttgt atccattcct ctgttgaggg acatctgggt 11100 tctttccagc ctccagccct ttcttaatca ctggactatc tctccaatcc ttccttattg 11160 acatatgttt aaaatgtaaa gtatttttgt tatgtgtgta tgcaaatgaa tgtgctacaa 11220 aaaggatgtg gacaacattg aagaattggt tctttaccca cccaaggatc aatcccaggc 11280 tgtcattgac tgggcttgct ggccacttag taaaaaaaaa tattacattt ggtaggaagg 11340 ctggctatcc tcggtctaat gaacttcctg acccaaccat cccagtgata tattatagac 11400 atacatcacc atgtctgaca ttgatagttg gattattttt tttaattttg aaataagtta 11460 taacacatta agtttaacaa tactataacg ttcacatttt cttttccacc agtttccccc 11520 aagtaagtta gccttagaca taatcaaaac caggagattg acattattat attaccaagc 11580 aaagacggat gctaggtggt tcacgctggt ttcttgcaca cacttttctg atgtatttta 11640 aacatcagat gtactgattc ctgtacccac cactaccatc tggaaatagc actacgaaat 11700 gtccctaagg acactcttgt tcacaattgt accttccctt tcaccaccag acatggcaac 11760 tcttccacac agttgtcact tacagagtct tacatcaatg aaatctgggg tgaatgtgcc 11820 atccaaagaa acacaggaac cagagttggg ttccaggaac ccatgagaaa aagcttgggt 11880 agtagcatac actggtagtt ccagccctgg gaagacagaa acggtgaacc actgagattt 11940 gtagcaagcc aacgctacct tttctcacat ttagccctac ttggtgagcc caggccaatg 12000 agagaatctg tctcaagaag caaggtgcgc gacatgtgag gaagaatact caaggtctcc 12060 tctggtctcc acatacacac atgtacatac ttatgtacac ccacacataa atttgctcct 12120 ccctttggac acatgtccag gagtgtttag aaactggatc acataccatg taacctccag 12180 agactggctt ttttctcatt gatgtgaatg tagcatagtt taggcatcct cacgctgaag 12240 ggcttttgga tcatttttaa ttgaagctat ttacaaataa cactgcaatg accacttaag 12300 aacagtatcg acatggattt ttatttcttt gacaaatatc caggagcagg atcgctggga 12360 gtattagtta cttttataac acaccgcaac aaaatcagct tttgggaggg tttatatggg 12420 gcttataaag gaatgagtcc agcaccatca tggtggggaa gcatgtctgg aggcaggaat 12480 ggtggctaaa cagcagctga gagctcatgt cttgaacaat aaacaagaag cagagaggct 12540 aaatttgaaa tggcacaaat cttgaggtct taaagcccat ccctagtgat gtattcctcc 12600 agcaaggtca cacctactaa gcctccccat acacccatca gttagggacc aagtatttaa 12660 ctgcccagac catgggggat atctcattca aaccactaca ctggataaag gtaagcttaa 12720 tttaaatgag aaatgccccc gacaggctca ggcattgcac acttggtctc caattgtggg 12780 cactatttgg ggaggtttgg gaggtatggt cggtcctgcc ataggaagta tgtcactggg 12840 ggagggcttc gagaatatat aaccccgctc cacttaccat ttgctccctc tctactgctt 12900 gtttgtggtt ggagatgcag tcagtctctt ggttccctgc tcctgccacc atgcctgtcc 12960 cttgcagacg gactcttctc cttccagacc caaaagctaa aaataaactc ttaattccat 13020 aagttgttct tgtcatgata ttttgtcaca acaacaggac aattacaccc ctctctcccc 13080 aaggttatat aaatagtaag gacctctgtg gagacaggat tatttccagt ctgttgtgtt 13140 gtctgcaaat catgtagaga gctcttggga tacacccttc atgagtcatc acctatactc 13200 agggtttttg agtaatgcag tattgtactt gtattgtcca gtgttcctgt cagtaagttc 13260 tcacccataa ttctgtaagt aatccagata aacacattgg cttttgaatg gacactttgg 13320 taggatagtt tctttagtct gtcattggtg ctttatctgg gctgagtaga tgtttgcatg 13380 aagtctacac tctttgtttt ttgtttgttt gtttgtttgt ttcaagacaa ggtttctatg 13440 tatagccctg gctgttctgg aactcacttt atagaccagg ctggcctcga actcagaaat 13500 ccacttgcct ctgcctcccg agtgttggga ttaaaggtgt acgaacacca cgcctggctg 13560 aagtctacac tcttataaaa aaaatagtcc tgttctcact tcatttctgt tgctgtgata 13620 aaacaccttg gccaaacaga acttggggaa gggaaggggt tcattatggc ttacagttcc 13680 aggttctgga cagtcgatga gaaggcaagc ttagtcacct cacacgcata gtaaaggaca 13740 gagaaaaatg aatggatgcc tgctgcctgc tgcctgcttg ctgactgcct gcttctgttt 13800 atcccctgcc ataaaatagt tgagaacctt ctgcatagag aatgttgctg cccacactgg 13860 gctgggtcct cctacatcaa tcaacaatca agacagtctc ccacagacat actcacagga 13920 tctaggcaat ttctcaaccg agactctctt ctcaggtgat tctgggttgt gacaagttga 13980 cagttaaggc taaccatcac agtgtatatt agaacttatt tgttctaata aacttagtaa 14040 ttcccatggg tcattagctc tacctggtag gaactatctt tgtacacttt gttaatacaa 14100 attgactact tgttttaata cctcatataa aatgacagct gtggcatttg tctttctatg 14160 atagagttat ttcacttagc atgtctttaa agtttatctg tgttctcatg tattacagaa 14220 atattcataa tgttaaggct gaataatttt catgtgcatg tgtacagaca catgcacata 14280 ctttctttgc tcatttgtta tgagggatca tacaggttgt ttccttaagt tggctactgt 14340 gaatagagct accatgaaga tggggtgaaa atatctgttt gtgatattta gaatggctct 14400 tttgggtaaa attcttttga atactagatc atgtaagagt ccaattttta attttgaaaa 14460 aatatttatt tttattttgt gtgtatgggt gctttgctag catgcatgta taccacacgt 14520 gtacagtacc tacctgcaga ggtcagaaga gggtgttaga tcccctagaa ctggtgttac 14580 aggctgttac gagctgcttt atggtgctgg caaccaaacg taggtcctct agaagttcag 14640 acagtgctct tagccactga gccatcatct tctggatgta tttttgagaa cctactatac 14700 tcttctgaca tggcatatca ttttacattc tgtcagttgt tcaaaaagct tggagtttcc 14760 ccatatactc attaatactg gtttatcacc atcatcatca tcatcaacaa caacaacaac 14820 aacaacttta ttttataatg gtaattgtga cagatgtaaa gcaatatttc atgttggttt 14880 tgatttgcat ctttcagatg tcagtgacta tatcctcgtg ggtcactcat atgacttcac 14940 tgtagaaata ctttaattat ttaaccccct attcctcccc tctccacttt ttatagtaat 15000 gagggttgaa cccagggcct catacatgcc aggcaagaac attacccctg agttgtatca 15060 gtcagcccta ctcaattttt cagcctcttt taacatcaag atagtaaatg tgttgctgtt 15120 tggaaagttt ggggatttta ttacatcttg ggagttcctt atatactttg ataattaacc 15180 ccttagtggc tggatggttt gtaatatttt tatccttttt cctgagttgt ctcttttttc 15240 tgttaatgtt ttctttcctt atgtgatgct atattggtct gaatgagaag tgttccctgc 15300 aggcctgagc atttgaatgc ttggttcctg tcttagtcag ggtttctatt cctgcacaaa 15360 caccaagaag caagttgggg aggaaagggt ttattcagct tacacttcca cactgctgtt 15420 caccaaagga agtcaggact ggaattcaag caggtcagga agcaggagct tatgcagagg 15480 ccatggaggc atgttactta ctggcttgct tctcctggct tgctcagcct gctctcttat 15540 agaacccaag actaccagcc cagagatggt tccacccaca aggggctttt ccgccttgat 15600 cactaattga gaaaatgccc cacagctgga tctcatggag gcacttcccc gattgaagct 15660 cctttctctg tgataactcc agcctgtgtc aagttgacac acaaaaccag ccagtacagt 15720 tcccagttgg tggtgttttg gggggaggtt tggataccac aagcttgttg gagaaaatgt 15780 atcactggga gtaaactctg agaacagata gtttcccaca tgtctagttc atacctgctg 15840 ctttgtgcct gtggtccgag acatggctct cagatctctg ctcctttcac catgcccgat 15900 tgctgccgta cctcacacca tcatggacac ttatccctct ggaaatcatg aggcaaataa 15960 aatcttcttc ctataaatcg ccttgtactc ggtattttat cacagcaata gaaacggaac 16020 tatgtagatg gggtccctgt tgtctacttt tgcgtctgtt gcctgtgcat ttggtgatgt 16080 atctatgaaa tcgtagccaa gaataaaggc aaagctcttt ccccatctgt tttcttctga 16140 agttttatca tctggggcat atctttaagt ctctaattca tttagagttt gcttctgggc 16200 ataactcttt ttctcaatat ggcagtgttg gggactgaac ccacagtctt gtgcatccct 16260 ggtgagtact acttcattta atgtttagag aagccccgac tattccctaa cctgactctc 16320 cttctcagtc aagtttttat tgatggacgc agctccagtt tgtatcagag aatgaaggtt 16380 agcacagtat ctgttttcat ggaacttctg gtaaaatata catccatagg ccaatataca 16440 tgaaaaacaa ctacacccct gactgccttc accaacctat tgctggtatt ttatttgtga 16500 tagttggagg tagggtgtgt gcctgccact gcatatgcgt agaggccaaa ggacagcttt 16560 gtgagtagtt ctctcttacc tttcttagct tctaggaagt gagctaaggt ctgagggctt 16620 gtgtacaagt attttatttg ctgacgcacc tcaccaccct tcattaccaa catttcaatg 16680 ttccccagct gtatattaaa gggcccaaga agtctataat gcagtaacct gcttaacatc 16740 actgacacag aacttattaa atttgcatga caatgtaaaa catacggatt agtatataat 16800 ttaatttaaa gaaatcttaa tttgcttgct tttataaatg caatttgtta aattgcctca 16860 tggaacagct attttcttca gaggcaaata aaagagacac taatattggt aatcctgctt 16920 cacccaggaa gagcactggt cctagtcagg agtccagcac cgccgtcact gttgggtatg 16980 tctttacatt taagtcaagt tcagagttca gagtaagaga ctatcccaga tgctagggtg 17040 gctttgaggg ttctgagaac ccaaaggtca cgtgaaagat atggttacta ttgtcaggct 17100 gattctaatc agaaatcaga gtctggagat ggagcagata ccattgagaa gcagttagga 17160 ggtggtaatg tggcactgac aacccagcca aggctcaggt agcggtggac atgcgcattt 17220 gctttctctt gtgtttatcg ggcaacaagc ctgggtgaac tggaaagcgg atgcggtctg 17280 aaggtctgca tgaaaccaca gatgtcgatc cgtggggggc agactccggg ggcatgtgtc 17340 tcaggggtat agcacttgga ggtggcaagg actaatttca aggtctcaca ctttaaaagc 17400 ctaaagtcca agcataggtg ggagcccagc ctagactcag agcgcagacg cggacagaga 17460 gcatgctagg agcctctgtt ggacagtcaa acatgaccga gtaagaccga aactccatag 17520 agacagactg agggaaggga gaggttaaag acagggaacc tgggctctaa gatctccctg 17580 taacagctct ccctttgtaa gattggatcg aatccctagg gtaaggggca cagctgagct 17640 tccaggtgag acaagagtag ggagaagaac catcaggtaa gacaatatag ttagagcccc 17700 aaaccaatga accaactaac caaccaacca accaaacaaa caaataaccc aaagaccaaa 17760 aaaaaacccc taaaaatcaa aacccaaacc aaaaaacctc aaaacacaaa acaaaacaaa 17820 aagacccatg agccacagga ggaacacagt tctgtgaacc ctgggctcaa acaagggtat 17880 ggatagaaat taaatgttat atggatggaa ggggacctgg agaaatccca gtgttttctc 17940 ttggatgaag actcttgatc cctgaaacta agtctcacaa gcccagatga agttgtgagt 18000 ttaaatcttg tcaagtgtga aactagagcc aacagtcaaa tgcctccctc caccagctaa 18060 accagtccat tttaacctgt ggattaaatt gtggtttaat tttaggccag aaaaaaattt 18120 ttacttgcca taaatttaag tgctttagat acccatgaca taaagactgg agcattatgt 18180 aaaagggagg cacattagag gtcatttaat gacttgtgcc acttcagagc tttctatcac 18240 atagtccaca gcaattcaaa gtcatgaccc ctagaactaa aaacattacc catcagcctt 18300 tgaactctga aaccggccaa gtgtaataag cccagagcaa agtacagact ttccagtgac 18360 tgagcacagg cagtgactag accagatcgg ccatagtgag accacacact ggccctactc 18420 agcgggcatt cactcagcct agattccatt ttggttgctg ctcacctagt ctcaaaaagt 18480 ctcagctaag tgatgtgaca gccctttgtg ctaggacatg atctgttcta aatatctttc 18540 cacaggtgtg ctgcaaatca ccgtcaacct gataagaaca atccctacaa taaagtgagt 18600 tatttatacc aacatcctcc catgttgttg aaccaatgaa gcaaatgtgg aagaataatg 18660 tttggctcct ttctgttttg aaaacatttt ctgggaaagc aggtgtttgc agactaaata 18720 gatatgcatg ccatgggtga tagatagtct tagatgtcag gtcagttctt catcggggac 18780 agatctttgg aggacactgt aatattggtc atgacagaat agattctgat agaagttggt 18840 gtctaaaatg aaaatggctt ggagaccaca ggtgatcggg gagttcctca tggctgttac 18900 ttataggaag aattcttccc tcctataagc cctgctccat ccagctccca aagatcaacc 18960 agtattaaga ctgacgttgt ttaaagcacc actatctgtc cctgtatcta ctccaacgca 19020 ttgagccagc tatatagcac tttttttccg ttccccaata accctaaatt tcttctactt 19080 cttgttggag atgtttcctt tgccccagat gtctttcatc catccactaa gtacttccat 19140 cccttcctgt actagctaat tctttcctcc cctccaagac tcatcacacc accgtgccct 19200 ccaggatggc ttctctcagt cctcactcat ggactgagag gtgctcccac ctcacttgcc 19260 tggtaggtgc tcccacctca cctctgcatg ccaggagcta agaaaaggaa gttagaacat 19320 tactagagat tcgcttggaa ggaaggcagg aactgagatg gaaagatggg ggggggggga 19380 atgataaacc atatggagcc acatgaaacc tggggttgtg tgcagtaagg ggtgaaaagg 19440 gatctggcta actgggtgtt ctcaacgcaa ggaaagttca taagacaaac ccaaatgaga 19500 ccattaatac tgccaggtac agggcaatgt ctacaacaac gacgatacag aataacccgt 19560 ggcctggaga ggtggctggg tacatagagt gtttgctaag caaacaggag gactggaaac 19620 cagaacttta ggatcatata cattccagat gagcctggca gcccaccaat aataccaata 19680 ctaattaggc agaggcaagg ggagctccag ggcaaggtgg cttgctgaac tagcaaaatc 19740 agcgtgctcc agattcagct ggaaaccctg tttcagtgaa taagatggag gcaataagac 19800 atccagtgcc aatctctctt tcccacatcc acatttgctt ggtcacacag gagtatccac 19860 atccatccac atgcatgcat acacagcccc acacgcatac acatgtggga gaaaaggaaa 19920 aaggaaagaa taaagtaggt agacatgacg tcgagaagca atttaagcag ggtggatgtg 19980 ttttctgtcc atcccttgtg tgtttgtttc tctttcctaa gctccactgt tcattagggg 20040 aaagttcaac tcacataaac ttttcatgtc ttcttcagat atcctcacct tgagatgatg 20100 tagtgagtag ggtcttgtgc acaaatccct cagggtttgg agtggggtat gggtgtcaaa 20160 gggcacaggt gaaacaaacc tggctgtgca ctgattcttg tagggtctag ggttttatgg 20220 ggtgcttcta cttttggggg gacttgacaa ttattcacaa caaaactaca aaacagcgca 20280 tcaggaacta tgggttcttc tcaagtggag ggcattacat attaactgaa tgcgtacaca 20340 tgcctttaag atgggatatg catatgcact gtctaagata ccccgagaag gcactgggta 20400 tttcagatgc tgaacacaga ttttcctaag ctcttgcctt caggccaaat tctgcttttc 20460 ctggactatg gcactgtgca gagaaaaatt atttgagggc aaggtggtga gaagtcttaa 20520 aggccccata gccgaggatg gggtagggct gtctcttctc ctgtgatcac tgaaggcctc 20580 ttatattcaa cagacaccaa atctgagagg tcctaccatg gccaaggcca tatccccttt 20640 cttatgttca gagatggaaa aggaggataa tcaaagtgct taactcacag ggtcattagt 20700 accatgaatg tgttaaggtt tagtccagtg agttgttagc atcgggcatc tgtgctttac 20760 tatcagagtt agtgtttgcg ttggagttag tgttatgggt caatagttac cagttagtca 20820 gacacacact atgatggata ggttgtttat agacagagag aagacatggt tttctgaata 20880 taagacgggg aaggagtacc agggctagga atgagcagag aaactatttg aggatgcagc 20940 tgtgtataat ttaggcaggg tgtgtagggg aggggagaga tgtgaaccag gaagcaaggc 21000 agggctggat ggatgatggt gggcctggca tggggcagca aagttgagac ttgaagcagg 21060 aggccccgag gagccaggga gtgacctggt ccagggaggg gcgagatgaa aacatacttc 21120 aggaggatta ggctggcatg agatataaag tgtctcggtt ttgggcaggt tcagaccaag 21180 ggtcgagaag tccctacaga gctggaaatt tctagacttc ctccagcctc aggctccaag 21240 ctacattttt gccaggctga gtgggaatcc tggagttttc catgggaccc aacaattcat 21300 ctttgtgtct tctgttaccc tcagctttgt ttttgtgttg cgtaacattt ccaggtctca 21360 atatcccaag caagctggag ttttcagagg ttaaactcta gaacctaccc agcctgggtg 21420 gcatctctgg aagcaaaaag agacaccccc cttttgacca gagatccttg tagacaaatg 21480 gccttagctg accctggctg ttctttggtt catataacaa tactcctctc ctctctctag 21540 tccttctccc aacctgttta gacacaagac ttacataaac cttctccact cccctctgct 21600 tcttcaggtg gaaccttgaa gaaaaaaaaa gttgttacta ggccaaggag caaggtttgg 21660 aaagacacga tgtgctgcca aatccaggaa agcaagttaa tgagaaggtg agactctgtc 21720 cacccacttt ctttcattgg cggaattttt aggtaacaaa catagctcca ggcaggtgtc 21780 tgtgccccac tcccatgctg agcaggagct ggacccgagc tgcaggtccc actcagccat 21840 tcagtggctt catctctgca ccatctccag acccctatgt ccaattgcac agcagtgatc 21900 caggtccttc cctctcgaag tccttttgag ctctagcttt ccaggatgcc cttctacggg 21960 tcagtgtgtt ggtctggatg agtcctgggt ctgaccagaa ctactctgaa agggaagata 22020 aagaaagagc cttagaagaa ctagctgtcc taacaacagg agaccccgta ctcacatact 22080 aatgtgcact catgagctca tgtggaagta tacatataag caacactggt gagcacatgc 22140 acacacacac acacacacac acacacacac acacacacac acacacagca tgcatgcatg 22200 cacgtaaaat aaagcttata aaacaaaaca aaacaacacc aagaagcaag caagcaagca 22260 agacctaggg tttttataca tctgttgtgg gattgttgtt gttggtctta ttgtgttttg 22320 catttttggt ttgagacagg ttctcactat ggaaccctgg ctgacctggg gactgcctgc 22380 ctatgcctcc cagagtgctg ggatcaaagg tgtgtgccac ccatgcccag ctgctgtgga 22440 ttttataggt cgttgatggc tgggttttgg ttatgagttt aacactgctg ttgaatgcag 22500 agagcctttt aaatcgagca tcaatgaggg gttgttttgt tactgagggg ctggacatgg 22560 gtttgcttag gactgagatt tgctttctgt tgcctccctt gttctgagcg caattgctta 22620 gctagatgtc gatggccttt caccatgggt ttcctgtgag cgttacaagc tgagattggc 22680 aggtgcattg aggaggtggg caaggggagg ggcgacagca caatgctgtt tccctattgg 22740 tgctaagtgc tgttcctgtt taataagtgc agttttgaac tggaagagag gaggtgtgaa 22800 aacaccacac gagtccccta aaatagcctc ttaacaacca aatcaggagt agaatctggt 22860 ctccgttcag gaaacagggt gcttggatgt ccactcccct ggggctcctt agcccgtctt 22920 tccccttagg ttcaaaggtt cttcataacc tttgggtcca ttgactttac ttctgataat 22980 atcaatgacc tggggacatc caccttacat atgaaggacc cctgtcggca tgagtagttg 23040 tctattaact gtcaccatgt agtcagcttc tctggcagag atgattacaa tctaagtcta 23100 gaatgctggg accctttaat acagtttctc atattgtggt gacccctaat cttttttttt 23160 tttttccgag acagggtttc tctgtatagc cctggctgtc ctggaactca ctttgtagac 23220 caggctggcc tcgaactcag aaatctgcct gcctctgcct ctttgttgtt acttcatacc 23280 tgtaattgtg ctactgttat gaatcataat gtaaatatgt gtgtttttga tagtcttagc 23340 ccctatgaaa gggttccttg aaccccccaa aggggtcgtg ccccacaggt tgagaaccag 23400 ttctctaaag agccatttat gaccccctct ttggctatca ggcttctaaa agaatttccc 23460 taggaatctc cacatgggag aagaaatagc caaaacccca gggacatcca gaggaagacc 23520 taatgttttg tgaagaattt tgcaacttat tagatgactt catagagagt tggcgctgag 23580 acggccgtgg gcagaggcca caccttgtct gaactattca actatgcata tcttttcttt 23640 ttttatgtac cctcacgtat ggaatttgaa atcaaacttg gatgtagtgg tcactgtatt 23700 tcctttgtcc ctctttccat tgtggccact ttgaataaat ctccatttta atttggctct 23760 tttatttggc ttgttcagga taggtggcca aacctggcta gttgggggct caggttgaaa 23820 ccctaatctg gtaatagtaa catatgtcct gagtctggac ctgtacagcc atgctcagta 23880 atgaagaaca agcagcatat tggatctctc ctaaggggtg aatttcaccc ctgctaaata 23940 agcaataatt gatcaaacaa ggactggctg catgcttttt atgctcctca gtattcgggg 24000 gcagcctcct ctttttcact actccaaatt ctcattctga cttgagctta gagttgtgtt 24060 tactccagac tcacgcggtt tcccttgatt attattgtgt gcctctccct ctgaagctga 24120 agaaatagct gatccttacg tgcttgtgga gtaagcaaag aagtcacatg actggatcag 24180 tacaaaaatg tattccgctt tgctgtagtc cagtgaattt gaagccatgg atattttccc 24240 ttttaaatgt acagttcatg tttagagaaa ggaacgcaac gatgtatcaa ttgcttgtat 24300 acttactgtc aaagtagcag aagttaacat ttaggctcag tacttttgaa tttccttctt 24360 tcttgaagaa atggagcatt acattcattt ccatctctac tgagaaaacc agggctgagt 24420 ggccagccat gtgcttgctg tctaaaatgc actgatgggg actggagaga tggctccatg 24480 gtaaagtgtt tgttctgcaa gtatgagatc ttctgtctag acatccagaa caacatctaa 24540 aaatatctag atgtgatggt gccttcctgt aaccccagca ggggtaggaa gggaacaagt 24600 ggatccttgg ggctgtctga tcagccattt caaagaatca gtgagatcca tcatgaatga 24660 gagaacctgc ctcaggaagt aaagggagag gctggagcaa tagctcatgg ttaaaagcac 24720 tggctggttc cagctctagc agcaatctac acggtggttc acaagcctct gtaaccctag 24780 acctaggatg taatgtgccc ttccagtctc caaggatact gcatgcatgc atggtgcaca 24840 gacacacatg caagcaaata cccaggcaca aattaataat aatgatgatg atgatgatga 24900 tgtagataga tagatgatag atgatagata tgtgataaat agataatata gatgacagag 24960 ataaatagat aataggtgat agatagatac ctagatggat agatagagat aatatagtta 25020 gatagatata gctagatata gtatagatag ataaatagat agagatagat aaatagatag 25080 agatagataa atagatatag tattgttcaa tagagataga taatagatag atagatagat 25140 agatagatag atagatagaa tagtatagta tagtatagta tagtatagta tagtatagta 25200 tagtatagta tagctagcta gctagatatg agtgtggtag cacatctggg aggcagaaaa 25260 ggtctgcaag tttgaaggca gcctggatta caaagcaaat tccaaggcta catagtgaga 25320 cattatcttt taaaagtaca tatataaagt aaagcagaag cagaaataag gaaggcactg 25380 cccgttggct tttgtccaca tacacgtaca cactcttact cacatgtgca cacatataac 25440 cagggccata cagaatacac acacacaaag tctaagaatc tagggccaac gagatgtctc 25500 actgggtaag ggcacttgct gctgagcttg atgacctgag gttaagcctt gagaccagct 25560 ctcacagatt ttcctctcat ctctacgtgc atgcaagtgt gtgtgcgcgc acgcaggagt 25620 gtgcatgaga ccagtagagg ggtctttttg tccattgtca tcactgtctt caaatcagcc 25680 atatcaggca cttggaactt gtccctttca tatacttcct tagaaatccc tccgttcaat 25740 gtcacttttc tcatttggac tggttaaaca attccctgga ctgttctcta gaaagacaga 25800 tgtgtgaata attaactcaa cctcattaat cagttgctac cccggtttct aagcttcaaa 25860 gctgagttac ttgcccttag agcatagaat tggtttataa atggtgtatg cattcatgta 25920 tgctaaagga aattatacgt tttatagaac tgagaatatg taaagtatat aaaatgtact 25980 gtgaaaaaaa ttaagaagat agagacgact ttttaagcaa attcaaagag cttaaagtct 26040 tattgtagat agtgttttaa atttttttca cttataatta ttgtgtttgt gtgtgactgt 26100 gtgtgtgtgc atgtgtgtat ctgttcatgt gcatgttcca cagtgtgcat atggaggtca 26160 gagggcaaac tttcagagtt cacgttccac tgtgtactca gaggctcaaa ttcaggtagt 26220 caggcgtgca cagcaagcat ctttctacac cgagggatct tgcaagccca agtgacatca 26280 tatcgatact cttaaaagtc attgccttca agggcaaagg tgatgtcata aaaaagctcg 26340 tgcttaagtc agcttaagtc agagagcagc ctgcagagtt ctgtgctgct gacaccgtgc 26400 tcattgcact aagcctggaa ctatgggaag ctcacagata accaatacac agacagttcc 26460 ctgaagactg tagtcaagga gacgggtgca cacagctaaa cagaccacaa gccagcgaag 26520 cagatgctgt gacagtcact taaaggacat gctgtgggtg agactaggga gctgggagta 26580 ggtgccaata cagcaaaatt ccttgtgctt accaaatggt ccatcctcac tgtcagatgg 26640 caggggcatg caggacacag tggtggtctt tgacttggtt atgaaggatg aaaagacttc 26700 aacaggaaga caaattgggg atgaagatgt aggcagtgga gggaaaggag aagcagtcta 26760 actggggctg aagagataac cagaggacac agtgttggat attttcactg agattaatca 26820 acagttcagt ctttggtcca tcagcccctc ctccacacac acatccccca ctcgtttttt 26880 tgaggtatgg ttcacgtgtt ccaggctagc ctcaaactca ccaagtggtt agctgaagat 26940 gaccttgaac tttagactct cctgcctgta cttccccagg gctgggatga caagtgtgca 27000 ctgccctgcc tcaggtgtgc actgccacgc ctggcttgtg ctgttactag agatggaacc 27060 caaggcttct ggtgttctgg gcaagcacag cctggctcac atccccagcc ctggtccact 27120 cttgtctctt tccacagtga tctatctagt cttggtccca gcaccttgct cagagggagt 27180 ggtcaccgat gacctctccg ctgtttacct ggatggaccc tccttagtcc ctcattcctt 27240 ggcctcttgc tgatgtcact tgtcacgtca accttgatgt cgatggccac actgacattt 27300 gggtcccttg tggtcttcat ctgtaacatc ctgtccaccg ctgtattctc tttgttttgt 27360 tttgttttgt tttgtatttt cgagacaggg tttctctgtg tagccctgcc tgtcctggaa 27420 ctcactctgt agaccaggct ggcctcgaac tcagaaatcc gcctgcctct gcctcccaag 27480 tgctgggatt aaagacgtgc gccaccactc acggcaatat tctcttttat ctatgttcag 27540 atgccttttc tctgtcagtt cttcctcatc ttacatcttt atgagcatgt ctcctgcagt 27600 cttaaggaca tgccctctcc tacaccatgc ctgaattgtt catatctaac tttcaggtct 27660 catcttaatt attactcacc ttaggaacag tttccccaaa ctttgcatct gtccagtaat 27720 gagcctagag ataaaacttt atcagagata aatgcctgag tacccagctg tcttcctaga 27780 ttagcccatg ccattcttta gtttcttttt ttgcataacc cgattataaa tgtaaaagtc 27840 atgtttacca taatattccc agcacttaac acaggctaat caatatcaag ttcttaataa 27900 atattcaatg agtaaatatg ttattcaggc aacatcaaga atgctgggtt gaataagtag 27960 ggttatatag gaagaaacaa gagaagaaat aggctggatg aagaacttta gctttccact 28020 tcaaacattt aaggctattc tacagataaa actgctattt aaagttgtgg tccagtcttc 28080 ctctagtaac atgctttgtt agctggtgta gtgtaaccaa ttagccagtt tagtcaaggg 28140 ttagcagggt agactacacc acaactgtaa gagcagcaag acagaaggac tgaacaggga 28200 tggtgcccaa gagaatgctg ggagttgata gcgaggcaag gccccattcg gcatcctttt 28260 ctgccctgtt atcattcact aggagaaaag agtgcctaag gaaagccaga cttagaagga 28320 agagaggcag agagctcatt tcctgatgca gaatcagccg gcaggatatt agtctatagc 28380 tcaggggctc cctgagcagg agctgtggat ttgagagata tttgggaaga actattagat 28440 gaagatgtga gggttgtcaa ggaaagcaga caaagggaag cgagtcaaga gctaggcatg 28500 tgtatgaact caaatagcaa caacaacaac aacagcaaca aatgccatgt atacatggaa 28560 gagctcgtgg ccgaaatgac aaaggaaaca aatgttttaa actaaattaa agaaatgatc 28620 acgtttagat attgtgctta aaatcttcag gaggtgaaag gtgatctgga agctgacacc 28680 gagcaggggc aaaaagagag catgtcacag ttaggtttag tgggtagaag gtaacatggg 28740 gatgatcaga gaaccactga agtgggggag gggtggtgat atctttgttc ttcagacaca 28800 gaagagcaga aacaaggact caggcatgcg tcgtgacagg gttagaggct tggttaggta 28860 gaaagttagg tttccatgtg tccggggagc ggggaggaga agaatgtgtt cagggcaaag 28920 atccaatgta taaactttta tggccaacag aatgagggct ctcagctaca gaggaaacac 28980 ttaatccgcc tgtaaatttc agtccgtcat actaacctga catggcagct ggcgtcatcg 29040 ggacatgagc aatggttcca agcagagttg attgtgcttc ctcactctaa ttattaacca 29100 gtcactgtgt tagctcattc acgttgagaa gcctcccgcc tcactaccca tctgtatgga 29160 gacttctttt ctacgaaaga agtatgctca ttccaaataa catcaagtgt ccaaatcatt 29220 agtttttact ctttgttttc atatgctatg ggttaagtgc agagctatta aaatacaaaa 29280 aaaaaaaaaa aaaaaatctg gaatgagccc tgacaactga cttgattcca atcttatctg 29340 ccattttctg gctacatcat tgggagactt acttaacctc tcttaggtca aactacatta 29400 ttggatgttt cagcccattc taattttttt tcttttctca gccttttcaa tgtgtattta 29460 ctacttgaac gtttttcatt taaaaaacaa acaaaaaaaa ttgaaaaaac tcccagaaca 29520 tctgctatgt gtcagctact gaaggaacac aatgtttaag acctaggttc ctctcatgag 29580 tcctaagctt gctttagtag gcagtgacag aaggacaaac tggtccatcc atctagataa 29640 acttcggaaa gtgaccgtgt ccatgggaag tcctccttag acactgtgac ccttgagttc 29700 agcctcagag aatggatcca agttaattgt aggtgtggaa ggttgtgttt tgacacaggg 29760 gacaggcaag tgacaagaag gcactgagtt caagggcaca gtatattcta agtgtcagtt 29820 gccagtggct gaacatagag gagggaaaga ggttagagaa gccaagttca aagctcagac 29880 tatgcataca gtataaggtt aaggcactga tcctatagct atgttaccta tgaatgagtg 29940 gtcgttcttg ggcaagggac ataactcaat aggtttttcg cttttccagc tgtaaagtgg 30000 aattgaaaat ggtatggcat agcgacctca catagaagta ttcaaaacaa tgtctagggc 30060 acggttatgg ttatggaagc ctcttgaagg gctccagcaa gtagagattt gagttgcagg 30120 cagaggcaag cgaagagtgg attggagagc cgtggaaacg ggagtggaaa gatgttagaa 30180 aaaaaataca gcctgcactc aggagagggt atggagatac aggtgaagca gacggctggt 30240 gtcagtcttg acagcccatc aggtgaagat aggatgagcg gtaactaata cccagagttc 30300 tacttcgaac acatagtttt ctgtttgtac cctaactaca tgcacgcgcg cgcacgcatg 30360 cgcgcacgcg cacacacaca cacacacaca cacacacaca cagatacata cacagacaga 30420 cagacacaca taaacagaca cacacacaca cacacacaca gagagagaga gagagagaga 30480 gagagagaga aatgactata tcataggctt tggggcaggg acaggtgggc ctaggtacag 30540 agtcctaact tttatcttgc tttctctctt tctttctttc tttctttctt cctttctttc 30600 tctctctctc tctctctctc tcccttcctt ccttccttcc ttcctccttc ctcccttcct 30660 ttcttctttc tttctttctt ctttttcttt tttctttttt cttttttttt cttttctttt 30720 tgttttttcg tttttttggt ttttttgaga cagggtttct ctgtgtagcc ctggctgtcc 30780 tggaactcac tctgtagacc aggctggcct taaactcaga aatccgcctg cctctgcctc 30840 ccaagtgttg ggattaaaga cgtgcgccac cactgcctgg tctactttta ttgatttttt 30900 gatattttcc ttccctcaca aagaagttga caaacagcct ggcagagctg tgagaactga 30960 atggagactg agtaggaacc gtgccctgga tggtgcttgg ctctcatacc tcagatcctc 31020 agggaaatgg gctggactgg ggcctagagc actgaatccc ctggaaggcc cctgttgaca 31080 aaatctctcc ccaaacccga caaaagcatc acaaactgtg ttcttcccag cacctacagg 31140 agctacacct tagaacacgg atgtttccca ggtgttcttt tactgtccta tttctaggaa 31200 cccgggactc gcacttgttt gaactctagg gcgtttaaga agtcactgat aaggcagagc 31260 cagatggagg tgggatgagc aagaactgct actctgaaga gagggacagg tgcacagaag 31320 actaatggct cagtagacag aaaacccacc tctatctaca gataagccag gtgacaacat 31380 tagcctcctc ggcagctgtc ccctcttctg ctgagaccta tcgattgaca tcctaaatgt 31440 cactgtgaaa tgttcaaggg atactcccca cttagcgtag ctgctgagcc agggaaggca 31500 actgaatgtc ttatttgaat gggttgcctg gtgctctcca gaaaggttcc aggctgggac 31560 ggaaccttcc caagtgaaga aactcacttc acaagcctcc agtccccgcc ctgcaacaag 31620 cctgtttgtt aacttgaact tgcggtgaca cacaccgagt tcatttccag tttgcgctcc 31680 agggcaagtc cttgactaca caaagagtga gacagttggg gaatttgtgg actgtccagt 31740 taatcagata aaacccgaga gagtcagaaa ggcgacttct tggaagatgt caaagtttta 31800 ctgaaaacag cggaagtgtt cgcagctaat tggtgctttc cttccacaca ccccgcactc 31860 cctaccgccc cctccctccc cgcctcccat ccctgtccag tccccacccc ctgaagtttg 31920 tctttggtaa accccagcta cttctctcac ttgtctctga ggcacaatgg gagtcttcat 31980 ctgaattcta gggaagaaaa gatatgactc cttctagcac gagtgagctg gattgtaaca 32040 gggaacgaat tgattgattt ttccccccct tcatctcaat gaacacacta tgtagccaaa 32100 ccaaggctga tcaacaacac tcaataaaaa aggagattca aactgaggcc cagctgagcc 32160 ctactagacc tccacaatgc acgctgtcat ctgcttctta catgtcatca catttaatta 32220 tattaatctt cgccataagt aatggagggg attattatga gccttggtct agcaactggg 32280 ttcaaggtca cttagctaat atgtaactct aaatgttaca agtttctaag ccaaggatat 32340 gtccttttct caggccgatg ctgtcctgct ggccttctaa aatgttagtt ctctgcctct 32400 tgttctgtgc ctacctcagt gccccacccc cactcctgct cccccccccc ccattattct 32460 gatcacattt acattcccgg tctcccattc tcctgatcct gtgtgcttac atcagctaga 32520 aattatatgc aggactttta aagagttttt ttcatagtat tgcctttttg catatatata 32580 tagtgttctt tgtttgtttg tttgtttgtt tgttttcaaa acagggtttc tctgtataat 32640 agctttgact gtcctggaac ttactacaga ccagtctggc cttgaactca cagacatcct 32700 cctgcctctg cctgacctgt caagtcctgg gattaaagct tgcaccacca cacccagctg 32760 cttttatagt tttttttttt tttaagttgt gtggtgtgca tgttgttgtg ttttataaaa 32820 tagctgtggt ataatgtttg gggatgggga acatttcagc aggcctctgc caaggcatac 32880 cccttgagga atcagtcata ccacaggctt agtataaaag ggatagggga cctggaaaag 32940 gggcagaggc agagaaggtg ggggacagag gaggacagaa agagagagag gccagctggg 33000 aacatgttgg ggagagatag agagagggag gaagggaagg agaaaagaga agagagagag 33060 ggggagggag agggaggagg agatgaagag gaagggaggg aggagagaga gagagagaga 33120 gagagagaga gagagagaga gagagagaga gagagggagg gagggcaagc agtccttttt 33180 atacaccagg ttggctcaca cttggcaagc ctcaagctgt tgctaggaag ctgttggatg 33240 gagcctaaag gaactgccaa tagtgtgtgt acagatgagt ttgtgtgtgc gagtctatgt 33300 gtaagcatgc agaggccaaa gatatcgttc tatatgacag ggcctctcac tgagcttgaa 33360 gctagtcatt ttccccaggc tggctggcca gcaagctttt ggaatctgcc tgtctctgtc 33420 ctccaaagat gtggttacag ggatacatgg tagtgcctgg caatgcctgg ctttttatga 33480 ggatttgaag tcaggtcccc ctgcttgcat agtaaatgct cttacccact gagccatcac 33540 ctcagactca tcttattaat tgtttcagtg tcgctgtatc gtggacacaa acatatgcat 33600 gtatataata gggggagagc agattattct ggcatttcga gatcctagcc cagtgcaggt 33660 gggtggcagt attagcaaaa tcactctgat ctgatatctg tttattcatg tattcttatc 33720 taagtgttgg aagaagaaat ggcacgagaa agcacagtaa cttagttcac agtcacaaag 33780 cttggcaact cctagctggg tggtgacacg ggctgtaccc ctatcattca ggaggctcaa 33840 gcagaaggct cccgagatgg gagctagcct gggcgttgga gtaaatccca gggtaacttt 33900 ggcttgtgta gtgagttctt gttttgagag actcacgtct ggggagccag agcagtagct 33960 cagtggttaa atgtactttc tgtgaaatca taaagatggg agttcaaatc ccagaacctg 34020 tgtactgaca tactggggaa aacctataag gccagctctg gagggaggtg gaggcaggag 34080 gattgctgga gttagttggt gtcttagtca gggtttctat tcctgcacaa acatcatgac 34140 caagaagcaa gttagggagg aaagggttta ttcggcttac acttccatac tgctgttcat 34200 caccaaggaa gtcaggactg gaactcaagc aggtcaggaa gcaggagctg atgcagaggc 34260 catggaggaa tgttctttac tggcttgcct cacctggctt gctcagcctg ctttcttata 34320 gaaccaagac taccagccct gagatggtcc cacccacaag gggcctttcc cccttgatca 34380 ctaattgaga aaatgcctta cagttggatc tcatggaggc atttcctcaa ctgaagctcc 34440 tttctctgtg ataactccag ctgtgtcaag ttgacacaaa actagccagt acagttggct 34500 tccagcgcag acaggaaaat ttgaatcttg attcagaatg agatccgtcc tcaaatgaaa 34560 agccagaaag taacagagga ggacactcaa tgccctcagt gggcacgcgc acaggtttgt 34620 gtatgtgcac agaagatggt atactattac catacttgtt tgtttgtttg tttgtttgtt 34680 tgtttgagac atggcttctc tgtatagctc tgggtatgct ggaccaggct gacctcaaac 34740 ttacagagat ctgcctgcct ctgcttccag agtgttgggg ttaaaggagt atgccaccac 34800 cccctgtgga cacagatgta ttcacaaatt ttcaaattgc atctgtttgt ttggctagtg 34860 cacctggcac agagcgtcct ccacatggca tggatgaaga cttattccaa ttggttattg 34920 atcaaactaa ttcatgaaaa agacagctgt ctttcacctg cccagacaag gagcaaaaat 34980 tcatccttat cttatggatt gtgcaccttg cacaatccat acaaatattt tttataggaa 35040 taaagatttg gttgtaagat tcatatatta cagaatgaaa cagggagata cagctctgac 35100 aagattcatc cttggggatg ctgagatgac ccgctgtccc agctcgctgc cacatcctgc 35160 ctcacctgat gctgtttcca gagacctgct tccagagcag cgtcaggggt ggctgctgat 35220 tcccatgaca tcagttcatc cagcctttca gacagatcca ccaagacttc agttaagccc 35280 tgaacattct aagcatacag ggactggata acaaatgcga aaactagctt tcttaactct 35340 aaccaagttt tcttatttcc ctacacctcc cacccccttg aaaaaaattc atcaccccaa 35400 ttcagcagga agtagttatg gagagttgtc atcttgattc cttgggttta ggtgtctggc 35460 tatggttatt ttaacattat tatagatatt ttaattattt aattattatt aaattataga 35520 tattttaaca tattaaggga tactttggaa atgatcataa ttttagacag ggaggaaact 35580 agagaggtta gacttggaga ttctgtcttt ttttttttct ttcctttgct ctagccttac 35640 tttcttaggt aaaaggaagg gagctgaaat gaaaatgggg actagagtaa tataggaaat 35700 tagagatgtg agatggatta ttcaatttac tcttaaacaa aacattttat agttataatc 35760 tttcattggt agaaatcttt acattttgat gcagaactaa agttatattt tcttacattg 35820 gtacagaatt tatatatcga tacaggttta aggttttcac tggtataaat ctttgtatat 35880 tgatataaaa tttaaaatta attctgttac tcttagacag gcattgtgcc tatacaactc 35940 atttatacaa tacaaggctt agacccagtc cttctaatag gtactgtcac aaatggttta 36000 agatgattaa gtgatgcaag ttaatggtca gatagtaaac tcatgctcat attagattct 36060 gatttaatta caataaagtg ttttcaagat tatcacgtag gaaataacta agagtaatca 36120 atgtttaaca attcaattat attacatata gatagatggt cttcaaaaac atcagaggtc 36180 cacaaaatat ggcatttaaa atcatttcac tattaatagg tcttttgact aggagacatg 36240 tctgctccta acagtacccc catctcactg caaagaaggt actgagtatc aatacctccc 36300 aattggagtt gctttgattg cgatgagcta gctgctggta agaaatagcc taatttaatt 36360 ctacagacaa ttactgtcca gaaaggacaa atggatgcag gacagttgac tgctaagctc 36420 tgccaaaaca gagtaagcta gtcctttata tttcctgctt catttatggt ctatcagatc 36480 gttctggacc agaagtctga agacaggtgc tccaacatta taaggaatat tgaggactgt 36540 ccaggcagtc agttgttcct acaaattgta ttaagtttta gaagctatgt ctttgtgctt 36600 tctacatatt ctgataattt taaattcctt ctcagatctc tgatggaatt gaaaaatagt 36660 tatagtctca taattgaact taagttattt agcactaaag aagatgttct agatttgaaa 36720 ggatggtttt ctgagggtat tgcaagttaa aatcaagcat aattgaggta cagatttgta 36780 aattctgtaa gttaagatag ataatagagt actttatcta aattgtcaga tatgatgtac 36840 tggatgttct aagtgtatat cataccttac aatttacata cttgttataa ctgtgttcaa 36900 tgtatatttg ggagaaaaga gtttttaatt ggacaaaagg ggggaaatgt agctgtctaa 36960 tgctaattat gtttcctcat aactatttgc atgagagggt ctgcacatag cttctgggaa 37020 ccttcccaca gttaattctg attggtaaat aaagttccca gcagccaatc tctggggaga 37080 agaaaaagag gtgggtgggg cttaggtttc ccaggtgtgg gacctctggg aagagagaag 37140 gaagagagac tcaatgcctg aaaggtagta taggacaaag aggcagagcc accctgtaaa 37200 ggggccagga gagagtggcc cagatggctg ctcaattggg ccaggagagg ccaagatgga 37260 acatagaaat tggtaagtag taacttggaa ttattggcgg gaggtagagt ctagcagtat 37320 ggaggctagg cagatgccca gctattgtgc tgcttaagtc atattaaaat ataaaggctg 37380 tgtgtgagtt tttcattaga gatcataaac cattgaggta ggaagtaatg ctgtgctggg 37440 atttattaaa acattaatac tacacttgtg gcaatcacag gacagacaac ttgtaggaat 37500 ggattttctc cttccaccat gtgggtctag ggaattgaat ttaggtagtt tacagccttg 37560 gtggcaagca tgttcaagcc agccccaatt taatgatttt aattaaagtt gttgtcctct 37620 gaggcacaaa aaaaccccaa agggttcaac acaatcagga ttcctgctgg cctctcatct 37680 acagcttggg ccagaagcct tgctctgttt tgcatttgga atattttatc tcagtctctg 37740 taagtcaggc ccagggatgg gtatagtcag tacacatgaa tttggtctgc ataactggaa 37800 atgtgattat agcttctcag ctgttccaaa ggagaagcac catcatatcc cattctgcct 37860 ttgtgtgaaa aactcctcaa aaatacatat tagtttgtat ctcatatata tgtatacata 37920 tacacatgta tgtatgtata tatgcatgta tgtatgtata tatgcatgta tgtatgtata 37980 tatgcatgta tatatgtata tatgatgtat atataatgta tatatgatgt atatatatca 38040 cagacacata taatttaggt cactttttgt ccctatgttt tatttctttc aaaacctact 38100 gctgcgcctt acacagtgtt ttgagaccat cttctttctt atcttttttt tctcacataa 38160 tatatcctaa ttacaatctc cactccttct agtcctgcta gttcctcccc tcctatccag 38220 agccaccttt ctgtctctaa ttagaaaaca agcttctatt atatagatta taataagaca 38280 aaacaacacc taacacaact gaataggaca aacaaaaagg cagaaagaaa agagaagtca 38340 caagaaatag atccacctgt tcacacactg aggaattcca tataaactct aagctggaaa 38400 ccataatata atgtgcgaag gacctttagg gtaaagagag agaataaaac tgcataaata 38460 aaataaagta aaaataataa taaaggaaaa tccctgacac agcacagtaa gacagggaag 38520 ctccaaagac tctgttgagt tcattctctg ctgaccatct agttctggac atgtaaccta 38580 cccttaagag tcgtttgttt ccacagcaag actgtcttag aggaaactaa attttcattt 38640 ggaaatggtt atcaactgga attgcttctg ggttaggcct aggggcttgt gtctacatct 38700 tctctcagct ctagggtcca atcttgtcca gacctgtgca ggtccatgca tgctgcctca 38760 gtctctgagt tcatatgtgt atctatcggc ccttgttttc ttggtgtgcc ccatcccttc 38820 ttgctcttgt attttgcctc cttttccctg agccccaaag gaagggatct gatggagata 38880 gcccatttag ggcagagtgc tttaaggtct ctcatttctt acaaatatct gtctgtgagt 38940 ctctgtgttc ccatctgcta agggaggaag cttctctgat gatggctgac aagacgccaa 39000 tcaatgagca tgtcatcagg ggtcatttta tcgttacatt tttgtttgct taaaaatagt 39060 aatatttggt tctaccctag gttcctgaac tagcttgtct caggttcttg gttacccaag 39120 cagtgtcagg tttcatctaa tggagaggac cttaaatcaa atcagatatt agttggttac 39180 taccataaga tttgtgacac cattgcccta gtgtattttg caggcagggc atcattgtag 39240 atcaaagagt ttgtggctgg gttggggttt acctctctcc tttggtagtg tgccttccca 39300 taccaagaac tctggtagat aaaggtgaag gctctatgta ggcaccagct caatttctcc 39360 acgttcaatg ggttgtgtag gtgttgtctt cagcaatttt tagggtcact tatatatact 39420 atcatatcat ctgcaaataa acacactttt tcttctttgt ttccaatttg tatcccattg 39480 attttcttta gatgtcttat tgctctagct agaacttcaa gtgctatatt gaaaaggtat 39540 ggagagagta gacaaccttg ttttgttact gctttttagt ggaattgctt tgggtttctt 39600 tccatttaag ttgatgtggt cttgctgtaa gctaccttta ttatgttgat ttatccctaa 39660 ttattccagg acttttatta tgaaggagtg ttgggtttta acaaatgcct tttctgcttc 39720 taatgagatg acatgtggtt ttgtctttca gtctgttaat acgatggatt acatttactg 39780 attttcatat ccctgcatct ctgagatggt gcctacttga tcacagtgta ttatcttttt 39840 gatgtgctct tggattcatt tgcaagtatt ttattgacaa tttttgcatc tatgttctgt 39900 ttgaatttgg tctgtagttt tctttccttg ttgagtcttt atgtggtttg agtatcaggg 39960 tcactgtgac ctcataaaac aaattgggca gtgtcccttc tatttctact ttgtagaata 40020 atttgaggaa tattggcatt aactcttctt tgaaagtctg gtagaattct tcactaaaac 40080 catctggccc tgggatattt tttttttttt tttttttttt tttggttagg agacttttaa 40140 tgactgcttc tatttcacta ggggctatag gtctctttaa attacttagc tggtcttgat 40200 tcaactatca taagtggtat gtatagagaa atctatgtga gctgaagaga tggctcagca 40260 gttaagagtt tgctgttcct ctgtaagacc tgaatttggt tcttagaacc cagattgggt 40320 aactgatagc tgtctgcaac tgcagttcca agagatataa tactctctct tctagacact 40380 ggacatggca cacacacaca cacacaaagt tgaatgcaaa ttaaatggta gataacggta 40440 tgtgcacttc agagtttgtg gtaagtgaca gttgatttac ttgatgcaac acagagtcgt 40500 ctggaaagca aatcttgtgc aaggatcgcc taggttaggc tggctatggg caaggctgtg 40560 gggaattgta ttagttggtt acttgtggta agacctatcc tgattatggg tggcaccacc 40620 tcatagtctc agtcctgggc tgactgaaaa ggagtaagct gagccctggc atgtaagtat 40680 taattcttgg ctctctgctc ttgactatgg atatgatctg actatctgtg ttaagttcct 40740 gctgtcccca ctgcccagga gtgatggccc tagcctgcaa ctgtgagcca cagaaatccc 40800 ttttctctta agttgctttt atttcggggt tgggtggtgg gcggtgagac cgaggttttg 40860 tttttgtttt tgtttttgtt tttgttttgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 40920 gtgtgtgtgt gtgtgtgtcc gtgtgtgtgt cctggctttc ctagaactca ttctgtagac 40980 caggctgacc tcaaattcag agatgggttc ctttgaggca agcaacttct gaaggctagg 41040 aaggctcaag cacattggct cctgggtcca cagtgctcag catctgtatt tcatgaacag 41100 ttcctgggtc ctctgccagg gctgtaagaa gcacggtgtc tgcttaatac aactgcagcc 41160 aaagaagaca gaggtgttgg tgcaaaaaca cagctctcac atcaaagcag gtaatagttt 41220 attctgaagc caaatatgag tcccaatggc ctagtaatac agatttaagt tacccagcat 41280 tctgtgtcca atgtgctaag atttggtaaa gtttttatgg aagcagaaga agtttattac 41340 aaatccgggt acttttcaaa cacattggtg gaggcatcgg gtaggtggat cacagcaaag 41400 caggaacaac tttacttgag gctcagatgc tgacagtgtt tcacagccca ttggttggcc 41460 ggaaactaag agttcgcaca ttctacggaa tttacctaat ggcacatgga agggcaataa 41520 atggctgttt aggatcctaa agatgcccca agataatttg gctctagact tgcaacattc 41580 taacccctct acatcattga cattctaatc agttccgtca gcctttccag ggctgggggg 41640 taggggagga aggcaaggct atttgaggaa tagcttcact acttgaacac cacacattca 41700 gggctgcaaa ggacacacaa ggctctggtc tgtggttact agacctctca tcaattcatg 41760 aggttgtagc tatttcgaac tctggaccat cttgtagagg aatgtgaatg aggctgaatt 41820 tccgacatgc ctttggacag atgttcgtga tgctaactct agtttttgta agatagttat 41880 tctcttctgt gaagtgatga agaatttgac acagttttaa agaatctccg tctctctgac 41940 tccgtctctg tctcaatctc tctctctctc tcttcaaatt agaggtttgg taatttaggc 42000 acagggctgc tatatgactc tttgatagcc tcacaggcat gtgtacttag tgtgatgctc 42060 agcactttga aggaaggaag gagggaggaa gggagggagg gaggagagag aaaggtgaaa 42120 ggagggatga aggagggagg aaagaggaaa ggaggaagca aggaactcag ttataggcca 42180 aagagttttc aggtttatag acacatagat ggttgcagct gtagcctggc agtctacata 42240 aaggacacat aaagacattc tcttggccac aggttgctaa ggctgggtag gaagtctgta 42300 gaaggaagcc agaagtcagt aagtcacaga ttaaacattg agcaaaggac cacatcatta 42360 gtcactcaat gctagtgttt atttgtcttt ctcttttgtt tgtataaatg gaagtactct 42420 ctagggtttc tgtttaatta aaaatcttat taaatataag caatgaaact ggcttaacca 42480 ggaaactgaa cattaaaccc gttcacaaaa cgtatgatct gactaggaga aagagaaatg 42540 tgctacccac accaatcctg gttggacatg tgcctaagcc gaggtcacta ctagattcag 42600 tcagtaccca gagaaggtag caaacagcct ttgccatata actgaatgaa gggccagcct 42660 catatctggg tttctaactg cagaacacat acatgggtgt gtatcccagg tcaggttgtg 42720 ggtggcaagg ccttgctgct gtataggggt cagggctctt cttgcatatc tgtgtttata 42780 acaagagttg cttctgggtg gcacagacta ggggagacag ctcctccttc tagtatcttt 42840 gtcaagggaa cacactataa ccttccatgg tggttagctt aactatttcc tgtgggtgtg 42900 tcccttatta aaactatatc ctaaatatgg tcctctctta ggaggcatcc catttcctag 42960 tcagtcatca tacatgatat acaaagagag gtatcagggc ctgggctcca tggctgtcta 43020 gatgtttctc atctgttgtg ggggatggtg cagcatgccc tgagctgcct gagtcactgc 43080 aggaatgagg aagactcaca gggaggaggg accaatttcc ttcttcagta gagtcagaga 43140 gctgcatagg tgagatctga gctgtgcaag gagtgggcag ggcttagatg tagaaaattt 43200 ttaatcctga cctggggctt ctaccctgcc tttgactctt tagttcccag atagaagaca 43260 cacacagcct ttataattac aataaacctt caacagcaca agagctgggt ggatacttac 43320 cctctacgtt attagaatct actttcctat tgataactct gaattattac ttactactta 43380 actatgtttc atctgggctg ctcttaactc caattggcca gccctcaggg ccatgttttt 43440 atgattccca aaccatggca ccttctcctt tgtcttctgt accttctagt tctccttggt 43500 tctcctctga cccatgaggt caccccaaac ctctaatccc tccagtaatt ggctgtagag 43560 aattttattt aaccagtagc tttaaatcaa gggacaaggt ttgcagaaca caagcttgta 43620 aacaggagaa gtcttttgta ggccaagacc tttggctata gaatttagca ttacaatgca 43680 tagcatcaga ccaaacctca acacttagaa actcagcaag ggacaatgtg ggcaaagacg 43740 tggagtgaga gccccctcag ggaccccatg gtcacctgtt ccccattaac cacaggtctt 43800 tgggattctg caaaccccca gcaaccccca gcagctgctg gaagcaccat cccatactcc 43860 atgagtgtgt atccaagctt tgcaattctg aagtcctatg agacaatgtg agtagtgtgt 43920 cattgtcagg attcatgtat attgtagaca caagatttaa tgaaatcttt ctattgtcaa 43980 aagtgtttct aaaatgtttg gaattacatt agagcagaaa gagctccaat ttatgttttg 44040 ctaggtacct tcccaactta gtagctttat ttatttattt atttatttat ttattatttt 44100 tgatcatgtg caggctccca aagtgcatgt ggaagatcaa aggacagatt tgtggagtca 44160 tttctcatat tctaccttat gtgggctcca gagctcatac ttaagttgtt aggaatgtga 44220 ggaaagtgct ctacccattg atctatcctg ctgacccttt agccatttta aatagcaacc 44280 atttctgaca gcatttggca tctgaagatc aagaattcag gggctcctta gctgtgtggg 44340 tctggcccca ggctctcaaa aggcagtgag cacatcttgg ccaaggttat gtcacttgct 44400 gttgaaagat caaattcttt gggttcagac tccatcttag acaaacttga cttaagtaaa 44460 ttaacaggtc ggtacctagc tccagtttcc agattaccca aaacaaaacc tgagcttagc 44520 tccagtttcc aggctacccc taacaagatc tggatctcca gtttattctc ctctaacaaa 44580 tccactgtct agcaccaatt tccaggttaa tccccagcaa atcccaacac ctccaggtta 44640 caaggccgcc cctgcctaac aaccaccaat taggaggaaa gccaacgttt ggctcccagc 44700 accagccaat tacaccaaag gtcacagtag caccccaatt agatgcttgc taggcttgaa 44760 acactcaccc ttcacttgtg atttcctata aaaccttgtc ccagtgagtg atcaaggaat 44820 ttttccatgg aaccgtcctg tgggtcagca gtgaatcaag acccacgctc gagcttgtga 44880 ataaagtccc tggtgtgata gcatcggatc cggctccttg gtggtctttt ggagttcatg 44940 aacatttcag catgatactg tttaactggc atgggcattc ttcttgtggt gggcatctgt 45000 tcttcaaatg gcgcatctct gtgtgagtga gtgtctctat ggcatggtgg ttggtcgcca 45060 ctgaaatgac ttttgtagaa gagcaaacag ataaaaggtt cttttgtgcc gtagtctctg 45120 tcaaaccatc acttctgctg tattttatcg gccaccctga gcatccttga tttgatgcag 45180 aagaggatta tgcaagcaaa taaataccag aggatgtgta ccactgggaa acaaggcaag 45240 gtcacctctg aattcatatt ctgggaagat tagagctttc agaacaggag ctgggcacat 45300 gcctgtaagc tccacactca gaaggccgag cagatggttc acaccagtct cagttacaca 45360 agtcagttaa ggtcaacctg ggctacttga caccctgtcc ctacaacaac caaatataca 45420 cgcatgccag agatgatata gtgaactaga caccctaaaa aaatttcacc tttaacgtat 45480 tgaaaaatac tgagtagaac attttaaaga tttattttgg tttttaaatg ttatgtctgt 45540 gtatatgtgt ctgagtgtat atcgcatgag tgtatatcgc atgtgtctga gtgtatatcg 45600 catgcatgaa atacccacag aggacagaca atgtcgccag aactctaaaa ctggcgttac 45660 agacacctgt gatccaccac gtgggtgctg ggagcccaat cctgatcctc tgcaggagca 45720 acacaagctc ttagtcactg agccatctct ctagccctaa gagtcaacaa ataaacacac 45780 acacacacac acacacacac acacacacac acttaaatct ttaaaagctg ccccattata 45840 ctatcaggag aataacaggt aaaaccaaca caggaggagg tgtggattga gagactcgcg 45900 gagttgcagg ctctccttct cctgctctag ttcccagtag cttactattc atttaacaat 45960 gtttatggcc gtttgctagg cctattcctc atctaactcc taacttactt aacctgttta 46020 aactattcta tgtctgccac atggctggtt acttctcagt atcatatatc taatttcctc 46080 agagtgcagg tggtgaacct cccagagttt cagtctgtct actggaactc ccagtttcta 46140 cttcctgcct ttggcaaaaa acaccatgcg gcatatttgg ttttacccct tgacctagaa 46200 aacctcaagt gtttatttcc tggcccttta ttttctaaaa tgccaataag ataggccacc 46260 agatctttaa tgacaggtga tgcttcgaca cagtacctga gagattatct ctacagacat 46320 aagtgcttaa gctgggggct cctctaggca catccctcaa ccaccaagtg acagggaagc 46380 ttggtgagtt tctttcacat ggcaacaaac aaggcacctc agctagcaag caggaaagca 46440 ggagataaca tgtgacggat gtaggcgtca gagcaaagac ccttccagaa actgcctcaa 46500 atctcaatgg atatttctca gtgatagttc acatcaggct tttttttttt ttatgagtac 46560 tatttttctt ggtttattca ataggctgct tcaatttacc ttaatgtcca cttatactta 46620 tatataagct tcttttatac aagctcagtg gactcactga gaaagcacaa ctaggaatgc 46680 aaatcagaca gcaatattaa tacagagttc accttctcct ttcaatggaa taaataatgc 46740 gtgcatttgt ctctttctaa atatttaggc accaaaagtt ttcatgggac tctttctttt 46800 ttattttatt tacttattag gtattttctt catttacatt tcaaatgcta tccccaaagt 46860 cccccatacc ctcccccctc ccactcccct atctacccac tcccacttct tggccctggc 46920 gttcccctat attggggcat ataaagtttg caagaccaag gggcctctct tcccaatgat 46980 ggccgactag gccatcttct gctacaaatg cagctagaga cacgagctct gggggtactg 47040 gttagttcat attgttgttc tacacatcag gcatttctta attctctttc cacatgcaag 47100 gagataaaac ctttcctact tctacagtag ctctgatggt cagggaaagt caggaattat 47160 aagcagagtc tgggtttata tgggcttgca gccacgctca cccagcatga gttacagaaa 47220 cctggctgat agtctgtttg gaaatgtgac ccagcatgag aagtgttctc tgtctagaaa 47280 caaacgggag tcctgttgaa gcacatgatg tctttggttt tgccccttcg tttagaaagc 47340 ctaaaaagat tattatctgg ccctttattt atttttaaat aaatgtcaat gttagttgca 47400 gaaatacctg catagaagcc cccagttagc ctagagttga aatacaggaa agatgcattt 47460 atcaaactac cctgtgcagc agaacaaact gaattagaga ccggggcagg aggaagcttg 47520 gttagacaca gtcttaggct tggttaaaaa aagggggggg gggcgttatg cattatgtac 47580 agttaactgg gtacctgaaa tagaccagac ttgcttctga gcactgaggg cttaatggtt 47640 aactataagg agcctaggga ggaagattgg ttcgagaaca tagttactcc tcaattgtga 47700 atggagttct gcaaacagag aacattaaga ggaacagaaa aggagaattc ttttggaata 47760 atagctctct gccacatgta cccaggtgct ctgatcccca aatacatcaa agctgctttg 47820 tcacccagtc tgtgccagaa tgggcttgca caatgcatct gagcaccgag tgctaatatg 47880 ctctcctgag agcagcagtg gggagagtct gagctggtgg cagtgagcta gctgcagatt 47940 gctctgggct ggtggcagca ggacaagtca attaggggag tgattgggag aaaggaaggc 48000 aagaggcacc cagagcctcc aggctcttgt ttgaaaaatg gaagacacac ctggcttttt 48060 ccacatttcc ctaggtgctc gctagggcct ggtcacctgc tctcctgatt gactcagcag 48120 ggattgggtt gtaaaattag gggctgggtg atgaggcagc aacttccgag tgcctattct 48180 gtgcatgtct ttgtacattt atcaaggccc tcaaatcacc ctcacagagc agatattggc 48240 cccttgtttt gcaaataggg ataccgaggt ccagagagag gtactaagac tacaaaggga 48300 gatgccaccc ccaaatcctt ctttctttat ctccaagtaa ttctagccaa ggaccacatg 48360 ggagggtggg tgggctcagc ccagctctcc ttctaagaac aaaaaggact tatgatgtcc 48420 cctgggctcc ccatttctct gtgagagttt ttaggctcaa cccatgaggc ctagaccttc 48480 ctgagaatgg acaggcaatt tatggtagct gaggaaagga ggaaacattt tctttagctg 48540 tgtgactgac cactgatgag tggcacatgt gcctataaac tcactgggta acatacacag 48600 aggggcatga aaaaaagagg ggggctagtt gagaagaagg gaggggatca ggagtgggag 48660 ggagagaaga aaggggagcg atgggtgaat atgatcaata tacattgtat acagggatgg 48720 aaatgtcatg ataaggccat tattatatat aattgataaa tgcttttaca aaatcagaac 48780 agaaagataa gctgaggagg aagttcagaa tcacaaataa gtaaaataac aacagggaaa 48840 aatctttctc agaacgttgc tatggagata gccacatgct gtgtgagtgg agacctcagg 48900 ttttcctatt gggcaagatg cattgagtag ctacagtcag ggaaagccag gaggtctgaa 48960 cctcgatgcc agagagccca tgcagagttg agaacaggag gaaggttctg aggagcccgg 49020 agggtggatg tgggtgacaa cttcagaaga agaagggcag gctggaagcc agtatatgga 49080 gacagatgtc attcctggac ctcacggcta ccttgctgag ctcactttct gccagcagga 49140 gaaacatgcc ctgaagatgt gaagatgcca gagaccggta cagaaaagcg agaggagcgt 49200 tgttgtggtt ggttgtatgt gctgacttga tcaggctcca gggtgctcag gtatttagaa 49260 aagcatttgt aatggctgtg tctgcaaggg tgtcttttga tgtgtgctac cgtgacctgg 49320 cagagtgaag tatacattgc tctccccagc cctggtggac ctcatccaac ccattggagg 49380 cctgtgttgt gcaaaatcgc agaggtagga aaatgtcttc cctctgccta tttttgagcg 49440 tgagcgtgaa tgctcttgcc tgtggagtaa gacatagact ggaccttacc tgtgttagac 49500 ctccatgctt cttggattag gatttggact agaatttatc agctgttctg ggtttgctgt 49560 tgtgacccac aggtcatcac actcctcagc ctctgtagca ctgtgagacc attagttata 49620 agaactcctg gagttggaga gatggctcag aagtgaagaa tgcttcctgc tctttcagag 49680 aactggaggt cagctcccag cagctgtatc accatcacgt gacttacaaa cacctgtacc 49740 tccggttcca agggatcaag tgccctttct tgcctctgtg ggcacccaga catgtgcata 49800 aacgtacaca gatatacaca taaataaagt aaaataaaat aacatgaatc ttaaaggaag 49860 aaacccagtc aatcatcacc catcctgcct gcatcctctt ggttctcttt ttctctgggt 49920 caaatggatt gccccataca agcatcttct tgctgccttc tctacaggct ggggctaaac 49980 agggaagtta tgggtgggga caactttcag acagatcaag aaggaggaag gagcgactca 50040 tgattttcaa agacaacaca aatgttggca gtttggggat gtaaccgatg agggcagtgg 50100 actcaggggc agacccgtgc gcatgcgccc acgtgttgcc atggcaccaa caccatgcgc 50160 tcaatgggat ctagccacac ttacgttaat gctctggtac tacagagttg gaacttttag 50220 taagtttgag cgaggagtaa tatatcagtg gtgatgagca tgcagggtta ggtcccttag 50280 agaaatggtg actagggtgg gaagtgacac agctgttgcc atatcaaggc tagagagttg 50340 ctccaccgaa tagccataag tcctagtatc agcctcagag ttcaggaact ctgcttttct 50400 tgtttgtttg tttgtttgtt tgttttcttc agacgattct gtgaagggaa gatggaaagt 50460 gttaattata taaactgaag acgtttccct gtgaagtaag gccagcccat acccagcctt 50520 aggctaagtt tcacttgccc tttcgcaaca caaacctttg tccctagatc tgactaagag 50580 gttaagcaat gtgaataccg gtggccaggc ccttcctctc cccagaagag agaagaggtc 50640 cccgaggcag cagatgtgag tcaagtgcct tgtggtagag gaggccactg gagtagacct 50700 gggaaaaggt tctgacactg tttatgactg ttgggctggg cttgatcaga ccaagcacca 50760 aacgcagagc tatgtatgcg cagctctgac cttcctccag ggaggatttc aggatgggtg 50820 ctcatgaggc tccactcctc tatgagtatc ggtaggcagg attaatggtt gccccagaaa 50880 agtaatgttt tcttcagtgg tgtagctact gataagttgc ccatgtcatt gtaaataacc 50940 cctcactcag gctcttgtga ttaattcact ggggcacatg acatcaatga catcacacat 51000 acacacacac acacacacac acacacagag agagagagag agagagagag agagagagaa 51060 taataaaaaa ataaaaattt aaaaatcatc tagtctttgt taataaggat aatatacttg 51120 gtggtatact taaaattggt ggcaaaaaat catgcccgta agtaagcact gtctgagggc 51180 ttgcgaaagt ttctaaacct gtagttacag gcatcctcaa caaaaggcat ggcaagtcat 51240 tgacattagt gtcacccagg cacacagcca actcaggtgg gaattgttgg ctctgtaagg 51300 acaggcaaag gaaaaggtca cactcaaatg gctcatctct gtcatcctta gtagaaaaaa 51360 ttgaaaatga tattagtcaa gaaaggacta aataagatga tatgcctaat attcagggag 51420 accaggataa tgtttcaaat gccctggaat aactggctct gtgtgtgtgt gtgtgtgtgt 51480 gtgtgcgtgc acgcgcgcac gcacatgtga gtgcacagat ttatgtctga atggtcttca 51540 gaaattaggt gatattccac aggaaggatt ttccatccta tcaagaacct aagaatattt 51600 caaggaacca gatcactatt tagggaagag ggaaggtcgt gtattatatt agccagatat 51660 acttcactca gatatgattt tggattacac atggtggtgc acacctttaa tcccagtact 51720 tgagaagcag aggcaggaca ggagacaccc agggccaacg agacccacat gaatgcacac 51780 cccctcatgt gtcagtgaga gctctcgcac actctccctc tcataatgag aaatgagaaa 51840 aggagaaaga taaaatatct gctattcctt gtattagtca aaaaacttcc tgcgtttgag 51900 gatagcttcg tgtgtactta gatatttaga aagtctcagg taaaccagtg cttaatagtt 51960 tgtctcaaac aaacaaacaa gcaaacatga tttgaaccaa tggcattggg taggaaggct 52020 ttgggcttca gataacaaag ctgaaggaag gtctgctcta gccacagctg aacgtccagt 52080 cctcttctct aacaggaagt ctgcaggaag ttgctctcca agtccacgca gcacttccag 52140 ggcgtaagca gctcagagtc tctgaacttg cctgcttgtt tcgctgcttc ctcttccact 52200 ccaggtggga accacggctt tgagcgacag agtctcatat gtcagcttct tgaggcagga 52260 gagagagagt gataatgcta gaggattgtc ttcccccttc cccccccccc ccgccccact 52320 ctgacaggaa gagccttcgc caccgtgaca acctgcctgc gatcatctta acttagtgga 52380 tagaccctca gacgagtcac tggctgacaa atatagacac ccatgattgg cttacagcat 52440 agtgttcatt gcttcctttc ttttccatct gcagtccagt ggggaccatt aaaactaagt 52500 caaagggaaa actattcatg gttttttaaa atttttttaa attttaaaga gagaaatatg 52560 aagggctagg gagatggctc gggggtcacg tgtgtgttgt acaagcatga agattagagt 52620 attatcttca gcaagaaggt aaaaaacctg gatgtggcaa gacctgtaat cttggcactg 52680 ggaggtagag accgaaggat cctgaggctt cctggtcaat cagcatagag cttcagatac 52740 tgtgagaaaa ccaaatggca gagagggata aaagaagatc cccggtgcta gcctcatgga 52800 catgcacaca agcatgcaag aatgcacacg cgcgcgcgcg cacacacaca cacacacaca 52860 cacacacaca cacacaatca taatgagagt agagaaaagt agaaagaaag acagtttatt 52920 attccttgta ttggtgaaac cctccactaa ggggcaaaag ctattgtaca ccatgaacac 52980 ctgcaggctc aacccttggg tgcagaggga gaagactgca caatgaattc cacaccaacc 53040 atggagaaac ataaattaac gaacaaatag gaaagcaagc aaaaaaaaaa aaactagact 53100 ggaaggaggg attctgggtc atggaaatat cattcgccat tgatgaattt tatttttatg 53160 ttcatttttc agctccatcc ctactcctta atgtcgctgc catgttcctg tgctatggta 53220 aatctcttga tttaaaccaa aagctttcct tacttccttt cagaatttca cttacgttac 53280 tctctctcct ctccctcact tacaatgaat aatcttggaa gaaaacatgt ctgcatagaa 53340 aaggactttc tggttagttc ataatgttgt tccacctata gggttgcaga tccatttagc 53400 tccttgggta ttttctctag ctcctccatt gggggccctg tgatccatcc aatagctgac 53460 tgtgagcatc cacttctgtg tttgctaggc cccggcatag tctcactaga gacagctata 53520 tctgggtcct atcagcacaa tcttgctagt gtatgcaatg gtgtcagcgt tcggaagccg 53580 attatgggat ggatccctgg atacatcact ggaaagagag gcccattgga cttgcaaact 53640 tgatatgccc caggacaggg gaacgccagg gccaaaaagt gggaatgggt gagtagggga 53700 gtgggggtga gggtatgggg gacttttggg atagcattgg aaatgtaatt gaagaaaata 53760 cgtaataaaa aaatattaaa aaaaaaaaaa gaaaaggact ttctacctta ctggcaactg 53820 taggctctta ggcgagggct ctcgcctcag tcatacgcgt cttcctctct gtaaagtgag 53880 ctggctaaat tagacacatc cgaaggtcac atagccccag ttctgaaact tgatggttct 53940 aaagtctgcg gggacaagca catgaccctt ctccaacctc tggtatcagg ttctgttccc 54000 actcgtttta ttcattatct gctctgtccc ctacttaacc cagacttatt tgtgcagtct 54060 ctgaatgagc aagccaggct tttttttttt ggatgatatt attagatgct cctgaatatt 54120 ctccaagtgc tttaaatgaa aactctggac agtacagcca gacttcccaa gttgccaact 54180 tgtcccaggc cttagaagtc acctaattac attgaattct gcctggtgga gatgaagaca 54240 gaaagcctgt cactaatggt gcatgtgttg agggtggtga ccactccatc tttattgcca 54300 gctatgctaa agcccttgct gcatcagaag gagtgtgagc ttgtaaagtt ccgagtctgc 54360 agcatgatct tccattctct aagcggtaag gcttaatgtg tgtgtgatgc tgaccagtga 54420 aggaagattc tagcaatatt ctgaaagtga tagataagaa atcaatacta aaatccatgg 54480 ctgagaatcc gtctttggtt aatgggtagc cacagaaggt gggagagaga tttctggata 54540 cttcaaggag cagaagaata agatcagcca gaggggctgc tctgagcatc gattgctgct 54600 gctgctgctg cccctgccgc cgccgccgcc gcctttgtcg tctttgtgta ttttgtggcg 54660 ctaaaagaat agcaagcacc aggcagcttc taatgaacaa atgcttgtga ttctggaggc 54720 caaatccaaa actgagatac tgacatctgg aggggccttc tggctgtgtc taatatcgta 54780 gacctcatgt ggcagaacca caaatggaga gagaaaacta aaagggtcca cactcactct 54840 tcactaggaa tccactcctg caatggtggt cgtaatcccc tcaccccatt ctttcagctg 54900 aatctttttt ttttttcatc aggttctgcc gcccaacgct attgcgtagg gagtacttct 54960 agcacatgct tatgggagtg tgttatcatc acaagagttg gtaactttca aggagacagt 55020 gtgagggaag gctgtagaaa tatttaagag gcaacagact tggtcagtca atgaagccag 55080 aactctaagg ggaagtgtga tgctcactaa aggaaacata aaggactcca cccctacccc 55140 ccacccccgg gtgaacagaa ctcttctgtg ctgtcccagc cttgcacaac agcaacactg 55200 ataccctact cccacagtga cagaaaagga cttgggactc ctatgaagga cgagggactt 55260 gactagagct cactaccttg acctttcact gttagaagtg gcataaggtg aagagctaca 55320 gcttaggggc tggagtgatg gccacttgct gttcttccag aggacccaag ttcagttccc 55380 agcacccaca tcaggcagct cgcaacaatt acccatgatt gctaccattg atgctgatgc 55440 cttcttccaa cttctgaggg tacctgcatt cttgttctct ctctctctct catctgtaaa 55500 aatcatatta agaaaatata tatttgggtt taaaatcaaa atggaagata caggttatag 55560 agatatattt agcaaaggaa ccaagaacac ttgatttctg tgccagagag tcacactttc 55620 ctcaaagagc tcccaaggag aaacaataaa aataaataca gctcttactt tgcactagat 55680 gctgttagga gcactttaac tccattagct gattttgttc ttataacaga tgccagggca 55740 agagaaagct gtatcagtca cttgagaatt gaagttgatt tcatggcagg cggtgggaca 55800 gagagaggaa agtgggctgg tccttacaaa gcacagatgc agaaaagtta cactgggaga 55860 caaaaatgag aagagatgca gagtagcctg gctagctggc tgggttcatt catttactca 55920 ttttaccttt attgagatgg ttagagggta agtctgtgct caccatagtg acaaactttt 55980 aaatgatgct tgtgcttcag ccccctccag tatcacctca gtcttatcat tcgaacactt 56040 ggtcctgacg ggctttgctt tttgaccttg tacatttttt taaaaagcga tttttatttc 56100 tttttatatt atgcctgtgt gatgtaattg tcccacatga gtgccacggc cacgggagcc 56160 tgaagagagt gtggcatctt ctggaactag agttacagat ttgtgagccc tgtgtgggtg 56220 ctagaaacca aatccaggtc ttctgcaaga gcagcaagca ctcgtagctg atgagctatc 56280 tctgtagctt ccggataatt attttgatga caaatgtcta cctgagcctc catttataac 56340 attgcaatca ttcctagaga tgtgcactag tagatttgta caatagagtg caaatcagca 56400 tatttcatga agacttaaaa gcttaaggtc cttcagccat ttggaatggg caaactgctg 56460 ggacatttat accatgcagt gttgtatagc agagaaaatg ggtgaatcat cattacatgt 56520 gagtgaaggg ctggagagat ggttcagtgg ttaagaacat gtactgctct tacaggagct 56580 tagtacccag taccatgtca ggcagctcac aactgcctgc agttctagct ccaggcggga 56640 atcgaatacc tctggcctct gagagcaggc atataccacc atacacatat atgcataatt 56700 taaaatgaaa ataagttttg aaaacaatta aaaaataaca ggatgaataa agactgaaca 56760 acatgtttga gtctatgccg gggcctagca aacacagaag tggatgctca cagtcagcta 56820 ttggatggat cacagggccc ccaatggagg agctagagaa agtacccaag gagctaaagg 56880 gagctgcaac cctataggtg gaacaacaat atgaactaac cagtaacccg gagctcttgt 56940 ctctagctgc atatgtatca aaagatggcc tagtcagcca tcagtggaaa gagaggccca 57000 ttggacttgc aaactttata tgccccagta caggggaatg ccaggaccaa aaagggggag 57060 tgggtgggta ggggagtggg ggggtgtgta tggggacttt tgggatagca ttggaaatgt 57120 aaatgaggaa aatacctaat aaaaatatta aaaaagaaaa aaaaaagact gaacaacaaa 57180 aacaaaccag aagttccagg agaatattgg ctccgcactt tatctttttt aaggtccaaa 57240 tgccaaaaca ggtatttagg tagttgttgt ctaagccatt tgttgccgat ttaagtaaaa 57300 gtccatccat tttctgtgcc tgctaaaatt gatactttta aagcaagaaa atgaaacagg 57360 aggaaattca aggtggagga tgtctcggtg gagcaggagg gcgtgaagag agagcacacg 57420 gaagtgaggc tgttgaccgt gttctcactg tgctgggcag ttgagacata gatacttatt 57480 tggaaaagaa ttaaactcga gggactcagt ggaatggaaa aagtcagcta tgggtggaac 57540 accagaccat ttcatgaact gggacttgta aatgttctca ttctggcacc tgctgagctc 57600 ctagtgacat aagagaccta agctccagat ttattccgaa ttaacaaatt gagccagatc 57660 ctcagatctc gtggaagaac aagcaggtgc tctcgactaa tgagccacct ctccagtccc 57720 aaggtataga gctttgatct ccatttttct ttatgtacaa atatttccct aggcattaaa 57780 ggtgtaattt ccataatcta ttcagtgtat caggctgtgg tcatactgaa taagaggttt 57840 tcttcacaga agcatcctgt acattaaata gaaagatcaa ttttaaatga cacctgagct 57900 tactccagtg tctctgctaa tttgttgaac ttttccttca taatctcttt aaattaatta 57960 ttaactagcc caaagcaggg tggacttggg cattatagag tacctggtca atttagaaat 58020 cgtttagtcc aagtcattag aggattatta cccttttttt tttcttcagc cagatggggg 58080 atgtcataaa tgtacacaat tagggttctt taaattaggc ttatggcttg gatgtgaaat 58140 gtcccctaga agctcacatg cttgattact tggtccccag ctggtagcac tctttcagga 58200 ggttggacag acacacagtc tcattcatag ttggtggggc atagggatga aacttgaggg 58260 ttacggatcg cctctgattc tgtcctggtt gctctgtttc cagatccagt cacatgtgag 58320 caagctatct cacactcccg ttgtcataac ggactggttc tgtttccatg tgtttctcat 58380 ggcggtgatc gactttatcc tctgaacctc gtgcaaaccc tccatgactc aagttggtcc 58440 tcctattagg tgtttgccct ctgtcatgag aagagtgaca cccatagtag gctcctttcc 58500 atccatctcg catgtgcccc acggtcaggt tctgttggtc taaggctgac catcaactgc 58560 tgctgaacat atatatatag ttgagaaact tttccagtca tactttccaa gaaataaata 58620 ggagcttgag tgtggtgagc tgtagctctc agctggccac tgatgtaatt attaatcact 58680 gcatgcaatt aattgggacc tttcttagac atgtttgcca ctgatccctg tttccaggga 58740 gtttcacaaa tgttcccacc ccagcctcgt acctctattt gttcttttgt aactttataa 58800 aaattttaat ctatacaagt ttgcaatgca ttgatcatat ctattcccac cctcaccttc 58860 ctggttatcc gagattctcc ccaccatatc cctttgaagt gtcagatctt cctcttctgc 58920 tctttataac ctactaagtg gtgttcatat gggcacaggt gtaggaacat tcaccacagc 58980 atgagcaacc tgtaagagac cacattcctg aagaaaactc ccctttctcc ccaggggaca 59040 tcaacggcca ataactctca gttatggatg gggtaccttc cgtttatcct ggaatgttga 59100 ctggatgaat ctgtgtaagc agccactgct gctgtgagtt cgttaagtgt aatggcctca 59160 tgttgaagtt aagccctgga tcttcctcca agctccgtgc tcccggaaat aaggctcaga 59220 ctcaaaatat atttacaaat accttggcca tgtaactagg ctcttctctg actagctata 59280 acttgaaata accattgatt ctagcctaca ttctcccatg tgattggcga tatgagctca 59340 ggtaccaggc atctgtcttg tcacatcttt tcagtgactc tctctgcctc cttctctccc 59400 agaattcttt ctcctcccac ggttcacctt ctatttcctg cctaagccat agcccatggg 59460 ctttttagct gacaggtgat gcattcatac agtagacgag atgttctgtt tatagccttg 59520 ccatacccag aagacactgt gtcacaacag tcactcccca tcctgacctg cagcccttat 59580 gaccttttgg ccctctcttt cacagtgttc cctggggggt ggtaatggtg gtggtatgca 59640 tatatgtcct gttttgagag caccgcatgg tcattccaca tttgttctta agtggaagaa 59700 agcatgatgc aaaacagaag gagtgagcta gactttaact ggatttaggg gactttataa 59760 gagaaagtaa aaacgtcctc aagaattcat taaaaatgaa gagcatctcc cccatgctca 59820 tttctgttca ttacaaacga ctcaactatt acatgcaaat attactctag gaaaatagcc 59880 acccactgat atgggtgtgg agatctccaa agacaagagg aagttgaaaa gggctcagat 59940 ggctcctcca atgtggcctt ccagcttatg acaagagaaa cacaattgct ggtccaggga 60000 cagatgtctg ctgcacttgc tttgttcctg gctctccctc tgacccaatt tacagccccc 60060 ctgcagctgt ctcctgcata ctttcccacg tgaagttatt tatctttctt aacagtcaac 60120 tcgatttatt ccaagagaat gctcccctcc cccagctctg ctggtaatct attcctgcac 60180 taaattgcca ttgtaatcaa aattcctaga atagtttaag ttggccttct gcagctgcag 60240 tcacctctgt caaagccctg gccaagcctc ctcagtgccc tggagtgagt gtcattccac 60300 attgctggaa accacacccc atctggctct tcccgccttt ccaggctcct tttgctgtgt 60360 ccttttatca tctctactga accactttcc tgacaaagct ccaacacggt tcctcctcta 60420 gagctaccga cctcttcaac tcctatgtgg tcattttttt ttctgtcctt ggccatttct 60480 taacccattt ttttaggaca tcctcttctc tccttgaaac ttggtcagat ctgccaccat 60540 atgtaccact tactcaatat tttgatcccg gagctcagtt ccgtggtggc taattttgtc 60600 aacagacaac atctggaatc atctaaaact caagttgctg ggcacttctg tgaggcagtt 60660 ttttaaaatc agattatttg aagcaggaag acacatccga catccgggcc ccactttctg 60720 atggcagccc agataacatg acgtggaaga aggacacttg gctttttttt tttttttttt 60780 ttgcctgctt gccccactgt gtctggcatc tctcctgttg ctcaggcatt ccttcactat 60840 tcagaatccc aacaaagata gaaaaccatc tgctctccag gaatcctcca ggatcccaga 60900 gctaggtgag gactgctgag acacccagcc ttgtggactg aacagctgcc agtttagata 60960 ttggtctttc catcatgaga ctgccattgt tgaactactc ggaccatatc ctgtaagcca 61020 gccttataaa tgcatacaca cacacacaca catgcacaca cacacatgca cacacacaca 61080 cacacacaca ctcacactca cacatgcacg cacgcacaca cacacatgca cacacactca 61140 caaatgcacg cacgcacaca cacgcacaca cacacacaca catacacaca ttcactctca 61200 gttctgttcc ttcagagaag cctgactaaa tcaggttccc agcacctatg atgggcagtt 61260 cacaatggcc tgctaatcta gctctgggag atctccttgc ttcttctcat ctctgcaggc 61320 actgtactca ttgtgcacac ataaatacta atactaataa taatagtaat gaaaattata 61380 aattcatatg cataccagtt tttattagtt ggcaacagat tgcataacca caacaccact 61440 tcacaaccag aaaattgaca gactatacta gtcatatcca ggtttcatag ttttgccctg 61500 tgcttagaca tgcatatttt cagctattca ttgattgctt gccatactag aaactgacct 61560 acacaggcta agccagtgct ctaccgctga gatgtatccc cagccttctg gttccatctg 61620 acttaagttg cccaaggtag cgttgatccc aatctgtaac acaggcatac ctgaaacttg 61680 caatccttct gcctctgtca cctgcgtagt tgagatttta ggccggtgcc actctgtctg 61740 taactttgtt atgtggagag actcgtgtgc ctctctatga gatacacttg ggaagaaaga 61800 acctgccggt taagggatgg cctccatcag gttgcctatg gggatgtgtg tggtaccttc 61860 tctgtccagt ttactacggg gagcatcttc cctaggcagg tcgcttaggt tgtctaagaa 61920 agctagctga acacggagca aaccactaaa gggcatccct ctgtggtttc tgctttaagg 61980 tcctgctttc aatctgtctc ctgtcagatg aaacaaaccc tttcctctcc aaattgcgtt 62040 tggtaatggt gtttatcata gaaaaagaaa accgagaaaa agaaaaccac catagtcaaa 62100 cttagactcg tcccatcagt ggcctcttgt gatacctttt agaaccaact gccacctgtc 62160 ccttctcccg acctgtgacc agcaataata tgtctccaga tttatacttt gattaatttt 62220 aagaatgttg cataaatttt atcaaacacc atacggaagt ctgaaatttc cctccccagc 62280 atgattctct tgatatccac ctgagttgtt gtgtagaccg acactgttct cattgctatt 62340 ggtgagtagt atttctgaat gtggacttgg tagtttgtct aattgctcgc ttgttgtgag 62400 atgcttgggt tatttctagt tattgaaaat caacatatat gaaaatgcat atgtagattt 62460 tgtgcacttt cacttctctt ggataaatgc cttaggaaaa tgttttctgg gtcatatggc 62520 aggcatatgt ttgatttcct agagacatca atgcagtttt tagactgatt gtcccatttt 62580 acattctcag tagctgcgta tgagtctgac atccttgcca gtgtttgttg atgtccttaa 62640 attttttgaa ccattctgat agtagggtag gatctctatt ttatttatat atgtgagtat 62700 gtgtttgtgt gtgagcatgc gcatttgtgc atgtatgtgt gtatgtgtgt gtgtgtgtgt 62760 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgc atgtgtgtgt ggaagaccat cctcaccctt 62820 ggagctggag ttataggcaa ttgcgaacca catgagatgg gaagtgaact caagtcctct 62880 ggcagagtcc tctgtgcccc taaccactga gtgacccctc aactccttag tgtatcatac 62940 tgtatgttta ctgagtgttt ctatgatggc ttatgctgtt gatcctcttt tcatatacta 63000 ctttgtcatc tttatagcct ctttagtggc agatctgtct tttctaagtt tcctagtcta 63060 atattttaat cactgagttt tgacggttct ttatatgtct tagatagtgc caacctccac 63120 tctgtttaga tgagcagaag gtctatgggg ttaaaaggct ttttcagtca tttagcaacc 63180 gagaaaggaa agatccaagt ctgcccctgt ctttatgcag tctttaccat ggctcatctc 63240 atatgtagtg tcttcccaac tcatcaagct acattcatct tagcatacat agtctcccaa 63300 accaaaaacc cataaccagg gtatacatcc ttttggtggg gaattgtctg aagtttgtct 63360 tctaaagtgt ccagcctgtg ttagtcattg tatcgcccta tatagtcaac aggggtgggg 63420 atggaacaca acacctaagt atccctttag ccccgaagag ccttcagttg tcactcctca 63480 tcaaaactat ttaactagag tcatctcatt caaattcaag tccccttgcc agattaaaat 63540 atagctgatg agtagttatc atgaaggtaa agaagccagt cacacggcta gggagatggt 63600 caatgggtaa agtgcttgac ttgtaaaccc taggacccaa attcatgttc ccagcaacca 63660 cataaagtca aacgtggcat cttgtttctg caagccgagg tgtgaagggt gaagaagtga 63720 atcccaggga cttcgggtgg gtcaatctag ttgaagcgat gagttccaag tttagtgaga 63780 aacaacgtca aaaaataagg aagaaagaaa ttgaggaaga cattgtgata tcatcctctt 63840 tcgtgtacat gcacatgggt aggtaagcac gcaccactca cacacacaca cacacacaca 63900 cacacacaca cacacacatc aattcttcat agctctaagt ggattctttt tgtcaaagaa 63960 tcagctgaag cttaatggag attgcatacc tgagctcttg tcttctacag ggattgtaag 64020 agcaatgccc agatgactct tatgtacaca gctgtcccag agtctgtttt ctaatcactc 64080 agcctgtgaa aactggtgcc atccatgact tgaacaggct ggtggtcttg ggagccagat 64140 cactttccca gtgggcactg aggagctcca cctgccacca gatagcagag tactgattag 64200 gccttgaacc agaaatgacc agaaaagcat gttatagatg agaatagtac agtggatgtc 64260 atgttctagg tgtctgagaa atatggagga aaatgaccct aatcataaag aaattatagg 64320 gtagatatta agcacgccta aattattaac cagacaataa gaggacatga gagattaatt 64380 gaaggttaat agctcattgt gaaaggcagg ggcctctctg gtagcttata aagatgctct 64440 tggcttttgc aacctgaggc ctgagaaatt gtggcctatg acttaatagt aagaatggtg 64500 gaacaacaat gaagggttaa gacctgaagt gaggccatgt cataagttgt tgggccattg 64560 tcctcaccca ggttaggaaa caatggaatt tgacacatat ggcaggagtg gattatgtca 64620 aaaacaataa aactgaattg aattaactgt attggatcta gagttgtttt tttttttttt 64680 ttttttttaa attctgtgct gtaagcttcc agaaaaggca tactccttcc tgttaaacac 64740 cagtgctctt ctgatgtatc aggactgtat tagttacctc tctgtgactg tgagaaaaca 64800 ccatgacaaa ggcaatttgt agaaggacgg gttttactgg gttatagttc cagagagata 64860 agagttcatt atggcaaaaa gagaaggctg gagataggta tgggagctga gcaggaagcg 64920 caaaggccaa tggcaaacat gaagcagaga gaacaaattg gaagttgggt aaagctcaat 64980 gcttgttgtt agtgatgcac ttcctccagg aaagttatag ccccacactc ccatcgcctc 65040 tccaaacaat tctgccaaca ggggaccatg tgttcaaatg cctgtgggag atagttcaca 65100 ttcaaactac cacaaaaacc atacagtgct ccatgagtta ggaaaacatg cctgctttac 65160 cagattctct cctggtcacc aagctaaacc ctaaggatat atcaccacaa cctggcagag 65220 gactgagctt ggtctggtaa tggaagaagg tataaaccaa aaggaacatc aggtcctagc 65280 cattaccata agtgggactg gattgtgtaa gttttgcctc aaaggtggca ttattctaag 65340 ttagatgatg gaggatctgt ttcttgaggc agggtctcct tggatacagg agttaccatc 65400 tatcaagata ggataaagct gtggctggca tgaatagaat catgatagat gatttaaagc 65460 ctatgtgggg gatggaagca gcctccccta aagtaaaagt ggaaatgcat tattgtcatt 65520 gctatggatg ctttctgggc aataaaagac agtgggatat gacatacagc tagaagtctt 65580 atttaataat ttctattatg atgctataat gcaaagcatg gagatacgga cagatacgat 65640 gagtactggt ataattaaga aattcagcag taaatagctc ctgaagtcat ttctaatcag 65700 agatgtcatt gtggagattg tggagattgt ggattgtgac acagatagga agatcccaaa 65760 ttaacagccg caggggttcc atttcaaact tagatgccag gtgacagcat ttaccaaaac 65820 agaaggtcaa gtgcaaaaga acaatcagga gatctgactc caagataaca atggcaattg 65880 tcaacacagc ttcctagcgt tggggagaaa agagatgtca agagacaata aaaccattgc 65940 acgcaccaga gcaagaggag ccaagagtgt atgagcaaga tcctagagac aaggtttctc 66000 cacagtgtgt tctgtcaccc aggttcagag ctggcccagt tttcaaacac agaacccaac 66060 gatgaagtga tcaggtgtcc tggaaaaaaa aaaagatttc ttcaaaacca tggtgactta 66120 acaggtaaca attccctgac acttccctga agcaatcttc aatcaccaac tcagttagcc 66180 ataagttatg ggaatgtttg gttccaaaca tgtcaacctg acacaggatc atacacagat 66240 tcaaagtatc aacatgaacc ttctgttaca ctgggagcac acctggccat gaatggaagc 66300 tcagaacagt ttcaggatta caatgtgtcc acagagtagg taggctcaac cagtgatcct 66360 ctctcctgtc ccagaatgta acattgaaga agtgcacttg gcattgggcc cagcttgggt 66420 tcctttacct gtggatgaaa cttaatgagg gaaacattaa taacattatt aacattaata 66480 acataattaa aacataatga ggggttggac agaagcacca atgagaaccc tgttgctcaa 66540 atctgcccct tacaaaggta taaaatgtgt gttctttctt tgttctgggt ctctcctctg 66600 gcctgcgtgc tgagagggga gtccccaaca tgttggatca ataaaaatcc aataaggcca 66660 cactcctaat agtgccactc cctagcccaa gcatatagaa atcatcacat tccagtctct 66720 gaaccccaga ggcttgctca aacatatggg acataactaa acctaacata atgcaaagtt 66780 catttagtcc aacttccaaa gtcctcatag tctgtggcgg tctcaacaat gttttaactt 66840 aatcttttaa gaccaaagtt caaggtctct actgagattt actcaatcac ttaactgtaa 66900 tctccaaagc aagacaggaa accaaatggg taaactccaa actttggatc ttcatgtctg 66960 atgtcaaaac tctcttcaga tctccaacct cttttttatc tttgttgact gcaacaaact 67020 tctttctctt gggctggttc cactccctgt tagcagattt cctcagcaga tagttcatgg 67080 ctctggcatc tcaaacatct tggggtctcc aaggaaaatt caatgttaca gcttcttgtt 67140 tcaatgtctg ggatccacac ataatcttct gggattctcc aaagggctgg tgtcacttct 67200 ccagcactgc cctcggtggc attctaagtt cagagtgatc cactccactg ttgctgctgt 67260 tcttggtgat catcccatgg tactgtgttg tgtctgctct tgaccagcaa gaaagacaca 67320 acggccagat tcttctcaac agcctttatt gcaggaatgc ctcaatgctg ctacagggac 67380 ctagggaacc cagggaggtc tgcttatata ctctccagca cagggaaagg cttcatggcc 67440 ccctgggatt ggtcagtctg ccggcaccta atttgcatgc acccgctccg gaggggttgg 67500 cgccagactc ggacttacat ctgcgctgat gttgtttatg acaatggcac acctggtgcc 67560 cagagccatc ttagagttgg cttcctacag tactggtatc tccaatactc tggggccttc 67620 tactgcaacg aggcttcacc aataacctct cataggttct ctccatggtg ccaagcctca 67680 acttcttttc atgacccctt cagtcctggg ctgtcaagtg caactgaggc tgcatcttca 67740 ccaatggcct tccatggcct ctcagagtgc caagcctcag ctgctcttta tgttcccttc 67800 atcccttcaa aaccagtaac acctgagtga tggggatgga tgtagatgag gtgaaaccca 67860 gaacacctct cattccctgc aagggaagac ccatgcctgt agccagaaat gttaggatta 67920 gagcaaaacc cttagtagaa actgctgtga ttctcatttg gaagaccatg gactgatctg 67980 tcagcagtgt tattggaggt agcctttttc tttctccatt gtgaagttga tggtccttgg 68040 gcagacttca agaaagggtc tgaggagaca gacccaggaa gagtgtagca ataacatttg 68100 atttgcgatt aagaaggctg gagtcataat tagccctaaa tgggacaact ctatcaccct 68160 ctccaatgct cagggaagag gagacagaaa gaatgtaaaa gccattctgg gcttgacata 68220 gctgttgtct tcatgtggtg acctgcacta gagctgcagg cgactgggca ttcatcaagt 68280 gtgtgggagg gtcccctgag gccccacccc tcccttcagg actaacagaa gttagtgctt 68340 gccaagggag tagctatcat cctctgcagt gacacggaag ctgacaagtt acccatgctc 68400 caggaaataa actcccaccc acgctcaggc aggcgactta ccttagactg agtaagtcac 68460 acacaaaaga agacatgaaa gaaggagggg ctcttcgttg agactaaggg tttcaagtgg 68520 gagaaagaga aggataagac tattaaaatt cattacatac aggtatggaa ctgccaaaga 68580 agaaaatgat gaagagcagc agctgccgcc accgggagtg agtcagaggt ggcctgcaag 68640 cctgcaggaa cctggccctc accaacagaa aaggcggagg acagacacct gacaagagtg 68700 ttctcttccc tccactaagt gtcttttaca gtttacatgt tgatttggga gactgttaat 68760 tctgggatat gcggggggtg gggtgggggg aacaactcca acattctgga ccaaatttaa 68820 agcaaccttt attaaatatt agatattgac caagggatgg accttagttg ggaccatttc 68880 acagaacttc ccacctgaga atggcctgag ccatgggttg caggggcaaa gggcaaacct 68940 ataggccact gtactttccc atgatgcttt gctgtttcct aagaactata attcccagca 69000 ttgcaggaag ttactgggtt tctgggcagg taaggcttac aggttaattt tgggcattat 69060 aagacaaata ccaaaatatt atctatgata ctattgcttt attttatttt attttcaatt 69120 acatatgctt gtatgtgcat gagagtgcaa gtgcccacag aggttagaag gaggtattag 69180 gtatcctgga gatggagtta caggagatta tgagctatca tatagatgtt ggaattaaac 69240 ctcagtcctc tggaagagca gccaatgagc catatctcca gccctatcaa cctgttgggt 69300 tgtttaaaaa taattctttt atttttattt ttaagtatgt gtgtgtttgt gtgtgtgtgt 69360 gtacatgggt gcagtaccct aagagaccgg aagagaacat tggattcccc ccccccccac 69420 cgcccccacc tcccgagctg gagttacagg cagatgtgtc ccttggaaac caaactcagc 69480 tcgcctagaa gaggactgga ctatcgagcc taagcgccca tctaagtctt tgtatccagt 69540 ctttctgcaa ccctggaagc ctttttacca aaaagtacat tctttcatcc tagctgtttt 69600 ttgtgactag cacctgctga aaaaaaaaaa taagaaagaa agaaagaaag aaaaaaccca 69660 aaacatcatc aaaacaacaa aaagccaagc ccaccaacaa gaaacggata tacacaaaag 69720 ccaagaaaaa caaaacaagc ccccccaagc cccagctatt catcaagata ggaaagagaa 69780 acacccaact cctacctgcg cttggtgatt gcttggtgaa aaataaaagt atttttcctc 69840 tcattccttc ttttgtgatc aaatgaataa tgaagggatt gagcctttgc tagtgtgcat 69900 gcccctgcag ggagccaaga aacaaatagg tttcctaagg gtataaccca gccttttcgg 69960 cttttccgtc atcacgtgat tgagggcttc tgcttcccac ttaacctgca tccttcccag 70020 actgattcat ggaacctcaa gggctctaca ggaaaattca aacatcagct tctctctgcc 70080 ataggacttc catccgaagg aatggttcct tggttgttgc cttcacagcc ctcacatccg 70140 ggctggacct ggcagaaagt tgacatttga cccctatcta gagcatagac gatgctggca 70200 gccgtatgtc ctcacttttg ccattccggt gcctccctcg gggcacagcc aaggttccga 70260 gatgggcatt ttcaggagga aacgatttta tttgaggtct tggcagggag atgagaggag 70320 gaggcgtgcc accttctgta acaaaaggca tttcccagcc ttagacacac ccttaggccc 70380 agatgtacac tcccactcct gtcaatccaa ctgcactgac tagcaaccct aatgcctgct 70440 gcttccaggc accccgcagc ctgcgtcttg tttccactat ttcctctgcc tgtcgcaggg 70500 aaagcctcac taccaagagc cttcaaacac agttcctgtt actaattttg ccccaaaggg 70560 taccacagtg attaacttaa tgcttcccca gggatgactc tctctgtctg gggtcagggt 70620 ggggctggat ggattttctt tattaaggat ggtgggaacc ctcttttgag ctttgaagtc 70680 tcgccttaga ttttttttta attttatttt ttatttttga taactacaaa gggacaggaa 70740 gtctcttttt actcctctga ggcttgctgt tctccagggg catggaaaat gtacacgttg 70800 ttagcccccc aagcctgggg gctggggccc gcgctctgcc ggattgtttt tatatcccag 70860 caaaaagcat ctgtgtgcag aacagcagct ggtctgctgc tgccaactgc acgttcccag 70920 taccaatgta agcactttcc ttagaggtta aaacccccaa ggcactgttt gtgtttggag 70980 aagaaatgct ctaaaaatag taagtggggg tttaaagtct cttcgtcaga tgttcggaag 71040 cacgctgacc tggtgcgctg cttcttagaa ccagagctca ttggcaggtt gctgtgcctt 71100 gcacctacga ttcctgactc cttcagtaag ctagctggtg aagagctgga gaatctgtat 71160 ttataataaa tccccgggtg gggcattgat gttgctgatt ttaagaacac acactgttgt 71220 agccagtcaa aagaccctta ctcagaaaga ccacagagac cgccgccatc gctgcaaacc 71280 gcatgaggat ttttattgat ccaacatgtt ggggacaccc ctctcagcac gcaggccaga 71340 ggagagaccc agaacaaaga aagatcacat tttttatacc tttgtaaggg gcagatttga 71400 gtaacagggt tcttattggt gtagcaagta accctttcag gcattggttg gtttgtatag 71460 tgactaagta accctatggc ccagtgacta ctttgcttgc ccccatggtg ggttattatg 71520 atttggtcag caaagtaata gtactttaac atcacagcta ttaacgtcaa gggaattctc 71580 acaacaagct cagggtggtt tgtggtcttt gtcagaattc agtgtggggc aggggtcagg 71640 agaattgcta atcttgttat ggttacttct ctgagaacag ctagtattgt tttggtagtt 71700 gcaagcttaa tcattttgac caccaaaact atgtttttac atttgtttag tcagccggct 71760 tccctatctg gctctgcacg tggcctgcaa gtacagtttg gaaagtgttt ttcgaaggag 71820 gaaggtactg ggtggtcttg aattcatttt ggatattaca acacaaagaa ccactggtct 71880 ggtgtatttc cccccagtct gttgttggct gcagggtgta atacagacac agggtttatt 71940 accagggaga ggagggtacc atagcttgat gcaattcctt attacctgtg tcgccaagag 72000 caagtagctt aaccttgctg gtctctgttt cctcccttcc ttgacaatca tttctataga 72060 gctaatttat atcaggatct taattatctt gttttgtgga gggcacatgt ttataaaacg 72120 ttgatctttc tccctaccaa tattttttct cattgtcatt taaagagtca gttttgggac 72180 tgacaagatg gcttagtggg tagagaaggc ttgccaccaa ccctgatggt gggagctcaa 72240 tctttgggac tcacaaggaa gaaggagaaa acagactctg atctgtacac atttaaattg 72300 gcacgtgagc ccttctcctc aaataaagtc acacacacac acacacacac acacacacac 72360 acacagagag agagagagag agagagagag agagagagag agagagagag agagagagag 72420 aaagatacac acacacatcc catacacatt ttttggccag gttagacaat gctggggaaa 72480 gatatcagat ttatgcatct ctctctctct ggactgagtt ggtgactgtg ggaagggatg 72540 ggacttgact actgcaactt gtcagagcac agtaggccac tgtgaattgc agctctcaga 72600 ctcctggtac catcccatag tccagcacat gggagctaaa agtccaaata acaacaatgc 72660 tgtctgagga cttggaggca ggtgacattg tggtagggat ttgtggattt cgatgtgtgt 72720 ttgttctctg tttttaacac cttccatcag ataagttcct gtcctgtttc ttcagatccc 72780 aacctgtttt ccatttcctt ggggagcctt tgaaatatgt ggctggatct ggatgttatg 72840 aacttgtatg gagttctgca caagtcttta tagtttcatt tgattgcaat ccaattaatg 72900 taaggatcct ccctgaatga tgcgccctgt gaaagcaaac tccatgctta atctcttccc 72960 tatacttgtg tagtatgcat ttcttgactt ttcatgagac attcagagac tatcctataa 73020 aagtgtgtaa gtcataaaag tgtgagatta aaaaaaatag caggtgcaaa tgaatcacat 73080 tttcttgatc cattctttgg gaaacatctg ggttgtttcc agtttcttgc tattacaaat 73140 atagctgcta tgtacctggt agaacaagta tccctgtggt atggtggagc atcttttggg 73200 tacacgccca ggagcggtat agctgggtct tgaggtagaa ctattcccaa ttttttgaga 73260 aatcaccaag ctgatttcca gagtcgttgt actgttgtgg ccggccagca gctcgcaacg 73320 tgaacgtgac tgagatggcc actcgagcta agggaggaac tagtcgggga gaaagaagaa 73380 acggagccaa gacaagttca tcctgttcaa agctcaattt tactagttct gacattcagt 73440 tataaaggaa gggggaggga acccgattcc cgccaactgg tcttggagtc cagtagcagg 73500 gtgaacacgt gtgtggctcc aagcagcaaa gcagcacggt ccagcagtgg gcgtggcaga 73560 atgaatgagc gggaaactcc acccctgagc cagcaggttc caggctgggg gtggggaggc 73620 tgcagttgta caagtttgca cctgcaatgg aggaatgttc tcttcgctcc acatcctcac 73680 cagcatttgc taactcttga gtttttgatc ttagccattc tgatagatgt aaggtggaat 73740 ctcagagtca ttttatttgt attttccaga tgattaagga cattgaacat ttctttaaat 73800 gttcctctga cattaaagat tcttctgttg agaattctct gtttagctct gtactctatt 73860 ttttaagatt ggattatttg ttttcttggt gtttaagttc ttgagttctt tatttatcag 73920 tcctctgtca gatgtagggt tgttgaaggt cttttcccat catgtaggct gcctttttct 73980 cctattaatt atgtcccttg cattatagaa ggttttcagt gtcatgaggt ccaatttatt 74040 aactgttgat cttagcgcct gaaccatttg tgttctgttc agaaagttct cttctgtatc 74100 agtgcattca aggctatccc cactttctct tctattagtt ttagagtatc cagttttatg 74160 ctgaggtctt tgatccactt gcacttgagt tttgtgcagg atgataagta tggatctatt 74220 tgcaatcttc ctcatgcaga catccagtag gaccagcaca atttgttgaa gatgctttct 74280 tttttccatt gtatggattt ggcttctttg tcaaaaatca agtgtctata catgtgtgag 74340 tttatttctc tgtcttcgat tcaattacat tgctcagtta ttaaaagcaa ggacatcatg 74400 aagttttcag gcaaatggat ggaactagaa aatatcatcc tgaatgaggt aacccagctc 74460 caaaattata agtagatatt agccataagt atacatacag gatacccaag ctataaacca 74520 gaaacccaat tatgctaaat aacaaggagg gctcaaggga ggatgcttaa tctcactgag 74580 aaagtgtcct agtcagggtt tctattcttg cacaaaacat catgaccaag aaacaagttg 74640 gggatgaaag ggctttttca agctacacct ccacattgtt gttcatcacc aaaggaagtc 74700 aggactggaa ctcacacagg gcagaaacat ggaggcagga gctgatatag atgccatgaa 74760 ggggtgctgc ttactggatt gctcccctag cttgctcatc ttcccttctt atagaagtca 74820 ggaccaccag cccagggatg gcatcaccca caatgagccc tcctcgcttg atcactaatt 74880 gagaagatgc cttacagctg ggtctcatgg aggtatttct tcctcaaggg aggctccttt 74940 ctctgtgata acttcagctt gtgtcaagtt ggcacataaa accagccagt aaaggaggag 75000 aaacaaaata gtcattggag gggatggagg gaaggaactg gttgagagag gggctgggga 75060 gggaaatggg gagttggtca tcaggtgtag ggagagaagg ggaaaggggg ctgtgagagt 75120 gaatggaaat ttgtggcagg tgggagtggg gtgggggtgg ggaggctgcc atatcatttc 75180 taggatgtgt cagagacctg ggacagggga ggctccaggt agactatgag gggcctctag 75240 ctgagattcc ttgtaatggg ggatatggag tctgaaatgg tcacttcctg tggccagaca 75300 ggacctccca gtagagggaa aaggatacca acccacccac aaaacctttg accctaaatt 75360 tgtcctgcct acaagaagtt ccgggacaaa gatggaacag agacaaaggg aacgaccaac 75420 caatgaccag cccaacttga gacccgtgcc gtgggcagga actaatcctt aagatttaat 75480 gatactcttg ttatgcttgc agacaagagc ctggcatatc tgtcctctga gaggctccac 75540 ctagcagctg actgaaacag atgcagagac tcatagccaa acattagacg gaacttggaa 75600 aatgtggaag agttgaggga aagattgagg gacacagaga ggataggaac tccacacgaa 75660 caacagagtc aactaacctg gactcttgag agcttccaga gactgaacca ccaaccgagc 75720 atgggctgga ccttggcatc ctgcatttat gtagcagata tgcaccttgg tcttcatgag 75780 agtccctcaa caactagagc agggactgtc cttgaccctg ttaactgcct gcctgtggat 75840 cctattccaa ctgggctgtc ttgtctggcc tcagtggaag aggatgtgcc tagtcctgca 75900 atgatttgat gtgccagggt gggttggtac ccagcacccc cctcaaagga gaagaggatg 75960 agggaggagc tatgtgatgg gggtactggg aagagaggag agatgcaaat tggaagtaaa 76020 gtgaataaat aaataaatta attaatggaa aaaagtagtg ggtgctatga aggagtcatc 76080 tatattagat tttctggagt tataacaaaa tacttgagag aaatggagtt attttattat 76140 cgttttggag ataaaagtcc aaataacagt agtattatct gtggtaagaa ctactgtaga 76200 tggcactgtg ataagaattt gtcagttcct atgtctgtaa atgtgtagcg tgtgtgtgtg 76260 tgtgtgtgtg tgtgtgtgtg tgtctgcatg tgcatgtatg tggggtgtct atgtttgaga 76320 tggatatgga tgtgctgtgt gttatatgta tgtgtgatat atgtgctgtg tatgtggaag 76380 ttatgtgtgg gtatgggtgg gggctatgtg tgaaagaaag agagaaacag gatagggaaa 76440 ggaagtcaga gccagaccta tatggggaca gtaatgacct ccttatttag atttatctct 76500 taagggttaa agatgacatt attttttaac attgttgctc caggaccagg ctcctaatgc 76560 atgcagtctg agagacacat tcaaatcata tcctatacac agtaccatcc tttggccaga 76620 atattggtaa aaccatccaa atgttagagg agagtagcca acagttctgg catccgtata 76680 cagcactgcc ggcgtagagt gccctactgc cctgaagatg ccctgtgatt tcattgaaaa 76740 ttctttccag attggtaacc tctcagcccg gctctctgtg ccttatagat catgagatct 76800 gtaacataca acgtaacaat tagcttctca gttcttgttg gagaggctgg gtttataaga 76860 aagaaagcag ccaagcacat cagcccacgg gagcctggca taaagtgtac ctcagcagtt 76920 ccttagtgtt tgtagaataa agtaatgtat tatagctttc ctatagatcc acatggaagc 76980 ctgagaagct atgtggttct cctcagacct catagtctgc tcagattcac tcacttgggc 77040 gggttgtata gctctgggga agatttcact tctctgcctc agcaacacag gctcaggcgc 77100 atcctgcttt gggttgtgaa gttgaccgga agtgacacgt atagctttca gaaagaagtt 77160 tgaagagcca gcatgtgact gaccattcgt gcccttatac cttgtttttt ccaaggatta 77220 tgatagagcc acaggccatt gggtgctgat atttgttttc atggtgtaac taaatctgtc 77280 cgcagagcaa cacttctttt ttatgaagat aagaagtaac tgaggcaggt ttccaatcta 77340 gacatggtta ctgtagggtc tgctacttgc tagcttcacc cagctgaatc aagaagagaa 77400 aacaactggc caagcttgtt gggggcatac atgaaagtct agtacttagg aggtggaggc 77460 aggaagataa ggagtggaag gtcagtcttg ggtgcatttg tgtgtgtgtg tgtgtgtgtg 77520 tgtgtgagag agagagagag agagagagag agacagagac agagacagag acagagacac 77580 agagagagac agagagagac acagagagag agagacagag agacacagag agagagagac 77640 agagagagag acacacacac acacacacag agagagagag agagagagag agagagagag 77700 agagagagag agatattccc tggaatcagg ctcccattac aagcactaac aattccatct 77760 gatgccttgc aaattgtttc agaggcctgg gaaagatagg aaaattttct gcctattttt 77820 agaaagccaa tgtaacattg taccaaaatc ttataaagat agcattgaaa aaaaagaaaa 77880 ccaacatccg atctcattga aggagtaaca aatcctaaat tattaaaata ataacagttc 77940 agcaaagcat taaaatatac caagtgaagt ccccttttga caatcaacaa cggcacctta 78000 ccccacacac attatgactg actgtgtacc cctctaaaat aggacgaagg gggaaagaat 78060 gcattgcttc agaaaatgac ccagcaacat atgctagtta tctatttatt tttcttgcct 78120 tgacagaagc tagctagtat tatctggaag cagggaacaa tgtctgttga gaacctgcct 78180 ctgtccggat tgaactgtca caaggcaagt ctgtgaaggc attttcttgg tttaataatt 78240 gatgtggtag gtccagccct ctgtaggtgg taccacactg agtgtgtggt cctgggtcgc 78300 agggttaagg aacctgaaca tgagcctgga gaggaagcca ggaagcagca ctccttcatg 78360 gcctctgctc cacttcctgc tttgagtttc tagcttgaat tctcttcatg aaaaattatt 78420 ggctgggctg gcgagatggc tcagtgggtg agaacactga ctgctcttcc aaaggtcctg 78480 agttcaaatc ccagcaacca catggcggct cacaatcata tgtaatgaga ttggacacgc 78540 tcttctggtg tgtctgaaga cagcagcagc atacttattt atactaataa ataagtcttt 78600 gggccggagc gagcagaggt cctaaattca attctcaaca ccagatgaag gcttacaact 78660 aactatctgt acagctacag tgtgtaatca tatacataaa ataaataaat aaatctttta 78720 aaaattgaaa aattatttgg tgtaaggtaa agaaacaaaa acccaaacac cctttccttt 78780 tcaagtcgct tttagtcgtg gtgtttatca caggagctgg aggccatgta agatgcgtag 78840 gatgtagcat ttagaagcca tgtaggacac ctcgtttagt ggagctaagc cactagtgtg 78900 atataagagt ccgacgttaa tagacggctt taccaaggaa taatgaattc tgtatttcaa 78960 acggagtcgg gattggctta gggttaaaaa gaacctagaa gtcaaggttc cccaggctgg 79020 ggtggctctg atagacgagt gccaagcaca gctgcagata gcgcaaaagt atgacttaaa 79080 agatacatgc tataaggaag aaaagtgagg gaaaattgca aaatatgaaa atagcaagaa 79140 atttcatgta tgtatcaagt ttcccaaaca agggacaggc acacagtcac acagacacat 79200 agacacatag acacacaggc acacagacac acagacagga taggatatta agcactgcat 79260 tttgttcctc ttgttgaaac cctatgtagt ttcattatgt aatccaggct gtcctccgac 79320 tcatgctact ccggactcag tttcccaagt gctggggttt caggcacact gctaccttgt 79380 cccactgaaa gagttgtgcc tgagatggtc attacttagt gaaggacagt gagacttcag 79440 caatggagat ggcatccttg ttagtggatt tgtccactta aatgcattag acaaagtctg 79500 gcttatcatt tattattatt gttattatta ttattattat tattattatt attattatta 79560 ttttaagcat agagttagtg attactcatt cctatacttt cctattgtta aggaaaaaaa 79620 tctaaaatgg caaaatcaca atgtgaaata catatgcaaa gatcttaaaa ccaccagtcc 79680 gtgagtaaac cagttccttt tggagacaaa ggaataggca gtaaataaga gaaaaccaca 79740 tttgttaatg gatgggtagt tgttcactct agccatggag acactgaatc agcactcgac 79800 acgattcctt tacatactta cagaggacat tgattcgttt ctattgttac tgagtagctc 79860 caacttccca ggtcacaagt tgccaagagc agccaattat tcttaagttt caatttgttg 79920 aaaaaaaaaa aagccctgat agcattgatg ttcatccccg agcaactcag taactcagtg 79980 tgtgtgtgtg tgtgtggggg ggggtgtgtg tgtgtgggag ggtatgtctg tctgtgtgtc 80040 tgtgtgcata tgtggacata catatgccat gtcacatatg tggaaatcag aggacaacct 80100 gcaggagtta gttctgttct tgaaccatgt ggggttctgg gaattcgagt caagccatta 80160 ggtttggtgt caagagcttt ttcccgttga gccattttag cccatagaaa ctggttctac 80220 cacaaacatt tacaagatac aaaagctaag gacactgctt agtccattta aaatgatata 80280 ggtcccaaag cttcgcttac tgtgggattt tctcctatca tctcatccct ttctttcatc 80340 tggtctttag gatccttttc tgaaggtgtg aagttggtag agaagcctac tgtgtggaca 80400 ggaatgtctc tcaaatgcat caccattttg agtagtagtg attgagaaca aagggaattg 80460 ttgtaaggtg tggagttatt tgataaggtg tggttgtaac aaagcaccaa aactgggcca 80520 gggagatagc ttatcaggaa aagcacttgg tacagcctcc tgaatgccag agttggatcc 80580 ctggagtcca cagaaaaagc cgagtgtgga aagccagatg cagtgacagg aatttgtaac 80640 ttgggcattc ctgggaagag atgaaaggag aagatgggag aatggcctga agcttaaggg 80700 acagctatgg gatgtgtgct acaagaacag aaactaggga gactctgcct caaagtgggg 80760 tgggaaccaa ctacacacag gtatgttcaa gcagacacca cacacacaca cacacacaca 80820 cacacacaca cacacacaca cacacacaca cacacacatt attaaagctg ataacagctt 80880 ttagactaag tagaaggaaa aagagataac tgtagttaac aaaatctaat tcccaatttg 80940 ctacctacat tccctacctg cagtatgcta actttaagag ggaaaaagtc ctccaaccag 81000 agtgtatata aaatatgttg tctgatcctc ccaatagtgg ttttctaaaa ggaatatcat 81060 agaagatatt tagaagactt ccttttacgt tactttttga gacagggttt tatgtaagcc 81120 aagttgacct caaactttga atcccacttc tctacttcct gagagctaca gctataaata 81180 tgcaccatca tggctggttt acatggtgct ggggacagaa cccaggactt tgtgatgcta 81240 ggcaagttct ctaccaactg aggtcgatcc ccagccctca gataacattg tgtggcgcta 81300 ttctgtctac ccaagagagt ttatagttta gacttccaga tcaagcgaaa taaagagttc 81360 tggggagatg tccttttgct tcctagtgta ggtatagatg ttacgggatg gcaggattta 81420 gtcagaggaa gagactcaac ttctctgaag gtcttactat gcatacaact ttctgctatg 81480 gttcttatcc tgtggcataa ggacatcccc tgccacccaa ttttacatgc cacatcccaa 81540 atcattgtaa cgaggcttca catggcaacc agcagttgac tgcaatgaga cttccatggc 81600 aaccagcagt tgactgtaac aaggcttcca tggcaaccag tagttgactg caacaaggct 81660 tccatggcaa ccagcagttg acacctgcct tgctctggtt ttgttacagc tgaactgttt 81720 ctcatctgtg aaaccccttt ttaaaacatg tattcattta tcttatatgt gtacactgta 81780 gttatcttca gacacaccag aagaggacat cagatcccat tacagatggc tgtgagccac 81840 catgtggttg ctgggaattg aactcagaac ctctggaaga gcagccagtg ctcttaactg 81900 ttgagtcatc tctctggcac ccccgctccc atgaaaccct tttgcatata tctttttgtc 81960 ttctagtctt gtggtccaca agccctacaa gtcacttttg gagttcttcc ttagccccac 82020 tcccacattc ttgctttgga ttatcttcga cattcagtgc tctcagggca gctgtgaacc 82080 tccttgaaaa attttctctc ttgtctggtc tctaatgatg tgtagacttg acttactagg 82140 ggttcatgtc ttgctctctc tttcatgcag ttcaattcat atttagcatc ctaagtcagt 82200 gactataggg aaaggttttg agcccccttg gaagtctctg ctgcaagcat agatcttgca 82260 gagtagatat ttaatgaact cttattaaat ccatgcatga atgaaactct tcatgtgtat 82320 ttcccaatct tctctctaca tcattgaaag gggaataaca gaatttaaat tcttcacaat 82380 tcctgcaaag ggagacttct actaagtatg aaatgtgcaa ggcttgaatt tgtaatgaat 82440 gtctgatgta acccagcatt cccagaatca ccacttgggt tttccacaag ggtatgcatt 82500 cctcttttat acaaatgcat ggagggggag gggttacagg ataggcagaa atgaaagata 82560 tttgttaatt ttctgttatt gtgataaaac actatgatca aggaaactaa tcgaagaaaa 82620 aagtttatgt ggggttatgg tggccgagga ttagagtcca tagcttgcaa tggaggttgc 82680 aggtaaccag aacagaacag gctcacatcc ctgtcttcaa gcaggaaaca gaaagcatgc 82740 ggagtggtgt aaggcattaa acctcaaagg ccatcccaca ggtcacactt cctatacctc 82800 cccaaacagt gccaccaatt gggaaccaaa catcagagtc tgtggaggac atattcgttc 82860 aaaccaccac cacagacaag tagtttattc tgtgcaccct tcttatggag gaggctgaca 82920 agtaccactg tttacacaca gtaggactgc cacgaacaat atagccatac tcactacctg 82980 ctttcccggt ttgtggcctt cccatacctc acagaacttt cttcttcctg cctgtacatt 83040 tcactgctgt ctcttgctgt ggagtggggc tctatatgtg cgagggtctc agtgtgaagc 83100 aggctctgtg ctacttgtct tatcctgagt tacagagatt attttttgtc ccagtcccta 83160 tcaggagtca ctattttgag cccaatctgg tagctctggt ttcttctttc cagaatattc 83220 tgagtcgcgc agcctcaaac cggctctgcc tttcctctac atgcctgtga tgacgactgc 83280 ctggctgcag atgctccggg tgttttccca caccagtttc tcttgttgtg actccatcaa 83340 acccaggtca gcttctgtaa tcgagacgat ccacttaatt aactgtaatg aggctcccat 83400 catttccaat aaatttcttt ccaaaacacc acttgagagg cacatagctt cctatcagct 83460 gtaaattttt tttttcctgt ctatttagtt ccaggaattg aatttactca gagatgtcat 83520 tgcccaacct catgtctcaa tgttttaaac taaatctttc agtctggtgc tatgggctac 83580 aggacccaac cccagtttcc cttgtacaga atggaagatg ccgtgcaaac agcttgtctg 83640 ggtttcacca gagctgggag aatcagtgct ggttttcatg gcctcttctg cgatcagccc 83700 cttctccacc aacagttttg ttagttagac agagcaactt gcgcttgaag atctcaaacc 83760 atttatcgaa gcatgccatc ttctcctttt taaagacctg aaatgctgtt ggaataattt 83820 tgctgtgttt tataaaaata tagagaggat taatacgttt ggtagaggtg caataaggtg 83880 tggagggaag gggtgcctcc aaaggcctgt gctgaggcac ctcttccccc tgagcgacca 83940 gccaaagaat ggtatagtat agaatagagt ttattcatgg catggggagg ggagttgaga 84000 gagtagtaga gacagagaaa gagagaggga gagagagaaa gagagagaga ggggggggag 84060 ggagggagga agggagagag agagagagag agagagggag ggagagagag agagaagagt 84120 agtagaggct ggtcatgagc atgtggagag agagggggag gggaatgggg aatgggggag 84180 caggagggag aggacagagt cggagcaaga aagcaagaga gaacagaggg gctaagcagt 84240 cccccttttt tcattttaaa aattttatta gatattttct ttttttacat ttcaaatgct 84300 tcctataccc tcccctcgcc ctgctcccct acccacccat agcagcccct tttatagtga 84360 gtcaggcaca cctggctgtt gccaggtaac tggggcagag cctatacaaa aatgctaaca 84420 gatttgtcag tgaaatgata ttttgctctt tgacttatag aagatgaaaa agaaaaccac 84480 aagtttcata ctgacccttt ggggaggata catctttaag aaattatggg agagaatagt 84540 ttcatttaga aagaatttaa gtgttttttt ttcccctctc agacatgtca tggtttaata 84600 tcagttatcc aatttttctt tgtgctactc tcagaattct tcctgagaaa ttagctttaa 84660 tcttctgctt tgtttcaaag cctgcactag attaagcctc gtcctctctc tctctcctct 84720 ctctttctct ttggcttcat tcgggaatga ctaattagcc aggagaaatt aaatttacaa 84780 attaaacctc tctgcataga ttttctcaag accccagccc tgggggtaat atccatggcc 84840 tctcctacag agcttttgcc tcccagaggg ttgtatagag gggtgaaatg tttatgaagc 84900 cacatcggct tgaagagcag gttggtggtt ggcggttggc agcattataa gttgttcttt 84960 ggttccttca gggaaaaact tcagtgttcc aaagttcagg gtcatggaag gagggtgagg 85020 aatgttccca gccagagaag aaagtgatgt gtgtgagaga gaactgccga gaactgtaga 85080 actgaagttt ccaccgctcc aattacatgc ctgaatgcct tcttttcatt tcatctctgt 85140 ttattatctt tgtctgtgtg tggatggtgt atatatggta tgtgcattta tgtgtgtgag 85200 tgtacactta tgcatataaa ttcaagtgct catgttttac tccacgtata aagagatcag 85260 aggacagcct tgggtattgt tcattgcctt tcacctttct ctttctctct cttgaataca 85320 ccacctatca ttgtaggaga ctggcatcac agacctgcca tggctggctt tgtatgggat 85380 gcagggatgt gaatccaggt cctgatgtct atgtggcata gacacttaac taggacccca 85440 acccattcac ctcccaagcc tgactctgct cccctgctcc actctacaac atgccctcta 85500 ctcgataaag gcactaaaat gtgtgactag gggccatcca gtttgttctg agtgtcagct 85560 catgcctcct tcttaggaag catttcaagc caatgtctct taataccacc cacggttcat 85620 tgttttctgg cttctccttc cttccttcct tccttccttc cttccttcct tccttccttc 85680 cttccttcct tcctcccttc ctcccttcct cccttcctcc cttcctccct tcctcccttc 85740 ctccctttct cccttccttg ctttttcttt ctaacatctt ttgtgtattg aaaatgctga 85800 tgtttttgct atttggggat gagctcttgc aggatagtgt tccccaactc ttctcttcca 85860 agtttattct ccagatcttt gcattaagtt aggcaggctc ctttagggac agagaccgac 85920 ggcttactgg aggggaaggc ttcagtccca ttaaaagatg gttaatgaac attcaaagtt 85980 agtgtttctc tttgtttcag ctgcagatct tttggagaat cttaggagaa agcagccaag 86040 accactgaat ccacctgacc tcattaacct ggagcaaact gggcttatta gaatttagat 86100 tgaaggcctc agaggttaga tattgctctt ggagaagacc taggttcagt tcctagcacc 86160 cacataggtc ggattacaat tgctgataac tccagttcca ggggatctgt cactttcttc 86220 tgaccttcca ggtcttcatg cacattgtgc acatacagac aagcacacgc acacacacac 86280 acacacacac acacacacac acacacacac acaaatatta aaactctgga gtgtgaactt 86340 agtatgccca taggtaatta tgtccattag ctttcctcat tggacatttt ctctgtcacc 86400 tttaactcag gatgctcaca aatatcccta cccctctacg tacttccttt atgaatttgt 86460 atatgcccct tcccttgttt ggggagcaga tgtttctcta ctctttgatc tgactgttgt 86520 gctcactggt ttcatactca ccaagagacc tgattcgact taggtaccga gtcacccacg 86580 tctctcaggg tcagcctgct ctgtctctca gtttttggtc taagaaaaca aggccttcca 86640 cccttttgat cagcagatat gagtaaaaga aataaaagtt gatgctcttt gttttccagg 86700 gattcagata aaatgtttgc catatgcatg aaataatttt taagaggtag gttggttctt 86760 ccccctaaag caaggtttat ctaagaagag tgcttttaag gatggtcgtg atgctgacag 86820 tacctcgcat tcctaccgtc atttctggaa aagccatttt aatttttcat ctcacctcgc 86880 catctgggag acaagtgaac tcaatgtttt ccttgccatt tataaagtga gttagttaag 86940 actcgggtaa gactattgat ctgcaccaag atccaatacc aggaaaagag atgaaattgg 87000 tatccaaact tttgtttcag gactctgagc cctggcctct attgtctcaa acacagttca 87060 atcataaact gagtcaatac atctagcact gcctggcagt ataaggcaat taaactatca 87120 caaggctatg ggggtaaaga cactatcaca aaggtctggg ataaataaac ttgaacaggt 87180 tgataaagtg tgggactcaa tctctaaagg aggtgctgta atctctctgt agtggaaggt 87240 ttacctgaag gaacagattt gaagttattc ctgaaagcat actaaaacca gaagacagtt 87300 tggcagtccg aatcaaaaac tttaactaaa gacatgaacc acagtacttt ccagcatatc 87360 actcagtggc tctaaagttc ttttccttgt ttgaaaacaa tagagtccag gggaggatta 87420 aatggtgatt tgtaaagtgc caggctcagg gcacattcag taaatgttac cagtgattgc 87480 agaataatgg tgataatatt ttcctttgta ggaggaaata atgagcacgt agatttagca 87540 acacacagat cgtttgttgt gcttttaaaa gtcacagtga aaagttggaa acaacttgat 87600 gtccagcaag agggaattgc agaagagagt ttcctggtat ttctaggtgc tagatcatta 87660 gatagccatg taaaagaata aaaggaaata aatatgttat tgcctagcaa gatggccatg 87720 aatttgttgt tgttgttgtt attaagacag ggtttctctc tgtagccctg gctgacctag 87780 atctatagac caggctggcc tcaaactcaa agatcgatct gcatctgcct cctgagtgct 87840 ggggttaaag gcttccacca ctgctgcctg gctataaatg tttaaaatat attttctatt 87900 aattcttcac aaattccata tatgtgtaca gtgtattttg accatattca cccctactcc 87960 tcttcccaac ccctttcagc tcccctgctg ccatatttcc atcccttacc aattttgtgt 88020 actatttaat taatagatat atacatatat atatttaatt ctgatatatc agcacattat 88080 atattatatt atattatatg ttacatatat tatctgttac ataatataca tgtatacatg 88140 tatgtatata tatatatata tatatatata tatatatata tatatatata tacatattga 88200 tgtacaccca ttcattggag tctggatgcc ttgccaggga ccactcttaa gcaaagtggc 88260 ctctccctga tctggaagtc ccttgactgt taacagatcc ttagctaggg ctggagactt 88320 gggagcccca tgcctctcta agcaggaatc ctgacttggt cttgggctgc cactgtgagc 88380 tcatgaggca gtgttggggg agggatgctt tataccatcc cctttgtgac tgagaattcc 88440 acaaatgctc attctctgca cttggcagtg attttttacc tttcaaaaac acttataaaa 88500 tattaaatcc ttttttttta agtttgtgct tttttgatgt tgtttaacac atatattcat 88560 gtacagatac aggatagctt taaaaactag gagatgaatt tgcagctcta tgtcaacaaa 88620 actgacattt aaaaattgtt tttattaaaa ttctttttat ttaaactatc ccagtaacaa 88680 aggcaagtat catgtaaaat aaaattgtgc tgttttgttt tgtttctggc tttcttgttt 88740 cctactcttt cctttggttt gtcagctctg tgctaccata agcagacacg atccattcaa 88800 tagttgacta acagcattct tttgagtgtt tactgtggcc aaggccctgg ttctaagcac 88860 ttgatgtaca gataaaccag atgaacaaca atgcccttca aaagcttaaa ttcaaggctg 88920 gagaaatggc tcagtggtta agagcactgt ctgctcttcc agaggaccca ggtttgactc 88980 ccagctccca catggcagct tataaccatc tacagctcca gtcctgggag gtctgacact 89040 ctggtaccca cgtggtacat ataaatatat gcaagcgaga catgtgcaca tataattgaa 89100 atataggtat agtgatcttg ggattccagt gtctgaagca ggagaaggat gagactgaga 89160 tcagcctgga ataagatcct cttttgatta aaacaaaagc atgtactagg tgacatgaac 89220 agcacaaccg caagagaagg agagtagacc aaaatttcta gaggagctat gccttcttaa 89280 cagcattcaa cccctcttct gagaatccct gcagactgcc ctccagcatc actgatttcc 89340 atagaggctg catagctgtc aaagttcaca gtctactcag aggtaacctg ctgtgtcaat 89400 atttgtaatt tgtacttcat ggtgagtcac ttccagagaa tagattatct accatggaag 89460 ggaaaaggtc tgggagtgaa agtgagctgg acaagacaca ggtggaggca ttaggtttca 89520 acagcttcct gcctctacaa agcaaagggc aggaatgtgt gctggcgtgg gctggagccc 89580 agaacagcca caactctcaa gtggaggata agcagaaagc agggttagtc tcctgtttct 89640 ccaggtggtt ctagctgatt cttctaggtc tggaaacaaa tgttaccttc tatccatttt 89700 ctctccttct cccctggctt ccatttttat agctcagaag caaatccatc ttttaagagt 89760 tgttctcaag caatctttgt ctaaagctgg atcctcacca tactacatcc cacttcaccc 89820 ccacctccac cctcactgcc aattgaggaa gttctctgaa ttctgacacc tccatagcat 89880 gtgatagtcc cctatgttat tcctatgcat caattcattt ttacattgtg ctggagaact 89940 ccatctctct tacaagagta gatgatcatt aaggtttggt ctatagaact ccaaatagac 90000 tggactagaa aagaaattcc ttctgacaca taataatcag aacaacaaat gcactaaata 90060 aagacagaat attgaaagca gtaagggaaa aaggtcaagt aacatataaa ggcaggccta 90120 ttagaattac accagacttc tcaccagaga ctatgaaagc cagaagatcc tggacagatg 90180 ttatgcagac cctaagagaa cccaggccag gctactatac ccagccaaac tctcaattac 90240 catagatgga gaaaccaaag tattccatga taaagccaaa ttcacacaat atctttccac 90300 aaatcgagcc cttcaaagga taataaaggg aaaacaccaa cacaaggaca gaaactacac 90360 cctagaaaaa gcaagaaaat aatctttcaa caaacctaaa agaagactgc agcaagaaca 90420 gaatccaaac tttaacaaca aaattaacag gaagcaacaa ttacttttct ttgatttctc 90480 ttaacaccag tggactcaat tgcccaatga aaagacatag actaatagac tggctacaca 90540 aacaggaccc aacattttgc tgcttacagg aaacccacct cagggaaaaa gacagacact 90600 acctcagagt gaaaggctgg aaaacaattt tccaagcaaa tggtctgaag aaacaagctg 90660 gagtagccat tctaatatcg aataaaatcg acttccaacc caaagttatc aaaaaagaca 90720 aggaggggca cttcatactc atcaaatata aaatctacta aggtaaattc tcaattctta 90780 atatctatgc tccaaatata agggcagcca cattcattaa agaaacttta gtaaagctca 90840 aagcacacat tgcacctcac acaataatag tgggagactt caacacctca ctctcaccaa 90900 tggacagatc ctggaaacag aaactaaaca gagacacatg gacactaaca gaagttataa 90960 aacaaatgga tctaatagat atctacagaa catcttatcc taaaaaaaag gatatacctt 91020 cttctcagca cctcatggta ccttctccaa aattgaccat ataattggtc acaaaacagg 91080 cctccataga tacaaaaata ttgaaattat ctcatgcatc ctacctgatc accacggact 91140 aaggctgatc ttcaataaca gcataaataa tagaaagcca acattcacgt ggaaactgaa 91200 caagactcta ctcaatgatt ccttggtcat agatgaaata aagaaagaaa ttaaaaactt 91260 tttagagttt aatgaaaatg aagccacaac atacccaaac ttacgggaca caatgaaggc 91320 agtcctaaga ggaaaactca tagccccaag tgcctccaaa aagaaactag agagagcata 91380 cactagcagc ctgcagcaca cctagaagct ctagaactaa aggaagcaaa ttcacccaag 91440 aggagtagat ggcaggaaat aatcaaactc agggctgaaa tcaaccaagt ggaaacaaaa 91500 agaactattc aaagaatcaa ccaaaccaga agctggttct ttaagaaaat caacaagata 91560 gataaaccct tagccagtct aactagaggg cacttgtatc cagtctaact agagggacag 91620 tatcctactt aacaaaatca aaaatgaaaa aggagacaac aacagatcct gaggaaatcc 91680 aaaacatcat cagatcctac cacaaaagtc tatactcaac aaaactagaa aacctggatg 91740 aaatggacat cttcctaggc agataccagg taccaaagtt aaatcaggat tagattaacg 91800 atctaaacag tcccatttct cctaaagaaa tagaagcagt cattaatagg ctcccaacca 91860 aaaaaaaaaa aaaaaaaaac caggaccaga tgggtttagt gcagagttct atcagacctt 91920 caaagaagat ctaattccaa ctctcctcaa actattccac aaaatagaaa cagaaggtac 91980 tctaccaaat tcattctatg aagccacaat tactcttata cctaaaccac acaaagatcc 92040 aacaaagaaa gagagcttca gaccaacttc ccttatgaat atccatgcaa aaatactcaa 92100 taaaatcctt gcaaaccgaa tctaagaaca tatcacaatg atcatccatc atgatcaagt 92160 aggcttcatc ccagggatgc agggatggct taatatacgg aaatccatca acataatcca 92220 ctatataagc aaactcaaag acaaaaacca catgatcatc ttgttagatg ctgagaaagc 92280 atttggcaaa atccaacacc ccttcatgat aaaagtctta gaaagatcag gaatttgagg 92340 cccataccta aacataataa aagcaatata cagcaaacca gtagccaacc tcaaactaaa 92400 tggggagaaa ctcaaagcaa tcccactaaa atcagggact agacaaggct gcccactttc 92460 tccgtactta ttcaatatag tacttgaagt cctagccaga gcaattagac aacaaaagga 92520 gatcaagggg atacaatttg gaaaggaaga agtcaaaata atcactattt gcagatgata 92580 tgatagtgta tatataagtg atcttataaa ttccaccaga gaactcctaa acctgataaa 92640 caccttcagt gcagtagctg gatataaaat taactcaaac aaatcaatgg cctttctcta 92700 cacaaaggat aaaagggctg agaaagaaat tagggcaaca acacccatca caataggcac 92760 aaataatata aaataccttg gtgtgactct aactaaggaa gtgaaatatc tgtatgataa 92820 gaacttcaag tctctgaaga aataaattga agaagatctg agaggatgga aagatcttcc 92880 atgcaggatt aattggcagg attaatatag tcaaaatggc tattctgcca aaagcaatct 92940 atagattcaa tgcaatcccc ataaaaattc caacataatt cttcacttag ttagaaaggg 93000 caatttgcaa attcatctgg aataataaaa aacataggat agtaaaatct attctcaaca 93060 ataaaacaac ctctggtgga atcaccatac ctgaccttaa gctgtactac agagcaattg 93120 tgatgaaaac tgcctggtac tggtacaatg acagataggt agatcaatgg aatagaattg 93180 aaggcccagc aatgaatcca cacagctgtg gtcactggat ctttgacaaa ggagctaaaa 93240 ccatccagtg gaaaaaaggc agcattttca acaaatggtg ctggcacaat tggcggttac 93300 catgtagaag aatgggaatt gatccattct tttttttttt ttttttccat tttttattag 93360 gtatttaact catttacatt tccaatgcta taccaaaagt cccccatatc cacccacccc 93420 cactcccctg cccacccact cccccttttt ggccctggta ttcccctgta ctggggcata 93480 taaagtttgc aagtccaatg ggcctctctt tccagtgatg gccgactagg ccatcttttg 93540 atatatatgc agctagagtc aagagctccg gggtactggt tagctcataa tgttgttcca 93600 cctatagggt tgcagatccc tttagctcct tggctacttt ctctagctcc tccattggga 93660 gccctatgat ccatccatta gctgactgtg agcatccact tctgtgtttg ctgggccccg 93720 gcatagtctc acaagagaca gctacatctg cgtcctttca ataaaatctt gctagtgtat 93780 gcaatggtgt cggcgtttgg atgctgatta tggggtggat ccctggctat ggcagtctct 93840 acatggtcca tcctttcatc tcagctccaa actccgtctc tgtaactcct tccatgggtg 93900 ttttgttccc aaatctaagg aggggcatag tgtccacact tcagtcttca ttctccttga 93960 gtttcatgtg tttagcaaat tatatcttat atcttgggta tcctaggttt ggggctaata 94020 tccacttatc agtgaataca tattgtgtga gtttctttgt gaatgtgtta cctcactcag 94080 gatgatgccc tctaggtcca tccatttggc taggaatttc ataaattcat tctttttaat 94140 agctgagtag tactccattg tgtagatgta ccacattttc tgtatccatt cctctgttga 94200 ggggcatcta ggttctttcc agcttctggc tattataaat aaggctgcta tgaacatagt 94260 ggagcatgtg tccttcttac ctgttggggc atcttctgga tatatgccca ggagaggtat 94320 tgctggatcc tccggtagta ctatgtccag ttttctgagg aaccgccaga ctgatttcca 94380 gagtggttgt acaagcctgc actcccacca acaatggagg agtgttcctc tttctccaca 94440 tcctcgccag catctgctgt cacctgaatt tttgatctta gccattctga ctggtgtgag 94500 gtggaatctc agggttgttt tgatttgcat ttccctgatg attaaggatg ttgaacattt 94560 tttcaagtgc ttctctgcca ttcggtattc ctcaggtgag aattctttgt tcagttctga 94620 gccccatttt ttaatggggt tatttgattt tctgaagtcc accttcttga gttctttata 94680 tatgttggat attagtcccc tatctgattt aggataggta aagatccttt cccaatctgt 94740 tggtggtctt tttgtcttat tgacggtgtc ttttgccttg cagaaacttt ggagtttcat 94800 taggtcccat ttgtcgattt tcgatcttac agcacaagcc attgctgttc tgttcaggaa 94860 tttttcccct gtgcccatat cttcaaggct tttccccact ttctcctcta taagtttcag 94920 tgtctctggt tttatgtgaa gttccttgat ccacttagat ttgaccttag tacaaggaga 94980 taagtatgga tcgattcgca ttcttctaca cgataacaac cagttgtgcc agcaccaatt 95040 gttgaaaatg ctgtctttct tccactggat ggttttagct cccttgtcga agatcaagtg 95100 accataggtg tgtgggttca tttctggatc ttcaattcta ttccattggt ctacttgtct 95160 gtctctatac cagtaccatg cagtttttat cacagttgct ctgtagtaaa gctttaggtc 95220 tggcatggtg attccgccag aagttctttt atccttgaga agactttttg ctatcctagg 95280 ttttttgtta ttccagacaa atttgcaaat tgctccttcc aattcgttga agaattgagt 95340 tggaattttg atggggattg cattgaatct gtagattgct tttggcaaga tagccatttt 95400 tacaatgttg atcctgccaa tccatgagca tgggagatct ttccatcttc tgagatcttc 95460 tttaatttct ttcttcagag atttgaagtt tttatcatac agatctttca cctccttagt 95520 tagagtcacg ccaagatatt ttatattatt tgtgactatt gagaagggtg ttgtttccct 95580 aatttctttc tcagcctgtt tattctttgt atagagaaag gccattgact tgtttgagtt 95640 tattttatat ccagctactt caccgaagct gtttatcagg tttaggagtt ctctggtaga 95700 atttttaggg tcacttatat atactatcat atcatctgca aaaagtgata ttttgacttc 95760 ctcttttcca atttgtatcc ccttgatctc cttttgttgt cgaattgctc tggctaatac 95820 ttcaagtact atgttgaaaa ggtagggaga gagtgggcag ccttgtctag tccctgattt 95880 tagtgggatt gcttccagct tctctccatt tactttgatg ttggctactg gtttgctgta 95940 gattgctttt atcatgttta ggtatgggcc ttgaattcct gatctttcca aaacttttat 96000 catgaatggg tgttggatct tgtcaaatgc tttttctgca tctaacgaga tgatcatgtg 96060 gtttttgtct ttgagtttgt ttatataatg gattacattg atggattttc gtatattaaa 96120 ccatccctgc atccctggaa taaaacctac ttggtcagga tggatgattg ctttaatgtg 96180 ttcttggatt cggttagcga gaattttatt gaggattttt gcatcgatat tcataagaga 96240 aattggtctg aagttctcta tctttgttgg atctttctgt ggtttaggta tcagagtaat 96300 agtggcttca taaaatgagt tgggtagagt accttctact tctattttgt gaaatagttt 96360 gtgcagaatt ggaattagat cttctttgaa ggtctgatag aactctgcac taaacccatc 96420 tggtcctggg ctttttttgg ttgggagact attaataact gcttctattt ctttaggtga 96480 tatgggactg tttagatggt caacttgatc ctgattcaac tttggtacct ggtatctgtc 96540 cagaaatttg tccatttcgt ccaggttttc cagttttgtt gagtatagcc ttttgtagaa 96600 ggatctgatg gtgttttgga tttcttcagg atctgttgtt atgtctccct tttcatttct 96660 gattttgtta attaggattt tgtccctgtg ccctttagtg agtctagcta agggtttatc 96720 tatcttgttg attttctcaa agaaccaact cctcgtttgg ttaattcttt gaatacttct 96780 tcttgtttcc acttggttga tttcacccct gagtttgatt atttcctgcc gtctactcct 96840 cttgggtaaa tttgcttcct ttttttctag ggcttttaga tgtgttgtca aggtgctagt 96900 atgtgctgtc tctcgtttct tcttggaggc actcagcgct atgagtttcc ctcttagaaa 96960 tgctttcatt gtgtcccata ggtttgggta cgttgtggct tcattttcat taaactctaa 97020 aaagtcttta atttctttct ttattccttc cttgaccaag gtatcattga gaagagtgtt 97080 attcagtttc cacgtgaatg ttggctttcc attatttatg ttgttattga agatcagtct 97140 taggccatgg tggtctgata ggatacatgg gacaatttca atatttttgt atctattgag 97200 gcctgttttg tgaccaatta tatggtcaat tttggagaag gtcccgtgag gtgctgagaa 97260 gaaggtatat ccttttgttt taggataaaa tgttctgtag atatctgtca ggtccatttg 97320 tttcataact tctgttagtt tcactgtgtc cctgtttagt ttctgtttcc acgatctgtc 97380 ctttgaagaa agtggtgtgt tgaagtctcc cactattatt gtgtgaggtg caatgtatgc 97440 tttgagcttt actaaagtgt ctctaatgaa tgtggctgcc cttgcatttg gtgcgtagat 97500 attcagaatt gagagttcct cttggaggat tttacctttg atgagtatga agtgtccctc 97560 cttgtctttt ttgataactt tgggttggaa gtcgatttta tccgatatta aaatggctac 97620 tccagcttgt ttcttcagtc gatttgcttg gaaaattgtt ttccagcctt tcactctgag 97680 gtagtgtctg tctttttccc tgagatgggt ttcctgtaag cagcagaatg ttgggtcctg 97740 tttgtgtagc cagtctgtta gtctatgtct ttttattggg gaattgagtc cattgatatt 97800 aagagatatt aaggaaaagt aattgttgct cccttttatt tttgttgtta gagttggcat 97860 tctgttcttg tggctgtctt ctttttggtt tgttgaatga ttactttctt ggttgttcta 97920 gggcgtgatt tccgtccttg tattgcttct tttctgttat tatcctttga agggctggat 97980 tcgtggaaag atattgtgtg aatttggttt tgtcgtggaa tactttggtt tctccatcta 98040 tggtaattga gagtttggcc gggtatagta gcctgggctg gcatttgtgt tctcttagtg 98100 tctgtataac atctgtccag gctcttctgg ctttcatagt ctctggtgaa aagtctggtg 98160 taattctgat aggccttcct ttatatgtta cttgaccttt ctcccttact gcttttaata 98220 ttctatcttt atttagtgca tttgttgttc tgattattat gtgtcgggag gaatttcttt 98280 tctggtccag tctatttgga gttctgtatg cttcttgtat gatcatgggc atctcttttt 98340 ttatgtttgg gaagttttct tctattattt tgttgaagat attagctggc cctttaagtt 98400 gaaaatcttc attctcatca attcctatta tccgtaggtt tggtcttctc attgtgtcct 98460 ggattacctg gatgttttga gttaggatcc ttttgcattt tgtattttct ttgactgttg 98520 tgtcgatgtt ctctatggaa tcttctgcac ctgagattct ctcttccatt tcttgtattc 98580 tgttgctgat gctcgcatct atggttccag atctctttcc tagggtttct atctccagcg 98640 ttgcctcgct ttgggttttc tttattgtgt ctacttcccc ttttagttct agtatggttt 98700 tgttcatttc catcacctgt ttggctgtgt tttcctgctt ttctttaaga gcctgtaact 98760 ctttagcagt gctctcctgt aaatctttaa gtgacttatg aaagtccttc ttgatgtcct 98820 ctatcatcat catgagaaat gtttttaaat ctgggtctag attttcggtt gtgttggggt 98880 gcccaggact aggtggggtg ggagtgctgc gttctgatga tggtgagtgg tcttgatttc 98940 tgttagtagg attcttacgt ttgcctttcg ccatctggta atctctgaag ctagctgttt 99000 tagttgtcac tgttaagagc ttgttcttca ggtgactctg ttagcctcta tgagcagacc 99060 tggagggtag cactctcctt agtttcagtg ggcagagtat tctctgcagg caagctctct 99120 tcttgcaagg caggtaccca gatatctggt gttcgaacca gactcctggc agaagttgtg 99180 ttccactcac tagaggtctt aggatcacgt gtggaatcct gtgtgggccc ttgcgggtgt 99240 caggcgactc agctggcaag gtagccgggg ctcgagtgga gtggaagggg tttgtgcccc 99300 agatcaagcc cgggtagcct gcttccctat gtaccgcagt ctcaagttcc acgcgattgg 99360 attggggtag gcgctgtgtt ccactcacca gaggtcttag ggtcccgtgg ggagtcccgt 99420 gtggaccctt gcgggtgttg ggcaagactc tgctggcaag gtagcccggg gctcgagtct 99480 cgagtcgagc agaagggact tgtgccccag atcaggcccg ggtagcctgc ttccctatgt 99540 accgcagtct caagttccgc gcgattggat tggggcaggc actgtgatcc actcaccaga 99600 ggtcttaggg tcccgtgggg agtcccgtgt ggacccttgc gggtgttggg caagactctg 99660 ctggcaaggt agcccggggc tcgagtctcg agtcgagcgg aagggacttg tgccccagat 99720 caggcccggg tagcctgctt ccctatgtac cgcagtctca agttccgcgc gattggattg 99780 gggcaggcac tgtggtccac tcaccagagg tcttagggtc ccgtggggag tcccgtgtgg 99840 acccttgcgg gtgttgggca agactctgct ggcaaggtag cccggggctc gagtctcgag 99900 tcgagcggcg aattgatcca ttcttatctc cttgtacaaa gctcaaaagt aagtggatta 99960 aagacctcca tataaaagca gagacactga aatttataga ggagaaagtg gggaaaagcc 100020 tcgaagatat gggcacaggg gaaaaattcc taaacagaac agcaatggct tgtgctgtaa 100080 gatcaagaat tggaaaatgg aacctcacaa aattgcatag cttctgtaag gcaaaagaca 100140 ccgtcaataa gacaaaaagg ccaccaactg attgggaaag gatttttacc aatcctaaat 100200 ctgatatggg actaatatcc aatatataca aagagctcaa gaagttagac tcccgaaatt 100260 caaataatcc cattaaaaac ggggtacaga gctaaacaaa gaattctcaa gtgaggaata 100320 ccaaagggct gagaagcacc tgaaaaaatg ttcaacatcc ttaatcatca gggaaatgca 100380 aatcaaaaca accttgagat tccacctcac accagtcaga atggctaaga tcaaaaattc 100440 aggtgacagt agatgctggc gaggacgtgg agaaagagga ataatcctcc attgctggta 100500 ggattgcaag cttgtacaac cactctggaa gtcagtcttg tggtttctca gaaaattgga 100560 catagtactg ctggaagatc cagcaatacc tctcctgggt atatacccag aagatgttcc 100620 aactggtaat aaggacacat gctccactat tttcatagca gtgttattta taatagccag 100680 aagctggaaa gaacccagat gtccctcaac agaggaatgg ataaagaaaa tgtggtacat 100740 ttattcaatg gagtactact cagctattta aaacaatgaa tttatgaaat tcttgggcaa 100800 atggatggat ctggaggata tcatccttag tgaggtaacc caatcacaaa agatttcacc 100860 agatatgcac tcagataagt ggatattaac ctagaaactt agaataccca agatacaact 100920 tccaaaacac aagaaaatca agaagaagga agaccaatat gtggatactt agaataagga 100980 acaaaatagc catgaaagga gttatagaga tgaagtttag agttaagaca aaagggtgga 101040 ctatccagag actaccccat cttgggatcc atcagccact aaacccagat actattgcat 101100 atgccagcaa gattttgctg aagggaccct gatatagctg tctcatatga ggctctgcca 101160 gtgcctggca aatacagaag tggatactca cagtcatcta ttggatggaa cacagggcct 101220 ccaatggagg agctagagaa agtacccaag gagctgaagg gatctgcaac cctatagatg 101280 gaacaagaat atgaactaac cagtaccccc agaggtcata tctctagctg catatgtagc 101340 agaagatggc ctagtcggcc atcagtggga agagaggccc ctaggtcttg caaactttat 101400 atgccccagt acaggggaac gccagagtca agaagtggga gtgggtgggt aggggatcag 101460 ggtcgggaga gggtataggg aactttcggg atagcatttg aaatgtaaat aaagaaaaca 101520 tctaataaaa tagcaataaa gaataaccac tacaaagaga gaaaaggaca acaaaagcct 101580 gcagtaagtt tgctagctga acagagcaga tgaataaccg gcagcagact ttgcaccaca 101640 tcatttcctt tggccatccg cacagattgc cggaacaggc aaaccagatc tccatgcatt 101700 ctgttgtccc caccctctgg gccataggac ttggacaaag gtaatgtcct ggatctgaaa 101760 gaagttaaga cactgggatg gtatttaatt aaataataac agaggctcaa ctcggtgatt 101820 ggcctggctg gaccagcctg atgtgttgat aattgtctaa tcctctagtg gatatcaaaa 101880 aaaatcattg ctttcccaag ggaaggggac agccaggaga aggagaggta tacatggatg 101940 ataaacgttc cagatactgg ctaagtgctt accagtaaat tgaagtccac atttcacagc 102000 atactccagg aagtcatttc tccccagcca gactcaacag tctttgaagg cttctaaagt 102060 ccagctggga ggtagtgttg agcctctagc atgtctattc atagactact agatcccatg 102120 agagctgcca ttacaattgc tggagtacat tttttcactt cctatcacct aaataaactt 102180 cacccactgt gtgggtttgc taatcccttc ctcatccttg ctgtatccac caccctgtct 102240 tgtttcctta gcaactagac actgctgagg gcagggcaga aggctaaaag ggagcaccag 102300 taagtggaat gagtgagtgg tggcttggtg ggctgcagag gggttagttc tcacgttgcc 102360 tttttgagta ttccccatca ttttctcttc tagtgcgcaa gtgcagggtc ctcacatcta 102420 tccacactgt ccacacagag agaacacatt tttaaaagct agcccaaaga gagaacaaag 102480 agctgaggat aactattcct aacccaggat gtctgcctta cattactttt taggtacttt 102540 gcatttgttt gctgctacta tcattgtttg tttactatat tttaggagtt cgaacaatcc 102600 caggcaccat tcagacagaa agtggcaaat gtcatctaaa agggcagggc ttaacattcc 102660 cgtggcagtg tgtgcttccc cagtcagaga gagcactttc ttgcttttac tgttttctaa 102720 ctcgagattt cccccgcttt tctggagctt tttttttttt gttgtttagc ttctcatcaa 102780 gaaagtataa gttgtaggct gcgggagtgt ctgtggggat gcgtttgttt tgtttttgag 102840 acagagtttg gctatttaga gaaggctagc tttgaactcc cagagaccca cctgcttgtc 102900 tcaagtgctg gcactgaaag tatgtgtcca catacctggc ctcgtgtact aattttttga 102960 taattacaaa aaagggcaag aaaaaaagaa agaaagaaaa tttctttctt cagccattca 103020 ttctgtagcc tgttgtgtcc aacactgatc taggcattct tttggggtac ttgagcagaa 103080 agactagact gtgtactttc tagctgggct cttcaccagc aggtgcacat ttcagtattt 103140 atttggccac ttgaaaatct cactgttgat tttgtgataa aaaaaaaaaa caaaacccag 103200 gaacttattt gtaaaacgat tattcctggt gtttatctga ctcaccttat catctgtgtt 103260 taaagattgg tggggtacac tctgagctat ttgtacttgg caagatcccc aactctgaga 103320 gcaaggtttt agctggctag ttttcctcct tcagcctctg ccaagtgctt ggtcctctag 103380 agaataatat gtaccttttg tccccacata ttaaatacaa ataaagagaa gaacatgtca 103440 tcttgaggca ttggaagcta ttaaggctat ctcaggcaat ctggacaact ctacgccttg 103500 gtcacatagt ggcttcttaa tatgtaccat ggctcactca agaaaaatgg agtggtgacc 103560 aacttcagcc tctgagtttt gagatggaga ctctgagata gctgtcaata ttttgtaaaa 103620 ctgcttccta acatcccgta cccctttctg tatgtatatc tgcatgcaca tgcacacata 103680 tgggtataga gcccgcactg gccaaaagat gagagcagat ctccctggat ccagaattat 103740 atatggttgt gagcttccct atattgctgc tgagagccaa tccagttctc tggaagagca 103800 atactcactc tcaactgctc agtcgcctct acaaaccaac tgttttttgt ttgtttgttt 103860 gtttgtttgt ttgtctgttt ggtttggttt ggtttctctt agacagggtc tctgactggg 103920 accgaggctt gctgagtagg gtaggctatt tgaccagcag ccctcaggaa tgcaagtatt 103980 ttcctcaaca tctgatttcc tctttccttt ctccctccct ccctccctcc ctccctccct 104040 ccctccctcc ctcccttcct taatttcttc ctttcttctt tctttcttcc tccttccccc 104100 tctcttcccc tctctctctt tctttttttc cattgccgac atgaagaagt agagatgagc 104160 atgaccaagg tcagtttctg cttttcctgt gacaagggtt ctccccagtc aaccctggtc 104220 tgcccacaga attcttatcc cactgaaagg aatagacatt tccctacatg aagagttgta 104280 aagtggtggt tggtcccatt gcatgcacag agagtgagag acgggtaacc tgtgagacac 104340 ctcctacata aaaagagttc cagtgatctt caaagtgttt gtcactcacc cagaaaaact 104400 ccagctcctc actaccaagc ctaggaaagg aagacagaaa tacctagaga ggaattccaa 104460 tcacctcaag aaaaatggag tagtgaccag cttcagcctc tgagttttga gatggagact 104520 ctgagagagc tgtcaatatt ttgtaaaacc gtttcccact cgatagcatt cccagagtga 104580 aggtttttct ctcctcagcc atgagctagg cttctggatt tctacatcct gtttcatttg 104640 tgttcttcct cctatattta agtgtaagct tgaatgagcg tctcagttca cttcccacaa 104700 tacgagaatt tcccaaacaa ggagggcaca ccaaactcat gttagcagtg cctcttcagg 104760 tgttgcccat ttttctcctt caaaagtccc caagcctccc aggtactact ttgccagttc 104820 aggttgttca ggggaggtac accaagtgct gattcccact gagctcctac tgaccctatt 104880 cagtattgtg ccacagttta cccttctctt ctcttctctt ctcttctctt ctcttctctt 104940 ctcttctctt ctcttctctt ctcttctctt ctcttctctt ctcttctctc ctctcctctc 105000 ctctcctctc ctctcctctc ctctcctctc ctctcctctc ctctcctctc ctctcctctc 105060 ctctcctctc ctttcccctc ccctcccctc cccctcccct cccctcccct ccccctcccc 105120 tcccctcccc tcccctcccc tcccctcccc tcccctcccc tcccctcccc tccttttctt 105180 ttcttttctt ttcttttctt ttcttttctt ttcttttctt ttcttttctt ttcttttctt 105240 ttcttttctt tgtgataggc ttgggaagct tcccctgcag gactgtggag ctcttgtgtt 105300 cacatttgtg ccttttggaa agatgcttca gcggctggac ccgaggcaag agtttgtctt 105360 caaaatgctg ccattgcctc tgtcagtttt catctgcctg taaacttttt ttttttcttt 105420 ttgccaaatc tcctgaagca acttgacctc aaggtttttt ttttttaatt ccctcaactc 105480 tttctttatt ctttgttcca ttttcactat cttttttttc ttattagtta agagaaacca 105540 aagaattgat tcacctgctc attagttttc tcgaatattc ccctgtgact aaagtaaaac 105600 taggctacac ccctaagaga aagatgcctc ttagggctcc tgtcatcttc ctattgtctt 105660 gactggtgtg catacaaagt ttgaactatg aaactagggc atggctgatt ttggctccag 105720 tgtttacttg tgaaccagtc tattcactat tgacctagct ttcagattcc acagcccttc 105780 tagcttccct aacataaaaa cataagcatt atatgtattt aggaagtatc atgttgtgtt 105840 ccaataatat ttatgcagtt tgacctcagt tgtgccctta atccttgtag tacctgtttt 105900 agttagggtt ttactgctgt gaacaggcac catgaccaag tcaactcata aaaacaacat 105960 ttagtttgag ctggcttata aattcagagg ttcccaccaa tgtcatcaag gtgggaacac 106020 gatagtgttc aggccgatat ggtgcaagag gagctgagag ttgtacatta tcataggaag 106080 gctgctagca gaatagtgac ttccgggcag ctaggatgaa gatcttaaag cccacaccca 106140 caagagcaca cctactccaa caggaccaca ccttctaata gtgccactcc ctgggccaag 106200 catattcaaa gcatcacagt atcttaatta gaaaagtggg agctatccct tgttcatctg 106260 tcttagttgt cttgactgct ataacaaaac acctcaaact cattcattca caaag 106315 <210> 5 <211> 171477 <212> DNA <213> Artificial <220> <223> Synthetic <400> 5 ggtgttcctt ggcaactcac tttttctttt taaaaattgt gtgtgtgtgc tgtgtgtgta 60 tgtgtgagtg ggtaagtagg tgtacagcag ccaggggagg atctcagatg tcttccccta 120 tcattctgcc ttgtgccttg tgcctttgag gcagaacatc ttggtgaacc cagagcttat 180 atatgtagac acacacacag atatataaac acattatatg tatataaata tatataatgt 240 gataccataa tatagatata tacacaagac acacacacat atatatgtaa tataatatat 300 gtaatttata tacacatatg tatatgaata tatatatata atatgctacc ataatataga 360 catatactag acacacatat atgtaatata tacaaatatg tatataaata tataatttat 420 gctatcataa tatatatacc ctagacatat atgtataata taacacatat atgtgatata 480 tgtgtgtgtg tgtatataca tatatatata tatatcacgc acacacacat acatacgtct 540 agggtagcaa ccaatcctag taatcctcct gttttcattc ccctcaatgg tctacaggca 600 tgtatgagac cacatatggc ttttatgggt gtatgtgtga tctaaactca gaccttcacg 660 tttgcataat aatcactgtt aactgctgag ccatctctcc ttctctcact ttcctttctt 720 tttgaaaaaa aattttttat acattatgtt tttattttat tttacttatt tttatttttt 780 taatattaga tttttttctt tatttacatt tcaaatgtta tcccctttcc tggtttcccc 840 tccaaaaacc cctatcccat ccccactccc cctgttcacc aacccaccca ctcgcttccc 900 tgtcctggca tgcccctaca ctggggcata tacattaggt tttgagcgaa ttccaggata 960 gccagggcta cacagagaaa ccttgtcttg aattaaaaaa aatttttttt ttaaattaga 1020 gttgctgcca tgagtatgag caacttaaga aaattatctc tcgccgggca gtggtggcac 1080 acgcctttaa tcccaacact tgggaggcag aggcaggtgg atttctgagt tcaaggtcag 1140 cctggtctac agagtgagtt ccaggacagc cagagctaca cagagaaacc ctataaaaaa 1200 ataacacaca cacaaaaaaa atcaaaaaca aaaacaaaca agagagagag agagagagag 1260 agagattctt tccctccaac ttctcccaga ccttccctac ctccattccc accaacttca 1320 tgtttttttt tctctctctc tctttctcaa aaccaaaaaa tgccaggaaa gcaacaaaaa 1380 ataagtagca agaaagtcag aacaaagaaa ttaaatacgt aagtaagtaa ataaaaatag 1440 aaaatcagca atattaaaaa aaaatcaagg actggagaga tggctcagca gttaagagca 1500 ctgattgctc ttccagaggt cctgagttca aatctcagca accacatagt ggctcacaaa 1560 catctgtaat gggatccgat gccctcttct ggtgtgtctg aagagagcta cagtgtactt 1620 acgtttaata aataaatctt taaaacaaga caacacaaaa atccaaaata aaatttcaaa 1680 gtgtaccaac acaaaacatg tagtctgttt tgtgttggtc aactactcct gggtatttag 1740 gcttccttgg agtgtggttg ctatacccag tgacactcag ttaagaaaag tgaatttccc 1800 tttcctggca gggatcaatt gcaagtagct ttttggttag gggtagaata ttatattcca 1860 ccttcctgtg ctggttccac ctgtaaatgt ctgtgcagag ctgtttatgt ttacgtctca 1920 ggcagagctc tgaggaagat attagtaagc aagacattcc ttggagagtg cttgggggat 1980 ccagcaactg tgaaaggaaa aagaaatatt aatcaaaggg ataaattaac cttcaataca 2040 gtttcagcaa aggcctctca tggtgctgtc cccagttgag gcaaggcaac agaccttttt 2100 cctcaccaaa cactcactgg tgtaggggca tggtatgacc tgtcatcagg cagggaacat 2160 tcaccacaca gagtggagag gagctgtggc catctcttca atcagagcag ggtgttggat 2220 ggacgttgcc agcctgtacc acccaccatt cacatcagct gctctgtgca cacagcacac 2280 tccaacccac agagaaggtt tgtttctgtt tttatttttg ctttttatta ttatctcatg 2340 taaaattatt atttatttta tttttgcttt ttttattatt atctcatgta acccaggttg 2400 gctttaaatt tgttatgtag tcaaggagga ctttgaactt cttccctcac ctctagagtg 2460 ctggggtcac aggcacgtat cactattcct gttcacctgg tacttggaat caaacccagg 2520 cttcatgaat aatagaccta aactcttcca aaccaagaag aaccacatct cacgagattt 2580 gacatgctgt gggttttttt ttttcctctc ccccctctgt ctctctcctc ttctgtccct 2640 ctttctccct cttctttatt tctttcattt ttatttttac ttttgaaaag aagctggctt 2700 caaaattctc catatggctg aggacgactt cagcctttaa tttctttttt tgtttgtttg 2760 tttgtttttc taggcagggt ctcactacac agccctggct gtccttgaac tcactatgca 2820 gactaggctg gcctcgaacc tggagatctg cttgcctctg cctcctcagt gctgaaattg 2880 aagacttgca ccaccaaggt atttttagtt aatcgtttga aaaatttata catgtttaca 2940 atgtattttg atcaaatcca acagaaatct tcctctcact tcttctcagt ccccctacat 3000 ctttcctacc ataatttctc tctttctctt ctttttattt ttgaaataac ccaccaagtc 3060 cagatagtac tgtctacaag tgcataaatg tagagccatc ggctaaagca tggccaggct 3120 actagtaatc ccactgaaca ttacacacgt tctttggtca aattaagcaa gcttaatttt 3180 ctgttagaca gggatagctc cctaaatcag actttgataa tatagcatga cacttaagag 3240 aaagtggggc ttgtagagcc tccaaaacct gcaagcctgg gggtttttta aggcttggga 3300 gacttccaga ggaattttga ttgcatagga acttggctag cagaacatct tatcttatca 3360 gggtgcattc aaggtgtgag tcaggctcca gagggtgggg agcaagtcgg attccagaaa 3420 cacacagtga gtgtttggtg aggaaaaagc acagtcaacc tgaattttgc agcagacaga 3480 aatgaacttc tgaccttata acaaaatggc ttccgtcctt aagatggcgt caggctggtc 3540 catcagtaac tacacccctg aagaaaagtt atttcctctc cctaactgtc accaattgcc 3600 agcaacttct cagctaggaa tagagcctca tgatttcctc tcccactcat actgggtgtt 3660 gactggcttg agtgccatgc cacgtcctga agacagcatt tcacagcatt tgtccctatc 3720 ctctggctcc tacggttctt ttgttccctc tgctgagatg taggtacccc gagccttgta 3780 gtagaagtga caggcatgtc ccatttatgg atgactactc aatggtcaca tactctcaga 3840 actgtggcca gttatgagtc tgtgttgact gctgcctact gcaaaaggag tcttctctgg 3900 gcaaaacagt agtaatctac atgtataaaa cacaaatgct tacaaggtag tttgatgggg 3960 gggggggact gtcaaacaac ccttgcacag gggtcaccta ataccatctg cacaggggat 4020 atttgtgcct tccctcagcc ttgagtaggc taaacaaacc catgatagtt ttacaaccta 4080 gctggagtct tctgacctca ccacatttgc aatttcctat agagctttga ggagtagact 4140 gcccactgag cttgctttgc aacataatct ggctgaaacg aaggatgggg tttgtgggct 4200 ttctcagtgc tgaaaattaa ctttgagcct tgatcagaaa cgtttgtctt tgcttcatcc 4260 ttctcttgca cccagttttt ttctcagttc caggcccccc cccgccccgc ccctttcagg 4320 tatacccagt ttgtccatgg ccactggaca gctacagata tttacattac gattcacaac 4380 agtagcaaaa ttagttatgg agtagcattg aaatagtttt atggttgggc atcaccataa 4440 cacgaggaac tgtcttaaag ggtcacagct ttaggaaggc tgagaaccac tgtcctgcag 4500 cctagctctc atgggtcctt gataaggttt gtagcaccag acatgaactt tttcctgtgg 4560 agcagccctc aaagacagtc agaaagagca gttggttatc ccaacaacca acatgtcact 4620 attatatcaa gggggctttc ctatagcgtg aatggtcctc agagggtaag tccatctttt 4680 ctccccacgt cgcagggata gcatttcctg gcaccatgaa agcaggccag tagaaaggaa 4740 gtttctaagg accttgtact tctgatcctc ctcctcgcat gtgttgggat tacaggcctg 4800 caccaccaca tccagtttat taggggctag gcatgtgcac agtaagccaa cactcaataa 4860 gcagaacgac actcagacct cagccccttc atttagtgtt agtgtgttgg tggttgttta 4920 ttattattat tattattatt aattcaccac acagcaattt atgtcctaca tgtcacattc 4980 atttttaaat tccctttact ttttccccta ctttgtttta gaatatatgt atatgtgtat 5040 gtgtatacac gcacacacac acatacatat atatgtgtat acacacatac atatatgtgt 5100 attcatgcat atacatatgt gaatatacac acatacacac gtatatatga gtatacacac 5160 atatacatat ataatatgtg cgcacacata tattttatat attagcattc cagcttaggg 5220 gcttcagtgt agctcattgg cacagctctt gtctagcgtt ccagttttat ttctactttt 5280 ggcttttaag tctatttgtt ctattgattt aaagcttaca gtacctcggt gggggcacac 5340 acctttaatc ctagcactct ggaggcaaag gcaggtggat ctccgagttt gaggccatcc 5400 tggtctacag atcgagttcc aggataaacc gaaagtagag aaaccatgtc tcagaaacca 5460 aaagctaaaa caaacagaaa agattcacaa caaatatttt taaatcatta caatatatta 5520 caatatcatt aatattacac ccctttaagg gtagtataag aatctttatg ttatgtgtct 5580 ataaaatggg ttgaggagct ggtgagatgg ttcagtggat gaaggagctt gttagcaagc 5640 ctgccaactt gagttcaatc cctaggaccc acgtgataaa aggagggagt agattctttg 5700 aagttgtcct ctgacctcca catgtatact atgtcacatg tgttcggcat aaaataaaca 5760 aaaacataga agggagccta ggagacagtg ccgttggtac aaagcctgct gcaagagcat 5820 gaagccctga gtttgagccc tagcactcgg tcttatgcat acagcacatg tctgtaatct 5880 cagcactggg gagacaaaag agtgagtggc cctggagttt gctgggctgc cagtcaagcc 5940 aaatcgacta gctccagata gggagtgggg cagagaaaga ctgagcccat ctcaatggtg 6000 ggcaatgatt gataaagaca tccagtcttg ccttctggct tcccataagt gcatccctca 6060 gtgatatact gttacctgga agagtaagcc aaccaagccc tttcctccct aactgatttt 6120 ggttggaaag gaggccattt ccatgaagct aggaattgac cttggatgga agatgctggc 6180 ggaagtttct ggtttagtcc aggtggcccc cgaagtgtgg gctacaccat ttcccagaaa 6240 cctctagtag gcatgcactc cagctaccca tgctggacac tcgttctttg atctctctcg 6300 tttactttct tattttcctt atattatttg ctcgtttatc cccagacatt cctgggactg 6360 tcttttacac aaattcttca catcccatga tgtctttccg gctctccgtc ttggaagaaa 6420 acacccaagt gactgactgt gaccaaacgg ttacatgcaa atgtatgaaa tctggggacg 6480 aatgcagtaa taaaaggtga cttaaagggg ctggagtgat ggttcagtgg ataagagcac 6540 tgactgcact tccagaggtc ctgagttcaa ttcccagcaa ccacatggtt cacaaccatc 6600 tataatggga cccgatgccc tcttgtggtg tttctgagga tagcaacagt gtactcatat 6660 aaataaatca atcttaaaag aaaataaaaa acatttcatt aaactcgata ataggtataa 6720 atgcgaactt aatggatgtt ctcagggaag ttgactgtaa cttgtaattt gttcagtgaa 6780 tagcaagcat ataacaacca gacggctaaa actaccacac cgctaccaca catgtttctt 6840 tacgtatcac atctgcattt ccccttgcct caagcatagt acttggaact ttgttcctca 6900 tattttatga ctgccttcat ttttttagaa aagagttgtt actattatta tatgtaagta 6960 cactgtagct gtctttagac acaccagaag agggcatcat gtggttgctg ggatttgaac 7020 tcaggatctc cagaagagca gtcagtgctc ttaactgctg agccattttt ctagctcaag 7080 tgcctttgtt ctttttctgg agtttctgga tctagacagt gtgccttaat tttgttcttc 7140 agtgatgttt ccagcctgca accgagcttc ccttcctcgg ctggaaaccc atctttgcct 7200 tctagattcc tgaagaggtt gaatttcatg tgctgactac aggtcccttc ttcaaaaccc 7260 attctgccct cagaatctat gaacccgatt taaaaaaaaa aaggattttt atttttatgg 7320 gtttggctat tttgcctgta ttcatatctg tgcaccacat gcatgaagtg cttgcagagg 7380 ccaaaagagg gcgccagata tcccggaact ggagttacag atggttgtgg gccagtgctc 7440 tggaaaaaca gtcagtgctt ttaaccccta agctatctca ccagccctca tcttctctta 7500 gaccgagtgc tagggctcaa actcagggct tcatgctctt gcagcaggct ttgtaccaac 7560 ggcactgtct cctaggctcc cttctatgtt tttgtttatt ttatgccgaa cacatgtgac 7620 atagtataca tgtggaggtc agaggtcaac ttcaaagaat ctactccctc cttttatcac 7680 gtgggtccta gggattgaga agtcatctgt gatttttgac ccagctgccc tacctcattt 7740 tgcagggaac agggatgggg atgggaatgg caaagggcag gacactgttc caccttccgc 7800 ctcaaggctg ttaacaaggt ggtttaatac tgaattgcca gcccagccca gcccagcctt 7860 tcactatttt cccggtattg tcaaacattt cctccacact acctatagaa tattcttact 7920 tatgtttaat gatctacttt agtgtttatt ttaagtacat actatgttcc gttccttgac 7980 ctcgctgtac tttgtttatt cgtaaatata cttacccacc tgttgtaata aaaatgtcct 8040 tctgtccttc cttcctttct tcctttcttc ctttcttcct tccttccttc cttccttcct 8100 tccttccttc cttccttcct tccttctttt ctcccttcct tctttcctcc cctccttcct 8160 tctttcctcc cttctttctt tcctcccttc cctccctcat ttctttaacg ttcaaccaat 8220 gtttctttct cacgtttcct ttccaaggtc agttccgagg gctggacaac cacactaagc 8280 tcagcggctc cgcacatcct gccctcgtgg agcccatgtt aaagagaggt tggtggagac 8340 acgcaatgga cactggagac aacgacttag cttagtaccc taaaagtgaa tggaggatat 8400 gcaaaaataa gatagagggg agagcaaagg aatttggcgt ccagcgttag tgtgtgtgta 8460 ggagtgcgta gtaaagagca gaagctcgtt acgtgtgcag cggatgtgag agtctcacag 8520 agggaacccc cagcgctaag ttcctgaggc ttggtgggtc aaagaacaaa ttaacctcag 8580 catagttacc cttccataag caacaaattc tccaggtcca ctaagcctcg tgatcagcag 8640 cctgtacagt cacttatgag gaacttttat agggagaaca ggatgtgatg gtgtatttaa 8700 acatatgtat ttaaagatgg ctcagtgaat aagggtgctt gttgcccaga cctgagttca 8760 atccttgggc tccacgtggg ggaaggagag aaccaactcc tgatcctcca catgaatgct 8820 gacatgagca tgcacataag caatacatga gtctaatttt aaaaaaggaa aaatacctct 8880 ggttaacata taataaattg ctattctttt agatggttgg attcctaata cacaccagca 8940 cagagtgaac gcactgggta atgtacaata gcaaggtgac ttacatcaaa ctacattaaa 9000 tgtttaaaac agtgaaaacc tgaaaagcta acatttttgt ccaatgtgaa aaaaaagtct 9060 tatttagtag catgatatac acagaggtaa aaatagatat cagaaagtcc cccacatatg 9120 ctaatcaatt ttgttcttaa tctttacata tccacttatt tttgtgatat ctttatttta 9180 tgcataaggg tgttttgcct gaatgtatgc ctttgtaaat acttgcatgc agtacccaca 9240 aaggccagaa gagggcgtga gatcccctgg aattgaagct actgatggtt gtgaaccacc 9300 acgttggtgt tggaaatctg acccaaatgc tctgcaagag tcgccagtgc tctgaaccat 9360 gtccaagtta tctctccagc attatatatc cacgtttgaa gtagaggtac attgggcttt 9420 tcatttttat agtggttact taaagcatag tctatgctgt ttttaagtga cagaatgttg 9480 ttcattgtat ttaaaaaata tagacaaaaa tttaaacact ttaaaaaata ttatttttct 9540 ttatgaagat acatgctttt aaggggttct gttggagaat ttcagtagcg ccaacttaaa 9600 acaaatactt tattagggct gtagaaatga cttagaggtt aatagtgtgc actgctcttg 9660 cagaggatcc gagtttggtc cctagcaccc atgtcagggg cttacaacac aacctcctgt 9720 aactgatgct gcaggggatc ccaccccctc ttctggcccc tctcatgcac aacacacatg 9780 aggcaggcac agactgatgc acatatatgt gaattaaacc ccaaataaaa tcgttttcaa 9840 aagtccaatg aatgtcttga caatgctttt ataatcaatt cattcatgac aggtggttcc 9900 ctgtgtttgt gatttctctt caagctgcgc atcaaagcaa cccctccttc tccctccttc 9960 cctccctcct cctcctcctc ttcctcgttc tcctccctct ctctgtgcat gtgtgcctgc 10020 ttacctgttt gcgcaccgca ggcgtgcagg tgcccacgga gtgattaatg aattctttgg 10080 aactgggtta caggcagttg tgggcgatgt agtgtaaatg ctgggaacca aactcgggtc 10140 ccttggaaga gcagcagtgc tcttcacaga tgagccatcc ctcaagtccc caagaacttg 10200 tttctcttgc tcagcttgca ctctccaaga cttaggaaag tgcttagtga ataagctggg 10260 gcacaaaaga tgcggttata agaaggtgac gtaggagcct ctggcaagcg atgcagatgc 10320 caattctcac ttgtgttccg gtccctctcg cttttgtcag ttttagaatg ttctaggctg 10380 ggtttggtgc tgtcgtagca cagagaggaa acagcatgaa ggacaaccag gccagagata 10440 gttgatggtc tgagcctttt gtttgctggc ttcactcgct ccgctgttct aaagcttcag 10500 tcaatgggtc tatgttgaac cagtttccgc tgttcactac aaagatgcaa ttggaagccg 10560 agggagggag ggagaagctc tggagagtat ggctcagtgg ttaagagcac tgactgctct 10620 tccaaaggtc ctgagttcaa atcccagcag ccacatggtg gctcacaacc atctgtcatg 10680 ggatctgact ccctctgaag acagctacag tgtacttaga tataataata aataaatctt 10740 agaaaacaag tagctgaggg aggagttttt tacttgtctc atccagggtg cctggaggat 10800 gacggcgagg cgccactttg cttacctcac caagtgagag ggacaaaaca tagttttgat 10860 ctgcagtagc ttcgcggtga gcaccacatc tccacctagt cctccgctat tgtcggctgc 10920 tatgaacaga ccctgctgtc acctggccat ttatctccat ggtcctgttt aagctctagc 10980 ctattgacta aggggatgca agttgaagtg gatgtcccca cgtttgaaca cagcactaca 11040 tgctggtatc tgaaatctga gtcacatcta ggtcagtcac attttctaga tttatgagtc 11100 tagattttat ttttccaggt gtcttgtttt tctgtctcta aatgatgaca tccacccccc 11160 tgccttcccc ccccccccca cacaccatat accctgggta tggttatttg tagcaaaatc 11220 aatactcttg tacttgaata atttttttct ttgcattttg aaccaaggtg tttattcaca 11280 aaaggaagaa ttgtccattt gtctgtctgc tcagtctttg gcttagcata gggcatgtgc 11340 agaggctcaa taaaaatctt ttgcctcaat cagtacatgg cacatgcatt acctcagggt 11400 catatttgta aataggtatg ggtgtttgcc tacaggtgtc tgtgcaccac ttgcatgcag 11460 gtgcctgagg aggccagtag agggcatcag atctctggga actggagtta cagatggctg 11520 tgagtcacca tatgggtatt gagtacaaga gagactctgg aagagcagac agtgctctta 11580 gccactgagt catcactgta gcccgcatgc catattatta aggaggctga acaccaagtg 11640 attcagcctc gctacagaca agtagaaacc tgtctctggc ctccttaacc acaaagttgg 11700 ggggcttccc tcgacacaga agccagagaa ccatttgaca ccgttcctct gtcacaggct 11760 gtatcattgg tccattttcc acatgacact gtgcattcaa gcattcatct ctgcagatcc 11820 ctgggcagta tggcagtgtg tgtgtgtgtg tgtgtttgtg tgtgtgtgtg tgcttgtgtg 11880 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtttgtg tttgtgtgtg tgttctaatt 11940 ggaaaggaaa tcttcacttg cctttaaagt gcagtgctac tatatttctt tgcaaatata 12000 tatacgtata tgcatatgta tatgcagatg tatacacaca cacacacaca cacacacaca 12060 cacacacata tctcaaagtt cctttcaggc tgaagaatcc attattgtcg ggccagtgag 12120 atgacagagc agtttaaggt atctgctgcc aagtctgatg acctgagctc aatccctgaa 12180 atccacaccg tggaagggga aaactgtctc ccacgagttg ttttcttgac cgccacaggt 12240 gtatgcaaat atgcatgtgc acgtgcacac aagcactaaa taaacagaaa gtttaaaaaa 12300 ttaaggtata tattatttac tatgtaagag aacatcaaaa aagtcttgag aagtacacca 12360 tgggagaaaa cacacctagc ccttcaaata atttgccctt ctagaaaaat cctgaaatag 12420 ccctttcccc atacagtagt agaatgataa cattatctca gagatgagca ggcgtttaag 12480 gctcagcgga acgggaagag agttgggttt ttcttcctat ttgttttgaa tagctctgct 12540 cccgggtcct ctgcttgccc agatgatctg tgaaatgggt gagtgagcag cgcacctccc 12600 cagcgcgact gttactgacg tcagcaagcc tcattagctg ctgtttgtct aaccaaaggg 12660 aacagcgggt ggagagaatc ttaatgacct gacttcccac actgcaggtg ccacttgcct 12720 ggtgtgcacg ctgagattgc atcagctgac taggcaaaca aggaacagtg gaaatgggag 12780 gcagagaaaa agcccaagag agactgccca gtgagtggaa ctcgctgctc catcctggga 12840 gagtgtagca gagatgtttg tgcgtggtgg agtgaggtcg actcaggttc acagctgtgc 12900 gctgctacat agtagttgag ggtcgaatca cttaaaggct ttagtcccgt tttcaaaatt 12960 aaaataggga taatgataga accttaggga gaaggaggga cacgtgtgta aatcacagac 13020 ctcattgtac cactggataa ctggcaggag ccactgtttc tgactctaca gtggagagaa 13080 gggaagagca atgattggca ctgtggacgc aggtggcaga gaacagctaa agatgacttt 13140 atatcctaaa ggcaagtggg gtagctgtga gttttaaaga ccgaaccttg cttcacaaaa 13200 ttgctcttgc tttcctacct accaattctg cgttctttct ttttccctgg cagcatctcc 13260 ccggcggact cagccttggc tacagcggag actcctggga aggaaatgtc cttccccagc 13320 cctaggcaca gtggttattg tctgttctca catcctgtga gccctgtagg cagaggaatg 13380 ccccagaacc acagcaactg tgcaaacatt tgaattccca cgggtagttt cccatcctcg 13440 tacccatgag gcttctgctc tctgtagtca gaagaacgta cacagttctc aggagcagga 13500 agtagagggc taggaatcat gggtgaggag cctagcatgt gtgtgaatac atccgtggaa 13560 aacgtttttg agggagatag tacagaatcc tactccaggt gaaataatga gactcttgct 13620 aagaccacgt acggatgacc caatcaatac acaaggaggg agttgaaaag gagaaaaggg 13680 gttggccatt aatttagcca acgtataatt ccacgtatta gcaagtaact ttaattatcc 13740 tatgcgccct cggttttatt ctccacagct gtaaatggta ataatacccc ctttgaacat 13800 ttatcatgat taattaaggc agtgttgttg aaacatattg aaagttacct aggctaactt 13860 tgtccaattt gccagctgct ttggcaaatc ctctgcactg gggctggctc aggctagaac 13920 tatcttccat ctgggtaaat ctcttctcca ttccagagca gatatcatgg cgggtagctt 13980 gacctcacag tcatgagaca actcaaacca tggggattgg caaatgaagc aagtccttgt 14040 tattgaactc tccactcaga aaaaataaaa aataaaaaca tattaatact acggaggatt 14100 ccagtgacac tcccttctgg gaaagacact caagagattt ttatgagcag tcagggcaga 14160 tggaagtcac aaggcaaaca cctcaaagta aaaaaaaaaa aaaaaaagat caacatacca 14220 gagcactcaa caagctgaca gtctggctgc ccatgctaga tgcttagaat acatgaatga 14280 caaagtttgt atgcagtctc tgcctttagg gactttactg ctaatggggg attgtttatc 14340 aaacctaaat aatatccttg agggaatatt actatactgt gggtattagt gttcttaacc 14400 aatatgggtg ttgactttgt ctgtgtctct ctctgtgtgt gtacaacgtt tgggagtcaa 14460 ttctgtcttt ccaccacatg ggtcctggct atcaaactca gtctctcagc attactggaa 14520 gatgcctttg ccccatgagt catcttgaca gcaaaattgg gtattgagaa cttgaaagtt 14580 tgctgtgtga agaataagta aaacatgcta aaaaaaaaat aaagtagaca ggctgcacag 14640 atggctcagc agttaagagc actggctgtt cttccagagg acccaggttc atttcccagc 14700 acctacatgg cagctcacaa ctgtctgcaa cttctgttcc aggggatcca actccttcac 14760 acatgcaggc aaaaacacca atgtacatac aataaaaata aataagttat ataaaaatag 14820 gcaatctcat agttttacca tgttaattat atgttaaact agcaatgtgc atatattgca 14880 ttaaataaaa atgattcctt aactaaatta atttctttaa ttaaataaaa tgcaaaaaaa 14940 tgaatgaaat aaaatcactt tcacaaggtg gagaaacact ttgggaagac atccagatgt 15000 gaacctcagg cctccacata cacaaacatt aataagtatg ttaattaaaa ttaatttcac 15060 tttactttta aaaatagaac tactagattg tttaggatag aatatataac ttctgcccaa 15120 tatttgctca tattcttttg gaaaatatgt gttcaaatcc tttgcttatt cttaaattag 15180 attgcttgct tttattgttg tatttttttc tatcccaaat agccaaatag tatatttgtt 15240 tggaggtgta tgtggctgtt ttaagacagg atttcttttt ctttttcttt tttttttttt 15300 tagctctggt tatcctggaa ctcactctat agggcagatt ggcctcaaat tcacagagat 15360 ctgcttgctt ctgcctcctg catgctaggg ttaaaggatt gcactggtgc tgggcatgac 15420 aacccccccc cccctttttt tttaaccttc actggtatag tacaaatatg gcacacagac 15480 cattcaccca tacacataag agaaggacta aacaaatatg tttaaacatt aaaaaaaaaa 15540 aaactattgg aaacggaatc gtcaaactgg aatggtggag atttcatcag aactggcttt 15600 gttcattttc ctgtctccgt caaactgtct gctctgtcac agtctggttc tccaccacgt 15660 ggctgctgct tctgttggtt cagatccctg ctccgcagtc tgagccaatg aaatcccggc 15720 tcttcacaaa ggcggtttta gtggcaagtc tttggggctt cttctgttct cttggaagaa 15780 tggccccagc agcttcttcc atagcgcttc tggtgaacca gatggcatct tgttcaacct 15840 cttactgcac tgagcacgca gcctcccctt acaagattca taccaatgtc tcattgtttt 15900 ccaaagtaac ttgagacttg aactttccca gactctcaaa taaaaccatt ccttgtggaa 15960 gagtgcggtg gtctccagtc tttagagact gcctgcctac ctaagcttta ggggacagag 16020 tgggacaaca ctaacaaggt gtgtgggcat gacaacctct aatcttctca gctagcttca 16080 cctggaatct cagtcttatg agtaatctgg actaacagta agggactcat tatttgatgc 16140 ttgccacatc tggggtcaaa tcttggcccc tagaaatggg agatggatgg acaaagatgt 16200 cctatacctt tagctatacc ttctaagaat tcagcctcaa cagcttggag acagacggga 16260 ggacaaacag ggtgggcttc cctggttgct gggcccgctt agggcttggc tgtaggctga 16320 aagggaaggg agcttgctct ctcggccaca cactgtactt gcatctccat cgctgggatg 16380 cagacagtga agagaacaga ccgtagctct gcttgttctg agacatagga aagcgctcca 16440 acaaatgttt cttcacctat cgtatgctct gaggacaatt tccacagact ctagatgatc 16500 atcgttgcac aatttttagg aatcttcgca gtttccttga agtgtgtcct caagagttcc 16560 tgatgctgcc gttatagaag tgggagtagt tgggggtttt atagacaaac tctaaaacta 16620 aaagaaggag atatacacag ttacagaagt gtttggtcag caaggagtca aaaaattagc 16680 cagtgcacac taggcagcat attaaagtat ataatatagt atatattata tactatatat 16740 attataaagt aatgtattac aaagtaatat attactatat attataaagt aatatactat 16800 aattaatata taagtaatat actataacac atataatata taacaaatat aacatataac 16860 atgatatatt atattatatt atagtatagt atagtatagt atatataata agatactact 16920 ggctactaat ccatccaaat ctagaatgaa ccctgtgtaa acacacagga aaacagtggg 16980 aaatctagta gaaaactgga caataggtat taataggaaa ttcacagaag gcaaaacttt 17040 tcaaaatgac aagttcatga atggggcttt atcactatta aatcctttaa aatgcattta 17100 aatgccaagg cgatggctca gctgttaaag agcttgtggg gggatcagga ggaccagaga 17160 ttggatccta gtatctgggc caatgtcaga aggatgtggc agcctgccta taatgctagc 17220 tttggaaggt ggagtcaggg tatccccaga acaagctggc tcgtgggatt agccacattg 17280 gtaagctctg ggtttaattg atagagcctg aatcaatgaa gggaggagag agtgacacaa 17340 agagactcct gatgtcaacc tcaggactct cttcatgtat gtggacacat gtgcatgcac 17400 ccacacacac gtgtgtccac atatttgaaa acagatattt acatacaata cacacacaca 17460 cacacacaca cacacacaca cacacacatt caaagtaagt aagtgcataa aaatggttac 17520 attgtagcag tttgtatatg cttggcccaa gggaagtggc actattagga ggtgtggcct 17580 tgttggagga agtgcatcac tgtgggggtg ggctttgagg ttcttttttc cattttctct 17640 atttgatttg attactacag ccatgaaatt atgatgatct acatatacag attttatgac 17700 aagaaaagaa aaattcataa aaattttgaa gctggagcag tgattctgtt ggtaagatgc 17760 ttgtaagctc tgggctcata tccacttatc agtgagtgca tatcatgtgt gtccttttgt 17820 gattgggtta cctcactcag gatgatgcct tccaggtcca tccatttgcc taagaaattt 17880 cataaattca ttatttttaa tggctgagta gtactccatt gtgtaaatgt accatatttt 17940 ctgtatccat tcctctgttg aaggctttga ggttctatgt ttaagctcta ctaagtgcag 18000 aagaaagctc tcctggttac ctttggatca aaatgtagac ctctcagctc cttctctagt 18060 accatgtctg cctgcatgcc gccatgcttc ctgccatgat gataatggac tgaacctctg 18120 aacctgtaag ccagtctcaa tgaaatgttg tccttcataa gagttgcctt ggtcatggag 18180 tctcttcaca gcagtaaaac ccaacaaata tcactaagca atttttaaaa ataaagctaa 18240 gggctagtga gacggcttgg cggttaggaa tgcccgctgc caagcctgaa gacttgatga 18300 gtttagtttc cagaatttat atggtggaag gagagaacaa actcttagaa ctgtcttctg 18360 accttaacac acactgtgtg ctacactcac gcctacacaa atgcatgccc acgtaaacaa 18420 atagatgcga aagtcaatgt aaatacaatt ttttaaaaaa aaatgaataa catgcagtta 18480 agatgaggtc atattatact ctcaccattg gacaaaactt atatgttatt gtcctcattt 18540 ggttctgttg ctgggataaa atgcagacca gaactaacct gtggatgaag gggttcattc 18600 tgtttatagt ttatcctcct tcactgaggg aaggaaaggt ctacatcacc cagaggaaga 18660 actgaatgaa gcaaagacca tggaggaatg atgcttactg gcttgccccc aggctcaggg 18720 tcaacgacct ttctcatgcc tcccatagcc acctgcccag ggatggccca gctcacaatg 18780 gcctgggtcc tgtcacatca atcaacaatc aagaaccttg cctcacagac atgcccacag 18840 gccatccctc aaccaagatc tctccctccc acatgactac agtttgtgtg aagttggtga 18900 aaaccagcta gcagtttatc aaatcttggt gggaaaaaga tagagagaca gaactacgct 18960 ttgaggagca acttggtggt actaagtgaa attaagtata tataattatg gctaaggaaa 19020 taacattctt gggttaatac cttagaacat cttccataaa caaactagag gaaggtaatt 19080 taaagcacaa ataggcaact aataccatct taaactaacc tacacaccta cgggttatga 19140 atggtgacca caggctctgt atgtctacta agaaataact atgtgagtca aaatgaaaga 19200 accaatcgat ctacatacgc cagcaaggac ggtttctaac tgtgcttcgg atcgtaaaga 19260 taacaaagca aaattagatg atagcatatt aaaggttatg taacatgtga atataatggt 19320 ttctgtggtt cattgttaca tccgtttatg aatgaagctg tggttggtga atggtatgca 19380 ttaggataat aagcgtgggt acctggtaag agaagagatg aaaatggctg gaggaggcag 19440 tgattacagt tccattcaga agggaatcta gagcctggga gatggcttgg taaagggctt 19500 gccaagcaag catgaggatc gaagttcaaa tccccagtcc ccgtgtaaaa agctaggcag 19560 agcaacacat gctcagagcc gaggagtcag agacaggcag atctctggag tgcactagtc 19620 agctgactca gctgagtccc tgcactccag gtttagggag acactgtctc aaacaaacaa 19680 acaaacaaac aaacaaaaca gctggaatgt atgctgactt agttttactt gacatagcta 19740 gagtcacggg agaagaggaa acccccaatt gtgaaaatgc ccccactaga ctgacccatg 19800 ggaaagcctg tagtgaattt tcttagtgac tgctgtgaga aggcccagcc cagcatgctg 19860 tgagtgggtt gtcctgggtg ctgtaagaaa gcagactgaa caagcatcgg gaggcaaaag 19920 ctgataagtt gtcccttcct gtctccaggt tccttccctg atgtccttca ctgatggagt 19980 gtatctgaga gttgtaggct gaagtcaacc ctttcctccc caagtagaca ggaggttaga 20040 aagatagccc tgtgggtcag agtgggtgcg gtacaaatag gaggatgcac cttcatatct 20100 caacctttgc atgagcatct gggtgtagac acacttatgc ctgtgacacc tccttggagg 20160 tggtgatcgt gggactgact ggccaccagc ttagtttcag gttcaataag ctaacctgtc 20220 tcagggaagt aagatgagga gtgagagagt ctggaacacc ttctctggca tttgcacgca 20280 catatggata cctctccaca tgcttataca gtacatacat acactccact ggcatgcaca 20340 cgtacacatg cacaccaaca acaaaaaagt aagttgaaaa aaattaaaga aacactccat 20400 gttaacttat ggcctttaca tgcatacata tatatgtgca cacgcacctg catacactta 20460 cacagaggga gagattacat aggcgttgtc agcagtgtgc tgcagacaaa gaggtatgat 20520 cagtctttct gagatgaatc ttagtgatga ggattccatg atgtatgagc aacagggctg 20580 ggcagtgcag ccagctggtg ctgcatctcc tttcagtttg gattatctta ttgggtcaca 20640 ccagaaccag ggagataaat agtcaagaca gactttgaaa attcatacac cacacacaca 20700 cacacacaca cacacacaca cacacacaca cacacaccaa taaagctagg tttgaatgaa 20760 ttacttttgt aaaatccagg tctgagttgt attctgaaag ctttacttgt ttggactggc 20820 cagtagctca gtcaggcaaa aagacaccac acgggagggc agactttaaa gaagaacgtt 20880 tcaatgaggc aagctgtcac ggcttgtgtg aattacacaa tgaaagggaa aataaaataa 20940 aactatggcg ggctaaaatc tgtaagttct gacatgtggt cctcgtggca ccatctgctc 21000 atgaaagcat gaagaggctt ctggaacttt ccaggactgg gttgatttgt ctccacaaag 21060 ctgcctgggt aagaccctgt ggcactgggt catcaagaag gtactagcag acttcctttt 21120 tatctcagta tttttctacg tcagtcccca aaccatgaaa caacagggct taccctgcca 21180 caccaaaggt caaactatgt tttctcttac ccaactattg cctgggatgg ctccaacttt 21240 attactaccc cacctccaac ccgtaataac tctccagctc tcacttcaaa tccaagagag 21300 tgtggactga gtgaggggac cagagacagg tgcatctggt agaaagttat actcaattaa 21360 ctaactgaat gcatttgcat gtggttttaa ttccactgtg cagcaatttc ctgagtaaaa 21420 atggagagat tatatctagt ttttgagtca ctgtaagaat taagtaaacg tagctctcaa 21480 tctatatctg cacattattt ttcctcgtgg tcctagattt ctcttgttcc tgaccatttg 21540 tcccgtcttg gaatttcccc agccttcaga ggtgtagccc tataatttac cgtccagatt 21600 gcaatccctc ttcatggcat gaattaagtc ttatctcacc aggaagtgct ctttcctttc 21660 ttctgactcc tctccctctg gctttcctct tcatttcttc tctacactgt ttagggaaag 21720 aggtttctta gcaaatcctc agacacccca actagtgggc ttcccggaaa gtctcagccg 21780 tgtgtgggag gtctgccctg aagaagttct ttgccagaaa atgaagcaag gtgttctggc 21840 tggacagtga gggtggtaga ctgtaataag tatgtctggc tttaactctt ctatattcat 21900 ctgaggggag catctgagcc caacacaaca tctccatcgt caatggagaa tcctactcag 21960 ctgacaaggc ccttgcttct gtgttgaagg attacagcaa tggcctcctg tttattgacc 22020 tcatacttag acccatgagg catgcatatc atagtttatt tctcctactt agcaactgaa 22080 aatactaaag ggagtcacag agtctgactg ctctcacggt ctgtatctat tgcttaatcg 22140 aaagcttccc tgttaccctc ccatagcaaa taagggtcca tcccacaaga cactgtggaa 22200 acgcctcctc tcaagctcct ccagacctcg cagtgagtca atagagagca gaggggtctt 22260 acgtctctgg tgcctaaaga ggatggggtg ggcgttaggt ctcagcaaga acatctcttc 22320 ttatgtggcc agcggagagc ccagaaatct taagcttttt cataagggtt tctttgagac 22380 gcttcctaac ctagtgatcc ggttatcacc aagctaaggg aaaacgatca cgtgacacga 22440 aggatgaagg aaaaccctca gattcctcct cctctgaatc tgggagtgga aaactgagga 22500 gctgagctca gagttccaga gccacgcgaa gctgggctca gcagtgtttg cagctgcagg 22560 tctggtgagc ctgtggaaag agagaagggt gagagaagag agtctccaaa aagctgcccg 22620 aattagagaa acaagagacc ctgcctcaaa aaggcagaca cctcaagtgg tcctctgact 22680 tctgttcatg tgtcatgaca cgagcatccc tgaatccaca tacacagctg agaagcgtgt 22740 acacacacac acacacacac acacacacac acacacacag agagagagag agagagagag 22800 agagagacag agacagagac agagagacag agagacagac aaagagacaa agagacaaag 22860 agagagaaac agagagtggc tgaagggagg gggcagggga aggagggagg gggaggggga 22920 gagacaaata atttataaaa ggaaaatcat ttaaaacatt tcagggctgc cgagatgacg 22980 cagttgcaag caggaagccc tgagtttgga gccccagaac gcatccaaaa gccaggtgtg 23040 gagcgtgtgt gttctgtatc tcagctctgg aaacaagggc ctcgccatct attgccaatg 23100 ggctgccaaa ccaacaagga gcgagcaagc cccacttttc acgtaaaata cctcaaagag 23160 aaaagcaaca gatgcaaagg ggagagaatc tcgcttttgc ccagatcatg tagcagcccc 23220 gctcttagag gacgtactcc atgtctatgt gacttcaact ttcaattcta caaaaagggt 23280 tggtttcatc cctctcttct caatctggaa acagttcgtt ttaacgacag atgggtgata 23340 ttacacttag aatcaaactg ttaagccatg tgaatgaatt tatggtcacg tcatttcgaa 23400 gcgtcccctc tacaaccccc ctccccccgc tgggaaaaga ggggcttccc gctttcccag 23460 gaagggaagc tgtgggctct gctgggggac acacactgag ggactttcat cagcatccct 23520 cccaagaggg aagggacatg tctgtgggcc ttatggtctt caaagggacg taaagccgtt 23580 gcattctgat tagtttgtgg agggtacttg tttcaatcca gctacagttt ggtttgtgca 23640 gctggtatct gctgagcagc tgccgggacc tgtgtactct cgaaatcggt gaggctaacc 23700 cctgagaagc agagggtcgg gtgtcacgag agacttttct ttcccttttt ggcagccata 23760 ttgaaagatt cacagacacg ctcttaactt atccatgcaa agtgagactc tgctcacatt 23820 ccctgatact ttccttcaaa atgcaccagg aaatgtcagg aaataattca attagtctca 23880 ggaaatttac aaactgtgca aaacgttttg aatccacttt tagaacactt tcaatgacca 23940 caaaagctga agaaagggca gaaagaggca agggaaagag gaaaggaaga aaggaaacaa 24000 acaagcaaac aaatccagtt tgtcttcaga aggattatgt tggaggttcg ttttgcttgt 24060 agagactagt tcatctattc caggcttgct ttgaattccc tgtataatgg agacaggccc 24120 tcaacttctg gtccttcggc ttctgtgtgg cattacagtt gagtgctatc acacttaatt 24180 atgtcagagg cgtttttgtt tgtttgtttg tgtttatgtt ttactttagc agtggctgga 24240 gttggggtgg ggttgggtta ctccagcagc tcccaagagg atcagccttt caggctgtcg 24300 ctggaagggc agctatatag atgctgcaag aaaagcaact agctcctgct cttagagaac 24360 ttttgtttat tattattatt aatttacttt atttacattt aaaatgttag cccctttcct 24420 ggtttcccct tcgaagaccc cctatgccct ctcccctccc cctgatcacc aacccaccca 24480 cacctgcttc ctggccctgg cattccccta cactggggcg ctgagccttc actggaacaa 24540 gggcctctcc tcccattgat gactgactag accatcctct gctacatatg cagctggagc 24600 catgagtccc accatgtgta ctctttggtt ggtgatttag tccctgtctg ggggtactgg 24660 ttagctcata ttgtttttcc tcctatgggg ctgcaaatct cttcagctcc ttgggtactt 24720 tctctagctg cttcattggg gaccctgtgc tcagcccaat ggtaggcttc gaggaaccac 24780 ctctgtattt gtcaggcact ggtggagcat ctcaggagac agctatataa ggctcctgtc 24840 agtaaggtct tgtactcagc tattaaaaag aatacattta tgaaattctt aagcaaatgg 24900 atgaaactag aaaatattat cttgagtgag gtaacccaac cacaaaagaa cacacaaata 24960 cgtggtatgc actcactgat aagtggatat taggccagaa gcttggaata cccaagatac 25020 cattcacaaa ccacatgaaa ctcaagaaga aggaagacca aagtgtggat tctttgatcc 25080 ttagaagggg gaacaaaata cccatgaaag gagttacaga gacaacgtgt ggaacagata 25140 ctgaaggaat gaccatccag agactgcccc acctggggat ccatcccata tacagtcacc 25200 aaacccagac acttttgtgg atgccaacaa gtgcttatgg aaccttttat ctatagagaa 25260 ggtttctcag ctttggtagc attaattaat tcgttgtcat ggaaaggtgc cgtttacagg 25320 catcccttcg cctctccctg tagttgccag aaacatctta gatatggatc aatatcttct 25380 ggggccagga gatattccca aattgagaac cattggtcag cactctgtct atcatgactg 25440 caaacaaatt gaaaacttga caaacattaa tgaaaacttg ataaacatta atgaaaagaa 25500 actcagaaaa cactttgata gtattttctt taagacatgc catgcgtggt ggcgcatgcc 25560 tttaatccca gcactcggga ggcagaggca ggcggatttc tgagtttgag gccagcctgg 25620 tctacagagt gagttccagg acagccagag ctacacagag aaaccctgtc tcaaaaaaaa 25680 aaaaaaaaaa agacagggtc tcatgaaacc cagactggcc ttgaactcac tatgtagctg 25740 tactgcctgg gtttgtgtgt caacttgaca caagctggag ttatcacaga gaaaggtgcc 25800 ttccttgagg aaatgcctcc atgagatcta gctgtaaggc atttttttca attagtgatc 25860 aagggtggga gggcccattg tgggtggtgc tggactaacc tgggctggta gtcctggatt 25920 ctgtaagaaa gcaagttgag caagccaggg gaagcaagcc agtaagtaac acccctccat 25980 ggcctctgca tcagctcctg cttcctgacc tgcttgagtt ccagtcctga cttcctttgg 26040 tgatcaacag cagtatggaa gtgtaagctg aataaacccc ttcctcccca atttgcttct 26100 tggttatgat gtttgtgcag gaatagataa cctgactaag acagtagcca acgatgatgc 26160 tgagcctctg attctcctgc ctctaccttc tgagtactgg atttacataa gtgagctgtc 26220 acaccagttt atatggtgct ggaatcaaac cacaaaggct cccagcatgc aaggcaagca 26280 ttctctcaac tgagctacag cctcagtttt agcaatgcaa agaagaggaa aaggagggag 26340 aagatatata tatttttcat tacatatatc tcacatatat tttacattac aaatatctca 26400 catatatttt aaagcgctgt agtggtgaga tattgggctt tgtagacacc aggcattctg 26460 ctgtcctggt tcttcaggct ttatgatctt gaacaccata gttctatttg gtgagtctgt 26520 tttctcagga gttgttgact atgcctttag tccagtgcta tctgaagaat gaagatagta 26580 tctctctcag tacacctttg gcaagggaag ataccatctg cccagtatca tcatgtacat 26640 tggttgagag ttaactctgt gctaagtgtt gtggtaaatg cattatttct tattccatcc 26700 tcatggcaat cctaccaaat gtcaaaaatt attatccaca atttgtagat aagaaaacca 26760 aaactttgac acattaaatg actgtcttag gattttgttg ctgggaagag acaccatgac 26820 caaccaaggc aactgttaca aagggtaaac atttaattgt ttctctgagc acttctgtct 26880 tccaggattc taccacaatg gcctttctgc tctatttaca gtattcaact gtttttttag 26940 tacaaagttc caaggtcgtc cacagtcctg caaaaagcaa cacaatcagg tacttcccag 27000 caacggcgat tcttgagtat cgatttctgt attatttaat cttctgttgc tgtgataaaa 27060 tgccacgact aaggcaattt acagcagtaa gagtttattt gggcttacat ttgcagaggg 27120 ataagcgtct ctcatggtag ggagattgga aggcagtgag tggggcctga cagctggaat 27180 aggaagcaga gtgatcacat cctttactgt gaacaggaag cagagacagc aaacagaaaa 27240 tgggctcaag ctaagaaaag aactctcaag tccagccgcc agggatgcgc ttccttcagc 27300 aaagatgcta gctctccaac agcatccacc atctggggaa ccaagtgttc aaacacacgg 27360 gtctgtgagg aatatgcttg accttaacaa agctagggaa atgtgtagac tcctctacac 27420 agctggaaga tgttcactct ctcttctttc ctttaaacag tttcttaaaa attatataac 27480 ccactcatat tttcctaggt gttacaatca taccttacct ttaatcacat tttcatttat 27540 ttgtttgttt gagattttat gtttaattat gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 27600 gtgtgtctgt gactttgtgc acatgaatgt ggtgcccatg ggagccacaa gaaggcatca 27660 gatcatttgg agctgggaaa gcagagccgg aggcagggac atctggcaga gttcagagtg 27720 gacatgacta gactgagctg ggtcatgtgg ggagatgtgc agaggaaggg aaaggggaga 27780 gatgggaaca aggtgcggca ggcaagagag caaagataca cacacacaca cacacacaca 27840 cacacacaca cacaccacat atgtacatat acacatacac acatgcacgc acgcacacac 27900 acacatattt agatgcattg aaatcatttg atatacaaat agaccatgca aagatcaagt 27960 cagtgtgatg gacatttcca gctgtttaaa ctttttaata cttgttagta ttgagagtcc 28020 cacccccacc ccatttcact gctttacaaa gatacaaaac attgttctgg gcatgttggg 28080 tgcattcctt aatcctagtg atgcagaggc aggcagatct cggtaagttg aaggctatcc 28140 tggtctatac agtgcattcc acgctagcta gggctaccag tgagattgtt tcaaaaaaca 28200 aaagtctgta gttatcttcc tatattgttt attcttctct gccttcctct ttttctgcac 28260 acctgtcttc tctgacactg gtcagcatgt gtgagtgctt gggcccgtta atttgtgtga 28320 gcagacatgt gtcaacatca cttagaggaa aggtggcaga ctcattaaca cacacgatta 28380 acatgattag gaccagtttg aaagcagcag ttctccatct tcctaattca ggcctgactg 28440 tgtcaggact ctacactctc tacactgttt tctcagctaa cttgtccttc ctgtctctca 28500 cctcttcccc acactgacac acatgtatac acagcccccc caccacatac ctaccccccc 28560 cctacaccac agacttccag agagagggag gtggtgacta cgctgtatgt gtttaactgt 28620 tacctgtctc tccttagttc tgaatgaaat ggaatctaat cttaatcctt tcctgtcatt 28680 attagattca tggagtccta acggccagag tctctagatc ctgtcttatg aaattcaact 28740 ctcatacaaa attcaaagta tcagtttcag agaaagttat ttgaaaaaaa aaactgtgga 28800 gttgaaagca attgaggctt attctggtcc tcttactcag tgtgtgtgtg tgtgtgtttc 28860 tgtgtatgaa tgcatgtatg catggggtat acatgcatgt atgcatgtgt gttcatgttt 28920 ggtgtatgca tgtgtgtatg tgtgaggtat gtgtatgttt gtgaggtgtg tgtattgtgt 28980 gcatatgtga gcacatgtgt gtgtgtgtgt gtgtgtgtgt gtgtatgcat tcagagacag 29040 agtgtgctca gcttccagta gatcctcagc taggaggtgg tatcttgtga gactcttccc 29100 tctgcatgct gaactgttga ccggcttgac ctgtgaaggt cttgtgcagg caaccacagc 29160 tgctgagagt tcatgcatgc aatagtcttc atgtttattg tagttctccc taactctggt 29220 tcttaccatt tttctttcta gtctgtgatg ttctctggat cttgcaggac aggccataat 29280 gcagataccg agttttattg atgtcaatgg tccactgggc tagcagcctt actgactctg 29340 ggatagatgc tgttaaatca atgacagtgg ctttttaagt tacatccaga cgttaggcag 29400 gaatagataa ctcagatata acacgaaggc ttacccctgt gtttttgatt ttaaaaaatt 29460 gtgagtgtgt tatacgcagg catggttatg cacattgatt tgtgtggttg tgggatctta 29520 tgtcagaggg acttagatgt cttcctttat tactaccgat gttattcttt tgggacagga 29580 acctggactt catttctttt tgccaggaag tcagaaagcc ccagggatcc tcccatctct 29640 tccttcttaa tttctgggct tctgtaggca tgagcaaaac tatgcctggc ttgttaggtc 29700 ggcactggta tctgaacctt tgcttccatg gttgtacagc aagtgttctt aactcatgag 29760 gtcccgattt ttttgtttta cttaatgcta tgttgtactg tattatatta tgttttgctc 29820 tctctctctc tctctctctc tctctctctc tctctatata tatatatata tatatatata 29880 gactggcttg caccatggag tatgtgtcag tcaggagata ccttttggaa gacagtgcct 29940 ttaaaccatt gaaccatttt tctgcccatc acaaattctt tggactaaaa aataagagtt 30000 tctttttcct taacaggaca aaactttatt ctctgatatt taaaatataa gagaatattt 30060 caaaatttca aaaaccagtc ccttaagtta aatcaagtgc ctgctccttc acatctctct 30120 tctcttttga tgtaagcact tattgatcta aactcacttc ttgaaaactc ggagcatctc 30180 acaggcttcg ctatgttgcc tctctacttc catttgcctc aagataattt ttaatttctc 30240 ttttaactat ttcattagcc tattgattat tgaggaccat gtcaaatttc ctaggttttg 30300 tgcccccctc ctcaccggag actttgaaag aatgtatgtg ctgcaaatga caacttagtt 30360 tcttgaagtt tattaatttc tttatgcata tgtatatttt gcatttattt tcactgtttt 30420 gctcaatgtt tgtcattgtt ctagtttcct ttctgttgat aaaacactct gaaaagcaac 30480 tttgaagaga aaaggacttg tttagtttgc tcttccaggt cacagtccat tactgagaga 30540 agtcaaggta agaactctag gcaggatgcg ggagccgtga gggagtgttg tttgctggct 30600 gactctctgg aatgttcaaa ctagttttat tatatagttt gagcctgcta gtctagggat 30660 ggtgctgccc acagtgggct gggtcctccc acatcaatca atcatgacat tttctatgag 30720 acacggccac aggacaatat gatcaaagag atctctcaat tgaaaactga aaaaacttcc 30780 tcttcaggtg aatctacgtt gtgtcaaggt gacaacttgt ccagtagtac taagcagggc 30840 agtcatcttt aatcagagag atgacagtat atattccctt actgtatggg tttgtgttga 30900 gacacctata atggcagaca gattaagtct tcctgcatct gctcagagcc cctgtccaaa 30960 cagttccttc ttccctcttg tctctccttc ttcagacagc ctacacaatt cataagcagg 31020 agaaaacatc cataggatgt tttccagtta gtgttctaga atttcagtag tagaaaggat 31080 ataaaaatta cttagaaaca gggagtactc aatggcaaag tgcttgcctg gcatgcatga 31140 agccttgggt ttatcacccc cccccccact cttagtccat atttatcatc tttcatttaa 31200 ggaagcttaa aataccagag gactagagag atggcttatt ggtaaagagc acttgttgct 31260 cctgcagagg accagggttt ggtttccagc acccacaagg tggctcacaa ccatcagtaa 31320 ctctagtcca ggatgtttga catctttttc tggcctctgt gggtactagg cgcacacata 31380 gggcatatac atacatgcag gccaaacaca aaagtaaaaa taaaaaatat tgtctaaaag 31440 ctatctcaga cacagggaag tgcagagatt tatcctaaac cacactgttg gttaatgtga 31500 aagtcaagct aagataaata aataaataag taaattaatt aattaattaa taagtaatag 31560 agggttggag agatggctca gctatttagg gtgcctgcag ctaggatgca tgttttgttt 31620 ccaggggatt gggggcatga gagaaacctt acaacctgct acagggaatt ggacatgctc 31680 tcatggcctg tgaggatact tatacacaga agacattctc tctctctctc tctctctctc 31740 tctcactctc tctctctttc tctttctctt tctctttctc tttctctttc tctttctctt 31800 tctctttctc tttctctttc tctgagacaa acacacacac acacacacac acacacacac 31860 acacacaaat aaaaacaaat cttggcttat ctgtgcatcc tttaatttca tcactaggaa 31920 ggaagacaga tctttacaag ttcagggtca gcctgatcta caaatcatgt tttagatcaa 31980 caaaggttat gttatgagac cctatcctgt ctcagagaaa caaaacaaac aataacaacc 32040 gctcccccac tcttatggta aaatgaaaag ttactaaagg taggacaccc aagagcacac 32100 gggacgagga tttctaggca agcatctttg tgcagtgttg cacctattat tgaccagcat 32160 tgtttcagga aatgttaggc agaatcaccc cgctgttctc ggaagcaaaa cagaggtgtc 32220 acaccttctg attagactgg ccagcttgct tttcaaatca tctcttcttt atcatcaaag 32280 agagaaggaa acctcggaac aatgctgtct ctgcaatgct atccccactc gatcttatcc 32340 aaggctgccg tctttgttgc catagccacg gagacctgat gcgcaggtat ttgatcttgc 32400 tatgtgccac tgaagggtgg atattccatc ggagaccaaa gaacaggagg ttgttaagtg 32460 tgttgtaggg aatcagagcc atggagaagt tgtttgatct gagtccaggg ctgagcagga 32520 cattggtaca tcctggatga aagctttacg gtttgtacac agcttggaga agtttaagtc 32580 ttggaaaagc cgggcagatc ccatgtgtta actcctccat tgctgccagc ctttccaagc 32640 ctgtttcact taacaaatat ttattgagcg aggtcattac ctccaatttt cactacggag 32700 acttgaaaat taagacacgt actctgcctt tgaggacctt tgaggcacac agatgtaaac 32760 tggccattgg ataatatcat acatgacacg tctgagacac gtgaggggaa agtagaaaag 32820 ggtctatgtc tcatacagca aatgtataaa gttctgtagg taaagtgcca tttgacatgg 32880 ctcctaaaag tccccaaaga tagatttatt tatttattta tttatttatt tgcacatgaa 32940 taatcaactt gttgaaggac tgagagacca cagaaaaaca agcaggagga atagcgacca 33000 tccaggaaag aggcttgact gggcagaagg gtcaaactcc aggagacaga agacaatttg 33060 aattaagatt agccaatctc agagattcct tatgaatggc ttgatgataa ctcctcagtc 33120 ttagccagtg ggaagttttc taggaggaat tctgattggg cagatcttta gccacatgca 33180 atgcccgagg gtagaagatg tgtcttctta ttctgggcag ccacactgtc agctgaaaat 33240 agggtgtgtg ggttgtcatg tggacagtgg cagttcctat aatccatgcc tacctgtgct 33300 gtagctgcac tccttgactt acacacacac actctctctc tgtttctccc ttcctccccc 33360 ctctctccct cccttccctc tctccttttg cagaggtggg tattgaaccc ccgacctcgc 33420 acgttatgat agacaggcac gtatccctgg gcgctatgct gtactattaa cgatttgtat 33480 ccctttcctc tggagaggag atggcgggct tttcaaaaga aagaaagaaa tattcttact 33540 ctatctatag tacccaacag tgtctggtac acctacatac acagatatgc agggcccgtg 33600 gaaacacaac aattccactc ctgtttaggc aacttttcat gctctttttt tccctctgaa 33660 cttggcttca tacagttttt agtgttcaac cttagtcttg ttaaatccac tccaccatag 33720 ctcagcctaa tcatcggctt ttgaatggat ttacagaact agacacacat ttcctcctac 33780 agagcagacc ttacgtccca tcagaaagca gttggttaca cccaaatgtt gtatggccct 33840 ccattaaaaa agatgagctt atttgtttta tgtgtgtgag tgttttgctt gtatgtatac 33900 atgtataact tgtgcatgcc tgatgtctgt tcaggtcagg agcaggtgtt tgctggatct 33960 cctggaactg gagatataga tactatgacc tgctatgtgg gtgctgggaa tcaaatccaa 34020 gttctctgca agagcagcag gtgttctttt tttaagttaa tattttagag aatttcatac 34080 acaaatactg ttatttacct cattaccacc ccactcaacc tggatcctac ccccaaccac 34140 tcccttctaa aatttatgac ttcttcattt cttttttgag atttatttat ttattttata 34200 tgcctaggtg cttctatcta tgcacccctt acacggctga tgctcaaaga ggccagcaga 34260 gggcgccagg tcccctggaa ctggagttag agatgacggt ggcttttcct ccctttttac 34320 caggagacct agtcctcaca gaggttttca ggtcagttcc agtttgattc cttcaagtcc 34380 tgtgtccaaa gtgtgtgttg tctttagcca tacgcgttta atgtcaagtt ctgggaggca 34440 ccaaagagaa cagcagaagc cgctgggact tctctgacca acagcacgga ggtttttcat 34500 acacggtact agatagtttt tggctcttgc agggagcatt atagcccaag ctggtgtaac 34560 accatttaaa gcacacgtaa gcagtatatg catatactta cacttgtatt catttatgta 34620 ttatgcatgt gttcttttta gagatctatt ataggtgtat gaatgttttg cccacatccg 34680 tgtacatgca ggacatgtgt atctgatgcc tgagagagtt agaggagagg gcatcaggtc 34740 ccctggaact ggatttacag acagttgtga gcagccacga gagtgatgga gactgagcct 34800 gtgtcctcta caagagcagt tactgctttt aaccactcag cctcgccccg ctacgcgtgg 34860 atttttaagt aaacacgaaa taaacttttc ctttcgcctt ttcaaactca ctttgtctta 34920 tttatgcctc ttccctcttt tccctctatt ttattcttcc ccctcccatt aaagccccac 34980 ccccttttct attcccccct ccctttttgc taggacacag caagtgctct taactgagga 35040 gcagtctctc tagaaccccc gcccacgcca cacatctttt agagctgaaa atttcctaca 35100 gcttccgaaa ggaagaatca ggatagagtt tagaaatata accaacagag gagaagatgg 35160 caaaactact gctgccttca gcacagagag gctttaccta gaaaatgggg ggggaaatgg 35220 aaacatggaa aaatgcattg attggaaata taccgaggac atgggaagtt ttaagggtga 35280 tgagcttgag gcgaggaaac cagatttgta cagttttgga agaaacatgc aaagtttaaa 35340 ttcggctggg gatcagaggc agttatttta tggcaaagat gagattggtt ttgtccagct 35400 tggcctcatt tgtacctctc cattttagct atagtcacat cttattaata cttacctcac 35460 tctttggagc ttaggcagaa aatccattat ttatggttga gtattcacca tttatgtgta 35520 agtaaggaca tctgtatgcc attgaatatg tggttgtgaa gtaattaaaa cagtttacag 35580 tgtaaaatga aagacatttc tctattttaa acatgcaata gagaaatggc attttatata 35640 ttgtttagaa agatttattt atttatttaa tgtatgtggg tacacactgt agctgtacag 35700 atggttgtga gccttcatct ggttgttggg aattgaattt aggacctctg ctcgctccag 35760 ccaaccccat ttgctctggt tggccccact tgctccatcc caaagattta tctattatta 35820 taaataagta cactgtagct gtcttcagac acaccagaag agggcgtcag atctcattat 35880 ggatggttgt gagccaccat gtggttgctg ggatttgaac ttaggacctt cggatgagca 35940 gtcagtgctc ttacctgctg agtgctgagt catctcacca gcattttata ttctaactag 36000 attcaataga cacttttgtt ttgttttgtt ttgtttgttt tttgtttgtg atgaagaata 36060 ctaaggggtt tttacttagt tagcagtaag caagatgaac tgaatatatt gtatcaagag 36120 gcatcgagat tgcatggctg taactcaggt ggtgatgaag gaggataacc atcccctgga 36180 ggctaacctg agctacacag caagacccaa cccaaaagca gccagccaac caaaccaaac 36240 tcaaacccaa aggcccgtga aaaattgaat tctgatgatt agtgccccat aaggacctta 36300 tggaacatga aaacaatagg aagaacaaag agaacgagca ggtcagaacc acacttaccc 36360 aaattaaagt ctcccagctt ggaagatcaa atcatacatt acctcaagta actcatattt 36420 gtctgcaagg tgtcagctga ccccttttct ttaatatctc acttcccacc cccaccccct 36480 tttaaacaga aagacctttg attgtggtgg ccaataattt gaagttttga agaattaatt 36540 ggccctatta tacatgaaga catagtaatc ccccttcact tttggaagcc tcggagagag 36600 tttgagaatt tcaagttgaa atatcacccc ctttcacaaa attctgtatt taaggatagt 36660 atttttcaga atttaaaaaa attttttact ggaaacttaa tattacagca ttccccttca 36720 gggtttatgg tcaagctttt ttaaatatat gttttatttc tttaactggc agatgtttga 36780 aagatgagag taggattaat cctcatatta atttttaaat acatctcatt cttccaaaga 36840 atgccgagaa aaagaaccaa ctctccatgt agtaactggc aaggaaggaa atagggctcc 36900 taccatggca actccctgtc agggatgctt agtgttgttt ctgtccaggt tcctcactgc 36960 tgagaacaaa tagccaacaa tactgagaga cggggaggtt tcttctggct aacagtttca 37020 gggatacagt tattgcagga gaggagagga gaggagagga gaggagagga gaggagagga 37080 gaggagagga gaggagagga gaggagagga gaggagagga ggcagctggt taaaccgggt 37140 ctgcagtcag gaagcagggg agccacacat aaatgccgag actacacttg ctttttcctt 37200 ttgctccact gtgagaacac agcagacctc aaaacacagc agtttgggaa ccaacacaca 37260 cgtctctttg tgtctggcaa ttgatgtaag ctccatggtt tttgcttttt ttctagctcc 37320 tttaattgat tgattgattg attgattgat tgattgattt ctgagactgg attttgctct 37380 gtaagccagg cttgtgtaga actccaattt tggctggcct ggaattggag aagttcatat 37440 tctcattata cagagtgctt aatggcaccg ttatgctcag gtcattcatt cattcattca 37500 ttcattcatg catgcatgca tgcatgcatg catgagatag gagttttctg tgtagctcag 37560 aatttccctc caacttcaaa ttctccttcc tcagctttcc tggcaatgaa aatacaggcc 37620 tgcgccatta tgcgcaagtt atattggctg ttgttaatat ttatgttagg aaaagaaaat 37680 agtctctgtt ttacatacaa tctacttgga atcatttttt ttttttaaag ttaagtcggg 37740 gtgaggggtg tgtgcaggtt aacaagcaaa cgcaattcct ccagctaacg ccaaggggca 37800 ccctctcctt cccctcggtt tctggagcgc cttgagttct ttccatctgc acaaagagcc 37860 cggcctcctc ccgggtgaca tcacaaaggg tcgttctccg ccccttcggc gccaccacgt 37920 gtgtccccga gctgagtggc catccgactc agtccctcgc cggccggtct aggcagcgga 37980 ggaggcgagt gcttgatagt ggctggaagc ttcgctatgg gaagtcgctc tttagcactg 38040 tcgcggctag ccctgctctc tggttctccg cagccgctgt cgttggagag caccgggagg 38100 cgcgggttgc gagcgcgcct gcttctcacc gcccgggcgc cagcgcccct gggcaggtgc 38160 tgagcgcctt tcggagcctc ccctgctgcc tccctcttcc gcctgggtgc ctggctgctg 38220 cagtgcacat gggctgctgg aggtagatgg gctcaccgcc cgtgaggcgg cggtggatgc 38280 ggcgctgggc agaaacagcc accaattcca gctgccgtgg ggccgagcgc cccgggcgcc 38340 gctgcgagcc ccgagctcgg ccatggggac ccgggcaagc agcatcaccg ccctcgcctc 38400 ttgcagccgc accgccggcc aagtcggagc cacgatggtc gccggctctc ttctcctggt 38460 gagcgatagg aggggaccct agggtggcgg ctacgttcga gcctgtgtcc gggagccggg 38520 cgggtcctcg ggcccgtagt gccggaggcg cgcggtggga ccagacgggt tggaagggtt 38580 ggttacaagg caatagccag accgaggagg actcctacta gatcgtgtgt gtgtgtgtgt 38640 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgg tgagctaagg ctagtgagtg ttaaagaatt 38700 atttttcttg caatctggct gcttgaagga ggcgcgagag gtgtccgcct cgaggtcctg 38760 cgcatttttg ggtttttgcc cccgactcgg cctatctcgc accccaacac ccagcctcac 38820 cccaatatca ttcgctgcca gaccctcggc gcggtgcgag gcccgagtag tcctgtcagt 38880 agccagctgc cggttgtggc cgctcctttg tcgctactgc aatgtcatgt ccggatgcta 38940 ccgcagtgga gacagccgga tcggtgcgct ccggacgcga gcaaccgctg catgcttccg 39000 cgcgcgaagc tttccggagt agggcgcagg ccaccccttg cttcagcttc cttgcttctc 39060 tcctccctct tagttcagat gggtcctgat ttctgcaagc caaaagaaga agaagaagaa 39120 ggagcccggg tgggtggtgg gggtggggtg ggagggggag gaggagaaga aggacccaga 39180 gacccaggcg cctctaaagc acaggcttgg agccaaccgg cgctgtggct ctcagtcgag 39240 ggggagggga aagagctaca taaaggacga ttaattttcc ccatcctttt cttatttctc 39300 cctttctgtg tttagacatg tcaccaagca agatgatgca ggctctctca gcacccaaat 39360 ctggggcgtg cccggattgc aatcctccat cactttatct gatcaaaacc tgacatcatc 39420 ccttatccaa aacttacccc cagctggccc tttatttcca atattggggc caagtgggga 39480 attaggtgga agaaaatttg aattcaaacc cttgagatgc tataaaccaa tcatgtcttt 39540 catccctgca gggaggatga aggttgttag ctaaaaacta aacaaaaaca aaaaagagct 39600 tgttttcatg gttgggctgt gtaagggaag gcaggcctct tgtctccttc ctctaaaggg 39660 cttactgcct ggccagccaa gatctactgc ttccatccca aggcatgaca gttgctatgg 39720 cctgggaatt cattctacag acattctggt tctctctgtc tctctctgtc tctctctctg 39780 gctgactctc tttctgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg 39840 tgtgtgtgtg tgtgtttagg gagagagaga gagagagaga gagagagaga gagagagaga 39900 gagagagaga gggctaaaga aagtttgttg ttcttagcat ttatgaggta aatctcagtg 39960 tcccttgaga gacgcttttc tgtcttttta taacaatggc aaagtccaag ccctttaaac 40020 aagtgtggat agtaaataat cagagagtct ttgggaccgt ggacttgaat tgacagtctt 40080 agattgaacg agttcaagga agcactgatc ctaatgacct gagttaggtc ccttgctgag 40140 ttccctccaa aggtcttaga cctgcttccg tgatgagcat atactgccgt gtgtccctgc 40200 tccacacaga tacaatcctt atacaatact tatagatcag tccactcaaa tccaatctaa 40260 agttagtgct caatgtatag tcgtaatctg gaattatttt ttaactttta gcctgatata 40320 taggtccttg aaggtctcag catagtaaaa ccatatgctt taagtaacca agaaagggcc 40380 tagttctttt tattaaatga tcaaactcaa tgtacccaca gattgcaccc agatttgcaa 40440 ctgatgacct acagtataag tttgacagtt tgatgaagtt tatgaaaggt attttagaga 40500 caatctcaag gtgacgatgc tacagggttc cttgatttcc tggtaactat ggcaaaaggg 40560 ctttctttaa attgatggag taggaggagg gtgagctgac catcctggag tgtgggaata 40620 ctccaggaaa catcaggagc tatgcaatga ccttctccat ccccactttg gacggtccag 40680 tcatcaggac gggccctgtt tctgtataca acagtgatct ggtgactctg ttctcaggag 40740 aattggtccc attggtaatc ttggccacat taacagcaga caccaggtag gacagctcaa 40800 gtaaacatgg tgtgaggtac ctagaagaat aatgggggat acaggaaggt taatgctatg 40860 tatatacagg gtggttagat aaagaaataa tgatggcttc caattgccct tctctcattt 40920 ttttttcata gcagtctttg ttgttttttg tggctctttt ttttttctat cagcatgaag 40980 agcttactat aggctctgat atgtatgcct atcatatgaa cttgtcaaaa gagcagcgag 41040 ctaatttata cagaatatgc cactatccct gtcaaacggg tgtcacctcc attctacgga 41100 tgagaaaata gtctccaaga atcttactgg tctgttgtag tatgtggtga ctctgggctg 41160 gaactccttg gctcctactc tctctcaccc acgtaccaca ctgcccgttt cacaacttca 41220 agttgtttgg tgagtgtaag gacagagaag gactgctggc aggtagctga gacggtggct 41280 tgattaattg gggaatataa gtgagacgtt cacaaggacc tttggagtga gtcagtctag 41340 ttgctggcat ttctattgct gatgtgttga tgtgaccgtg tcatgtttgg aagttactca 41400 caaactgaaa tgcagcatac acgttccagg ccaaagttct gtaaatgaga catttagatc 41460 tgtagtggtt tagggattgt cctagaagga cttgcttact gcaatgctgg atgcagtcag 41520 aggtgcctct tgcctttagg gagcttggtt tgactataca gatagactga ttgcaaggga 41580 tggtcaaggg aggaaagcaa cttccttggt aaagactttg gatttggagc tttggaaact 41640 gctgttatcc acggactgag aattcctgca ctgaaggcac agggaagaag gcatagcaga 41700 agaaggagtg gaaagattct aaaagccaga ggaacagaag ttgctgtgag atcgtgtcac 41760 ctggaaatgt ccgggaagct tcactcatga agtttcaaca gcatggctgc gtaagcggga 41820 tctgatcaga gaacaccagt ggacaggcta acacagaagg gggaacttca tagggcctca 41880 gccctagaca aaaaattaga aatatgggct actaaggaat atatatgtgt gtgtacaata 41940 cgtgcatata tatacaaaca tatgcacaca cacatatatg tatgtgaatg taacacatgt 42000 gcatatgtat tatgcacatg tgcacatatg taatagcaat tgaaaataac gccatgaatt 42060 tgagtcgtgc aaggctggta catgagaggt tggctgtcga gaggaaaggg aaggaagaaa 42120 agatttccac acaagaggtt gtccagctgt ctggccatcc cttttctagg cagctggcat 42180 ctcaccattt ctggtcacca aaagaaaaaa taccttattt atttatttat ttatttttct 42240 tttgccggtg ggaaatggtc agacacatca tcagacacat catcagacac atcatcttgc 42300 ttagagggtc ccaagtgtta ctgttggcca ctgctcctga gttctacctt ctgacttcca 42360 actatcacag aaaatggcta tctgcctggg caggagtgta catgtgaggt catctggggt 42420 ggggtggggc actcaagtcc tagccttggt gacaaatcca attagaatat gaaatcagag 42480 ctggcgggca tacagagggc tctctccctt tgatatactt taacacagca tcctagactg 42540 tggaagctgg gagggaagct aagctgttac tgatctaaca ccccacacag cttggctgag 42600 ggaaagaaca gcctccactg cggaagtgac tatttttcat tttaccaaat ctacatggtg 42660 cagaggcggg atcctaagca gtggaagccc agtgatgaag tgggattaga accttgcgtg 42720 gcatgaagct catttctgtg gcttataaaa caagtggccg gtgtcaatgg atcaatgggt 42780 gagcatgcta gaccccgaga gagacacaga tgtgatgttc acatcttcaa aatatgtagt 42840 ctgtcagagg aggaaaacac ttctattttg gaaaactttc caatcttgga atgatatatg 42900 ctagttgatt tatgggaaat tgttaatgag gaggaacaag taatccagat cttctactgg 42960 attgaacaac tcctgatttt taacccataa gttatcccca caaagtttag agtagggtca 43020 agctttctca tttggactta ctgtatatct ctggctatca gatttagtca cagacagaca 43080 gacagataga cagatagaca gagtctccag gacaactaag caattaagat gaatctacaa 43140 gaaaaacctc tgtagttaca cgggcacagg tccactgact acttatacat ctggacaatg 43200 tctaccaatt agataaagca accatagttt gccttcccca gtggtcaggg gtgggtttgg 43260 cttcagagct gggtctagtg agtagctggt gtcttctctt tggaatttgg ggacatcaca 43320 tcttgacttt tgcacttgac tccatcttga ggagaggcaa agcttcttgg gaggcttggt 43380 agaaaagctg caaattggct ctgtgctaac tcagccagca ggcaaactat aattggctta 43440 cataaagttc acgattatta ttattatttt tttttctttt ggtgacggga aacggtgaga 43500 tgcatcatca gactattcaa gggccaggac gaattgagcg acttttcctt gcgtaaacag 43560 ctctgcctat tttccagaaa gcactaatac tctgttggct tgcttccccc ctgcacaggc 43620 ctggcaaatt gcggtaatgc caggtcacca ccagcaactt cttggcaatc tcaggtgctt 43680 ctcttctgtg actagaaggg cccagattaa ctggtgggta tccggtggtc atttggagtt 43740 gagtccattt ggagcacgag atgcagatat attggatgga cagaatgaca actgaagcca 43800 ttagtaggag cagcttggcc cactgagtgt cagcaggagg ctggacttgg ttgtttctgc 43860 attagaggcc actgagttgt gttctttagg aatagaggcg agagatggtg gataaacact 43920 ggctttctcc agtgtttata tttgtcaaat acctaaaaat caacaggatc ttaaattggc 43980 ttgaaggctg ccacctggtt ggacagtgat tattaacctg gagggagact ggcagatgat 44040 cagaaatatg ggtggggcct ctgcttatgg agagaggttt tgatgaccgt gctgttgaca 44100 gaaggcagat gcgcaatcaa cattcatgaa gagtgctgaa gtcggggtcg tgtgtgtcct 44160 ggttggaggc aacatgtcta tagtgttagg aaagttgtgc tcagggggct ctcaggaagg 44220 tgtgggatgc agcaggacct cggtaagctg gcaggatgct gccccatgtc ctttgatctt 44280 ctcttcagaa tggttgcaga ctatgcatga ctcgtcgcag gtctaagaca aataaaaaga 44340 attggagaga agcacatagc caaatcagaa cacggctagt agagctatga gacaggccta 44400 tatcgtgttc cattcgctct ctgcaaccag gatcactgta gtggctctgt tgcttggcac 44460 catttgcaaa atctgcttgg agggaagagg aacacaggct taatagagtt agacaagaac 44520 atggagcttg tccatgtgcc ctggcctcaa gagagcagct gtgaaatcag cagagctcct 44580 gtgttagtgg cctttggagg aggactaagc aaatggctgc taacccttta tattgggatg 44640 ttatttaatt tatatattat aaaaatatta tgtaatatat tcattcatat actatagaaa 44700 tggttcttat tactgcattg aatatcttct gtaaagcact acagtgtaca ttttacaagt 44760 cccagacatg acagctttag tcccatttta cagaatccag aataaggttt gatagagttt 44820 ttgctcagat atctgcccat tattacgttc tggagagagg gatgaaatct agctcagcat 44880 agtttcaaac acttagctct ctctgccaag agagaatgga acaagtgaac agaatggttc 44940 acttcaactt cccatacata cgtttgttta ttctaaaggg aatggtttta cccaagaggt 45000 gaacatgccc gttttattct ctgagtttca cagtggtacc tgaatggttt gccaaaacta 45060 ctggtacttg tgagcatggt gtgtgggagc tgaaagggga gggcgtggag acattgggcg 45120 aacactttcc tatggtgtga ctctgacgag ccatggtgcc acggttgaga tggaaagaca 45180 ccccagcaaa ttgaaacttt ataaataggg aacgggcgtg ttggctctcc tgcagccctg 45240 ggtcactttg aaaaatattt gcttaatgag taacagcgag gtgtgggctc cactctgctg 45300 tgtagttagt ttctgctcct ttagtctgag cacctgagga cctgctaggg tctttgctgc 45360 tagcctgact ctttgtatag gataatagct gtaccagcaa tgactagcat ctgtccacat 45420 ttcattccgg ttctcattcc atggtttgag tggttcgtgc taggaatgta gtggataggt 45480 tgccacagtt actggagcac cctcataaac aagccgttag agaaaaaagg agagagcttc 45540 atacatgcat aggatacgtt ttgatcaaat ccacagccct tcactctctc tctccccttt 45600 caacttctcc caacagcccc ctctccccat cacctttccc tcccaatttc acgtgctctt 45660 tctctaaatc cactaagtcc gtgcacctgt aggtacgtga gtgtaggact atctactgcc 45720 gcatggatag cctgtcagga gccccatccc caaagaaaat ggagtcttcc tccccgaaca 45780 gccatcaatt cctaacggct tctcagctag gggtaagaat ttatgactcc atctcctgcc 45840 cacgcaggct tttgactggc ttggttttat acaggtcttg tgcatgcagt cccaaccatg 45900 cacacttcat gtgtgcaggg ccctggcatt tctagcaaat actgtttcac tctaatcaaa 45960 tgatcctttt ggatcttaca agttttctcc tcttctttga gatccctgag ccttgtgggg 46020 gaggggctga gatgcatcgg tctcattttg cacctaacac tccaaggtat tctaggtatt 46080 ctattctctg tccgttgacc aggcgtaggt ctctctctct ctcttttttt ttttttttga 46140 ttcccatctt ctacactgtt ggaaggggag ggagctatgc tgattaaatg agctttgaga 46200 tctgttgaaa tcccaatttg tagccttagc agacaaaagc tggaaggaaa tgatgtatta 46260 gtgccagaaa agagattgga gttctggtct cccaaaaacc tttatgacaa gaccactgtc 46320 tgatttcact gcagacatgg ctagctagct ctgctcagca aggactcaga gaggcagcgg 46380 ggtacgaacg agacaaggaa acttgcaggc aaagatgaca ggttttcaga acagtccaac 46440 ttgtatgttt gtgcttattt tggccccact tttctttcca taattatttc cctttctcct 46500 acaggggtat atttgtgttt ttcatcaact tactaaagcg taaagactat ctactaagga 46560 acaggcatgg ctagggtgtg ggtctataga agtgactaca ggttatctgt agtctcccct 46620 tggagttcgc agaaaacgtg atgtttcatg gtaatcatca taaagcagaa gggagtggat 46680 ttcagtggaa gggaccggta tgtagaatag ccccagtgta ggacagagtc tgtgtcttca 46740 tctctgacca ctatgactgg gaagaggcag tgggactgtg tgtcgtggga cccttgaaac 46800 tatgaagatt cgttcagtgc aggagaagca gggtaataac atggcttgaa acattctttt 46860 tttttaaacc ttatgtatct ccagatctca catctccaga aagttcctgg cagtcttgag 46920 acactaatat gggttctggg aaccaaatgc aggcctctgg aagaacagca agtgcttaac 46980 cactgagcat cactcccacc tgccaacatt gaaaaaagaa gttttaatta atccttagag 47040 aattccattt agtgtatttt gattccattg ctcctgaagc tcctcccaga tcctccccac 47100 cgcttaactt acccaagttt ctctccctct cgcgcctcct tgcattgagt atagtttgtg 47160 ttggctaact gcttttgggt atcaggccac aatcaataag gaaaaaacaa aacaaaacaa 47220 acaaacaaac ctgtttctca tactgaggat ggaacctagg gttttcacat attgagcaag 47280 tgctctacca ctgagctgta ctcagctctc agatcgctcc agaagcctgc tggaggatgg 47340 aagctggagg tcagctgctt tcacagggtg gagatcacag tgccggggat agtggcggtg 47400 aagatgaatt ttctagaatt tactggtggt aagatgggag ggggtcaggt ggtctcaaag 47460 gaggcggact cctaggtgcc taaattgaag tctgtgattg gaaggatggg ctgggggcgt 47520 ggcgggtaga attagaaccc taagcttctg ctgtgtagtg agttaagttt gaagtgtcgg 47580 cgatgaacat tggcttgggc cttgattttg ccattttgaa atagctgact ctctgcaggt 47640 ccgaagcgca ctgagccatc gcgactgtcg ccaggcattt gggctgccgt ccttcctccg 47700 cagcatccag cagcacagac atctgtgtgt agagcaatgc tctgttcccc cttaaggttg 47760 cttcctctga ttgcattccc caaagtggca ttgccaggtc agaggaaacg aatgagtggt 47820 ttttatggcg ttcgctgcag attgccagat tgttttttag gaagacaatc aattcagcca 47880 ccagccacct ataaatgtgc tttctagtcc atgaatctgc cgcgacggga ctttctcatt 47940 tcaaattacg tatcttcttt ttaattcagc gatgtaagga cctttggtgg tctctgtgac 48000 agtttagtca tttctagggg cataataaac ttacttgcat gatgcttgac ttttaatgac 48060 tcatggtcct tctagcgagt agaagttatg ctaatcttat ttttatcaat tctacttatc 48120 attctggtta taggttctta ctagctgtaa tggcattgct ttatattcta ttgacttttt 48180 acgatcatgt ttacgtattt gctttgtttg tgtgcacgtg ggcatctctg tgtcacagtg 48240 catggaggtc agagacaatt tatggagtct gtgggtcctg gggatagaat tctctacctg 48300 tcaggcttag tgttaagccc cattgcccac tgatccattg caccaatact gcaccactac 48360 tgtactctac tataatatca tgaaaaaaat aggattgtgt tattaaacct tttcctttct 48420 gggtctgaga atattctttt tgttttgttt tttggatttt tttttttttt tttttttttt 48480 tttttttgcc cattttgtta ggaattcatc ctttgacagg tagttataaa gaatcctaat 48540 tgctttgaat tttataatgt tattggttga cttgttgata tttctggata ttggtgtcgt 48600 atatgtggtg ggaatcccct tggtgtgttc tgaaggctat atgtgcgtgt gtgtgtgtgt 48660 gtgtgtgtgt gtgtgtgtgt gtgtgtaatg gattctgtgg gtaaacacac aggggagtgc 48720 tgggcccagc tgattcacca tttcaggctg ttcttccgtg gagtcacctg ggacaggtga 48780 tagctgatat attaattgta cccgttcagg agaagatata cggagtattt tctgaactct 48840 agactagggc atcagagact ttttctgtaa agtgctgggc agtacggtta tcttatgata 48900 tctgtggtaa ctacttaatt ccacctttgt gcaagacatg tgcagacaat gtctcgaaga 48960 gtgagtctgg ctgtgtttca ataacacttt atttgtggat aatgaaattt gagtttcaca 49020 cagttttcac tggtgctata ttgctactca ttcattcctc ctcatttccc agctgtcaga 49080 ctataaaact gttgtaggcc tgcctatagg ctatacaaaa atagacggcg atagagtcca 49140 gcctgctttt agaccagggc attaatattg tgtgagagca aattggggca actcactttg 49200 gaaaaccctt acttaatcgt gttctgggca tctaaagtga gattattaat tgtaaatcct 49260 ttctatttta ttattatttt ttgttataag ccaaggttta tttcatttta attaattaac 49320 ttactttgag gtagtgtctc tggttgacta ggctgaccca aggctaacct tgaattgcct 49380 ccgttttaag tgctgggatt atggtgtgtg ctggtctcgg aactcaccag gcaggcgctc 49440 aaatagctga gccagccaca tccccagaat cccaagtgtt tgcttgtttg tttttaatat 49500 agtgtgaaaa gtagccttgc tttctcttgt ctttatcttg acgtctgctt taaaacaaaa 49560 cttctacagt gtgctctgtg ttcggggaag tgagtttcag gattataatt ttttattttt 49620 taacagtatt attattatta ttggtatttt tgagacagag ttgctctgtg tagccctggc 49680 tgtcctggaa ctcactttgt agaccaggct ggccctaaac tcagaaatcc gcctgcctct 49740 gcctcccaag tgctgggatt aaaggcgtgc gccaccacgc ctgcataatc ttttattaaa 49800 ttaaataaaa gaaataacca atttttggtg tttgaggaag aatatcccta tggaaatcag 49860 cttgaatagg aaatcagtgt ctagcacaag ctggccttgg attttgtgat gttcccgtct 49920 ttccatccta aattctgtgg tcataagtat ggaccaccat gtaaataggc aagtaccact 49980 agtacatagg catgcatcca gatggcagtt cttttcttat ttgcttgttc tgagccagtt 50040 tgggtgtgga tccctgcctc actttgaata cactatgtat atccatctgg ccttgaactc 50100 actaagtaca ccaagctggc tttgaactca ctgcatacct caagctggcc ctgaacttgg 50160 gatattcctc atgcctttgt tcccaaagtg ctgggactac agtggatgcc actgtgatgg 50220 agttgggtta cacctttaaa caaaaaagtc tagagtttgg aggctggaaa tgtaacctgc 50280 ctagcacaca gacaaccttg gacttaattg tagcatgaca aaacaaaaca aaacaaaaca 50340 aacaacaaca aaaacaaaac aaacagcaac aacaaaaaca aaacaaaaca aacagcaaca 50400 aaacaaaaca aaaaaggaaa catttctcaa atttatactt aaaatagaat tgaaatgttt 50460 taaaacattg tctattcaca ggtaataaac ttaaagtcta gtaaaataat aaattggtgg 50520 ggtgataatt tcccaaactg gtattccatc cagcaattac catgtcagac atgttctcca 50580 tactccttct gtgtagctaa ttatcaaaac aacttagaat ggccgttgtt attgctgtgt 50640 ccattttaca agcgaggagg tagaggcttg gacgttggct ccttcccagg ttagaaagat 50700 agaaaaaaaa aatgtaattt tcttcagctt aggaaccaga ttcaccagct gacactcaca 50760 gggtgtcttc aattcacacc cgaccctatt tgcttcaaga tagctaccgg ttggaaggct 50820 agattccaca agacggactc taacggacgt caggtatcaa gagtaaaagt caggttataa 50880 cctgtgcttt ctgttgtctg actgtaagtc agagttccta tggctctcag ggggttcagt 50940 taacttgtaa gcatggcctc acatatctca gggaaacact ttctgaatat ggctctcttc 51000 tacagaaagg agacccatga taaagcatgc agaggaaggc atgcttaggg cagccatata 51060 aggagcaccg tggagctgtg gagctactgt gtctgtctgc cttggacacc gtgagctctc 51120 tgaaccttgt cctttcagag ttttatggag atgctatttc ataggcacaa tttactacgt 51180 cattagacac tggtgactaa acttaacctt caacccctct cctcatccca gagattgagg 51240 tagaactgaa agtagcaagc tgaatataaa aaccttgtgt gttctgacca ctggtctcca 51300 tcttggtggc accctctcag agcctccgtc ccctgtcatt ttatcaccac ccagaagacc 51360 ctaatcacgc tggctgtgag cttccctact ttgtaggatc catgggaaga gaccacatct 51420 atattcctta ttatcacagg atcgcggcca gcagatacct gggcgagcag ggcgtatgtg 51480 gtacattcta ggcttaaatg gtttttcctt atactttgaa gcctcagttg ctgcactggc 51540 ctgggttgta tgctcttcca gacatgccca gtcagcatga agttgcatcc ttccatcatc 51600 tactgttttc tatgacatac tatttcactt attactcact gggcatccta ttggcatctc 51660 tcagtaagca gagaataaag aaattactac tgtgttggag ggagacgggg ctgtggtcca 51720 gtgactgatt gctgtatgca ctttctcttc tgcattgcgg gttccagaga aaacagctcc 51780 taggtgaaat cttccctgtt tttttcctct ccagtgtccc ctgagcttgc ctccttctgt 51840 tcctacagtc agcacattct ggctctggca ttctctatag tcaccagctt gaaaaacagt 51900 ccctgcttcc tttgctttgg caataggaga cggctctccc tccttcccat tcctctcatc 51960 cctcccatcc tcctcttcct tcatctctcc ctctcttcca tccttccttt cttccttcct 52020 ctttccttct tttcagagaa ggcagtagaa atgaaagagc caaccaagtt tatataacat 52080 aactggaaat accctgtctg tgatttgtgg aacctttgtc cttgaaacgt ttgtttgtat 52140 gtatcccacc tgccccacaa cttcagatct ggggaattat agccatccag tgtttatatt 52200 acaaattaaa aatgatggcc cagctatctg gtcaacaatt ctgagggctg agcagctctg 52260 tctttgtcat caggtatcat atttcatagt catcctcata ggcaaacctg attaccttac 52320 aatctctaca gtaactacac acacacacac acacacacac acacacacac acacacacat 52380 atatgtatat acacacacac atacatgtgt gtgtgtgtgt gtgtgtgtat ggaaaaattc 52440 tatatcaaag tccagagcaa ttttcctgct tcttactaag gaaaagcagg tccacctctg 52500 tcttaagttt gctcgtttta taatgaaatg ttactagaac ttgagaggaa atgaaagtaa 52560 tgtctgcctg gtgcagggac gatggctcag tgggtaagat gctggcttac aaatgtgaag 52620 agatttgagt ttgaatcctt agcactcatg taaaaaggct ggatgtggtc ttgtgagcac 52680 ctgtaacccc agcattatgg aggctggaga caaggaggct tgctggagct tgctggctgc 52740 cagtcttgtt aaaatggcaa gctccaggtt tgatgagaga gccctgtctc aagtcaacga 52800 agtggagagc gatggagtag gacactctac atcctcctcc ggcttccccg tgggcatgtg 52860 cacctgtaca cacacatgca tataccacac acacacacac acacacacac acacacacac 52920 acacacacac acacgctaac gtcaatggca cagatctgtt gtaaccttta agctaccaag 52980 ggatctcttg tttatctgaa ggatgaggta atggaagtgg aattgctgaa caataagtag 53040 atgtctacat tctgacatgg attcacacat ccgggtgctt cttgtcttca gtcaggccca 53100 cctgtgctgt aaactgcaag tgacacatca taggtgtgct ataggccacc aggatgcaca 53160 gtgagactct ggaagccttc acagtcagtc acctgcacca agtgctgctc tccacttgat 53220 ctctgcttct ctcgtttaag cttctaatca ttcaataaac acttgtagtg tttttggttg 53280 ttgttgtttt taaccaaata cagtttgaga cagcatgctg atacttgatg catctatcca 53340 ctgttgtagt tgacattcct tttttttttt gattagatgt tttctttatt tagatttcaa 53400 atgctatccc taaagttccc tataccctcc ctccgccctg taggtgacat tcttagatac 53460 atggaatttt cataataatc gcagtttaaa cacagagaga atctaacctt cttatattgc 53520 ttataatctt aagcacaaag tgtaatcttc tctttttttc tttcttttaa atgagagaag 53580 aatatttgaa ctctttagta tattagcttt ttattttgag tatcacgaag aagctaattc 53640 ttagaagcca attatagttc tgttaagaga gagtaatggg gggctggaga gaaggctcag 53700 aggttaagag cacaggctgg ccaggcatgg tggcgcacgc ctttaatccc aggacttggg 53760 aggcagaggc aggtggatct ctgagttcga ggccagcctg gtctacagag tgagttccag 53820 gacagccagg gctacacaga gaaaccctgt tctgcattat agattccagg gaagacacac 53880 agagtataag tgtgtaagta agaaaacgat gacaaccata tagggaaaag aagaaaccga 53940 ggagggcttt acaattgggg atgggaggca gggagagcac ccaggaggaa gtcttgtctc 54000 tctggatgat tttgcttttt ctcagacgtc agggggaagt gagagtttga agccgagtgt 54060 gaaggaacca gattgagcgt gtgagttaga gtgggtggag agagagagag agagagagag 54120 aaagagagag agagagagag agagagagag cgagcactct gctcataaag aaacttatgg 54180 gggagaccag gaagtttcta tgagcagctg gtctctgcaa tgtttgtatt tctttagatg 54240 cccatgctgg gagctggcag atttcaccta taaagggcca agtgataaca gtaagctaac 54300 tgaggtttct gggcagaatg gtctctgtca tagctttgtc gctttcccac agtagcctga 54360 aagcagccac gagtgctagc tacatcagtg tctggactct aggaagcttg tttataagac 54420 gagctggtat gttggcattc ctttgctggc ccctggcttg aaggatggtt tgcttattat 54480 tttcctagtg ttcattaggt agttgagcaa gtgaggaagt atagtctgta cagtagtccc 54540 tggccttgag ctgcttataa gcattaagga aagataagta tatacactta cggtactgcc 54600 aaccaacatt aagagacact atgtaattag acgctggtac aatagacaga aacaggaagt 54660 gcgatgggct agttgctcat ctgcttggca tctgggtgtt tagaaagaga tttaaaggct 54720 acataaggat ggcaggattt gacagaatca caacacgcag gagggtagaa ttggttgaat 54780 attactactg agttcaacct gctcatttcc taagtgagca acccaggatc ctgttgactt 54840 cccaacaggt tgggtaagaa catgccttct cttttcaaaa tctttgtcca gttcttttcc 54900 tgactttttc tgcttgatcc acccagggat gtttatgtac gtatgtttgt gtatatgttt 54960 tatattgtta ggggtgagag aatggggtcg ctccaggtta tcgattgcat ttaggatgtc 55020 aaaagactgg gaccagggtg atcgtgtctg ctacttggac tgatgtggag agaagagaag 55080 ctaggtgagg ggtatgggag ggaagaagca gttgtgcaca atagaaaact ataccctgac 55140 ctatatacat gcaagaaagc agtcaatgaa gtcttggtgg gaagaccctc tgaagtaaga 55200 catacccatc ctgttggttg cgtgcctagc ctttaatggc taagccagct ctgtagaata 55260 tgctcattct gttgaagaac tctgaattgt tagagtgaga cagtttgtat gttaaaacag 55320 acaggtcttg gtgatgtaac agaagttagt aaaagagaaa tcacagagca acatggttgg 55380 ctttttttcc ctttgcatac acaaaaccaa ctcaaacaag agaacgggga agctgagaat 55440 ctgtagttcc aaattgctag gactattaca tagatgttta ttgagggggg gtggaagtaa 55500 aatgctaata tcaagttatt taaaaatgcc aggttaggta gagaaggggg tgactctgac 55560 tgtgagattg ggttgtatcc aaaatgtggc tcaggatcaa agaattttac agtcagaggg 55620 gtcttggttt agtgttaggg actcaagagt tagttagata ccagaaacac tcagattgat 55680 taaaaaggca agaaaaaaaa aacctcacag ccaaacaaaa taagttcctc accctgttgt 55740 atactcacaa cgatctcaga gataagacga ggtacatgaa gaaagtactt tcaaaaatca 55800 tctcagacag tattattttt tctttttcca ttttcatgtg tgtgtgtgtg tgtttgcgtg 55860 tgtctgggct ctcatgtgtg gttccatgtg cacatggagc cccaaggttg acattgggag 55920 ccattctcaa ttgctcttcc ttcctatttg gcgaggcagg tgcttaagcc aaacccagag 55980 ctcagtgata tgtgtcatct ctctagcctg tttcttctag ggatccgctg acacctctgt 56040 gatggaatta caggtagact gatgtatcca cttgatacac actcttcccg ggcaatttca 56100 gttttatact caaaagccgg aaactatgtt agatctctct gacctgtctt agccatgtct 56160 atacacatgg tgggggttgg gagaggaatc aagggtctta attggaagga cccagcctcg 56220 gttttcttga ctctcactat ccatgacctt gtctcccctt tcccatttgt ttgtcttctc 56280 ctctagggtt agctttcact ataatctttt tatattgtgc aggggccatc tttaaatatt 56340 gtcttccatt tggtctattt ctgcccagat ccatggggtt agagggaaga gctaatgtgt 56400 gaggtgcaca tatgtgcatg tgtggatgtg cgtgcgtgcg tgcgtatatg tgtgtgaact 56460 atgtatgtat gtatgtatgt atgtagaggc atgttctcag gtagctcaag tgactctcaa 56520 acttgacgtg tagccaaaga tagccttgaa ctcctgatcc tcctacctca gcttctcaag 56580 tgttgggttt acaaacttgc gccatcacaa ggggatattg ttttgttttg ggacaaagtt 56640 tctttatgta gctcagactg gtctggatcc tgtgatcctc ttgtctgagt ctccctagta 56700 ccggcaagta cttccacaac ttggaagggg aaactcttgt ttagatacag tttttccttc 56760 ttcttcttta aatagcacag tgggattagc tattgactct ggaattggga gagactttag 56820 aggaccctgt agtcttgttt gagtaataag ctctgctctc aacaggcctt ccctctgatt 56880 aggctttgca aagcagagct gctctgtttg agacagaagt gagggggctt ggagcctacc 56940 tcccattgct ccttggcaag aggcagccct gtgatttttg tccagttcag cgcttgcttc 57000 ttgtcccact ggtttcttcc tccatccact tcgctaaaca cagttaattc acactctcct 57060 ttattctgtt tgcaggagat agatgaggta attttatagg tcagcattat agtcccttaa 57120 aaactgataa aggagccagg catggttgtg caaactagcc cagctccgtg gctcatacct 57180 gtaatcccag cagcaactgg agagtctgag tcaggatttc cacgaggcca gcctgggtta 57240 taaaataaga ctctatctca aaacaaaata tcttcaaaat aattttaccc ccacgaaatc 57300 aagaatccca aatggagcaa gcatggtata gagtgtaggg aatgtgcagt tgctgggtgg 57360 ggtttgaaca cagctaagtg accccaacaa gtcagacatc aggaaagaac ctaccatccc 57420 tgtcttagaa caaaagtctg gacccttgtc tagtctggtg ggtgttgtca gcaatgtgca 57480 cactcagggt ggctccctgg atcaggagcc ctgggtgggg aaggctgggg ttgaggctcc 57540 atttcctaga gctttgtgta tctccccagg gtgcacactt ttgagtctac agtgggggtg 57600 gggcactaat cttgtactcg ggctccattg gacagatggt ttcctccacc ctgggcatct 57660 gctgaactcc ggatagggtg tctgggctgg ttgtgcaagt agagggagca ggaaaaggaa 57720 gggccaagcc aaacgctgag agaggagact taccaccaag tagcaggatc ttctctttct 57780 caatttccaa tatgaaatta aatttcccaa caagaaaacc aaccactatc catcgccaac 57840 cacctctgcc cctactttca gactcagaag gaagaaaact aagtatatcg taaactctaa 57900 ggaggaaacc tcaagaaccg gtgagaggcg gggaagtacc ctggccccac ataactgtgg 57960 ggttgggaaa agaatagact ggggtcaagt ttcgagatgc taagaagaca gactcttgat 58020 caccttgcaa gcagtttttt ttctttttct tttgctttgt tctttcagtt accgatgtag 58080 ctgtaaatct gtaaatctag aaggaatttg ttcggtagct ggtagcttgg ggcagccatg 58140 attttcatac aaatttctcc atctcactga ggatggcaaa gactatcagg gccttcattt 58200 tgacagcccc agggaagcag tcactctagt cttttgctcc ctgcttctct ttcacagcat 58260 ctcaggactt ggaacttggg atacccccag gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 58320 gtgtgtgtgt gtgtgtgtgt gtggtttgtc cagacagggt gtctccatgt agccctggct 58380 atcttagaac tcaattggta gaccagacta gctccaaact cagagatccg cctgcttctg 58440 cctcccaagt gctgggatta aaggcatatg ctaccacacc caacgggtcc tccaagtttt 58500 taatctggaa gttggtgaaa ctctgacctg ctgggtgaag tttttatttt agaacatgaa 58560 cactgagtta ttcttgctgg acccaagctc aatgggtaga tttctcaatg gatgcctgca 58620 gggattgtgt ctatcggttg tggccttcag agactggaca aactgcgttt gaatctaaaa 58680 gcaccagctc cattgtttaa tgcacataac aggactgtca aaagtatggc tgtttgttaa 58740 aacaaaacaa aacaaaacaa aacaaaacaa aagtttatca tcaggtatgc aggtgctata 58800 gaaatgacaa ctttggcaca cacctccctc tttctgatgt caagttggtg tggtacccgc 58860 cagatcttga acagtggtga tgacctgtta ttgctgttgc tgttgctgtt gctctgttcg 58920 cctctgctcc tctatctgac acctctctta tttctttcag cttggattcc tcagcaccat 58980 cacagcttgg tacattctgg tgccaagtgt ggccattccc ctggctatcg ccttcctctt 59040 cttcttcatc tgtgtgtgcc gcaataacca gaagtcttca tcaggtaccg atttaaatga 59100 tccagtggtc ctgcagagga gagattggga gaatcccggt gtgacacagc tgaacagact 59160 agccgcccac cctccctttg cttcttggag aaacagtgag gaagctagga cagacagacc 59220 aagccagcaa ctcagatctt tgaacgggga gtggagattt gcctggtttc cggcaccaga 59280 agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc 59340 ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtga cctatcccat 59400 tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt 59460 taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa 59520 ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagtcgttt 59580 gccgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat 59640 ggtgctgcgc tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg 59700 cattttccgt gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt 59760 tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg 59820 cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt 59880 cgccagcggc accgcgcctt tcggcggtga aattatcgat gagcgtggtg gttatgccga 59940 tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa 60000 tctctatcgt gcggtggttg aactgcacac cgccgacggc acgctgattg aagcagaagc 60060 ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa 60120 gccgttgctg attcgaggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat 60180 ggatgagcag acgatggtgc aggatatcct gctgatgaag cagaacaact ttaacgccgt 60240 gcgctgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc gctacggcct 60300 gtatgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga atcgtctgac 60360 cgatgatccg cgctggctac cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga 60420 tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc acggcgctaa 60480 tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga 60540 aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga 60600 tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc 60660 tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg 60720 cggtttcgct aaatactggc aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt 60780 ctgggactgg gtggatcagt cgctgattaa atatgatgaa aacggcaacc cgtggtcggc 60840 ttacggcggt gattttggcg atacgccgaa cgatcgccag ttctgtatga acggtctggt 60900 ctttgccgac cgcacgccgc atccagcgct gacggaagca aaacaccagc agcagttttt 60960 ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag 61020 cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg caagcggtga 61080 agtgcctctg gatgtcgctc cacaaggtaa acagttgatt gaactgcctg aactaccgca 61140 gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc 61200 atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag 61260 tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg aaatggattt 61320 ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct ttctttcaca 61380 gatgtggatt ggcgataaaa aacaactgct gacgccgctg cgcgatcagt tcacccgtgc 61440 accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt 61500 cgaacgctgg aaggcggcgg gccattacca ggccgaagca gcgttgttgc agtgcacggc 61560 agatacactt gctgatgcgg tgctgattac gaccgctcac gcgtggcagc atcaggggaa 61620 aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac 61680 cgttgatgtt gaagtggcga gcgatacacc gcatccggcg cggattggcc tgaactgcca 61740 gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag aaaactatcc 61800 cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag acatgtatac 61860 cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg 61920 cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc aacagcaact 61980 gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc tgaatatcga 62040 cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat cggcggaatt 62100 ccagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat aataataacc 62160 gggcaggggg gatctaagct ctagataagt aatgatcata atcagccata tcacatctgt 62220 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat 62280 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa 62340 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 62400 caaactcatc aatgtatctt atcatgtctg gatcccccgg ctagagttta aacactagaa 62460 ctagtggatc ccccgggctc gataactata acggtcctaa ggtagcgaat aacttcgtat 62520 aatgtatgct atacgaagtt atatgcatgg cctccgcgcc gggttttggc gcctcccgcg 62580 ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct 62640 gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa 62700 ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt 62760 ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag 62820 ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag 62880 ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt 62940 cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg 63000 gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt 63060 tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa 63120 tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg 63180 cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag 63240 atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg 63300 gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac 63360 ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc 63420 agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg 63480 ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg 63540 ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa 63600 gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc 63660 accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt 63720 gtccgctaaa ttctggccgt ttttggcttt tttgttagac gtgttgacaa ttaatcatcg 63780 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat gggatcggcc 63840 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc 63900 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg 63960 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag 64020 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc 64080 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat 64140 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 64200 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc 64260 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag 64320 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc 64380 gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc 64440 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata 64500 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc 64560 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac 64620 gagttcttct gaggggatcc gctgtaagtc tgcagaaatt gatgatctat taaacaataa 64680 agatgtccac taaaatggaa gtttttcctg tcatactttg ttaagaaggg tgagaacaga 64740 gtacctacat tttgaatgga aggattggag ctacgggggt gggggtgggg tgggattaga 64800 taaatgcctg ctctttactg aaggctcttt actattgctt tatgataatg tttcatagtt 64860 ggatatcata atttaaacaa gcaaaaccaa attaagggcc agctcattcc tcccactcat 64920 gatctataga tctatagatc tctcgtggga tcattgtttt tctcttgatt cccactttgt 64980 ggttctaagt actgtggttt ccaaatgtgt cagtttcata gcctgaagaa cgagatcagc 65040 agcctctgtt ccacatacac ttcattctca gtattgtttt gccaagttct aattccatca 65100 gacctcgacc tgcagcccct agataacttc gtataatgta tgctatacga agttatgcta 65160 gccacagggg cactgcattc tgggaatcaa cctactggcg gggtgatttc atttcgtttc 65220 tgacttttgt gttttggtgt gtatgtatgt gtttaacaga gtgtatggcc ggtgagtttg 65280 ggttctttct ttctttcttt ctttctttct ttctttcttt ctttctttct ttctttcttt 65340 ctttctttct tccttcctga aagtgaatgt ataaagcctt tacaatgtat aactgttgga 65400 aaatgcccac cactaaattt ttttttaagt tccacatatt ctccattttt gccttcttat 65460 atatatcttc aacactattc tgtgcacttt aaaaacttaa cataaacgca gtgtgacttc 65520 tcccatatgc tgggtcccga gactctcaac ttcttaaaaa cctaatggca tcttgtgact 65580 cctagaagta gacataagtc tttcaacctt cacacctact ctttctgttt taattattat 65640 tgctatttgt cttattgttt gtgctttaca agcgttcttg aggacggagg gaatctacga 65700 ccctgttgat gactgtaact ctattcgact ttgagttgtc ttcttcatgt cttgttatat 65760 agttcatatt catggctgaa acttgaccat actccctagc gctgattgta tggttttcgt 65820 ctggacaccg tacactgcct gataacttgt gcacctctta acgctactat gctctgggct 65880 ggagaatgaa atctttaagt caccaggact tgctgtttca gtggcttgac acctgggcca 65940 ccaaagaact cgatcttcat cttttaggga cacctctgct gcaccttgga aagccaacct 66000 taagtgccag tggcacttta tgcccagctt tgctttgaaa gatatctttc ttgttttttt 66060 tatccttctc tttctctctt ttttttaaaa atacacatag tcaataggtc cagtctgccc 66120 tcaaggcctt gctgggtttt cttcatcatc caatcacttt cattaaaaat ggctgcagct 66180 gtaagaactc ttgtctgata aattttcaac tatgctctca tttatctacc tgccctctga 66240 tgctcagtcg tcagactcta atgcaaaggt ggacgtcggc tgcctttgcg tgggcgggct 66300 tagtggtgag gaactgatat cagaaaaaaa atgccttcaa gtatactaat ttattaataa 66360 atattaggtg tttgttactt gtgtggtttt ggtatattta ctactgaact ttcgcctttc 66420 cctctgctca ggttctagcc ctgttcatct cttggggaag gatgtgtgtt ctgaaaccct 66480 tctcctgtcc aatctgtaaa tcatgaatat tattagcaaa agtccctttt ggagaggtac 66540 ttggaggttc actgcacagg gctctgataa gcactgccaa gcctttccta gtctttcgga 66600 gttaaacctt aggtaacttg atacctgtag gacgatctgc acacattcac aggcttctag 66660 ccaaaacctc actgtccctg acgaactcca gagggcttgg tcagcagtga ggcggatgtg 66720 gtcctgtgtt ggtgggatat ctcaagctca ctgcaggttt tcctaagctt ggaccccacc 66780 tctgcggaat tccaatgatc aaacgctagc aactttaaca tcctcttttt gtgagaacct 66840 ggaagctgat actgtgtata ctgtgacccc ccacgtcaac aactgggact gtcttccggt 66900 ttcactcatc tctgtgtggc agctttcaag atgaacatcc ttgtttccct tccctcttgt 66960 tggtgcatgc ctttagtcca ttggaagaga gaggcaggag gatctttgat tttgaagcta 67020 gcctgggagt ttcaggacag cctggctacg ctaactaaaa aaaaaaaaaa aaaaaaaaaa 67080 aaagatcaca catgcgcaca cacgaacaca cacatacaga gcaacaagaa caaacaagaa 67140 tgggtcagtt atgatggggg aggaaacagg ttttgtcagg ttctcactgt gtcagaagat 67200 ttagaactga gacatacaat ccattcatta attactagta tgttaagttt atagtttatt 67260 agtaatttgg gaatttttta ttaataaccc aaatttgtgg ttttatattt tacaacatat 67320 aaagtttaat tactttattg gatctttaaa tttgtcttaa cgggttccta ttattatgcc 67380 cattttacag atgaaattgg gcttgtaaag ctaagtatat gagatggttt cttatcacta 67440 gaacagagtc cctgaaaagg aagaaaagaa attctttaag ggaggaaacg ttgatcttaa 67500 ctcatggttt ccaaggcttt aagtccatgg ttggctggct ctgttgcctt tggttgatgg 67560 taaagcagaa acctggtaag aaaacacgac ggtggaaggt tgcttacctc ctttcggcca 67620 gcaagcagag agacacagga agagtcttgg gacaggataa ctcttcagtg gcacaggtcc 67680 agtgaccttc tactagctag aaccccctcc ctaccttata gtctattcag ctatgaacac 67740 atccatggtc gaatatacca ctagctgaag cccaaacctc caatagatga gcctttgtgg 67800 gggacactta atatccgaag cataaaacca aggaatgtgt ttaagccctc actgagaagg 67860 ttagcaaggc taggtttgaa tccgtgttgt gtatgttttg ggaagaaggg tagagcgagt 67920 ggctgtaatc taagactgtt cattctcttt ataagatcac tgaccagaaa cctgttgcca 67980 cctgaaacag tgggcagaaa catggcagtg aaatctgtct gggtcatgag taaagttcaa 68040 atgcaagtta agtaagtggt acaaacgtag gcatgagaac taaacattag cattatatgg 68100 tgatcccatg atgcatagct ttcccagaag gcaatgcacc atcagctact ttcttaaagt 68160 acccagagtc aagtgtgatg gatggcacac acctggaacc cagcactcac ttgcaggctg 68220 gaagaccaag gccttagggt cagcctgggg agagggctca gtcagtaaac catttgccac 68280 acaagcctga gaacctttgg atacctggca cagcaaagta cagggctctg tggtgcacac 68340 ctacagtctc cgtgttgggc agacagagac aggaagatcc ctggggcttg ctggctggcc 68400 agtctagctg aactggcaag caccaggctc aggcaagtca ctccatctca aaaaaataag 68460 gtgtaaaatg gtggaagaag gcatccaatg tccaactgca gcctctgcat atgtatgcac 68520 aaggtcatac acacacaaac acagaggagg aggaggacga ggaggaagag aaggaagagg 68580 agggattcaa cgttagcctg ggctacatag taagaccctg tctcaaaaca gccatatagc 68640 tattggagtt atagttcatt gacatttgat caacaacact gcagaaactg ggtgtgacat 68700 tgcatatctc ccaattcttt cactccagag gtgaaggcag gaaagataaa aagttcaaaa 68760 tcatctttgg gtgtatagca agttggagac cagcctggga cacattagat gctgtaaaaa 68820 aaaacaaacc aaacaactga aataaaacaa aaattcagca taaaatacta aaatatgtga 68880 ctttctgatt aaagctaatg atagcaccat ttgtaccata gaaaggtctt ctctggcttg 68940 cgcttcttcc tctgaggagt atagacagct gaaaaggaag gctgctagtg tgagggcaga 69000 ggagacaaga gagcaaccag aagaataaga aacagaagca actgttggga gatccaagcc 69060 atgttatgca ggcaggcagg agaaggagtc tgttcaccaa tttgaaggac taagaaggag 69120 tttgtgaccc ctgggaaaag actacagact cgatccaatt ataatgaaaa gatttgttga 69180 ttagctgcta gctggatgag taatctctga gccaacttcg agattacttc gcagaagggt 69240 tacggggaag gactttaaaa ggagaatatc acacaaagac cttcaatatc taggcaagca 69300 agtaacaact gttctatttt gacaaattaa gtgattgagg cgactcaaaa caatcttggg 69360 ttatctgtgc aacaagttac agaggcaaaa aaaaaaagtt agtcataaca agtgttatgt 69420 atggggagga aggtgtgact gagtcagggt tattggaaat ttatcttccg gggaatggag 69480 ttagagacta gtgggtatgg aaatgaatgc ctagcataaa acggagcttc tttcattttc 69540 ttcagagagt aatatagttc ctgtgggctg ttttaggctg aactcaatta gatagggctg 69600 cttttcaagg agaaactgct tcaaggagag gaaggagctg ggcgagcatc tgtggttctc 69660 caactttcat gtaaataagt cacccgggag tgagcagctt gttctcttta aaaaaggctg 69720 attcccacaa tctgattgga gaggtttgtg gtgaaattca aaaatgaagc ccttgcaggt 69780 ttttggacca cacttagaaa actatcttta gagaatttcc gttcctgcca atggggacaa 69840 gaacagctaa gaggagctgg ttagactcgc accttcagag gcctcctgtc taccctttta 69900 atgtgcaaat gagatacctg aaggtcctgc tcacaggcag actctccagg cctcttctgg 69960 gaaagggcag ctggcatcct gtgttgctgc caagctcttg ggtaatacag gcactgctgg 70020 gtttttcttt ttccccgtga aactgggtag tctgggatga ctttaccttt caggaaaggg 70080 gcagaacaaa cacaggagtc gatcactcat ttcacaggag ggaaggaaac acactttgat 70140 gagctgaaga aacacccaga accaagcaaa tctggtcaaa acttacaact ggttggggct 70200 gggcttggta aaagtgcctg cagcagaagc acggggacag gagttcggat cctcagcatc 70260 catgttagaa aataaggtag gtgcagtgct gcatgcccca tgcatgctcc cagtgctggg 70320 gaggcaggga cagcagaatc ccaggggttt gttggcttgc tagctccagg gtccatgaga 70380 gtctgtgcct aaatgataat aaggtagaaa ggtgatcaag aaagacatag aactttgggc 70440 acacaaagcc tacccctcct ccagcaggat tgtataacag cagcagaaag aaaggagact 70500 tacagaaaga aaggagactc ctcgaagccc aactatttgg catttgtgca gagagctcta 70560 gggatgtagc tttcaagggt ggacttcttc acgagcagtt gtatttgggt tactcatact 70620 tctgtaagta actgcaagaa attcatctgg acgcaagaag catcatttct ttgttccgtt 70680 ggcagtgccc tatctgaggc aaatagacat ttgctgtgtc tctccaggaa aagtcacaca 70740 atacacagtg aggctgcctg gcagaggaat cctgtttatc ttgtggtcaa ccagaataaa 70800 agagacaaga aaggccagag tctcagtata ccctagaaga atatttcccc aggtaatcaa 70860 cttccctcca ttaggctgca cctcttaaag tgtctgctac ttctaaatag taaactggtc 70920 acaaagatta gacacatgtc ctttgggact cacttctgaa ctgtacccct gggctatgaa 70980 ttggtcactt tcctgctgtt atgacaaaat atcaccactc aagacaatct aaggaaaaag 71040 aatttgagct tccacttttg gtgagagagt ccataaagac agggaagaga tggcagctgg 71100 aacaggaagc taagcctcaa tcaagaagct gagaaagtga gctgggagta gtgaggctat 71160 ataacatttc aaagtgatct gcctcctcca gcaaggttgt acctcctcat caaacagtac 71220 tacctacttg gtcccatctt ggttgctttt ctactgctgt gaagatcatg accaaggcag 71280 cttataaaaa aggaagcatt taattgatca atttataaac actttatagg ggactggaaa 71340 ggtggcttag tggttaagag tactggctgt tcctcttcta gaggttctga gttcaattcc 71400 cagcacccca tggcagctca caaccatgta taatgggatc tgataacttc ttttggcctg 71460 aaggcattca tgaagacaga gccctcatat acattaaata aattttatga acagcttata 71520 aaaacgttat aaaacataat ttcagagtta ccctatgact actatggcag gaagcatggt 71580 ggtggcaggc aggcatagcc atggagcagt agctgagtgc ttacatcatg cctgaaagat 71640 gttgacagag tgagacaggg cttttaaaaa caaaaaccac gttccctgtt atgcacctcc 71700 tacaacaggg ccacacctcc taatccttcc ccaaacagtt ccaccaatta gagatcaaga 71760 attcaaacat gcaaatcatg ctcattcaaa ccaccacaga gcctacaagg ctctcattca 71820 aagcaccaca agacagcaag acctatatga atcagacctg aggtgatggc tgtagcttcc 71880 tgagaaaggg tttggggaat ctttccaagg agccagcttt gctctgtccc ttacaagtag 71940 ctgtctcccc agctctctag gatcttccta gagattagat taggatttct cccaaacaaa 72000 cacaaaaaaa gtccaaatgg agagggttca aactcaacat ttaaaaaaag tgagtcacag 72060 taattatgtt ggggctcaaa tatctatttc ttaggataga aatctctcat atttaaggtc 72120 tttgggagga gaaacatgaa aaatttctgg agttgtcatg catacacaca tacacataca 72180 cactatatat atatatgtgt gtgtgtgtat acacatatat acacacacat atatgtatat 72240 acacacatat gtctatatgt atatatacat acatatatac acacacatat atgtatatac 72300 acacatatgt ctatatgtat atatacatac atatatacac acacatatat gtatatacac 72360 acatatctat atgtatatat acatatatac acatataata tttaaatatg ttttatatat 72420 ttatatatta tacataaata tgcttaagta ttatctattt aaatatatat ttaatattta 72480 aaacatatat tataccacac gcagtcctct aaccctccat tggagatgtg cctgttccgt 72540 gttagttagc attctcctac tcagtcaccc tctgcttcat ccactgcata atgtcaaatg 72600 ggagatctca cagagaaaga acatgggaca gcaagttcat tttcaagatc acatgacagt 72660 tatttccata agtatagaca cgtggatttc acatctcgtt tcgtccattt gtttgaatgc 72720 agcatacctg taggacggtg aaaggcagcc tggttcctgg ttgagctaag gctagaaacc 72780 ctactgaccc taaagggatg cttggagttt gcctggttct taggtaccag gcttcatgca 72840 cgaggccaca gccctccata gatttgtggc cgtcagtaac gtaagggcaa caccgcaagc 72900 caaagccccc ccccccacct gccacacaca cacaggtaga ggacgtgacc catggtcacc 72960 tagcctcaag acacatctct attttaatga ggtacctaaa ggcctggagg acttagccaa 73020 taaactctcc ttcccagatg ttccttcctg caaaaaaggt atttaacttc aggccctccc 73080 tgagaagtgg ggtacggttt tactcagcca cctttcacca ttacaataaa tgccttaaaa 73140 tcatggattg ctacttttca ccgggatctg ctatggagaa gaccttcttt gcctacagag 73200 ctaccatcta atttcttgta gaagacctct ctgctctccc agccatggct gccaccaagc 73260 tcaagcttgc tgccacagac agccagagct cctctttttt ccccctcagc cctgggctag 73320 attcagcccg ctggccccca ctctgttctc aggtcttttg tggctctcag tatgcctggg 73380 tgtccaagag cccgagaacc agacagctag ggcctccttg taggccgggg gacccccaag 73440 ccagttctgg ctgtcctggg atctcagact gtgctttccc attctggaag ggttattcca 73500 tggctcccca tagaccaaca ccagcccggt gacagtgtgg ggtaagctct gtcaaaactc 73560 cagcgttctg tctccccaag tagctgtgct ctgtggtggg cacccctaca cacacccatc 73620 cacagaaaca cagatgagtt cttccactta gtctgagaca tctgaacagc tactaggttc 73680 tgggctaggg gctagacgtg gggagaattt gatggagcgt tgaaactgct gatgaggaaa 73740 gagaagctct ctgttcacgg ctctgtcctg gacatccaga gcaaagtata gaaatgtact 73800 tatgctatag gctgaataac tgagtgaatg caagctcctg actgcaagaa gttcccaaca 73860 tagcaagaga cacgaagaga gacattctta taacaatggt aagtgatgag aaatatggaa 73920 acagtaatta attatatcca agcataatgg ttacattcca tagaagcatt tgaagaccca 73980 gacaccaaat acaactcaaa gaaaagtgat ataaaatttt ctagtgtaag cctagtaatt 74040 tcaatgataa gttgcatcaa aagtcaggaa ctgatcaatg cgattctatc ctcttcagag 74100 ctttgaccac gataagaaat agcttgatgc cagcttaaca ttagactcta aagcagtgac 74160 cttcctagtg ctgtgaccct ttaaaacagt tcctcgtgtt aggctgaccc ccagccataa 74220 aattacttcg ctgctaattc ataactgtag ttttgctact gttataaatc ataaggtaaa 74280 tattgatatg caaccctcca gaggggtcgt gacccccacg ggttgagaac cagtgctcta 74340 aagtgaactg caggtgtgct gtgggctcta ccacataaac aaggatgcca tagcttttct 74400 actgttgttg ttccccgttt ccaggcttat cattaaccat catcaggaga tacgaggccc 74460 aggcatgtga cagcttagca gaagacaggc caagtgcaac cttgtgtgga gctcattctc 74520 tgagcaaatg ttcaaaggct ctgggggtgc tcattgctca gtctcacttt gaagcctgta 74580 ctataggaac aggcattatt tccactctgt tgataaggaa tctgagacat gcattgtgat 74640 ggccccagta ttatacagct agcaaagggt ggagggagcc aggaaggaca tttgtagagc 74700 tgagtgactc aaggtcataa aatggcaagt ggcaagtggc aagtgaggtg acttgactca 74760 tacctgcctg gaaccttttg tcttgactca accaaactcc tccttattga ttttttaaaa 74820 aaggtatctt tttaaaaaat gtatgtgcat gttgcactgg ctggttctgt gtggcttgag 74880 ttatcataga gaaagatgcc tcccttgaag aaatgcctcc atgagatcca gctgtaaggc 74940 attttctcaa ttagtgatta agggtgggag ggcccactgt gggtggtgcc atccctgggc 75000 tggtagtcct ggattctatt agaaagaaag gcaggggagc aagccagtaa gcagcaccct 75060 tccatggcat cagctcctgc ttcctgacct gcttgagttc cagtcctgac ttcttttgct 75120 gatgaacagt aatgtggaca tggaagctga ataaaccctt tcctccccaa cttgcttgtt 75180 ggtcctgatg tttgtgcagg aatagaaacc ctgactaaga caaacgtgtc tgtacccgca 75240 tgtgtttatg cttaccatgt gcttgcagga gcccatagag aacagaagag agcttctcaa 75300 gcgatcctcg gaaactatga gctgtctgtg ggtgctgaaa actgaacccc catcttccat 75360 aagagcaata agcattctta actaccagac aatgcctcca gccctttctt tttttttttt 75420 attacgtatt ttcctcaatt acatttccaa tgctatccca aaagtccccc attcccctac 75480 ccacccattt ccactttttg gccctggcgt tcccctgtac tggggcatat aaagtttgcg 75540 tgtccaatgg gcctctcttt ccagtgatgg ccgactagga catcttttga tacatatgca 75600 gctagagtca agaactccgg ggtactggtt agtccataat gttgttgcac ctacagggtt 75660 gcagatctct ttagctcctt ggatactttc tctagctcct ccattggggg ccctgtgatc 75720 catccaatag ctgactgtga gcatccactt atgtgtttgc taggccccgg catagtctca 75780 caagagacag ctataacagg gtcctttccg caaacgcttg ctagtgtatg caatggtgtc 75840 atcgtttgga ggctaattat gggatggatc cctggatatg gcagtctcta gatggccgat 75900 ccttttgtct tagctccaaa ctttgtctct gtaactcctt ccatgggtga ttgtttccaa 75960 ttctaagaag ggacaaagtg tccacacttt ggtctttgtt cttcttcagt ttcatgtgtt 76020 ttgcaaattg tatcttatat ctcaggtata ctaagtttct gggctaatat ccacttatca 76080 gtgagtacat atcatttgag ttcttttgtg attgtgttac ctcactcagg atgatgccct 76140 ccaggtccaa ccatttgcct aggaatttca taaattcatt ccttttaata gctgagtagt 76200 actccattgt gtaaatgtac cacatttttt gtatccattc ctctgttgag ggacatctgg 76260 gttctttcca gcctccagcc ctttcttaat cactggacta tctctccaat ccttccttat 76320 tgacatatgt ttaaaatgta aagtattttt gttatgtgtg tatgcaaatg aatgtgctac 76380 aaaaaggatg tggacaacat tgaagaattg gttctttacc cacccaagga tcaatcccag 76440 gctgtcattg actgggcttg ctggccactt agtaaaaaaa aatattacat ttggtaggaa 76500 ggctggctat cctcggtcta atgaacttcc tgacccaacc atcccagtga tatattatag 76560 acatacatca ccatgtctga cattgatagt tggattattt tttttaattt tgaaataagt 76620 tataacacat taagtttaac aatactataa cgttcacatt ttcttttcca ccagtttccc 76680 ccaagtaagt tagccttaga cataatcaaa accaggagat tgacattatt atattaccaa 76740 gcaaagacgg atgctaggtg gttcacgctg gtttcttgca cacacttttc tgatgtattt 76800 taaacatcag atgtactgat tcctgtaccc accactacca tctggaaata gcactacgaa 76860 atgtccctaa ggacactctt gttcacaatt gtaccttccc tttcaccacc agacatggca 76920 actcttccac acagttgtca cttacagagt cttacatcaa tgaaatctgg ggtgaatgtg 76980 ccatccaaag aaacacagga accagagttg ggttccagga acccatgaga aaaagcttgg 77040 gtagtagcat acactggtag ttccagccct gggaagacag aaacggtgaa ccactgagat 77100 ttgtagcaag ccaacgctac cttttctcac atttagccct acttggtgag cccaggccaa 77160 tgagagaatc tgtctcaaga agcaaggtgc gcgacatgtg aggaagaata ctcaaggtct 77220 cctctggtct ccacatacac acatgtacat acttatgtac acccacacat aaatttgctc 77280 ctccctttgg acacatgtcc aggagtgttt agaaactgga tcacatacca tgtaacctcc 77340 agagactggc ttttttctca ttgatgtgaa tgtagcatag tttaggcatc ctcacgctga 77400 agggcttttg gatcattttt aattgaagct atttacaaat aacactgcaa tgaccactta 77460 agaacagtat cgacatggat ttttatttct ttgacaaata tccaggagca ggatcgctgg 77520 gagtattagt tacttttata acacaccgca acaaaatcag cttttgggag ggtttatatg 77580 gggcttataa aggaatgagt ccagcaccat catggtgggg aagcatgtct ggaggcagga 77640 atggtggcta aacagcagct gagagctcat gtcttgaaca ataaacaaga agcagagagg 77700 ctaaatttga aatggcacaa atcttgaggt cttaaagccc atccctagtg atgtattcct 77760 ccagcaaggt cacacctact aagcctcccc atacacccat cagttaggga ccaagtattt 77820 aactgcccag accatggggg atatctcatt caaaccacta cactggataa aggtaagctt 77880 aatttaaatg agaaatgccc ccgacaggct caggcattgc acacttggtc tccaattgtg 77940 ggcactattt ggggaggttt gggaggtatg gtcggtcctg ccataggaag tatgtcactg 78000 ggggagggct tcgagaatat ataaccccgc tccacttacc atttgctccc tctctactgc 78060 ttgtttgtgg ttggagatgc agtcagtctc ttggttccct gctcctgcca ccatgcctgt 78120 cccttgcaga cggactcttc tccttccaga cccaaaagct aaaaataaac tcttaattcc 78180 ataagttgtt cttgtcatga tattttgtca caacaacagg acaattacac ccctctctcc 78240 ccaaggttat ataaatagta aggacctctg tggagacagg attatttcca gtctgttgtg 78300 ttgtctgcaa atcatgtaga gagctcttgg gatacaccct tcatgagtca tcacctatac 78360 tcagggtttt tgagtaatgc agtattgtac ttgtattgtc cagtgttcct gtcagtaagt 78420 tctcacccat aattctgtaa gtaatccaga taaacacatt ggcttttgaa tggacacttt 78480 ggtaggatag tttctttagt ctgtcattgg tgctttatct gggctgagta gatgtttgca 78540 tgaagtctac actctttgtt ttttgtttgt ttgtttgttt gtttcaagac aaggtttcta 78600 tgtatagccc tggctgttct ggaactcact ttatagacca ggctggcctc gaactcagaa 78660 atccacttgc ctctgcctcc cgagtgttgg gattaaaggt gtacgaacac cacgcctggc 78720 tgaagtctac actcttataa aaaaaatagt cctgttctca cttcatttct gttgctgtga 78780 taaaacacct tggccaaaca gaacttgggg aagggaaggg gttcattatg gcttacagtt 78840 ccaggttctg gacagtcgat gagaaggcaa gcttagtcac ctcacacgca tagtaaagga 78900 cagagaaaaa tgaatggatg cctgctgcct gctgcctgct tgctgactgc ctgcttctgt 78960 ttatcccctg ccataaaata gttgagaacc ttctgcatag agaatgttgc tgcccacact 79020 gggctgggtc ctcctacatc aatcaacaat caagacagtc tcccacagac atactcacag 79080 gatctaggca atttctcaac cgagactctc ttctcaggtg attctgggtt gtgacaagtt 79140 gacagttaag gctaaccatc acagtgtata ttagaactta tttgttctaa taaacttagt 79200 aattcccatg ggtcattagc tctacctggt aggaactatc tttgtacact ttgttaatac 79260 aaattgacta cttgttttaa tacctcatat aaaatgacag ctgtggcatt tgtctttcta 79320 tgatagagtt atttcactta gcatgtcttt aaagtttatc tgtgttctca tgtattacag 79380 aaatattcat aatgttaagg ctgaataatt ttcatgtgca tgtgtacaga cacatgcaca 79440 tactttcttt gctcatttgt tatgagggat catacaggtt gtttccttaa gttggctact 79500 gtgaatagag ctaccatgaa gatggggtga aaatatctgt ttgtgatatt tagaatggct 79560 cttttgggta aaattctttt gaatactaga tcatgtaaga gtccaatttt taattttgaa 79620 aaaatattta tttttatttt gtgtgtatgg gtgctttgct agcatgcatg tataccacac 79680 gtgtacagta cctacctgca gaggtcagaa gagggtgtta gatcccctag aactggtgtt 79740 acaggctgtt acgagctgct ttatggtgct ggcaaccaaa cgtaggtcct ctagaagttc 79800 agacagtgct cttagccact gagccatcat cttctggatg tatttttgag aacctactat 79860 actcttctga catggcatat cattttacat tctgtcagtt gttcaaaaag cttggagttt 79920 ccccatatac tcattaatac tggtttatca ccatcatcat catcatcaac aacaacaaca 79980 acaacaactt tattttataa tggtaattgt gacagatgta aagcaatatt tcatgttggt 80040 tttgatttgc atctttcaga tgtcagtgac tatatcctcg tgggtcactc atatgacttc 80100 actgtagaaa tactttaatt atttaacccc ctattcctcc cctctccact ttttatagta 80160 atgagggttg aacccagggc ctcatacatg ccaggcaaga acattacccc tgagttgtat 80220 cagtcagccc tactcaattt ttcagcctct tttaacatca agatagtaaa tgtgttgctg 80280 tttggaaagt ttggggattt tattacatct tgggagttcc ttatatactt tgataattaa 80340 ccccttagtg gctggatggt ttgtaatatt tttatccttt ttcctgagtt gtctcttttt 80400 tctgttaatg ttttctttcc ttatgtgatg ctatattggt ctgaatgaga agtgttccct 80460 gcaggcctga gcatttgaat gcttggttcc tgtcttagtc agggtttcta ttcctgcaca 80520 aacaccaaga agcaagttgg ggaggaaagg gtttattcag cttacacttc cacactgctg 80580 ttcaccaaag gaagtcagga ctggaattca agcaggtcag gaagcaggag cttatgcaga 80640 ggccatggag gcatgttact tactggcttg cttctcctgg cttgctcagc ctgctctctt 80700 atagaaccca agactaccag cccagagatg gttccaccca caaggggctt ttccgccttg 80760 atcactaatt gagaaaatgc cccacagctg gatctcatgg aggcacttcc ccgattgaag 80820 ctcctttctc tgtgataact ccagcctgtg tcaagttgac acacaaaacc agccagtaca 80880 gttcccagtt ggtggtgttt tggggggagg tttggatacc acaagcttgt tggagaaaat 80940 gtatcactgg gagtaaactc tgagaacaga tagtttccca catgtctagt tcatacctgc 81000 tgctttgtgc ctgtggtccg agacatggct ctcagatctc tgctcctttc accatgcccg 81060 attgctgccg tacctcacac catcatggac acttatccct ctggaaatca tgaggcaaat 81120 aaaatcttct tcctataaat cgccttgtac tcggtatttt atcacagcaa tagaaacgga 81180 actatgtaga tggggtccct gttgtctact tttgcgtctg ttgcctgtgc atttggtgat 81240 gtatctatga aatcgtagcc aagaataaag gcaaagctct ttccccatct gttttcttct 81300 gaagttttat catctggggc atatctttaa gtctctaatt catttagagt ttgcttctgg 81360 gcataactct ttttctcaat atggcagtgt tggggactga acccacagtc ttgtgcatcc 81420 ctggtgagta ctacttcatt taatgtttag agaagccccg actattccct aacctgactc 81480 tccttctcag tcaagttttt attgatggac gcagctccag tttgtatcag agaatgaagg 81540 ttagcacagt atctgttttc atggaacttc tggtaaaata tacatccata ggccaatata 81600 catgaaaaac aactacaccc ctgactgcct tcaccaacct attgctggta ttttatttgt 81660 gatagttgga ggtagggtgt gtgcctgcca ctgcatatgc gtagaggcca aaggacagct 81720 ttgtgagtag ttctctctta cctttcttag cttctaggaa gtgagctaag gtctgagggc 81780 ttgtgtacaa gtattttatt tgctgacgca cctcaccacc cttcattacc aacatttcaa 81840 tgttccccag ctgtatatta aagggcccaa gaagtctata atgcagtaac ctgcttaaca 81900 tcactgacac agaacttatt aaatttgcat gacaatgtaa aacatacgga ttagtatata 81960 atttaattta aagaaatctt aatttgcttg cttttataaa tgcaatttgt taaattgcct 82020 catggaacag ctattttctt cagaggcaaa taaaagagac actaatattg gtaatcctgc 82080 ttcacccagg aagagcactg gtcctagtca ggagtccagc accgccgtca ctgttgggta 82140 tgtctttaca tttaagtcaa gttcagagtt cagagtaaga gactatccca gatgctaggg 82200 tggctttgag ggttctgaga acccaaaggt cacgtgaaag atatggttac tattgtcagg 82260 ctgattctaa tcagaaatca gagtctggag atggagcaga taccattgag aagcagttag 82320 gaggtggtaa tgtggcactg acaacccagc caaggctcag gtagcggtgg acatgcgcat 82380 ttgctttctc ttgtgtttat cgggcaacaa gcctgggtga actggaaagc ggatgcggtc 82440 tgaaggtctg catgaaacca cagatgtcga tccgtggggg gcagactccg ggggcatgtg 82500 tctcaggggt atagcacttg gaggtggcaa ggactaattt caaggtctca cactttaaaa 82560 gcctaaagtc caagcatagg tgggagccca gcctagactc agagcgcaga cgcggacaga 82620 gagcatgcta ggagcctctg ttggacagtc aaacatgacc gagtaagacc gaaactccat 82680 agagacagac tgagggaagg gagaggttaa agacagggaa cctgggctct aagatctccc 82740 tgtaacagct ctccctttgt aagattggat cgaatcccta gggtaagggg cacagctgag 82800 cttccaggtg agacaagagt agggagaaga accatcaggt aagacaatat agttagagcc 82860 ccaaaccaat gaaccaacta accaaccaac caaccaaaca aacaaataac ccaaagacca 82920 aaaaaaaacc cctaaaaatc aaaacccaaa ccaaaaaacc tcaaaacaca aaacaaaaca 82980 aaaagaccca tgagccacag gaggaacaca gttctgtgaa ccctgggctc aaacaagggt 83040 atggatagaa attaaatgtt atatggatgg aaggggacct ggagaaatcc cagtgttttc 83100 tcttggatga agactcttga tccctgaaac taagtctcac aagcccagat gaagttgtga 83160 gtttaaatct tgtcaagtgt gaaactagag ccaacagtca aatgcctccc tccaccagct 83220 aaaccagtcc attttaacct gtggattaaa ttgtggttta attttaggcc agaaaaaaat 83280 ttttacttgc cataaattta agtgctttag atacccatga cataaagact ggagcattat 83340 gtaaaaggga ggcacattag aggtcattta atgacttgtg ccacttcaga gctttctatc 83400 acatagtcca cagcaattca aagtcatgac ccctagaact aaaaacatta cccatcagcc 83460 tttgaactct gaaaccggcc aagtgtaata agcccagagc aaagtacaga ctttccagtg 83520 actgagcaca ggcagtgact agaccagatc ggccatagtg agaccacaca ctggccctac 83580 tcagcgggca ttcactcagc ctagattcca ttttggttgc tgctcaccta gtctcaaaaa 83640 gtctcagcta agtgatgtga cagccctttg tgctaggaca tgatctgttc taaatatctt 83700 tccacaggtg tgctgcaaat caccgtcaac ctgataagaa caatccctac aataaagtga 83760 gttatttata ccaacatcct cccatgttgt tgaaccaatg aagcaaatgt ggaagaataa 83820 tgtttggctc ctttctgttt tgaaaacatt ttctgggaaa gcaggtgttt gcagactaaa 83880 tagatatgca tgccatgggt gatagatagt cttagatgtc aggtcagttc ttcatcgggg 83940 acagatcttt ggaggacact gtaatattgg tcatgacaga atagattctg atagaagttg 84000 gtgtctaaaa tgaaaatggc ttggagacca caggtgatcg gggagttcct catggctgtt 84060 acttatagga agaattcttc cctcctataa gccctgctcc atccagctcc caaagatcaa 84120 ccagtattaa gactgacgtt gtttaaagca ccactatctg tccctgtatc tactccaacg 84180 cattgagcca gctatatagc actttttttc cgttccccaa taaccctaaa tttcttctac 84240 ttcttgttgg agatgtttcc tttgccccag atgtctttca tccatccact aagtacttcc 84300 atcccttcct gtactagcta attctttcct cccctccaag actcatcaca ccaccgtgcc 84360 ctccaggatg gcttctctca gtcctcactc atggactgag aggtgctccc acctcacttg 84420 cctggtaggt gctcccacct cacctctgca tgccaggagc taagaaaagg aagttagaac 84480 attactagag attcgcttgg aaggaaggca ggaactgaga tggaaagatg gggggggggg 84540 gaatgataaa ccatatggag ccacatgaaa cctggggttg tgtgcagtaa ggggtgaaaa 84600 gggatctggc taactgggtg ttctcaacgc aaggaaagtt cataagacaa acccaaatga 84660 gaccattaat actgccaggt acagggcaat gtctacaaca acgacgatac agaataaccc 84720 gtggcctgga gaggtggctg ggtacataga gtgtttgcta agcaaacagg aggactggaa 84780 accagaactt taggatcata tacattccag atgagcctgg cagcccacca ataataccaa 84840 tactaattag gcagaggcaa ggggagctcc agggcaaggt ggcttgctga actagcaaaa 84900 tcagcgtgct ccagattcag ctggaaaccc tgtttcagtg aataagatgg aggcaataag 84960 acatccagtg ccaatctctc tttcccacat ccacatttgc ttggtcacac aggagtatcc 85020 acatccatcc acatgcatgc atacacagcc ccacacgcat acacatgtgg gagaaaagga 85080 aaaaggaaag aataaagtag gtagacatga cgtcgagaag caatttaagc agggtggatg 85140 tgttttctgt ccatcccttg tgtgtttgtt tctctttcct aagctccact gttcattagg 85200 ggaaagttca actcacataa acttttcatg tcttcttcag atatcctcac cttgagatga 85260 tgtagtgagt agggtcttgt gcacaaatcc ctcagggttt ggagtggggt atgggtgtca 85320 aagggcacag gtgaaacaaa cctggctgtg cactgattct tgtagggtct agggttttat 85380 ggggtgcttc tacttttggg gggacttgac aattattcac aacaaaacta caaaacagcg 85440 catcaggaac tatgggttct tctcaagtgg agggcattac atattaactg aatgcgtaca 85500 catgccttta agatgggata tgcatatgca ctgtctaaga taccccgaga aggcactggg 85560 tatttcagat gctgaacaca gattttccta agctcttgcc ttcaggccaa attctgcttt 85620 tcctggacta tggcactgtg cagagaaaaa ttatttgagg gcaaggtggt gagaagtctt 85680 aaaggcccca tagccgagga tggggtaggg ctgtctcttc tcctgtgatc actgaaggcc 85740 tcttatattc aacagacacc aaatctgaga ggtcctacca tggccaaggc catatcccct 85800 ttcttatgtt cagagatgga aaaggaggat aatcaaagtg cttaactcac agggtcatta 85860 gtaccatgaa tgtgttaagg tttagtccag tgagttgtta gcatcgggca tctgtgcttt 85920 actatcagag ttagtgtttg cgttggagtt agtgttatgg gtcaatagtt accagttagt 85980 cagacacaca ctatgatgga taggttgttt atagacagag agaagacatg gttttctgaa 86040 tataagacgg ggaaggagta ccagggctag gaatgagcag agaaactatt tgaggatgca 86100 gctgtgtata atttaggcag ggtgtgtagg ggaggggaga gatgtgaacc aggaagcaag 86160 gcagggctgg atggatgatg gtgggcctgg catggggcag caaagttgag acttgaagca 86220 ggaggccccg aggagccagg gagtgacctg gtccagggag gggcgagatg aaaacatact 86280 tcaggaggat taggctggca tgagatataa agtgtctcgg ttttgggcag gttcagacca 86340 agggtcgaga agtccctaca gagctggaaa tttctagact tcctccagcc tcaggctcca 86400 agctacattt ttgccaggct gagtgggaat cctggagttt tccatgggac ccaacaattc 86460 atctttgtgt cttctgttac cctcagcttt gtttttgtgt tgcgtaacat ttccaggtct 86520 caatatccca agcaagctgg agttttcaga ggttaaactc tagaacctac ccagcctggg 86580 tggcatctct ggaagcaaaa agagacaccc cccttttgac cagagatcct tgtagacaaa 86640 tggccttagc tgaccctggc tgttctttgg ttcatataac aatactcctc tcctctctct 86700 agtccttctc ccaacctgtt tagacacaag acttacataa accttctcca ctcccctctg 86760 cttcttcagg tggaaccttg aagaaaaaaa aagttgttac taggccaagg agcaaggttt 86820 ggaaagacac gatgtgctgc caaatccagg aaagcaagtt aatgagaagg tgagactctg 86880 tccacccact ttctttcatt ggcggaattt ttaggtaaca aacatagctc caggcaggtg 86940 tctgtgcccc actcccatgc tgagcaggag ctggacccga gctgcaggtc ccactcagcc 87000 attcagtggc ttcatctctg caccatctcc agacccctat gtccaattgc acagcagtga 87060 tccaggtcct tccctctcga agtccttttg agctctagct ttccaggatg cccttctacg 87120 ggtcagtgtg ttggtctgga tgagtcctgg gtctgaccag aactactctg aaagggaaga 87180 taaagaaaga gccttagaag aactagctgt cctaacaaca ggagaccccg tactcacata 87240 ctaatgtgca ctcatgagct catgtggaag tatacatata agcaacactg gtgagcacat 87300 gcacacacac acacacacac acacacacac acacacacac acacacacag catgcatgca 87360 tgcacgtaaa ataaagctta taaaacaaaa caaaacaaca ccaagaagca agcaagcaag 87420 caagacctag ggtttttata catctgttgt gggattgttg ttgttggtct tattgtgttt 87480 tgcatttttg gtttgagaca ggttctcact atggaaccct ggctgacctg gggactgcct 87540 gcctatgcct cccagagtgc tgggatcaaa ggtgtgtgcc acccatgccc agctgctgtg 87600 gattttatag gtcgttgatg gctgggtttt ggttatgagt ttaacactgc tgttgaatgc 87660 agagagcctt ttaaatcgag catcaatgag gggttgtttt gttactgagg ggctggacat 87720 gggtttgctt aggactgaga tttgctttct gttgcctccc ttgttctgag cgcaattgct 87780 tagctagatg tcgatggcct ttcaccatgg gtttcctgtg agcgttacaa gctgagattg 87840 gcaggtgcat tgaggaggtg ggcaagggga ggggcgacag cacaatgctg tttccctatt 87900 ggtgctaagt gctgttcctg tttaataagt gcagttttga actggaagag aggaggtgtg 87960 aaaacaccac acgagtcccc taaaatagcc tcttaacaac caaatcagga gtagaatctg 88020 gtctccgttc aggaaacagg gtgcttggat gtccactccc ctggggctcc ttagcccgtc 88080 tttcccctta ggttcaaagg ttcttcataa cctttgggtc cattgacttt acttctgata 88140 atatcaatga cctggggaca tccaccttac atatgaagga cccctgtcgg catgagtagt 88200 tgtctattaa ctgtcaccat gtagtcagct tctctggcag agatgattac aatctaagtc 88260 tagaatgctg ggacccttta atacagtttc tcatattgtg gtgaccccta atcttttttt 88320 tttttttccg agacagggtt tctctgtata gccctggctg tcctggaact cactttgtag 88380 accaggctgg cctcgaactc agaaatctgc ctgcctctgc ctctttgttg ttacttcata 88440 cctgtaattg tgctactgtt atgaatcata atgtaaatat gtgtgttttt gatagtctta 88500 gcccctatga aagggttcct tgaacccccc aaaggggtcg tgccccacag gttgagaacc 88560 agttctctaa agagccattt atgaccccct ctttggctat caggcttcta aaagaatttc 88620 cctaggaatc tccacatggg agaagaaata gccaaaaccc cagggacatc cagaggaaga 88680 cctaatgttt tgtgaagaat tttgcaactt attagatgac ttcatagaga gttggcgctg 88740 agacggccgt gggcagaggc cacaccttgt ctgaactatt caactatgca tatcttttct 88800 ttttttatgt accctcacgt atggaatttg aaatcaaact tggatgtagt ggtcactgta 88860 tttcctttgt ccctctttcc attgtggcca ctttgaataa atctccattt taatttggct 88920 cttttatttg gcttgttcag gataggtggc caaacctggc tagttggggg ctcaggttga 88980 aaccctaatc tggtaatagt aacatatgtc ctgagtctgg acctgtacag ccatgctcag 89040 taatgaagaa caagcagcat attggatctc tcctaagggg tgaatttcac ccctgctaaa 89100 taagcaataa ttgatcaaac aaggactggc tgcatgcttt ttatgctcct cagtattcgg 89160 gggcagcctc ctctttttca ctactccaaa ttctcattct gacttgagct tagagttgtg 89220 tttactccag actcacgcgg tttcccttga ttattattgt gtgcctctcc ctctgaagct 89280 gaagaaatag ctgatcctta cgtgcttgtg gagtaagcaa agaagtcaca tgactggatc 89340 agtacaaaaa tgtattccgc tttgctgtag tccagtgaat ttgaagccat ggatattttc 89400 ccttttaaat gtacagttca tgtttagaga aaggaacgca acgatgtatc aattgcttgt 89460 atacttactg tcaaagtagc agaagttaac atttaggctc agtacttttg aatttccttc 89520 tttcttgaag aaatggagca ttacattcat ttccatctct actgagaaaa ccagggctga 89580 gtggccagcc atgtgcttgc tgtctaaaat gcactgatgg ggactggaga gatggctcca 89640 tggtaaagtg tttgttctgc aagtatgaga tcttctgtct agacatccag aacaacatct 89700 aaaaatatct agatgtgatg gtgccttcct gtaaccccag caggggtagg aagggaacaa 89760 gtggatcctt ggggctgtct gatcagccat ttcaaagaat cagtgagatc catcatgaat 89820 gagagaacct gcctcaggaa gtaaagggag aggctggagc aatagctcat ggttaaaagc 89880 actggctggt tccagctcta gcagcaatct acacggtggt tcacaagcct ctgtaaccct 89940 agacctagga tgtaatgtgc ccttccagtc tccaaggata ctgcatgcat gcatggtgca 90000 cagacacaca tgcaagcaaa tacccaggca caaattaata ataatgatga tgatgatgat 90060 gatgtagata gatagatgat agatgataga tatgtgataa atagataata tagatgacag 90120 agataaatag ataataggtg atagatagat acctagatgg atagatagag ataatatagt 90180 tagatagata tagctagata tagtatagat agataaatag atagagatag ataaatagat 90240 agagatagat aaatagatat agtattgttc aatagagata gataatagat agatagatag 90300 atagatagat agatagatag aatagtatag tatagtatag tatagtatag tatagtatag 90360 tatagtatag tatagctagc tagctagata tgagtgtggt agcacatctg ggaggcagaa 90420 aaggtctgca agtttgaagg cagcctggat tacaaagcaa attccaaggc tacatagtga 90480 gacattatct tttaaaagta catatataaa gtaaagcaga agcagaaata aggaaggcac 90540 tgcccgttgg cttttgtcca catacacgta cacactctta ctcacatgtg cacacatata 90600 accagggcca tacagaatac acacacacaa agtctaagaa tctagggcca acgagatgtc 90660 tcactgggta agggcacttg ctgctgagct tgatgacctg aggttaagcc ttgagaccag 90720 ctctcacaga ttttcctctc atctctacgt gcatgcaagt gtgtgtgcgc gcacgcagga 90780 gtgtgcatga gaccagtaga ggggtctttt tgtccattgt catcactgtc ttcaaatcag 90840 ccatatcagg cacttggaac ttgtcccttt catatacttc cttagaaatc cctccgttca 90900 atgtcacttt tctcatttgg actggttaaa caattccctg gactgttctc tagaaagaca 90960 gatgtgtgaa taattaactc aacctcatta atcagttgct accccggttt ctaagcttca 91020 aagctgagtt acttgccctt agagcataga attggtttat aaatggtgta tgcattcatg 91080 tatgctaaag gaaattatac gttttataga actgagaata tgtaaagtat ataaaatgta 91140 ctgtgaaaaa aattaagaag atagagacga ctttttaagc aaattcaaag agcttaaagt 91200 cttattgtag atagtgtttt aaattttttt cacttataat tattgtgttt gtgtgtgact 91260 gtgtgtgtgt gcatgtgtgt atctgttcat gtgcatgttc cacagtgtgc atatggaggt 91320 cagagggcaa actttcagag ttcacgttcc actgtgtact cagaggctca aattcaggta 91380 gtcaggcgtg cacagcaagc atctttctac accgagggat cttgcaagcc caagtgacat 91440 catatcgata ctcttaaaag tcattgcctt caagggcaaa ggtgatgtca taaaaaagct 91500 cgtgcttaag tcagcttaag tcagagagca gcctgcagag ttctgtgctg ctgacaccgt 91560 gctcattgca ctaagcctgg aactatggga agctcacaga taaccaatac acagacagtt 91620 ccctgaagac tgtagtcaag gagacgggtg cacacagcta aacagaccac aagccagcga 91680 agcagatgct gtgacagtca cttaaaggac atgctgtggg tgagactagg gagctgggag 91740 taggtgccaa tacagcaaaa ttccttgtgc ttaccaaatg gtccatcctc actgtcagat 91800 ggcaggggca tgcaggacac agtggtggtc tttgacttgg ttatgaagga tgaaaagact 91860 tcaacaggaa gacaaattgg ggatgaagat gtaggcagtg gagggaaagg agaagcagtc 91920 taactggggc tgaagagata accagaggac acagtgttgg atattttcac tgagattaat 91980 caacagttca gtctttggtc catcagcccc tcctccacac acacatcccc cactcgtttt 92040 tttgaggtat ggttcacgtg ttccaggcta gcctcaaact caccaagtgg ttagctgaag 92100 atgaccttga actttagact ctcctgcctg tacttcccca gggctgggat gacaagtgtg 92160 cactgccctg cctcaggtgt gcactgccac gcctggcttg tgctgttact agagatggaa 92220 cccaaggctt ctggtgttct gggcaagcac agcctggctc acatccccag ccctggtcca 92280 ctcttgtctc tttccacagt gatctatcta gtcttggtcc cagcaccttg ctcagaggga 92340 gtggtcaccg atgacctctc cgctgtttac ctggatggac cctccttagt ccctcattcc 92400 ttggcctctt gctgatgtca cttgtcacgt caaccttgat gtcgatggcc acactgacat 92460 ttgggtccct tgtggtcttc atctgtaaca tcctgtccac cgctgtattc tctttgtttt 92520 gttttgtttt gttttgtatt ttcgagacag ggtttctctg tgtagccctg cctgtcctgg 92580 aactcactct gtagaccagg ctggcctcga actcagaaat ccgcctgcct ctgcctccca 92640 agtgctggga ttaaagacgt gcgccaccac tcacggcaat attctctttt atctatgttc 92700 agatgccttt tctctgtcag ttcttcctca tcttacatct ttatgagcat gtctcctgca 92760 gtcttaagga catgccctct cctacaccat gcctgaattg ttcatatcta actttcaggt 92820 ctcatcttaa ttattactca ccttaggaac agtttcccca aactttgcat ctgtccagta 92880 atgagcctag agataaaact ttatcagaga taaatgcctg agtacccagc tgtcttccta 92940 gattagccca tgccattctt tagtttcttt ttttgcataa cccgattata aatgtaaaag 93000 tcatgtttac cataatattc ccagcactta acacaggcta atcaatatca agttcttaat 93060 aaatattcaa tgagtaaata tgttattcag gcaacatcaa gaatgctggg ttgaataagt 93120 agggttatat aggaagaaac aagagaagaa ataggctgga tgaagaactt tagctttcca 93180 cttcaaacat ttaaggctat tctacagata aaactgctat ttaaagttgt ggtccagtct 93240 tcctctagta acatgctttg ttagctggtg tagtgtaacc aattagccag tttagtcaag 93300 ggttagcagg gtagactaca ccacaactgt aagagcagca agacagaagg actgaacagg 93360 gatggtgccc aagagaatgc tgggagttga tagcgaggca aggccccatt cggcatcctt 93420 ttctgccctg ttatcattca ctaggagaaa agagtgccta aggaaagcca gacttagaag 93480 gaagagaggc agagagctca tttcctgatg cagaatcagc cggcaggata ttagtctata 93540 gctcaggggc tccctgagca ggagctgtgg atttgagaga tatttgggaa gaactattag 93600 atgaagatgt gagggttgtc aaggaaagca gacaaaggga agcgagtcaa gagctaggca 93660 tgtgtatgaa ctcaaatagc aacaacaaca acaacagcaa caaatgccat gtatacatgg 93720 aagagctcgt ggccgaaatg acaaaggaaa caaatgtttt aaactaaatt aaagaaatga 93780 tcacgtttag atattgtgct taaaatcttc aggaggtgaa aggtgatctg gaagctgaca 93840 ccgagcaggg gcaaaaagag agcatgtcac agttaggttt agtgggtaga aggtaacatg 93900 gggatgatca gagaaccact gaagtggggg aggggtggtg atatctttgt tcttcagaca 93960 cagaagagca gaaacaagga ctcaggcatg cgtcgtgaca gggttagagg cttggttagg 94020 tagaaagtta ggtttccatg tgtccgggga gcggggagga gaagaatgtg ttcagggcaa 94080 agatccaatg tataaacttt tatggccaac agaatgaggg ctctcagcta cagaggaaac 94140 acttaatccg cctgtaaatt tcagtccgtc atactaacct gacatggcag ctggcgtcat 94200 cgggacatga gcaatggttc caagcagagt tgattgtgct tcctcactct aattattaac 94260 cagtcactgt gttagctcat tcacgttgag aagcctcccg cctcactacc catctgtatg 94320 gagacttctt ttctacgaaa gaagtatgct cattccaaat aacatcaagt gtccaaatca 94380 ttagttttta ctctttgttt tcatatgcta tgggttaagt gcagagctat taaaatacaa 94440 aaaaaaaaaa aaaaaaaatc tggaatgagc cctgacaact gacttgattc caatcttatc 94500 tgccattttc tggctacatc attgggagac ttacttaacc tctcttaggt caaactacat 94560 tattggatgt ttcagcccat tctaattttt tttcttttct cagccttttc aatgtgtatt 94620 tactacttga acgtttttca tttaaaaaac aaacaaaaaa aattgaaaaa actcccagaa 94680 catctgctat gtgtcagcta ctgaaggaac acaatgttta agacctaggt tcctctcatg 94740 agtcctaagc ttgctttagt aggcagtgac agaaggacaa actggtccat ccatctagat 94800 aaacttcgga aagtgaccgt gtccatggga agtcctcctt agacactgtg acccttgagt 94860 tcagcctcag agaatggatc caagttaatt gtaggtgtgg aaggttgtgt tttgacacag 94920 gggacaggca agtgacaaga aggcactgag ttcaagggca cagtatattc taagtgtcag 94980 ttgccagtgg ctgaacatag aggagggaaa gaggttagag aagccaagtt caaagctcag 95040 actatgcata cagtataagg ttaaggcact gatcctatag ctatgttacc tatgaatgag 95100 tggtcgttct tgggcaaggg acataactca ataggttttt cgcttttcca gctgtaaagt 95160 ggaattgaaa atggtatggc atagcgacct cacatagaag tattcaaaac aatgtctagg 95220 gcacggttat ggttatggaa gcctcttgaa gggctccagc aagtagagat ttgagttgca 95280 ggcagaggca agcgaagagt ggattggaga gccgtggaaa cgggagtgga aagatgttag 95340 aaaaaaaata cagcctgcac tcaggagagg gtatggagat acaggtgaag cagacggctg 95400 gtgtcagtct tgacagccca tcaggtgaag ataggatgag cggtaactaa tacccagagt 95460 tctacttcga acacatagtt ttctgtttgt accctaacta catgcacgcg cgcgcacgca 95520 tgcgcgcacg cgcacacaca cacacacaca cacacacaca cacagataca tacacagaca 95580 gacagacaca cataaacaga cacacacaca cacacacaca cagagagaga gagagagaga 95640 gagagagaga gaaatgacta tatcataggc tttggggcag ggacaggtgg gcctaggtac 95700 agagtcctaa cttttatctt gctttctctc tttctttctt tctttctttc ttcctttctt 95760 tctctctctc tctctctctc tctcccttcc ttccttcctt ccttcctcct tcctcccttc 95820 ctttcttctt tctttctttc ttctttttct tttttctttt ttcttttttt ttcttttctt 95880 tttgtttttt cgtttttttg gtttttttga gacagggttt ctctgtgtag ccctggctgt 95940 cctggaactc actctgtaga ccaggctggc cttaaactca gaaatccgcc tgcctctgcc 96000 tcccaagtgt tgggattaaa gacgtgcgcc accactgcct ggtctacttt tattgatttt 96060 ttgatatttt ccttccctca caaagaagtt gacaaacagc ctggcagagc tgtgagaact 96120 gaatggagac tgagtaggaa ccgtgccctg gatggtgctt ggctctcata cctcagatcc 96180 tcagggaaat gggctggact ggggcctaga gcactgaatc ccctggaagg cccctgttga 96240 caaaatctct ccccaaaccc gacaaaagca tcacaaactg tgttcttccc agcacctaca 96300 ggagctacac cttagaacac ggatgtttcc caggtgttct tttactgtcc tatttctagg 96360 aacccgggac tcgcacttgt ttgaactcta gggcgtttaa gaagtcactg ataaggcaga 96420 gccagatgga ggtgggatga gcaagaactg ctactctgaa gagagggaca ggtgcacaga 96480 agactaatgg ctcagtagac agaaaaccca cctctatcta cagataagcc aggtgacaac 96540 attagcctcc tcggcagctg tcccctcttc tgctgagacc tatcgattga catcctaaat 96600 gtcactgtga aatgttcaag ggatactccc cacttagcgt agctgctgag ccagggaagg 96660 caactgaatg tcttatttga atgggttgcc tggtgctctc cagaaaggtt ccaggctggg 96720 acggaacctt cccaagtgaa gaaactcact tcacaagcct ccagtccccg ccctgcaaca 96780 agcctgtttg ttaacttgaa cttgcggtga cacacaccga gttcatttcc agtttgcgct 96840 ccagggcaag tccttgacta cacaaagagt gagacagttg gggaatttgt ggactgtcca 96900 gttaatcaga taaaacccga gagagtcaga aaggcgactt cttggaagat gtcaaagttt 96960 tactgaaaac agcggaagtg ttcgcagcta attggtgctt tccttccaca caccccgcac 97020 tccctaccgc cccctccctc cccgcctccc atccctgtcc agtccccacc ccctgaagtt 97080 tgtctttggt aaaccccagc tacttctctc acttgtctct gaggcacaat gggagtcttc 97140 atctgaattc tagggaagaa aagatatgac tccttctagc acgagtgagc tggattgtaa 97200 cagggaacga attgattgat ttttcccccc cttcatctca atgaacacac tatgtagcca 97260 aaccaaggct gatcaacaac actcaataaa aaaggagatt caaactgagg cccagctgag 97320 ccctactaga cctccacaat gcacgctgtc atctgcttct tacatgtcat cacatttaat 97380 tatattaatc ttcgccataa gtaatggagg ggattattat gagccttggt ctagcaactg 97440 ggttcaaggt cacttagcta atatgtaact ctaaatgtta caagtttcta agccaaggat 97500 atgtcctttt ctcaggccga tgctgtcctg ctggccttct aaaatgttag ttctctgcct 97560 cttgttctgt gcctacctca gtgccccacc cccactcctg ctcccccccc ccccattatt 97620 ctgatcacat ttacattccc ggtctcccat tctcctgatc ctgtgtgctt acatcagcta 97680 gaaattatat gcaggacttt taaagagttt ttttcatagt attgcctttt tgcatatata 97740 tatagtgttc tttgtttgtt tgtttgtttg tttgttttca aaacagggtt tctctgtata 97800 atagctttga ctgtcctgga acttactaca gaccagtctg gccttgaact cacagacatc 97860 ctcctgcctc tgcctgacct gtcaagtcct gggattaaag cttgcaccac cacacccagc 97920 tgcttttata gttttttttt tttttaagtt gtgtggtgtg catgttgttg tgttttataa 97980 aatagctgtg gtataatgtt tggggatggg gaacatttca gcaggcctct gccaaggcat 98040 accccttgag gaatcagtca taccacaggc ttagtataaa agggataggg gacctggaaa 98100 aggggcagag gcagagaagg tgggggacag aggaggacag aaagagagag aggccagctg 98160 ggaacatgtt ggggagagat agagagaggg aggaagggaa ggagaaaaga gaagagagag 98220 agggggaggg agagggagga ggagatgaag aggaagggag ggaggagaga gagagagaga 98280 gagagagaga gagagagaga gagagagaga gagagaggga gggagggcaa gcagtccttt 98340 ttatacacca ggttggctca cacttggcaa gcctcaagct gttgctagga agctgttgga 98400 tggagcctaa aggaactgcc aatagtgtgt gtacagatga gtttgtgtgt gcgagtctat 98460 gtgtaagcat gcagaggcca aagatatcgt tctatatgac agggcctctc actgagcttg 98520 aagctagtca ttttccccag gctggctggc cagcaagctt ttggaatctg cctgtctctg 98580 tcctccaaag atgtggttac agggatacat ggtagtgcct ggcaatgcct ggctttttat 98640 gaggatttga agtcaggtcc ccctgcttgc atagtaaatg ctcttaccca ctgagccatc 98700 acctcagact catcttatta attgtttcag tgtcgctgta tcgtggacac aaacatatgc 98760 atgtatataa tagggggaga gcagattatt ctggcatttc gagatcctag cccagtgcag 98820 gtgggtggca gtattagcaa aatcactctg atctgatatc tgtttattca tgtattctta 98880 tctaagtgtt ggaagaagaa atggcacgag aaagcacagt aacttagttc acagtcacaa 98940 agcttggcaa ctcctagctg ggtggtgaca cgggctgtac ccctatcatt caggaggctc 99000 aagcagaagg ctcccgagat gggagctagc ctgggcgttg gagtaaatcc cagggtaact 99060 ttggcttgtg tagtgagttc ttgttttgag agactcacgt ctggggagcc agagcagtag 99120 ctcagtggtt aaatgtactt tctgtgaaat cataaagatg ggagttcaaa tcccagaacc 99180 tgtgtactga catactgggg aaaacctata aggccagctc tggagggagg tggaggcagg 99240 aggattgctg gagttagttg gtgtcttagt cagggtttct attcctgcac aaacatcatg 99300 accaagaagc aagttaggga ggaaagggtt tattcggctt acacttccat actgctgttc 99360 atcaccaagg aagtcaggac tggaactcaa gcaggtcagg aagcaggagc tgatgcagag 99420 gccatggagg aatgttcttt actggcttgc ctcacctggc ttgctcagcc tgctttctta 99480 tagaaccaag actaccagcc ctgagatggt cccacccaca aggggccttt cccccttgat 99540 cactaattga gaaaatgcct tacagttgga tctcatggag gcatttcctc aactgaagct 99600 cctttctctg tgataactcc agctgtgtca agttgacaca aaactagcca gtacagttgg 99660 cttccagcgc agacaggaaa atttgaatct tgattcagaa tgagatccgt cctcaaatga 99720 aaagccagaa agtaacagag gaggacactc aatgccctca gtgggcacgc gcacaggttt 99780 gtgtatgtgc acagaagatg gtatactatt accatacttg tttgtttgtt tgtttgtttg 99840 tttgtttgag acatggcttc tctgtatagc tctgggtatg ctggaccagg ctgacctcaa 99900 acttacagag atctgcctgc ctctgcttcc agagtgttgg ggttaaagga gtatgccacc 99960 accccctgtg gacacagatg tattcacaaa ttttcaaatt gcatctgttt gtttggctag 100020 tgcacctggc acagagcgtc ctccacatgg catggatgaa gacttattcc aattggttat 100080 tgatcaaact aattcatgaa aaagacagct gtctttcacc tgcccagaca aggagcaaaa 100140 attcatcctt atcttatgga ttgtgcacct tgcacaatcc atacaaatat tttttatagg 100200 aataaagatt tggttgtaag attcatatat tacagaatga aacagggaga tacagctctg 100260 acaagattca tccttgggga tgctgagatg acccgctgtc ccagctcgct gccacatcct 100320 gcctcacctg atgctgtttc cagagacctg cttccagagc agcgtcaggg gtggctgctg 100380 attcccatga catcagttca tccagccttt cagacagatc caccaagact tcagttaagc 100440 cctgaacatt ctaagcatac agggactgga taacaaatgc gaaaactagc tttcttaact 100500 ctaaccaagt tttcttattt ccctacacct cccaccccct tgaaaaaaat tcatcacccc 100560 aattcagcag gaagtagtta tggagagttg tcatcttgat tccttgggtt taggtgtctg 100620 gctatggtta ttttaacatt attatagata ttttaattat ttaattatta ttaaattata 100680 gatattttaa catattaagg gatactttgg aaatgatcat aattttagac agggaggaaa 100740 ctagagaggt tagacttgga gattctgtct tttttttttt ctttcctttg ctctagcctt 100800 actttcttag gtaaaaggaa gggagctgaa atgaaaatgg ggactagagt aatataggaa 100860 attagagatg tgagatggat tattcaattt actcttaaac aaaacatttt atagttataa 100920 tctttcattg gtagaaatct ttacattttg atgcagaact aaagttatat tttcttacat 100980 tggtacagaa tttatatatc gatacaggtt taaggttttc actggtataa atctttgtat 101040 attgatataa aatttaaaat taattctgtt actcttagac aggcattgtg cctatacaac 101100 tcatttatac aatacaaggc ttagacccag tccttctaat aggtactgtc acaaatggtt 101160 taagatgatt aagtgatgca agttaatggt cagatagtaa actcatgctc atattagatt 101220 ctgatttaat tacaataaag tgttttcaag attatcacgt aggaaataac taagagtaat 101280 caatgtttaa caattcaatt atattacata tagatagatg gtcttcaaaa acatcagagg 101340 tccacaaaat atggcattta aaatcatttc actattaata ggtcttttga ctaggagaca 101400 tgtctgctcc taacagtacc cccatctcac tgcaaagaag gtactgagta tcaatacctc 101460 ccaattggag ttgctttgat tgcgatgagc tagctgctgg taagaaatag cctaatttaa 101520 ttctacagac aattactgtc cagaaaggac aaatggatgc aggacagttg actgctaagc 101580 tctgccaaaa cagagtaagc tagtccttta tatttcctgc ttcatttatg gtctatcaga 101640 tcgttctgga ccagaagtct gaagacaggt gctccaacat tataaggaat attgaggact 101700 gtccaggcag tcagttgttc ctacaaattg tattaagttt tagaagctat gtctttgtgc 101760 tttctacata ttctgataat tttaaattcc ttctcagatc tctgatggaa ttgaaaaata 101820 gttatagtct cataattgaa cttaagttat ttagcactaa agaagatgtt ctagatttga 101880 aaggatggtt ttctgagggt attgcaagtt aaaatcaagc ataattgagg tacagatttg 101940 taaattctgt aagttaagat agataataga gtactttatc taaattgtca gatatgatgt 102000 actggatgtt ctaagtgtat atcatacctt acaatttaca tacttgttat aactgtgttc 102060 aatgtatatt tgggagaaaa gagtttttaa ttggacaaaa ggggggaaat gtagctgtct 102120 aatgctaatt atgtttcctc ataactattt gcatgagagg gtctgcacat agcttctggg 102180 aaccttccca cagttaattc tgattggtaa ataaagttcc cagcagccaa tctctgggga 102240 gaagaaaaag aggtgggtgg ggcttaggtt tcccaggtgt gggacctctg ggaagagaga 102300 aggaagagag actcaatgcc tgaaaggtag tataggacaa agaggcagag ccaccctgta 102360 aaggggccag gagagagtgg cccagatggc tgctcaattg ggccaggaga ggccaagatg 102420 gaacatagaa attggtaagt agtaacttgg aattattggc gggaggtaga gtctagcagt 102480 atggaggcta ggcagatgcc cagctattgt gctgcttaag tcatattaaa atataaaggc 102540 tgtgtgtgag tttttcatta gagatcataa accattgagg taggaagtaa tgctgtgctg 102600 ggatttatta aaacattaat actacacttg tggcaatcac aggacagaca acttgtagga 102660 atggattttc tccttccacc atgtgggtct agggaattga atttaggtag tttacagcct 102720 tggtggcaag catgttcaag ccagccccaa tttaatgatt ttaattaaag ttgttgtcct 102780 ctgaggcaca aaaaaacccc aaagggttca acacaatcag gattcctgct ggcctctcat 102840 ctacagcttg ggccagaagc cttgctctgt tttgcatttg gaatatttta tctcagtctc 102900 tgtaagtcag gcccagggat gggtatagtc agtacacatg aatttggtct gcataactgg 102960 aaatgtgatt atagcttctc agctgttcca aaggagaagc accatcatat cccattctgc 103020 ctttgtgtga aaaactcctc aaaaatacat attagtttgt atctcatata tatgtataca 103080 tatacacatg tatgtatgta tatatgcatg tatgtatgta tatatgcatg tatgtatgta 103140 tatatgcatg tatatatgta tatatgatgt atatataatg tatatatgat gtatatatat 103200 cacagacaca tataatttag gtcacttttt gtccctatgt tttatttctt tcaaaaccta 103260 ctgctgcgcc ttacacagtg ttttgagacc atcttctttc ttatcttttt tttctcacat 103320 aatatatcct aattacaatc tccactcctt ctagtcctgc tagttcctcc cctcctatcc 103380 agagccacct ttctgtctct aattagaaaa caagcttcta ttatatagat tataataaga 103440 caaaacaaca cctaacacaa ctgaatagga caaacaaaaa ggcagaaaga aaagagaagt 103500 cacaagaaat agatccacct gttcacacac tgaggaattc catataaact ctaagctgga 103560 aaccataata taatgtgcga aggaccttta gggtaaagag agagaataaa actgcataaa 103620 taaaataaag taaaaataat aataaaggaa aatccctgac acagcacagt aagacaggga 103680 agctccaaag actctgttga gttcattctc tgctgaccat ctagttctgg acatgtaacc 103740 tacccttaag agtcgtttgt ttccacagca agactgtctt agaggaaact aaattttcat 103800 ttggaaatgg ttatcaactg gaattgcttc tgggttaggc ctaggggctt gtgtctacat 103860 cttctctcag ctctagggtc caatcttgtc cagacctgtg caggtccatg catgctgcct 103920 cagtctctga gttcatatgt gtatctatcg gcccttgttt tcttggtgtg ccccatccct 103980 tcttgctctt gtattttgcc tccttttccc tgagccccaa aggaagggat ctgatggaga 104040 tagcccattt agggcagagt gctttaaggt ctctcatttc ttacaaatat ctgtctgtga 104100 gtctctgtgt tcccatctgc taagggagga agcttctctg atgatggctg acaagacgcc 104160 aatcaatgag catgtcatca ggggtcattt tatcgttaca tttttgtttg cttaaaaata 104220 gtaatatttg gttctaccct aggttcctga actagcttgt ctcaggttct tggttaccca 104280 agcagtgtca ggtttcatct aatggagagg accttaaatc aaatcagata ttagttggtt 104340 actaccataa gatttgtgac accattgccc tagtgtattt tgcaggcagg gcatcattgt 104400 agatcaaaga gtttgtggct gggttggggt ttacctctct cctttggtag tgtgccttcc 104460 cataccaaga actctggtag ataaaggtga aggctctatg taggcaccag ctcaatttct 104520 ccacgttcaa tgggttgtgt aggtgttgtc ttcagcaatt tttagggtca cttatatata 104580 ctatcatatc atctgcaaat aaacacactt tttcttcttt gtttccaatt tgtatcccat 104640 tgattttctt tagatgtctt attgctctag ctagaacttc aagtgctata ttgaaaaggt 104700 atggagagag tagacaacct tgttttgtta ctgcttttta gtggaattgc tttgggtttc 104760 tttccattta agttgatgtg gtcttgctgt aagctacctt tattatgttg atttatccct 104820 aattattcca ggacttttat tatgaaggag tgttgggttt taacaaatgc cttttctgct 104880 tctaatgaga tgacatgtgg ttttgtcttt cagtctgtta atacgatgga ttacatttac 104940 tgattttcat atccctgcat ctctgagatg gtgcctactt gatcacagtg tattatcttt 105000 ttgatgtgct cttggattca tttgcaagta ttttattgac aatttttgca tctatgttct 105060 gtttgaattt ggtctgtagt tttctttcct tgttgagtct ttatgtggtt tgagtatcag 105120 ggtcactgtg acctcataaa acaaattggg cagtgtccct tctatttcta ctttgtagaa 105180 taatttgagg aatattggca ttaactcttc tttgaaagtc tggtagaatt cttcactaaa 105240 accatctggc cctgggatat tttttttttt tttttttttt tttttggtta ggagactttt 105300 aatgactgct tctatttcac taggggctat aggtctcttt aaattactta gctggtcttg 105360 attcaactat cataagtggt atgtatagag aaatctatgt gagctgaaga gatggctcag 105420 cagttaagag tttgctgttc ctctgtaaga cctgaatttg gttcttagaa cccagattgg 105480 gtaactgata gctgtctgca actgcagttc caagagatat aatactctct cttctagaca 105540 ctggacatgg cacacacaca cacacacaaa gttgaatgca aattaaatgg tagataacgg 105600 tatgtgcact tcagagtttg tggtaagtga cagttgattt acttgatgca acacagagtc 105660 gtctggaaag caaatcttgt gcaaggatcg cctaggttag gctggctatg ggcaaggctg 105720 tggggaattg tattagttgg ttacttgtgg taagacctat cctgattatg ggtggcacca 105780 cctcatagtc tcagtcctgg gctgactgaa aaggagtaag ctgagccctg gcatgtaagt 105840 attaattctt ggctctctgc tcttgactat ggatatgatc tgactatctg tgttaagttc 105900 ctgctgtccc cactgcccag gagtgatggc cctagcctgc aactgtgagc cacagaaatc 105960 ccttttctct taagttgctt ttatttcggg gttgggtggt gggcggtgag accgaggttt 106020 tgtttttgtt tttgtttttg tttttgtttt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 106080 gtgtgtgtgt gtgtgtgtgt ccgtgtgtgt gtcctggctt tcctagaact cattctgtag 106140 accaggctga cctcaaattc agagatgggt tcctttgagg caagcaactt ctgaaggcta 106200 ggaaggctca agcacattgg ctcctgggtc cacagtgctc agcatctgta tttcatgaac 106260 agttcctggg tcctctgcca gggctgtaag aagcacggtg tctgcttaat acaactgcag 106320 ccaaagaaga cagaggtgtt ggtgcaaaaa cacagctctc acatcaaagc aggtaatagt 106380 ttattctgaa gccaaatatg agtcccaatg gcctagtaat acagatttaa gttacccagc 106440 attctgtgtc caatgtgcta agatttggta aagtttttat ggaagcagaa gaagtttatt 106500 acaaatccgg gtacttttca aacacattgg tggaggcatc gggtaggtgg atcacagcaa 106560 agcaggaaca actttacttg aggctcagat gctgacagtg tttcacagcc cattggttgg 106620 ccggaaacta agagttcgca cattctacgg aatttaccta atggcacatg gaagggcaat 106680 aaatggctgt ttaggatcct aaagatgccc caagataatt tggctctaga cttgcaacat 106740 tctaacccct ctacatcatt gacattctaa tcagttccgt cagcctttcc agggctgggg 106800 ggtaggggag gaaggcaagg ctatttgagg aatagcttca ctacttgaac accacacatt 106860 cagggctgca aaggacacac aaggctctgg tctgtggtta ctagacctct catcaattca 106920 tgaggttgta gctatttcga actctggacc atcttgtaga ggaatgtgaa tgaggctgaa 106980 tttccgacat gcctttggac agatgttcgt gatgctaact ctagtttttg taagatagtt 107040 attctcttct gtgaagtgat gaagaatttg acacagtttt aaagaatctc cgtctctctg 107100 actccgtctc tgtctcaatc tctctctctc tctcttcaaa ttagaggttt ggtaatttag 107160 gcacagggct gctatatgac tctttgatag cctcacaggc atgtgtactt agtgtgatgc 107220 tcagcacttt gaaggaagga aggagggagg aagggaggga gggaggagag agaaaggtga 107280 aaggagggat gaaggaggga ggaaagagga aaggaggaag caaggaactc agttataggc 107340 caaagagttt tcaggtttat agacacatag atggttgcag ctgtagcctg gcagtctaca 107400 taaaggacac ataaagacat tctcttggcc acaggttgct aaggctgggt aggaagtctg 107460 tagaaggaag ccagaagtca gtaagtcaca gattaaacat tgagcaaagg accacatcat 107520 tagtcactca atgctagtgt ttatttgtct ttctcttttg tttgtataaa tggaagtact 107580 ctctagggtt tctgtttaat taaaaatctt attaaatata agcaatgaaa ctggcttaac 107640 caggaaactg aacattaaac ccgttcacaa aacgtatgat ctgactagga gaaagagaaa 107700 tgtgctaccc acaccaatcc tggttggaca tgtgcctaag ccgaggtcac tactagattc 107760 agtcagtacc cagagaaggt agcaaacagc ctttgccata taactgaatg aagggccagc 107820 ctcatatctg ggtttctaac tgcagaacac atacatgggt gtgtatccca ggtcaggttg 107880 tgggtggcaa ggccttgctg ctgtataggg gtcagggctc ttcttgcata tctgtgttta 107940 taacaagagt tgcttctggg tggcacagac taggggagac agctcctcct tctagtatct 108000 ttgtcaaggg aacacactat aaccttccat ggtggttagc ttaactattt cctgtgggtg 108060 tgtcccttat taaaactata tcctaaatat ggtcctctct taggaggcat cccatttcct 108120 agtcagtcat catacatgat atacaaagag aggtatcagg gcctgggctc catggctgtc 108180 tagatgtttc tcatctgttg tgggggatgg tgcagcatgc cctgagctgc ctgagtcact 108240 gcaggaatga ggaagactca cagggaggag ggaccaattt ccttcttcag tagagtcaga 108300 gagctgcata ggtgagatct gagctgtgca aggagtgggc agggcttaga tgtagaaaat 108360 ttttaatcct gacctggggc ttctaccctg cctttgactc tttagttccc agatagaaga 108420 cacacacagc ctttataatt acaataaacc ttcaacagca caagagctgg gtggatactt 108480 accctctacg ttattagaat ctactttcct attgataact ctgaattatt acttactact 108540 taactatgtt tcatctgggc tgctcttaac tccaattggc cagccctcag ggccatgttt 108600 ttatgattcc caaaccatgg caccttctcc tttgtcttct gtaccttcta gttctccttg 108660 gttctcctct gacccatgag gtcaccccaa acctctaatc cctccagtaa ttggctgtag 108720 agaattttat ttaaccagta gctttaaatc aagggacaag gtttgcagaa cacaagcttg 108780 taaacaggag aagtcttttg taggccaaga cctttggcta tagaatttag cattacaatg 108840 catagcatca gaccaaacct caacacttag aaactcagca agggacaatg tgggcaaaga 108900 cgtggagtga gagccccctc agggacccca tggtcacctg ttccccatta accacaggtc 108960 tttgggattc tgcaaacccc cagcaacccc cagcagctgc tggaagcacc atcccatact 109020 ccatgagtgt gtatccaagc tttgcaattc tgaagtccta tgagacaatg tgagtagtgt 109080 gtcattgtca ggattcatgt atattgtaga cacaagattt aatgaaatct ttctattgtc 109140 aaaagtgttt ctaaaatgtt tggaattaca ttagagcaga aagagctcca atttatgttt 109200 tgctaggtac cttcccaact tagtagcttt atttatttat ttatttattt atttattatt 109260 tttgatcatg tgcaggctcc caaagtgcat gtggaagatc aaaggacaga tttgtggagt 109320 catttctcat attctacctt atgtgggctc cagagctcat acttaagttg ttaggaatgt 109380 gaggaaagtg ctctacccat tgatctatcc tgctgaccct ttagccattt taaatagcaa 109440 ccatttctga cagcatttgg catctgaaga tcaagaattc aggggctcct tagctgtgtg 109500 ggtctggccc caggctctca aaaggcagtg agcacatctt ggccaaggtt atgtcacttg 109560 ctgttgaaag atcaaattct ttgggttcag actccatctt agacaaactt gacttaagta 109620 aattaacagg tcggtaccta gctccagttt ccagattacc caaaacaaaa cctgagctta 109680 gctccagttt ccaggctacc cctaacaaga tctggatctc cagtttattc tcctctaaca 109740 aatccactgt ctagcaccaa tttccaggtt aatccccagc aaatcccaac acctccaggt 109800 tacaaggccg cccctgccta acaaccacca attaggagga aagccaacgt ttggctccca 109860 gcaccagcca attacaccaa aggtcacagt agcaccccaa ttagatgctt gctaggcttg 109920 aaacactcac ccttcacttg tgatttccta taaaaccttg tcccagtgag tgatcaagga 109980 atttttccat ggaaccgtcc tgtgggtcag cagtgaatca agacccacgc tcgagcttgt 110040 gaataaagtc cctggtgtga tagcatcgga tccggctcct tggtggtctt ttggagttca 110100 tgaacatttc agcatgatac tgtttaactg gcatgggcat tcttcttgtg gtgggcatct 110160 gttcttcaaa tggcgcatct ctgtgtgagt gagtgtctct atggcatggt ggttggtcgc 110220 cactgaaatg acttttgtag aagagcaaac agataaaagg ttcttttgtg ccgtagtctc 110280 tgtcaaacca tcacttctgc tgtattttat cggccaccct gagcatcctt gatttgatgc 110340 agaagaggat tatgcaagca aataaatacc agaggatgtg taccactggg aaacaaggca 110400 aggtcacctc tgaattcata ttctgggaag attagagctt tcagaacagg agctgggcac 110460 atgcctgtaa gctccacact cagaaggccg agcagatggt tcacaccagt ctcagttaca 110520 caagtcagtt aaggtcaacc tgggctactt gacaccctgt ccctacaaca accaaatata 110580 cacgcatgcc agagatgata tagtgaacta gacaccctaa aaaaatttca cctttaacgt 110640 attgaaaaat actgagtaga acattttaaa gatttatttt ggtttttaaa tgttatgtct 110700 gtgtatatgt gtctgagtgt atatcgcatg agtgtatatc gcatgtgtct gagtgtatat 110760 cgcatgcatg aaatacccac agaggacaga caatgtcgcc agaactctaa aactggcgtt 110820 acagacacct gtgatccacc acgtgggtgc tgggagccca atcctgatcc tctgcaggag 110880 caacacaagc tcttagtcac tgagccatct ctctagccct aagagtcaac aaataaacac 110940 acacacacac acacacacac acacacacac acacttaaat ctttaaaagc tgccccatta 111000 tactatcagg agaataacag gtaaaaccaa cacaggagga ggtgtggatt gagagactcg 111060 cggagttgca ggctctcctt ctcctgctct agttcccagt agcttactat tcatttaaca 111120 atgtttatgg ccgtttgcta ggcctattcc tcatctaact cctaacttac ttaacctgtt 111180 taaactattc tatgtctgcc acatggctgg ttacttctca gtatcatata tctaatttcc 111240 tcagagtgca ggtggtgaac ctcccagagt ttcagtctgt ctactggaac tcccagtttc 111300 tacttcctgc ctttggcaaa aaacaccatg cggcatattt ggttttaccc cttgacctag 111360 aaaacctcaa gtgtttattt cctggccctt tattttctaa aatgccaata agataggcca 111420 ccagatcttt aatgacaggt gatgcttcga cacagtacct gagagattat ctctacagac 111480 ataagtgctt aagctggggg ctcctctagg cacatccctc aaccaccaag tgacagggaa 111540 gcttggtgag tttctttcac atggcaacaa acaaggcacc tcagctagca agcaggaaag 111600 caggagataa catgtgacgg atgtaggcgt cagagcaaag acccttccag aaactgcctc 111660 aaatctcaat ggatatttct cagtgatagt tcacatcagg cttttttttt ttttatgagt 111720 actatttttc ttggtttatt caataggctg cttcaattta ccttaatgtc cacttatact 111780 tatatataag cttcttttat acaagctcag tggactcact gagaaagcac aactaggaat 111840 gcaaatcaga cagcaatatt aatacagagt tcaccttctc ctttcaatgg aataaataat 111900 gcgtgcattt gtctctttct aaatatttag gcaccaaaag ttttcatggg actctttctt 111960 ttttatttta tttacttatt aggtattttc ttcatttaca tttcaaatgc tatccccaaa 112020 gtcccccata ccctcccccc tcccactccc ctatctaccc actcccactt cttggccctg 112080 gcgttcccct atattggggc atataaagtt tgcaagacca aggggcctct cttcccaatg 112140 atggccgact aggccatctt ctgctacaaa tgcagctaga gacacgagct ctgggggtac 112200 tggttagttc atattgttgt tctacacatc aggcatttct taattctctt tccacatgca 112260 aggagataaa acctttccta cttctacagt agctctgatg gtcagggaaa gtcaggaatt 112320 ataagcagag tctgggttta tatgggcttg cagccacgct cacccagcat gagttacaga 112380 aacctggctg atagtctgtt tggaaatgtg acccagcatg agaagtgttc tctgtctaga 112440 aacaaacggg agtcctgttg aagcacatga tgtctttggt tttgcccctt cgtttagaaa 112500 gcctaaaaag attattatct ggccctttat ttatttttaa ataaatgtca atgttagttg 112560 cagaaatacc tgcatagaag cccccagtta gcctagagtt gaaatacagg aaagatgcat 112620 ttatcaaact accctgtgca gcagaacaaa ctgaattaga gaccggggca ggaggaagct 112680 tggttagaca cagtcttagg cttggttaaa aaaagggggg gggggcgtta tgcattatgt 112740 acagttaact gggtacctga aatagaccag acttgcttct gagcactgag ggcttaatgg 112800 ttaactataa ggagcctagg gaggaagatt ggttcgagaa catagttact cctcaattgt 112860 gaatggagtt ctgcaaacag agaacattaa gaggaacaga aaaggagaat tcttttggaa 112920 taatagctct ctgccacatg tacccaggtg ctctgatccc caaatacatc aaagctgctt 112980 tgtcacccag tctgtgccag aatgggcttg cacaatgcat ctgagcaccg agtgctaata 113040 tgctctcctg agagcagcag tggggagagt ctgagctggt ggcagtgagc tagctgcaga 113100 ttgctctggg ctggtggcag caggacaagt caattagggg agtgattggg agaaaggaag 113160 gcaagaggca cccagagcct ccaggctctt gtttgaaaaa tggaagacac acctggcttt 113220 ttccacattt ccctaggtgc tcgctagggc ctggtcacct gctctcctga ttgactcagc 113280 agggattggg ttgtaaaatt aggggctggg tgatgaggca gcaacttccg agtgcctatt 113340 ctgtgcatgt ctttgtacat ttatcaaggc cctcaaatca ccctcacaga gcagatattg 113400 gccccttgtt ttgcaaatag ggataccgag gtccagagag aggtactaag actacaaagg 113460 gagatgccac ccccaaatcc ttctttcttt atctccaagt aattctagcc aaggaccaca 113520 tgggagggtg ggtgggctca gcccagctct ccttctaaga acaaaaagga cttatgatgt 113580 cccctgggct ccccatttct ctgtgagagt ttttaggctc aacccatgag gcctagacct 113640 tcctgagaat ggacaggcaa tttatggtag ctgaggaaag gaggaaacat tttctttagc 113700 tgtgtgactg accactgatg agtggcacat gtgcctataa actcactggg taacatacac 113760 agaggggcat gaaaaaaaga ggggggctag ttgagaagaa gggaggggat caggagtggg 113820 agggagagaa gaaaggggag cgatgggtga atatgatcaa tatacattgt atacagggat 113880 ggaaatgtca tgataaggcc attattatat ataattgata aatgctttta caaaatcaga 113940 acagaaagat aagctgagga ggaagttcag aatcacaaat aagtaaaata acaacaggga 114000 aaaatctttc tcagaacgtt gctatggaga tagccacatg ctgtgtgagt ggagacctca 114060 ggttttccta ttgggcaaga tgcattgagt agctacagtc agggaaagcc aggaggtctg 114120 aacctcgatg ccagagagcc catgcagagt tgagaacagg aggaaggttc tgaggagccc 114180 ggagggtgga tgtgggtgac aacttcagaa gaagaagggc aggctggaag ccagtatatg 114240 gagacagatg tcattcctgg acctcacggc taccttgctg agctcacttt ctgccagcag 114300 gagaaacatg ccctgaagat gtgaagatgc cagagaccgg tacagaaaag cgagaggagc 114360 gttgttgtgg ttggttgtat gtgctgactt gatcaggctc cagggtgctc aggtatttag 114420 aaaagcattt gtaatggctg tgtctgcaag ggtgtctttt gatgtgtgct accgtgacct 114480 ggcagagtga agtatacatt gctctcccca gccctggtgg acctcatcca acccattgga 114540 ggcctgtgtt gtgcaaaatc gcagaggtag gaaaatgtct tccctctgcc tatttttgag 114600 cgtgagcgtg aatgctcttg cctgtggagt aagacataga ctggacctta cctgtgttag 114660 acctccatgc ttcttggatt aggatttgga ctagaattta tcagctgttc tgggtttgct 114720 gttgtgaccc acaggtcatc acactcctca gcctctgtag cactgtgaga ccattagtta 114780 taagaactcc tggagttgga gagatggctc agaagtgaag aatgcttcct gctctttcag 114840 agaactggag gtcagctccc agcagctgta tcaccatcac gtgacttaca aacacctgta 114900 cctccggttc caagggatca agtgcccttt cttgcctctg tgggcaccca gacatgtgca 114960 taaacgtaca cagatataca cataaataaa gtaaaataaa ataacatgaa tcttaaagga 115020 agaaacccag tcaatcatca cccatcctgc ctgcatcctc ttggttctct ttttctctgg 115080 gtcaaatgga ttgccccata caagcatctt cttgctgcct tctctacagg ctggggctaa 115140 acagggaagt tatgggtggg gacaactttc agacagatca agaaggagga aggagcgact 115200 catgattttc aaagacaaca caaatgttgg cagtttgggg atgtaaccga tgagggcagt 115260 ggactcaggg gcagacccgt gcgcatgcgc ccacgtgttg ccatggcacc aacaccatgc 115320 gctcaatggg atctagccac acttacgtta atgctctggt actacagagt tggaactttt 115380 agtaagtttg agcgaggagt aatatatcag tggtgatgag catgcagggt taggtccctt 115440 agagaaatgg tgactagggt gggaagtgac acagctgttg ccatatcaag gctagagagt 115500 tgctccaccg aatagccata agtcctagta tcagcctcag agttcaggaa ctctgctttt 115560 cttgtttgtt tgtttgtttg tttgttttct tcagacgatt ctgtgaaggg aagatggaaa 115620 gtgttaatta tataaactga agacgtttcc ctgtgaagta aggccagccc atacccagcc 115680 ttaggctaag tttcacttgc cctttcgcaa cacaaacctt tgtccctaga tctgactaag 115740 aggttaagca atgtgaatac cggtggccag gcccttcctc tccccagaag agagaagagg 115800 tccccgaggc agcagatgtg agtcaagtgc cttgtggtag aggaggccac tggagtagac 115860 ctgggaaaag gttctgacac tgtttatgac tgttgggctg ggcttgatca gaccaagcac 115920 caaacgcaga gctatgtatg cgcagctctg accttcctcc agggaggatt tcaggatggg 115980 tgctcatgag gctccactcc tctatgagta tcggtaggca ggattaatgg ttgccccaga 116040 aaagtaatgt tttcttcagt ggtgtagcta ctgataagtt gcccatgtca ttgtaaataa 116100 cccctcactc aggctcttgt gattaattca ctggggcaca tgacatcaat gacatcacac 116160 atacacacac acacacacac acacacacag agagagagag agagagagag agagagagag 116220 aataataaaa aaataaaaat ttaaaaatca tctagtcttt gttaataagg ataatatact 116280 tggtggtata cttaaaattg gtggcaaaaa atcatgcccg taagtaagca ctgtctgagg 116340 gcttgcgaaa gtttctaaac ctgtagttac aggcatcctc aacaaaaggc atggcaagtc 116400 attgacatta gtgtcaccca ggcacacagc caactcaggt gggaattgtt ggctctgtaa 116460 ggacaggcaa aggaaaaggt cacactcaaa tggctcatct ctgtcatcct tagtagaaaa 116520 aattgaaaat gatattagtc aagaaaggac taaataagat gatatgccta atattcaggg 116580 agaccaggat aatgtttcaa atgccctgga ataactggct ctgtgtgtgt gtgtgtgtgt 116640 gtgtgtgcgt gcacgcgcgc acgcacatgt gagtgcacag atttatgtct gaatggtctt 116700 cagaaattag gtgatattcc acaggaagga ttttccatcc tatcaagaac ctaagaatat 116760 ttcaaggaac cagatcacta tttagggaag agggaaggtc gtgtattata ttagccagat 116820 atacttcact cagatatgat tttggattac acatggtggt gcacaccttt aatcccagta 116880 cttgagaagc agaggcagga caggagacac ccagggccaa cgagacccac atgaatgcac 116940 accccctcat gtgtcagtga gagctctcgc acactctccc tctcataatg agaaatgaga 117000 aaaggagaaa gataaaatat ctgctattcc ttgtattagt caaaaaactt cctgcgtttg 117060 aggatagctt cgtgtgtact tagatattta gaaagtctca ggtaaaccag tgcttaatag 117120 tttgtctcaa acaaacaaac aagcaaacat gatttgaacc aatggcattg ggtaggaagg 117180 ctttgggctt cagataacaa agctgaagga aggtctgctc tagccacagc tgaacgtcca 117240 gtcctcttct ctaacaggaa gtctgcagga agttgctctc caagtccacg cagcacttcc 117300 agggcgtaag cagctcagag tctctgaact tgcctgcttg tttcgctgct tcctcttcca 117360 ctccaggtgg gaaccacggc tttgagcgac agagtctcat atgtcagctt cttgaggcag 117420 gagagagaga gtgataatgc tagaggattg tcttccccct tccccccccc ccccgcccca 117480 ctctgacagg aagagccttc gccaccgtga caacctgcct gcgatcatct taacttagtg 117540 gatagaccct cagacgagtc actggctgac aaatatagac acccatgatt ggcttacagc 117600 atagtgttca ttgcttcctt tcttttccat ctgcagtcca gtggggacca ttaaaactaa 117660 gtcaaaggga aaactattca tggtttttta aaattttttt aaattttaaa gagagaaata 117720 tgaagggcta gggagatggc tcgggggtca cgtgtgtgtt gtacaagcat gaagattaga 117780 gtattatctt cagcaagaag gtaaaaaacc tggatgtggc aagacctgta atcttggcac 117840 tgggaggtag agaccgaagg atcctgaggc ttcctggtca atcagcatag agcttcagat 117900 actgtgagaa aaccaaatgg cagagaggga taaaagaaga tccccggtgc tagcctcatg 117960 gacatgcaca caagcatgca agaatgcaca cgcgcgcgcg cgcacacaca cacacacaca 118020 cacacacaca cacacacaat cataatgaga gtagagaaaa gtagaaagaa agacagttta 118080 ttattccttg tattggtgaa accctccact aaggggcaaa agctattgta caccatgaac 118140 acctgcaggc tcaacccttg ggtgcagagg gagaagactg cacaatgaat tccacaccaa 118200 ccatggagaa acataaatta acgaacaaat aggaaagcaa gcaaaaaaaa aaaaactaga 118260 ctggaaggag ggattctggg tcatggaaat atcattcgcc attgatgaat tttattttta 118320 tgttcatttt tcagctccat ccctactcct taatgtcgct gccatgttcc tgtgctatgg 118380 taaatctctt gatttaaacc aaaagctttc cttacttcct ttcagaattt cacttacgtt 118440 actctctctc ctctccctca cttacaatga ataatcttgg aagaaaacat gtctgcatag 118500 aaaaggactt tctggttagt tcataatgtt gttccaccta tagggttgca gatccattta 118560 gctccttggg tattttctct agctcctcca ttgggggccc tgtgatccat ccaatagctg 118620 actgtgagca tccacttctg tgtttgctag gccccggcat agtctcacta gagacagcta 118680 tatctgggtc ctatcagcac aatcttgcta gtgtatgcaa tggtgtcagc gttcggaagc 118740 cgattatggg atggatccct ggatacatca ctggaaagag aggcccattg gacttgcaaa 118800 cttgatatgc cccaggacag gggaacgcca gggccaaaaa gtgggaatgg gtgagtaggg 118860 gagtgggggt gagggtatgg gggacttttg ggatagcatt ggaaatgtaa ttgaagaaaa 118920 tacgtaataa aaaaatatta aaaaaaaaaa aagaaaagga ctttctacct tactggcaac 118980 tgtaggctct taggcgaggg ctctcgcctc agtcatacgc gtcttcctct ctgtaaagtg 119040 agctggctaa attagacaca tccgaaggtc acatagcccc agttctgaaa cttgatggtt 119100 ctaaagtctg cggggacaag cacatgaccc ttctccaacc tctggtatca ggttctgttc 119160 ccactcgttt tattcattat ctgctctgtc ccctacttaa cccagactta tttgtgcagt 119220 ctctgaatga gcaagccagg cttttttttt ttggatgata ttattagatg ctcctgaata 119280 ttctccaagt gctttaaatg aaaactctgg acagtacagc cagacttccc aagttgccaa 119340 cttgtcccag gccttagaag tcacctaatt acattgaatt ctgcctggtg gagatgaaga 119400 cagaaagcct gtcactaatg gtgcatgtgt tgagggtggt gaccactcca tctttattgc 119460 cagctatgct aaagcccttg ctgcatcaga aggagtgtga gcttgtaaag ttccgagtct 119520 gcagcatgat cttccattct ctaagcggta aggcttaatg tgtgtgtgat gctgaccagt 119580 gaaggaagat tctagcaata ttctgaaagt gatagataag aaatcaatac taaaatccat 119640 ggctgagaat ccgtctttgg ttaatgggta gccacagaag gtgggagaga gatttctgga 119700 tacttcaagg agcagaagaa taagatcagc cagaggggct gctctgagca tcgattgctg 119760 ctgctgctgc tgcccctgcc gccgccgccg ccgcctttgt cgtctttgtg tattttgtgg 119820 cgctaaaaga atagcaagca ccaggcagct tctaatgaac aaatgcttgt gattctggag 119880 gccaaatcca aaactgagat actgacatct ggaggggcct tctggctgtg tctaatatcg 119940 tagacctcat gtggcagaac cacaaatgga gagagaaaac taaaagggtc cacactcact 120000 cttcactagg aatccactcc tgcaatggtg gtcgtaatcc cctcacccca ttctttcagc 120060 tgaatctttt ttttttttca tcaggttctg ccgcccaacg ctattgcgta gggagtactt 120120 ctagcacatg cttatgggag tgtgttatca tcacaagagt tggtaacttt caaggagaca 120180 gtgtgaggga aggctgtaga aatatttaag aggcaacaga cttggtcagt caatgaagcc 120240 agaactctaa ggggaagtgt gatgctcact aaaggaaaca taaaggactc cacccctacc 120300 ccccaccccc gggtgaacag aactcttctg tgctgtccca gccttgcaca acagcaacac 120360 tgatacccta ctcccacagt gacagaaaag gacttgggac tcctatgaag gacgagggac 120420 ttgactagag ctcactacct tgacctttca ctgttagaag tggcataagg tgaagagcta 120480 cagcttaggg gctggagtga tggccacttg ctgttcttcc agaggaccca agttcagttc 120540 ccagcaccca catcaggcag ctcgcaacaa ttacccatga ttgctaccat tgatgctgat 120600 gccttcttcc aacttctgag ggtacctgca ttcttgttct ctctctctct ctcatctgta 120660 aaaatcatat taagaaaata tatatttggg tttaaaatca aaatggaaga tacaggttat 120720 agagatatat ttagcaaagg aaccaagaac acttgatttc tgtgccagag agtcacactt 120780 tcctcaaaga gctcccaagg agaaacaata aaaataaata cagctcttac tttgcactag 120840 atgctgttag gagcacttta actccattag ctgattttgt tcttataaca gatgccaggg 120900 caagagaaag ctgtatcagt cacttgagaa ttgaagttga tttcatggca ggcggtggga 120960 cagagagagg aaagtgggct ggtccttaca aagcacagat gcagaaaagt tacactggga 121020 gacaaaaatg agaagagatg cagagtagcc tggctagctg gctgggttca ttcatttact 121080 cattttacct ttattgagat ggttagaggg taagtctgtg ctcaccatag tgacaaactt 121140 ttaaatgatg cttgtgcttc agccccctcc agtatcacct cagtcttatc attcgaacac 121200 ttggtcctga cgggctttgc tttttgacct tgtacatttt tttaaaaagc gatttttatt 121260 tctttttata ttatgcctgt gtgatgtaat tgtcccacat gagtgccacg gccacgggag 121320 cctgaagaga gtgtggcatc ttctggaact agagttacag atttgtgagc cctgtgtggg 121380 tgctagaaac caaatccagg tcttctgcaa gagcagcaag cactcgtagc tgatgagcta 121440 tctctgtagc ttccggataa ttattttgat gacaaatgtc tacctgagcc tccatttata 121500 acattgcaat cattcctaga gatgtgcact agtagatttg tacaatagag tgcaaatcag 121560 catatttcat gaagacttaa aagcttaagg tccttcagcc atttggaatg ggcaaactgc 121620 tgggacattt ataccatgca gtgttgtata gcagagaaaa tgggtgaatc atcattacat 121680 gtgagtgaag ggctggagag atggttcagt ggttaagaac atgtactgct cttacaggag 121740 cttagtaccc agtaccatgt caggcagctc acaactgcct gcagttctag ctccaggcgg 121800 gaatcgaata cctctggcct ctgagagcag gcatatacca ccatacacat atatgcataa 121860 tttaaaatga aaataagttt tgaaaacaat taaaaaataa caggatgaat aaagactgaa 121920 caacatgttt gagtctatgc cggggcctag caaacacaga agtggatgct cacagtcagc 121980 tattggatgg atcacagggc ccccaatgga ggagctagag aaagtaccca aggagctaaa 122040 gggagctgca accctatagg tggaacaaca atatgaacta accagtaacc cggagctctt 122100 gtctctagct gcatatgtat caaaagatgg cctagtcagc catcagtgga aagagaggcc 122160 cattggactt gcaaacttta tatgccccag tacaggggaa tgccaggacc aaaaaggggg 122220 agtgggtggg taggggagtg ggggggtgtg tatggggact tttgggatag cattggaaat 122280 gtaaatgagg aaaataccta ataaaaatat taaaaaagaa aaaaaaaaga ctgaacaaca 122340 aaaacaaacc agaagttcca ggagaatatt ggctccgcac tttatctttt ttaaggtcca 122400 aatgccaaaa caggtattta ggtagttgtt gtctaagcca tttgttgccg atttaagtaa 122460 aagtccatcc attttctgtg cctgctaaaa ttgatacttt taaagcaaga aaatgaaaca 122520 ggaggaaatt caaggtggag gatgtctcgg tggagcagga gggcgtgaag agagagcaca 122580 cggaagtgag gctgttgacc gtgttctcac tgtgctgggc agttgagaca tagatactta 122640 tttggaaaag aattaaactc gagggactca gtggaatgga aaaagtcagc tatgggtgga 122700 acaccagacc atttcatgaa ctgggacttg taaatgttct cattctggca cctgctgagc 122760 tcctagtgac ataagagacc taagctccag atttattccg aattaacaaa ttgagccaga 122820 tcctcagatc tcgtggaaga acaagcaggt gctctcgact aatgagccac ctctccagtc 122880 ccaaggtata gagctttgat ctccattttt ctttatgtac aaatatttcc ctaggcatta 122940 aaggtgtaat ttccataatc tattcagtgt atcaggctgt ggtcatactg aataagaggt 123000 tttcttcaca gaagcatcct gtacattaaa tagaaagatc aattttaaat gacacctgag 123060 cttactccag tgtctctgct aatttgttga acttttcctt cataatctct ttaaattaat 123120 tattaactag cccaaagcag ggtggacttg ggcattatag agtacctggt caatttagaa 123180 atcgtttagt ccaagtcatt agaggattat tacccttttt tttttcttca gccagatggg 123240 ggatgtcata aatgtacaca attagggttc tttaaattag gcttatggct tggatgtgaa 123300 atgtccccta gaagctcaca tgcttgatta cttggtcccc agctggtagc actctttcag 123360 gaggttggac agacacacag tctcattcat agttggtggg gcatagggat gaaacttgag 123420 ggttacggat cgcctctgat tctgtcctgg ttgctctgtt tccagatcca gtcacatgtg 123480 agcaagctat ctcacactcc cgttgtcata acggactggt tctgtttcca tgtgtttctc 123540 atggcggtga tcgactttat cctctgaacc tcgtgcaaac cctccatgac tcaagttggt 123600 cctcctatta ggtgtttgcc ctctgtcatg agaagagtga cacccatagt aggctccttt 123660 ccatccatct cgcatgtgcc ccacggtcag gttctgttgg tctaaggctg accatcaact 123720 gctgctgaac atatatatat agttgagaaa cttttccagt catactttcc aagaaataaa 123780 taggagcttg agtgtggtga gctgtagctc tcagctggcc actgatgtaa ttattaatca 123840 ctgcatgcaa ttaattggga cctttcttag acatgtttgc cactgatccc tgtttccagg 123900 gagtttcaca aatgttccca ccccagcctc gtacctctat ttgttctttt gtaactttat 123960 aaaaatttta atctatacaa gtttgcaatg cattgatcat atctattccc accctcacct 124020 tcctggttat ccgagattct ccccaccata tccctttgaa gtgtcagatc ttcctcttct 124080 gctctttata acctactaag tggtgttcat atgggcacag gtgtaggaac attcaccaca 124140 gcatgagcaa cctgtaagag accacattcc tgaagaaaac tcccctttct ccccagggga 124200 catcaacggc caataactct cagttatgga tggggtacct tccgtttatc ctggaatgtt 124260 gactggatga atctgtgtaa gcagccactg ctgctgtgag ttcgttaagt gtaatggcct 124320 catgttgaag ttaagccctg gatcttcctc caagctccgt gctcccggaa ataaggctca 124380 gactcaaaat atatttacaa ataccttggc catgtaacta ggctcttctc tgactagcta 124440 taacttgaaa taaccattga ttctagccta cattctccca tgtgattggc gatatgagct 124500 caggtaccag gcatctgtct tgtcacatct tttcagtgac tctctctgcc tccttctctc 124560 ccagaattct ttctcctccc acggttcacc ttctatttcc tgcctaagcc atagcccatg 124620 ggctttttag ctgacaggtg atgcattcat acagtagacg agatgttctg tttatagcct 124680 tgccataccc agaagacact gtgtcacaac agtcactccc catcctgacc tgcagccctt 124740 atgacctttt ggccctctct ttcacagtgt tccctggggg gtggtaatgg tggtggtatg 124800 catatatgtc ctgttttgag agcaccgcat ggtcattcca catttgttct taagtggaag 124860 aaagcatgat gcaaaacaga aggagtgagc tagactttaa ctggatttag gggactttat 124920 aagagaaagt aaaaacgtcc tcaagaattc attaaaaatg aagagcatct cccccatgct 124980 catttctgtt cattacaaac gactcaacta ttacatgcaa atattactct aggaaaatag 125040 ccacccactg atatgggtgt ggagatctcc aaagacaaga ggaagttgaa aagggctcag 125100 atggctcctc caatgtggcc ttccagctta tgacaagaga aacacaattg ctggtccagg 125160 gacagatgtc tgctgcactt gctttgttcc tggctctccc tctgacccaa tttacagccc 125220 ccctgcagct gtctcctgca tactttccca cgtgaagtta tttatctttc ttaacagtca 125280 actcgattta ttccaagaga atgctcccct cccccagctc tgctggtaat ctattcctgc 125340 actaaattgc cattgtaatc aaaattccta gaatagttta agttggcctt ctgcagctgc 125400 agtcacctct gtcaaagccc tggccaagcc tcctcagtgc cctggagtga gtgtcattcc 125460 acattgctgg aaaccacacc ccatctggct cttcccgcct ttccaggctc cttttgctgt 125520 gtccttttat catctctact gaaccacttt cctgacaaag ctccaacacg gttcctcctc 125580 tagagctacc gacctcttca actcctatgt ggtcattttt ttttctgtcc ttggccattt 125640 cttaacccat ttttttagga catcctcttc tctccttgaa acttggtcag atctgccacc 125700 atatgtacca cttactcaat attttgatcc cggagctcag ttccgtggtg gctaattttg 125760 tcaacagaca acatctggaa tcatctaaaa ctcaagttgc tgggcacttc tgtgaggcag 125820 ttttttaaaa tcagattatt tgaagcagga agacacatcc gacatccggg ccccactttc 125880 tgatggcagc ccagataaca tgacgtggaa gaaggacact tggctttttt tttttttttt 125940 ttttgcctgc ttgccccact gtgtctggca tctctcctgt tgctcaggca ttccttcact 126000 attcagaatc ccaacaaaga tagaaaacca tctgctctcc aggaatcctc caggatccca 126060 gagctaggtg aggactgctg agacacccag ccttgtggac tgaacagctg ccagtttaga 126120 tattggtctt tccatcatga gactgccatt gttgaactac tcggaccata tcctgtaagc 126180 cagccttata aatgcataca cacacacaca cacatgcaca cacacacatg cacacacaca 126240 cacacacaca cactcacact cacacatgca cgcacgcaca cacacacatg cacacacact 126300 cacaaatgca cgcacgcaca cacacgcaca cacacacaca cacatacaca cattcactct 126360 cagttctgtt ccttcagaga agcctgacta aatcaggttc ccagcaccta tgatgggcag 126420 ttcacaatgg cctgctaatc tagctctggg agatctcctt gcttcttctc atctctgcag 126480 gcactgtact cattgtgcac acataaatac taatactaat aataatagta atgaaaatta 126540 taaattcata tgcataccag tttttattag ttggcaacag attgcataac cacaacacca 126600 cttcacaacc agaaaattga cagactatac tagtcatatc caggtttcat agttttgccc 126660 tgtgcttaga catgcatatt ttcagctatt cattgattgc ttgccatact agaaactgac 126720 ctacacaggc taagccagtg ctctaccgct gagatgtatc cccagccttc tggttccatc 126780 tgacttaagt tgcccaaggt agcgttgatc ccaatctgta acacaggcat acctgaaact 126840 tgcaatcctt ctgcctctgt cacctgcgta gttgagattt taggccggtg ccactctgtc 126900 tgtaactttg ttatgtggag agactcgtgt gcctctctat gagatacact tgggaagaaa 126960 gaacctgccg gttaagggat ggcctccatc aggttgccta tggggatgtg tgtggtacct 127020 tctctgtcca gtttactacg gggagcatct tccctaggca ggtcgcttag gttgtctaag 127080 aaagctagct gaacacggag caaaccacta aagggcatcc ctctgtggtt tctgctttaa 127140 ggtcctgctt tcaatctgtc tcctgtcaga tgaaacaaac cctttcctct ccaaattgcg 127200 tttggtaatg gtgtttatca tagaaaaaga aaaccgagaa aaagaaaacc accatagtca 127260 aacttagact cgtcccatca gtggcctctt gtgatacctt ttagaaccaa ctgccacctg 127320 tcccttctcc cgacctgtga ccagcaataa tatgtctcca gatttatact ttgattaatt 127380 ttaagaatgt tgcataaatt ttatcaaaca ccatacggaa gtctgaaatt tccctcccca 127440 gcatgattct cttgatatcc acctgagttg ttgtgtagac cgacactgtt ctcattgcta 127500 ttggtgagta gtatttctga atgtggactt ggtagtttgt ctaattgctc gcttgttgtg 127560 agatgcttgg gttatttcta gttattgaaa atcaacatat atgaaaatgc atatgtagat 127620 tttgtgcact ttcacttctc ttggataaat gccttaggaa aatgttttct gggtcatatg 127680 gcaggcatat gtttgatttc ctagagacat caatgcagtt tttagactga ttgtcccatt 127740 ttacattctc agtagctgcg tatgagtctg acatccttgc cagtgtttgt tgatgtcctt 127800 aaattttttg aaccattctg atagtagggt aggatctcta ttttatttat atatgtgagt 127860 atgtgtttgt gtgtgagcat gcgcatttgt gcatgtatgt gtgtatgtgt gtgtgtgtgt 127920 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gcatgtgtgt gtggaagacc atcctcaccc 127980 ttggagctgg agttataggc aattgcgaac cacatgagat gggaagtgaa ctcaagtcct 128040 ctggcagagt cctctgtgcc cctaaccact gagtgacccc tcaactcctt agtgtatcat 128100 actgtatgtt tactgagtgt ttctatgatg gcttatgctg ttgatcctct tttcatatac 128160 tactttgtca tctttatagc ctctttagtg gcagatctgt cttttctaag tttcctagtc 128220 taatatttta atcactgagt tttgacggtt ctttatatgt cttagatagt gccaacctcc 128280 actctgttta gatgagcaga aggtctatgg ggttaaaagg ctttttcagt catttagcaa 128340 ccgagaaagg aaagatccaa gtctgcccct gtctttatgc agtctttacc atggctcatc 128400 tcatatgtag tgtcttccca actcatcaag ctacattcat cttagcatac atagtctccc 128460 aaaccaaaaa cccataacca gggtatacat ccttttggtg gggaattgtc tgaagtttgt 128520 cttctaaagt gtccagcctg tgttagtcat tgtatcgccc tatatagtca acaggggtgg 128580 ggatggaaca caacacctaa gtatcccttt agccccgaag agccttcagt tgtcactcct 128640 catcaaaact atttaactag agtcatctca ttcaaattca agtccccttg ccagattaaa 128700 atatagctga tgagtagtta tcatgaaggt aaagaagcca gtcacacggc tagggagatg 128760 gtcaatgggt aaagtgcttg acttgtaaac cctaggaccc aaattcatgt tcccagcaac 128820 cacataaagt caaacgtggc atcttgtttc tgcaagccga ggtgtgaagg gtgaagaagt 128880 gaatcccagg gacttcgggt gggtcaatct agttgaagcg atgagttcca agtttagtga 128940 gaaacaacgt caaaaaataa ggaagaaaga aattgaggaa gacattgtga tatcatcctc 129000 tttcgtgtac atgcacatgg gtaggtaagc acgcaccact cacacacaca cacacacaca 129060 cacacacaca cacacacaca tcaattcttc atagctctaa gtggattctt tttgtcaaag 129120 aatcagctga agcttaatgg agattgcata cctgagctct tgtcttctac agggattgta 129180 agagcaatgc ccagatgact cttatgtaca cagctgtccc agagtctgtt ttctaatcac 129240 tcagcctgtg aaaactggtg ccatccatga cttgaacagg ctggtggtct tgggagccag 129300 atcactttcc cagtgggcac tgaggagctc cacctgccac cagatagcag agtactgatt 129360 aggccttgaa ccagaaatga ccagaaaagc atgttataga tgagaatagt acagtggatg 129420 tcatgttcta ggtgtctgag aaatatggag gaaaatgacc ctaatcataa agaaattata 129480 gggtagatat taagcacgcc taaattatta accagacaat aagaggacat gagagattaa 129540 ttgaaggtta atagctcatt gtgaaaggca ggggcctctc tggtagctta taaagatgct 129600 cttggctttt gcaacctgag gcctgagaaa ttgtggccta tgacttaata gtaagaatgg 129660 tggaacaaca atgaagggtt aagacctgaa gtgaggccat gtcataagtt gttgggccat 129720 tgtcctcacc caggttagga aacaatggaa tttgacacat atggcaggag tggattatgt 129780 caaaaacaat aaaactgaat tgaattaact gtattggatc tagagttgtt tttttttttt 129840 tttttttttt aaattctgtg ctgtaagctt ccagaaaagg catactcctt cctgttaaac 129900 accagtgctc ttctgatgta tcaggactgt attagttacc tctctgtgac tgtgagaaaa 129960 caccatgaca aaggcaattt gtagaaggac gggttttact gggttatagt tccagagaga 130020 taagagttca ttatggcaaa aagagaaggc tggagatagg tatgggagct gagcaggaag 130080 cgcaaaggcc aatggcaaac atgaagcaga gagaacaaat tggaagttgg gtaaagctca 130140 atgcttgttg ttagtgatgc acttcctcca ggaaagttat agccccacac tcccatcgcc 130200 tctccaaaca attctgccaa caggggacca tgtgttcaaa tgcctgtggg agatagttca 130260 cattcaaact accacaaaaa ccatacagtg ctccatgagt taggaaaaca tgcctgcttt 130320 accagattct ctcctggtca ccaagctaaa ccctaaggat atatcaccac aacctggcag 130380 aggactgagc ttggtctggt aatggaagaa ggtataaacc aaaaggaaca tcaggtccta 130440 gccattacca taagtgggac tggattgtgt aagttttgcc tcaaaggtgg cattattcta 130500 agttagatga tggaggatct gtttcttgag gcagggtctc cttggataca ggagttacca 130560 tctatcaaga taggataaag ctgtggctgg catgaataga atcatgatag atgatttaaa 130620 gcctatgtgg gggatggaag cagcctcccc taaagtaaaa gtggaaatgc attattgtca 130680 ttgctatgga tgctttctgg gcaataaaag acagtgggat atgacataca gctagaagtc 130740 ttatttaata atttctatta tgatgctata atgcaaagca tggagatacg gacagatacg 130800 atgagtactg gtataattaa gaaattcagc agtaaatagc tcctgaagtc atttctaatc 130860 agagatgtca ttgtggagat tgtggagatt gtggattgtg acacagatag gaagatccca 130920 aattaacagc cgcaggggtt ccatttcaaa cttagatgcc aggtgacagc atttaccaaa 130980 acagaaggtc aagtgcaaaa gaacaatcag gagatctgac tccaagataa caatggcaat 131040 tgtcaacaca gcttcctagc gttggggaga aaagagatgt caagagacaa taaaaccatt 131100 gcacgcacca gagcaagagg agccaagagt gtatgagcaa gatcctagag acaaggtttc 131160 tccacagtgt gttctgtcac ccaggttcag agctggccca gttttcaaac acagaaccca 131220 acgatgaagt gatcaggtgt cctggaaaaa aaaaaagatt tcttcaaaac catggtgact 131280 taacaggtaa caattccctg acacttccct gaagcaatct tcaatcacca actcagttag 131340 ccataagtta tgggaatgtt tggttccaaa catgtcaacc tgacacagga tcatacacag 131400 attcaaagta tcaacatgaa ccttctgtta cactgggagc acacctggcc atgaatggaa 131460 gctcagaaca gtttcaggat tacaatgtgt ccacagagta ggtaggctca accagtgatc 131520 ctctctcctg tcccagaatg taacattgaa gaagtgcact tggcattggg cccagcttgg 131580 gttcctttac ctgtggatga aacttaatga gggaaacatt aataacatta ttaacattaa 131640 taacataatt aaaacataat gaggggttgg acagaagcac caatgagaac cctgttgctc 131700 aaatctgccc cttacaaagg tataaaatgt gtgttctttc tttgttctgg gtctctcctc 131760 tggcctgcgt gctgagaggg gagtccccaa catgttggat caataaaaat ccaataaggc 131820 cacactccta atagtgccac tccctagccc aagcatatag aaatcatcac attccagtct 131880 ctgaacccca gaggcttgct caaacatatg ggacataact aaacctaaca taatgcaaag 131940 ttcatttagt ccaacttcca aagtcctcat agtctgtggc ggtctcaaca atgttttaac 132000 ttaatctttt aagaccaaag ttcaaggtct ctactgagat ttactcaatc acttaactgt 132060 aatctccaaa gcaagacagg aaaccaaatg ggtaaactcc aaactttgga tcttcatgtc 132120 tgatgtcaaa actctcttca gatctccaac ctctttttta tctttgttga ctgcaacaaa 132180 cttctttctc ttgggctggt tccactccct gttagcagat ttcctcagca gatagttcat 132240 ggctctggca tctcaaacat cttggggtct ccaaggaaaa ttcaatgtta cagcttcttg 132300 tttcaatgtc tgggatccac acataatctt ctgggattct ccaaagggct ggtgtcactt 132360 ctccagcact gccctcggtg gcattctaag ttcagagtga tccactccac tgttgctgct 132420 gttcttggtg atcatcccat ggtactgtgt tgtgtctgct cttgaccagc aagaaagaca 132480 caacggccag attcttctca acagccttta ttgcaggaat gcctcaatgc tgctacaggg 132540 acctagggaa cccagggagg tctgcttata tactctccag cacagggaaa ggcttcatgg 132600 ccccctggga ttggtcagtc tgccggcacc taatttgcat gcacccgctc cggaggggtt 132660 ggcgccagac tcggacttac atctgcgctg atgttgttta tgacaatggc acacctggtg 132720 cccagagcca tcttagagtt ggcttcctac agtactggta tctccaatac tctggggcct 132780 tctactgcaa cgaggcttca ccaataacct ctcataggtt ctctccatgg tgccaagcct 132840 caacttcttt tcatgacccc ttcagtcctg ggctgtcaag tgcaactgag gctgcatctt 132900 caccaatggc cttccatggc ctctcagagt gccaagcctc agctgctctt tatgttccct 132960 tcatcccttc aaaaccagta acacctgagt gatggggatg gatgtagatg aggtgaaacc 133020 cagaacacct ctcattccct gcaagggaag acccatgcct gtagccagaa atgttaggat 133080 tagagcaaaa cccttagtag aaactgctgt gattctcatt tggaagacca tggactgatc 133140 tgtcagcagt gttattggag gtagcctttt tctttctcca ttgtgaagtt gatggtcctt 133200 gggcagactt caagaaaggg tctgaggaga cagacccagg aagagtgtag caataacatt 133260 tgatttgcga ttaagaaggc tggagtcata attagcccta aatgggacaa ctctatcacc 133320 ctctccaatg ctcagggaag aggagacaga aagaatgtaa aagccattct gggcttgaca 133380 tagctgttgt cttcatgtgg tgacctgcac tagagctgca ggcgactggg cattcatcaa 133440 gtgtgtggga gggtcccctg aggccccacc cctcccttca ggactaacag aagttagtgc 133500 ttgccaaggg agtagctatc atcctctgca gtgacacgga agctgacaag ttacccatgc 133560 tccaggaaat aaactcccac ccacgctcag gcaggcgact taccttagac tgagtaagtc 133620 acacacaaaa gaagacatga aagaaggagg ggctcttcgt tgagactaag ggtttcaagt 133680 gggagaaaga gaaggataag actattaaaa ttcattacat acaggtatgg aactgccaaa 133740 gaagaaaatg atgaagagca gcagctgccg ccaccgggag tgagtcagag gtggcctgca 133800 agcctgcagg aacctggccc tcaccaacag aaaaggcgga ggacagacac ctgacaagag 133860 tgttctcttc cctccactaa gtgtctttta cagtttacat gttgatttgg gagactgtta 133920 attctgggat atgcgggggg tggggtgggg ggaacaactc caacattctg gaccaaattt 133980 aaagcaacct ttattaaata ttagatattg accaagggat ggaccttagt tgggaccatt 134040 tcacagaact tcccacctga gaatggcctg agccatgggt tgcaggggca aagggcaaac 134100 ctataggcca ctgtactttc ccatgatgct ttgctgtttc ctaagaacta taattcccag 134160 cattgcagga agttactggg tttctgggca ggtaaggctt acaggttaat tttgggcatt 134220 ataagacaaa taccaaaata ttatctatga tactattgct ttattttatt ttattttcaa 134280 ttacatatgc ttgtatgtgc atgagagtgc aagtgcccac agaggttaga aggaggtatt 134340 aggtatcctg gagatggagt tacaggagat tatgagctat catatagatg ttggaattaa 134400 acctcagtcc tctggaagag cagccaatga gccatatctc cagccctatc aacctgttgg 134460 gttgtttaaa aataattctt ttatttttat ttttaagtat gtgtgtgttt gtgtgtgtgt 134520 gtgtacatgg gtgcagtacc ctaagagacc ggaagagaac attggattcc cccccccccc 134580 accgccccca cctcccgagc tggagttaca ggcagatgtg tcccttggaa accaaactca 134640 gctcgcctag aagaggactg gactatcgag cctaagcgcc catctaagtc tttgtatcca 134700 gtctttctgc aaccctggaa gcctttttac caaaaagtac attctttcat cctagctgtt 134760 ttttgtgact agcacctgct gaaaaaaaaa aataagaaag aaagaaagaa agaaaaaacc 134820 caaaacatca tcaaaacaac aaaaagccaa gcccaccaac aagaaacgga tatacacaaa 134880 agccaagaaa aacaaaacaa gcccccccaa gccccagcta ttcatcaaga taggaaagag 134940 aaacacccaa ctcctacctg cgcttggtga ttgcttggtg aaaaataaaa gtatttttcc 135000 tctcattcct tcttttgtga tcaaatgaat aatgaaggga ttgagccttt gctagtgtgc 135060 atgcccctgc agggagccaa gaaacaaata ggtttcctaa gggtataacc cagccttttc 135120 ggcttttccg tcatcacgtg attgagggct tctgcttccc acttaacctg catccttccc 135180 agactgattc atggaacctc aagggctcta caggaaaatt caaacatcag cttctctctg 135240 ccataggact tccatccgaa ggaatggttc cttggttgtt gccttcacag ccctcacatc 135300 cgggctggac ctggcagaaa gttgacattt gacccctatc tagagcatag acgatgctgg 135360 cagccgtatg tcctcacttt tgccattccg gtgcctccct cggggcacag ccaaggttcc 135420 gagatgggca ttttcaggag gaaacgattt tatttgaggt cttggcaggg agatgagagg 135480 aggaggcgtg ccaccttctg taacaaaagg catttcccag ccttagacac acccttaggc 135540 ccagatgtac actcccactc ctgtcaatcc aactgcactg actagcaacc ctaatgcctg 135600 ctgcttccag gcaccccgca gcctgcgtct tgtttccact atttcctctg cctgtcgcag 135660 ggaaagcctc actaccaaga gccttcaaac acagttcctg ttactaattt tgccccaaag 135720 ggtaccacag tgattaactt aatgcttccc cagggatgac tctctctgtc tggggtcagg 135780 gtggggctgg atggattttc tttattaagg atggtgggaa ccctcttttg agctttgaag 135840 tctcgcctta gatttttttt taattttatt ttttattttt gataactaca aagggacagg 135900 aagtctcttt ttactcctct gaggcttgct gttctccagg ggcatggaaa atgtacacgt 135960 tgttagcccc ccaagcctgg gggctggggc ccgcgctctg ccggattgtt tttatatccc 136020 agcaaaaagc atctgtgtgc agaacagcag ctggtctgct gctgccaact gcacgttccc 136080 agtaccaatg taagcacttt ccttagaggt taaaaccccc aaggcactgt ttgtgtttgg 136140 agaagaaatg ctctaaaaat agtaagtggg ggtttaaagt ctcttcgtca gatgttcgga 136200 agcacgctga cctggtgcgc tgcttcttag aaccagagct cattggcagg ttgctgtgcc 136260 ttgcacctac gattcctgac tccttcagta agctagctgg tgaagagctg gagaatctgt 136320 atttataata aatccccggg tggggcattg atgttgctga ttttaagaac acacactgtt 136380 gtagccagtc aaaagaccct tactcagaaa gaccacagag accgccgcca tcgctgcaaa 136440 ccgcatgagg atttttattg atccaacatg ttggggacac ccctctcagc acgcaggcca 136500 gaggagagac ccagaacaaa gaaagatcac attttttata cctttgtaag gggcagattt 136560 gagtaacagg gttcttattg gtgtagcaag taaccctttc aggcattggt tggtttgtat 136620 agtgactaag taaccctatg gcccagtgac tactttgctt gcccccatgg tgggttatta 136680 tgatttggtc agcaaagtaa tagtacttta acatcacagc tattaacgtc aagggaattc 136740 tcacaacaag ctcagggtgg tttgtggtct ttgtcagaat tcagtgtggg gcaggggtca 136800 ggagaattgc taatcttgtt atggttactt ctctgagaac agctagtatt gttttggtag 136860 ttgcaagctt aatcattttg accaccaaaa ctatgttttt acatttgttt agtcagccgg 136920 cttccctatc tggctctgca cgtggcctgc aagtacagtt tggaaagtgt ttttcgaagg 136980 aggaaggtac tgggtggtct tgaattcatt ttggatatta caacacaaag aaccactggt 137040 ctggtgtatt tccccccagt ctgttgttgg ctgcagggtg taatacagac acagggttta 137100 ttaccaggga gaggagggta ccatagcttg atgcaattcc ttattacctg tgtcgccaag 137160 agcaagtagc ttaaccttgc tggtctctgt ttcctccctt ccttgacaat catttctata 137220 gagctaattt atatcaggat cttaattatc ttgttttgtg gagggcacat gtttataaaa 137280 cgttgatctt tctccctacc aatatttttt ctcattgtca tttaaagagt cagttttggg 137340 actgacaaga tggcttagtg ggtagagaag gcttgccacc aaccctgatg gtgggagctc 137400 aatctttggg actcacaagg aagaaggaga aaacagactc tgatctgtac acatttaaat 137460 tggcacgtga gcccttctcc tcaaataaag tcacacacac acacacacac acacacacac 137520 acacacagag agagagagag agagagagag agagagagag agagagagag agagagagag 137580 agaaagatac acacacacat cccatacaca ttttttggcc aggttagaca atgctgggga 137640 aagatatcag atttatgcat ctctctctct ctggactgag ttggtgactg tgggaaggga 137700 tgggacttga ctactgcaac ttgtcagagc acagtaggcc actgtgaatt gcagctctca 137760 gactcctggt accatcccat agtccagcac atgggagcta aaagtccaaa taacaacaat 137820 gctgtctgag gacttggagg caggtgacat tgtggtaggg atttgtggat ttcgatgtgt 137880 gtttgttctc tgtttttaac accttccatc agataagttc ctgtcctgtt tcttcagatc 137940 ccaacctgtt ttccatttcc ttggggagcc tttgaaatat gtggctggat ctggatgtta 138000 tgaacttgta tggagttctg cacaagtctt tatagtttca tttgattgca atccaattaa 138060 tgtaaggatc ctccctgaat gatgcgccct gtgaaagcaa actccatgct taatctcttc 138120 cctatacttg tgtagtatgc atttcttgac ttttcatgag acattcagag actatcctat 138180 aaaagtgtgt aagtcataaa agtgtgagat taaaaaaaat agcaggtgca aatgaatcac 138240 attttcttga tccattcttt gggaaacatc tgggttgttt ccagtttctt gctattacaa 138300 atatagctgc tatgtacctg gtagaacaag tatccctgtg gtatggtgga gcatcttttg 138360 ggtacacgcc caggagcggt atagctgggt cttgaggtag aactattccc aattttttga 138420 gaaatcacca agctgatttc cagagtcgtt gtactgttgt ggccggccag cagctcgcaa 138480 cgtgaacgtg actgagatgg ccactcgagc taagggagga actagtcggg gagaaagaag 138540 aaacggagcc aagacaagtt catcctgttc aaagctcaat tttactagtt ctgacattca 138600 gttataaagg aagggggagg gaacccgatt cccgccaact ggtcttggag tccagtagca 138660 gggtgaacac gtgtgtggct ccaagcagca aagcagcacg gtccagcagt gggcgtggca 138720 gaatgaatga gcgggaaact ccacccctga gccagcaggt tccaggctgg gggtggggag 138780 gctgcagttg tacaagtttg cacctgcaat ggaggaatgt tctcttcgct ccacatcctc 138840 accagcattt gctaactctt gagtttttga tcttagccat tctgatagat gtaaggtgga 138900 atctcagagt cattttattt gtattttcca gatgattaag gacattgaac atttctttaa 138960 atgttcctct gacattaaag attcttctgt tgagaattct ctgtttagct ctgtactcta 139020 ttttttaaga ttggattatt tgttttcttg gtgtttaagt tcttgagttc tttatttatc 139080 agtcctctgt cagatgtagg gttgttgaag gtcttttccc atcatgtagg ctgccttttt 139140 ctcctattaa ttatgtccct tgcattatag aaggttttca gtgtcatgag gtccaattta 139200 ttaactgttg atcttagcgc ctgaaccatt tgtgttctgt tcagaaagtt ctcttctgta 139260 tcagtgcatt caaggctatc cccactttct cttctattag ttttagagta tccagtttta 139320 tgctgaggtc tttgatccac ttgcacttga gttttgtgca ggatgataag tatggatcta 139380 tttgcaatct tcctcatgca gacatccagt aggaccagca caatttgttg aagatgcttt 139440 cttttttcca ttgtatggat ttggcttctt tgtcaaaaat caagtgtcta tacatgtgtg 139500 agtttatttc tctgtcttcg attcaattac attgctcagt tattaaaagc aaggacatca 139560 tgaagttttc aggcaaatgg atggaactag aaaatatcat cctgaatgag gtaacccagc 139620 tccaaaatta taagtagata ttagccataa gtatacatac aggataccca agctataaac 139680 cagaaaccca attatgctaa ataacaagga gggctcaagg gaggatgctt aatctcactg 139740 agaaagtgtc ctagtcaggg tttctattct tgcacaaaac atcatgacca agaaacaagt 139800 tggggatgaa agggcttttt caagctacac ctccacattg ttgttcatca ccaaaggaag 139860 tcaggactgg aactcacaca gggcagaaac atggaggcag gagctgatat agatgccatg 139920 aaggggtgct gcttactgga ttgctcccct agcttgctca tcttcccttc ttatagaagt 139980 caggaccacc agcccaggga tggcatcacc cacaatgagc cctcctcgct tgatcactaa 140040 ttgagaagat gccttacagc tgggtctcat ggaggtattt cttcctcaag ggaggctcct 140100 ttctctgtga taacttcagc ttgtgtcaag ttggcacata aaaccagcca gtaaaggagg 140160 agaaacaaaa tagtcattgg aggggatgga gggaaggaac tggttgagag aggggctggg 140220 gagggaaatg gggagttggt catcaggtgt agggagagaa ggggaaaggg ggctgtgaga 140280 gtgaatggaa atttgtggca ggtgggagtg gggtgggggt ggggaggctg ccatatcatt 140340 tctaggatgt gtcagagacc tgggacaggg gaggctccag gtagactatg aggggcctct 140400 agctgagatt ccttgtaatg ggggatatgg agtctgaaat ggtcacttcc tgtggccaga 140460 caggacctcc cagtagaggg aaaaggatac caacccaccc acaaaacctt tgaccctaaa 140520 tttgtcctgc ctacaagaag ttccgggaca aagatggaac agagacaaag ggaacgacca 140580 accaatgacc agcccaactt gagacccgtg ccgtgggcag gaactaatcc ttaagattta 140640 atgatactct tgttatgctt gcagacaaga gcctggcata tctgtcctct gagaggctcc 140700 acctagcagc tgactgaaac agatgcagag actcatagcc aaacattaga cggaacttgg 140760 aaaatgtgga agagttgagg gaaagattga gggacacaga gaggatagga actccacacg 140820 aacaacagag tcaactaacc tggactcttg agagcttcca gagactgaac caccaaccga 140880 gcatgggctg gaccttggca tcctgcattt atgtagcaga tatgcacctt ggtcttcatg 140940 agagtccctc aacaactaga gcagggactg tccttgaccc tgttaactgc ctgcctgtgg 141000 atcctattcc aactgggctg tcttgtctgg cctcagtgga agaggatgtg cctagtcctg 141060 caatgatttg atgtgccagg gtgggttggt acccagcacc cccctcaaag gagaagagga 141120 tgagggagga gctatgtgat gggggtactg ggaagagagg agagatgcaa attggaagta 141180 aagtgaataa ataaataaat taattaatgg aaaaaagtag tgggtgctat gaaggagtca 141240 tctatattag attttctgga gttataacaa aatacttgag agaaatggag ttattttatt 141300 atcgttttgg agataaaagt ccaaataaca gtagtattat ctgtggtaag aactactgta 141360 gatggcactg tgataagaat ttgtcagttc ctatgtctgt aaatgtgtag cgtgtgtgtg 141420 tgtgtgtgtg tgtgtgtgtg tgtgtctgca tgtgcatgta tgtggggtgt ctatgtttga 141480 gatggatatg gatgtgctgt gtgttatatg tatgtgtgat atatgtgctg tgtatgtgga 141540 agttatgtgt gggtatgggt gggggctatg tgtgaaagaa agagagaaac aggataggga 141600 aaggaagtca gagccagacc tatatgggga cagtaatgac ctccttattt agatttatct 141660 cttaagggtt aaagatgaca ttatttttta acattgttgc tccaggacca ggctcctaat 141720 gcatgcagtc tgagagacac attcaaatca tatcctatac acagtaccat cctttggcca 141780 gaatattggt aaaaccatcc aaatgttaga ggagagtagc caacagttct ggcatccgta 141840 tacagcactg ccggcgtaga gtgccctact gccctgaaga tgccctgtga tttcattgaa 141900 aattctttcc agattggtaa cctctcagcc cggctctctg tgccttatag atcatgagat 141960 ctgtaacata caacgtaaca attagcttct cagttcttgt tggagaggct gggtttataa 142020 gaaagaaagc agccaagcac atcagcccac gggagcctgg cataaagtgt acctcagcag 142080 ttccttagtg tttgtagaat aaagtaatgt attatagctt tcctatagat ccacatggaa 142140 gcctgagaag ctatgtggtt ctcctcagac ctcatagtct gctcagattc actcacttgg 142200 gcgggttgta tagctctggg gaagatttca cttctctgcc tcagcaacac aggctcaggc 142260 gcatcctgct ttgggttgtg aagttgaccg gaagtgacac gtatagcttt cagaaagaag 142320 tttgaagagc cagcatgtga ctgaccattc gtgcccttat accttgtttt ttccaaggat 142380 tatgatagag ccacaggcca ttgggtgctg atatttgttt tcatggtgta actaaatctg 142440 tccgcagagc aacacttctt ttttatgaag ataagaagta actgaggcag gtttccaatc 142500 tagacatggt tactgtaggg tctgctactt gctagcttca cccagctgaa tcaagaagag 142560 aaaacaactg gccaagcttg ttgggggcat acatgaaagt ctagtactta ggaggtggag 142620 gcaggaagat aaggagtgga aggtcagtct tgggtgcatt tgtgtgtgtg tgtgtgtgtg 142680 tgtgtgtgag agagagagag agagagagag agagacagag acagagacag agacagagac 142740 acagagagag acagagagag acacagagag agagagacag agagacacag agagagagag 142800 acagagagag agacacacac acacacacac agagagagag agagagagag agagagagag 142860 agagagagag agagatattc cctggaatca ggctcccatt acaagcacta acaattccat 142920 ctgatgcctt gcaaattgtt tcagaggcct gggaaagata ggaaaatttt ctgcctattt 142980 ttagaaagcc aatgtaacat tgtaccaaaa tcttataaag atagcattga aaaaaaagaa 143040 aaccaacatc cgatctcatt gaaggagtaa caaatcctaa attattaaaa taataacagt 143100 tcagcaaagc attaaaatat accaagtgaa gtcccctttt gacaatcaac aacggcacct 143160 taccccacac acattatgac tgactgtgta cccctctaaa ataggacgaa gggggaaaga 143220 atgcattgct tcagaaaatg acccagcaac atatgctagt tatctattta tttttcttgc 143280 cttgacagaa gctagctagt attatctgga agcagggaac aatgtctgtt gagaacctgc 143340 ctctgtccgg attgaactgt cacaaggcaa gtctgtgaag gcattttctt ggtttaataa 143400 ttgatgtggt aggtccagcc ctctgtaggt ggtaccacac tgagtgtgtg gtcctgggtc 143460 gcagggttaa ggaacctgaa catgagcctg gagaggaagc caggaagcag cactccttca 143520 tggcctctgc tccacttcct gctttgagtt tctagcttga attctcttca tgaaaaatta 143580 ttggctgggc tggcgagatg gctcagtggg tgagaacact gactgctctt ccaaaggtcc 143640 tgagttcaaa tcccagcaac cacatggcgg ctcacaatca tatgtaatga gattggacac 143700 gctcttctgg tgtgtctgaa gacagcagca gcatacttat ttatactaat aaataagtct 143760 ttgggccgga gcgagcagag gtcctaaatt caattctcaa caccagatga aggcttacaa 143820 ctaactatct gtacagctac agtgtgtaat catatacata aaataaataa ataaatcttt 143880 taaaaattga aaaattattt ggtgtaaggt aaagaaacaa aaacccaaac accctttcct 143940 tttcaagtcg cttttagtcg tggtgtttat cacaggagct ggaggccatg taagatgcgt 144000 aggatgtagc atttagaagc catgtaggac acctcgttta gtggagctaa gccactagtg 144060 tgatataaga gtccgacgtt aatagacggc tttaccaagg aataatgaat tctgtatttc 144120 aaacggagtc gggattggct tagggttaaa aagaacctag aagtcaaggt tccccaggct 144180 ggggtggctc tgatagacga gtgccaagca cagctgcaga tagcgcaaaa gtatgactta 144240 aaagatacat gctataagga agaaaagtga gggaaaattg caaaatatga aaatagcaag 144300 aaatttcatg tatgtatcaa gtttcccaaa caagggacag gcacacagtc acacagacac 144360 atagacacat agacacacag gcacacagac acacagacag gataggatat taagcactgc 144420 attttgttcc tcttgttgaa accctatgta gtttcattat gtaatccagg ctgtcctccg 144480 actcatgcta ctccggactc agtttcccaa gtgctggggt ttcaggcaca ctgctacctt 144540 gtcccactga aagagttgtg cctgagatgg tcattactta gtgaaggaca gtgagacttc 144600 agcaatggag atggcatcct tgttagtgga tttgtccact taaatgcatt agacaaagtc 144660 tggcttatca tttattatta ttgttattat tattattatt attattatta ttattattat 144720 tattttaagc atagagttag tgattactca ttcctatact ttcctattgt taaggaaaaa 144780 aatctaaaat ggcaaaatca caatgtgaaa tacatatgca aagatcttaa aaccaccagt 144840 ccgtgagtaa accagttcct tttggagaca aaggaatagg cagtaaataa gagaaaacca 144900 catttgttaa tggatgggta gttgttcact ctagccatgg agacactgaa tcagcactcg 144960 acacgattcc tttacatact tacagaggac attgattcgt ttctattgtt actgagtagc 145020 tccaacttcc caggtcacaa gttgccaaga gcagccaatt attcttaagt ttcaatttgt 145080 tgaaaaaaaa aaaagccctg atagcattga tgttcatccc cgagcaactc agtaactcag 145140 tgtgtgtgtg tgtgtgtggg ggggggtgtg tgtgtgtggg agggtatgtc tgtctgtgtg 145200 tctgtgtgca tatgtggaca tacatatgcc atgtcacata tgtggaaatc agaggacaac 145260 ctgcaggagt tagttctgtt cttgaaccat gtggggttct gggaattcga gtcaagccat 145320 taggtttggt gtcaagagct ttttcccgtt gagccatttt agcccataga aactggttct 145380 accacaaaca tttacaagat acaaaagcta aggacactgc ttagtccatt taaaatgata 145440 taggtcccaa agcttcgctt actgtgggat tttctcctat catctcatcc ctttctttca 145500 tctggtcttt aggatccttt tctgaaggtg tgaagttggt agagaagcct actgtgtgga 145560 caggaatgtc tctcaaatgc atcaccattt tgagtagtag tgattgagaa caaagggaat 145620 tgttgtaagg tgtggagtta tttgataagg tgtggttgta acaaagcacc aaaactgggc 145680 cagggagata gcttatcagg aaaagcactt ggtacagcct cctgaatgcc agagttggat 145740 ccctggagtc cacagaaaaa gccgagtgtg gaaagccaga tgcagtgaca ggaatttgta 145800 acttgggcat tcctgggaag agatgaaagg agaagatggg agaatggcct gaagcttaag 145860 ggacagctat gggatgtgtg ctacaagaac agaaactagg gagactctgc ctcaaagtgg 145920 ggtgggaacc aactacacac aggtatgttc aagcagacac cacacacaca cacacacaca 145980 cacacacaca cacacacaca cacacacaca cacacacaca ttattaaagc tgataacagc 146040 ttttagacta agtagaagga aaaagagata actgtagtta acaaaatcta attcccaatt 146100 tgctacctac attccctacc tgcagtatgc taactttaag agggaaaaag tcctccaacc 146160 agagtgtata taaaatatgt tgtctgatcc tcccaatagt ggttttctaa aaggaatatc 146220 atagaagata tttagaagac ttccttttac gttacttttt gagacagggt tttatgtaag 146280 ccaagttgac ctcaaacttt gaatcccact tctctacttc ctgagagcta cagctataaa 146340 tatgcaccat catggctggt ttacatggtg ctggggacag aacccaggac tttgtgatgc 146400 taggcaagtt ctctaccaac tgaggtcgat ccccagccct cagataacat tgtgtggcgc 146460 tattctgtct acccaagaga gtttatagtt tagacttcca gatcaagcga aataaagagt 146520 tctggggaga tgtccttttg cttcctagtg taggtataga tgttacggga tggcaggatt 146580 tagtcagagg aagagactca acttctctga aggtcttact atgcatacaa ctttctgcta 146640 tggttcttat cctgtggcat aaggacatcc cctgccaccc aattttacat gccacatccc 146700 aaatcattgt aacgaggctt cacatggcaa ccagcagttg actgcaatga gacttccatg 146760 gcaaccagca gttgactgta acaaggcttc catggcaacc agtagttgac tgcaacaagg 146820 cttccatggc aaccagcagt tgacacctgc cttgctctgg ttttgttaca gctgaactgt 146880 ttctcatctg tgaaacccct ttttaaaaca tgtattcatt tatcttatat gtgtacactg 146940 tagttatctt cagacacacc agaagaggac atcagatccc attacagatg gctgtgagcc 147000 accatgtggt tgctgggaat tgaactcaga acctctggaa gagcagccag tgctcttaac 147060 tgttgagtca tctctctggc acccccgctc ccatgaaacc cttttgcata tatctttttg 147120 tcttctagtc ttgtggtcca caagccctac aagtcacttt tggagttctt ccttagcccc 147180 actcccacat tcttgctttg gattatcttc gacattcagt gctctcaggg cagctgtgaa 147240 cctccttgaa aaattttctc tcttgtctgg tctctaatga tgtgtagact tgacttacta 147300 ggggttcatg tcttgctctc tctttcatgc agttcaattc atatttagca tcctaagtca 147360 gtgactatag ggaaaggttt tgagccccct tggaagtctc tgctgcaagc atagatcttg 147420 cagagtagat atttaatgaa ctcttattaa atccatgcat gaatgaaact cttcatgtgt 147480 atttcccaat cttctctcta catcattgaa aggggaataa cagaatttaa attcttcaca 147540 attcctgcaa agggagactt ctactaagta tgaaatgtgc aaggcttgaa tttgtaatga 147600 atgtctgatg taacccagca ttcccagaat caccacttgg gttttccaca agggtatgca 147660 ttcctctttt atacaaatgc atggaggggg aggggttaca ggataggcag aaatgaaaga 147720 tatttgttaa ttttctgtta ttgtgataaa acactatgat caaggaaact aatcgaagaa 147780 aaaagtttat gtggggttat ggtggccgag gattagagtc catagcttgc aatggaggtt 147840 gcaggtaacc agaacagaac aggctcacat ccctgtcttc aagcaggaaa cagaaagcat 147900 gcggagtggt gtaaggcatt aaacctcaaa ggccatccca caggtcacac ttcctatacc 147960 tccccaaaca gtgccaccaa ttgggaacca aacatcagag tctgtggagg acatattcgt 148020 tcaaaccacc accacagaca agtagtttat tctgtgcacc cttcttatgg aggaggctga 148080 caagtaccac tgtttacaca cagtaggact gccacgaaca atatagccat actcactacc 148140 tgctttcccg gtttgtggcc ttcccatacc tcacagaact ttcttcttcc tgcctgtaca 148200 tttcactgct gtctcttgct gtggagtggg gctctatatg tgcgagggtc tcagtgtgaa 148260 gcaggctctg tgctacttgt cttatcctga gttacagaga ttattttttg tcccagtccc 148320 tatcaggagt cactattttg agcccaatct ggtagctctg gtttcttctt tccagaatat 148380 tctgagtcgc gcagcctcaa accggctctg cctttcctct acatgcctgt gatgacgact 148440 gcctggctgc agatgctccg ggtgttttcc cacaccagtt tctcttgttg tgactccatc 148500 aaacccaggt cagcttctgt aatcgagacg atccacttaa ttaactgtaa tgaggctccc 148560 atcatttcca ataaatttct ttccaaaaca ccacttgaga ggcacatagc ttcctatcag 148620 ctgtaaattt tttttttcct gtctatttag ttccaggaat tgaatttact cagagatgtc 148680 attgcccaac ctcatgtctc aatgttttaa actaaatctt tcagtctggt gctatgggct 148740 acaggaccca accccagttt cccttgtaca gaatggaaga tgccgtgcaa acagcttgtc 148800 tgggtttcac cagagctggg agaatcagtg ctggttttca tggcctcttc tgcgatcagc 148860 cccttctcca ccaacagttt tgttagttag acagagcaac ttgcgcttga agatctcaaa 148920 ccatttatcg aagcatgcca tcttctcctt tttaaagacc tgaaatgctg ttggaataat 148980 tttgctgtgt tttataaaaa tatagagagg attaatacgt ttggtagagg tgcaataagg 149040 tgtggaggga aggggtgcct ccaaaggcct gtgctgaggc acctcttccc cctgagcgac 149100 cagccaaaga atggtatagt atagaataga gtttattcat ggcatgggga ggggagttga 149160 gagagtagta gagacagaga aagagagagg gagagagaga aagagagaga gagggggggg 149220 agggagggag gaagggagag agagagagag agagagaggg agggagagag agagagaaga 149280 gtagtagagg ctggtcatga gcatgtggag agagaggggg aggggaatgg ggaatggggg 149340 agcaggaggg agaggacaga gtcggagcaa gaaagcaaga gagaacagag gggctaagca 149400 gtcccccttt tttcatttta aaaattttat tagatatttt ctttttttac atttcaaatg 149460 cttcctatac cctcccctcg ccctgctccc ctacccaccc atagcagccc cttttatagt 149520 gagtcaggca cacctggctg ttgccaggta actggggcag agcctataca aaaatgctaa 149580 cagatttgtc agtgaaatga tattttgctc tttgacttat agaagatgaa aaagaaaacc 149640 acaagtttca tactgaccct ttggggagga tacatcttta agaaattatg ggagagaata 149700 gtttcattta gaaagaattt aagtgttttt ttttcccctc tcagacatgt catggtttaa 149760 tatcagttat ccaatttttc tttgtgctac tctcagaatt cttcctgaga aattagcttt 149820 aatcttctgc tttgtttcaa agcctgcact agattaagcc tcgtcctctc tctctctcct 149880 ctctctttct ctttggcttc attcgggaat gactaattag ccaggagaaa ttaaatttac 149940 aaattaaacc tctctgcata gattttctca agaccccagc cctgggggta atatccatgg 150000 cctctcctac agagcttttg cctcccagag ggttgtatag aggggtgaaa tgtttatgaa 150060 gccacatcgg cttgaagagc aggttggtgg ttggcggttg gcagcattat aagttgttct 150120 ttggttcctt cagggaaaaa cttcagtgtt ccaaagttca gggtcatgga aggagggtga 150180 ggaatgttcc cagccagaga agaaagtgat gtgtgtgaga gagaactgcc gagaactgta 150240 gaactgaagt ttccaccgct ccaattacat gcctgaatgc cttcttttca tttcatctct 150300 gtttattatc tttgtctgtg tgtggatggt gtatatatgg tatgtgcatt tatgtgtgtg 150360 agtgtacact tatgcatata aattcaagtg ctcatgtttt actccacgta taaagagatc 150420 agaggacagc cttgggtatt gttcattgcc tttcaccttt ctctttctct ctcttgaata 150480 caccacctat cattgtagga gactggcatc acagacctgc catggctggc tttgtatggg 150540 atgcagggat gtgaatccag gtcctgatgt ctatgtggca tagacactta actaggaccc 150600 caacccattc acctcccaag cctgactctg ctcccctgct ccactctaca acatgccctc 150660 tactcgataa aggcactaaa atgtgtgact aggggccatc cagtttgttc tgagtgtcag 150720 ctcatgcctc cttcttagga agcatttcaa gccaatgtct cttaatacca cccacggttc 150780 attgttttct ggcttctcct tccttccttc cttccttcct tccttccttc cttccttcct 150840 tccttccttc cttcctccct tcctcccttc ctcccttcct cccttcctcc cttcctccct 150900 tcctcccttt ctcccttcct tgctttttct ttctaacatc ttttgtgtat tgaaaatgct 150960 gatgtttttg ctatttgggg atgagctctt gcaggatagt gttccccaac tcttctcttc 151020 caagtttatt ctccagatct ttgcattaag ttaggcaggc tcctttaggg acagagaccg 151080 acggcttact ggaggggaag gcttcagtcc cattaaaaga tggttaatga acattcaaag 151140 ttagtgtttc tctttgtttc agctgcagat cttttggaga atcttaggag aaagcagcca 151200 agaccactga atccacctga cctcattaac ctggagcaaa ctgggcttat tagaatttag 151260 attgaaggcc tcagaggtta gatattgctc ttggagaaga cctaggttca gttcctagca 151320 cccacatagg tcggattaca attgctgata actccagttc caggggatct gtcactttct 151380 tctgaccttc caggtcttca tgcacattgt gcacatacag acaagcacac gcacacacac 151440 acacacacac acacacacac acacacacac acacaaatat taaaactctg gagtgtgaac 151500 ttagtatgcc cataggtaat tatgtccatt agctttcctc attggacatt ttctctgtca 151560 cctttaactc aggatgctca caaatatccc tacccctcta cgtacttcct ttatgaattt 151620 gtatatgccc cttcccttgt ttggggagca gatgtttctc tactctttga tctgactgtt 151680 gtgctcactg gtttcatact caccaagaga cctgattcga cttaggtacc gagtcaccca 151740 cgtctctcag ggtcagcctg ctctgtctct cagtttttgg tctaagaaaa caaggccttc 151800 cacccttttg atcagcagat atgagtaaaa gaaataaaag ttgatgctct ttgttttcca 151860 gggattcaga taaaatgttt gccatatgca tgaaataatt tttaagaggt aggttggttc 151920 ttccccctaa agcaaggttt atctaagaag agtgctttta aggatggtcg tgatgctgac 151980 agtacctcgc attcctaccg tcatttctgg aaaagccatt ttaatttttc atctcacctc 152040 gccatctggg agacaagtga actcaatgtt ttccttgcca tttataaagt gagttagtta 152100 agactcgggt aagactattg atctgcacca agatccaata ccaggaaaag agatgaaatt 152160 ggtatccaaa cttttgtttc aggactctga gccctggcct ctattgtctc aaacacagtt 152220 caatcataaa ctgagtcaat acatctagca ctgcctggca gtataaggca attaaactat 152280 cacaaggcta tgggggtaaa gacactatca caaaggtctg ggataaataa acttgaacag 152340 gttgataaag tgtgggactc aatctctaaa ggaggtgctg taatctctct gtagtggaag 152400 gtttacctga aggaacagat ttgaagttat tcctgaaagc atactaaaac cagaagacag 152460 tttggcagtc cgaatcaaaa actttaacta aagacatgaa ccacagtact ttccagcata 152520 tcactcagtg gctctaaagt tcttttcctt gtttgaaaac aatagagtcc aggggaggat 152580 taaatggtga tttgtaaagt gccaggctca gggcacattc agtaaatgtt accagtgatt 152640 gcagaataat ggtgataata ttttcctttg taggaggaaa taatgagcac gtagatttag 152700 caacacacag atcgtttgtt gtgcttttaa aagtcacagt gaaaagttgg aaacaacttg 152760 atgtccagca agagggaatt gcagaagaga gtttcctggt atttctaggt gctagatcat 152820 tagatagcca tgtaaaagaa taaaaggaaa taaatatgtt attgcctagc aagatggcca 152880 tgaatttgtt gttgttgttg ttattaagac agggtttctc tctgtagccc tggctgacct 152940 agatctatag accaggctgg cctcaaactc aaagatcgat ctgcatctgc ctcctgagtg 153000 ctggggttaa aggcttccac cactgctgcc tggctataaa tgtttaaaat atattttcta 153060 ttaattcttc acaaattcca tatatgtgta cagtgtattt tgaccatatt cacccctact 153120 cctcttccca acccctttca gctcccctgc tgccatattt ccatccctta ccaattttgt 153180 gtactattta attaatagat atatacatat atatatttaa ttctgatata tcagcacatt 153240 atatattata ttatattata tgttacatat attatctgtt acataatata catgtataca 153300 tgtatgtata tatatatata tatatatata tatatatata tatatatata tatacatatt 153360 gatgtacacc cattcattgg agtctggatg ccttgccagg gaccactctt aagcaaagtg 153420 gcctctccct gatctggaag tcccttgact gttaacagat ccttagctag ggctggagac 153480 ttgggagccc catgcctctc taagcaggaa tcctgacttg gtcttgggct gccactgtga 153540 gctcatgagg cagtgttggg ggagggatgc tttataccat cccctttgtg actgagaatt 153600 ccacaaatgc tcattctctg cacttggcag tgatttttta cctttcaaaa acacttataa 153660 aatattaaat cctttttttt taagtttgtg cttttttgat gttgtttaac acatatattc 153720 atgtacagat acaggatagc tttaaaaact aggagatgaa tttgcagctc tatgtcaaca 153780 aaactgacat ttaaaaattg tttttattaa aattcttttt atttaaacta tcccagtaac 153840 aaaggcaagt atcatgtaaa ataaaattgt gctgttttgt tttgtttctg gctttcttgt 153900 ttcctactct ttcctttggt ttgtcagctc tgtgctacca taagcagaca cgatccattc 153960 aatagttgac taacagcatt cttttgagtg tttactgtgg ccaaggccct ggttctaagc 154020 acttgatgta cagataaacc agatgaacaa caatgccctt caaaagctta aattcaaggc 154080 tggagaaatg gctcagtggt taagagcact gtctgctctt ccagaggacc caggtttgac 154140 tcccagctcc cacatggcag cttataacca tctacagctc cagtcctggg aggtctgaca 154200 ctctggtacc cacgtggtac atataaatat atgcaagcga gacatgtgca catataattg 154260 aaatataggt atagtgatct tgggattcca gtgtctgaag caggagaagg atgagactga 154320 gatcagcctg gaataagatc ctcttttgat taaaacaaaa gcatgtacta ggtgacatga 154380 acagcacaac cgcaagagaa ggagagtaga ccaaaatttc tagaggagct atgccttctt 154440 aacagcattc aacccctctt ctgagaatcc ctgcagactg ccctccagca tcactgattt 154500 ccatagaggc tgcatagctg tcaaagttca cagtctactc agaggtaacc tgctgtgtca 154560 atatttgtaa tttgtacttc atggtgagtc acttccagag aatagattat ctaccatgga 154620 agggaaaagg tctgggagtg aaagtgagct ggacaagaca caggtggagg cattaggttt 154680 caacagcttc ctgcctctac aaagcaaagg gcaggaatgt gtgctggcgt gggctggagc 154740 ccagaacagc cacaactctc aagtggagga taagcagaaa gcagggttag tctcctgttt 154800 ctccaggtgg ttctagctga ttcttctagg tctggaaaca aatgttacct tctatccatt 154860 ttctctcctt ctcccctggc ttccattttt atagctcaga agcaaatcca tcttttaaga 154920 gttgttctca agcaatcttt gtctaaagct ggatcctcac catactacat cccacttcac 154980 ccccacctcc accctcactg ccaattgagg aagttctctg aattctgaca cctccatagc 155040 atgtgatagt cccctatgtt attcctatgc atcaattcat ttttacattg tgctggagaa 155100 ctccatctct cttacaagag tagatgatca ttaaggtttg gtctatagaa ctccaaatag 155160 actggactag aaaagaaatt ccttctgaca cataataatc agaacaacaa atgcactaaa 155220 taaagacaga atattgaaag cagtaaggga aaaaggtcaa gtaacatata aaggcaggcc 155280 tattagaatt acaccagact tctcaccaga gactatgaaa gccagaagat cctggacaga 155340 tgttatgcag accctaagag aacccaggcc aggctactat acccagccaa actctcaatt 155400 accatagatg gagaaaccaa agtattccat gataaagcca aattcacaca atatctttcc 155460 acaaatcgag cccttcaaag gataataaag ggaaaacacc aacacaagga cagaaactac 155520 accctagaaa aagcaagaaa ataatctttc aacaaaccta aaagaagact gcagcaagaa 155580 cagaatccaa actttaacaa caaaattaac aggaagcaac aattactttt ctttgatttc 155640 tcttaacacc agtggactca attgcccaat gaaaagacat agactaatag actggctaca 155700 caaacaggac ccaacatttt gctgcttaca ggaaacccac ctcagggaaa aagacagaca 155760 ctacctcaga gtgaaaggct ggaaaacaat tttccaagca aatggtctga agaaacaagc 155820 tggagtagcc attctaatat cgaataaaat cgacttccaa cccaaagtta tcaaaaaaga 155880 caaggagggg cacttcatac tcatcaaata taaaatctac taaggtaaat tctcaattct 155940 taatatctat gctccaaata taagggcagc cacattcatt aaagaaactt tagtaaagct 156000 caaagcacac attgcacctc acacaataat agtgggagac ttcaacacct cactctcacc 156060 aatggacaga tcctggaaac agaaactaaa cagagacaca tggacactaa cagaagttat 156120 aaaacaaatg gatctaatag atatctacag aacatcttat cctaaaaaaa aggatatacc 156180 ttcttctcag cacctcatgg taccttctcc aaaattgacc atataattgg tcacaaaaca 156240 ggcctccata gatacaaaaa tattgaaatt atctcatgca tcctacctga tcaccacgga 156300 ctaaggctga tcttcaataa cagcataaat aatagaaagc caacattcac gtggaaactg 156360 aacaagactc tactcaatga ttccttggtc atagatgaaa taaagaaaga aattaaaaac 156420 tttttagagt ttaatgaaaa tgaagccaca acatacccaa acttacggga cacaatgaag 156480 gcagtcctaa gaggaaaact catagcccca agtgcctcca aaaagaaact agagagagca 156540 tacactagca gcctgcagca cacctagaag ctctagaact aaaggaagca aattcaccca 156600 agaggagtag atggcaggaa ataatcaaac tcagggctga aatcaaccaa gtggaaacaa 156660 aaagaactat tcaaagaatc aaccaaacca gaagctggtt ctttaagaaa atcaacaaga 156720 tagataaacc cttagccagt ctaactagag ggcacttgta tccagtctaa ctagagggac 156780 agtatcctac ttaacaaaat caaaaatgaa aaaggagaca acaacagatc ctgaggaaat 156840 ccaaaacatc atcagatcct accacaaaag tctatactca acaaaactag aaaacctgga 156900 tgaaatggac atcttcctag gcagatacca ggtaccaaag ttaaatcagg attagattaa 156960 cgatctaaac agtcccattt ctcctaaaga aatagaagca gtcattaata ggctcccaac 157020 caaaaaaaaa aaaaaaaaaa accaggacca gatgggttta gtgcagagtt ctatcagacc 157080 ttcaaagaag atctaattcc aactctcctc aaactattcc acaaaataga aacagaaggt 157140 actctaccaa attcattcta tgaagccaca attactctta tacctaaacc acacaaagat 157200 ccaacaaaga aagagagctt cagaccaact tcccttatga atatccatgc aaaaatactc 157260 aataaaatcc ttgcaaaccg aatctaagaa catatcacaa tgatcatcca tcatgatcaa 157320 gtaggcttca tcccagggat gcagggatgg cttaatatac ggaaatccat caacataatc 157380 cactatataa gcaaactcaa agacaaaaac cacatgatca tcttgttaga tgctgagaaa 157440 gcatttggca aaatccaaca ccccttcatg ataaaagtct tagaaagatc aggaatttga 157500 ggcccatacc taaacataat aaaagcaata tacagcaaac cagtagccaa cctcaaacta 157560 aatggggaga aactcaaagc aatcccacta aaatcaggga ctagacaagg ctgcccactt 157620 tctccgtact tattcaatat agtacttgaa gtcctagcca gagcaattag acaacaaaag 157680 gagatcaagg ggatacaatt tggaaaggaa gaagtcaaaa taatcactat ttgcagatga 157740 tatgatagtg tatatataag tgatcttata aattccacca gagaactcct aaacctgata 157800 aacaccttca gtgcagtagc tggatataaa attaactcaa acaaatcaat ggcctttctc 157860 tacacaaagg ataaaagggc tgagaaagaa attagggcaa caacacccat cacaataggc 157920 acaaataata taaaatacct tggtgtgact ctaactaagg aagtgaaata tctgtatgat 157980 aagaacttca agtctctgaa gaaataaatt gaagaagatc tgagaggatg gaaagatctt 158040 ccatgcagga ttaattggca ggattaatat agtcaaaatg gctattctgc caaaagcaat 158100 ctatagattc aatgcaatcc ccataaaaat tccaacataa ttcttcactt agttagaaag 158160 ggcaatttgc aaattcatct ggaataataa aaaacatagg atagtaaaat ctattctcaa 158220 caataaaaca acctctggtg gaatcaccat acctgacctt aagctgtact acagagcaat 158280 tgtgatgaaa actgcctggt actggtacaa tgacagatag gtagatcaat ggaatagaat 158340 tgaaggccca gcaatgaatc cacacagctg tggtcactgg atctttgaca aaggagctaa 158400 aaccatccag tggaaaaaag gcagcatttt caacaaatgg tgctggcaca attggcggtt 158460 accatgtaga agaatgggaa ttgatccatt cttttttttt ttttttttcc attttttatt 158520 aggtatttaa ctcatttaca tttccaatgc tataccaaaa gtcccccata tccacccacc 158580 cccactcccc tgcccaccca ctcccccttt ttggccctgg tattcccctg tactggggca 158640 tataaagttt gcaagtccaa tgggcctctc tttccagtga tggccgacta ggccatcttt 158700 tgatatatat gcagctagag tcaagagctc cggggtactg gttagctcat aatgttgttc 158760 cacctatagg gttgcagatc cctttagctc cttggctact ttctctagct cctccattgg 158820 gagccctatg atccatccat tagctgactg tgagcatcca cttctgtgtt tgctgggccc 158880 cggcatagtc tcacaagaga cagctacatc tgcgtccttt caataaaatc ttgctagtgt 158940 atgcaatggt gtcggcgttt ggatgctgat tatggggtgg atccctggct atggcagtct 159000 ctacatggtc catcctttca tctcagctcc aaactccgtc tctgtaactc cttccatggg 159060 tgttttgttc ccaaatctaa ggaggggcat agtgtccaca cttcagtctt cattctcctt 159120 gagtttcatg tgtttagcaa attatatctt atatcttggg tatcctaggt ttggggctaa 159180 tatccactta tcagtgaata catattgtgt gagtttcttt gtgaatgtgt tacctcactc 159240 aggatgatgc cctctaggtc catccatttg gctaggaatt tcataaattc attcttttta 159300 atagctgagt agtactccat tgtgtagatg taccacattt tctgtatcca ttcctctgtt 159360 gaggggcatc taggttcttt ccagcttctg gctattataa ataaggctgc tatgaacata 159420 gtggagcatg tgtccttctt acctgttggg gcatcttctg gatatatgcc caggagaggt 159480 attgctggat cctccggtag tactatgtcc agttttctga ggaaccgcca gactgatttc 159540 cagagtggtt gtacaagcct gcactcccac caacaatgga ggagtgttcc tctttctcca 159600 catcctcgcc agcatctgct gtcacctgaa tttttgatct tagccattct gactggtgtg 159660 aggtggaatc tcagggttgt tttgatttgc atttccctga tgattaagga tgttgaacat 159720 tttttcaagt gcttctctgc cattcggtat tcctcaggtg agaattcttt gttcagttct 159780 gagccccatt ttttaatggg gttatttgat tttctgaagt ccaccttctt gagttcttta 159840 tatatgttgg atattagtcc cctatctgat ttaggatagg taaagatcct ttcccaatct 159900 gttggtggtc tttttgtctt attgacggtg tcttttgcct tgcagaaact ttggagtttc 159960 attaggtccc atttgtcgat tttcgatctt acagcacaag ccattgctgt tctgttcagg 160020 aatttttccc ctgtgcccat atcttcaagg cttttcccca ctttctcctc tataagtttc 160080 agtgtctctg gttttatgtg aagttccttg atccacttag atttgacctt agtacaagga 160140 gataagtatg gatcgattcg cattcttcta cacgataaca accagttgtg ccagcaccaa 160200 ttgttgaaaa tgctgtcttt cttccactgg atggttttag ctcccttgtc gaagatcaag 160260 tgaccatagg tgtgtgggtt catttctgga tcttcaattc tattccattg gtctacttgt 160320 ctgtctctat accagtacca tgcagttttt atcacagttg ctctgtagta aagctttagg 160380 tctggcatgg tgattccgcc agaagttctt ttatccttga gaagactttt tgctatccta 160440 ggttttttgt tattccagac aaatttgcaa attgctcctt ccaattcgtt gaagaattga 160500 gttggaattt tgatggggat tgcattgaat ctgtagattg cttttggcaa gatagccatt 160560 tttacaatgt tgatcctgcc aatccatgag catgggagat ctttccatct tctgagatct 160620 tctttaattt ctttcttcag agatttgaag tttttatcat acagatcttt cacctcctta 160680 gttagagtca cgccaagata ttttatatta tttgtgacta ttgagaaggg tgttgtttcc 160740 ctaatttctt tctcagcctg tttattcttt gtatagagaa aggccattga cttgtttgag 160800 tttattttat atccagctac ttcaccgaag ctgtttatca ggtttaggag ttctctggta 160860 gaatttttag ggtcacttat atatactatc atatcatctg caaaaagtga tattttgact 160920 tcctcttttc caatttgtat ccccttgatc tccttttgtt gtcgaattgc tctggctaat 160980 acttcaagta ctatgttgaa aaggtaggga gagagtgggc agccttgtct agtccctgat 161040 tttagtggga ttgcttccag cttctctcca tttactttga tgttggctac tggtttgctg 161100 tagattgctt ttatcatgtt taggtatggg ccttgaattc ctgatctttc caaaactttt 161160 atcatgaatg ggtgttggat cttgtcaaat gctttttctg catctaacga gatgatcatg 161220 tggtttttgt ctttgagttt gtttatataa tggattacat tgatggattt tcgtatatta 161280 aaccatccct gcatccctgg aataaaacct acttggtcag gatggatgat tgctttaatg 161340 tgttcttgga ttcggttagc gagaatttta ttgaggattt ttgcatcgat attcataaga 161400 gaaattggtc tgaagttctc tatctttgtt ggatctttct gtggtttagg tatcagagta 161460 atagtggctt cataaaatga gttgggtaga gtaccttcta cttctatttt gtgaaatagt 161520 ttgtgcagaa ttggaattag atcttctttg aaggtctgat agaactctgc actaaaccca 161580 tctggtcctg ggcttttttt ggttgggaga ctattaataa ctgcttctat ttctttaggt 161640 gatatgggac tgtttagatg gtcaacttga tcctgattca actttggtac ctggtatctg 161700 tccagaaatt tgtccatttc gtccaggttt tccagttttg ttgagtatag ccttttgtag 161760 aaggatctga tggtgttttg gatttcttca ggatctgttg ttatgtctcc cttttcattt 161820 ctgattttgt taattaggat tttgtccctg tgccctttag tgagtctagc taagggttta 161880 tctatcttgt tgattttctc aaagaaccaa ctcctcgttt ggttaattct ttgaatactt 161940 cttcttgttt ccacttggtt gatttcaccc ctgagtttga ttatttcctg ccgtctactc 162000 ctcttgggta aatttgcttc ctttttttct agggctttta gatgtgttgt caaggtgcta 162060 gtatgtgctg tctctcgttt cttcttggag gcactcagcg ctatgagttt ccctcttaga 162120 aatgctttca ttgtgtccca taggtttggg tacgttgtgg cttcattttc attaaactct 162180 aaaaagtctt taatttcttt ctttattcct tccttgacca aggtatcatt gagaagagtg 162240 ttattcagtt tccacgtgaa tgttggcttt ccattattta tgttgttatt gaagatcagt 162300 cttaggccat ggtggtctga taggatacat gggacaattt caatattttt gtatctattg 162360 aggcctgttt tgtgaccaat tatatggtca attttggaga aggtcccgtg aggtgctgag 162420 aagaaggtat atccttttgt tttaggataa aatgttctgt agatatctgt caggtccatt 162480 tgtttcataa cttctgttag tttcactgtg tccctgttta gtttctgttt ccacgatctg 162540 tcctttgaag aaagtggtgt gttgaagtct cccactatta ttgtgtgagg tgcaatgtat 162600 gctttgagct ttactaaagt gtctctaatg aatgtggctg cccttgcatt tggtgcgtag 162660 atattcagaa ttgagagttc ctcttggagg attttacctt tgatgagtat gaagtgtccc 162720 tccttgtctt ttttgataac tttgggttgg aagtcgattt tatccgatat taaaatggct 162780 actccagctt gtttcttcag tcgatttgct tggaaaattg ttttccagcc tttcactctg 162840 aggtagtgtc tgtctttttc cctgagatgg gtttcctgta agcagcagaa tgttgggtcc 162900 tgtttgtgta gccagtctgt tagtctatgt ctttttattg gggaattgag tccattgata 162960 ttaagagata ttaaggaaaa gtaattgttg ctccctttta tttttgttgt tagagttggc 163020 attctgttct tgtggctgtc ttctttttgg tttgttgaat gattactttc ttggttgttc 163080 tagggcgtga tttccgtcct tgtattgctt cttttctgtt attatccttt gaagggctgg 163140 attcgtggaa agatattgtg tgaatttggt tttgtcgtgg aatactttgg tttctccatc 163200 tatggtaatt gagagtttgg ccgggtatag tagcctgggc tggcatttgt gttctcttag 163260 tgtctgtata acatctgtcc aggctcttct ggctttcata gtctctggtg aaaagtctgg 163320 tgtaattctg ataggccttc ctttatatgt tacttgacct ttctccctta ctgcttttaa 163380 tattctatct ttatttagtg catttgttgt tctgattatt atgtgtcggg aggaatttct 163440 tttctggtcc agtctatttg gagttctgta tgcttcttgt atgatcatgg gcatctcttt 163500 ttttatgttt gggaagtttt cttctattat tttgttgaag atattagctg gccctttaag 163560 ttgaaaatct tcattctcat caattcctat tatccgtagg tttggtcttc tcattgtgtc 163620 ctggattacc tggatgtttt gagttaggat ccttttgcat tttgtatttt ctttgactgt 163680 tgtgtcgatg ttctctatgg aatcttctgc acctgagatt ctctcttcca tttcttgtat 163740 tctgttgctg atgctcgcat ctatggttcc agatctcttt cctagggttt ctatctccag 163800 cgttgcctcg ctttgggttt tctttattgt gtctacttcc ccttttagtt ctagtatggt 163860 tttgttcatt tccatcacct gtttggctgt gttttcctgc ttttctttaa gagcctgtaa 163920 ctctttagca gtgctctcct gtaaatcttt aagtgactta tgaaagtcct tcttgatgtc 163980 ctctatcatc atcatgagaa atgtttttaa atctgggtct agattttcgg ttgtgttggg 164040 gtgcccagga ctaggtgggg tgggagtgct gcgttctgat gatggtgagt ggtcttgatt 164100 tctgttagta ggattcttac gtttgccttt cgccatctgg taatctctga agctagctgt 164160 tttagttgtc actgttaaga gcttgttctt caggtgactc tgttagcctc tatgagcaga 164220 cctggagggt agcactctcc ttagtttcag tgggcagagt attctctgca ggcaagctct 164280 cttcttgcaa ggcaggtacc cagatatctg gtgttcgaac cagactcctg gcagaagttg 164340 tgttccactc actagaggtc ttaggatcac gtgtggaatc ctgtgtgggc ccttgcgggt 164400 gtcaggcgac tcagctggca aggtagccgg ggctcgagtg gagtggaagg ggtttgtgcc 164460 ccagatcaag cccgggtagc ctgcttccct atgtaccgca gtctcaagtt ccacgcgatt 164520 ggattggggt aggcgctgtg ttccactcac cagaggtctt agggtcccgt ggggagtccc 164580 gtgtggaccc ttgcgggtgt tgggcaagac tctgctggca aggtagcccg gggctcgagt 164640 ctcgagtcga gcagaaggga cttgtgcccc agatcaggcc cgggtagcct gcttccctat 164700 gtaccgcagt ctcaagttcc gcgcgattgg attggggcag gcactgtgat ccactcacca 164760 gaggtcttag ggtcccgtgg ggagtcccgt gtggaccctt gcgggtgttg ggcaagactc 164820 tgctggcaag gtagcccggg gctcgagtct cgagtcgagc ggaagggact tgtgccccag 164880 atcaggcccg ggtagcctgc ttccctatgt accgcagtct caagttccgc gcgattggat 164940 tggggcaggc actgtggtcc actcaccaga ggtcttaggg tcccgtgggg agtcccgtgt 165000 ggacccttgc gggtgttggg caagactctg ctggcaaggt agcccggggc tcgagtctcg 165060 agtcgagcgg cgaattgatc cattcttatc tccttgtaca aagctcaaaa gtaagtggat 165120 taaagacctc catataaaag cagagacact gaaatttata gaggagaaag tggggaaaag 165180 cctcgaagat atgggcacag gggaaaaatt cctaaacaga acagcaatgg cttgtgctgt 165240 aagatcaaga attggaaaat ggaacctcac aaaattgcat agcttctgta aggcaaaaga 165300 caccgtcaat aagacaaaaa ggccaccaac tgattgggaa aggattttta ccaatcctaa 165360 atctgatatg ggactaatat ccaatatata caaagagctc aagaagttag actcccgaaa 165420 ttcaaataat cccattaaaa acggggtaca gagctaaaca aagaattctc aagtgaggaa 165480 taccaaaggg ctgagaagca cctgaaaaaa tgttcaacat ccttaatcat cagggaaatg 165540 caaatcaaaa caaccttgag attccacctc acaccagtca gaatggctaa gatcaaaaat 165600 tcaggtgaca gtagatgctg gcgaggacgt ggagaaagag gaataatcct ccattgctgg 165660 taggattgca agcttgtaca accactctgg aagtcagtct tgtggtttct cagaaaattg 165720 gacatagtac tgctggaaga tccagcaata cctctcctgg gtatataccc agaagatgtt 165780 ccaactggta ataaggacac atgctccact attttcatag cagtgttatt tataatagcc 165840 agaagctgga aagaacccag atgtccctca acagaggaat ggataaagaa aatgtggtac 165900 atttattcaa tggagtacta ctcagctatt taaaacaatg aatttatgaa attcttgggc 165960 aaatggatgg atctggagga tatcatcctt agtgaggtaa cccaatcaca aaagatttca 166020 ccagatatgc actcagataa gtggatatta acctagaaac ttagaatacc caagatacaa 166080 cttccaaaac acaagaaaat caagaagaag gaagaccaat atgtggatac ttagaataag 166140 gaacaaaata gccatgaaag gagttataga gatgaagttt agagttaaga caaaagggtg 166200 gactatccag agactacccc atcttgggat ccatcagcca ctaaacccag atactattgc 166260 atatgccagc aagattttgc tgaagggacc ctgatatagc tgtctcatat gaggctctgc 166320 cagtgcctgg caaatacaga agtggatact cacagtcatc tattggatgg aacacagggc 166380 ctccaatgga ggagctagag aaagtaccca aggagctgaa gggatctgca accctataga 166440 tggaacaaga atatgaacta accagtaccc ccagaggtca tatctctagc tgcatatgta 166500 gcagaagatg gcctagtcgg ccatcagtgg gaagagaggc ccctaggtct tgcaaacttt 166560 atatgcccca gtacagggga acgccagagt caagaagtgg gagtgggtgg gtaggggatc 166620 agggtcggga gagggtatag ggaactttcg ggatagcatt tgaaatgtaa ataaagaaaa 166680 catctaataa aatagcaata aagaataacc actacaaaga gagaaaagga caacaaaagc 166740 ctgcagtaag tttgctagct gaacagagca gatgaataac cggcagcaga ctttgcacca 166800 catcatttcc tttggccatc cgcacagatt gccggaacag gcaaaccaga tctccatgca 166860 ttctgttgtc cccaccctct gggccatagg acttggacaa aggtaatgtc ctggatctga 166920 aagaagttaa gacactggga tggtatttaa ttaaataata acagaggctc aactcggtga 166980 ttggcctggc tggaccagcc tgatgtgttg ataattgtct aatcctctag tggatatcaa 167040 aaaaaatcat tgctttccca agggaagggg acagccagga gaaggagagg tatacatgga 167100 tgataaacgt tccagatact ggctaagtgc ttaccagtaa attgaagtcc acatttcaca 167160 gcatactcca ggaagtcatt tctccccagc cagactcaac agtctttgaa ggcttctaaa 167220 gtccagctgg gaggtagtgt tgagcctcta gcatgtctat tcatagacta ctagatccca 167280 tgagagctgc cattacaatt gctggagtac attttttcac ttcctatcac ctaaataaac 167340 ttcacccact gtgtgggttt gctaatccct tcctcatcct tgctgtatcc accaccctgt 167400 cttgtttcct tagcaactag acactgctga gggcagggca gaaggctaaa agggagcacc 167460 agtaagtgga atgagtgagt ggtggcttgg tgggctgcag aggggttagt tctcacgttg 167520 cctttttgag tattccccat cattttctct tctagtgcgc aagtgcaggg tcctcacatc 167580 tatccacact gtccacacag agagaacaca tttttaaaag ctagcccaaa gagagaacaa 167640 agagctgagg ataactattc ctaacccagg atgtctgcct tacattactt tttaggtact 167700 ttgcatttgt ttgctgctac tatcattgtt tgtttactat attttaggag ttcgaacaat 167760 cccaggcacc attcagacag aaagtggcaa atgtcatcta aaagggcagg gcttaacatt 167820 cccgtggcag tgtgtgcttc cccagtcaga gagagcactt tcttgctttt actgttttct 167880 aactcgagat ttcccccgct tttctggagc tttttttttt ttgttgttta gcttctcatc 167940 aagaaagtat aagttgtagg ctgcgggagt gtctgtgggg atgcgtttgt tttgtttttg 168000 agacagagtt tggctattta gagaaggcta gctttgaact cccagagacc cacctgcttg 168060 tctcaagtgc tggcactgaa agtatgtgtc cacatacctg gcctcgtgta ctaatttttt 168120 gataattaca aaaaagggca agaaaaaaag aaagaaagaa aatttctttc ttcagccatt 168180 cattctgtag cctgttgtgt ccaacactga tctaggcatt cttttggggt acttgagcag 168240 aaagactaga ctgtgtactt tctagctggg ctcttcacca gcaggtgcac atttcagtat 168300 ttatttggcc acttgaaaat ctcactgttg attttgtgat aaaaaaaaaa aacaaaaccc 168360 aggaacttat ttgtaaaacg attattcctg gtgtttatct gactcacctt atcatctgtg 168420 tttaaagatt ggtggggtac actctgagct atttgtactt ggcaagatcc ccaactctga 168480 gagcaaggtt ttagctggct agttttcctc cttcagcctc tgccaagtgc ttggtcctct 168540 agagaataat atgtaccttt tgtccccaca tattaaatac aaataaagag aagaacatgt 168600 catcttgagg cattggaagc tattaaggct atctcaggca atctggacaa ctctacgcct 168660 tggtcacata gtggcttctt aatatgtacc atggctcact caagaaaaat ggagtggtga 168720 ccaacttcag cctctgagtt ttgagatgga gactctgaga tagctgtcaa tattttgtaa 168780 aactgcttcc taacatcccg tacccctttc tgtatgtata tctgcatgca catgcacaca 168840 tatgggtata gagcccgcac tggccaaaag atgagagcag atctccctgg atccagaatt 168900 atatatggtt gtgagcttcc ctatattgct gctgagagcc aatccagttc tctggaagag 168960 caatactcac tctcaactgc tcagtcgcct ctacaaacca actgtttttt gtttgtttgt 169020 ttgtttgttt gtttgtctgt ttggtttggt ttggtttctc ttagacaggg tctctgactg 169080 ggaccgaggc ttgctgagta gggtaggcta tttgaccagc agccctcagg aatgcaagta 169140 ttttcctcaa catctgattt cctctttcct ttctccctcc ctccctccct ccctccctcc 169200 ctccctccct ccctcccttc cttaatttct tcctttcttc tttctttctt cctccttccc 169260 cctctcttcc cctctctctc tttctttttt tccattgccg acatgaagaa gtagagatga 169320 gcatgaccaa ggtcagtttc tgcttttcct gtgacaaggg ttctccccag tcaaccctgg 169380 tctgcccaca gaattcttat cccactgaaa ggaatagaca tttccctaca tgaagagttg 169440 taaagtggtg gttggtccca ttgcatgcac agagagtgag agacgggtaa cctgtgagac 169500 acctcctaca taaaaagagt tccagtgatc ttcaaagtgt ttgtcactca cccagaaaaa 169560 ctccagctcc tcactaccaa gcctaggaaa ggaagacaga aatacctaga gaggaattcc 169620 aatcacctca agaaaaatgg agtagtgacc agcttcagcc tctgagtttt gagatggaga 169680 ctctgagaga gctgtcaata ttttgtaaaa ccgtttccca ctcgatagca ttcccagagt 169740 gaaggttttt ctctcctcag ccatgagcta ggcttctgga tttctacatc ctgtttcatt 169800 tgtgttcttc ctcctatatt taagtgtaag cttgaatgag cgtctcagtt cacttcccac 169860 aatacgagaa tttcccaaac aaggagggca caccaaactc atgttagcag tgcctcttca 169920 ggtgttgccc atttttctcc ttcaaaagtc cccaagcctc ccaggtacta ctttgccagt 169980 tcaggttgtt caggggaggt acaccaagtg ctgattccca ctgagctcct actgacccta 170040 ttcagtattg tgccacagtt tacccttctc ttctcttctc ttctcttctc ttctcttctc 170100 ttctcttctc ttctcttctc ttctcttctc ttctcttctc ttctcttctc tcctctcctc 170160 tcctctcctc tcctctcctc tcctctcctc tcctctcctc tcctctcctc tcctctcctc 170220 tcctctcctc tcctttcccc tcccctcccc tccccctccc ctcccctccc ctccccctcc 170280 cctcccctcc cctcccctcc cctcccctcc cctcccctcc cctcccctcc cctccttttc 170340 ttttcttttc ttttcttttc ttttcttttc ttttcttttc ttttcttttc ttttcttttc 170400 ttttcttttc tttgtgatag gcttgggaag cttcccctgc aggactgtgg agctcttgtg 170460 ttcacatttg tgccttttgg aaagatgctt cagcggctgg acccgaggca agagtttgtc 170520 ttcaaaatgc tgccattgcc tctgtcagtt ttcatctgcc tgtaaacttt ttttttttct 170580 ttttgccaaa tctcctgaag caacttgacc tcaaggtttt ttttttttaa ttccctcaac 170640 tctttcttta ttctttgttc cattttcact atcttttttt tcttattagt taagagaaac 170700 caaagaattg attcacctgc tcattagttt tctcgaatat tcccctgtga ctaaagtaaa 170760 actaggctac acccctaaga gaaagatgcc tcttagggct cctgtcatct tcctattgtc 170820 ttgactggtg tgcatacaaa gtttgaacta tgaaactagg gcatggctga ttttggctcc 170880 agtgtttact tgtgaaccag tctattcact attgacctag ctttcagatt ccacagccct 170940 tctagcttcc ctaacataaa aacataagca ttatatgtat ttaggaagta tcatgttgtg 171000 ttccaataat atttatgcag tttgacctca gttgtgccct taatccttgt agtacctgtt 171060 ttagttaggg ttttactgct gtgaacaggc accatgacca agtcaactca taaaaacaac 171120 atttagtttg agctggctta taaattcaga ggttcccacc aatgtcatca aggtgggaac 171180 acgatagtgt tcaggccgat atggtgcaag aggagctgag agttgtacat tatcatagga 171240 aggctgctag cagaatagtg acttccgggc agctaggatg aagatcttaa agcccacacc 171300 cacaagagca cacctactcc aacaggacca caccttctaa tagtgccact ccctgggcca 171360 agcatattca aagcatcaca gtatcttaat tagaaaagtg ggagctatcc cttgttcatc 171420 tgtcttagtt gtcttgactg ctataacaaa acacctcaaa ctcattcatt cacaaag 171477 <210> 6 <211> 123 <212> DNA <213> Mus musculus <400> 6 atggggaccc gggcaagcag catcaccgcc ctcgcctctt gcagccgcac cgccggccaa 60 gtcggagcca cgatggtcgc cggctctctt ctcctgcttg gattcctcag caccatcaca 120 gct 123 <210> 7 <211> 96 <212> DNA <213> Mus musculus <400> 7 tggtacattc tggtgccaag tgtggccatt cccctggcta tcgccttcct cttcttcttc 60 atctgtgtgt gccgcaataa ccagaagtct tcatca 96 <210> 8 <211> 3075 <212> DNA <213> Escherichia coli <400> 8 ggtaccgatt taaatgatcc agtggtcctg cagaggagag attgggagaa tcccggtgtg 60 acacagctga acagactagc cgcccaccct ccctttgctt cttggagaaa cagtgaggaa 120 gctaggacag acagaccaag ccagcaactc agatctttga acggggagtg gagatttgcc 180 tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct tcctgaggcc 240 gatactgtcg tcgtcccctc aaactggcag atgcacggtt acgatgcgcc catctacacc 300 aacgtgacct atcccattac ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt 360 tgttactcgc tcacatttaa tgttgatgaa agctggctac aggaaggcca gacgcgaatt 420 atttttgatg gcgttaactc ggcgtttcat ctgtggtgca acgggcgctg ggtcggttac 480 ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg catttttacg cgccggagaa 540 aaccgcctcg cggtgatggt gctgcgctgg agtgacggca gttatctgga agatcaggat 600 atgtggcgga tgagcggcat tttccgtgac gtctcgttgc tgcataaacc gactacacaa 660 atcagcgatt tccatgttgc cactcgcttt aatgatgatt tcagccgcgc tgtactggag 720 gctgaagttc agatgtgcgg cgagttgcgt gactacctac gggtaacagt ttctttatgg 780 cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag 840 cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg 900 agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg 960 ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg tgcggattga aaatggtctg 1020 ctgctgctga acggcaagcc gttgctgatt cgaggcgtta accgtcacga gcatcatcct 1080 ctgcatggtc aggtcatgga tgagcagacg atggtgcagg atatcctgct gatgaagcag 1140 aacaacttta acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg gtacacgctg 1200 tgcgaccgct acggcctgta tgtggtggat gaagccaata ttgaaaccca cggcatggtg 1260 ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg 1320 cgaatggtgc agcgcgatcg taatcacccg agtgtgatca tctggtcgct ggggaatgaa 1380 tcaggccacg gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc 1440 cgcccggtgc agtatgaagg cggcggagcc gacaccacgg ccaccgatat tatttgcccg 1500 atgtacgcgc gcgtggatga agaccagccc ttcccggctg tgccgaaatg gtccatcaaa 1560 aaatggcttt cgctacctgg agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg 1620 atgggtaaca gtcttggcgg tttcgctaaa tactggcagg cgtttcgtca gtatccccgt 1680 ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc tgattaaata tgatgaaaac 1740 ggcaacccgt ggtcggctta cggcggtgat tttggcgata cgccgaacga tcgccagttc 1800 tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa 1860 caccagcagc agtttttcca gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa 1920 tacctgttcc gtcatagcga taacgagctc ctgcactgga tggtggcgct ggatggtaag 1980 ccgctggcaa gcggtgaagt gcctctggat gtcgctccac aaggtaaaca gttgattgaa 2040 ctgcctgaac taccgcagcc ggagagcgcc gggcaactct ggctcacagt acgcgtagtg 2100 caaccgaacg cgaccgcatg gtcagaagcc gggcacatca gcgcctggca gcagtggcgt 2160 ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc acgccatccc gcatctgacc 2220 accagcgaaa tggatttttg catcgagctg ggtaataagc gttggcaatt taaccgccag 2280 tcaggctttc tttcacagat gtggattggc gataaaaaac aactgctgac gccgctgcgc 2340 gatcagttca cccgtgcacc gctggataac gacattggcg taagtgaagc gacccgcatt 2400 gaccctaacg cctgggtcga acgctggaag gcggcgggcc attaccaggc cgaagcagcg 2460 ttgttgcagt gcacggcaga tacacttgct gatgcggtgc tgattacgac cgctcacgcg 2520 tggcagcatc aggggaaaac cttatttatc agccggaaaa cctaccggat tgatggtagt 2580 ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg 2640 attggcctga actgccagct ggcgcaggta gcagagcggg taaactggct cggattaggg 2700 ccgcaagaaa actatcccga ccgccttact gccgcctgtt ttgaccgctg ggatctgcca 2760 ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg 2820 cgcgaattga attatggccc acaccagtgg cgcggcgact tccagttcaa catcagccgc 2880 tacagtcaac agcaactgat ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc 2940 acatggctga atatcgacgg tttccatatg gggattggtg gcgacgactc ctggagcccg 3000 tcagtatcgg cggaattcca gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt 3060 caaaaataat aataa 3075 <210> 9 <211> 249 <212> DNA <213> Artificial <220> <223> Synthetic <400> 9 gtaatgatca taatcagcca tatcacatct gtagaggttt tacttgcttt aaaaaacctc 60 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 120 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 180 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 240 tggatcccc 249 <210> 10 <211> 34 <212> DNA <213> Bacteriophage P1 <400> 10 ataacttcgt ataatgtatg ctatacgaag ttat 34 <210> 11 <211> 1213 <212> DNA <213> Homo sapiens <400> 11 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg 60 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag 120 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag 180 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg 240 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat 300 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt 360 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct 420 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc 480 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg 540 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga 600 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt 660 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct 720 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt 780 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc 840 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg 900 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat 960 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg 1020 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg 1080 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca 1140 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct 1200 tttttgttag acg 1213 <210> 12 <211> 804 <212> DNA <213> Escherichia coli <400> 12 atgggatcgg ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag 60 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc 120 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg 180 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc 240 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg 300 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct 360 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg 420 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat 480 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc 540 atgcccgacg gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg 600 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc 660 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct 720 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat 780 cgccttcttg acgagttctt ctga 804 <210> 13 <211> 477 <212> DNA <213> Artificial <220> <223> Synthetic <400> 13 gctgtaagtc tgcagaaatt gatgatctat taaacaataa agatgtccac taaaatggaa 60 gtttttcctg tcatactttg ttaagaaggg tgagaacaga gtacctacat tttgaatgga 120 aggattggag ctacgggggt gggggtgggg tgggattaga taaatgcctg ctctttactg 180 aaggctcttt actattgctt tatgataatg tttcatagtt ggatatcata atttaaacaa 240 gcaaaaccaa attaagggcc agctcattcc tcccactcat gatctataga tctatagatc 300 tctcgtggga tcattgtttt tctcttgatt cccactttgt ggttctaagt actgtggttt 360 ccaaatgtgt cagtttcata gcctgaagaa cgagatcagc agcctctgtt ccacatacac 420 ttcattctca gtattgtttt gccaagttct aattccatca gacctcgacc tgcagcc 477 <210> 14 <211> 3626 <212> DNA <213> Mus musculus <220> <221> CDS <222> (443)..(2407) <220> <221> sig_peptide <222> (443)..(565) <220> <221> mat_peptide <222> (566)..(2407) <400> 14 gtgtccccga gctgagtggc catccgactc agtccctcgc cggccggtct aggcagcgga 60 ggaggcgagt gcttgatagt ggctggaagc ttcgctatgg gaagtcgctc tttagcactg 120 tcgcggctag ccctgctctc tggttctccg cagccgctgt cgttggagag caccgggagg 180 cgcgggttgc gagcgcgcct gcttctcacc gcccgggcgc cagcgcccct gggcaggtgc 240 tgagcgcctt tcggagcctc ccctgctgcc tccctcttcc gcctgggtgc ctggctgctg 300 cagtgcacat gggctgctgg aggtagatgg gctcaccgcc cgtgaggcgg cggtggatgc 360 ggcgctgggc agaaacagcc accaattcca gctgccgtgg ggccgagcgc cccgggcgcc 420 gctgcgagcc ccgagctcgg cc atg ggg acc cgg gca agc agc atc acc gcc 472 Met Gly Thr Arg Ala Ser Ser Ile Thr Ala -40 -35 ctc gcc tct tgc agc cgc acc gcc ggc caa gtc gga gcc acg atg gtc 520 Leu Ala Ser Cys Ser Arg Thr Ala Gly Gln Val Gly Ala Thr Met Val -30 -25 -20 gcc ggc tct ctt ctc ctg ctt gga ttc ctc agc acc atc aca gct caa 568 Ala Gly Ser Leu Leu Leu Leu Gly Phe Leu Ser Thr Ile Thr Ala Gln -15 -10 -5 -1 1 cca gaa caa aag act ctg agt ctc cct ggc acc tac cgc cat gtt gac 616 Pro Glu Gln Lys Thr Leu Ser Leu Pro Gly Thr Tyr Arg His Val Asp 5 10 15 cgt acc act ggc cag gtg cta acc tgc gac aag tgc cca gca gga acg 664 Arg Thr Thr Gly Gln Val Leu Thr Cys Asp Lys Cys Pro Ala Gly Thr 20 25 30 tac gtc tcc gag cac tgt acc aac atg agc ctg cga gtc tgc agc agc 712 Tyr Val Ser Glu His Cys Thr Asn Met Ser Leu Arg Val Cys Ser Ser 35 40 45 tgc ccc gcg ggg acc ttt acc agg cac gag aac ggc ata gag aga tgc 760 Cys Pro Ala Gly Thr Phe Thr Arg His Glu Asn Gly Ile Glu Arg Cys 50 55 60 65 cat gac tgt agt cag cca tgt cca tgg ccg atg att gag aga tta cct 808 His Asp Cys Ser Gln Pro Cys Pro Trp Pro Met Ile Glu Arg Leu Pro 70 75 80 tgt gct gcc ttg act gac cga gag tgc atc tgc cca cct gga atg tat 856 Cys Ala Ala Leu Thr Asp Arg Glu Cys Ile Cys Pro Pro Gly Met Tyr 85 90 95 cag tct aat ggt acc tgc gct ccc cat aca gtg tgc ccc gtg ggc tgg 904 Gln Ser Asn Gly Thr Cys Ala Pro His Thr Val Cys Pro Val Gly Trp 100 105 110 ggt gtg cgg aag aaa ggg aca gag aat gaa gat gtg cgc tgt aag cag 952 Gly Val Arg Lys Lys Gly Thr Glu Asn Glu Asp Val Arg Cys Lys Gln 115 120 125 tgc gct cgg ggt acc ttc tct gac gtg cct tcc agt gtg atg aag tgt 1000 Cys Ala Arg Gly Thr Phe Ser Asp Val Pro Ser Ser Val Met Lys Cys 130 135 140 145 aaa gct cac acg gac tgt ctg ggt cag aac ctg gag gtg gtc aag cca 1048 Lys Ala His Thr Asp Cys Leu Gly Gln Asn Leu Glu Val Val Lys Pro 150 155 160 ggg acc aag gag aca gac aac gtc tgt ggc atg cgc ctg ttc ttc tcc 1096 Gly Thr Lys Glu Thr Asp Asn Val Cys Gly Met Arg Leu Phe Phe Ser 165 170 175 agc aca aac cca cct tcc tct ggc aca gtt acc ttt tct cac cct gag 1144 Ser Thr Asn Pro Pro Ser Ser Gly Thr Val Thr Phe Ser His Pro Glu 180 185 190 cat atg gaa tcc cac gat gtc cct tcc tcc acc tat gag ccc caa ggc 1192 His Met Glu Ser His Asp Val Pro Ser Ser Thr Tyr Glu Pro Gln Gly 195 200 205 atg aac tca aca gat tcc aac tct act gcc tct gtt aga act aag gta 1240 Met Asn Ser Thr Asp Ser Asn Ser Thr Ala Ser Val Arg Thr Lys Val 210 215 220 225 cca agt ggc atc gag gaa ggg aca gtg cct gac aat acg agc tca acc 1288 Pro Ser Gly Ile Glu Glu Gly Thr Val Pro Asp Asn Thr Ser Ser Thr 230 235 240 agt ggg aag gaa ggc act aat agg acc ctg cca aac cca cca caa gtt 1336 Ser Gly Lys Glu Gly Thr Asn Arg Thr Leu Pro Asn Pro Pro Gln Val 245 250 255 acc cac cag caa gcc ccc cac cac aga cac att ctg aag ctg ctg cca 1384 Thr His Gln Gln Ala Pro His His Arg His Ile Leu Lys Leu Leu Pro 260 265 270 tcg tcc atg gag gcc acg ggt gag aag tcc agc aca gcc atc aag gcc 1432 Ser Ser Met Glu Ala Thr Gly Glu Lys Ser Ser Thr Ala Ile Lys Ala 275 280 285 ccc aag agg ggt cac ccc aga cag aac gct cac aag cat ttc gac atc 1480 Pro Lys Arg Gly His Pro Arg Gln Asn Ala His Lys His Phe Asp Ile 290 295 300 305 aac gag cac ttg cct tgg atg atc gtc ctc ttc ctt ctg ctg gtc ctg 1528 Asn Glu His Leu Pro Trp Met Ile Val Leu Phe Leu Leu Leu Val Leu 310 315 320 gtg ctg ata gtg gtg tgc agt atc cga aag agc tcc agg act ctc aaa 1576 Val Leu Ile Val Val Cys Ser Ile Arg Lys Ser Ser Arg Thr Leu Lys 325 330 335 aag ggg ccc cgg cag gat ccc agc gcc ata gtg gaa aag gcg ggg ctg 1624 Lys Gly Pro Arg Gln Asp Pro Ser Ala Ile Val Glu Lys Ala Gly Leu 340 345 350 aag aag tcc ctg act ccc acc cag aac cgg gag aaa tgg atc tac tac 1672 Lys Lys Ser Leu Thr Pro Thr Gln Asn Arg Glu Lys Trp Ile Tyr Tyr 355 360 365 cgc aac ggc cat ggt att gac atc ttg aag ctt gta gca gcc cag gtg 1720 Arg Asn Gly His Gly Ile Asp Ile Leu Lys Leu Val Ala Ala Gln Val 370 375 380 385 gga agc cag tgg aag gac atc tat cag ttt ctt tgc aac gcc agc gag 1768 Gly Ser Gln Trp Lys Asp Ile Tyr Gln Phe Leu Cys Asn Ala Ser Glu 390 395 400 agg gag gtg gcg gcc ttc tcc aat gga tac act gca gat cat gaa cgg 1816 Arg Glu Val Ala Ala Phe Ser Asn Gly Tyr Thr Ala Asp His Glu Arg 405 410 415 gcc tac gcg gct ctg cag cac tgg acc atc cgt ggc cct gag gcc agc 1864 Ala Tyr Ala Ala Leu Gln His Trp Thr Ile Arg Gly Pro Glu Ala Ser 420 425 430 ctt gcc cag ctc att agc gcc ttg cgc cag cac cga cgc aat gat gtt 1912 Leu Ala Gln Leu Ile Ser Ala Leu Arg Gln His Arg Arg Asn Asp Val 435 440 445 gtg gag aag att cgt ggg ctg atg gaa gac acc acg cag ttg gaa aca 1960 Val Glu Lys Ile Arg Gly Leu Met Glu Asp Thr Thr Gln Leu Glu Thr 450 455 460 465 gac aaa ctg gct ctc ccc atg agc ccc agt ccg ctt agc ccg agc ccc 2008 Asp Lys Leu Ala Leu Pro Met Ser Pro Ser Pro Leu Ser Pro Ser Pro 470 475 480 atg ccc agt cct aac gtg aaa ctt gag aat tcc act ctc ctg aca gtg 2056 Met Pro Ser Pro Asn Val Lys Leu Glu Asn Ser Thr Leu Leu Thr Val 485 490 495 gag ccc tca ccg ctg gac aag aac aag tgc ttc ttc gtg gac gag tca 2104 Glu Pro Ser Pro Leu Asp Lys Asn Lys Cys Phe Phe Val Asp Glu Ser 500 505 510 gag ccc ctt ctg cga tgc gac tcc aca tcc agt ggc tct tca gca ctg 2152 Glu Pro Leu Leu Arg Cys Asp Ser Thr Ser Ser Gly Ser Ser Ala Leu 515 520 525 agc aga aac ggc tcc ttt att acc aaa gaa aag aag gac aca gtg ttg 2200 Ser Arg Asn Gly Ser Phe Ile Thr Lys Glu Lys Lys Asp Thr Val Leu 530 535 540 545 cgg cag gtc cgc ctg gac ccc tgt gac ttg cag ccc atc ttt gat gac 2248 Arg Gln Val Arg Leu Asp Pro Cys Asp Leu Gln Pro Ile Phe Asp Asp 550 555 560 atg ctg cat atc ctg aac ccc gag gag ctg cgg gtg att gaa gag att 2296 Met Leu His Ile Leu Asn Pro Glu Glu Leu Arg Val Ile Glu Glu Ile 565 570 575 ccc cag gct gag gac aaa ctg gac cgc ctc ttc gag atc att ggg gtc 2344 Pro Gln Ala Glu Asp Lys Leu Asp Arg Leu Phe Glu Ile Ile Gly Val 580 585 590 aag agc caa gaa gcc agc cag acc ctc ttg gac tct gtg tac agt cat 2392 Lys Ser Gln Glu Ala Ser Gln Thr Leu Leu Asp Ser Val Tyr Ser His 595 600 605 ctt cct gac cta ttg tagaacacag gggcactgca ttctgggaat caacctactg 2447 Leu Pro Asp Leu Leu 610 gcggggtgat ttcatttcgt ttctgacttt tgtgttttgg tgtgtatgta tgtgtttaac 2507 agagtgtatg gccggtgagt ttgggttctt tctttctttc tttctttctt tctttctttc 2567 tttctttctt tctttctttc tttctttctt tcttccttcc tgaaagtgaa tgtataaagc 2627 ctttacaatg tataactgtt ggaaaatgcc caccactaaa ttttttttta agttccacat 2687 attctccatt tttgccttct tatatatatc ttcaacacta ttctgtgcac tttaaaaact 2747 taacataaac gcagtgtgac ttctcccata tgctgggtcc cgagactctc aacttcttaa 2807 aaacctaatg gcatcttgtg actcctagaa gtagacataa gtctttcaac cttcacacct 2867 actctttctg ttttaattat tattgctatt tgtcttattg tttgtgcttt acaagcgttc 2927 ttgaggacgg agggaatcta cgaccctgtt gatgactgta actctattcg actttgagtt 2987 gtcttcttca tgtcttgtta tatagttcat attcatggct gaaacttgac catactccct 3047 agcgctgatt gtatggtttt cgtctggaca ccgtacactg cctgataact tgtgcacctc 3107 ttaacgctac tatgctctgg gctggagaat gaaatcttta agtcaccagg acttgctgtt 3167 tcagtggctt gacacctggg ccaccaaaga actcgatctt catcttttag ggacacctct 3227 gctgcacctt ggaaagccaa ccttaagtgc cagtggcact ttatgcccag ctttgctttg 3287 aaagatatct ttcttgtttt ttttatcctt ctctttctct ctttttttta aaaatacaca 3347 tagtcaatag gtccagtctg ccctcaaggc cttgctgggt tttcttcatc atccaatcac 3407 tttcattaaa aatggctgca gctgtaagaa ctcttgtctg ataaattttc aactatgctc 3467 tcatttatct acctgccctc tgatgctcag tcgtcagact ctaatgcaaa ggtggacgtc 3527 ggctgccttt gcgtgggcgg gcttagtggt gaggaactga tatcagaaaa aaaatgcctt 3587 caagtatact aatttattaa taaatattag gtgtttgtt 3626 <210> 15 <211> 655 <212> PRT <213> Mus musculus <400> 15 Met Gly Thr Arg Ala Ser Ser Ile Thr Ala Leu Ala Ser Cys Ser Arg -40 -35 -30 Thr Ala Gly Gln Val Gly Ala Thr Met Val Ala Gly Ser Leu Leu Leu -25 -20 -15 -10 Leu Gly Phe Leu Ser Thr Ile Thr Ala Gln Pro Glu Gln Lys Thr Leu -5 -1 1 5 Ser Leu Pro Gly Thr Tyr Arg His Val Asp Arg Thr Thr Gly Gln Val 10 15 20 Leu Thr Cys Asp Lys Cys Pro Ala Gly Thr Tyr Val Ser Glu His Cys 25 30 35 Thr Asn Met Ser Leu Arg Val Cys Ser Ser Cys Pro Ala Gly Thr Phe 40 45 50 55 Thr Arg His Glu Asn Gly Ile Glu Arg Cys His Asp Cys Ser Gln Pro 60 65 70 Cys Pro Trp Pro Met Ile Glu Arg Leu Pro Cys Ala Ala Leu Thr Asp 75 80 85 Arg Glu Cys Ile Cys Pro Pro Gly Met Tyr Gln Ser Asn Gly Thr Cys 90 95 100 Ala Pro His Thr Val Cys Pro Val Gly Trp Gly Val Arg Lys Lys Gly 105 110 115 Thr Glu Asn Glu Asp Val Arg Cys Lys Gln Cys Ala Arg Gly Thr Phe 120 125 130 135 Ser Asp Val Pro Ser Ser Val Met Lys Cys Lys Ala His Thr Asp Cys 140 145 150 Leu Gly Gln Asn Leu Glu Val Val Lys Pro Gly Thr Lys Glu Thr Asp 155 160 165 Asn Val Cys Gly Met Arg Leu Phe Phe Ser Ser Thr Asn Pro Pro Ser 170 175 180 Ser Gly Thr Val Thr Phe Ser His Pro Glu His Met Glu Ser His Asp 185 190 195 Val Pro Ser Ser Thr Tyr Glu Pro Gln Gly Met Asn Ser Thr Asp Ser 200 205 210 215 Asn Ser Thr Ala Ser Val Arg Thr Lys Val Pro Ser Gly Ile Glu Glu 220 225 230 Gly Thr Val Pro Asp Asn Thr Ser Ser Thr Ser Gly Lys Glu Gly Thr 235 240 245 Asn Arg Thr Leu Pro Asn Pro Pro Gln Val Thr His Gln Gln Ala Pro 250 255 260 His His Arg His Ile Leu Lys Leu Leu Pro Ser Ser Met Glu Ala Thr 265 270 275 Gly Glu Lys Ser Ser Thr Ala Ile Lys Ala Pro Lys Arg Gly His Pro 280 285 290 295 Arg Gln Asn Ala His Lys His Phe Asp Ile Asn Glu His Leu Pro Trp 300 305 310 Met Ile Val Leu Phe Leu Leu Leu Val Leu Val Leu Ile Val Val Cys 315 320 325 Ser Ile Arg Lys Ser Ser Arg Thr Leu Lys Lys Gly Pro Arg Gln Asp 330 335 340 Pro Ser Ala Ile Val Glu Lys Ala Gly Leu Lys Lys Ser Leu Thr Pro 345 350 355 Thr Gln Asn Arg Glu Lys Trp Ile Tyr Tyr Arg Asn Gly His Gly Ile 360 365 370 375 Asp Ile Leu Lys Leu Val Ala Ala Gln Val Gly Ser Gln Trp Lys Asp 380 385 390 Ile Tyr Gln Phe Leu Cys Asn Ala Ser Glu Arg Glu Val Ala Ala Phe 395 400 405 Ser Asn Gly Tyr Thr Ala Asp His Glu Arg Ala Tyr Ala Ala Leu Gln 410 415 420 His Trp Thr Ile Arg Gly Pro Glu Ala Ser Leu Ala Gln Leu Ile Ser 425 430 435 Ala Leu Arg Gln His Arg Arg Asn Asp Val Val Glu Lys Ile Arg Gly 440 445 450 455 Leu Met Glu Asp Thr Thr Gln Leu Glu Thr Asp Lys Leu Ala Leu Pro 460 465 470 Met Ser Pro Ser Pro Leu Ser Pro Ser Pro Met Pro Ser Pro Asn Val 475 480 485 Lys Leu Glu Asn Ser Thr Leu Leu Thr Val Glu Pro Ser Pro Leu Asp 490 495 500 Lys Asn Lys Cys Phe Phe Val Asp Glu Ser Glu Pro Leu Leu Arg Cys 505 510 515 Asp Ser Thr Ser Ser Gly Ser Ser Ala Leu Ser Arg Asn Gly Ser Phe 520 525 530 535 Ile Thr Lys Glu Lys Lys Asp Thr Val Leu Arg Gln Val Arg Leu Asp 540 545 550 Pro Cys Asp Leu Gln Pro Ile Phe Asp Asp Met Leu His Ile Leu Asn 555 560 565 Pro Glu Glu Leu Arg Val Ile Glu Glu Ile Pro Gln Ala Glu Asp Lys 570 575 580 Leu Asp Arg Leu Phe Glu Ile Ile Gly Val Lys Ser Gln Glu Ala Ser 585 590 595 Gln Thr Leu Leu Asp Ser Val Tyr Ser His Leu Pro Asp Leu Leu 600 605 610 <210> 16 <211> 168823 <212> DNA <213> Artificial <220> <223> Synthetic <400> 16 ggtgttcctt ggcaactcac tttttctttt taaaaattgt gtgtgtgtgc tgtgtgtgta 60 tgtgtgagtg ggtaagtagg tgtacagcag ccaggggagg atctcagatg tcttccccta 120 tcattctgcc ttgtgccttg tgcctttgag gcagaacatc ttggtgaacc cagagcttat 180 atatgtagac acacacacag atatataaac acattatatg tatataaata tatataatgt 240 gataccataa tatagatata tacacaagac acacacacat atatatgtaa tataatatat 300 gtaatttata tacacatatg tatatgaata tatatatata atatgctacc ataatataga 360 catatactag acacacatat atgtaatata tacaaatatg tatataaata tataatttat 420 gctatcataa tatatatacc ctagacatat atgtataata taacacatat atgtgatata 480 tgtgtgtgtg tgtatataca tatatatata tatatcacgc acacacacat acatacgtct 540 agggtagcaa ccaatcctag taatcctcct gttttcattc ccctcaatgg tctacaggca 600 tgtatgagac cacatatggc ttttatgggt gtatgtgtga tctaaactca gaccttcacg 660 tttgcataat aatcactgtt aactgctgag ccatctctcc ttctctcact ttcctttctt 720 tttgaaaaaa aattttttat acattatgtt tttattttat tttacttatt tttatttttt 780 taatattaga tttttttctt tatttacatt tcaaatgtta tcccctttcc tggtttcccc 840 tccaaaaacc cctatcccat ccccactccc cctgttcacc aacccaccca ctcgcttccc 900 tgtcctggca tgcccctaca ctggggcata tacattaggt tttgagcgaa ttccaggata 960 gccagggcta cacagagaaa ccttgtcttg aattaaaaaa aatttttttt ttaaattaga 1020 gttgctgcca tgagtatgag caacttaaga aaattatctc tcgccgggca gtggtggcac 1080 acgcctttaa tcccaacact tgggaggcag aggcaggtgg atttctgagt tcaaggtcag 1140 cctggtctac agagtgagtt ccaggacagc cagagctaca cagagaaacc ctataaaaaa 1200 ataacacaca cacaaaaaaa atcaaaaaca aaaacaaaca agagagagag agagagagag 1260 agagattctt tccctccaac ttctcccaga ccttccctac ctccattccc accaacttca 1320 tgtttttttt tctctctctc tctttctcaa aaccaaaaaa tgccaggaaa gcaacaaaaa 1380 ataagtagca agaaagtcag aacaaagaaa ttaaatacgt aagtaagtaa ataaaaatag 1440 aaaatcagca atattaaaaa aaaatcaagg actggagaga tggctcagca gttaagagca 1500 ctgattgctc ttccagaggt cctgagttca aatctcagca accacatagt ggctcacaaa 1560 catctgtaat gggatccgat gccctcttct ggtgtgtctg aagagagcta cagtgtactt 1620 acgtttaata aataaatctt taaaacaaga caacacaaaa atccaaaata aaatttcaaa 1680 gtgtaccaac acaaaacatg tagtctgttt tgtgttggtc aactactcct gggtatttag 1740 gcttccttgg agtgtggttg ctatacccag tgacactcag ttaagaaaag tgaatttccc 1800 tttcctggca gggatcaatt gcaagtagct ttttggttag gggtagaata ttatattcca 1860 ccttcctgtg ctggttccac ctgtaaatgt ctgtgcagag ctgtttatgt ttacgtctca 1920 ggcagagctc tgaggaagat attagtaagc aagacattcc ttggagagtg cttgggggat 1980 ccagcaactg tgaaaggaaa aagaaatatt aatcaaaggg ataaattaac cttcaataca 2040 gtttcagcaa aggcctctca tggtgctgtc cccagttgag gcaaggcaac agaccttttt 2100 cctcaccaaa cactcactgg tgtaggggca tggtatgacc tgtcatcagg cagggaacat 2160 tcaccacaca gagtggagag gagctgtggc catctcttca atcagagcag ggtgttggat 2220 ggacgttgcc agcctgtacc acccaccatt cacatcagct gctctgtgca cacagcacac 2280 tccaacccac agagaaggtt tgtttctgtt tttatttttg ctttttatta ttatctcatg 2340 taaaattatt atttatttta tttttgcttt ttttattatt atctcatgta acccaggttg 2400 gctttaaatt tgttatgtag tcaaggagga ctttgaactt cttccctcac ctctagagtg 2460 ctggggtcac aggcacgtat cactattcct gttcacctgg tacttggaat caaacccagg 2520 cttcatgaat aatagaccta aactcttcca aaccaagaag aaccacatct cacgagattt 2580 gacatgctgt gggttttttt ttttcctctc ccccctctgt ctctctcctc ttctgtccct 2640 ctttctccct cttctttatt tctttcattt ttatttttac ttttgaaaag aagctggctt 2700 caaaattctc catatggctg aggacgactt cagcctttaa tttctttttt tgtttgtttg 2760 tttgtttttc taggcagggt ctcactacac agccctggct gtccttgaac tcactatgca 2820 gactaggctg gcctcgaacc tggagatctg cttgcctctg cctcctcagt gctgaaattg 2880 aagacttgca ccaccaaggt atttttagtt aatcgtttga aaaatttata catgtttaca 2940 atgtattttg atcaaatcca acagaaatct tcctctcact tcttctcagt ccccctacat 3000 ctttcctacc ataatttctc tctttctctt ctttttattt ttgaaataac ccaccaagtc 3060 cagatagtac tgtctacaag tgcataaatg tagagccatc ggctaaagca tggccaggct 3120 actagtaatc ccactgaaca ttacacacgt tctttggtca aattaagcaa gcttaatttt 3180 ctgttagaca gggatagctc cctaaatcag actttgataa tatagcatga cacttaagag 3240 aaagtggggc ttgtagagcc tccaaaacct gcaagcctgg gggtttttta aggcttggga 3300 gacttccaga ggaattttga ttgcatagga acttggctag cagaacatct tatcttatca 3360 gggtgcattc aaggtgtgag tcaggctcca gagggtgggg agcaagtcgg attccagaaa 3420 cacacagtga gtgtttggtg aggaaaaagc acagtcaacc tgaattttgc agcagacaga 3480 aatgaacttc tgaccttata acaaaatggc ttccgtcctt aagatggcgt caggctggtc 3540 catcagtaac tacacccctg aagaaaagtt atttcctctc cctaactgtc accaattgcc 3600 agcaacttct cagctaggaa tagagcctca tgatttcctc tcccactcat actgggtgtt 3660 gactggcttg agtgccatgc cacgtcctga agacagcatt tcacagcatt tgtccctatc 3720 ctctggctcc tacggttctt ttgttccctc tgctgagatg taggtacccc gagccttgta 3780 gtagaagtga caggcatgtc ccatttatgg atgactactc aatggtcaca tactctcaga 3840 actgtggcca gttatgagtc tgtgttgact gctgcctact gcaaaaggag tcttctctgg 3900 gcaaaacagt agtaatctac atgtataaaa cacaaatgct tacaaggtag tttgatgggg 3960 gggggggact gtcaaacaac ccttgcacag gggtcaccta ataccatctg cacaggggat 4020 atttgtgcct tccctcagcc ttgagtaggc taaacaaacc catgatagtt ttacaaccta 4080 gctggagtct tctgacctca ccacatttgc aatttcctat agagctttga ggagtagact 4140 gcccactgag cttgctttgc aacataatct ggctgaaacg aaggatgggg tttgtgggct 4200 ttctcagtgc tgaaaattaa ctttgagcct tgatcagaaa cgtttgtctt tgcttcatcc 4260 ttctcttgca cccagttttt ttctcagttc caggcccccc cccgccccgc ccctttcagg 4320 tatacccagt ttgtccatgg ccactggaca gctacagata tttacattac gattcacaac 4380 agtagcaaaa ttagttatgg agtagcattg aaatagtttt atggttgggc atcaccataa 4440 cacgaggaac tgtcttaaag ggtcacagct ttaggaaggc tgagaaccac tgtcctgcag 4500 cctagctctc atgggtcctt gataaggttt gtagcaccag acatgaactt tttcctgtgg 4560 agcagccctc aaagacagtc agaaagagca gttggttatc ccaacaacca acatgtcact 4620 attatatcaa gggggctttc ctatagcgtg aatggtcctc agagggtaag tccatctttt 4680 ctccccacgt cgcagggata gcatttcctg gcaccatgaa agcaggccag tagaaaggaa 4740 gtttctaagg accttgtact tctgatcctc ctcctcgcat gtgttgggat tacaggcctg 4800 caccaccaca tccagtttat taggggctag gcatgtgcac agtaagccaa cactcaataa 4860 gcagaacgac actcagacct cagccccttc atttagtgtt agtgtgttgg tggttgttta 4920 ttattattat tattattatt aattcaccac acagcaattt atgtcctaca tgtcacattc 4980 atttttaaat tccctttact ttttccccta ctttgtttta gaatatatgt atatgtgtat 5040 gtgtatacac gcacacacac acatacatat atatgtgtat acacacatac atatatgtgt 5100 attcatgcat atacatatgt gaatatacac acatacacac gtatatatga gtatacacac 5160 atatacatat ataatatgtg cgcacacata tattttatat attagcattc cagcttaggg 5220 gcttcagtgt agctcattgg cacagctctt gtctagcgtt ccagttttat ttctactttt 5280 ggcttttaag tctatttgtt ctattgattt aaagcttaca gtacctcggt gggggcacac 5340 acctttaatc ctagcactct ggaggcaaag gcaggtggat ctccgagttt gaggccatcc 5400 tggtctacag atcgagttcc aggataaacc gaaagtagag aaaccatgtc tcagaaacca 5460 aaagctaaaa caaacagaaa agattcacaa caaatatttt taaatcatta caatatatta 5520 caatatcatt aatattacac ccctttaagg gtagtataag aatctttatg ttatgtgtct 5580 ataaaatggg ttgaggagct ggtgagatgg ttcagtggat gaaggagctt gttagcaagc 5640 ctgccaactt gagttcaatc cctaggaccc acgtgataaa aggagggagt agattctttg 5700 aagttgtcct ctgacctcca catgtatact atgtcacatg tgttcggcat aaaataaaca 5760 aaaacataga agggagccta ggagacagtg ccgttggtac aaagcctgct gcaagagcat 5820 gaagccctga gtttgagccc tagcactcgg tcttatgcat acagcacatg tctgtaatct 5880 cagcactggg gagacaaaag agtgagtggc cctggagttt gctgggctgc cagtcaagcc 5940 aaatcgacta gctccagata gggagtgggg cagagaaaga ctgagcccat ctcaatggtg 6000 ggcaatgatt gataaagaca tccagtcttg ccttctggct tcccataagt gcatccctca 6060 gtgatatact gttacctgga agagtaagcc aaccaagccc tttcctccct aactgatttt 6120 ggttggaaag gaggccattt ccatgaagct aggaattgac cttggatgga agatgctggc 6180 ggaagtttct ggtttagtcc aggtggcccc cgaagtgtgg gctacaccat ttcccagaaa 6240 cctctagtag gcatgcactc cagctaccca tgctggacac tcgttctttg atctctctcg 6300 tttactttct tattttcctt atattatttg ctcgtttatc cccagacatt cctgggactg 6360 tcttttacac aaattcttca catcccatga tgtctttccg gctctccgtc ttggaagaaa 6420 acacccaagt gactgactgt gaccaaacgg ttacatgcaa atgtatgaaa tctggggacg 6480 aatgcagtaa taaaaggtga cttaaagggg ctggagtgat ggttcagtgg ataagagcac 6540 tgactgcact tccagaggtc ctgagttcaa ttcccagcaa ccacatggtt cacaaccatc 6600 tataatggga cccgatgccc tcttgtggtg tttctgagga tagcaacagt gtactcatat 6660 aaataaatca atcttaaaag aaaataaaaa acatttcatt aaactcgata ataggtataa 6720 atgcgaactt aatggatgtt ctcagggaag ttgactgtaa cttgtaattt gttcagtgaa 6780 tagcaagcat ataacaacca gacggctaaa actaccacac cgctaccaca catgtttctt 6840 tacgtatcac atctgcattt ccccttgcct caagcatagt acttggaact ttgttcctca 6900 tattttatga ctgccttcat ttttttagaa aagagttgtt actattatta tatgtaagta 6960 cactgtagct gtctttagac acaccagaag agggcatcat gtggttgctg ggatttgaac 7020 tcaggatctc cagaagagca gtcagtgctc ttaactgctg agccattttt ctagctcaag 7080 tgcctttgtt ctttttctgg agtttctgga tctagacagt gtgccttaat tttgttcttc 7140 agtgatgttt ccagcctgca accgagcttc ccttcctcgg ctggaaaccc atctttgcct 7200 tctagattcc tgaagaggtt gaatttcatg tgctgactac aggtcccttc ttcaaaaccc 7260 attctgccct cagaatctat gaacccgatt taaaaaaaaa aaggattttt atttttatgg 7320 gtttggctat tttgcctgta ttcatatctg tgcaccacat gcatgaagtg cttgcagagg 7380 ccaaaagagg gcgccagata tcccggaact ggagttacag atggttgtgg gccagtgctc 7440 tggaaaaaca gtcagtgctt ttaaccccta agctatctca ccagccctca tcttctctta 7500 gaccgagtgc tagggctcaa actcagggct tcatgctctt gcagcaggct ttgtaccaac 7560 ggcactgtct cctaggctcc cttctatgtt tttgtttatt ttatgccgaa cacatgtgac 7620 atagtataca tgtggaggtc agaggtcaac ttcaaagaat ctactccctc cttttatcac 7680 gtgggtccta gggattgaga agtcatctgt gatttttgac ccagctgccc tacctcattt 7740 tgcagggaac agggatgggg atgggaatgg caaagggcag gacactgttc caccttccgc 7800 ctcaaggctg ttaacaaggt ggtttaatac tgaattgcca gcccagccca gcccagcctt 7860 tcactatttt cccggtattg tcaaacattt cctccacact acctatagaa tattcttact 7920 tatgtttaat gatctacttt agtgtttatt ttaagtacat actatgttcc gttccttgac 7980 ctcgctgtac tttgtttatt cgtaaatata cttacccacc tgttgtaata aaaatgtcct 8040 tctgtccttc cttcctttct tcctttcttc ctttcttcct tccttccttc cttccttcct 8100 tccttccttc cttccttcct tccttctttt ctcccttcct tctttcctcc cctccttcct 8160 tctttcctcc cttctttctt tcctcccttc cctccctcat ttctttaacg ttcaaccaat 8220 gtttctttct cacgtttcct ttccaaggtc agttccgagg gctggacaac cacactaagc 8280 tcagcggctc cgcacatcct gccctcgtgg agcccatgtt aaagagaggt tggtggagac 8340 acgcaatgga cactggagac aacgacttag cttagtaccc taaaagtgaa tggaggatat 8400 gcaaaaataa gatagagggg agagcaaagg aatttggcgt ccagcgttag tgtgtgtgta 8460 ggagtgcgta gtaaagagca gaagctcgtt acgtgtgcag cggatgtgag agtctcacag 8520 agggaacccc cagcgctaag ttcctgaggc ttggtgggtc aaagaacaaa ttaacctcag 8580 catagttacc cttccataag caacaaattc tccaggtcca ctaagcctcg tgatcagcag 8640 cctgtacagt cacttatgag gaacttttat agggagaaca ggatgtgatg gtgtatttaa 8700 acatatgtat ttaaagatgg ctcagtgaat aagggtgctt gttgcccaga cctgagttca 8760 atccttgggc tccacgtggg ggaaggagag aaccaactcc tgatcctcca catgaatgct 8820 gacatgagca tgcacataag caatacatga gtctaatttt aaaaaaggaa aaatacctct 8880 ggttaacata taataaattg ctattctttt agatggttgg attcctaata cacaccagca 8940 cagagtgaac gcactgggta atgtacaata gcaaggtgac ttacatcaaa ctacattaaa 9000 tgtttaaaac agtgaaaacc tgaaaagcta acatttttgt ccaatgtgaa aaaaaagtct 9060 tatttagtag catgatatac acagaggtaa aaatagatat cagaaagtcc cccacatatg 9120 ctaatcaatt ttgttcttaa tctttacata tccacttatt tttgtgatat ctttatttta 9180 tgcataaggg tgttttgcct gaatgtatgc ctttgtaaat acttgcatgc agtacccaca 9240 aaggccagaa gagggcgtga gatcccctgg aattgaagct actgatggtt gtgaaccacc 9300 acgttggtgt tggaaatctg acccaaatgc tctgcaagag tcgccagtgc tctgaaccat 9360 gtccaagtta tctctccagc attatatatc cacgtttgaa gtagaggtac attgggcttt 9420 tcatttttat agtggttact taaagcatag tctatgctgt ttttaagtga cagaatgttg 9480 ttcattgtat ttaaaaaata tagacaaaaa tttaaacact ttaaaaaata ttatttttct 9540 ttatgaagat acatgctttt aaggggttct gttggagaat ttcagtagcg ccaacttaaa 9600 acaaatactt tattagggct gtagaaatga cttagaggtt aatagtgtgc actgctcttg 9660 cagaggatcc gagtttggtc cctagcaccc atgtcagggg cttacaacac aacctcctgt 9720 aactgatgct gcaggggatc ccaccccctc ttctggcccc tctcatgcac aacacacatg 9780 aggcaggcac agactgatgc acatatatgt gaattaaacc ccaaataaaa tcgttttcaa 9840 aagtccaatg aatgtcttga caatgctttt ataatcaatt cattcatgac aggtggttcc 9900 ctgtgtttgt gatttctctt caagctgcgc atcaaagcaa cccctccttc tccctccttc 9960 cctccctcct cctcctcctc ttcctcgttc tcctccctct ctctgtgcat gtgtgcctgc 10020 ttacctgttt gcgcaccgca ggcgtgcagg tgcccacgga gtgattaatg aattctttgg 10080 aactgggtta caggcagttg tgggcgatgt agtgtaaatg ctgggaacca aactcgggtc 10140 ccttggaaga gcagcagtgc tcttcacaga tgagccatcc ctcaagtccc caagaacttg 10200 tttctcttgc tcagcttgca ctctccaaga cttaggaaag tgcttagtga ataagctggg 10260 gcacaaaaga tgcggttata agaaggtgac gtaggagcct ctggcaagcg atgcagatgc 10320 caattctcac ttgtgttccg gtccctctcg cttttgtcag ttttagaatg ttctaggctg 10380 ggtttggtgc tgtcgtagca cagagaggaa acagcatgaa ggacaaccag gccagagata 10440 gttgatggtc tgagcctttt gtttgctggc ttcactcgct ccgctgttct aaagcttcag 10500 tcaatgggtc tatgttgaac cagtttccgc tgttcactac aaagatgcaa ttggaagccg 10560 agggagggag ggagaagctc tggagagtat ggctcagtgg ttaagagcac tgactgctct 10620 tccaaaggtc ctgagttcaa atcccagcag ccacatggtg gctcacaacc atctgtcatg 10680 ggatctgact ccctctgaag acagctacag tgtacttaga tataataata aataaatctt 10740 agaaaacaag tagctgaggg aggagttttt tacttgtctc atccagggtg cctggaggat 10800 gacggcgagg cgccactttg cttacctcac caagtgagag ggacaaaaca tagttttgat 10860 ctgcagtagc ttcgcggtga gcaccacatc tccacctagt cctccgctat tgtcggctgc 10920 tatgaacaga ccctgctgtc acctggccat ttatctccat ggtcctgttt aagctctagc 10980 ctattgacta aggggatgca agttgaagtg gatgtcccca cgtttgaaca cagcactaca 11040 tgctggtatc tgaaatctga gtcacatcta ggtcagtcac attttctaga tttatgagtc 11100 tagattttat ttttccaggt gtcttgtttt tctgtctcta aatgatgaca tccacccccc 11160 tgccttcccc ccccccccca cacaccatat accctgggta tggttatttg tagcaaaatc 11220 aatactcttg tacttgaata atttttttct ttgcattttg aaccaaggtg tttattcaca 11280 aaaggaagaa ttgtccattt gtctgtctgc tcagtctttg gcttagcata gggcatgtgc 11340 agaggctcaa taaaaatctt ttgcctcaat cagtacatgg cacatgcatt acctcagggt 11400 catatttgta aataggtatg ggtgtttgcc tacaggtgtc tgtgcaccac ttgcatgcag 11460 gtgcctgagg aggccagtag agggcatcag atctctggga actggagtta cagatggctg 11520 tgagtcacca tatgggtatt gagtacaaga gagactctgg aagagcagac agtgctctta 11580 gccactgagt catcactgta gcccgcatgc catattatta aggaggctga acaccaagtg 11640 attcagcctc gctacagaca agtagaaacc tgtctctggc ctccttaacc acaaagttgg 11700 ggggcttccc tcgacacaga agccagagaa ccatttgaca ccgttcctct gtcacaggct 11760 gtatcattgg tccattttcc acatgacact gtgcattcaa gcattcatct ctgcagatcc 11820 ctgggcagta tggcagtgtg tgtgtgtgtg tgtgtttgtg tgtgtgtgtg tgcttgtgtg 11880 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtttgtg tttgtgtgtg tgttctaatt 11940 ggaaaggaaa tcttcacttg cctttaaagt gcagtgctac tatatttctt tgcaaatata 12000 tatacgtata tgcatatgta tatgcagatg tatacacaca cacacacaca cacacacaca 12060 cacacacata tctcaaagtt cctttcaggc tgaagaatcc attattgtcg ggccagtgag 12120 atgacagagc agtttaaggt atctgctgcc aagtctgatg acctgagctc aatccctgaa 12180 atccacaccg tggaagggga aaactgtctc ccacgagttg ttttcttgac cgccacaggt 12240 gtatgcaaat atgcatgtgc acgtgcacac aagcactaaa taaacagaaa gtttaaaaaa 12300 ttaaggtata tattatttac tatgtaagag aacatcaaaa aagtcttgag aagtacacca 12360 tgggagaaaa cacacctagc ccttcaaata atttgccctt ctagaaaaat cctgaaatag 12420 ccctttcccc atacagtagt agaatgataa cattatctca gagatgagca ggcgtttaag 12480 gctcagcgga acgggaagag agttgggttt ttcttcctat ttgttttgaa tagctctgct 12540 cccgggtcct ctgcttgccc agatgatctg tgaaatgggt gagtgagcag cgcacctccc 12600 cagcgcgact gttactgacg tcagcaagcc tcattagctg ctgtttgtct aaccaaaggg 12660 aacagcgggt ggagagaatc ttaatgacct gacttcccac actgcaggtg ccacttgcct 12720 ggtgtgcacg ctgagattgc atcagctgac taggcaaaca aggaacagtg gaaatgggag 12780 gcagagaaaa agcccaagag agactgccca gtgagtggaa ctcgctgctc catcctggga 12840 gagtgtagca gagatgtttg tgcgtggtgg agtgaggtcg actcaggttc acagctgtgc 12900 gctgctacat agtagttgag ggtcgaatca cttaaaggct ttagtcccgt tttcaaaatt 12960 aaaataggga taatgataga accttaggga gaaggaggga cacgtgtgta aatcacagac 13020 ctcattgtac cactggataa ctggcaggag ccactgtttc tgactctaca gtggagagaa 13080 gggaagagca atgattggca ctgtggacgc aggtggcaga gaacagctaa agatgacttt 13140 atatcctaaa ggcaagtggg gtagctgtga gttttaaaga ccgaaccttg cttcacaaaa 13200 ttgctcttgc tttcctacct accaattctg cgttctttct ttttccctgg cagcatctcc 13260 ccggcggact cagccttggc tacagcggag actcctggga aggaaatgtc cttccccagc 13320 cctaggcaca gtggttattg tctgttctca catcctgtga gccctgtagg cagaggaatg 13380 ccccagaacc acagcaactg tgcaaacatt tgaattccca cgggtagttt cccatcctcg 13440 tacccatgag gcttctgctc tctgtagtca gaagaacgta cacagttctc aggagcagga 13500 agtagagggc taggaatcat gggtgaggag cctagcatgt gtgtgaatac atccgtggaa 13560 aacgtttttg agggagatag tacagaatcc tactccaggt gaaataatga gactcttgct 13620 aagaccacgt acggatgacc caatcaatac acaaggaggg agttgaaaag gagaaaaggg 13680 gttggccatt aatttagcca acgtataatt ccacgtatta gcaagtaact ttaattatcc 13740 tatgcgccct cggttttatt ctccacagct gtaaatggta ataatacccc ctttgaacat 13800 ttatcatgat taattaaggc agtgttgttg aaacatattg aaagttacct aggctaactt 13860 tgtccaattt gccagctgct ttggcaaatc ctctgcactg gggctggctc aggctagaac 13920 tatcttccat ctgggtaaat ctcttctcca ttccagagca gatatcatgg cgggtagctt 13980 gacctcacag tcatgagaca actcaaacca tggggattgg caaatgaagc aagtccttgt 14040 tattgaactc tccactcaga aaaaataaaa aataaaaaca tattaatact acggaggatt 14100 ccagtgacac tcccttctgg gaaagacact caagagattt ttatgagcag tcagggcaga 14160 tggaagtcac aaggcaaaca cctcaaagta aaaaaaaaaa aaaaaaagat caacatacca 14220 gagcactcaa caagctgaca gtctggctgc ccatgctaga tgcttagaat acatgaatga 14280 caaagtttgt atgcagtctc tgcctttagg gactttactg ctaatggggg attgtttatc 14340 aaacctaaat aatatccttg agggaatatt actatactgt gggtattagt gttcttaacc 14400 aatatgggtg ttgactttgt ctgtgtctct ctctgtgtgt gtacaacgtt tgggagtcaa 14460 ttctgtcttt ccaccacatg ggtcctggct atcaaactca gtctctcagc attactggaa 14520 gatgcctttg ccccatgagt catcttgaca gcaaaattgg gtattgagaa cttgaaagtt 14580 tgctgtgtga agaataagta aaacatgcta aaaaaaaaat aaagtagaca ggctgcacag 14640 atggctcagc agttaagagc actggctgtt cttccagagg acccaggttc atttcccagc 14700 acctacatgg cagctcacaa ctgtctgcaa cttctgttcc aggggatcca actccttcac 14760 acatgcaggc aaaaacacca atgtacatac aataaaaata aataagttat ataaaaatag 14820 gcaatctcat agttttacca tgttaattat atgttaaact agcaatgtgc atatattgca 14880 ttaaataaaa atgattcctt aactaaatta atttctttaa ttaaataaaa tgcaaaaaaa 14940 tgaatgaaat aaaatcactt tcacaaggtg gagaaacact ttgggaagac atccagatgt 15000 gaacctcagg cctccacata cacaaacatt aataagtatg ttaattaaaa ttaatttcac 15060 tttactttta aaaatagaac tactagattg tttaggatag aatatataac ttctgcccaa 15120 tatttgctca tattcttttg gaaaatatgt gttcaaatcc tttgcttatt cttaaattag 15180 attgcttgct tttattgttg tatttttttc tatcccaaat agccaaatag tatatttgtt 15240 tggaggtgta tgtggctgtt ttaagacagg atttcttttt ctttttcttt tttttttttt 15300 tagctctggt tatcctggaa ctcactctat agggcagatt ggcctcaaat tcacagagat 15360 ctgcttgctt ctgcctcctg catgctaggg ttaaaggatt gcactggtgc tgggcatgac 15420 aacccccccc cccctttttt tttaaccttc actggtatag tacaaatatg gcacacagac 15480 cattcaccca tacacataag agaaggacta aacaaatatg tttaaacatt aaaaaaaaaa 15540 aaactattgg aaacggaatc gtcaaactgg aatggtggag atttcatcag aactggcttt 15600 gttcattttc ctgtctccgt caaactgtct gctctgtcac agtctggttc tccaccacgt 15660 ggctgctgct tctgttggtt cagatccctg ctccgcagtc tgagccaatg aaatcccggc 15720 tcttcacaaa ggcggtttta gtggcaagtc tttggggctt cttctgttct cttggaagaa 15780 tggccccagc agcttcttcc atagcgcttc tggtgaacca gatggcatct tgttcaacct 15840 cttactgcac tgagcacgca gcctcccctt acaagattca taccaatgtc tcattgtttt 15900 ccaaagtaac ttgagacttg aactttccca gactctcaaa taaaaccatt ccttgtggaa 15960 gagtgcggtg gtctccagtc tttagagact gcctgcctac ctaagcttta ggggacagag 16020 tgggacaaca ctaacaaggt gtgtgggcat gacaacctct aatcttctca gctagcttca 16080 cctggaatct cagtcttatg agtaatctgg actaacagta agggactcat tatttgatgc 16140 ttgccacatc tggggtcaaa tcttggcccc tagaaatggg agatggatgg acaaagatgt 16200 cctatacctt tagctatacc ttctaagaat tcagcctcaa cagcttggag acagacggga 16260 ggacaaacag ggtgggcttc cctggttgct gggcccgctt agggcttggc tgtaggctga 16320 aagggaaggg agcttgctct ctcggccaca cactgtactt gcatctccat cgctgggatg 16380 cagacagtga agagaacaga ccgtagctct gcttgttctg agacatagga aagcgctcca 16440 acaaatgttt cttcacctat cgtatgctct gaggacaatt tccacagact ctagatgatc 16500 atcgttgcac aatttttagg aatcttcgca gtttccttga agtgtgtcct caagagttcc 16560 tgatgctgcc gttatagaag tgggagtagt tgggggtttt atagacaaac tctaaaacta 16620 aaagaaggag atatacacag ttacagaagt gtttggtcag caaggagtca aaaaattagc 16680 cagtgcacac taggcagcat attaaagtat ataatatagt atatattata tactatatat 16740 attataaagt aatgtattac aaagtaatat attactatat attataaagt aatatactat 16800 aattaatata taagtaatat actataacac atataatata taacaaatat aacatataac 16860 atgatatatt atattatatt atagtatagt atagtatagt atatataata agatactact 16920 ggctactaat ccatccaaat ctagaatgaa ccctgtgtaa acacacagga aaacagtggg 16980 aaatctagta gaaaactgga caataggtat taataggaaa ttcacagaag gcaaaacttt 17040 tcaaaatgac aagttcatga atggggcttt atcactatta aatcctttaa aatgcattta 17100 aatgccaagg cgatggctca gctgttaaag agcttgtggg gggatcagga ggaccagaga 17160 ttggatccta gtatctgggc caatgtcaga aggatgtggc agcctgccta taatgctagc 17220 tttggaaggt ggagtcaggg tatccccaga acaagctggc tcgtgggatt agccacattg 17280 gtaagctctg ggtttaattg atagagcctg aatcaatgaa gggaggagag agtgacacaa 17340 agagactcct gatgtcaacc tcaggactct cttcatgtat gtggacacat gtgcatgcac 17400 ccacacacac gtgtgtccac atatttgaaa acagatattt acatacaata cacacacaca 17460 cacacacaca cacacacaca cacacacatt caaagtaagt aagtgcataa aaatggttac 17520 attgtagcag tttgtatatg cttggcccaa gggaagtggc actattagga ggtgtggcct 17580 tgttggagga agtgcatcac tgtgggggtg ggctttgagg ttcttttttc cattttctct 17640 atttgatttg attactacag ccatgaaatt atgatgatct acatatacag attttatgac 17700 aagaaaagaa aaattcataa aaattttgaa gctggagcag tgattctgtt ggtaagatgc 17760 ttgtaagctc tgggctcata tccacttatc agtgagtgca tatcatgtgt gtccttttgt 17820 gattgggtta cctcactcag gatgatgcct tccaggtcca tccatttgcc taagaaattt 17880 cataaattca ttatttttaa tggctgagta gtactccatt gtgtaaatgt accatatttt 17940 ctgtatccat tcctctgttg aaggctttga ggttctatgt ttaagctcta ctaagtgcag 18000 aagaaagctc tcctggttac ctttggatca aaatgtagac ctctcagctc cttctctagt 18060 accatgtctg cctgcatgcc gccatgcttc ctgccatgat gataatggac tgaacctctg 18120 aacctgtaag ccagtctcaa tgaaatgttg tccttcataa gagttgcctt ggtcatggag 18180 tctcttcaca gcagtaaaac ccaacaaata tcactaagca atttttaaaa ataaagctaa 18240 gggctagtga gacggcttgg cggttaggaa tgcccgctgc caagcctgaa gacttgatga 18300 gtttagtttc cagaatttat atggtggaag gagagaacaa actcttagaa ctgtcttctg 18360 accttaacac acactgtgtg ctacactcac gcctacacaa atgcatgccc acgtaaacaa 18420 atagatgcga aagtcaatgt aaatacaatt ttttaaaaaa aaatgaataa catgcagtta 18480 agatgaggtc atattatact ctcaccattg gacaaaactt atatgttatt gtcctcattt 18540 ggttctgttg ctgggataaa atgcagacca gaactaacct gtggatgaag gggttcattc 18600 tgtttatagt ttatcctcct tcactgaggg aaggaaaggt ctacatcacc cagaggaaga 18660 actgaatgaa gcaaagacca tggaggaatg atgcttactg gcttgccccc aggctcaggg 18720 tcaacgacct ttctcatgcc tcccatagcc acctgcccag ggatggccca gctcacaatg 18780 gcctgggtcc tgtcacatca atcaacaatc aagaaccttg cctcacagac atgcccacag 18840 gccatccctc aaccaagatc tctccctccc acatgactac agtttgtgtg aagttggtga 18900 aaaccagcta gcagtttatc aaatcttggt gggaaaaaga tagagagaca gaactacgct 18960 ttgaggagca acttggtggt actaagtgaa attaagtata tataattatg gctaaggaaa 19020 taacattctt gggttaatac cttagaacat cttccataaa caaactagag gaaggtaatt 19080 taaagcacaa ataggcaact aataccatct taaactaacc tacacaccta cgggttatga 19140 atggtgacca caggctctgt atgtctacta agaaataact atgtgagtca aaatgaaaga 19200 accaatcgat ctacatacgc cagcaaggac ggtttctaac tgtgcttcgg atcgtaaaga 19260 taacaaagca aaattagatg atagcatatt aaaggttatg taacatgtga atataatggt 19320 ttctgtggtt cattgttaca tccgtttatg aatgaagctg tggttggtga atggtatgca 19380 ttaggataat aagcgtgggt acctggtaag agaagagatg aaaatggctg gaggaggcag 19440 tgattacagt tccattcaga agggaatcta gagcctggga gatggcttgg taaagggctt 19500 gccaagcaag catgaggatc gaagttcaaa tccccagtcc ccgtgtaaaa agctaggcag 19560 agcaacacat gctcagagcc gaggagtcag agacaggcag atctctggag tgcactagtc 19620 agctgactca gctgagtccc tgcactccag gtttagggag acactgtctc aaacaaacaa 19680 acaaacaaac aaacaaaaca gctggaatgt atgctgactt agttttactt gacatagcta 19740 gagtcacggg agaagaggaa acccccaatt gtgaaaatgc ccccactaga ctgacccatg 19800 ggaaagcctg tagtgaattt tcttagtgac tgctgtgaga aggcccagcc cagcatgctg 19860 tgagtgggtt gtcctgggtg ctgtaagaaa gcagactgaa caagcatcgg gaggcaaaag 19920 ctgataagtt gtcccttcct gtctccaggt tccttccctg atgtccttca ctgatggagt 19980 gtatctgaga gttgtaggct gaagtcaacc ctttcctccc caagtagaca ggaggttaga 20040 aagatagccc tgtgggtcag agtgggtgcg gtacaaatag gaggatgcac cttcatatct 20100 caacctttgc atgagcatct gggtgtagac acacttatgc ctgtgacacc tccttggagg 20160 tggtgatcgt gggactgact ggccaccagc ttagtttcag gttcaataag ctaacctgtc 20220 tcagggaagt aagatgagga gtgagagagt ctggaacacc ttctctggca tttgcacgca 20280 catatggata cctctccaca tgcttataca gtacatacat acactccact ggcatgcaca 20340 cgtacacatg cacaccaaca acaaaaaagt aagttgaaaa aaattaaaga aacactccat 20400 gttaacttat ggcctttaca tgcatacata tatatgtgca cacgcacctg catacactta 20460 cacagaggga gagattacat aggcgttgtc agcagtgtgc tgcagacaaa gaggtatgat 20520 cagtctttct gagatgaatc ttagtgatga ggattccatg atgtatgagc aacagggctg 20580 ggcagtgcag ccagctggtg ctgcatctcc tttcagtttg gattatctta ttgggtcaca 20640 ccagaaccag ggagataaat agtcaagaca gactttgaaa attcatacac cacacacaca 20700 cacacacaca cacacacaca cacacacaca cacacaccaa taaagctagg tttgaatgaa 20760 ttacttttgt aaaatccagg tctgagttgt attctgaaag ctttacttgt ttggactggc 20820 cagtagctca gtcaggcaaa aagacaccac acgggagggc agactttaaa gaagaacgtt 20880 tcaatgaggc aagctgtcac ggcttgtgtg aattacacaa tgaaagggaa aataaaataa 20940 aactatggcg ggctaaaatc tgtaagttct gacatgtggt cctcgtggca ccatctgctc 21000 atgaaagcat gaagaggctt ctggaacttt ccaggactgg gttgatttgt ctccacaaag 21060 ctgcctgggt aagaccctgt ggcactgggt catcaagaag gtactagcag acttcctttt 21120 tatctcagta tttttctacg tcagtcccca aaccatgaaa caacagggct taccctgcca 21180 caccaaaggt caaactatgt tttctcttac ccaactattg cctgggatgg ctccaacttt 21240 attactaccc cacctccaac ccgtaataac tctccagctc tcacttcaaa tccaagagag 21300 tgtggactga gtgaggggac cagagacagg tgcatctggt agaaagttat actcaattaa 21360 ctaactgaat gcatttgcat gtggttttaa ttccactgtg cagcaatttc ctgagtaaaa 21420 atggagagat tatatctagt ttttgagtca ctgtaagaat taagtaaacg tagctctcaa 21480 tctatatctg cacattattt ttcctcgtgg tcctagattt ctcttgttcc tgaccatttg 21540 tcccgtcttg gaatttcccc agccttcaga ggtgtagccc tataatttac cgtccagatt 21600 gcaatccctc ttcatggcat gaattaagtc ttatctcacc aggaagtgct ctttcctttc 21660 ttctgactcc tctccctctg gctttcctct tcatttcttc tctacactgt ttagggaaag 21720 aggtttctta gcaaatcctc agacacccca actagtgggc ttcccggaaa gtctcagccg 21780 tgtgtgggag gtctgccctg aagaagttct ttgccagaaa atgaagcaag gtgttctggc 21840 tggacagtga gggtggtaga ctgtaataag tatgtctggc tttaactctt ctatattcat 21900 ctgaggggag catctgagcc caacacaaca tctccatcgt caatggagaa tcctactcag 21960 ctgacaaggc ccttgcttct gtgttgaagg attacagcaa tggcctcctg tttattgacc 22020 tcatacttag acccatgagg catgcatatc atagtttatt tctcctactt agcaactgaa 22080 aatactaaag ggagtcacag agtctgactg ctctcacggt ctgtatctat tgcttaatcg 22140 aaagcttccc tgttaccctc ccatagcaaa taagggtcca tcccacaaga cactgtggaa 22200 acgcctcctc tcaagctcct ccagacctcg cagtgagtca atagagagca gaggggtctt 22260 acgtctctgg tgcctaaaga ggatggggtg ggcgttaggt ctcagcaaga acatctcttc 22320 ttatgtggcc agcggagagc ccagaaatct taagcttttt cataagggtt tctttgagac 22380 gcttcctaac ctagtgatcc ggttatcacc aagctaaggg aaaacgatca cgtgacacga 22440 aggatgaagg aaaaccctca gattcctcct cctctgaatc tgggagtgga aaactgagga 22500 gctgagctca gagttccaga gccacgcgaa gctgggctca gcagtgtttg cagctgcagg 22560 tctggtgagc ctgtggaaag agagaagggt gagagaagag agtctccaaa aagctgcccg 22620 aattagagaa acaagagacc ctgcctcaaa aaggcagaca cctcaagtgg tcctctgact 22680 tctgttcatg tgtcatgaca cgagcatccc tgaatccaca tacacagctg agaagcgtgt 22740 acacacacac acacacacac acacacacac acacacacag agagagagag agagagagag 22800 agagagacag agacagagac agagagacag agagacagac aaagagacaa agagacaaag 22860 agagagaaac agagagtggc tgaagggagg gggcagggga aggagggagg gggaggggga 22920 gagacaaata atttataaaa ggaaaatcat ttaaaacatt tcagggctgc cgagatgacg 22980 cagttgcaag caggaagccc tgagtttgga gccccagaac gcatccaaaa gccaggtgtg 23040 gagcgtgtgt gttctgtatc tcagctctgg aaacaagggc ctcgccatct attgccaatg 23100 ggctgccaaa ccaacaagga gcgagcaagc cccacttttc acgtaaaata cctcaaagag 23160 aaaagcaaca gatgcaaagg ggagagaatc tcgcttttgc ccagatcatg tagcagcccc 23220 gctcttagag gacgtactcc atgtctatgt gacttcaact ttcaattcta caaaaagggt 23280 tggtttcatc cctctcttct caatctggaa acagttcgtt ttaacgacag atgggtgata 23340 ttacacttag aatcaaactg ttaagccatg tgaatgaatt tatggtcacg tcatttcgaa 23400 gcgtcccctc tacaaccccc ctccccccgc tgggaaaaga ggggcttccc gctttcccag 23460 gaagggaagc tgtgggctct gctgggggac acacactgag ggactttcat cagcatccct 23520 cccaagaggg aagggacatg tctgtgggcc ttatggtctt caaagggacg taaagccgtt 23580 gcattctgat tagtttgtgg agggtacttg tttcaatcca gctacagttt ggtttgtgca 23640 gctggtatct gctgagcagc tgccgggacc tgtgtactct cgaaatcggt gaggctaacc 23700 cctgagaagc agagggtcgg gtgtcacgag agacttttct ttcccttttt ggcagccata 23760 ttgaaagatt cacagacacg ctcttaactt atccatgcaa agtgagactc tgctcacatt 23820 ccctgatact ttccttcaaa atgcaccagg aaatgtcagg aaataattca attagtctca 23880 ggaaatttac aaactgtgca aaacgttttg aatccacttt tagaacactt tcaatgacca 23940 caaaagctga agaaagggca gaaagaggca agggaaagag gaaaggaaga aaggaaacaa 24000 acaagcaaac aaatccagtt tgtcttcaga aggattatgt tggaggttcg ttttgcttgt 24060 agagactagt tcatctattc caggcttgct ttgaattccc tgtataatgg agacaggccc 24120 tcaacttctg gtccttcggc ttctgtgtgg cattacagtt gagtgctatc acacttaatt 24180 atgtcagagg cgtttttgtt tgtttgtttg tgtttatgtt ttactttagc agtggctgga 24240 gttggggtgg ggttgggtta ctccagcagc tcccaagagg atcagccttt caggctgtcg 24300 ctggaagggc agctatatag atgctgcaag aaaagcaact agctcctgct cttagagaac 24360 ttttgtttat tattattatt aatttacttt atttacattt aaaatgttag cccctttcct 24420 ggtttcccct tcgaagaccc cctatgccct ctcccctccc cctgatcacc aacccaccca 24480 cacctgcttc ctggccctgg cattccccta cactggggcg ctgagccttc actggaacaa 24540 gggcctctcc tcccattgat gactgactag accatcctct gctacatatg cagctggagc 24600 catgagtccc accatgtgta ctctttggtt ggtgatttag tccctgtctg ggggtactgg 24660 ttagctcata ttgtttttcc tcctatgggg ctgcaaatct cttcagctcc ttgggtactt 24720 tctctagctg cttcattggg gaccctgtgc tcagcccaat ggtaggcttc gaggaaccac 24780 ctctgtattt gtcaggcact ggtggagcat ctcaggagac agctatataa ggctcctgtc 24840 agtaaggtct tgtactcagc tattaaaaag aatacattta tgaaattctt aagcaaatgg 24900 atgaaactag aaaatattat cttgagtgag gtaacccaac cacaaaagaa cacacaaata 24960 cgtggtatgc actcactgat aagtggatat taggccagaa gcttggaata cccaagatac 25020 cattcacaaa ccacatgaaa ctcaagaaga aggaagacca aagtgtggat tctttgatcc 25080 ttagaagggg gaacaaaata cccatgaaag gagttacaga gacaacgtgt ggaacagata 25140 ctgaaggaat gaccatccag agactgcccc acctggggat ccatcccata tacagtcacc 25200 aaacccagac acttttgtgg atgccaacaa gtgcttatgg aaccttttat ctatagagaa 25260 ggtttctcag ctttggtagc attaattaat tcgttgtcat ggaaaggtgc cgtttacagg 25320 catcccttcg cctctccctg tagttgccag aaacatctta gatatggatc aatatcttct 25380 ggggccagga gatattccca aattgagaac cattggtcag cactctgtct atcatgactg 25440 caaacaaatt gaaaacttga caaacattaa tgaaaacttg ataaacatta atgaaaagaa 25500 actcagaaaa cactttgata gtattttctt taagacatgc catgcgtggt ggcgcatgcc 25560 tttaatccca gcactcggga ggcagaggca ggcggatttc tgagtttgag gccagcctgg 25620 tctacagagt gagttccagg acagccagag ctacacagag aaaccctgtc tcaaaaaaaa 25680 aaaaaaaaaa agacagggtc tcatgaaacc cagactggcc ttgaactcac tatgtagctg 25740 tactgcctgg gtttgtgtgt caacttgaca caagctggag ttatcacaga gaaaggtgcc 25800 ttccttgagg aaatgcctcc atgagatcta gctgtaaggc atttttttca attagtgatc 25860 aagggtggga gggcccattg tgggtggtgc tggactaacc tgggctggta gtcctggatt 25920 ctgtaagaaa gcaagttgag caagccaggg gaagcaagcc agtaagtaac acccctccat 25980 ggcctctgca tcagctcctg cttcctgacc tgcttgagtt ccagtcctga cttcctttgg 26040 tgatcaacag cagtatggaa gtgtaagctg aataaacccc ttcctcccca atttgcttct 26100 tggttatgat gtttgtgcag gaatagataa cctgactaag acagtagcca acgatgatgc 26160 tgagcctctg attctcctgc ctctaccttc tgagtactgg atttacataa gtgagctgtc 26220 acaccagttt atatggtgct ggaatcaaac cacaaaggct cccagcatgc aaggcaagca 26280 ttctctcaac tgagctacag cctcagtttt agcaatgcaa agaagaggaa aaggagggag 26340 aagatatata tatttttcat tacatatatc tcacatatat tttacattac aaatatctca 26400 catatatttt aaagcgctgt agtggtgaga tattgggctt tgtagacacc aggcattctg 26460 ctgtcctggt tcttcaggct ttatgatctt gaacaccata gttctatttg gtgagtctgt 26520 tttctcagga gttgttgact atgcctttag tccagtgcta tctgaagaat gaagatagta 26580 tctctctcag tacacctttg gcaagggaag ataccatctg cccagtatca tcatgtacat 26640 tggttgagag ttaactctgt gctaagtgtt gtggtaaatg cattatttct tattccatcc 26700 tcatggcaat cctaccaaat gtcaaaaatt attatccaca atttgtagat aagaaaacca 26760 aaactttgac acattaaatg actgtcttag gattttgttg ctgggaagag acaccatgac 26820 caaccaaggc aactgttaca aagggtaaac atttaattgt ttctctgagc acttctgtct 26880 tccaggattc taccacaatg gcctttctgc tctatttaca gtattcaact gtttttttag 26940 tacaaagttc caaggtcgtc cacagtcctg caaaaagcaa cacaatcagg tacttcccag 27000 caacggcgat tcttgagtat cgatttctgt attatttaat cttctgttgc tgtgataaaa 27060 tgccacgact aaggcaattt acagcagtaa gagtttattt gggcttacat ttgcagaggg 27120 ataagcgtct ctcatggtag ggagattgga aggcagtgag tggggcctga cagctggaat 27180 aggaagcaga gtgatcacat cctttactgt gaacaggaag cagagacagc aaacagaaaa 27240 tgggctcaag ctaagaaaag aactctcaag tccagccgcc agggatgcgc ttccttcagc 27300 aaagatgcta gctctccaac agcatccacc atctggggaa ccaagtgttc aaacacacgg 27360 gtctgtgagg aatatgcttg accttaacaa agctagggaa atgtgtagac tcctctacac 27420 agctggaaga tgttcactct ctcttctttc ctttaaacag tttcttaaaa attatataac 27480 ccactcatat tttcctaggt gttacaatca taccttacct ttaatcacat tttcatttat 27540 ttgtttgttt gagattttat gtttaattat gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 27600 gtgtgtctgt gactttgtgc acatgaatgt ggtgcccatg ggagccacaa gaaggcatca 27660 gatcatttgg agctgggaaa gcagagccgg aggcagggac atctggcaga gttcagagtg 27720 gacatgacta gactgagctg ggtcatgtgg ggagatgtgc agaggaaggg aaaggggaga 27780 gatgggaaca aggtgcggca ggcaagagag caaagataca cacacacaca cacacacaca 27840 cacacacaca cacaccacat atgtacatat acacatacac acatgcacgc acgcacacac 27900 acacatattt agatgcattg aaatcatttg atatacaaat agaccatgca aagatcaagt 27960 cagtgtgatg gacatttcca gctgtttaaa ctttttaata cttgttagta ttgagagtcc 28020 cacccccacc ccatttcact gctttacaaa gatacaaaac attgttctgg gcatgttggg 28080 tgcattcctt aatcctagtg atgcagaggc aggcagatct cggtaagttg aaggctatcc 28140 tggtctatac agtgcattcc acgctagcta gggctaccag tgagattgtt tcaaaaaaca 28200 aaagtctgta gttatcttcc tatattgttt attcttctct gccttcctct ttttctgcac 28260 acctgtcttc tctgacactg gtcagcatgt gtgagtgctt gggcccgtta atttgtgtga 28320 gcagacatgt gtcaacatca cttagaggaa aggtggcaga ctcattaaca cacacgatta 28380 acatgattag gaccagtttg aaagcagcag ttctccatct tcctaattca ggcctgactg 28440 tgtcaggact ctacactctc tacactgttt tctcagctaa cttgtccttc ctgtctctca 28500 cctcttcccc acactgacac acatgtatac acagcccccc caccacatac ctaccccccc 28560 cctacaccac agacttccag agagagggag gtggtgacta cgctgtatgt gtttaactgt 28620 tacctgtctc tccttagttc tgaatgaaat ggaatctaat cttaatcctt tcctgtcatt 28680 attagattca tggagtccta acggccagag tctctagatc ctgtcttatg aaattcaact 28740 ctcatacaaa attcaaagta tcagtttcag agaaagttat ttgaaaaaaa aaactgtgga 28800 gttgaaagca attgaggctt attctggtcc tcttactcag tgtgtgtgtg tgtgtgtttc 28860 tgtgtatgaa tgcatgtatg catggggtat acatgcatgt atgcatgtgt gttcatgttt 28920 ggtgtatgca tgtgtgtatg tgtgaggtat gtgtatgttt gtgaggtgtg tgtattgtgt 28980 gcatatgtga gcacatgtgt gtgtgtgtgt gtgtgtgtgt gtgtatgcat tcagagacag 29040 agtgtgctca gcttccagta gatcctcagc taggaggtgg tatcttgtga gactcttccc 29100 tctgcatgct gaactgttga ccggcttgac ctgtgaaggt cttgtgcagg caaccacagc 29160 tgctgagagt tcatgcatgc aatagtcttc atgtttattg tagttctccc taactctggt 29220 tcttaccatt tttctttcta gtctgtgatg ttctctggat cttgcaggac aggccataat 29280 gcagataccg agttttattg atgtcaatgg tccactgggc tagcagcctt actgactctg 29340 ggatagatgc tgttaaatca atgacagtgg ctttttaagt tacatccaga cgttaggcag 29400 gaatagataa ctcagatata acacgaaggc ttacccctgt gtttttgatt ttaaaaaatt 29460 gtgagtgtgt tatacgcagg catggttatg cacattgatt tgtgtggttg tgggatctta 29520 tgtcagaggg acttagatgt cttcctttat tactaccgat gttattcttt tgggacagga 29580 acctggactt catttctttt tgccaggaag tcagaaagcc ccagggatcc tcccatctct 29640 tccttcttaa tttctgggct tctgtaggca tgagcaaaac tatgcctggc ttgttaggtc 29700 ggcactggta tctgaacctt tgcttccatg gttgtacagc aagtgttctt aactcatgag 29760 gtcccgattt ttttgtttta cttaatgcta tgttgtactg tattatatta tgttttgctc 29820 tctctctctc tctctctctc tctctctctc tctctatata tatatatata tatatatata 29880 gactggcttg caccatggag tatgtgtcag tcaggagata ccttttggaa gacagtgcct 29940 ttaaaccatt gaaccatttt tctgcccatc acaaattctt tggactaaaa aataagagtt 30000 tctttttcct taacaggaca aaactttatt ctctgatatt taaaatataa gagaatattt 30060 caaaatttca aaaaccagtc ccttaagtta aatcaagtgc ctgctccttc acatctctct 30120 tctcttttga tgtaagcact tattgatcta aactcacttc ttgaaaactc ggagcatctc 30180 acaggcttcg ctatgttgcc tctctacttc catttgcctc aagataattt ttaatttctc 30240 ttttaactat ttcattagcc tattgattat tgaggaccat gtcaaatttc ctaggttttg 30300 tgcccccctc ctcaccggag actttgaaag aatgtatgtg ctgcaaatga caacttagtt 30360 tcttgaagtt tattaatttc tttatgcata tgtatatttt gcatttattt tcactgtttt 30420 gctcaatgtt tgtcattgtt ctagtttcct ttctgttgat aaaacactct gaaaagcaac 30480 tttgaagaga aaaggacttg tttagtttgc tcttccaggt cacagtccat tactgagaga 30540 agtcaaggta agaactctag gcaggatgcg ggagccgtga gggagtgttg tttgctggct 30600 gactctctgg aatgttcaaa ctagttttat tatatagttt gagcctgcta gtctagggat 30660 ggtgctgccc acagtgggct gggtcctccc acatcaatca atcatgacat tttctatgag 30720 acacggccac aggacaatat gatcaaagag atctctcaat tgaaaactga aaaaacttcc 30780 tcttcaggtg aatctacgtt gtgtcaaggt gacaacttgt ccagtagtac taagcagggc 30840 agtcatcttt aatcagagag atgacagtat atattccctt actgtatggg tttgtgttga 30900 gacacctata atggcagaca gattaagtct tcctgcatct gctcagagcc cctgtccaaa 30960 cagttccttc ttccctcttg tctctccttc ttcagacagc ctacacaatt cataagcagg 31020 agaaaacatc cataggatgt tttccagtta gtgttctaga atttcagtag tagaaaggat 31080 ataaaaatta cttagaaaca gggagtactc aatggcaaag tgcttgcctg gcatgcatga 31140 agccttgggt ttatcacccc cccccccact cttagtccat atttatcatc tttcatttaa 31200 ggaagcttaa aataccagag gactagagag atggcttatt ggtaaagagc acttgttgct 31260 cctgcagagg accagggttt ggtttccagc acccacaagg tggctcacaa ccatcagtaa 31320 ctctagtcca ggatgtttga catctttttc tggcctctgt gggtactagg cgcacacata 31380 gggcatatac atacatgcag gccaaacaca aaagtaaaaa taaaaaatat tgtctaaaag 31440 ctatctcaga cacagggaag tgcagagatt tatcctaaac cacactgttg gttaatgtga 31500 aagtcaagct aagataaata aataaataag taaattaatt aattaattaa taagtaatag 31560 agggttggag agatggctca gctatttagg gtgcctgcag ctaggatgca tgttttgttt 31620 ccaggggatt gggggcatga gagaaacctt acaacctgct acagggaatt ggacatgctc 31680 tcatggcctg tgaggatact tatacacaga agacattctc tctctctctc tctctctctc 31740 tctcactctc tctctctttc tctttctctt tctctttctc tttctctttc tctttctctt 31800 tctctttctc tttctctttc tctgagacaa acacacacac acacacacac acacacacac 31860 acacacaaat aaaaacaaat cttggcttat ctgtgcatcc tttaatttca tcactaggaa 31920 ggaagacaga tctttacaag ttcagggtca gcctgatcta caaatcatgt tttagatcaa 31980 caaaggttat gttatgagac cctatcctgt ctcagagaaa caaaacaaac aataacaacc 32040 gctcccccac tcttatggta aaatgaaaag ttactaaagg taggacaccc aagagcacac 32100 gggacgagga tttctaggca agcatctttg tgcagtgttg cacctattat tgaccagcat 32160 tgtttcagga aatgttaggc agaatcaccc cgctgttctc ggaagcaaaa cagaggtgtc 32220 acaccttctg attagactgg ccagcttgct tttcaaatca tctcttcttt atcatcaaag 32280 agagaaggaa acctcggaac aatgctgtct ctgcaatgct atccccactc gatcttatcc 32340 aaggctgccg tctttgttgc catagccacg gagacctgat gcgcaggtat ttgatcttgc 32400 tatgtgccac tgaagggtgg atattccatc ggagaccaaa gaacaggagg ttgttaagtg 32460 tgttgtaggg aatcagagcc atggagaagt tgtttgatct gagtccaggg ctgagcagga 32520 cattggtaca tcctggatga aagctttacg gtttgtacac agcttggaga agtttaagtc 32580 ttggaaaagc cgggcagatc ccatgtgtta actcctccat tgctgccagc ctttccaagc 32640 ctgtttcact taacaaatat ttattgagcg aggtcattac ctccaatttt cactacggag 32700 acttgaaaat taagacacgt actctgcctt tgaggacctt tgaggcacac agatgtaaac 32760 tggccattgg ataatatcat acatgacacg tctgagacac gtgaggggaa agtagaaaag 32820 ggtctatgtc tcatacagca aatgtataaa gttctgtagg taaagtgcca tttgacatgg 32880 ctcctaaaag tccccaaaga tagatttatt tatttattta tttatttatt tgcacatgaa 32940 taatcaactt gttgaaggac tgagagacca cagaaaaaca agcaggagga atagcgacca 33000 tccaggaaag aggcttgact gggcagaagg gtcaaactcc aggagacaga agacaatttg 33060 aattaagatt agccaatctc agagattcct tatgaatggc ttgatgataa ctcctcagtc 33120 ttagccagtg ggaagttttc taggaggaat tctgattggg cagatcttta gccacatgca 33180 atgcccgagg gtagaagatg tgtcttctta ttctgggcag ccacactgtc agctgaaaat 33240 agggtgtgtg ggttgtcatg tggacagtgg cagttcctat aatccatgcc tacctgtgct 33300 gtagctgcac tccttgactt acacacacac actctctctc tgtttctccc ttcctccccc 33360 ctctctccct cccttccctc tctccttttg cagaggtggg tattgaaccc ccgacctcgc 33420 acgttatgat agacaggcac gtatccctgg gcgctatgct gtactattaa cgatttgtat 33480 ccctttcctc tggagaggag atggcgggct tttcaaaaga aagaaagaaa tattcttact 33540 ctatctatag tacccaacag tgtctggtac acctacatac acagatatgc agggcccgtg 33600 gaaacacaac aattccactc ctgtttaggc aacttttcat gctctttttt tccctctgaa 33660 cttggcttca tacagttttt agtgttcaac cttagtcttg ttaaatccac tccaccatag 33720 ctcagcctaa tcatcggctt ttgaatggat ttacagaact agacacacat ttcctcctac 33780 agagcagacc ttacgtccca tcagaaagca gttggttaca cccaaatgtt gtatggccct 33840 ccattaaaaa agatgagctt atttgtttta tgtgtgtgag tgttttgctt gtatgtatac 33900 atgtataact tgtgcatgcc tgatgtctgt tcaggtcagg agcaggtgtt tgctggatct 33960 cctggaactg gagatataga tactatgacc tgctatgtgg gtgctgggaa tcaaatccaa 34020 gttctctgca agagcagcag gtgttctttt tttaagttaa tattttagag aatttcatac 34080 acaaatactg ttatttacct cattaccacc ccactcaacc tggatcctac ccccaaccac 34140 tcccttctaa aatttatgac ttcttcattt cttttttgag atttatttat ttattttata 34200 tgcctaggtg cttctatcta tgcacccctt acacggctga tgctcaaaga ggccagcaga 34260 gggcgccagg tcccctggaa ctggagttag agatgacggt ggcttttcct ccctttttac 34320 caggagacct agtcctcaca gaggttttca ggtcagttcc agtttgattc cttcaagtcc 34380 tgtgtccaaa gtgtgtgttg tctttagcca tacgcgttta atgtcaagtt ctgggaggca 34440 ccaaagagaa cagcagaagc cgctgggact tctctgacca acagcacgga ggtttttcat 34500 acacggtact agatagtttt tggctcttgc agggagcatt atagcccaag ctggtgtaac 34560 accatttaaa gcacacgtaa gcagtatatg catatactta cacttgtatt catttatgta 34620 ttatgcatgt gttcttttta gagatctatt ataggtgtat gaatgttttg cccacatccg 34680 tgtacatgca ggacatgtgt atctgatgcc tgagagagtt agaggagagg gcatcaggtc 34740 ccctggaact ggatttacag acagttgtga gcagccacga gagtgatgga gactgagcct 34800 gtgtcctcta caagagcagt tactgctttt aaccactcag cctcgccccg ctacgcgtgg 34860 atttttaagt aaacacgaaa taaacttttc ctttcgcctt ttcaaactca ctttgtctta 34920 tttatgcctc ttccctcttt tccctctatt ttattcttcc ccctcccatt aaagccccac 34980 ccccttttct attcccccct ccctttttgc taggacacag caagtgctct taactgagga 35040 gcagtctctc tagaaccccc gcccacgcca cacatctttt agagctgaaa atttcctaca 35100 gcttccgaaa ggaagaatca ggatagagtt tagaaatata accaacagag gagaagatgg 35160 caaaactact gctgccttca gcacagagag gctttaccta gaaaatgggg ggggaaatgg 35220 aaacatggaa aaatgcattg attggaaata taccgaggac atgggaagtt ttaagggtga 35280 tgagcttgag gcgaggaaac cagatttgta cagttttgga agaaacatgc aaagtttaaa 35340 ttcggctggg gatcagaggc agttatttta tggcaaagat gagattggtt ttgtccagct 35400 tggcctcatt tgtacctctc cattttagct atagtcacat cttattaata cttacctcac 35460 tctttggagc ttaggcagaa aatccattat ttatggttga gtattcacca tttatgtgta 35520 agtaaggaca tctgtatgcc attgaatatg tggttgtgaa gtaattaaaa cagtttacag 35580 tgtaaaatga aagacatttc tctattttaa acatgcaata gagaaatggc attttatata 35640 ttgtttagaa agatttattt atttatttaa tgtatgtggg tacacactgt agctgtacag 35700 atggttgtga gccttcatct ggttgttggg aattgaattt aggacctctg ctcgctccag 35760 ccaaccccat ttgctctggt tggccccact tgctccatcc caaagattta tctattatta 35820 taaataagta cactgtagct gtcttcagac acaccagaag agggcgtcag atctcattat 35880 ggatggttgt gagccaccat gtggttgctg ggatttgaac ttaggacctt cggatgagca 35940 gtcagtgctc ttacctgctg agtgctgagt catctcacca gcattttata ttctaactag 36000 attcaataga cacttttgtt ttgttttgtt ttgtttgttt tttgtttgtg atgaagaata 36060 ctaaggggtt tttacttagt tagcagtaag caagatgaac tgaatatatt gtatcaagag 36120 gcatcgagat tgcatggctg taactcaggt ggtgatgaag gaggataacc atcccctgga 36180 ggctaacctg agctacacag caagacccaa cccaaaagca gccagccaac caaaccaaac 36240 tcaaacccaa aggcccgtga aaaattgaat tctgatgatt agtgccccat aaggacctta 36300 tggaacatga aaacaatagg aagaacaaag agaacgagca ggtcagaacc acacttaccc 36360 aaattaaagt ctcccagctt ggaagatcaa atcatacatt acctcaagta actcatattt 36420 gtctgcaagg tgtcagctga ccccttttct ttaatatctc acttcccacc cccaccccct 36480 tttaaacaga aagacctttg attgtggtgg ccaataattt gaagttttga agaattaatt 36540 ggccctatta tacatgaaga catagtaatc ccccttcact tttggaagcc tcggagagag 36600 tttgagaatt tcaagttgaa atatcacccc ctttcacaaa attctgtatt taaggatagt 36660 atttttcaga atttaaaaaa attttttact ggaaacttaa tattacagca ttccccttca 36720 gggtttatgg tcaagctttt ttaaatatat gttttatttc tttaactggc agatgtttga 36780 aagatgagag taggattaat cctcatatta atttttaaat acatctcatt cttccaaaga 36840 atgccgagaa aaagaaccaa ctctccatgt agtaactggc aaggaaggaa atagggctcc 36900 taccatggca actccctgtc agggatgctt agtgttgttt ctgtccaggt tcctcactgc 36960 tgagaacaaa tagccaacaa tactgagaga cggggaggtt tcttctggct aacagtttca 37020 gggatacagt tattgcagga gaggagagga gaggagagga gaggagagga gaggagagga 37080 gaggagagga gaggagagga gaggagagga gaggagagga ggcagctggt taaaccgggt 37140 ctgcagtcag gaagcagggg agccacacat aaatgccgag actacacttg ctttttcctt 37200 ttgctccact gtgagaacac agcagacctc aaaacacagc agtttgggaa ccaacacaca 37260 cgtctctttg tgtctggcaa ttgatgtaag ctccatggtt tttgcttttt ttctagctcc 37320 tttaattgat tgattgattg attgattgat tgattgattt ctgagactgg attttgctct 37380 gtaagccagg cttgtgtaga actccaattt tggctggcct ggaattggag aagttcatat 37440 tctcattata cagagtgctt aatggcaccg ttatgctcag gtcattcatt cattcattca 37500 ttcattcatg catgcatgca tgcatgcatg catgagatag gagttttctg tgtagctcag 37560 aatttccctc caacttcaaa ttctccttcc tcagctttcc tggcaatgaa aatacaggcc 37620 tgcgccatta tgcgcaagtt atattggctg ttgttaatat ttatgttagg aaaagaaaat 37680 agtctctgtt ttacatacaa tctacttgga atcatttttt ttttttaaag ttaagtcggg 37740 gtgaggggtg tgtgcaggtt aacaagcaaa cgcaattcct ccagctaacg ccaaggggca 37800 ccctctcctt cccctcggtt tctggagcgc cttgagttct ttccatctgc acaaagagcc 37860 cggcctcctc ccgggtgaca tcacaaaggg tcgttctccg ccccttcggc gccaccacgt 37920 gtgtccccga gctgagtggc catccgactc agtccctcgc cggccggtct aggcagcgga 37980 ggaggcgagt gcttgatagt ggctggaagc ttcgctatgg gaagtcgctc tttagcactg 38040 tcgcggctag ccctgctctc tggttctccg cagccgctgt cgttggagag caccgggagg 38100 cgcgggttgc gagcgcgcct gcttctcacc gcccgggcgc cagcgcccct gggcaggtgc 38160 tgagcgcctt tcggagcctc ccctgctgcc tccctcttcc gcctgggtgc ctggctgctg 38220 cagtgcacat gggctgctgg aggtagatgg gctcaccgcc cgtgaggcgg cggtggatgc 38280 ggcgctgggc agaaacagcc accaattcca gctgccgtgg ggccgagcgc cccgggcgcc 38340 gctgcgagcc ccgagctcgg ccatggggac ccgggcaagc agcatcaccg ccctcgcctc 38400 ttgcagccgc accgccggcc aagtcggagc cacgatggtc gccggctctc ttctcctggt 38460 gagcgatagg aggggaccct agggtggcgg ctacgttcga gcctgtgtcc gggagccggg 38520 cgggtcctcg ggcccgtagt gccggaggcg cgcggtggga ccagacgggt tggaagggtt 38580 ggttacaagg caatagccag accgaggagg actcctacta gatcgtgtgt gtgtgtgtgt 38640 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgg tgagctaagg ctagtgagtg ttaaagaatt 38700 atttttcttg caatctggct gcttgaagga ggcgcgagag gtgtccgcct cgaggtcctg 38760 cgcatttttg ggtttttgcc cccgactcgg cctatctcgc accccaacac ccagcctcac 38820 cccaatatca ttcgctgcca gaccctcggc gcggtgcgag gcccgagtag tcctgtcagt 38880 agccagctgc cggttgtggc cgctcctttg tcgctactgc aatgtcatgt ccggatgcta 38940 ccgcagtgga gacagccgga tcggtgcgct ccggacgcga gcaaccgctg catgcttccg 39000 cgcgcgaagc tttccggagt agggcgcagg ccaccccttg cttcagcttc cttgcttctc 39060 tcctccctct tagttcagat gggtcctgat ttctgcaagc caaaagaaga agaagaagaa 39120 ggagcccggg tgggtggtgg gggtggggtg ggagggggag gaggagaaga aggacccaga 39180 gacccaggcg cctctaaagc acaggcttgg agccaaccgg cgctgtggct ctcagtcgag 39240 ggggagggga aagagctaca taaaggacga ttaattttcc ccatcctttt cttatttctc 39300 cctttctgtg tttagacatg tcaccaagca agatgatgca ggctctctca gcacccaaat 39360 ctggggcgtg cccggattgc aatcctccat cactttatct gatcaaaacc tgacatcatc 39420 ccttatccaa aacttacccc cagctggccc tttatttcca atattggggc caagtgggga 39480 attaggtgga agaaaatttg aattcaaacc cttgagatgc tataaaccaa tcatgtcttt 39540 catccctgca gggaggatga aggttgttag ctaaaaacta aacaaaaaca aaaaagagct 39600 tgttttcatg gttgggctgt gtaagggaag gcaggcctct tgtctccttc ctctaaaggg 39660 cttactgcct ggccagccaa gatctactgc ttccatccca aggcatgaca gttgctatgg 39720 cctgggaatt cattctacag acattctggt tctctctgtc tctctctgtc tctctctctg 39780 gctgactctc tttctgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg 39840 tgtgtgtgtg tgtgtttagg gagagagaga gagagagaga gagagagaga gagagagaga 39900 gagagagaga gggctaaaga aagtttgttg ttcttagcat ttatgaggta aatctcagtg 39960 tcccttgaga gacgcttttc tgtcttttta taacaatggc aaagtccaag ccctttaaac 40020 aagtgtggat agtaaataat cagagagtct ttgggaccgt ggacttgaat tgacagtctt 40080 agattgaacg agttcaagga agcactgatc ctaatgacct gagttaggtc ccttgctgag 40140 ttccctccaa aggtcttaga cctgcttccg tgatgagcat atactgccgt gtgtccctgc 40200 tccacacaga tacaatcctt atacaatact tatagatcag tccactcaaa tccaatctaa 40260 agttagtgct caatgtatag tcgtaatctg gaattatttt ttaactttta gcctgatata 40320 taggtccttg aaggtctcag catagtaaaa ccatatgctt taagtaacca agaaagggcc 40380 tagttctttt tattaaatga tcaaactcaa tgtacccaca gattgcaccc agatttgcaa 40440 ctgatgacct acagtataag tttgacagtt tgatgaagtt tatgaaaggt attttagaga 40500 caatctcaag gtgacgatgc tacagggttc cttgatttcc tggtaactat ggcaaaaggg 40560 ctttctttaa attgatggag taggaggagg gtgagctgac catcctggag tgtgggaata 40620 ctccaggaaa catcaggagc tatgcaatga ccttctccat ccccactttg gacggtccag 40680 tcatcaggac gggccctgtt tctgtataca acagtgatct ggtgactctg ttctcaggag 40740 aattggtccc attggtaatc ttggccacat taacagcaga caccaggtag gacagctcaa 40800 gtaaacatgg tgtgaggtac ctagaagaat aatgggggat acaggaaggt taatgctatg 40860 tatatacagg gtggttagat aaagaaataa tgatggcttc caattgccct tctctcattt 40920 ttttttcata gcagtctttg ttgttttttg tggctctttt ttttttctat cagcatgaag 40980 agcttactat aggctctgat atgtatgcct atcatatgaa cttgtcaaaa gagcagcgag 41040 ctaatttata cagaatatgc cactatccct gtcaaacggg tgtcacctcc attctacgga 41100 tgagaaaata gtctccaaga atcttactgg tctgttgtag tatgtggtga ctctgggctg 41160 gaactccttg gctcctactc tctctcaccc acgtaccaca ctgcccgttt cacaacttca 41220 agttgtttgg tgagtgtaag gacagagaag gactgctggc aggtagctga gacggtggct 41280 tgattaattg gggaatataa gtgagacgtt cacaaggacc tttggagtga gtcagtctag 41340 ttgctggcat ttctattgct gatgtgttga tgtgaccgtg tcatgtttgg aagttactca 41400 caaactgaaa tgcagcatac acgttccagg ccaaagttct gtaaatgaga catttagatc 41460 tgtagtggtt tagggattgt cctagaagga cttgcttact gcaatgctgg atgcagtcag 41520 aggtgcctct tgcctttagg gagcttggtt tgactataca gatagactga ttgcaaggga 41580 tggtcaaggg aggaaagcaa cttccttggt aaagactttg gatttggagc tttggaaact 41640 gctgttatcc acggactgag aattcctgca ctgaaggcac agggaagaag gcatagcaga 41700 agaaggagtg gaaagattct aaaagccaga ggaacagaag ttgctgtgag atcgtgtcac 41760 ctggaaatgt ccgggaagct tcactcatga agtttcaaca gcatggctgc gtaagcggga 41820 tctgatcaga gaacaccagt ggacaggcta acacagaagg gggaacttca tagggcctca 41880 gccctagaca aaaaattaga aatatgggct actaaggaat atatatgtgt gtgtacaata 41940 cgtgcatata tatacaaaca tatgcacaca cacatatatg tatgtgaatg taacacatgt 42000 gcatatgtat tatgcacatg tgcacatatg taatagcaat tgaaaataac gccatgaatt 42060 tgagtcgtgc aaggctggta catgagaggt tggctgtcga gaggaaaggg aaggaagaaa 42120 agatttccac acaagaggtt gtccagctgt ctggccatcc cttttctagg cagctggcat 42180 ctcaccattt ctggtcacca aaagaaaaaa taccttattt atttatttat ttatttttct 42240 tttgccggtg ggaaatggtc agacacatca tcagacacat catcagacac atcatcttgc 42300 ttagagggtc ccaagtgtta ctgttggcca ctgctcctga gttctacctt ctgacttcca 42360 actatcacag aaaatggcta tctgcctggg caggagtgta catgtgaggt catctggggt 42420 ggggtggggc actcaagtcc tagccttggt gacaaatcca attagaatat gaaatcagag 42480 ctggcgggca tacagagggc tctctccctt tgatatactt taacacagca tcctagactg 42540 tggaagctgg gagggaagct aagctgttac tgatctaaca ccccacacag cttggctgag 42600 ggaaagaaca gcctccactg cggaagtgac tatttttcat tttaccaaat ctacatggtg 42660 cagaggcggg atcctaagca gtggaagccc agtgatgaag tgggattaga accttgcgtg 42720 gcatgaagct catttctgtg gcttataaaa caagtggccg gtgtcaatgg atcaatgggt 42780 gagcatgcta gaccccgaga gagacacaga tgtgatgttc acatcttcaa aatatgtagt 42840 ctgtcagagg aggaaaacac ttctattttg gaaaactttc caatcttgga atgatatatg 42900 ctagttgatt tatgggaaat tgttaatgag gaggaacaag taatccagat cttctactgg 42960 attgaacaac tcctgatttt taacccataa gttatcccca caaagtttag agtagggtca 43020 agctttctca tttggactta ctgtatatct ctggctatca gatttagtca cagacagaca 43080 gacagataga cagatagaca gagtctccag gacaactaag caattaagat gaatctacaa 43140 gaaaaacctc tgtagttaca cgggcacagg tccactgact acttatacat ctggacaatg 43200 tctaccaatt agataaagca accatagttt gccttcccca gtggtcaggg gtgggtttgg 43260 cttcagagct gggtctagtg agtagctggt gtcttctctt tggaatttgg ggacatcaca 43320 tcttgacttt tgcacttgac tccatcttga ggagaggcaa agcttcttgg gaggcttggt 43380 agaaaagctg caaattggct ctgtgctaac tcagccagca ggcaaactat aattggctta 43440 cataaagttc acgattatta ttattatttt tttttctttt ggtgacggga aacggtgaga 43500 tgcatcatca gactattcaa gggccaggac gaattgagcg acttttcctt gcgtaaacag 43560 ctctgcctat tttccagaaa gcactaatac tctgttggct tgcttccccc ctgcacaggc 43620 ctggcaaatt gcggtaatgc caggtcacca ccagcaactt cttggcaatc tcaggtgctt 43680 ctcttctgtg actagaaggg cccagattaa ctggtgggta tccggtggtc atttggagtt 43740 gagtccattt ggagcacgag atgcagatat attggatgga cagaatgaca actgaagcca 43800 ttagtaggag cagcttggcc cactgagtgt cagcaggagg ctggacttgg ttgtttctgc 43860 attagaggcc actgagttgt gttctttagg aatagaggcg agagatggtg gataaacact 43920 ggctttctcc agtgtttata tttgtcaaat acctaaaaat caacaggatc ttaaattggc 43980 ttgaaggctg ccacctggtt ggacagtgat tattaacctg gagggagact ggcagatgat 44040 cagaaatatg ggtggggcct ctgcttatgg agagaggttt tgatgaccgt gctgttgaca 44100 gaaggcagat gcgcaatcaa cattcatgaa gagtgctgaa gtcggggtcg tgtgtgtcct 44160 ggttggaggc aacatgtcta tagtgttagg aaagttgtgc tcagggggct ctcaggaagg 44220 tgtgggatgc agcaggacct cggtaagctg gcaggatgct gccccatgtc ctttgatctt 44280 ctcttcagaa tggttgcaga ctatgcatga ctcgtcgcag gtctaagaca aataaaaaga 44340 attggagaga agcacatagc caaatcagaa cacggctagt agagctatga gacaggccta 44400 tatcgtgttc cattcgctct ctgcaaccag gatcactgta gtggctctgt tgcttggcac 44460 catttgcaaa atctgcttgg agggaagagg aacacaggct taatagagtt agacaagaac 44520 atggagcttg tccatgtgcc ctggcctcaa gagagcagct gtgaaatcag cagagctcct 44580 gtgttagtgg cctttggagg aggactaagc aaatggctgc taacccttta tattgggatg 44640 ttatttaatt tatatattat aaaaatatta tgtaatatat tcattcatat actatagaaa 44700 tggttcttat tactgcattg aatatcttct gtaaagcact acagtgtaca ttttacaagt 44760 cccagacatg acagctttag tcccatttta cagaatccag aataaggttt gatagagttt 44820 ttgctcagat atctgcccat tattacgttc tggagagagg gatgaaatct agctcagcat 44880 agtttcaaac acttagctct ctctgccaag agagaatgga acaagtgaac agaatggttc 44940 acttcaactt cccatacata cgtttgttta ttctaaaggg aatggtttta cccaagaggt 45000 gaacatgccc gttttattct ctgagtttca cagtggtacc tgaatggttt gccaaaacta 45060 ctggtacttg tgagcatggt gtgtgggagc tgaaagggga gggcgtggag acattgggcg 45120 aacactttcc tatggtgtga ctctgacgag ccatggtgcc acggttgaga tggaaagaca 45180 ccccagcaaa ttgaaacttt ataaataggg aacgggcgtg ttggctctcc tgcagccctg 45240 ggtcactttg aaaaatattt gcttaatgag taacagcgag gtgtgggctc cactctgctg 45300 tgtagttagt ttctgctcct ttagtctgag cacctgagga cctgctaggg tctttgctgc 45360 tagcctgact ctttgtatag gataatagct gtaccagcaa tgactagcat ctgtccacat 45420 ttcattccgg ttctcattcc atggtttgag tggttcgtgc taggaatgta gtggataggt 45480 tgccacagtt actggagcac cctcataaac aagccgttag agaaaaaagg agagagcttc 45540 atacatgcat aggatacgtt ttgatcaaat ccacagccct tcactctctc tctccccttt 45600 caacttctcc caacagcccc ctctccccat cacctttccc tcccaatttc acgtgctctt 45660 tctctaaatc cactaagtcc gtgcacctgt aggtacgtga gtgtaggact atctactgcc 45720 gcatggatag cctgtcagga gccccatccc caaagaaaat ggagtcttcc tccccgaaca 45780 gccatcaatt cctaacggct tctcagctag gggtaagaat ttatgactcc atctcctgcc 45840 cacgcaggct tttgactggc ttggttttat acaggtcttg tgcatgcagt cccaaccatg 45900 cacacttcat gtgtgcaggg ccctggcatt tctagcaaat actgtttcac tctaatcaaa 45960 tgatcctttt ggatcttaca agttttctcc tcttctttga gatccctgag ccttgtgggg 46020 gaggggctga gatgcatcgg tctcattttg cacctaacac tccaaggtat tctaggtatt 46080 ctattctctg tccgttgacc aggcgtaggt ctctctctct ctcttttttt ttttttttga 46140 ttcccatctt ctacactgtt ggaaggggag ggagctatgc tgattaaatg agctttgaga 46200 tctgttgaaa tcccaatttg tagccttagc agacaaaagc tggaaggaaa tgatgtatta 46260 gtgccagaaa agagattgga gttctggtct cccaaaaacc tttatgacaa gaccactgtc 46320 tgatttcact gcagacatgg ctagctagct ctgctcagca aggactcaga gaggcagcgg 46380 ggtacgaacg agacaaggaa acttgcaggc aaagatgaca ggttttcaga acagtccaac 46440 ttgtatgttt gtgcttattt tggccccact tttctttcca taattatttc cctttctcct 46500 acaggggtat atttgtgttt ttcatcaact tactaaagcg taaagactat ctactaagga 46560 acaggcatgg ctagggtgtg ggtctataga agtgactaca ggttatctgt agtctcccct 46620 tggagttcgc agaaaacgtg atgtttcatg gtaatcatca taaagcagaa gggagtggat 46680 ttcagtggaa gggaccggta tgtagaatag ccccagtgta ggacagagtc tgtgtcttca 46740 tctctgacca ctatgactgg gaagaggcag tgggactgtg tgtcgtggga cccttgaaac 46800 tatgaagatt cgttcagtgc aggagaagca gggtaataac atggcttgaa acattctttt 46860 tttttaaacc ttatgtatct ccagatctca catctccaga aagttcctgg cagtcttgag 46920 acactaatat gggttctggg aaccaaatgc aggcctctgg aagaacagca agtgcttaac 46980 cactgagcat cactcccacc tgccaacatt gaaaaaagaa gttttaatta atccttagag 47040 aattccattt agtgtatttt gattccattg ctcctgaagc tcctcccaga tcctccccac 47100 cgcttaactt acccaagttt ctctccctct cgcgcctcct tgcattgagt atagtttgtg 47160 ttggctaact gcttttgggt atcaggccac aatcaataag gaaaaaacaa aacaaaacaa 47220 acaaacaaac ctgtttctca tactgaggat ggaacctagg gttttcacat attgagcaag 47280 tgctctacca ctgagctgta ctcagctctc agatcgctcc agaagcctgc tggaggatgg 47340 aagctggagg tcagctgctt tcacagggtg gagatcacag tgccggggat agtggcggtg 47400 aagatgaatt ttctagaatt tactggtggt aagatgggag ggggtcaggt ggtctcaaag 47460 gaggcggact cctaggtgcc taaattgaag tctgtgattg gaaggatggg ctgggggcgt 47520 ggcgggtaga attagaaccc taagcttctg ctgtgtagtg agttaagttt gaagtgtcgg 47580 cgatgaacat tggcttgggc cttgattttg ccattttgaa atagctgact ctctgcaggt 47640 ccgaagcgca ctgagccatc gcgactgtcg ccaggcattt gggctgccgt ccttcctccg 47700 cagcatccag cagcacagac atctgtgtgt agagcaatgc tctgttcccc cttaaggttg 47760 cttcctctga ttgcattccc caaagtggca ttgccaggtc agaggaaacg aatgagtggt 47820 ttttatggcg ttcgctgcag attgccagat tgttttttag gaagacaatc aattcagcca 47880 ccagccacct ataaatgtgc tttctagtcc atgaatctgc cgcgacggga ctttctcatt 47940 tcaaattacg tatcttcttt ttaattcagc gatgtaagga cctttggtgg tctctgtgac 48000 agtttagtca tttctagggg cataataaac ttacttgcat gatgcttgac ttttaatgac 48060 tcatggtcct tctagcgagt agaagttatg ctaatcttat ttttatcaat tctacttatc 48120 attctggtta taggttctta ctagctgtaa tggcattgct ttatattcta ttgacttttt 48180 acgatcatgt ttacgtattt gctttgtttg tgtgcacgtg ggcatctctg tgtcacagtg 48240 catggaggtc agagacaatt tatggagtct gtgggtcctg gggatagaat tctctacctg 48300 tcaggcttag tgttaagccc cattgcccac tgatccattg caccaatact gcaccactac 48360 tgtactctac tataatatca tgaaaaaaat aggattgtgt tattaaacct tttcctttct 48420 gggtctgaga atattctttt tgttttgttt tttggatttt tttttttttt tttttttttt 48480 tttttttgcc cattttgtta ggaattcatc ctttgacagg tagttataaa gaatcctaat 48540 tgctttgaat tttataatgt tattggttga cttgttgata tttctggata ttggtgtcgt 48600 atatgtggtg ggaatcccct tggtgtgttc tgaaggctat atgtgcgtgt gtgtgtgtgt 48660 gtgtgtgtgt gtgtgtgtgt gtgtgtaatg gattctgtgg gtaaacacac aggggagtgc 48720 tgggcccagc tgattcacca tttcaggctg ttcttccgtg gagtcacctg ggacaggtga 48780 tagctgatat attaattgta cccgttcagg agaagatata cggagtattt tctgaactct 48840 agactagggc atcagagact ttttctgtaa agtgctgggc agtacggtta tcttatgata 48900 tctgtggtaa ctacttaatt ccacctttgt gcaagacatg tgcagacaat gtctcgaaga 48960 gtgagtctgg ctgtgtttca ataacacttt atttgtggat aatgaaattt gagtttcaca 49020 cagttttcac tggtgctata ttgctactca ttcattcctc ctcatttccc agctgtcaga 49080 ctataaaact gttgtaggcc tgcctatagg ctatacaaaa atagacggcg atagagtcca 49140 gcctgctttt agaccagggc attaatattg tgtgagagca aattggggca actcactttg 49200 gaaaaccctt acttaatcgt gttctgggca tctaaagtga gattattaat tgtaaatcct 49260 ttctatttta ttattatttt ttgttataag ccaaggttta tttcatttta attaattaac 49320 ttactttgag gtagtgtctc tggttgacta ggctgaccca aggctaacct tgaattgcct 49380 ccgttttaag tgctgggatt atggtgtgtg ctggtctcgg aactcaccag gcaggcgctc 49440 aaatagctga gccagccaca tccccagaat cccaagtgtt tgcttgtttg tttttaatat 49500 agtgtgaaaa gtagccttgc tttctcttgt ctttatcttg acgtctgctt taaaacaaaa 49560 cttctacagt gtgctctgtg ttcggggaag tgagtttcag gattataatt ttttattttt 49620 taacagtatt attattatta ttggtatttt tgagacagag ttgctctgtg tagccctggc 49680 tgtcctggaa ctcactttgt agaccaggct ggccctaaac tcagaaatcc gcctgcctct 49740 gcctcccaag tgctgggatt aaaggcgtgc gccaccacgc ctgcataatc ttttattaaa 49800 ttaaataaaa gaaataacca atttttggtg tttgaggaag aatatcccta tggaaatcag 49860 cttgaatagg aaatcagtgt ctagcacaag ctggccttgg attttgtgat gttcccgtct 49920 ttccatccta aattctgtgg tcataagtat ggaccaccat gtaaataggc aagtaccact 49980 agtacatagg catgcatcca gatggcagtt cttttcttat ttgcttgttc tgagccagtt 50040 tgggtgtgga tccctgcctc actttgaata cactatgtat atccatctgg ccttgaactc 50100 actaagtaca ccaagctggc tttgaactca ctgcatacct caagctggcc ctgaacttgg 50160 gatattcctc atgcctttgt tcccaaagtg ctgggactac agtggatgcc actgtgatgg 50220 agttgggtta cacctttaaa caaaaaagtc tagagtttgg aggctggaaa tgtaacctgc 50280 ctagcacaca gacaaccttg gacttaattg tagcatgaca aaacaaaaca aaacaaaaca 50340 aacaacaaca aaaacaaaac aaacagcaac aacaaaaaca aaacaaaaca aacagcaaca 50400 aaacaaaaca aaaaaggaaa catttctcaa atttatactt aaaatagaat tgaaatgttt 50460 taaaacattg tctattcaca ggtaataaac ttaaagtcta gtaaaataat aaattggtgg 50520 ggtgataatt tcccaaactg gtattccatc cagcaattac catgtcagac atgttctcca 50580 tactccttct gtgtagctaa ttatcaaaac aacttagaat ggccgttgtt attgctgtgt 50640 ccattttaca agcgaggagg tagaggcttg gacgttggct ccttcccagg ttagaaagat 50700 agaaaaaaaa aatgtaattt tcttcagctt aggaaccaga ttcaccagct gacactcaca 50760 gggtgtcttc aattcacacc cgaccctatt tgcttcaaga tagctaccgg ttggaaggct 50820 agattccaca agacggactc taacggacgt caggtatcaa gagtaaaagt caggttataa 50880 cctgtgcttt ctgttgtctg actgtaagtc agagttccta tggctctcag ggggttcagt 50940 taacttgtaa gcatggcctc acatatctca gggaaacact ttctgaatat ggctctcttc 51000 tacagaaagg agacccatga taaagcatgc agaggaaggc atgcttaggg cagccatata 51060 aggagcaccg tggagctgtg gagctactgt gtctgtctgc cttggacacc gtgagctctc 51120 tgaaccttgt cctttcagag ttttatggag atgctatttc ataggcacaa tttactacgt 51180 cattagacac tggtgactaa acttaacctt caacccctct cctcatccca gagattgagg 51240 tagaactgaa agtagcaagc tgaatataaa aaccttgtgt gttctgacca ctggtctcca 51300 tcttggtggc accctctcag agcctccgtc ccctgtcatt ttatcaccac ccagaagacc 51360 ctaatcacgc tggctgtgag cttccctact ttgtaggatc catgggaaga gaccacatct 51420 atattcctta ttatcacagg atcgcggcca gcagatacct gggcgagcag ggcgtatgtg 51480 gtacattcta ggcttaaatg gtttttcctt atactttgaa gcctcagttg ctgcactggc 51540 ctgggttgta tgctcttcca gacatgccca gtcagcatga agttgcatcc ttccatcatc 51600 tactgttttc tatgacatac tatttcactt attactcact gggcatccta ttggcatctc 51660 tcagtaagca gagaataaag aaattactac tgtgttggag ggagacgggg ctgtggtcca 51720 gtgactgatt gctgtatgca ctttctcttc tgcattgcgg gttccagaga aaacagctcc 51780 taggtgaaat cttccctgtt tttttcctct ccagtgtccc ctgagcttgc ctccttctgt 51840 tcctacagtc agcacattct ggctctggca ttctctatag tcaccagctt gaaaaacagt 51900 ccctgcttcc tttgctttgg caataggaga cggctctccc tccttcccat tcctctcatc 51960 cctcccatcc tcctcttcct tcatctctcc ctctcttcca tccttccttt cttccttcct 52020 ctttccttct tttcagagaa ggcagtagaa atgaaagagc caaccaagtt tatataacat 52080 aactggaaat accctgtctg tgatttgtgg aacctttgtc cttgaaacgt ttgtttgtat 52140 gtatcccacc tgccccacaa cttcagatct ggggaattat agccatccag tgtttatatt 52200 acaaattaaa aatgatggcc cagctatctg gtcaacaatt ctgagggctg agcagctctg 52260 tctttgtcat caggtatcat atttcatagt catcctcata ggcaaacctg attaccttac 52320 aatctctaca gtaactacac acacacacac acacacacac acacacacac acacacacat 52380 atatgtatat acacacacac atacatgtgt gtgtgtgtgt gtgtgtgtat ggaaaaattc 52440 tatatcaaag tccagagcaa ttttcctgct tcttactaag gaaaagcagg tccacctctg 52500 tcttaagttt gctcgtttta taatgaaatg ttactagaac ttgagaggaa atgaaagtaa 52560 tgtctgcctg gtgcagggac gatggctcag tgggtaagat gctggcttac aaatgtgaag 52620 agatttgagt ttgaatcctt agcactcatg taaaaaggct ggatgtggtc ttgtgagcac 52680 ctgtaacccc agcattatgg aggctggaga caaggaggct tgctggagct tgctggctgc 52740 cagtcttgtt aaaatggcaa gctccaggtt tgatgagaga gccctgtctc aagtcaacga 52800 agtggagagc gatggagtag gacactctac atcctcctcc ggcttccccg tgggcatgtg 52860 cacctgtaca cacacatgca tataccacac acacacacac acacacacac acacacacac 52920 acacacacac acacgctaac gtcaatggca cagatctgtt gtaaccttta agctaccaag 52980 ggatctcttg tttatctgaa ggatgaggta atggaagtgg aattgctgaa caataagtag 53040 atgtctacat tctgacatgg attcacacat ccgggtgctt cttgtcttca gtcaggccca 53100 cctgtgctgt aaactgcaag tgacacatca taggtgtgct ataggccacc aggatgcaca 53160 gtgagactct ggaagccttc acagtcagtc acctgcacca agtgctgctc tccacttgat 53220 ctctgcttct ctcgtttaag cttctaatca ttcaataaac acttgtagtg tttttggttg 53280 ttgttgtttt taaccaaata cagtttgaga cagcatgctg atacttgatg catctatcca 53340 ctgttgtagt tgacattcct tttttttttt gattagatgt tttctttatt tagatttcaa 53400 atgctatccc taaagttccc tataccctcc ctccgccctg taggtgacat tcttagatac 53460 atggaatttt cataataatc gcagtttaaa cacagagaga atctaacctt cttatattgc 53520 ttataatctt aagcacaaag tgtaatcttc tctttttttc tttcttttaa atgagagaag 53580 aatatttgaa ctctttagta tattagcttt ttattttgag tatcacgaag aagctaattc 53640 ttagaagcca attatagttc tgttaagaga gagtaatggg gggctggaga gaaggctcag 53700 aggttaagag cacaggctgg ccaggcatgg tggcgcacgc ctttaatccc aggacttggg 53760 aggcagaggc aggtggatct ctgagttcga ggccagcctg gtctacagag tgagttccag 53820 gacagccagg gctacacaga gaaaccctgt tctgcattat agattccagg gaagacacac 53880 agagtataag tgtgtaagta agaaaacgat gacaaccata tagggaaaag aagaaaccga 53940 ggagggcttt acaattgggg atgggaggca gggagagcac ccaggaggaa gtcttgtctc 54000 tctggatgat tttgcttttt ctcagacgtc agggggaagt gagagtttga agccgagtgt 54060 gaaggaacca gattgagcgt gtgagttaga gtgggtggag agagagagag agagagagag 54120 aaagagagag agagagagag agagagagag cgagcactct gctcataaag aaacttatgg 54180 gggagaccag gaagtttcta tgagcagctg gtctctgcaa tgtttgtatt tctttagatg 54240 cccatgctgg gagctggcag atttcaccta taaagggcca agtgataaca gtaagctaac 54300 tgaggtttct gggcagaatg gtctctgtca tagctttgtc gctttcccac agtagcctga 54360 aagcagccac gagtgctagc tacatcagtg tctggactct aggaagcttg tttataagac 54420 gagctggtat gttggcattc ctttgctggc ccctggcttg aaggatggtt tgcttattat 54480 tttcctagtg ttcattaggt agttgagcaa gtgaggaagt atagtctgta cagtagtccc 54540 tggccttgag ctgcttataa gcattaagga aagataagta tatacactta cggtactgcc 54600 aaccaacatt aagagacact atgtaattag acgctggtac aatagacaga aacaggaagt 54660 gcgatgggct agttgctcat ctgcttggca tctgggtgtt tagaaagaga tttaaaggct 54720 acataaggat ggcaggattt gacagaatca caacacgcag gagggtagaa ttggttgaat 54780 attactactg agttcaacct gctcatttcc taagtgagca acccaggatc ctgttgactt 54840 cccaacaggt tgggtaagaa catgccttct cttttcaaaa tctttgtcca gttcttttcc 54900 tgactttttc tgcttgatcc acccagggat gtttatgtac gtatgtttgt gtatatgttt 54960 tatattgtta ggggtgagag aatggggtcg ctccaggtta tcgattgcat ttaggatgtc 55020 aaaagactgg gaccagggtg atcgtgtctg ctacttggac tgatgtggag agaagagaag 55080 ctaggtgagg ggtatgggag ggaagaagca gttgtgcaca atagaaaact ataccctgac 55140 ctatatacat gcaagaaagc agtcaatgaa gtcttggtgg gaagaccctc tgaagtaaga 55200 catacccatc ctgttggttg cgtgcctagc ctttaatggc taagccagct ctgtagaata 55260 tgctcattct gttgaagaac tctgaattgt tagagtgaga cagtttgtat gttaaaacag 55320 acaggtcttg gtgatgtaac agaagttagt aaaagagaaa tcacagagca acatggttgg 55380 ctttttttcc ctttgcatac acaaaaccaa ctcaaacaag agaacgggga agctgagaat 55440 ctgtagttcc aaattgctag gactattaca tagatgttta ttgagggggg gtggaagtaa 55500 aatgctaata tcaagttatt taaaaatgcc aggttaggta gagaaggggg tgactctgac 55560 tgtgagattg ggttgtatcc aaaatgtggc tcaggatcaa agaattttac agtcagaggg 55620 gtcttggttt agtgttaggg actcaagagt tagttagata ccagaaacac tcagattgat 55680 taaaaaggca agaaaaaaaa aacctcacag ccaaacaaaa taagttcctc accctgttgt 55740 atactcacaa cgatctcaga gataagacga ggtacatgaa gaaagtactt tcaaaaatca 55800 tctcagacag tattattttt tctttttcca ttttcatgtg tgtgtgtgtg tgtttgcgtg 55860 tgtctgggct ctcatgtgtg gttccatgtg cacatggagc cccaaggttg acattgggag 55920 ccattctcaa ttgctcttcc ttcctatttg gcgaggcagg tgcttaagcc aaacccagag 55980 ctcagtgata tgtgtcatct ctctagcctg tttcttctag ggatccgctg acacctctgt 56040 gatggaatta caggtagact gatgtatcca cttgatacac actcttcccg ggcaatttca 56100 gttttatact caaaagccgg aaactatgtt agatctctct gacctgtctt agccatgtct 56160 atacacatgg tgggggttgg gagaggaatc aagggtctta attggaagga cccagcctcg 56220 gttttcttga ctctcactat ccatgacctt gtctcccctt tcccatttgt ttgtcttctc 56280 ctctagggtt agctttcact ataatctttt tatattgtgc aggggccatc tttaaatatt 56340 gtcttccatt tggtctattt ctgcccagat ccatggggtt agagggaaga gctaatgtgt 56400 gaggtgcaca tatgtgcatg tgtggatgtg cgtgcgtgcg tgcgtatatg tgtgtgaact 56460 atgtatgtat gtatgtatgt atgtagaggc atgttctcag gtagctcaag tgactctcaa 56520 acttgacgtg tagccaaaga tagccttgaa ctcctgatcc tcctacctca gcttctcaag 56580 tgttgggttt acaaacttgc gccatcacaa ggggatattg ttttgttttg ggacaaagtt 56640 tctttatgta gctcagactg gtctggatcc tgtgatcctc ttgtctgagt ctccctagta 56700 ccggcaagta cttccacaac ttggaagggg aaactcttgt ttagatacag tttttccttc 56760 ttcttcttta aatagcacag tgggattagc tattgactct ggaattggga gagactttag 56820 aggaccctgt agtcttgttt gagtaataag ctctgctctc aacaggcctt ccctctgatt 56880 aggctttgca aagcagagct gctctgtttg agacagaagt gagggggctt ggagcctacc 56940 tcccattgct ccttggcaag aggcagccct gtgatttttg tccagttcag cgcttgcttc 57000 ttgtcccact ggtttcttcc tccatccact tcgctaaaca cagttaattc acactctcct 57060 ttattctgtt tgcaggagat agatgaggta attttatagg tcagcattat agtcccttaa 57120 aaactgataa aggagccagg catggttgtg caaactagcc cagctccgtg gctcatacct 57180 gtaatcccag cagcaactgg agagtctgag tcaggatttc cacgaggcca gcctgggtta 57240 taaaataaga ctctatctca aaacaaaata tcttcaaaat aattttaccc ccacgaaatc 57300 aagaatccca aatggagcaa gcatggtata gagtgtaggg aatgtgcagt tgctgggtgg 57360 ggtttgaaca cagctaagtg accccaacaa gtcagacatc aggaaagaac ctaccatccc 57420 tgtcttagaa caaaagtctg gacccttgtc tagtctggtg ggtgttgtca gcaatgtgca 57480 cactcagggt ggctccctgg atcaggagcc ctgggtgggg aaggctgggg ttgaggctcc 57540 atttcctaga gctttgtgta tctccccagg gtgcacactt ttgagtctac agtgggggtg 57600 gggcactaat cttgtactcg ggctccattg gacagatggt ttcctccacc ctgggcatct 57660 gctgaactcc ggatagggtg tctgggctgg ttgtgcaagt agagggagca ggaaaaggaa 57720 gggccaagcc aaacgctgag agaggagact taccaccaag tagcaggatc ttctctttct 57780 caatttccaa tatgaaatta aatttcccaa caagaaaacc aaccactatc catcgccaac 57840 cacctctgcc cctactttca gactcagaag gaagaaaact aagtatatcg taaactctaa 57900 ggaggaaacc tcaagaaccg gtgagaggcg gggaagtacc ctggccccac ataactgtgg 57960 ggttgggaaa agaatagact ggggtcaagt ttcgagatgc taagaagaca gactcttgat 58020 caccttgcaa gcagtttttt ttctttttct tttgctttgt tctttcagtt accgatgtag 58080 ctgtaaatct gtaaatctag aaggaatttg ttcggtagct ggtagcttgg ggcagccatg 58140 attttcatac aaatttctcc atctcactga ggatggcaaa gactatcagg gccttcattt 58200 tgacagcccc agggaagcag tcactctagt cttttgctcc ctgcttctct ttcacagcat 58260 ctcaggactt ggaacttggg atacccccag gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 58320 gtgtgtgtgt gtgtgtgtgt gtggtttgtc cagacagggt gtctccatgt agccctggct 58380 atcttagaac tcaattggta gaccagacta gctccaaact cagagatccg cctgcttctg 58440 cctcccaagt gctgggatta aaggcatatg ctaccacacc caacgggtcc tccaagtttt 58500 taatctggaa gttggtgaaa ctctgacctg ctgggtgaag tttttatttt agaacatgaa 58560 cactgagtta ttcttgctgg acccaagctc aatgggtaga tttctcaatg gatgcctgca 58620 gggattgtgt ctatcggttg tggccttcag agactggaca aactgcgttt gaatctaaaa 58680 gcaccagctc cattgtttaa tgcacataac aggactgtca aaagtatggc tgtttgttaa 58740 aacaaaacaa aacaaaacaa aacaaaacaa aagtttatca tcaggtatgc aggtgctata 58800 gaaatgacaa ctttggcaca cacctccctc tttctgatgt caagttggtg tggtacccgc 58860 cagatcttga acagtggtga tgacctgtta ttgctgttgc tgttgctgtt gctctgttcg 58920 cctctgctcc tctatctgac acctctctta tttctttcag cttggattcc tcagcaccat 58980 cacagcttgg tacattctgg tgccaagtgt ggccattccc ctggctatcg ccttcctctt 59040 cttcttcatc tgtgtgtgcc gcaataacca gaagtcttca tcaggtaccg atttaaatga 59100 tccagtggtc ctgcagagga gagattggga gaatcccggt gtgacacagc tgaacagact 59160 agccgcccac cctccctttg cttcttggag aaacagtgag gaagctagga cagacagacc 59220 aagccagcaa ctcagatctt tgaacgggga gtggagattt gcctggtttc cggcaccaga 59280 agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc 59340 ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtga cctatcccat 59400 tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt 59460 taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa 59520 ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagtcgttt 59580 gccgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat 59640 ggtgctgcgc tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg 59700 cattttccgt gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt 59760 tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg 59820 cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt 59880 cgccagcggc accgcgcctt tcggcggtga aattatcgat gagcgtggtg gttatgccga 59940 tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa 60000 tctctatcgt gcggtggttg aactgcacac cgccgacggc acgctgattg aagcagaagc 60060 ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa 60120 gccgttgctg attcgaggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat 60180 ggatgagcag acgatggtgc aggatatcct gctgatgaag cagaacaact ttaacgccgt 60240 gcgctgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc gctacggcct 60300 gtatgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga atcgtctgac 60360 cgatgatccg cgctggctac cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga 60420 tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc acggcgctaa 60480 tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga 60540 aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga 60600 tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc 60660 tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg 60720 cggtttcgct aaatactggc aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt 60780 ctgggactgg gtggatcagt cgctgattaa atatgatgaa aacggcaacc cgtggtcggc 60840 ttacggcggt gattttggcg atacgccgaa cgatcgccag ttctgtatga acggtctggt 60900 ctttgccgac cgcacgccgc atccagcgct gacggaagca aaacaccagc agcagttttt 60960 ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag 61020 cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg caagcggtga 61080 agtgcctctg gatgtcgctc cacaaggtaa acagttgatt gaactgcctg aactaccgca 61140 gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc 61200 atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag 61260 tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg aaatggattt 61320 ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct ttctttcaca 61380 gatgtggatt ggcgataaaa aacaactgct gacgccgctg cgcgatcagt tcacccgtgc 61440 accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt 61500 cgaacgctgg aaggcggcgg gccattacca ggccgaagca gcgttgttgc agtgcacggc 61560 agatacactt gctgatgcgg tgctgattac gaccgctcac gcgtggcagc atcaggggaa 61620 aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac 61680 cgttgatgtt gaagtggcga gcgatacacc gcatccggcg cggattggcc tgaactgcca 61740 gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag aaaactatcc 61800 cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag acatgtatac 61860 cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg 61920 cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc aacagcaact 61980 gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc tgaatatcga 62040 cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat cggcggaatt 62100 ccagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat aataataacc 62160 gggcaggggg gatctaagct ctagataagt aatgatcata atcagccata tcacatctgt 62220 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat 62280 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa 62340 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 62400 caaactcatc aatgtatctt atcatgtctg gatcccccgg ctagagttta aacactagaa 62460 ctagtggatc ccccgggctc gataactata acggtcctaa ggtagcgaca caggggcact 62520 gcattctggg aatcaaccta ctggcggggt gatttcattt cgtttctgac ttttgtgttt 62580 tggtgtgtat gtatgtgttt aacagagtgt atggccggtg agtttgggtt ctttctttct 62640 ttctttcttt ctttctttct ttctttcttt ctttctttct ttctttcttt ctttcttcct 62700 tcctgaaagt gaatgtataa agcctttaca atgtataact gttggaaaat gcccaccact 62760 aaattttttt ttaagttcca catattctcc atttttgcct tcttatatat atcttcaaca 62820 ctattctgtg cactttaaaa acttaacata aacgcagtgt gacttctccc atatgctggg 62880 tcccgagact ctcaacttct taaaaaccta atggcatctt gtgactccta gaagtagaca 62940 taagtctttc aaccttcaca cctactcttt ctgttttaat tattattgct atttgtctta 63000 ttgtttgtgc tttacaagcg ttcttgagga cggagggaat ctacgaccct gttgatgact 63060 gtaactctat tcgactttga gttgtcttct tcatgtcttg ttatatagtt catattcatg 63120 gctgaaactt gaccatactc cctagcgctg attgtatggt tttcgtctgg acaccgtaca 63180 ctgcctgata acttgtgcac ctcttaacgc tactatgctc tgggctggag aatgaaatct 63240 ttaagtcacc aggacttgct gtttcagtgg cttgacacct gggccaccaa agaactcgat 63300 cttcatcttt tagggacacc tctgctgcac cttggaaagc caaccttaag tgccagtggc 63360 actttatgcc cagctttgct ttgaaagata tctttcttgt tttttttatc cttctctttc 63420 tctctttttt ttaaaaatac acatagtcaa taggtccagt ctgccctcaa ggccttgctg 63480 ggttttcttc atcatccaat cactttcatt aaaaatggct gcagctgtaa gaactcttgt 63540 ctgataaatt ttcaactatg ctctcattta tctacctgcc ctctgatgct cagtcgtcag 63600 actctaatgc aaaggtggac gtcggctgcc tttgcgtggg cgggcttagt ggtgaggaac 63660 tgatatcaga aaaaaaatgc cttcaagtat actaatttat taataaatat taggtgtttg 63720 ttacttgtgt ggttttggta tatttactac tgaactttcg cctttccctc tgctcaggtt 63780 ctagccctgt tcatctcttg gggaaggatg tgtgttctga aacccttctc ctgtccaatc 63840 tgtaaatcat gaatattatt agcaaaagtc ccttttggag aggtacttgg aggttcactg 63900 cacagggctc tgataagcac tgccaagcct ttcctagtct ttcggagtta aaccttaggt 63960 aacttgatac ctgtaggacg atctgcacac attcacaggc ttctagccaa aacctcactg 64020 tccctgacga actccagagg gcttggtcag cagtgaggcg gatgtggtcc tgtgttggtg 64080 ggatatctca agctcactgc aggttttcct aagcttggac cccacctctg cggaattcca 64140 atgatcaaac gctagcaact ttaacatcct ctttttgtga gaacctggaa gctgatactg 64200 tgtatactgt gaccccccac gtcaacaact gggactgtct tccggtttca ctcatctctg 64260 tgtggcagct ttcaagatga acatccttgt ttcccttccc tcttgttggt gcatgccttt 64320 agtccattgg aagagagagg caggaggatc tttgattttg aagctagcct gggagtttca 64380 ggacagcctg gctacgctaa ctaaaaaaaa aaaaaaaaaa aaaaaaaaag atcacacatg 64440 cgcacacacg aacacacaca tacagagcaa caagaacaaa caagaatggg tcagttatga 64500 tgggggagga aacaggtttt gtcaggttct cactgtgtca gaagatttag aactgagaca 64560 tacaatccat tcattaatta ctagtatgtt aagtttatag tttattagta atttgggaat 64620 tttttattaa taacccaaat ttgtggtttt atattttaca acatataaag tttaattact 64680 ttattggatc tttaaatttg tcttaacggg ttcctattat tatgcccatt ttacagatga 64740 aattgggctt gtaaagctaa gtatatgaga tggtttctta tcactagaac agagtccctg 64800 aaaaggaaga aaagaaattc tttaagggag gaaacgttga tcttaactca tggtttccaa 64860 ggctttaagt ccatggttgg ctggctctgt tgcctttggt tgatggtaaa gcagaaacct 64920 ggtaagaaaa cacgacggtg gaaggttgct tacctccttt cggccagcaa gcagagagac 64980 acaggaagag tcttgggaca ggataactct tcagtggcac aggtccagtg accttctact 65040 agctagaacc ccctccctac cttatagtct attcagctat gaacacatcc atggtcgaat 65100 ataccactag ctgaagccca aacctccaat agatgagcct ttgtggggga cacttaatat 65160 ccgaagcata aaaccaagga atgtgtttaa gccctcactg agaaggttag caaggctagg 65220 tttgaatccg tgttgtgtat gttttgggaa gaagggtaga gcgagtggct gtaatctaag 65280 actgttcatt ctctttataa gatcactgac cagaaacctg ttgccacctg aaacagtggg 65340 cagaaacatg gcagtgaaat ctgtctgggt catgagtaaa gttcaaatgc aagttaagta 65400 agtggtacaa acgtaggcat gagaactaaa cattagcatt atatggtgat cccatgatgc 65460 atagctttcc cagaaggcaa tgcaccatca gctactttct taaagtaccc agagtcaagt 65520 gtgatggatg gcacacacct ggaacccagc actcacttgc aggctggaag accaaggcct 65580 tagggtcagc ctggggagag ggctcagtca gtaaaccatt tgccacacaa gcctgagaac 65640 ctttggatac ctggcacagc aaagtacagg gctctgtggt gcacacctac agtctccgtg 65700 ttgggcagac agagacagga agatccctgg ggcttgctgg ctggccagtc tagctgaact 65760 ggcaagcacc aggctcaggc aagtcactcc atctcaaaaa aataaggtgt aaaatggtgg 65820 aagaaggcat ccaatgtcca actgcagcct ctgcatatgt atgcacaagg tcatacacac 65880 acaaacacag aggaggagga ggacgaggag gaagagaagg aagaggaggg attcaacgtt 65940 agcctgggct acatagtaag accctgtctc aaaacagcca tatagctatt ggagttatag 66000 ttcattgaca tttgatcaac aacactgcag aaactgggtg tgacattgca tatctcccaa 66060 ttctttcact ccagaggtga aggcaggaaa gataaaaagt tcaaaatcat ctttgggtgt 66120 atagcaagtt ggagaccagc ctgggacaca ttagatgctg taaaaaaaaa caaaccaaac 66180 aactgaaata aaacaaaaat tcagcataaa atactaaaat atgtgacttt ctgattaaag 66240 ctaatgatag caccatttgt accatagaaa ggtcttctct ggcttgcgct tcttcctctg 66300 aggagtatag acagctgaaa aggaaggctg ctagtgtgag ggcagaggag acaagagagc 66360 aaccagaaga ataagaaaca gaagcaactg ttgggagatc caagccatgt tatgcaggca 66420 ggcaggagaa ggagtctgtt caccaatttg aaggactaag aaggagtttg tgacccctgg 66480 gaaaagacta cagactcgat ccaattataa tgaaaagatt tgttgattag ctgctagctg 66540 gatgagtaat ctctgagcca acttcgagat tacttcgcag aagggttacg gggaaggact 66600 ttaaaaggag aatatcacac aaagaccttc aatatctagg caagcaagta acaactgttc 66660 tattttgaca aattaagtga ttgaggcgac tcaaaacaat cttgggttat ctgtgcaaca 66720 agttacagag gcaaaaaaaa aaagttagtc ataacaagtg ttatgtatgg ggaggaaggt 66780 gtgactgagt cagggttatt ggaaatttat cttccgggga atggagttag agactagtgg 66840 gtatggaaat gaatgcctag cataaaacgg agcttctttc attttcttca gagagtaata 66900 tagttcctgt gggctgtttt aggctgaact caattagata gggctgcttt tcaaggagaa 66960 actgcttcaa ggagaggaag gagctgggcg agcatctgtg gttctccaac tttcatgtaa 67020 ataagtcacc cgggagtgag cagcttgttc tctttaaaaa aggctgattc ccacaatctg 67080 attggagagg tttgtggtga aattcaaaaa tgaagccctt gcaggttttt ggaccacact 67140 tagaaaacta tctttagaga atttccgttc ctgccaatgg ggacaagaac agctaagagg 67200 agctggttag actcgcacct tcagaggcct cctgtctacc cttttaatgt gcaaatgaga 67260 tacctgaagg tcctgctcac aggcagactc tccaggcctc ttctgggaaa gggcagctgg 67320 catcctgtgt tgctgccaag ctcttgggta atacaggcac tgctgggttt ttctttttcc 67380 ccgtgaaact gggtagtctg ggatgacttt acctttcagg aaaggggcag aacaaacaca 67440 ggagtcgatc actcatttca caggagggaa ggaaacacac tttgatgagc tgaagaaaca 67500 cccagaacca agcaaatctg gtcaaaactt acaactggtt ggggctgggc ttggtaaaag 67560 tgcctgcagc agaagcacgg ggacaggagt tcggatcctc agcatccatg ttagaaaata 67620 aggtaggtgc agtgctgcat gccccatgca tgctcccagt gctggggagg cagggacagc 67680 agaatcccag gggtttgttg gcttgctagc tccagggtcc atgagagtct gtgcctaaat 67740 gataataagg tagaaaggtg atcaagaaag acatagaact ttgggcacac aaagcctacc 67800 cctcctccag caggattgta taacagcagc agaaagaaag gagacttaca gaaagaaagg 67860 agactcctcg aagcccaact atttggcatt tgtgcagaga gctctaggga tgtagctttc 67920 aagggtggac ttcttcacga gcagttgtat ttgggttact catacttctg taagtaactg 67980 caagaaattc atctggacgc aagaagcatc atttctttgt tccgttggca gtgccctatc 68040 tgaggcaaat agacatttgc tgtgtctctc caggaaaagt cacacaatac acagtgaggc 68100 tgcctggcag aggaatcctg tttatcttgt ggtcaaccag aataaaagag acaagaaagg 68160 ccagagtctc agtataccct agaagaatat ttccccaggt aatcaacttc cctccattag 68220 gctgcacctc ttaaagtgtc tgctacttct aaatagtaaa ctggtcacaa agattagaca 68280 catgtccttt gggactcact tctgaactgt acccctgggc tatgaattgg tcactttcct 68340 gctgttatga caaaatatca ccactcaaga caatctaagg aaaaagaatt tgagcttcca 68400 cttttggtga gagagtccat aaagacaggg aagagatggc agctggaaca ggaagctaag 68460 cctcaatcaa gaagctgaga aagtgagctg ggagtagtga ggctatataa catttcaaag 68520 tgatctgcct cctccagcaa ggttgtacct cctcatcaaa cagtactacc tacttggtcc 68580 catcttggtt gcttttctac tgctgtgaag atcatgacca aggcagctta taaaaaagga 68640 agcatttaat tgatcaattt ataaacactt tataggggac tggaaaggtg gcttagtggt 68700 taagagtact ggctgttcct cttctagagg ttctgagttc aattcccagc accccatggc 68760 agctcacaac catgtataat gggatctgat aacttctttt ggcctgaagg cattcatgaa 68820 gacagagccc tcatatacat taaataaatt ttatgaacag cttataaaaa cgttataaaa 68880 cataatttca gagttaccct atgactacta tggcaggaag catggtggtg gcaggcaggc 68940 atagccatgg agcagtagct gagtgcttac atcatgcctg aaagatgttg acagagtgag 69000 acagggcttt taaaaacaaa aaccacgttc cctgttatgc acctcctaca acagggccac 69060 acctcctaat ccttccccaa acagttccac caattagaga tcaagaattc aaacatgcaa 69120 atcatgctca ttcaaaccac cacagagcct acaaggctct cattcaaagc accacaagac 69180 agcaagacct atatgaatca gacctgaggt gatggctgta gcttcctgag aaagggtttg 69240 gggaatcttt ccaaggagcc agctttgctc tgtcccttac aagtagctgt ctccccagct 69300 ctctaggatc ttcctagaga ttagattagg atttctccca aacaaacaca aaaaaagtcc 69360 aaatggagag ggttcaaact caacatttaa aaaaagtgag tcacagtaat tatgttgggg 69420 ctcaaatatc tatttcttag gatagaaatc tctcatattt aaggtctttg ggaggagaaa 69480 catgaaaaat ttctggagtt gtcatgcata cacacataca catacacact atatatatat 69540 atgtgtgtgt gtgtatacac atatatacac acacatatat gtatatacac acatatgtct 69600 atatgtatat atacatacat atatacacac acatatatgt atatacacac atatgtctat 69660 atgtatatat acatacatat atacacacac atatatgtat atacacacat atctatatgt 69720 atatatacat atatacacat ataatattta aatatgtttt atatatttat atattataca 69780 taaatatgct taagtattat ctatttaaat atatatttaa tatttaaaac atatattata 69840 ccacacgcag tcctctaacc ctccattgga gatgtgcctg ttccgtgtta gttagcattc 69900 tcctactcag tcaccctctg cttcatccac tgcataatgt caaatgggag atctcacaga 69960 gaaagaacat gggacagcaa gttcattttc aagatcacat gacagttatt tccataagta 70020 tagacacgtg gatttcacat ctcgtttcgt ccatttgttt gaatgcagca tacctgtagg 70080 acggtgaaag gcagcctggt tcctggttga gctaaggcta gaaaccctac tgaccctaaa 70140 gggatgcttg gagtttgcct ggttcttagg taccaggctt catgcacgag gccacagccc 70200 tccatagatt tgtggccgtc agtaacgtaa gggcaacacc gcaagccaaa gccccccccc 70260 ccacctgcca cacacacaca ggtagaggac gtgacccatg gtcacctagc ctcaagacac 70320 atctctattt taatgaggta cctaaaggcc tggaggactt agccaataaa ctctccttcc 70380 cagatgttcc ttcctgcaaa aaaggtattt aacttcaggc cctccctgag aagtggggta 70440 cggttttact cagccacctt tcaccattac aataaatgcc ttaaaatcat ggattgctac 70500 ttttcaccgg gatctgctat ggagaagacc ttctttgcct acagagctac catctaattt 70560 cttgtagaag acctctctgc tctcccagcc atggctgcca ccaagctcaa gcttgctgcc 70620 acagacagcc agagctcctc ttttttcccc ctcagccctg ggctagattc agcccgctgg 70680 cccccactct gttctcaggt cttttgtggc tctcagtatg cctgggtgtc caagagcccg 70740 agaaccagac agctagggcc tccttgtagg ccgggggacc cccaagccag ttctggctgt 70800 cctgggatct cagactgtgc tttcccattc tggaagggtt attccatggc tccccataga 70860 ccaacaccag cccggtgaca gtgtggggta agctctgtca aaactccagc gttctgtctc 70920 cccaagtagc tgtgctctgt ggtgggcacc cctacacaca cccatccaca gaaacacaga 70980 tgagttcttc cacttagtct gagacatctg aacagctact aggttctggg ctaggggcta 71040 gacgtgggga gaatttgatg gagcgttgaa actgctgatg aggaaagaga agctctctgt 71100 tcacggctct gtcctggaca tccagagcaa agtatagaaa tgtacttatg ctataggctg 71160 aataactgag tgaatgcaag ctcctgactg caagaagttc ccaacatagc aagagacacg 71220 aagagagaca ttcttataac aatggtaagt gatgagaaat atggaaacag taattaatta 71280 tatccaagca taatggttac attccataga agcatttgaa gacccagaca ccaaatacaa 71340 ctcaaagaaa agtgatataa aattttctag tgtaagccta gtaatttcaa tgataagttg 71400 catcaaaagt caggaactga tcaatgcgat tctatcctct tcagagcttt gaccacgata 71460 agaaatagct tgatgccagc ttaacattag actctaaagc agtgaccttc ctagtgctgt 71520 gaccctttaa aacagttcct cgtgttaggc tgacccccag ccataaaatt acttcgctgc 71580 taattcataa ctgtagtttt gctactgtta taaatcataa ggtaaatatt gatatgcaac 71640 cctccagagg ggtcgtgacc cccacgggtt gagaaccagt gctctaaagt gaactgcagg 71700 tgtgctgtgg gctctaccac ataaacaagg atgccatagc ttttctactg ttgttgttcc 71760 ccgtttccag gcttatcatt aaccatcatc aggagatacg aggcccaggc atgtgacagc 71820 ttagcagaag acaggccaag tgcaaccttg tgtggagctc attctctgag caaatgttca 71880 aaggctctgg gggtgctcat tgctcagtct cactttgaag cctgtactat aggaacaggc 71940 attatttcca ctctgttgat aaggaatctg agacatgcat tgtgatggcc ccagtattat 72000 acagctagca aagggtggag ggagccagga aggacatttg tagagctgag tgactcaagg 72060 tcataaaatg gcaagtggca agtggcaagt gaggtgactt gactcatacc tgcctggaac 72120 cttttgtctt gactcaacca aactcctcct tattgatttt ttaaaaaagg tatcttttta 72180 aaaaatgtat gtgcatgttg cactggctgg ttctgtgtgg cttgagttat catagagaaa 72240 gatgcctccc ttgaagaaat gcctccatga gatccagctg taaggcattt tctcaattag 72300 tgattaaggg tgggagggcc cactgtgggt ggtgccatcc ctgggctggt agtcctggat 72360 tctattagaa agaaaggcag gggagcaagc cagtaagcag cacccttcca tggcatcagc 72420 tcctgcttcc tgacctgctt gagttccagt cctgacttct tttgctgatg aacagtaatg 72480 tggacatgga agctgaataa accctttcct ccccaacttg cttgttggtc ctgatgtttg 72540 tgcaggaata gaaaccctga ctaagacaaa cgtgtctgta cccgcatgtg tttatgctta 72600 ccatgtgctt gcaggagccc atagagaaca gaagagagct tctcaagcga tcctcggaaa 72660 ctatgagctg tctgtgggtg ctgaaaactg aacccccatc ttccataaga gcaataagca 72720 ttcttaacta ccagacaatg cctccagccc tttctttttt ttttttatta cgtattttcc 72780 tcaattacat ttccaatgct atcccaaaag tcccccattc ccctacccac ccatttccac 72840 tttttggccc tggcgttccc ctgtactggg gcatataaag tttgcgtgtc caatgggcct 72900 ctctttccag tgatggccga ctaggacatc ttttgataca tatgcagcta gagtcaagaa 72960 ctccggggta ctggttagtc cataatgttg ttgcacctac agggttgcag atctctttag 73020 ctccttggat actttctcta gctcctccat tgggggccct gtgatccatc caatagctga 73080 ctgtgagcat ccacttatgt gtttgctagg ccccggcata gtctcacaag agacagctat 73140 aacagggtcc tttccgcaaa cgcttgctag tgtatgcaat ggtgtcatcg tttggaggct 73200 aattatggga tggatccctg gatatggcag tctctagatg gccgatcctt ttgtcttagc 73260 tccaaacttt gtctctgtaa ctccttccat gggtgattgt ttccaattct aagaagggac 73320 aaagtgtcca cactttggtc tttgttcttc ttcagtttca tgtgttttgc aaattgtatc 73380 ttatatctca ggtatactaa gtttctgggc taatatccac ttatcagtga gtacatatca 73440 tttgagttct tttgtgattg tgttacctca ctcaggatga tgccctccag gtccaaccat 73500 ttgcctagga atttcataaa ttcattcctt ttaatagctg agtagtactc cattgtgtaa 73560 atgtaccaca ttttttgtat ccattcctct gttgagggac atctgggttc tttccagcct 73620 ccagcccttt cttaatcact ggactatctc tccaatcctt ccttattgac atatgtttaa 73680 aatgtaaagt atttttgtta tgtgtgtatg caaatgaatg tgctacaaaa aggatgtgga 73740 caacattgaa gaattggttc tttacccacc caaggatcaa tcccaggctg tcattgactg 73800 ggcttgctgg ccacttagta aaaaaaaata ttacatttgg taggaaggct ggctatcctc 73860 ggtctaatga acttcctgac ccaaccatcc cagtgatata ttatagacat acatcaccat 73920 gtctgacatt gatagttgga ttattttttt taattttgaa ataagttata acacattaag 73980 tttaacaata ctataacgtt cacattttct tttccaccag tttcccccaa gtaagttagc 74040 cttagacata atcaaaacca ggagattgac attattatat taccaagcaa agacggatgc 74100 taggtggttc acgctggttt cttgcacaca cttttctgat gtattttaaa catcagatgt 74160 actgattcct gtacccacca ctaccatctg gaaatagcac tacgaaatgt ccctaaggac 74220 actcttgttc acaattgtac cttccctttc accaccagac atggcaactc ttccacacag 74280 ttgtcactta cagagtctta catcaatgaa atctggggtg aatgtgccat ccaaagaaac 74340 acaggaacca gagttgggtt ccaggaaccc atgagaaaaa gcttgggtag tagcatacac 74400 tggtagttcc agccctggga agacagaaac ggtgaaccac tgagatttgt agcaagccaa 74460 cgctaccttt tctcacattt agccctactt ggtgagccca ggccaatgag agaatctgtc 74520 tcaagaagca aggtgcgcga catgtgagga agaatactca aggtctcctc tggtctccac 74580 atacacacat gtacatactt atgtacaccc acacataaat ttgctcctcc ctttggacac 74640 atgtccagga gtgtttagaa actggatcac ataccatgta acctccagag actggctttt 74700 ttctcattga tgtgaatgta gcatagttta ggcatcctca cgctgaaggg cttttggatc 74760 atttttaatt gaagctattt acaaataaca ctgcaatgac cacttaagaa cagtatcgac 74820 atggattttt atttctttga caaatatcca ggagcaggat cgctgggagt attagttact 74880 tttataacac accgcaacaa aatcagcttt tgggagggtt tatatggggc ttataaagga 74940 atgagtccag caccatcatg gtggggaagc atgtctggag gcaggaatgg tggctaaaca 75000 gcagctgaga gctcatgtct tgaacaataa acaagaagca gagaggctaa atttgaaatg 75060 gcacaaatct tgaggtctta aagcccatcc ctagtgatgt attcctccag caaggtcaca 75120 cctactaagc ctccccatac acccatcagt tagggaccaa gtatttaact gcccagacca 75180 tgggggatat ctcattcaaa ccactacact ggataaaggt aagcttaatt taaatgagaa 75240 atgcccccga caggctcagg cattgcacac ttggtctcca attgtgggca ctatttgggg 75300 aggtttggga ggtatggtcg gtcctgccat aggaagtatg tcactggggg agggcttcga 75360 gaatatataa ccccgctcca cttaccattt gctccctctc tactgcttgt ttgtggttgg 75420 agatgcagtc agtctcttgg ttccctgctc ctgccaccat gcctgtccct tgcagacgga 75480 ctcttctcct tccagaccca aaagctaaaa ataaactctt aattccataa gttgttcttg 75540 tcatgatatt ttgtcacaac aacaggacaa ttacacccct ctctccccaa ggttatataa 75600 atagtaagga cctctgtgga gacaggatta tttccagtct gttgtgttgt ctgcaaatca 75660 tgtagagagc tcttgggata cacccttcat gagtcatcac ctatactcag ggtttttgag 75720 taatgcagta ttgtacttgt attgtccagt gttcctgtca gtaagttctc acccataatt 75780 ctgtaagtaa tccagataaa cacattggct tttgaatgga cactttggta ggatagtttc 75840 tttagtctgt cattggtgct ttatctgggc tgagtagatg tttgcatgaa gtctacactc 75900 tttgtttttt gtttgtttgt ttgtttgttt caagacaagg tttctatgta tagccctggc 75960 tgttctggaa ctcactttat agaccaggct ggcctcgaac tcagaaatcc acttgcctct 76020 gcctcccgag tgttgggatt aaaggtgtac gaacaccacg cctggctgaa gtctacactc 76080 ttataaaaaa aatagtcctg ttctcacttc atttctgttg ctgtgataaa acaccttggc 76140 caaacagaac ttggggaagg gaaggggttc attatggctt acagttccag gttctggaca 76200 gtcgatgaga aggcaagctt agtcacctca cacgcatagt aaaggacaga gaaaaatgaa 76260 tggatgcctg ctgcctgctg cctgcttgct gactgcctgc ttctgtttat cccctgccat 76320 aaaatagttg agaaccttct gcatagagaa tgttgctgcc cacactgggc tgggtcctcc 76380 tacatcaatc aacaatcaag acagtctccc acagacatac tcacaggatc taggcaattt 76440 ctcaaccgag actctcttct caggtgattc tgggttgtga caagttgaca gttaaggcta 76500 accatcacag tgtatattag aacttatttg ttctaataaa cttagtaatt cccatgggtc 76560 attagctcta cctggtagga actatctttg tacactttgt taatacaaat tgactacttg 76620 ttttaatacc tcatataaaa tgacagctgt ggcatttgtc tttctatgat agagttattt 76680 cacttagcat gtctttaaag tttatctgtg ttctcatgta ttacagaaat attcataatg 76740 ttaaggctga ataattttca tgtgcatgtg tacagacaca tgcacatact ttctttgctc 76800 atttgttatg agggatcata caggttgttt ccttaagttg gctactgtga atagagctac 76860 catgaagatg gggtgaaaat atctgtttgt gatatttaga atggctcttt tgggtaaaat 76920 tcttttgaat actagatcat gtaagagtcc aatttttaat tttgaaaaaa tatttatttt 76980 tattttgtgt gtatgggtgc tttgctagca tgcatgtata ccacacgtgt acagtaccta 77040 cctgcagagg tcagaagagg gtgttagatc ccctagaact ggtgttacag gctgttacga 77100 gctgctttat ggtgctggca accaaacgta ggtcctctag aagttcagac agtgctctta 77160 gccactgagc catcatcttc tggatgtatt tttgagaacc tactatactc ttctgacatg 77220 gcatatcatt ttacattctg tcagttgttc aaaaagcttg gagtttcccc atatactcat 77280 taatactggt ttatcaccat catcatcatc atcaacaaca acaacaacaa caactttatt 77340 ttataatggt aattgtgaca gatgtaaagc aatatttcat gttggttttg atttgcatct 77400 ttcagatgtc agtgactata tcctcgtggg tcactcatat gacttcactg tagaaatact 77460 ttaattattt aaccccctat tcctcccctc tccacttttt atagtaatga gggttgaacc 77520 cagggcctca tacatgccag gcaagaacat tacccctgag ttgtatcagt cagccctact 77580 caatttttca gcctctttta acatcaagat agtaaatgtg ttgctgtttg gaaagtttgg 77640 ggattttatt acatcttggg agttccttat atactttgat aattaacccc ttagtggctg 77700 gatggtttgt aatattttta tcctttttcc tgagttgtct cttttttctg ttaatgtttt 77760 ctttccttat gtgatgctat attggtctga atgagaagtg ttccctgcag gcctgagcat 77820 ttgaatgctt ggttcctgtc ttagtcaggg tttctattcc tgcacaaaca ccaagaagca 77880 agttggggag gaaagggttt attcagctta cacttccaca ctgctgttca ccaaaggaag 77940 tcaggactgg aattcaagca ggtcaggaag caggagctta tgcagaggcc atggaggcat 78000 gttacttact ggcttgcttc tcctggcttg ctcagcctgc tctcttatag aacccaagac 78060 taccagccca gagatggttc cacccacaag gggcttttcc gccttgatca ctaattgaga 78120 aaatgcccca cagctggatc tcatggaggc acttccccga ttgaagctcc tttctctgtg 78180 ataactccag cctgtgtcaa gttgacacac aaaaccagcc agtacagttc ccagttggtg 78240 gtgttttggg gggaggtttg gataccacaa gcttgttgga gaaaatgtat cactgggagt 78300 aaactctgag aacagatagt ttcccacatg tctagttcat acctgctgct ttgtgcctgt 78360 ggtccgagac atggctctca gatctctgct cctttcacca tgcccgattg ctgccgtacc 78420 tcacaccatc atggacactt atccctctgg aaatcatgag gcaaataaaa tcttcttcct 78480 ataaatcgcc ttgtactcgg tattttatca cagcaataga aacggaacta tgtagatggg 78540 gtccctgttg tctacttttg cgtctgttgc ctgtgcattt ggtgatgtat ctatgaaatc 78600 gtagccaaga ataaaggcaa agctctttcc ccatctgttt tcttctgaag ttttatcatc 78660 tggggcatat ctttaagtct ctaattcatt tagagtttgc ttctgggcat aactcttttt 78720 ctcaatatgg cagtgttggg gactgaaccc acagtcttgt gcatccctgg tgagtactac 78780 ttcatttaat gtttagagaa gccccgacta ttccctaacc tgactctcct tctcagtcaa 78840 gtttttattg atggacgcag ctccagtttg tatcagagaa tgaaggttag cacagtatct 78900 gttttcatgg aacttctggt aaaatataca tccataggcc aatatacatg aaaaacaact 78960 acacccctga ctgccttcac caacctattg ctggtatttt atttgtgata gttggaggta 79020 gggtgtgtgc ctgccactgc atatgcgtag aggccaaagg acagctttgt gagtagttct 79080 ctcttacctt tcttagcttc taggaagtga gctaaggtct gagggcttgt gtacaagtat 79140 tttatttgct gacgcacctc accacccttc attaccaaca tttcaatgtt ccccagctgt 79200 atattaaagg gcccaagaag tctataatgc agtaacctgc ttaacatcac tgacacagaa 79260 cttattaaat ttgcatgaca atgtaaaaca tacggattag tatataattt aatttaaaga 79320 aatcttaatt tgcttgcttt tataaatgca atttgttaaa ttgcctcatg gaacagctat 79380 tttcttcaga ggcaaataaa agagacacta atattggtaa tcctgcttca cccaggaaga 79440 gcactggtcc tagtcaggag tccagcaccg ccgtcactgt tgggtatgtc tttacattta 79500 agtcaagttc agagttcaga gtaagagact atcccagatg ctagggtggc tttgagggtt 79560 ctgagaaccc aaaggtcacg tgaaagatat ggttactatt gtcaggctga ttctaatcag 79620 aaatcagagt ctggagatgg agcagatacc attgagaagc agttaggagg tggtaatgtg 79680 gcactgacaa cccagccaag gctcaggtag cggtggacat gcgcatttgc tttctcttgt 79740 gtttatcggg caacaagcct gggtgaactg gaaagcggat gcggtctgaa ggtctgcatg 79800 aaaccacaga tgtcgatccg tggggggcag actccggggg catgtgtctc aggggtatag 79860 cacttggagg tggcaaggac taatttcaag gtctcacact ttaaaagcct aaagtccaag 79920 cataggtggg agcccagcct agactcagag cgcagacgcg gacagagagc atgctaggag 79980 cctctgttgg acagtcaaac atgaccgagt aagaccgaaa ctccatagag acagactgag 80040 ggaagggaga ggttaaagac agggaacctg ggctctaaga tctccctgta acagctctcc 80100 ctttgtaaga ttggatcgaa tccctagggt aaggggcaca gctgagcttc caggtgagac 80160 aagagtaggg agaagaacca tcaggtaaga caatatagtt agagccccaa accaatgaac 80220 caactaacca accaaccaac caaacaaaca aataacccaa agaccaaaaa aaaaccccta 80280 aaaatcaaaa cccaaaccaa aaaacctcaa aacacaaaac aaaacaaaaa gacccatgag 80340 ccacaggagg aacacagttc tgtgaaccct gggctcaaac aagggtatgg atagaaatta 80400 aatgttatat ggatggaagg ggacctggag aaatcccagt gttttctctt ggatgaagac 80460 tcttgatccc tgaaactaag tctcacaagc ccagatgaag ttgtgagttt aaatcttgtc 80520 aagtgtgaaa ctagagccaa cagtcaaatg cctccctcca ccagctaaac cagtccattt 80580 taacctgtgg attaaattgt ggtttaattt taggccagaa aaaaattttt acttgccata 80640 aatttaagtg ctttagatac ccatgacata aagactggag cattatgtaa aagggaggca 80700 cattagaggt catttaatga cttgtgccac ttcagagctt tctatcacat agtccacagc 80760 aattcaaagt catgacccct agaactaaaa acattaccca tcagcctttg aactctgaaa 80820 ccggccaagt gtaataagcc cagagcaaag tacagacttt ccagtgactg agcacaggca 80880 gtgactagac cagatcggcc atagtgagac cacacactgg ccctactcag cgggcattca 80940 ctcagcctag attccatttt ggttgctgct cacctagtct caaaaagtct cagctaagtg 81000 atgtgacagc cctttgtgct aggacatgat ctgttctaaa tatctttcca caggtgtgct 81060 gcaaatcacc gtcaacctga taagaacaat ccctacaata aagtgagtta tttataccaa 81120 catcctccca tgttgttgaa ccaatgaagc aaatgtggaa gaataatgtt tggctccttt 81180 ctgttttgaa aacattttct gggaaagcag gtgtttgcag actaaataga tatgcatgcc 81240 atgggtgata gatagtctta gatgtcaggt cagttcttca tcggggacag atctttggag 81300 gacactgtaa tattggtcat gacagaatag attctgatag aagttggtgt ctaaaatgaa 81360 aatggcttgg agaccacagg tgatcgggga gttcctcatg gctgttactt ataggaagaa 81420 ttcttccctc ctataagccc tgctccatcc agctcccaaa gatcaaccag tattaagact 81480 gacgttgttt aaagcaccac tatctgtccc tgtatctact ccaacgcatt gagccagcta 81540 tatagcactt tttttccgtt ccccaataac cctaaatttc ttctacttct tgttggagat 81600 gtttcctttg ccccagatgt ctttcatcca tccactaagt acttccatcc cttcctgtac 81660 tagctaattc tttcctcccc tccaagactc atcacaccac cgtgccctcc aggatggctt 81720 ctctcagtcc tcactcatgg actgagaggt gctcccacct cacttgcctg gtaggtgctc 81780 ccacctcacc tctgcatgcc aggagctaag aaaaggaagt tagaacatta ctagagattc 81840 gcttggaagg aaggcaggaa ctgagatgga aagatggggg gggggggaat gataaaccat 81900 atggagccac atgaaacctg gggttgtgtg cagtaagggg tgaaaaggga tctggctaac 81960 tgggtgttct caacgcaagg aaagttcata agacaaaccc aaatgagacc attaatactg 82020 ccaggtacag ggcaatgtct acaacaacga cgatacagaa taacccgtgg cctggagagg 82080 tggctgggta catagagtgt ttgctaagca aacaggagga ctggaaacca gaactttagg 82140 atcatataca ttccagatga gcctggcagc ccaccaataa taccaatact aattaggcag 82200 aggcaagggg agctccaggg caaggtggct tgctgaacta gcaaaatcag cgtgctccag 82260 attcagctgg aaaccctgtt tcagtgaata agatggaggc aataagacat ccagtgccaa 82320 tctctctttc ccacatccac atttgcttgg tcacacagga gtatccacat ccatccacat 82380 gcatgcatac acagccccac acgcatacac atgtgggaga aaaggaaaaa ggaaagaata 82440 aagtaggtag acatgacgtc gagaagcaat ttaagcaggg tggatgtgtt ttctgtccat 82500 cccttgtgtg tttgtttctc tttcctaagc tccactgttc attaggggaa agttcaactc 82560 acataaactt ttcatgtctt cttcagatat cctcaccttg agatgatgta gtgagtaggg 82620 tcttgtgcac aaatccctca gggtttggag tggggtatgg gtgtcaaagg gcacaggtga 82680 aacaaacctg gctgtgcact gattcttgta gggtctaggg ttttatgggg tgcttctact 82740 tttgggggga cttgacaatt attcacaaca aaactacaaa acagcgcatc aggaactatg 82800 ggttcttctc aagtggaggg cattacatat taactgaatg cgtacacatg cctttaagat 82860 gggatatgca tatgcactgt ctaagatacc ccgagaaggc actgggtatt tcagatgctg 82920 aacacagatt ttcctaagct cttgccttca ggccaaattc tgcttttcct ggactatggc 82980 actgtgcaga gaaaaattat ttgagggcaa ggtggtgaga agtcttaaag gccccatagc 83040 cgaggatggg gtagggctgt ctcttctcct gtgatcactg aaggcctctt atattcaaca 83100 gacaccaaat ctgagaggtc ctaccatggc caaggccata tcccctttct tatgttcaga 83160 gatggaaaag gaggataatc aaagtgctta actcacaggg tcattagtac catgaatgtg 83220 ttaaggttta gtccagtgag ttgttagcat cgggcatctg tgctttacta tcagagttag 83280 tgtttgcgtt ggagttagtg ttatgggtca atagttacca gttagtcaga cacacactat 83340 gatggatagg ttgtttatag acagagagaa gacatggttt tctgaatata agacggggaa 83400 ggagtaccag ggctaggaat gagcagagaa actatttgag gatgcagctg tgtataattt 83460 aggcagggtg tgtaggggag gggagagatg tgaaccagga agcaaggcag ggctggatgg 83520 atgatggtgg gcctggcatg gggcagcaaa gttgagactt gaagcaggag gccccgagga 83580 gccagggagt gacctggtcc agggaggggc gagatgaaaa catacttcag gaggattagg 83640 ctggcatgag atataaagtg tctcggtttt gggcaggttc agaccaaggg tcgagaagtc 83700 cctacagagc tggaaatttc tagacttcct ccagcctcag gctccaagct acatttttgc 83760 caggctgagt gggaatcctg gagttttcca tgggacccaa caattcatct ttgtgtcttc 83820 tgttaccctc agctttgttt ttgtgttgcg taacatttcc aggtctcaat atcccaagca 83880 agctggagtt ttcagaggtt aaactctaga acctacccag cctgggtggc atctctggaa 83940 gcaaaaagag acacccccct tttgaccaga gatccttgta gacaaatggc cttagctgac 84000 cctggctgtt ctttggttca tataacaata ctcctctcct ctctctagtc cttctcccaa 84060 cctgtttaga cacaagactt acataaacct tctccactcc cctctgcttc ttcaggtgga 84120 accttgaaga aaaaaaaagt tgttactagg ccaaggagca aggtttggaa agacacgatg 84180 tgctgccaaa tccaggaaag caagttaatg agaaggtgag actctgtcca cccactttct 84240 ttcattggcg gaatttttag gtaacaaaca tagctccagg caggtgtctg tgccccactc 84300 ccatgctgag caggagctgg acccgagctg caggtcccac tcagccattc agtggcttca 84360 tctctgcacc atctccagac ccctatgtcc aattgcacag cagtgatcca ggtccttccc 84420 tctcgaagtc cttttgagct ctagctttcc aggatgccct tctacgggtc agtgtgttgg 84480 tctggatgag tcctgggtct gaccagaact actctgaaag ggaagataaa gaaagagcct 84540 tagaagaact agctgtccta acaacaggag accccgtact cacatactaa tgtgcactca 84600 tgagctcatg tggaagtata catataagca acactggtga gcacatgcac acacacacac 84660 acacacacac acacacacac acacacacac acacagcatg catgcatgca cgtaaaataa 84720 agcttataaa acaaaacaaa acaacaccaa gaagcaagca agcaagcaag acctagggtt 84780 tttatacatc tgttgtggga ttgttgttgt tggtcttatt gtgttttgca tttttggttt 84840 gagacaggtt ctcactatgg aaccctggct gacctgggga ctgcctgcct atgcctccca 84900 gagtgctggg atcaaaggtg tgtgccaccc atgcccagct gctgtggatt ttataggtcg 84960 ttgatggctg ggttttggtt atgagtttaa cactgctgtt gaatgcagag agccttttaa 85020 atcgagcatc aatgaggggt tgttttgtta ctgaggggct ggacatgggt ttgcttagga 85080 ctgagatttg ctttctgttg cctcccttgt tctgagcgca attgcttagc tagatgtcga 85140 tggcctttca ccatgggttt cctgtgagcg ttacaagctg agattggcag gtgcattgag 85200 gaggtgggca aggggagggg cgacagcaca atgctgtttc cctattggtg ctaagtgctg 85260 ttcctgttta ataagtgcag ttttgaactg gaagagagga ggtgtgaaaa caccacacga 85320 gtcccctaaa atagcctctt aacaaccaaa tcaggagtag aatctggtct ccgttcagga 85380 aacagggtgc ttggatgtcc actcccctgg ggctccttag cccgtctttc cccttaggtt 85440 caaaggttct tcataacctt tgggtccatt gactttactt ctgataatat caatgacctg 85500 gggacatcca ccttacatat gaaggacccc tgtcggcatg agtagttgtc tattaactgt 85560 caccatgtag tcagcttctc tggcagagat gattacaatc taagtctaga atgctgggac 85620 cctttaatac agtttctcat attgtggtga cccctaatct tttttttttt tttccgagac 85680 agggtttctc tgtatagccc tggctgtcct ggaactcact ttgtagacca ggctggcctc 85740 gaactcagaa atctgcctgc ctctgcctct ttgttgttac ttcatacctg taattgtgct 85800 actgttatga atcataatgt aaatatgtgt gtttttgata gtcttagccc ctatgaaagg 85860 gttccttgaa ccccccaaag gggtcgtgcc ccacaggttg agaaccagtt ctctaaagag 85920 ccatttatga ccccctcttt ggctatcagg cttctaaaag aatttcccta ggaatctcca 85980 catgggagaa gaaatagcca aaaccccagg gacatccaga ggaagaccta atgttttgtg 86040 aagaattttg caacttatta gatgacttca tagagagttg gcgctgagac ggccgtgggc 86100 agaggccaca ccttgtctga actattcaac tatgcatatc ttttcttttt ttatgtaccc 86160 tcacgtatgg aatttgaaat caaacttgga tgtagtggtc actgtatttc ctttgtccct 86220 ctttccattg tggccacttt gaataaatct ccattttaat ttggctcttt tatttggctt 86280 gttcaggata ggtggccaaa cctggctagt tgggggctca ggttgaaacc ctaatctggt 86340 aatagtaaca tatgtcctga gtctggacct gtacagccat gctcagtaat gaagaacaag 86400 cagcatattg gatctctcct aaggggtgaa tttcacccct gctaaataag caataattga 86460 tcaaacaagg actggctgca tgctttttat gctcctcagt attcgggggc agcctcctct 86520 ttttcactac tccaaattct cattctgact tgagcttaga gttgtgttta ctccagactc 86580 acgcggtttc ccttgattat tattgtgtgc ctctccctct gaagctgaag aaatagctga 86640 tccttacgtg cttgtggagt aagcaaagaa gtcacatgac tggatcagta caaaaatgta 86700 ttccgctttg ctgtagtcca gtgaatttga agccatggat attttccctt ttaaatgtac 86760 agttcatgtt tagagaaagg aacgcaacga tgtatcaatt gcttgtatac ttactgtcaa 86820 agtagcagaa gttaacattt aggctcagta cttttgaatt tccttctttc ttgaagaaat 86880 ggagcattac attcatttcc atctctactg agaaaaccag ggctgagtgg ccagccatgt 86940 gcttgctgtc taaaatgcac tgatggggac tggagagatg gctccatggt aaagtgtttg 87000 ttctgcaagt atgagatctt ctgtctagac atccagaaca acatctaaaa atatctagat 87060 gtgatggtgc cttcctgtaa ccccagcagg ggtaggaagg gaacaagtgg atccttgggg 87120 ctgtctgatc agccatttca aagaatcagt gagatccatc atgaatgaga gaacctgcct 87180 caggaagtaa agggagaggc tggagcaata gctcatggtt aaaagcactg gctggttcca 87240 gctctagcag caatctacac ggtggttcac aagcctctgt aaccctagac ctaggatgta 87300 atgtgccctt ccagtctcca aggatactgc atgcatgcat ggtgcacaga cacacatgca 87360 agcaaatacc caggcacaaa ttaataataa tgatgatgat gatgatgatg tagatagata 87420 gatgatagat gatagatatg tgataaatag ataatataga tgacagagat aaatagataa 87480 taggtgatag atagatacct agatggatag atagagataa tatagttaga tagatatagc 87540 tagatatagt atagatagat aaatagatag agatagataa atagatagag atagataaat 87600 agatatagta ttgttcaata gagatagata atagatagat agatagatag atagatagat 87660 agatagaata gtatagtata gtatagtata gtatagtata gtatagtata gtatagtata 87720 gctagctagc tagatatgag tgtggtagca catctgggag gcagaaaagg tctgcaagtt 87780 tgaaggcagc ctggattaca aagcaaattc caaggctaca tagtgagaca ttatctttta 87840 aaagtacata tataaagtaa agcagaagca gaaataagga aggcactgcc cgttggcttt 87900 tgtccacata cacgtacaca ctcttactca catgtgcaca catataacca gggccataca 87960 gaatacacac acacaaagtc taagaatcta gggccaacga gatgtctcac tgggtaaggg 88020 cacttgctgc tgagcttgat gacctgaggt taagccttga gaccagctct cacagatttt 88080 cctctcatct ctacgtgcat gcaagtgtgt gtgcgcgcac gcaggagtgt gcatgagacc 88140 agtagagggg tctttttgtc cattgtcatc actgtcttca aatcagccat atcaggcact 88200 tggaacttgt ccctttcata tacttcctta gaaatccctc cgttcaatgt cacttttctc 88260 atttggactg gttaaacaat tccctggact gttctctaga aagacagatg tgtgaataat 88320 taactcaacc tcattaatca gttgctaccc cggtttctaa gcttcaaagc tgagttactt 88380 gcccttagag catagaattg gtttataaat ggtgtatgca ttcatgtatg ctaaaggaaa 88440 ttatacgttt tatagaactg agaatatgta aagtatataa aatgtactgt gaaaaaaatt 88500 aagaagatag agacgacttt ttaagcaaat tcaaagagct taaagtctta ttgtagatag 88560 tgttttaaat ttttttcact tataattatt gtgtttgtgt gtgactgtgt gtgtgtgcat 88620 gtgtgtatct gttcatgtgc atgttccaca gtgtgcatat ggaggtcaga gggcaaactt 88680 tcagagttca cgttccactg tgtactcaga ggctcaaatt caggtagtca ggcgtgcaca 88740 gcaagcatct ttctacaccg agggatcttg caagcccaag tgacatcata tcgatactct 88800 taaaagtcat tgccttcaag ggcaaaggtg atgtcataaa aaagctcgtg cttaagtcag 88860 cttaagtcag agagcagcct gcagagttct gtgctgctga caccgtgctc attgcactaa 88920 gcctggaact atgggaagct cacagataac caatacacag acagttccct gaagactgta 88980 gtcaaggaga cgggtgcaca cagctaaaca gaccacaagc cagcgaagca gatgctgtga 89040 cagtcactta aaggacatgc tgtgggtgag actagggagc tgggagtagg tgccaataca 89100 gcaaaattcc ttgtgcttac caaatggtcc atcctcactg tcagatggca ggggcatgca 89160 ggacacagtg gtggtctttg acttggttat gaaggatgaa aagacttcaa caggaagaca 89220 aattggggat gaagatgtag gcagtggagg gaaaggagaa gcagtctaac tggggctgaa 89280 gagataacca gaggacacag tgttggatat tttcactgag attaatcaac agttcagtct 89340 ttggtccatc agcccctcct ccacacacac atcccccact cgtttttttg aggtatggtt 89400 cacgtgttcc aggctagcct caaactcacc aagtggttag ctgaagatga ccttgaactt 89460 tagactctcc tgcctgtact tccccagggc tgggatgaca agtgtgcact gccctgcctc 89520 aggtgtgcac tgccacgcct ggcttgtgct gttactagag atggaaccca aggcttctgg 89580 tgttctgggc aagcacagcc tggctcacat ccccagccct ggtccactct tgtctctttc 89640 cacagtgatc tatctagtct tggtcccagc accttgctca gagggagtgg tcaccgatga 89700 cctctccgct gtttacctgg atggaccctc cttagtccct cattccttgg cctcttgctg 89760 atgtcacttg tcacgtcaac cttgatgtcg atggccacac tgacatttgg gtcccttgtg 89820 gtcttcatct gtaacatcct gtccaccgct gtattctctt tgttttgttt tgttttgttt 89880 tgtattttcg agacagggtt tctctgtgta gccctgcctg tcctggaact cactctgtag 89940 accaggctgg cctcgaactc agaaatccgc ctgcctctgc ctcccaagtg ctgggattaa 90000 agacgtgcgc caccactcac ggcaatattc tcttttatct atgttcagat gccttttctc 90060 tgtcagttct tcctcatctt acatctttat gagcatgtct cctgcagtct taaggacatg 90120 ccctctccta caccatgcct gaattgttca tatctaactt tcaggtctca tcttaattat 90180 tactcacctt aggaacagtt tccccaaact ttgcatctgt ccagtaatga gcctagagat 90240 aaaactttat cagagataaa tgcctgagta cccagctgtc ttcctagatt agcccatgcc 90300 attctttagt ttcttttttt gcataacccg attataaatg taaaagtcat gtttaccata 90360 atattcccag cacttaacac aggctaatca atatcaagtt cttaataaat attcaatgag 90420 taaatatgtt attcaggcaa catcaagaat gctgggttga ataagtaggg ttatatagga 90480 agaaacaaga gaagaaatag gctggatgaa gaactttagc tttccacttc aaacatttaa 90540 ggctattcta cagataaaac tgctatttaa agttgtggtc cagtcttcct ctagtaacat 90600 gctttgttag ctggtgtagt gtaaccaatt agccagttta gtcaagggtt agcagggtag 90660 actacaccac aactgtaaga gcagcaagac agaaggactg aacagggatg gtgcccaaga 90720 gaatgctggg agttgatagc gaggcaaggc cccattcggc atccttttct gccctgttat 90780 cattcactag gagaaaagag tgcctaagga aagccagact tagaaggaag agaggcagag 90840 agctcatttc ctgatgcaga atcagccggc aggatattag tctatagctc aggggctccc 90900 tgagcaggag ctgtggattt gagagatatt tgggaagaac tattagatga agatgtgagg 90960 gttgtcaagg aaagcagaca aagggaagcg agtcaagagc taggcatgtg tatgaactca 91020 aatagcaaca acaacaacaa cagcaacaaa tgccatgtat acatggaaga gctcgtggcc 91080 gaaatgacaa aggaaacaaa tgttttaaac taaattaaag aaatgatcac gtttagatat 91140 tgtgcttaaa atcttcagga ggtgaaaggt gatctggaag ctgacaccga gcaggggcaa 91200 aaagagagca tgtcacagtt aggtttagtg ggtagaaggt aacatgggga tgatcagaga 91260 accactgaag tgggggaggg gtggtgatat ctttgttctt cagacacaga agagcagaaa 91320 caaggactca ggcatgcgtc gtgacagggt tagaggcttg gttaggtaga aagttaggtt 91380 tccatgtgtc cggggagcgg ggaggagaag aatgtgttca gggcaaagat ccaatgtata 91440 aacttttatg gccaacagaa tgagggctct cagctacaga ggaaacactt aatccgcctg 91500 taaatttcag tccgtcatac taacctgaca tggcagctgg cgtcatcggg acatgagcaa 91560 tggttccaag cagagttgat tgtgcttcct cactctaatt attaaccagt cactgtgtta 91620 gctcattcac gttgagaagc ctcccgcctc actacccatc tgtatggaga cttcttttct 91680 acgaaagaag tatgctcatt ccaaataaca tcaagtgtcc aaatcattag tttttactct 91740 ttgttttcat atgctatggg ttaagtgcag agctattaaa atacaaaaaa aaaaaaaaaa 91800 aaaatctgga atgagccctg acaactgact tgattccaat cttatctgcc attttctggc 91860 tacatcattg ggagacttac ttaacctctc ttaggtcaaa ctacattatt ggatgtttca 91920 gcccattcta attttttttc ttttctcagc cttttcaatg tgtatttact acttgaacgt 91980 ttttcattta aaaaacaaac aaaaaaaatt gaaaaaactc ccagaacatc tgctatgtgt 92040 cagctactga aggaacacaa tgtttaagac ctaggttcct ctcatgagtc ctaagcttgc 92100 tttagtaggc agtgacagaa ggacaaactg gtccatccat ctagataaac ttcggaaagt 92160 gaccgtgtcc atgggaagtc ctccttagac actgtgaccc ttgagttcag cctcagagaa 92220 tggatccaag ttaattgtag gtgtggaagg ttgtgttttg acacagggga caggcaagtg 92280 acaagaaggc actgagttca agggcacagt atattctaag tgtcagttgc cagtggctga 92340 acatagagga gggaaagagg ttagagaagc caagttcaaa gctcagacta tgcatacagt 92400 ataaggttaa ggcactgatc ctatagctat gttacctatg aatgagtggt cgttcttggg 92460 caagggacat aactcaatag gtttttcgct tttccagctg taaagtggaa ttgaaaatgg 92520 tatggcatag cgacctcaca tagaagtatt caaaacaatg tctagggcac ggttatggtt 92580 atggaagcct cttgaagggc tccagcaagt agagatttga gttgcaggca gaggcaagcg 92640 aagagtggat tggagagccg tggaaacggg agtggaaaga tgttagaaaa aaaatacagc 92700 ctgcactcag gagagggtat ggagatacag gtgaagcaga cggctggtgt cagtcttgac 92760 agcccatcag gtgaagatag gatgagcggt aactaatacc cagagttcta cttcgaacac 92820 atagttttct gtttgtaccc taactacatg cacgcgcgcg cacgcatgcg cgcacgcgca 92880 cacacacaca cacacacaca cacacacaca gatacataca cagacagaca gacacacata 92940 aacagacaca cacacacaca cacacacaga gagagagaga gagagagaga gagagagaaa 93000 tgactatatc ataggctttg gggcagggac aggtgggcct aggtacagag tcctaacttt 93060 tatcttgctt tctctctttc tttctttctt tctttcttcc tttctttctc tctctctctc 93120 tctctctctc ccttccttcc ttccttcctt cctccttcct cccttccttt cttctttctt 93180 tctttcttct ttttcttttt tcttttttct ttttttttct tttctttttg ttttttcgtt 93240 tttttggttt ttttgagaca gggtttctct gtgtagccct ggctgtcctg gaactcactc 93300 tgtagaccag gctggcctta aactcagaaa tccgcctgcc tctgcctccc aagtgttggg 93360 attaaagacg tgcgccacca ctgcctggtc tacttttatt gattttttga tattttcctt 93420 ccctcacaaa gaagttgaca aacagcctgg cagagctgtg agaactgaat ggagactgag 93480 taggaaccgt gccctggatg gtgcttggct ctcatacctc agatcctcag ggaaatgggc 93540 tggactgggg cctagagcac tgaatcccct ggaaggcccc tgttgacaaa atctctcccc 93600 aaacccgaca aaagcatcac aaactgtgtt cttcccagca cctacaggag ctacacctta 93660 gaacacggat gtttcccagg tgttctttta ctgtcctatt tctaggaacc cgggactcgc 93720 acttgtttga actctagggc gtttaagaag tcactgataa ggcagagcca gatggaggtg 93780 ggatgagcaa gaactgctac tctgaagaga gggacaggtg cacagaagac taatggctca 93840 gtagacagaa aacccacctc tatctacaga taagccaggt gacaacatta gcctcctcgg 93900 cagctgtccc ctcttctgct gagacctatc gattgacatc ctaaatgtca ctgtgaaatg 93960 ttcaagggat actccccact tagcgtagct gctgagccag ggaaggcaac tgaatgtctt 94020 atttgaatgg gttgcctggt gctctccaga aaggttccag gctgggacgg aaccttccca 94080 agtgaagaaa ctcacttcac aagcctccag tccccgccct gcaacaagcc tgtttgttaa 94140 cttgaacttg cggtgacaca caccgagttc atttccagtt tgcgctccag ggcaagtcct 94200 tgactacaca aagagtgaga cagttgggga atttgtggac tgtccagtta atcagataaa 94260 acccgagaga gtcagaaagg cgacttcttg gaagatgtca aagttttact gaaaacagcg 94320 gaagtgttcg cagctaattg gtgctttcct tccacacacc ccgcactccc taccgccccc 94380 tccctccccg cctcccatcc ctgtccagtc cccaccccct gaagtttgtc tttggtaaac 94440 cccagctact tctctcactt gtctctgagg cacaatggga gtcttcatct gaattctagg 94500 gaagaaaaga tatgactcct tctagcacga gtgagctgga ttgtaacagg gaacgaattg 94560 attgattttt cccccccttc atctcaatga acacactatg tagccaaacc aaggctgatc 94620 aacaacactc aataaaaaag gagattcaaa ctgaggccca gctgagccct actagacctc 94680 cacaatgcac gctgtcatct gcttcttaca tgtcatcaca tttaattata ttaatcttcg 94740 ccataagtaa tggaggggat tattatgagc cttggtctag caactgggtt caaggtcact 94800 tagctaatat gtaactctaa atgttacaag tttctaagcc aaggatatgt ccttttctca 94860 ggccgatgct gtcctgctgg ccttctaaaa tgttagttct ctgcctcttg ttctgtgcct 94920 acctcagtgc cccaccccca ctcctgctcc cccccccccc attattctga tcacatttac 94980 attcccggtc tcccattctc ctgatcctgt gtgcttacat cagctagaaa ttatatgcag 95040 gacttttaaa gagttttttt catagtattg cctttttgca tatatatata gtgttctttg 95100 tttgtttgtt tgtttgtttg ttttcaaaac agggtttctc tgtataatag ctttgactgt 95160 cctggaactt actacagacc agtctggcct tgaactcaca gacatcctcc tgcctctgcc 95220 tgacctgtca agtcctggga ttaaagcttg caccaccaca cccagctgct tttatagttt 95280 tttttttttt taagttgtgt ggtgtgcatg ttgttgtgtt ttataaaata gctgtggtat 95340 aatgtttggg gatggggaac atttcagcag gcctctgcca aggcataccc cttgaggaat 95400 cagtcatacc acaggcttag tataaaaggg ataggggacc tggaaaaggg gcagaggcag 95460 agaaggtggg ggacagagga ggacagaaag agagagaggc cagctgggaa catgttgggg 95520 agagatagag agagggagga agggaaggag aaaagagaag agagagaggg ggagggagag 95580 ggaggaggag atgaagagga agggagggag gagagagaga gagagagaga gagagagaga 95640 gagagagaga gagagagaga gagggaggga gggcaagcag tcctttttat acaccaggtt 95700 ggctcacact tggcaagcct caagctgttg ctaggaagct gttggatgga gcctaaagga 95760 actgccaata gtgtgtgtac agatgagttt gtgtgtgcga gtctatgtgt aagcatgcag 95820 aggccaaaga tatcgttcta tatgacaggg cctctcactg agcttgaagc tagtcatttt 95880 ccccaggctg gctggccagc aagcttttgg aatctgcctg tctctgtcct ccaaagatgt 95940 ggttacaggg atacatggta gtgcctggca atgcctggct ttttatgagg atttgaagtc 96000 aggtccccct gcttgcatag taaatgctct tacccactga gccatcacct cagactcatc 96060 ttattaattg tttcagtgtc gctgtatcgt ggacacaaac atatgcatgt atataatagg 96120 gggagagcag attattctgg catttcgaga tcctagccca gtgcaggtgg gtggcagtat 96180 tagcaaaatc actctgatct gatatctgtt tattcatgta ttcttatcta agtgttggaa 96240 gaagaaatgg cacgagaaag cacagtaact tagttcacag tcacaaagct tggcaactcc 96300 tagctgggtg gtgacacggg ctgtacccct atcattcagg aggctcaagc agaaggctcc 96360 cgagatggga gctagcctgg gcgttggagt aaatcccagg gtaactttgg cttgtgtagt 96420 gagttcttgt tttgagagac tcacgtctgg ggagccagag cagtagctca gtggttaaat 96480 gtactttctg tgaaatcata aagatgggag ttcaaatccc agaacctgtg tactgacata 96540 ctggggaaaa cctataaggc cagctctgga gggaggtgga ggcaggagga ttgctggagt 96600 tagttggtgt cttagtcagg gtttctattc ctgcacaaac atcatgacca agaagcaagt 96660 tagggaggaa agggtttatt cggcttacac ttccatactg ctgttcatca ccaaggaagt 96720 caggactgga actcaagcag gtcaggaagc aggagctgat gcagaggcca tggaggaatg 96780 ttctttactg gcttgcctca cctggcttgc tcagcctgct ttcttataga accaagacta 96840 ccagccctga gatggtccca cccacaaggg gcctttcccc cttgatcact aattgagaaa 96900 atgccttaca gttggatctc atggaggcat ttcctcaact gaagctcctt tctctgtgat 96960 aactccagct gtgtcaagtt gacacaaaac tagccagtac agttggcttc cagcgcagac 97020 aggaaaattt gaatcttgat tcagaatgag atccgtcctc aaatgaaaag ccagaaagta 97080 acagaggagg acactcaatg ccctcagtgg gcacgcgcac aggtttgtgt atgtgcacag 97140 aagatggtat actattacca tacttgtttg tttgtttgtt tgtttgtttg tttgagacat 97200 ggcttctctg tatagctctg ggtatgctgg accaggctga cctcaaactt acagagatct 97260 gcctgcctct gcttccagag tgttggggtt aaaggagtat gccaccaccc cctgtggaca 97320 cagatgtatt cacaaatttt caaattgcat ctgtttgttt ggctagtgca cctggcacag 97380 agcgtcctcc acatggcatg gatgaagact tattccaatt ggttattgat caaactaatt 97440 catgaaaaag acagctgtct ttcacctgcc cagacaagga gcaaaaattc atccttatct 97500 tatggattgt gcaccttgca caatccatac aaatattttt tataggaata aagatttggt 97560 tgtaagattc atatattaca gaatgaaaca gggagataca gctctgacaa gattcatcct 97620 tggggatgct gagatgaccc gctgtcccag ctcgctgcca catcctgcct cacctgatgc 97680 tgtttccaga gacctgcttc cagagcagcg tcaggggtgg ctgctgattc ccatgacatc 97740 agttcatcca gcctttcaga cagatccacc aagacttcag ttaagccctg aacattctaa 97800 gcatacaggg actggataac aaatgcgaaa actagctttc ttaactctaa ccaagttttc 97860 ttatttccct acacctccca cccccttgaa aaaaattcat caccccaatt cagcaggaag 97920 tagttatgga gagttgtcat cttgattcct tgggtttagg tgtctggcta tggttatttt 97980 aacattatta tagatatttt aattatttaa ttattattaa attatagata ttttaacata 98040 ttaagggata ctttggaaat gatcataatt ttagacaggg aggaaactag agaggttaga 98100 cttggagatt ctgtcttttt ttttttcttt cctttgctct agccttactt tcttaggtaa 98160 aaggaaggga gctgaaatga aaatggggac tagagtaata taggaaatta gagatgtgag 98220 atggattatt caatttactc ttaaacaaaa cattttatag ttataatctt tcattggtag 98280 aaatctttac attttgatgc agaactaaag ttatattttc ttacattggt acagaattta 98340 tatatcgata caggtttaag gttttcactg gtataaatct ttgtatattg atataaaatt 98400 taaaattaat tctgttactc ttagacaggc attgtgccta tacaactcat ttatacaata 98460 caaggcttag acccagtcct tctaataggt actgtcacaa atggtttaag atgattaagt 98520 gatgcaagtt aatggtcaga tagtaaactc atgctcatat tagattctga tttaattaca 98580 ataaagtgtt ttcaagatta tcacgtagga aataactaag agtaatcaat gtttaacaat 98640 tcaattatat tacatataga tagatggtct tcaaaaacat cagaggtcca caaaatatgg 98700 catttaaaat catttcacta ttaataggtc ttttgactag gagacatgtc tgctcctaac 98760 agtaccccca tctcactgca aagaaggtac tgagtatcaa tacctcccaa ttggagttgc 98820 tttgattgcg atgagctagc tgctggtaag aaatagccta atttaattct acagacaatt 98880 actgtccaga aaggacaaat ggatgcagga cagttgactg ctaagctctg ccaaaacaga 98940 gtaagctagt cctttatatt tcctgcttca tttatggtct atcagatcgt tctggaccag 99000 aagtctgaag acaggtgctc caacattata aggaatattg aggactgtcc aggcagtcag 99060 ttgttcctac aaattgtatt aagttttaga agctatgtct ttgtgctttc tacatattct 99120 gataatttta aattccttct cagatctctg atggaattga aaaatagtta tagtctcata 99180 attgaactta agttatttag cactaaagaa gatgttctag atttgaaagg atggttttct 99240 gagggtattg caagttaaaa tcaagcataa ttgaggtaca gatttgtaaa ttctgtaagt 99300 taagatagat aatagagtac tttatctaaa ttgtcagata tgatgtactg gatgttctaa 99360 gtgtatatca taccttacaa tttacatact tgttataact gtgttcaatg tatatttggg 99420 agaaaagagt ttttaattgg acaaaagggg ggaaatgtag ctgtctaatg ctaattatgt 99480 ttcctcataa ctatttgcat gagagggtct gcacatagct tctgggaacc ttcccacagt 99540 taattctgat tggtaaataa agttcccagc agccaatctc tggggagaag aaaaagaggt 99600 gggtggggct taggtttccc aggtgtggga cctctgggaa gagagaagga agagagactc 99660 aatgcctgaa aggtagtata ggacaaagag gcagagccac cctgtaaagg ggccaggaga 99720 gagtggccca gatggctgct caattgggcc aggagaggcc aagatggaac atagaaattg 99780 gtaagtagta acttggaatt attggcggga ggtagagtct agcagtatgg aggctaggca 99840 gatgcccagc tattgtgctg cttaagtcat attaaaatat aaaggctgtg tgtgagtttt 99900 tcattagaga tcataaacca ttgaggtagg aagtaatgct gtgctgggat ttattaaaac 99960 attaatacta cacttgtggc aatcacagga cagacaactt gtaggaatgg attttctcct 100020 tccaccatgt gggtctaggg aattgaattt aggtagttta cagccttggt ggcaagcatg 100080 ttcaagccag ccccaattta atgattttaa ttaaagttgt tgtcctctga ggcacaaaaa 100140 aaccccaaag ggttcaacac aatcaggatt cctgctggcc tctcatctac agcttgggcc 100200 agaagccttg ctctgttttg catttggaat attttatctc agtctctgta agtcaggccc 100260 agggatgggt atagtcagta cacatgaatt tggtctgcat aactggaaat gtgattatag 100320 cttctcagct gttccaaagg agaagcacca tcatatccca ttctgccttt gtgtgaaaaa 100380 ctcctcaaaa atacatatta gtttgtatct catatatatg tatacatata cacatgtatg 100440 tatgtatata tgcatgtatg tatgtatata tgcatgtatg tatgtatata tgcatgtata 100500 tatgtatata tgatgtatat ataatgtata tatgatgtat atatatcaca gacacatata 100560 atttaggtca ctttttgtcc ctatgtttta tttctttcaa aacctactgc tgcgccttac 100620 acagtgtttt gagaccatct tctttcttat cttttttttc tcacataata tatcctaatt 100680 acaatctcca ctccttctag tcctgctagt tcctcccctc ctatccagag ccacctttct 100740 gtctctaatt agaaaacaag cttctattat atagattata ataagacaaa acaacaccta 100800 acacaactga ataggacaaa caaaaaggca gaaagaaaag agaagtcaca agaaatagat 100860 ccacctgttc acacactgag gaattccata taaactctaa gctggaaacc ataatataat 100920 gtgcgaagga cctttagggt aaagagagag aataaaactg cataaataaa ataaagtaaa 100980 aataataata aaggaaaatc cctgacacag cacagtaaga cagggaagct ccaaagactc 101040 tgttgagttc attctctgct gaccatctag ttctggacat gtaacctacc cttaagagtc 101100 gtttgtttcc acagcaagac tgtcttagag gaaactaaat tttcatttgg aaatggttat 101160 caactggaat tgcttctggg ttaggcctag gggcttgtgt ctacatcttc tctcagctct 101220 agggtccaat cttgtccaga cctgtgcagg tccatgcatg ctgcctcagt ctctgagttc 101280 atatgtgtat ctatcggccc ttgttttctt ggtgtgcccc atcccttctt gctcttgtat 101340 tttgcctcct tttccctgag ccccaaagga agggatctga tggagatagc ccatttaggg 101400 cagagtgctt taaggtctct catttcttac aaatatctgt ctgtgagtct ctgtgttccc 101460 atctgctaag ggaggaagct tctctgatga tggctgacaa gacgccaatc aatgagcatg 101520 tcatcagggg tcattttatc gttacatttt tgtttgctta aaaatagtaa tatttggttc 101580 taccctaggt tcctgaacta gcttgtctca ggttcttggt tacccaagca gtgtcaggtt 101640 tcatctaatg gagaggacct taaatcaaat cagatattag ttggttacta ccataagatt 101700 tgtgacacca ttgccctagt gtattttgca ggcagggcat cattgtagat caaagagttt 101760 gtggctgggt tggggtttac ctctctcctt tggtagtgtg ccttcccata ccaagaactc 101820 tggtagataa aggtgaaggc tctatgtagg caccagctca atttctccac gttcaatggg 101880 ttgtgtaggt gttgtcttca gcaattttta gggtcactta tatatactat catatcatct 101940 gcaaataaac acactttttc ttctttgttt ccaatttgta tcccattgat tttctttaga 102000 tgtcttattg ctctagctag aacttcaagt gctatattga aaaggtatgg agagagtaga 102060 caaccttgtt ttgttactgc tttttagtgg aattgctttg ggtttctttc catttaagtt 102120 gatgtggtct tgctgtaagc tacctttatt atgttgattt atccctaatt attccaggac 102180 ttttattatg aaggagtgtt gggttttaac aaatgccttt tctgcttcta atgagatgac 102240 atgtggtttt gtctttcagt ctgttaatac gatggattac atttactgat tttcatatcc 102300 ctgcatctct gagatggtgc ctacttgatc acagtgtatt atctttttga tgtgctcttg 102360 gattcatttg caagtatttt attgacaatt tttgcatcta tgttctgttt gaatttggtc 102420 tgtagttttc tttccttgtt gagtctttat gtggtttgag tatcagggtc actgtgacct 102480 cataaaacaa attgggcagt gtcccttcta tttctacttt gtagaataat ttgaggaata 102540 ttggcattaa ctcttctttg aaagtctggt agaattcttc actaaaacca tctggccctg 102600 ggatattttt tttttttttt tttttttttt tggttaggag acttttaatg actgcttcta 102660 tttcactagg ggctataggt ctctttaaat tacttagctg gtcttgattc aactatcata 102720 agtggtatgt atagagaaat ctatgtgagc tgaagagatg gctcagcagt taagagtttg 102780 ctgttcctct gtaagacctg aatttggttc ttagaaccca gattgggtaa ctgatagctg 102840 tctgcaactg cagttccaag agatataata ctctctcttc tagacactgg acatggcaca 102900 cacacacaca cacaaagttg aatgcaaatt aaatggtaga taacggtatg tgcacttcag 102960 agtttgtggt aagtgacagt tgatttactt gatgcaacac agagtcgtct ggaaagcaaa 103020 tcttgtgcaa ggatcgccta ggttaggctg gctatgggca aggctgtggg gaattgtatt 103080 agttggttac ttgtggtaag acctatcctg attatgggtg gcaccacctc atagtctcag 103140 tcctgggctg actgaaaagg agtaagctga gccctggcat gtaagtatta attcttggct 103200 ctctgctctt gactatggat atgatctgac tatctgtgtt aagttcctgc tgtccccact 103260 gcccaggagt gatggcccta gcctgcaact gtgagccaca gaaatccctt ttctcttaag 103320 ttgcttttat ttcggggttg ggtggtgggc ggtgagaccg aggttttgtt tttgtttttg 103380 tttttgtttt tgttttgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 103440 gtgtgtccgt gtgtgtgtcc tggctttcct agaactcatt ctgtagacca ggctgacctc 103500 aaattcagag atgggttcct ttgaggcaag caacttctga aggctaggaa ggctcaagca 103560 cattggctcc tgggtccaca gtgctcagca tctgtatttc atgaacagtt cctgggtcct 103620 ctgccagggc tgtaagaagc acggtgtctg cttaatacaa ctgcagccaa agaagacaga 103680 ggtgttggtg caaaaacaca gctctcacat caaagcaggt aatagtttat tctgaagcca 103740 aatatgagtc ccaatggcct agtaatacag atttaagtta cccagcattc tgtgtccaat 103800 gtgctaagat ttggtaaagt ttttatggaa gcagaagaag tttattacaa atccgggtac 103860 ttttcaaaca cattggtgga ggcatcgggt aggtggatca cagcaaagca ggaacaactt 103920 tacttgaggc tcagatgctg acagtgtttc acagcccatt ggttggccgg aaactaagag 103980 ttcgcacatt ctacggaatt tacctaatgg cacatggaag ggcaataaat ggctgtttag 104040 gatcctaaag atgccccaag ataatttggc tctagacttg caacattcta acccctctac 104100 atcattgaca ttctaatcag ttccgtcagc ctttccaggg ctggggggta ggggaggaag 104160 gcaaggctat ttgaggaata gcttcactac ttgaacacca cacattcagg gctgcaaagg 104220 acacacaagg ctctggtctg tggttactag acctctcatc aattcatgag gttgtagcta 104280 tttcgaactc tggaccatct tgtagaggaa tgtgaatgag gctgaatttc cgacatgcct 104340 ttggacagat gttcgtgatg ctaactctag tttttgtaag atagttattc tcttctgtga 104400 agtgatgaag aatttgacac agttttaaag aatctccgtc tctctgactc cgtctctgtc 104460 tcaatctctc tctctctctc ttcaaattag aggtttggta atttaggcac agggctgcta 104520 tatgactctt tgatagcctc acaggcatgt gtacttagtg tgatgctcag cactttgaag 104580 gaaggaagga gggaggaagg gagggaggga ggagagagaa aggtgaaagg agggatgaag 104640 gagggaggaa agaggaaagg aggaagcaag gaactcagtt ataggccaaa gagttttcag 104700 gtttatagac acatagatgg ttgcagctgt agcctggcag tctacataaa ggacacataa 104760 agacattctc ttggccacag gttgctaagg ctgggtagga agtctgtaga aggaagccag 104820 aagtcagtaa gtcacagatt aaacattgag caaaggacca catcattagt cactcaatgc 104880 tagtgtttat ttgtctttct cttttgtttg tataaatgga agtactctct agggtttctg 104940 tttaattaaa aatcttatta aatataagca atgaaactgg cttaaccagg aaactgaaca 105000 ttaaacccgt tcacaaaacg tatgatctga ctaggagaaa gagaaatgtg ctacccacac 105060 caatcctggt tggacatgtg cctaagccga ggtcactact agattcagtc agtacccaga 105120 gaaggtagca aacagccttt gccatataac tgaatgaagg gccagcctca tatctgggtt 105180 tctaactgca gaacacatac atgggtgtgt atcccaggtc aggttgtggg tggcaaggcc 105240 ttgctgctgt ataggggtca gggctcttct tgcatatctg tgtttataac aagagttgct 105300 tctgggtggc acagactagg ggagacagct cctccttcta gtatctttgt caagggaaca 105360 cactataacc ttccatggtg gttagcttaa ctatttcctg tgggtgtgtc ccttattaaa 105420 actatatcct aaatatggtc ctctcttagg aggcatccca tttcctagtc agtcatcata 105480 catgatatac aaagagaggt atcagggcct gggctccatg gctgtctaga tgtttctcat 105540 ctgttgtggg ggatggtgca gcatgccctg agctgcctga gtcactgcag gaatgaggaa 105600 gactcacagg gaggagggac caatttcctt cttcagtaga gtcagagagc tgcataggtg 105660 agatctgagc tgtgcaagga gtgggcaggg cttagatgta gaaaattttt aatcctgacc 105720 tggggcttct accctgcctt tgactcttta gttcccagat agaagacaca cacagccttt 105780 ataattacaa taaaccttca acagcacaag agctgggtgg atacttaccc tctacgttat 105840 tagaatctac tttcctattg ataactctga attattactt actacttaac tatgtttcat 105900 ctgggctgct cttaactcca attggccagc cctcagggcc atgtttttat gattcccaaa 105960 ccatggcacc ttctcctttg tcttctgtac cttctagttc tccttggttc tcctctgacc 106020 catgaggtca ccccaaacct ctaatccctc cagtaattgg ctgtagagaa ttttatttaa 106080 ccagtagctt taaatcaagg gacaaggttt gcagaacaca agcttgtaaa caggagaagt 106140 cttttgtagg ccaagacctt tggctataga atttagcatt acaatgcata gcatcagacc 106200 aaacctcaac acttagaaac tcagcaaggg acaatgtggg caaagacgtg gagtgagagc 106260 cccctcaggg accccatggt cacctgttcc ccattaacca caggtctttg ggattctgca 106320 aacccccagc aacccccagc agctgctgga agcaccatcc catactccat gagtgtgtat 106380 ccaagctttg caattctgaa gtcctatgag acaatgtgag tagtgtgtca ttgtcaggat 106440 tcatgtatat tgtagacaca agatttaatg aaatctttct attgtcaaaa gtgtttctaa 106500 aatgtttgga attacattag agcagaaaga gctccaattt atgttttgct aggtaccttc 106560 ccaacttagt agctttattt atttatttat ttatttattt attatttttg atcatgtgca 106620 ggctcccaaa gtgcatgtgg aagatcaaag gacagatttg tggagtcatt tctcatattc 106680 taccttatgt gggctccaga gctcatactt aagttgttag gaatgtgagg aaagtgctct 106740 acccattgat ctatcctgct gaccctttag ccattttaaa tagcaaccat ttctgacagc 106800 atttggcatc tgaagatcaa gaattcaggg gctccttagc tgtgtgggtc tggccccagg 106860 ctctcaaaag gcagtgagca catcttggcc aaggttatgt cacttgctgt tgaaagatca 106920 aattctttgg gttcagactc catcttagac aaacttgact taagtaaatt aacaggtcgg 106980 tacctagctc cagtttccag attacccaaa acaaaacctg agcttagctc cagtttccag 107040 gctaccccta acaagatctg gatctccagt ttattctcct ctaacaaatc cactgtctag 107100 caccaatttc caggttaatc cccagcaaat cccaacacct ccaggttaca aggccgcccc 107160 tgcctaacaa ccaccaatta ggaggaaagc caacgtttgg ctcccagcac cagccaatta 107220 caccaaaggt cacagtagca ccccaattag atgcttgcta ggcttgaaac actcaccctt 107280 cacttgtgat ttcctataaa accttgtccc agtgagtgat caaggaattt ttccatggaa 107340 ccgtcctgtg ggtcagcagt gaatcaagac ccacgctcga gcttgtgaat aaagtccctg 107400 gtgtgatagc atcggatccg gctccttggt ggtcttttgg agttcatgaa catttcagca 107460 tgatactgtt taactggcat gggcattctt cttgtggtgg gcatctgttc ttcaaatggc 107520 gcatctctgt gtgagtgagt gtctctatgg catggtggtt ggtcgccact gaaatgactt 107580 ttgtagaaga gcaaacagat aaaaggttct tttgtgccgt agtctctgtc aaaccatcac 107640 ttctgctgta ttttatcggc caccctgagc atccttgatt tgatgcagaa gaggattatg 107700 caagcaaata aataccagag gatgtgtacc actgggaaac aaggcaaggt cacctctgaa 107760 ttcatattct gggaagatta gagctttcag aacaggagct gggcacatgc ctgtaagctc 107820 cacactcaga aggccgagca gatggttcac accagtctca gttacacaag tcagttaagg 107880 tcaacctggg ctacttgaca ccctgtccct acaacaacca aatatacacg catgccagag 107940 atgatatagt gaactagaca ccctaaaaaa atttcacctt taacgtattg aaaaatactg 108000 agtagaacat tttaaagatt tattttggtt tttaaatgtt atgtctgtgt atatgtgtct 108060 gagtgtatat cgcatgagtg tatatcgcat gtgtctgagt gtatatcgca tgcatgaaat 108120 acccacagag gacagacaat gtcgccagaa ctctaaaact ggcgttacag acacctgtga 108180 tccaccacgt gggtgctggg agcccaatcc tgatcctctg caggagcaac acaagctctt 108240 agtcactgag ccatctctct agccctaaga gtcaacaaat aaacacacac acacacacac 108300 acacacacac acacacacac ttaaatcttt aaaagctgcc ccattatact atcaggagaa 108360 taacaggtaa aaccaacaca ggaggaggtg tggattgaga gactcgcgga gttgcaggct 108420 ctccttctcc tgctctagtt cccagtagct tactattcat ttaacaatgt ttatggccgt 108480 ttgctaggcc tattcctcat ctaactccta acttacttaa cctgtttaaa ctattctatg 108540 tctgccacat ggctggttac ttctcagtat catatatcta atttcctcag agtgcaggtg 108600 gtgaacctcc cagagtttca gtctgtctac tggaactccc agtttctact tcctgccttt 108660 ggcaaaaaac accatgcggc atatttggtt ttaccccttg acctagaaaa cctcaagtgt 108720 ttatttcctg gccctttatt ttctaaaatg ccaataagat aggccaccag atctttaatg 108780 acaggtgatg cttcgacaca gtacctgaga gattatctct acagacataa gtgcttaagc 108840 tgggggctcc tctaggcaca tccctcaacc accaagtgac agggaagctt ggtgagtttc 108900 tttcacatgg caacaaacaa ggcacctcag ctagcaagca ggaaagcagg agataacatg 108960 tgacggatgt aggcgtcaga gcaaagaccc ttccagaaac tgcctcaaat ctcaatggat 109020 atttctcagt gatagttcac atcaggcttt tttttttttt atgagtacta tttttcttgg 109080 tttattcaat aggctgcttc aatttacctt aatgtccact tatacttata tataagcttc 109140 ttttatacaa gctcagtgga ctcactgaga aagcacaact aggaatgcaa atcagacagc 109200 aatattaata cagagttcac cttctccttt caatggaata aataatgcgt gcatttgtct 109260 ctttctaaat atttaggcac caaaagtttt catgggactc tttctttttt attttattta 109320 cttattaggt attttcttca tttacatttc aaatgctatc cccaaagtcc cccataccct 109380 cccccctccc actcccctat ctacccactc ccacttcttg gccctggcgt tcccctatat 109440 tggggcatat aaagtttgca agaccaaggg gcctctcttc ccaatgatgg ccgactaggc 109500 catcttctgc tacaaatgca gctagagaca cgagctctgg gggtactggt tagttcatat 109560 tgttgttcta cacatcaggc atttcttaat tctctttcca catgcaagga gataaaacct 109620 ttcctacttc tacagtagct ctgatggtca gggaaagtca ggaattataa gcagagtctg 109680 ggtttatatg ggcttgcagc cacgctcacc cagcatgagt tacagaaacc tggctgatag 109740 tctgtttgga aatgtgaccc agcatgagaa gtgttctctg tctagaaaca aacgggagtc 109800 ctgttgaagc acatgatgtc tttggttttg ccccttcgtt tagaaagcct aaaaagatta 109860 ttatctggcc ctttatttat ttttaaataa atgtcaatgt tagttgcaga aatacctgca 109920 tagaagcccc cagttagcct agagttgaaa tacaggaaag atgcatttat caaactaccc 109980 tgtgcagcag aacaaactga attagagacc ggggcaggag gaagcttggt tagacacagt 110040 cttaggcttg gttaaaaaaa gggggggggg gcgttatgca ttatgtacag ttaactgggt 110100 acctgaaata gaccagactt gcttctgagc actgagggct taatggttaa ctataaggag 110160 cctagggagg aagattggtt cgagaacata gttactcctc aattgtgaat ggagttctgc 110220 aaacagagaa cattaagagg aacagaaaag gagaattctt ttggaataat agctctctgc 110280 cacatgtacc caggtgctct gatccccaaa tacatcaaag ctgctttgtc acccagtctg 110340 tgccagaatg ggcttgcaca atgcatctga gcaccgagtg ctaatatgct ctcctgagag 110400 cagcagtggg gagagtctga gctggtggca gtgagctagc tgcagattgc tctgggctgg 110460 tggcagcagg acaagtcaat taggggagtg attgggagaa aggaaggcaa gaggcaccca 110520 gagcctccag gctcttgttt gaaaaatgga agacacacct ggctttttcc acatttccct 110580 aggtgctcgc tagggcctgg tcacctgctc tcctgattga ctcagcaggg attgggttgt 110640 aaaattaggg gctgggtgat gaggcagcaa cttccgagtg cctattctgt gcatgtcttt 110700 gtacatttat caaggccctc aaatcaccct cacagagcag atattggccc cttgttttgc 110760 aaatagggat accgaggtcc agagagaggt actaagacta caaagggaga tgccaccccc 110820 aaatccttct ttctttatct ccaagtaatt ctagccaagg accacatggg agggtgggtg 110880 ggctcagccc agctctcctt ctaagaacaa aaaggactta tgatgtcccc tgggctcccc 110940 atttctctgt gagagttttt aggctcaacc catgaggcct agaccttcct gagaatggac 111000 aggcaattta tggtagctga ggaaaggagg aaacattttc tttagctgtg tgactgacca 111060 ctgatgagtg gcacatgtgc ctataaactc actgggtaac atacacagag gggcatgaaa 111120 aaaagagggg ggctagttga gaagaaggga ggggatcagg agtgggaggg agagaagaaa 111180 ggggagcgat gggtgaatat gatcaatata cattgtatac agggatggaa atgtcatgat 111240 aaggccatta ttatatataa ttgataaatg cttttacaaa atcagaacag aaagataagc 111300 tgaggaggaa gttcagaatc acaaataagt aaaataacaa cagggaaaaa tctttctcag 111360 aacgttgcta tggagatagc cacatgctgt gtgagtggag acctcaggtt ttcctattgg 111420 gcaagatgca ttgagtagct acagtcaggg aaagccagga ggtctgaacc tcgatgccag 111480 agagcccatg cagagttgag aacaggagga aggttctgag gagcccggag ggtggatgtg 111540 ggtgacaact tcagaagaag aagggcaggc tggaagccag tatatggaga cagatgtcat 111600 tcctggacct cacggctacc ttgctgagct cactttctgc cagcaggaga aacatgccct 111660 gaagatgtga agatgccaga gaccggtaca gaaaagcgag aggagcgttg ttgtggttgg 111720 ttgtatgtgc tgacttgatc aggctccagg gtgctcaggt atttagaaaa gcatttgtaa 111780 tggctgtgtc tgcaagggtg tcttttgatg tgtgctaccg tgacctggca gagtgaagta 111840 tacattgctc tccccagccc tggtggacct catccaaccc attggaggcc tgtgttgtgc 111900 aaaatcgcag aggtaggaaa atgtcttccc tctgcctatt tttgagcgtg agcgtgaatg 111960 ctcttgcctg tggagtaaga catagactgg accttacctg tgttagacct ccatgcttct 112020 tggattagga tttggactag aatttatcag ctgttctggg tttgctgttg tgacccacag 112080 gtcatcacac tcctcagcct ctgtagcact gtgagaccat tagttataag aactcctgga 112140 gttggagaga tggctcagaa gtgaagaatg cttcctgctc tttcagagaa ctggaggtca 112200 gctcccagca gctgtatcac catcacgtga cttacaaaca cctgtacctc cggttccaag 112260 ggatcaagtg ccctttcttg cctctgtggg cacccagaca tgtgcataaa cgtacacaga 112320 tatacacata aataaagtaa aataaaataa catgaatctt aaaggaagaa acccagtcaa 112380 tcatcaccca tcctgcctgc atcctcttgg ttctcttttt ctctgggtca aatggattgc 112440 cccatacaag catcttcttg ctgccttctc tacaggctgg ggctaaacag ggaagttatg 112500 ggtggggaca actttcagac agatcaagaa ggaggaagga gcgactcatg attttcaaag 112560 acaacacaaa tgttggcagt ttggggatgt aaccgatgag ggcagtggac tcaggggcag 112620 acccgtgcgc atgcgcccac gtgttgccat ggcaccaaca ccatgcgctc aatgggatct 112680 agccacactt acgttaatgc tctggtacta cagagttgga acttttagta agtttgagcg 112740 aggagtaata tatcagtggt gatgagcatg cagggttagg tcccttagag aaatggtgac 112800 tagggtggga agtgacacag ctgttgccat atcaaggcta gagagttgct ccaccgaata 112860 gccataagtc ctagtatcag cctcagagtt caggaactct gcttttcttg tttgtttgtt 112920 tgtttgtttg ttttcttcag acgattctgt gaagggaaga tggaaagtgt taattatata 112980 aactgaagac gtttccctgt gaagtaaggc cagcccatac ccagccttag gctaagtttc 113040 acttgccctt tcgcaacaca aacctttgtc cctagatctg actaagaggt taagcaatgt 113100 gaataccggt ggccaggccc ttcctctccc cagaagagag aagaggtccc cgaggcagca 113160 gatgtgagtc aagtgccttg tggtagagga ggccactgga gtagacctgg gaaaaggttc 113220 tgacactgtt tatgactgtt gggctgggct tgatcagacc aagcaccaaa cgcagagcta 113280 tgtatgcgca gctctgacct tcctccaggg aggatttcag gatgggtgct catgaggctc 113340 cactcctcta tgagtatcgg taggcaggat taatggttgc cccagaaaag taatgttttc 113400 ttcagtggtg tagctactga taagttgccc atgtcattgt aaataacccc tcactcaggc 113460 tcttgtgatt aattcactgg ggcacatgac atcaatgaca tcacacatac acacacacac 113520 acacacacac acacagagag agagagagag agagagagag agagagaata ataaaaaaat 113580 aaaaatttaa aaatcatcta gtctttgtta ataaggataa tatacttggt ggtatactta 113640 aaattggtgg caaaaaatca tgcccgtaag taagcactgt ctgagggctt gcgaaagttt 113700 ctaaacctgt agttacaggc atcctcaaca aaaggcatgg caagtcattg acattagtgt 113760 cacccaggca cacagccaac tcaggtggga attgttggct ctgtaaggac aggcaaagga 113820 aaaggtcaca ctcaaatggc tcatctctgt catccttagt agaaaaaatt gaaaatgata 113880 ttagtcaaga aaggactaaa taagatgata tgcctaatat tcagggagac caggataatg 113940 tttcaaatgc cctggaataa ctggctctgt gtgtgtgtgt gtgtgtgtgt gtgcgtgcac 114000 gcgcgcacgc acatgtgagt gcacagattt atgtctgaat ggtcttcaga aattaggtga 114060 tattccacag gaaggatttt ccatcctatc aagaacctaa gaatatttca aggaaccaga 114120 tcactattta gggaagaggg aaggtcgtgt attatattag ccagatatac ttcactcaga 114180 tatgattttg gattacacat ggtggtgcac acctttaatc ccagtacttg agaagcagag 114240 gcaggacagg agacacccag ggccaacgag acccacatga atgcacaccc cctcatgtgt 114300 cagtgagagc tctcgcacac tctccctctc ataatgagaa atgagaaaag gagaaagata 114360 aaatatctgc tattccttgt attagtcaaa aaacttcctg cgtttgagga tagcttcgtg 114420 tgtacttaga tatttagaaa gtctcaggta aaccagtgct taatagtttg tctcaaacaa 114480 acaaacaagc aaacatgatt tgaaccaatg gcattgggta ggaaggcttt gggcttcaga 114540 taacaaagct gaaggaaggt ctgctctagc cacagctgaa cgtccagtcc tcttctctaa 114600 caggaagtct gcaggaagtt gctctccaag tccacgcagc acttccaggg cgtaagcagc 114660 tcagagtctc tgaacttgcc tgcttgtttc gctgcttcct cttccactcc aggtgggaac 114720 cacggctttg agcgacagag tctcatatgt cagcttcttg aggcaggaga gagagagtga 114780 taatgctaga ggattgtctt cccccttccc cccccccccc gccccactct gacaggaaga 114840 gccttcgcca ccgtgacaac ctgcctgcga tcatcttaac ttagtggata gaccctcaga 114900 cgagtcactg gctgacaaat atagacaccc atgattggct tacagcatag tgttcattgc 114960 ttcctttctt ttccatctgc agtccagtgg ggaccattaa aactaagtca aagggaaaac 115020 tattcatggt tttttaaaat ttttttaaat tttaaagaga gaaatatgaa gggctaggga 115080 gatggctcgg gggtcacgtg tgtgttgtac aagcatgaag attagagtat tatcttcagc 115140 aagaaggtaa aaaacctgga tgtggcaaga cctgtaatct tggcactggg aggtagagac 115200 cgaaggatcc tgaggcttcc tggtcaatca gcatagagct tcagatactg tgagaaaacc 115260 aaatggcaga gagggataaa agaagatccc cggtgctagc ctcatggaca tgcacacaag 115320 catgcaagaa tgcacacgcg cgcgcgcgca cacacacaca cacacacaca cacacacaca 115380 cacaatcata atgagagtag agaaaagtag aaagaaagac agtttattat tccttgtatt 115440 ggtgaaaccc tccactaagg ggcaaaagct attgtacacc atgaacacct gcaggctcaa 115500 cccttgggtg cagagggaga agactgcaca atgaattcca caccaaccat ggagaaacat 115560 aaattaacga acaaatagga aagcaagcaa aaaaaaaaaa actagactgg aaggagggat 115620 tctgggtcat ggaaatatca ttcgccattg atgaatttta tttttatgtt catttttcag 115680 ctccatccct actccttaat gtcgctgcca tgttcctgtg ctatggtaaa tctcttgatt 115740 taaaccaaaa gctttcctta cttcctttca gaatttcact tacgttactc tctctcctct 115800 ccctcactta caatgaataa tcttggaaga aaacatgtct gcatagaaaa ggactttctg 115860 gttagttcat aatgttgttc cacctatagg gttgcagatc catttagctc cttgggtatt 115920 ttctctagct cctccattgg gggccctgtg atccatccaa tagctgactg tgagcatcca 115980 cttctgtgtt tgctaggccc cggcatagtc tcactagaga cagctatatc tgggtcctat 116040 cagcacaatc ttgctagtgt atgcaatggt gtcagcgttc ggaagccgat tatgggatgg 116100 atccctggat acatcactgg aaagagaggc ccattggact tgcaaacttg atatgcccca 116160 ggacagggga acgccagggc caaaaagtgg gaatgggtga gtaggggagt gggggtgagg 116220 gtatggggga cttttgggat agcattggaa atgtaattga agaaaatacg taataaaaaa 116280 atattaaaaa aaaaaaaaga aaaggacttt ctaccttact ggcaactgta ggctcttagg 116340 cgagggctct cgcctcagtc atacgcgtct tcctctctgt aaagtgagct ggctaaatta 116400 gacacatccg aaggtcacat agccccagtt ctgaaacttg atggttctaa agtctgcggg 116460 gacaagcaca tgacccttct ccaacctctg gtatcaggtt ctgttcccac tcgttttatt 116520 cattatctgc tctgtcccct acttaaccca gacttatttg tgcagtctct gaatgagcaa 116580 gccaggcttt ttttttttgg atgatattat tagatgctcc tgaatattct ccaagtgctt 116640 taaatgaaaa ctctggacag tacagccaga cttcccaagt tgccaacttg tcccaggcct 116700 tagaagtcac ctaattacat tgaattctgc ctggtggaga tgaagacaga aagcctgtca 116760 ctaatggtgc atgtgttgag ggtggtgacc actccatctt tattgccagc tatgctaaag 116820 cccttgctgc atcagaagga gtgtgagctt gtaaagttcc gagtctgcag catgatcttc 116880 cattctctaa gcggtaaggc ttaatgtgtg tgtgatgctg accagtgaag gaagattcta 116940 gcaatattct gaaagtgata gataagaaat caatactaaa atccatggct gagaatccgt 117000 ctttggttaa tgggtagcca cagaaggtgg gagagagatt tctggatact tcaaggagca 117060 gaagaataag atcagccaga ggggctgctc tgagcatcga ttgctgctgc tgctgctgcc 117120 cctgccgccg ccgccgccgc ctttgtcgtc tttgtgtatt ttgtggcgct aaaagaatag 117180 caagcaccag gcagcttcta atgaacaaat gcttgtgatt ctggaggcca aatccaaaac 117240 tgagatactg acatctggag gggccttctg gctgtgtcta atatcgtaga cctcatgtgg 117300 cagaaccaca aatggagaga gaaaactaaa agggtccaca ctcactcttc actaggaatc 117360 cactcctgca atggtggtcg taatcccctc accccattct ttcagctgaa tctttttttt 117420 ttttcatcag gttctgccgc ccaacgctat tgcgtaggga gtacttctag cacatgctta 117480 tgggagtgtg ttatcatcac aagagttggt aactttcaag gagacagtgt gagggaaggc 117540 tgtagaaata tttaagaggc aacagacttg gtcagtcaat gaagccagaa ctctaagggg 117600 aagtgtgatg ctcactaaag gaaacataaa ggactccacc cctacccccc acccccgggt 117660 gaacagaact cttctgtgct gtcccagcct tgcacaacag caacactgat accctactcc 117720 cacagtgaca gaaaaggact tgggactcct atgaaggacg agggacttga ctagagctca 117780 ctaccttgac ctttcactgt tagaagtggc ataaggtgaa gagctacagc ttaggggctg 117840 gagtgatggc cacttgctgt tcttccagag gacccaagtt cagttcccag cacccacatc 117900 aggcagctcg caacaattac ccatgattgc taccattgat gctgatgcct tcttccaact 117960 tctgagggta cctgcattct tgttctctct ctctctctca tctgtaaaaa tcatattaag 118020 aaaatatata tttgggttta aaatcaaaat ggaagataca ggttatagag atatatttag 118080 caaaggaacc aagaacactt gatttctgtg ccagagagtc acactttcct caaagagctc 118140 ccaaggagaa acaataaaaa taaatacagc tcttactttg cactagatgc tgttaggagc 118200 actttaactc cattagctga ttttgttctt ataacagatg ccagggcaag agaaagctgt 118260 atcagtcact tgagaattga agttgatttc atggcaggcg gtgggacaga gagaggaaag 118320 tgggctggtc cttacaaagc acagatgcag aaaagttaca ctgggagaca aaaatgagaa 118380 gagatgcaga gtagcctggc tagctggctg ggttcattca tttactcatt ttacctttat 118440 tgagatggtt agagggtaag tctgtgctca ccatagtgac aaacttttaa atgatgcttg 118500 tgcttcagcc ccctccagta tcacctcagt cttatcattc gaacacttgg tcctgacggg 118560 ctttgctttt tgaccttgta cattttttta aaaagcgatt tttatttctt tttatattat 118620 gcctgtgtga tgtaattgtc ccacatgagt gccacggcca cgggagcctg aagagagtgt 118680 ggcatcttct ggaactagag ttacagattt gtgagccctg tgtgggtgct agaaaccaaa 118740 tccaggtctt ctgcaagagc agcaagcact cgtagctgat gagctatctc tgtagcttcc 118800 ggataattat tttgatgaca aatgtctacc tgagcctcca tttataacat tgcaatcatt 118860 cctagagatg tgcactagta gatttgtaca atagagtgca aatcagcata tttcatgaag 118920 acttaaaagc ttaaggtcct tcagccattt ggaatgggca aactgctggg acatttatac 118980 catgcagtgt tgtatagcag agaaaatggg tgaatcatca ttacatgtga gtgaagggct 119040 ggagagatgg ttcagtggtt aagaacatgt actgctctta caggagctta gtacccagta 119100 ccatgtcagg cagctcacaa ctgcctgcag ttctagctcc aggcgggaat cgaatacctc 119160 tggcctctga gagcaggcat ataccaccat acacatatat gcataattta aaatgaaaat 119220 aagttttgaa aacaattaaa aaataacagg atgaataaag actgaacaac atgtttgagt 119280 ctatgccggg gcctagcaaa cacagaagtg gatgctcaca gtcagctatt ggatggatca 119340 cagggccccc aatggaggag ctagagaaag tacccaagga gctaaaggga gctgcaaccc 119400 tataggtgga acaacaatat gaactaacca gtaacccgga gctcttgtct ctagctgcat 119460 atgtatcaaa agatggccta gtcagccatc agtggaaaga gaggcccatt ggacttgcaa 119520 actttatatg ccccagtaca ggggaatgcc aggaccaaaa agggggagtg ggtgggtagg 119580 ggagtggggg ggtgtgtatg gggacttttg ggatagcatt ggaaatgtaa atgaggaaaa 119640 tacctaataa aaatattaaa aaagaaaaaa aaaagactga acaacaaaaa caaaccagaa 119700 gttccaggag aatattggct ccgcacttta tcttttttaa ggtccaaatg ccaaaacagg 119760 tatttaggta gttgttgtct aagccatttg ttgccgattt aagtaaaagt ccatccattt 119820 tctgtgcctg ctaaaattga tacttttaaa gcaagaaaat gaaacaggag gaaattcaag 119880 gtggaggatg tctcggtgga gcaggagggc gtgaagagag agcacacgga agtgaggctg 119940 ttgaccgtgt tctcactgtg ctgggcagtt gagacataga tacttatttg gaaaagaatt 120000 aaactcgagg gactcagtgg aatggaaaaa gtcagctatg ggtggaacac cagaccattt 120060 catgaactgg gacttgtaaa tgttctcatt ctggcacctg ctgagctcct agtgacataa 120120 gagacctaag ctccagattt attccgaatt aacaaattga gccagatcct cagatctcgt 120180 ggaagaacaa gcaggtgctc tcgactaatg agccacctct ccagtcccaa ggtatagagc 120240 tttgatctcc atttttcttt atgtacaaat atttccctag gcattaaagg tgtaatttcc 120300 ataatctatt cagtgtatca ggctgtggtc atactgaata agaggttttc ttcacagaag 120360 catcctgtac attaaataga aagatcaatt ttaaatgaca cctgagctta ctccagtgtc 120420 tctgctaatt tgttgaactt ttccttcata atctctttaa attaattatt aactagccca 120480 aagcagggtg gacttgggca ttatagagta cctggtcaat ttagaaatcg tttagtccaa 120540 gtcattagag gattattacc cttttttttt tcttcagcca gatgggggat gtcataaatg 120600 tacacaatta gggttcttta aattaggctt atggcttgga tgtgaaatgt cccctagaag 120660 ctcacatgct tgattacttg gtccccagct ggtagcactc tttcaggagg ttggacagac 120720 acacagtctc attcatagtt ggtggggcat agggatgaaa cttgagggtt acggatcgcc 120780 tctgattctg tcctggttgc tctgtttcca gatccagtca catgtgagca agctatctca 120840 cactcccgtt gtcataacgg actggttctg tttccatgtg tttctcatgg cggtgatcga 120900 ctttatcctc tgaacctcgt gcaaaccctc catgactcaa gttggtcctc ctattaggtg 120960 tttgccctct gtcatgagaa gagtgacacc catagtaggc tcctttccat ccatctcgca 121020 tgtgccccac ggtcaggttc tgttggtcta aggctgacca tcaactgctg ctgaacatat 121080 atatatagtt gagaaacttt tccagtcata ctttccaaga aataaatagg agcttgagtg 121140 tggtgagctg tagctctcag ctggccactg atgtaattat taatcactgc atgcaattaa 121200 ttgggacctt tcttagacat gtttgccact gatccctgtt tccagggagt ttcacaaatg 121260 ttcccacccc agcctcgtac ctctatttgt tcttttgtaa ctttataaaa attttaatct 121320 atacaagttt gcaatgcatt gatcatatct attcccaccc tcaccttcct ggttatccga 121380 gattctcccc accatatccc tttgaagtgt cagatcttcc tcttctgctc tttataacct 121440 actaagtggt gttcatatgg gcacaggtgt aggaacattc accacagcat gagcaacctg 121500 taagagacca cattcctgaa gaaaactccc ctttctcccc aggggacatc aacggccaat 121560 aactctcagt tatggatggg gtaccttccg tttatcctgg aatgttgact ggatgaatct 121620 gtgtaagcag ccactgctgc tgtgagttcg ttaagtgtaa tggcctcatg ttgaagttaa 121680 gccctggatc ttcctccaag ctccgtgctc ccggaaataa ggctcagact caaaatatat 121740 ttacaaatac cttggccatg taactaggct cttctctgac tagctataac ttgaaataac 121800 cattgattct agcctacatt ctcccatgtg attggcgata tgagctcagg taccaggcat 121860 ctgtcttgtc acatcttttc agtgactctc tctgcctcct tctctcccag aattctttct 121920 cctcccacgg ttcaccttct atttcctgcc taagccatag cccatgggct ttttagctga 121980 caggtgatgc attcatacag tagacgagat gttctgttta tagccttgcc atacccagaa 122040 gacactgtgt cacaacagtc actccccatc ctgacctgca gcccttatga ccttttggcc 122100 ctctctttca cagtgttccc tggggggtgg taatggtggt ggtatgcata tatgtcctgt 122160 tttgagagca ccgcatggtc attccacatt tgttcttaag tggaagaaag catgatgcaa 122220 aacagaagga gtgagctaga ctttaactgg atttagggga ctttataaga gaaagtaaaa 122280 acgtcctcaa gaattcatta aaaatgaaga gcatctcccc catgctcatt tctgttcatt 122340 acaaacgact caactattac atgcaaatat tactctagga aaatagccac ccactgatat 122400 gggtgtggag atctccaaag acaagaggaa gttgaaaagg gctcagatgg ctcctccaat 122460 gtggccttcc agcttatgac aagagaaaca caattgctgg tccagggaca gatgtctgct 122520 gcacttgctt tgttcctggc tctccctctg acccaattta cagcccccct gcagctgtct 122580 cctgcatact ttcccacgtg aagttattta tctttcttaa cagtcaactc gatttattcc 122640 aagagaatgc tcccctcccc cagctctgct ggtaatctat tcctgcacta aattgccatt 122700 gtaatcaaaa ttcctagaat agtttaagtt ggccttctgc agctgcagtc acctctgtca 122760 aagccctggc caagcctcct cagtgccctg gagtgagtgt cattccacat tgctggaaac 122820 cacaccccat ctggctcttc ccgcctttcc aggctccttt tgctgtgtcc ttttatcatc 122880 tctactgaac cactttcctg acaaagctcc aacacggttc ctcctctaga gctaccgacc 122940 tcttcaactc ctatgtggtc attttttttt ctgtccttgg ccatttctta acccattttt 123000 ttaggacatc ctcttctctc cttgaaactt ggtcagatct gccaccatat gtaccactta 123060 ctcaatattt tgatcccgga gctcagttcc gtggtggcta attttgtcaa cagacaacat 123120 ctggaatcat ctaaaactca agttgctggg cacttctgtg aggcagtttt ttaaaatcag 123180 attatttgaa gcaggaagac acatccgaca tccgggcccc actttctgat ggcagcccag 123240 ataacatgac gtggaagaag gacacttggc tttttttttt tttttttttt gcctgcttgc 123300 cccactgtgt ctggcatctc tcctgttgct caggcattcc ttcactattc agaatcccaa 123360 caaagataga aaaccatctg ctctccagga atcctccagg atcccagagc taggtgagga 123420 ctgctgagac acccagcctt gtggactgaa cagctgccag tttagatatt ggtctttcca 123480 tcatgagact gccattgttg aactactcgg accatatcct gtaagccagc cttataaatg 123540 catacacaca cacacacaca tgcacacaca cacatgcaca cacacacaca cacacacact 123600 cacactcaca catgcacgca cgcacacaca cacatgcaca cacactcaca aatgcacgca 123660 cgcacacaca cgcacacaca cacacacaca tacacacatt cactctcagt tctgttcctt 123720 cagagaagcc tgactaaatc aggttcccag cacctatgat gggcagttca caatggcctg 123780 ctaatctagc tctgggagat ctccttgctt cttctcatct ctgcaggcac tgtactcatt 123840 gtgcacacat aaatactaat actaataata atagtaatga aaattataaa ttcatatgca 123900 taccagtttt tattagttgg caacagattg cataaccaca acaccacttc acaaccagaa 123960 aattgacaga ctatactagt catatccagg tttcatagtt ttgccctgtg cttagacatg 124020 catattttca gctattcatt gattgcttgc catactagaa actgacctac acaggctaag 124080 ccagtgctct accgctgaga tgtatcccca gccttctggt tccatctgac ttaagttgcc 124140 caaggtagcg ttgatcccaa tctgtaacac aggcatacct gaaacttgca atccttctgc 124200 ctctgtcacc tgcgtagttg agattttagg ccggtgccac tctgtctgta actttgttat 124260 gtggagagac tcgtgtgcct ctctatgaga tacacttggg aagaaagaac ctgccggtta 124320 agggatggcc tccatcaggt tgcctatggg gatgtgtgtg gtaccttctc tgtccagttt 124380 actacgggga gcatcttccc taggcaggtc gcttaggttg tctaagaaag ctagctgaac 124440 acggagcaaa ccactaaagg gcatccctct gtggtttctg ctttaaggtc ctgctttcaa 124500 tctgtctcct gtcagatgaa acaaaccctt tcctctccaa attgcgtttg gtaatggtgt 124560 ttatcataga aaaagaaaac cgagaaaaag aaaaccacca tagtcaaact tagactcgtc 124620 ccatcagtgg cctcttgtga taccttttag aaccaactgc cacctgtccc ttctcccgac 124680 ctgtgaccag caataatatg tctccagatt tatactttga ttaattttaa gaatgttgca 124740 taaattttat caaacaccat acggaagtct gaaatttccc tccccagcat gattctcttg 124800 atatccacct gagttgttgt gtagaccgac actgttctca ttgctattgg tgagtagtat 124860 ttctgaatgt ggacttggta gtttgtctaa ttgctcgctt gttgtgagat gcttgggtta 124920 tttctagtta ttgaaaatca acatatatga aaatgcatat gtagattttg tgcactttca 124980 cttctcttgg ataaatgcct taggaaaatg ttttctgggt catatggcag gcatatgttt 125040 gatttcctag agacatcaat gcagttttta gactgattgt cccattttac attctcagta 125100 gctgcgtatg agtctgacat ccttgccagt gtttgttgat gtccttaaat tttttgaacc 125160 attctgatag tagggtagga tctctatttt atttatatat gtgagtatgt gtttgtgtgt 125220 gagcatgcgc atttgtgcat gtatgtgtgt atgtgtgtgt gtgtgtgtgt gtgtgtgtgt 125280 gtgtgtgtgt gtgtgtgcat gtgtgtgtgg aagaccatcc tcacccttgg agctggagtt 125340 ataggcaatt gcgaaccaca tgagatggga agtgaactca agtcctctgg cagagtcctc 125400 tgtgccccta accactgagt gacccctcaa ctccttagtg tatcatactg tatgtttact 125460 gagtgtttct atgatggctt atgctgttga tcctcttttc atatactact ttgtcatctt 125520 tatagcctct ttagtggcag atctgtcttt tctaagtttc ctagtctaat attttaatca 125580 ctgagttttg acggttcttt atatgtctta gatagtgcca acctccactc tgtttagatg 125640 agcagaaggt ctatggggtt aaaaggcttt ttcagtcatt tagcaaccga gaaaggaaag 125700 atccaagtct gcccctgtct ttatgcagtc tttaccatgg ctcatctcat atgtagtgtc 125760 ttcccaactc atcaagctac attcatctta gcatacatag tctcccaaac caaaaaccca 125820 taaccagggt atacatcctt ttggtgggga attgtctgaa gtttgtcttc taaagtgtcc 125880 agcctgtgtt agtcattgta tcgccctata tagtcaacag gggtggggat ggaacacaac 125940 acctaagtat ccctttagcc ccgaagagcc ttcagttgtc actcctcatc aaaactattt 126000 aactagagtc atctcattca aattcaagtc cccttgccag attaaaatat agctgatgag 126060 tagttatcat gaaggtaaag aagccagtca cacggctagg gagatggtca atgggtaaag 126120 tgcttgactt gtaaacccta ggacccaaat tcatgttccc agcaaccaca taaagtcaaa 126180 cgtggcatct tgtttctgca agccgaggtg tgaagggtga agaagtgaat cccagggact 126240 tcgggtgggt caatctagtt gaagcgatga gttccaagtt tagtgagaaa caacgtcaaa 126300 aaataaggaa gaaagaaatt gaggaagaca ttgtgatatc atcctctttc gtgtacatgc 126360 acatgggtag gtaagcacgc accactcaca cacacacaca cacacacaca cacacacaca 126420 cacacatcaa ttcttcatag ctctaagtgg attctttttg tcaaagaatc agctgaagct 126480 taatggagat tgcatacctg agctcttgtc ttctacaggg attgtaagag caatgcccag 126540 atgactctta tgtacacagc tgtcccagag tctgttttct aatcactcag cctgtgaaaa 126600 ctggtgccat ccatgacttg aacaggctgg tggtcttggg agccagatca ctttcccagt 126660 gggcactgag gagctccacc tgccaccaga tagcagagta ctgattaggc cttgaaccag 126720 aaatgaccag aaaagcatgt tatagatgag aatagtacag tggatgtcat gttctaggtg 126780 tctgagaaat atggaggaaa atgaccctaa tcataaagaa attatagggt agatattaag 126840 cacgcctaaa ttattaacca gacaataaga ggacatgaga gattaattga aggttaatag 126900 ctcattgtga aaggcagggg cctctctggt agcttataaa gatgctcttg gcttttgcaa 126960 cctgaggcct gagaaattgt ggcctatgac ttaatagtaa gaatggtgga acaacaatga 127020 agggttaaga cctgaagtga ggccatgtca taagttgttg ggccattgtc ctcacccagg 127080 ttaggaaaca atggaatttg acacatatgg caggagtgga ttatgtcaaa aacaataaaa 127140 ctgaattgaa ttaactgtat tggatctaga gttgtttttt tttttttttt ttttttaaat 127200 tctgtgctgt aagcttccag aaaaggcata ctccttcctg ttaaacacca gtgctcttct 127260 gatgtatcag gactgtatta gttacctctc tgtgactgtg agaaaacacc atgacaaagg 127320 caatttgtag aaggacgggt tttactgggt tatagttcca gagagataag agttcattat 127380 ggcaaaaaga gaaggctgga gataggtatg ggagctgagc aggaagcgca aaggccaatg 127440 gcaaacatga agcagagaga acaaattgga agttgggtaa agctcaatgc ttgttgttag 127500 tgatgcactt cctccaggaa agttatagcc ccacactccc atcgcctctc caaacaattc 127560 tgccaacagg ggaccatgtg ttcaaatgcc tgtgggagat agttcacatt caaactacca 127620 caaaaaccat acagtgctcc atgagttagg aaaacatgcc tgctttacca gattctctcc 127680 tggtcaccaa gctaaaccct aaggatatat caccacaacc tggcagagga ctgagcttgg 127740 tctggtaatg gaagaaggta taaaccaaaa ggaacatcag gtcctagcca ttaccataag 127800 tgggactgga ttgtgtaagt tttgcctcaa aggtggcatt attctaagtt agatgatgga 127860 ggatctgttt cttgaggcag ggtctccttg gatacaggag ttaccatcta tcaagatagg 127920 ataaagctgt ggctggcatg aatagaatca tgatagatga tttaaagcct atgtggggga 127980 tggaagcagc ctcccctaaa gtaaaagtgg aaatgcatta ttgtcattgc tatggatgct 128040 ttctgggcaa taaaagacag tgggatatga catacagcta gaagtcttat ttaataattt 128100 ctattatgat gctataatgc aaagcatgga gatacggaca gatacgatga gtactggtat 128160 aattaagaaa ttcagcagta aatagctcct gaagtcattt ctaatcagag atgtcattgt 128220 ggagattgtg gagattgtgg attgtgacac agataggaag atcccaaatt aacagccgca 128280 ggggttccat ttcaaactta gatgccaggt gacagcattt accaaaacag aaggtcaagt 128340 gcaaaagaac aatcaggaga tctgactcca agataacaat ggcaattgtc aacacagctt 128400 cctagcgttg gggagaaaag agatgtcaag agacaataaa accattgcac gcaccagagc 128460 aagaggagcc aagagtgtat gagcaagatc ctagagacaa ggtttctcca cagtgtgttc 128520 tgtcacccag gttcagagct ggcccagttt tcaaacacag aacccaacga tgaagtgatc 128580 aggtgtcctg gaaaaaaaaa aagatttctt caaaaccatg gtgacttaac aggtaacaat 128640 tccctgacac ttccctgaag caatcttcaa tcaccaactc agttagccat aagttatggg 128700 aatgtttggt tccaaacatg tcaacctgac acaggatcat acacagattc aaagtatcaa 128760 catgaacctt ctgttacact gggagcacac ctggccatga atggaagctc agaacagttt 128820 caggattaca atgtgtccac agagtaggta ggctcaacca gtgatcctct ctcctgtccc 128880 agaatgtaac attgaagaag tgcacttggc attgggccca gcttgggttc ctttacctgt 128940 ggatgaaact taatgaggga aacattaata acattattaa cattaataac ataattaaaa 129000 cataatgagg ggttggacag aagcaccaat gagaaccctg ttgctcaaat ctgcccctta 129060 caaaggtata aaatgtgtgt tctttctttg ttctgggtct ctcctctggc ctgcgtgctg 129120 agaggggagt ccccaacatg ttggatcaat aaaaatccaa taaggccaca ctcctaatag 129180 tgccactccc tagcccaagc atatagaaat catcacattc cagtctctga accccagagg 129240 cttgctcaaa catatgggac ataactaaac ctaacataat gcaaagttca tttagtccaa 129300 cttccaaagt cctcatagtc tgtggcggtc tcaacaatgt tttaacttaa tcttttaaga 129360 ccaaagttca aggtctctac tgagatttac tcaatcactt aactgtaatc tccaaagcaa 129420 gacaggaaac caaatgggta aactccaaac tttggatctt catgtctgat gtcaaaactc 129480 tcttcagatc tccaacctct tttttatctt tgttgactgc aacaaacttc tttctcttgg 129540 gctggttcca ctccctgtta gcagatttcc tcagcagata gttcatggct ctggcatctc 129600 aaacatcttg gggtctccaa ggaaaattca atgttacagc ttcttgtttc aatgtctggg 129660 atccacacat aatcttctgg gattctccaa agggctggtg tcacttctcc agcactgccc 129720 tcggtggcat tctaagttca gagtgatcca ctccactgtt gctgctgttc ttggtgatca 129780 tcccatggta ctgtgttgtg tctgctcttg accagcaaga aagacacaac ggccagattc 129840 ttctcaacag cctttattgc aggaatgcct caatgctgct acagggacct agggaaccca 129900 gggaggtctg cttatatact ctccagcaca gggaaaggct tcatggcccc ctgggattgg 129960 tcagtctgcc ggcacctaat ttgcatgcac ccgctccgga ggggttggcg ccagactcgg 130020 acttacatct gcgctgatgt tgtttatgac aatggcacac ctggtgccca gagccatctt 130080 agagttggct tcctacagta ctggtatctc caatactctg gggccttcta ctgcaacgag 130140 gcttcaccaa taacctctca taggttctct ccatggtgcc aagcctcaac ttcttttcat 130200 gaccccttca gtcctgggct gtcaagtgca actgaggctg catcttcacc aatggccttc 130260 catggcctct cagagtgcca agcctcagct gctctttatg ttcccttcat cccttcaaaa 130320 ccagtaacac ctgagtgatg gggatggatg tagatgaggt gaaacccaga acacctctca 130380 ttccctgcaa gggaagaccc atgcctgtag ccagaaatgt taggattaga gcaaaaccct 130440 tagtagaaac tgctgtgatt ctcatttgga agaccatgga ctgatctgtc agcagtgtta 130500 ttggaggtag cctttttctt tctccattgt gaagttgatg gtccttgggc agacttcaag 130560 aaagggtctg aggagacaga cccaggaaga gtgtagcaat aacatttgat ttgcgattaa 130620 gaaggctgga gtcataatta gccctaaatg ggacaactct atcaccctct ccaatgctca 130680 gggaagagga gacagaaaga atgtaaaagc cattctgggc ttgacatagc tgttgtcttc 130740 atgtggtgac ctgcactaga gctgcaggcg actgggcatt catcaagtgt gtgggagggt 130800 cccctgaggc cccacccctc ccttcaggac taacagaagt tagtgcttgc caagggagta 130860 gctatcatcc tctgcagtga cacggaagct gacaagttac ccatgctcca ggaaataaac 130920 tcccacccac gctcaggcag gcgacttacc ttagactgag taagtcacac acaaaagaag 130980 acatgaaaga aggaggggct cttcgttgag actaagggtt tcaagtggga gaaagagaag 131040 gataagacta ttaaaattca ttacatacag gtatggaact gccaaagaag aaaatgatga 131100 agagcagcag ctgccgccac cgggagtgag tcagaggtgg cctgcaagcc tgcaggaacc 131160 tggccctcac caacagaaaa ggcggaggac agacacctga caagagtgtt ctcttccctc 131220 cactaagtgt cttttacagt ttacatgttg atttgggaga ctgttaattc tgggatatgc 131280 ggggggtggg gtggggggaa caactccaac attctggacc aaatttaaag caacctttat 131340 taaatattag atattgacca agggatggac cttagttggg accatttcac agaacttccc 131400 acctgagaat ggcctgagcc atgggttgca ggggcaaagg gcaaacctat aggccactgt 131460 actttcccat gatgctttgc tgtttcctaa gaactataat tcccagcatt gcaggaagtt 131520 actgggtttc tgggcaggta aggcttacag gttaattttg ggcattataa gacaaatacc 131580 aaaatattat ctatgatact attgctttat tttattttat tttcaattac atatgcttgt 131640 atgtgcatga gagtgcaagt gcccacagag gttagaagga ggtattaggt atcctggaga 131700 tggagttaca ggagattatg agctatcata tagatgttgg aattaaacct cagtcctctg 131760 gaagagcagc caatgagcca tatctccagc cctatcaacc tgttgggttg tttaaaaata 131820 attcttttat ttttattttt aagtatgtgt gtgtttgtgt gtgtgtgtgt acatgggtgc 131880 agtaccctaa gagaccggaa gagaacattg gattcccccc ccccccaccg cccccacctc 131940 ccgagctgga gttacaggca gatgtgtccc ttggaaacca aactcagctc gcctagaaga 132000 ggactggact atcgagccta agcgcccatc taagtctttg tatccagtct ttctgcaacc 132060 ctggaagcct ttttaccaaa aagtacattc tttcatccta gctgtttttt gtgactagca 132120 cctgctgaaa aaaaaaaata agaaagaaag aaagaaagaa aaaacccaaa acatcatcaa 132180 aacaacaaaa agccaagccc accaacaaga aacggatata cacaaaagcc aagaaaaaca 132240 aaacaagccc ccccaagccc cagctattca tcaagatagg aaagagaaac acccaactcc 132300 tacctgcgct tggtgattgc ttggtgaaaa ataaaagtat ttttcctctc attccttctt 132360 ttgtgatcaa atgaataatg aagggattga gcctttgcta gtgtgcatgc ccctgcaggg 132420 agccaagaaa caaataggtt tcctaagggt ataacccagc cttttcggct tttccgtcat 132480 cacgtgattg agggcttctg cttcccactt aacctgcatc cttcccagac tgattcatgg 132540 aacctcaagg gctctacagg aaaattcaaa catcagcttc tctctgccat aggacttcca 132600 tccgaaggaa tggttccttg gttgttgcct tcacagccct cacatccggg ctggacctgg 132660 cagaaagttg acatttgacc cctatctaga gcatagacga tgctggcagc cgtatgtcct 132720 cacttttgcc attccggtgc ctccctcggg gcacagccaa ggttccgaga tgggcatttt 132780 caggaggaaa cgattttatt tgaggtcttg gcagggagat gagaggagga ggcgtgccac 132840 cttctgtaac aaaaggcatt tcccagcctt agacacaccc ttaggcccag atgtacactc 132900 ccactcctgt caatccaact gcactgacta gcaaccctaa tgcctgctgc ttccaggcac 132960 cccgcagcct gcgtcttgtt tccactattt cctctgcctg tcgcagggaa agcctcacta 133020 ccaagagcct tcaaacacag ttcctgttac taattttgcc ccaaagggta ccacagtgat 133080 taacttaatg cttccccagg gatgactctc tctgtctggg gtcagggtgg ggctggatgg 133140 attttcttta ttaaggatgg tgggaaccct cttttgagct ttgaagtctc gccttagatt 133200 tttttttaat tttatttttt atttttgata actacaaagg gacaggaagt ctctttttac 133260 tcctctgagg cttgctgttc tccaggggca tggaaaatgt acacgttgtt agccccccaa 133320 gcctgggggc tggggcccgc gctctgccgg attgttttta tatcccagca aaaagcatct 133380 gtgtgcagaa cagcagctgg tctgctgctg ccaactgcac gttcccagta ccaatgtaag 133440 cactttcctt agaggttaaa acccccaagg cactgtttgt gtttggagaa gaaatgctct 133500 aaaaatagta agtgggggtt taaagtctct tcgtcagatg ttcggaagca cgctgacctg 133560 gtgcgctgct tcttagaacc agagctcatt ggcaggttgc tgtgccttgc acctacgatt 133620 cctgactcct tcagtaagct agctggtgaa gagctggaga atctgtattt ataataaatc 133680 cccgggtggg gcattgatgt tgctgatttt aagaacacac actgttgtag ccagtcaaaa 133740 gacccttact cagaaagacc acagagaccg ccgccatcgc tgcaaaccgc atgaggattt 133800 ttattgatcc aacatgttgg ggacacccct ctcagcacgc aggccagagg agagacccag 133860 aacaaagaaa gatcacattt tttatacctt tgtaaggggc agatttgagt aacagggttc 133920 ttattggtgt agcaagtaac cctttcaggc attggttggt ttgtatagtg actaagtaac 133980 cctatggccc agtgactact ttgcttgccc ccatggtggg ttattatgat ttggtcagca 134040 aagtaatagt actttaacat cacagctatt aacgtcaagg gaattctcac aacaagctca 134100 gggtggtttg tggtctttgt cagaattcag tgtggggcag gggtcaggag aattgctaat 134160 cttgttatgg ttacttctct gagaacagct agtattgttt tggtagttgc aagcttaatc 134220 attttgacca ccaaaactat gtttttacat ttgtttagtc agccggcttc cctatctggc 134280 tctgcacgtg gcctgcaagt acagtttgga aagtgttttt cgaaggagga aggtactggg 134340 tggtcttgaa ttcattttgg atattacaac acaaagaacc actggtctgg tgtatttccc 134400 cccagtctgt tgttggctgc agggtgtaat acagacacag ggtttattac cagggagagg 134460 agggtaccat agcttgatgc aattccttat tacctgtgtc gccaagagca agtagcttaa 134520 ccttgctggt ctctgtttcc tcccttcctt gacaatcatt tctatagagc taatttatat 134580 caggatctta attatcttgt tttgtggagg gcacatgttt ataaaacgtt gatctttctc 134640 cctaccaata ttttttctca ttgtcattta aagagtcagt tttgggactg acaagatggc 134700 ttagtgggta gagaaggctt gccaccaacc ctgatggtgg gagctcaatc tttgggactc 134760 acaaggaaga aggagaaaac agactctgat ctgtacacat ttaaattggc acgtgagccc 134820 ttctcctcaa ataaagtcac acacacacac acacacacac acacacacac acagagagag 134880 agagagagag agagagagag agagagagag agagagagag agagagagaa agatacacac 134940 acacatccca tacacatttt ttggccaggt tagacaatgc tggggaaaga tatcagattt 135000 atgcatctct ctctctctgg actgagttgg tgactgtggg aagggatggg acttgactac 135060 tgcaacttgt cagagcacag taggccactg tgaattgcag ctctcagact cctggtacca 135120 tcccatagtc cagcacatgg gagctaaaag tccaaataac aacaatgctg tctgaggact 135180 tggaggcagg tgacattgtg gtagggattt gtggatttcg atgtgtgttt gttctctgtt 135240 tttaacacct tccatcagat aagttcctgt cctgtttctt cagatcccaa cctgttttcc 135300 atttccttgg ggagcctttg aaatatgtgg ctggatctgg atgttatgaa cttgtatgga 135360 gttctgcaca agtctttata gtttcatttg attgcaatcc aattaatgta aggatcctcc 135420 ctgaatgatg cgccctgtga aagcaaactc catgcttaat ctcttcccta tacttgtgta 135480 gtatgcattt cttgactttt catgagacat tcagagacta tcctataaaa gtgtgtaagt 135540 cataaaagtg tgagattaaa aaaaatagca ggtgcaaatg aatcacattt tcttgatcca 135600 ttctttggga aacatctggg ttgtttccag tttcttgcta ttacaaatat agctgctatg 135660 tacctggtag aacaagtatc cctgtggtat ggtggagcat cttttgggta cacgcccagg 135720 agcggtatag ctgggtcttg aggtagaact attcccaatt ttttgagaaa tcaccaagct 135780 gatttccaga gtcgttgtac tgttgtggcc ggccagcagc tcgcaacgtg aacgtgactg 135840 agatggccac tcgagctaag ggaggaacta gtcggggaga aagaagaaac ggagccaaga 135900 caagttcatc ctgttcaaag ctcaatttta ctagttctga cattcagtta taaaggaagg 135960 gggagggaac ccgattcccg ccaactggtc ttggagtcca gtagcagggt gaacacgtgt 136020 gtggctccaa gcagcaaagc agcacggtcc agcagtgggc gtggcagaat gaatgagcgg 136080 gaaactccac ccctgagcca gcaggttcca ggctgggggt ggggaggctg cagttgtaca 136140 agtttgcacc tgcaatggag gaatgttctc ttcgctccac atcctcacca gcatttgcta 136200 actcttgagt ttttgatctt agccattctg atagatgtaa ggtggaatct cagagtcatt 136260 ttatttgtat tttccagatg attaaggaca ttgaacattt ctttaaatgt tcctctgaca 136320 ttaaagattc ttctgttgag aattctctgt ttagctctgt actctatttt ttaagattgg 136380 attatttgtt ttcttggtgt ttaagttctt gagttcttta tttatcagtc ctctgtcaga 136440 tgtagggttg ttgaaggtct tttcccatca tgtaggctgc ctttttctcc tattaattat 136500 gtcccttgca ttatagaagg ttttcagtgt catgaggtcc aatttattaa ctgttgatct 136560 tagcgcctga accatttgtg ttctgttcag aaagttctct tctgtatcag tgcattcaag 136620 gctatcccca ctttctcttc tattagtttt agagtatcca gttttatgct gaggtctttg 136680 atccacttgc acttgagttt tgtgcaggat gataagtatg gatctatttg caatcttcct 136740 catgcagaca tccagtagga ccagcacaat ttgttgaaga tgctttcttt tttccattgt 136800 atggatttgg cttctttgtc aaaaatcaag tgtctataca tgtgtgagtt tatttctctg 136860 tcttcgattc aattacattg ctcagttatt aaaagcaagg acatcatgaa gttttcaggc 136920 aaatggatgg aactagaaaa tatcatcctg aatgaggtaa cccagctcca aaattataag 136980 tagatattag ccataagtat acatacagga tacccaagct ataaaccaga aacccaatta 137040 tgctaaataa caaggagggc tcaagggagg atgcttaatc tcactgagaa agtgtcctag 137100 tcagggtttc tattcttgca caaaacatca tgaccaagaa acaagttggg gatgaaaggg 137160 ctttttcaag ctacacctcc acattgttgt tcatcaccaa aggaagtcag gactggaact 137220 cacacagggc agaaacatgg aggcaggagc tgatatagat gccatgaagg ggtgctgctt 137280 actggattgc tcccctagct tgctcatctt cccttcttat agaagtcagg accaccagcc 137340 cagggatggc atcacccaca atgagccctc ctcgcttgat cactaattga gaagatgcct 137400 tacagctggg tctcatggag gtatttcttc ctcaagggag gctcctttct ctgtgataac 137460 ttcagcttgt gtcaagttgg cacataaaac cagccagtaa aggaggagaa acaaaatagt 137520 cattggaggg gatggaggga aggaactggt tgagagaggg gctggggagg gaaatgggga 137580 gttggtcatc aggtgtaggg agagaagggg aaagggggct gtgagagtga atggaaattt 137640 gtggcaggtg ggagtggggt gggggtgggg aggctgccat atcatttcta ggatgtgtca 137700 gagacctggg acaggggagg ctccaggtag actatgaggg gcctctagct gagattcctt 137760 gtaatggggg atatggagtc tgaaatggtc acttcctgtg gccagacagg acctcccagt 137820 agagggaaaa ggataccaac ccacccacaa aacctttgac cctaaatttg tcctgcctac 137880 aagaagttcc gggacaaaga tggaacagag acaaagggaa cgaccaacca atgaccagcc 137940 caacttgaga cccgtgccgt gggcaggaac taatccttaa gatttaatga tactcttgtt 138000 atgcttgcag acaagagcct ggcatatctg tcctctgaga ggctccacct agcagctgac 138060 tgaaacagat gcagagactc atagccaaac attagacgga acttggaaaa tgtggaagag 138120 ttgagggaaa gattgaggga cacagagagg ataggaactc cacacgaaca acagagtcaa 138180 ctaacctgga ctcttgagag cttccagaga ctgaaccacc aaccgagcat gggctggacc 138240 ttggcatcct gcatttatgt agcagatatg caccttggtc ttcatgagag tccctcaaca 138300 actagagcag ggactgtcct tgaccctgtt aactgcctgc ctgtggatcc tattccaact 138360 gggctgtctt gtctggcctc agtggaagag gatgtgccta gtcctgcaat gatttgatgt 138420 gccagggtgg gttggtaccc agcacccccc tcaaaggaga agaggatgag ggaggagcta 138480 tgtgatgggg gtactgggaa gagaggagag atgcaaattg gaagtaaagt gaataaataa 138540 ataaattaat taatggaaaa aagtagtggg tgctatgaag gagtcatcta tattagattt 138600 tctggagtta taacaaaata cttgagagaa atggagttat tttattatcg ttttggagat 138660 aaaagtccaa ataacagtag tattatctgt ggtaagaact actgtagatg gcactgtgat 138720 aagaatttgt cagttcctat gtctgtaaat gtgtagcgtg tgtgtgtgtg tgtgtgtgtg 138780 tgtgtgtgtg tctgcatgtg catgtatgtg gggtgtctat gtttgagatg gatatggatg 138840 tgctgtgtgt tatatgtatg tgtgatatat gtgctgtgta tgtggaagtt atgtgtgggt 138900 atgggtgggg gctatgtgtg aaagaaagag agaaacagga tagggaaagg aagtcagagc 138960 cagacctata tggggacagt aatgacctcc ttatttagat ttatctctta agggttaaag 139020 atgacattat tttttaacat tgttgctcca ggaccaggct cctaatgcat gcagtctgag 139080 agacacattc aaatcatatc ctatacacag taccatcctt tggccagaat attggtaaaa 139140 ccatccaaat gttagaggag agtagccaac agttctggca tccgtataca gcactgccgg 139200 cgtagagtgc cctactgccc tgaagatgcc ctgtgatttc attgaaaatt ctttccagat 139260 tggtaacctc tcagcccggc tctctgtgcc ttatagatca tgagatctgt aacatacaac 139320 gtaacaatta gcttctcagt tcttgttgga gaggctgggt ttataagaaa gaaagcagcc 139380 aagcacatca gcccacggga gcctggcata aagtgtacct cagcagttcc ttagtgtttg 139440 tagaataaag taatgtatta tagctttcct atagatccac atggaagcct gagaagctat 139500 gtggttctcc tcagacctca tagtctgctc agattcactc acttgggcgg gttgtatagc 139560 tctggggaag atttcacttc tctgcctcag caacacaggc tcaggcgcat cctgctttgg 139620 gttgtgaagt tgaccggaag tgacacgtat agctttcaga aagaagtttg aagagccagc 139680 atgtgactga ccattcgtgc ccttatacct tgttttttcc aaggattatg atagagccac 139740 aggccattgg gtgctgatat ttgttttcat ggtgtaacta aatctgtccg cagagcaaca 139800 cttctttttt atgaagataa gaagtaactg aggcaggttt ccaatctaga catggttact 139860 gtagggtctg ctacttgcta gcttcaccca gctgaatcaa gaagagaaaa caactggcca 139920 agcttgttgg gggcatacat gaaagtctag tacttaggag gtggaggcag gaagataagg 139980 agtggaaggt cagtcttggg tgcatttgtg tgtgtgtgtg tgtgtgtgtg tgtgagagag 140040 agagagagag agagagagag acagagacag agacagagac agagacacag agagagacag 140100 agagagacac agagagagag agacagagag acacagagag agagagacag agagagagac 140160 acacacacac acacacagag agagagagag agagagagag agagagagag agagagagag 140220 atattccctg gaatcaggct cccattacaa gcactaacaa ttccatctga tgccttgcaa 140280 attgtttcag aggcctggga aagataggaa aattttctgc ctatttttag aaagccaatg 140340 taacattgta ccaaaatctt ataaagatag cattgaaaaa aaagaaaacc aacatccgat 140400 ctcattgaag gagtaacaaa tcctaaatta ttaaaataat aacagttcag caaagcatta 140460 aaatatacca agtgaagtcc ccttttgaca atcaacaacg gcaccttacc ccacacacat 140520 tatgactgac tgtgtacccc tctaaaatag gacgaagggg gaaagaatgc attgcttcag 140580 aaaatgaccc agcaacatat gctagttatc tatttatttt tcttgccttg acagaagcta 140640 gctagtatta tctggaagca gggaacaatg tctgttgaga acctgcctct gtccggattg 140700 aactgtcaca aggcaagtct gtgaaggcat tttcttggtt taataattga tgtggtaggt 140760 ccagccctct gtaggtggta ccacactgag tgtgtggtcc tgggtcgcag ggttaaggaa 140820 cctgaacatg agcctggaga ggaagccagg aagcagcact ccttcatggc ctctgctcca 140880 cttcctgctt tgagtttcta gcttgaattc tcttcatgaa aaattattgg ctgggctggc 140940 gagatggctc agtgggtgag aacactgact gctcttccaa aggtcctgag ttcaaatccc 141000 agcaaccaca tggcggctca caatcatatg taatgagatt ggacacgctc ttctggtgtg 141060 tctgaagaca gcagcagcat acttatttat actaataaat aagtctttgg gccggagcga 141120 gcagaggtcc taaattcaat tctcaacacc agatgaaggc ttacaactaa ctatctgtac 141180 agctacagtg tgtaatcata tacataaaat aaataaataa atcttttaaa aattgaaaaa 141240 ttatttggtg taaggtaaag aaacaaaaac ccaaacaccc tttccttttc aagtcgcttt 141300 tagtcgtggt gtttatcaca ggagctggag gccatgtaag atgcgtagga tgtagcattt 141360 agaagccatg taggacacct cgtttagtgg agctaagcca ctagtgtgat ataagagtcc 141420 gacgttaata gacggcttta ccaaggaata atgaattctg tatttcaaac ggagtcggga 141480 ttggcttagg gttaaaaaga acctagaagt caaggttccc caggctgggg tggctctgat 141540 agacgagtgc caagcacagc tgcagatagc gcaaaagtat gacttaaaag atacatgcta 141600 taaggaagaa aagtgaggga aaattgcaaa atatgaaaat agcaagaaat ttcatgtatg 141660 tatcaagttt cccaaacaag ggacaggcac acagtcacac agacacatag acacatagac 141720 acacaggcac acagacacac agacaggata ggatattaag cactgcattt tgttcctctt 141780 gttgaaaccc tatgtagttt cattatgtaa tccaggctgt cctccgactc atgctactcc 141840 ggactcagtt tcccaagtgc tggggtttca ggcacactgc taccttgtcc cactgaaaga 141900 gttgtgcctg agatggtcat tacttagtga aggacagtga gacttcagca atggagatgg 141960 catccttgtt agtggatttg tccacttaaa tgcattagac aaagtctggc ttatcattta 142020 ttattattgt tattattatt attattatta ttattattat tattattatt ttaagcatag 142080 agttagtgat tactcattcc tatactttcc tattgttaag gaaaaaaatc taaaatggca 142140 aaatcacaat gtgaaataca tatgcaaaga tcttaaaacc accagtccgt gagtaaacca 142200 gttccttttg gagacaaagg aataggcagt aaataagaga aaaccacatt tgttaatgga 142260 tgggtagttg ttcactctag ccatggagac actgaatcag cactcgacac gattccttta 142320 catacttaca gaggacattg attcgtttct attgttactg agtagctcca acttcccagg 142380 tcacaagttg ccaagagcag ccaattattc ttaagtttca atttgttgaa aaaaaaaaaa 142440 gccctgatag cattgatgtt catccccgag caactcagta actcagtgtg tgtgtgtgtg 142500 tgtggggggg ggtgtgtgtg tgtgggaggg tatgtctgtc tgtgtgtctg tgtgcatatg 142560 tggacataca tatgccatgt cacatatgtg gaaatcagag gacaacctgc aggagttagt 142620 tctgttcttg aaccatgtgg ggttctggga attcgagtca agccattagg tttggtgtca 142680 agagcttttt cccgttgagc cattttagcc catagaaact ggttctacca caaacattta 142740 caagatacaa aagctaagga cactgcttag tccatttaaa atgatatagg tcccaaagct 142800 tcgcttactg tgggattttc tcctatcatc tcatcccttt ctttcatctg gtctttagga 142860 tccttttctg aaggtgtgaa gttggtagag aagcctactg tgtggacagg aatgtctctc 142920 aaatgcatca ccattttgag tagtagtgat tgagaacaaa gggaattgtt gtaaggtgtg 142980 gagttatttg ataaggtgtg gttgtaacaa agcaccaaaa ctgggccagg gagatagctt 143040 atcaggaaaa gcacttggta cagcctcctg aatgccagag ttggatccct ggagtccaca 143100 gaaaaagccg agtgtggaaa gccagatgca gtgacaggaa tttgtaactt gggcattcct 143160 gggaagagat gaaaggagaa gatgggagaa tggcctgaag cttaagggac agctatggga 143220 tgtgtgctac aagaacagaa actagggaga ctctgcctca aagtggggtg ggaaccaact 143280 acacacaggt atgttcaagc agacaccaca cacacacaca cacacacaca cacacacaca 143340 cacacacaca cacacacaca cacacattat taaagctgat aacagctttt agactaagta 143400 gaaggaaaaa gagataactg tagttaacaa aatctaattc ccaatttgct acctacattc 143460 cctacctgca gtatgctaac tttaagaggg aaaaagtcct ccaaccagag tgtatataaa 143520 atatgttgtc tgatcctccc aatagtggtt ttctaaaagg aatatcatag aagatattta 143580 gaagacttcc ttttacgtta ctttttgaga cagggtttta tgtaagccaa gttgacctca 143640 aactttgaat cccacttctc tacttcctga gagctacagc tataaatatg caccatcatg 143700 gctggtttac atggtgctgg ggacagaacc caggactttg tgatgctagg caagttctct 143760 accaactgag gtcgatcccc agccctcaga taacattgtg tggcgctatt ctgtctaccc 143820 aagagagttt atagtttaga cttccagatc aagcgaaata aagagttctg gggagatgtc 143880 cttttgcttc ctagtgtagg tatagatgtt acgggatggc aggatttagt cagaggaaga 143940 gactcaactt ctctgaaggt cttactatgc atacaacttt ctgctatggt tcttatcctg 144000 tggcataagg acatcccctg ccacccaatt ttacatgcca catcccaaat cattgtaacg 144060 aggcttcaca tggcaaccag cagttgactg caatgagact tccatggcaa ccagcagttg 144120 actgtaacaa ggcttccatg gcaaccagta gttgactgca acaaggcttc catggcaacc 144180 agcagttgac acctgccttg ctctggtttt gttacagctg aactgtttct catctgtgaa 144240 accccttttt aaaacatgta ttcatttatc ttatatgtgt acactgtagt tatcttcaga 144300 cacaccagaa gaggacatca gatcccatta cagatggctg tgagccacca tgtggttgct 144360 gggaattgaa ctcagaacct ctggaagagc agccagtgct cttaactgtt gagtcatctc 144420 tctggcaccc ccgctcccat gaaacccttt tgcatatatc tttttgtctt ctagtcttgt 144480 ggtccacaag ccctacaagt cacttttgga gttcttcctt agccccactc ccacattctt 144540 gctttggatt atcttcgaca ttcagtgctc tcagggcagc tgtgaacctc cttgaaaaat 144600 tttctctctt gtctggtctc taatgatgtg tagacttgac ttactagggg ttcatgtctt 144660 gctctctctt tcatgcagtt caattcatat ttagcatcct aagtcagtga ctatagggaa 144720 aggttttgag cccccttgga agtctctgct gcaagcatag atcttgcaga gtagatattt 144780 aatgaactct tattaaatcc atgcatgaat gaaactcttc atgtgtattt cccaatcttc 144840 tctctacatc attgaaaggg gaataacaga atttaaattc ttcacaattc ctgcaaaggg 144900 agacttctac taagtatgaa atgtgcaagg cttgaatttg taatgaatgt ctgatgtaac 144960 ccagcattcc cagaatcacc acttgggttt tccacaaggg tatgcattcc tcttttatac 145020 aaatgcatgg agggggaggg gttacaggat aggcagaaat gaaagatatt tgttaatttt 145080 ctgttattgt gataaaacac tatgatcaag gaaactaatc gaagaaaaaa gtttatgtgg 145140 ggttatggtg gccgaggatt agagtccata gcttgcaatg gaggttgcag gtaaccagaa 145200 cagaacaggc tcacatccct gtcttcaagc aggaaacaga aagcatgcgg agtggtgtaa 145260 ggcattaaac ctcaaaggcc atcccacagg tcacacttcc tatacctccc caaacagtgc 145320 caccaattgg gaaccaaaca tcagagtctg tggaggacat attcgttcaa accaccacca 145380 cagacaagta gtttattctg tgcacccttc ttatggagga ggctgacaag taccactgtt 145440 tacacacagt aggactgcca cgaacaatat agccatactc actacctgct ttcccggttt 145500 gtggccttcc catacctcac agaactttct tcttcctgcc tgtacatttc actgctgtct 145560 cttgctgtgg agtggggctc tatatgtgcg agggtctcag tgtgaagcag gctctgtgct 145620 acttgtctta tcctgagtta cagagattat tttttgtccc agtccctatc aggagtcact 145680 attttgagcc caatctggta gctctggttt cttctttcca gaatattctg agtcgcgcag 145740 cctcaaaccg gctctgcctt tcctctacat gcctgtgatg acgactgcct ggctgcagat 145800 gctccgggtg ttttcccaca ccagtttctc ttgttgtgac tccatcaaac ccaggtcagc 145860 ttctgtaatc gagacgatcc acttaattaa ctgtaatgag gctcccatca tttccaataa 145920 atttctttcc aaaacaccac ttgagaggca catagcttcc tatcagctgt aaattttttt 145980 tttcctgtct atttagttcc aggaattgaa tttactcaga gatgtcattg cccaacctca 146040 tgtctcaatg ttttaaacta aatctttcag tctggtgcta tgggctacag gacccaaccc 146100 cagtttccct tgtacagaat ggaagatgcc gtgcaaacag cttgtctggg tttcaccaga 146160 gctgggagaa tcagtgctgg ttttcatggc ctcttctgcg atcagcccct tctccaccaa 146220 cagttttgtt agttagacag agcaacttgc gcttgaagat ctcaaaccat ttatcgaagc 146280 atgccatctt ctccttttta aagacctgaa atgctgttgg aataattttg ctgtgtttta 146340 taaaaatata gagaggatta atacgtttgg tagaggtgca ataaggtgtg gagggaaggg 146400 gtgcctccaa aggcctgtgc tgaggcacct cttccccctg agcgaccagc caaagaatgg 146460 tatagtatag aatagagttt attcatggca tggggagggg agttgagaga gtagtagaga 146520 cagagaaaga gagagggaga gagagaaaga gagagagagg ggggggaggg agggaggaag 146580 ggagagagag agagagagag agagggaggg agagagagag agaagagtag tagaggctgg 146640 tcatgagcat gtggagagag agggggaggg gaatggggaa tgggggagca ggagggagag 146700 gacagagtcg gagcaagaaa gcaagagaga acagaggggc taagcagtcc cccttttttc 146760 attttaaaaa ttttattaga tattttcttt ttttacattt caaatgcttc ctataccctc 146820 ccctcgccct gctcccctac ccacccatag cagccccttt tatagtgagt caggcacacc 146880 tggctgttgc caggtaactg gggcagagcc tatacaaaaa tgctaacaga tttgtcagtg 146940 aaatgatatt ttgctctttg acttatagaa gatgaaaaag aaaaccacaa gtttcatact 147000 gaccctttgg ggaggataca tctttaagaa attatgggag agaatagttt catttagaaa 147060 gaatttaagt gttttttttt cccctctcag acatgtcatg gtttaatatc agttatccaa 147120 tttttctttg tgctactctc agaattcttc ctgagaaatt agctttaatc ttctgctttg 147180 tttcaaagcc tgcactagat taagcctcgt cctctctctc tctcctctct ctttctcttt 147240 ggcttcattc gggaatgact aattagccag gagaaattaa atttacaaat taaacctctc 147300 tgcatagatt ttctcaagac cccagccctg ggggtaatat ccatggcctc tcctacagag 147360 cttttgcctc ccagagggtt gtatagaggg gtgaaatgtt tatgaagcca catcggcttg 147420 aagagcaggt tggtggttgg cggttggcag cattataagt tgttctttgg ttccttcagg 147480 gaaaaacttc agtgttccaa agttcagggt catggaagga gggtgaggaa tgttcccagc 147540 cagagaagaa agtgatgtgt gtgagagaga actgccgaga actgtagaac tgaagtttcc 147600 accgctccaa ttacatgcct gaatgccttc ttttcatttc atctctgttt attatctttg 147660 tctgtgtgtg gatggtgtat atatggtatg tgcatttatg tgtgtgagtg tacacttatg 147720 catataaatt caagtgctca tgttttactc cacgtataaa gagatcagag gacagccttg 147780 ggtattgttc attgcctttc acctttctct ttctctctct tgaatacacc acctatcatt 147840 gtaggagact ggcatcacag acctgccatg gctggctttg tatgggatgc agggatgtga 147900 atccaggtcc tgatgtctat gtggcataga cacttaacta ggaccccaac ccattcacct 147960 cccaagcctg actctgctcc cctgctccac tctacaacat gccctctact cgataaaggc 148020 actaaaatgt gtgactaggg gccatccagt ttgttctgag tgtcagctca tgcctccttc 148080 ttaggaagca tttcaagcca atgtctctta ataccaccca cggttcattg ttttctggct 148140 tctccttcct tccttccttc cttccttcct tccttccttc cttccttcct tccttccttc 148200 ctcccttcct cccttcctcc cttcctccct tcctcccttc ctcccttcct ccctttctcc 148260 cttccttgct ttttctttct aacatctttt gtgtattgaa aatgctgatg tttttgctat 148320 ttggggatga gctcttgcag gatagtgttc cccaactctt ctcttccaag tttattctcc 148380 agatctttgc attaagttag gcaggctcct ttagggacag agaccgacgg cttactggag 148440 gggaaggctt cagtcccatt aaaagatggt taatgaacat tcaaagttag tgtttctctt 148500 tgtttcagct gcagatcttt tggagaatct taggagaaag cagccaagac cactgaatcc 148560 acctgacctc attaacctgg agcaaactgg gcttattaga atttagattg aaggcctcag 148620 aggttagata ttgctcttgg agaagaccta ggttcagttc ctagcaccca cataggtcgg 148680 attacaattg ctgataactc cagttccagg ggatctgtca ctttcttctg accttccagg 148740 tcttcatgca cattgtgcac atacagacaa gcacacgcac acacacacac acacacacac 148800 acacacacac acacacacac aaatattaaa actctggagt gtgaacttag tatgcccata 148860 ggtaattatg tccattagct ttcctcattg gacattttct ctgtcacctt taactcagga 148920 tgctcacaaa tatccctacc cctctacgta cttcctttat gaatttgtat atgccccttc 148980 ccttgtttgg ggagcagatg tttctctact ctttgatctg actgttgtgc tcactggttt 149040 catactcacc aagagacctg attcgactta ggtaccgagt cacccacgtc tctcagggtc 149100 agcctgctct gtctctcagt ttttggtcta agaaaacaag gccttccacc cttttgatca 149160 gcagatatga gtaaaagaaa taaaagttga tgctctttgt tttccaggga ttcagataaa 149220 atgtttgcca tatgcatgaa ataattttta agaggtaggt tggttcttcc ccctaaagca 149280 aggtttatct aagaagagtg cttttaagga tggtcgtgat gctgacagta cctcgcattc 149340 ctaccgtcat ttctggaaaa gccattttaa tttttcatct cacctcgcca tctgggagac 149400 aagtgaactc aatgttttcc ttgccattta taaagtgagt tagttaagac tcgggtaaga 149460 ctattgatct gcaccaagat ccaataccag gaaaagagat gaaattggta tccaaacttt 149520 tgtttcagga ctctgagccc tggcctctat tgtctcaaac acagttcaat cataaactga 149580 gtcaatacat ctagcactgc ctggcagtat aaggcaatta aactatcaca aggctatggg 149640 ggtaaagaca ctatcacaaa ggtctgggat aaataaactt gaacaggttg ataaagtgtg 149700 ggactcaatc tctaaaggag gtgctgtaat ctctctgtag tggaaggttt acctgaagga 149760 acagatttga agttattcct gaaagcatac taaaaccaga agacagtttg gcagtccgaa 149820 tcaaaaactt taactaaaga catgaaccac agtactttcc agcatatcac tcagtggctc 149880 taaagttctt ttccttgttt gaaaacaata gagtccaggg gaggattaaa tggtgatttg 149940 taaagtgcca ggctcagggc acattcagta aatgttacca gtgattgcag aataatggtg 150000 ataatatttt cctttgtagg aggaaataat gagcacgtag atttagcaac acacagatcg 150060 tttgttgtgc ttttaaaagt cacagtgaaa agttggaaac aacttgatgt ccagcaagag 150120 ggaattgcag aagagagttt cctggtattt ctaggtgcta gatcattaga tagccatgta 150180 aaagaataaa aggaaataaa tatgttattg cctagcaaga tggccatgaa tttgttgttg 150240 ttgttgttat taagacaggg tttctctctg tagccctggc tgacctagat ctatagacca 150300 ggctggcctc aaactcaaag atcgatctgc atctgcctcc tgagtgctgg ggttaaaggc 150360 ttccaccact gctgcctggc tataaatgtt taaaatatat tttctattaa ttcttcacaa 150420 attccatata tgtgtacagt gtattttgac catattcacc cctactcctc ttcccaaccc 150480 ctttcagctc ccctgctgcc atatttccat cccttaccaa ttttgtgtac tatttaatta 150540 atagatatat acatatatat atttaattct gatatatcag cacattatat attatattat 150600 attatatgtt acatatatta tctgttacat aatatacatg tatacatgta tgtatatata 150660 tatatatata tatatatata tatatatata tatatatata catattgatg tacacccatt 150720 cattggagtc tggatgcctt gccagggacc actcttaagc aaagtggcct ctccctgatc 150780 tggaagtccc ttgactgtta acagatcctt agctagggct ggagacttgg gagccccatg 150840 cctctctaag caggaatcct gacttggtct tgggctgcca ctgtgagctc atgaggcagt 150900 gttgggggag ggatgcttta taccatcccc tttgtgactg agaattccac aaatgctcat 150960 tctctgcact tggcagtgat tttttacctt tcaaaaacac ttataaaata ttaaatcctt 151020 tttttttaag tttgtgcttt tttgatgttg tttaacacat atattcatgt acagatacag 151080 gatagcttta aaaactagga gatgaatttg cagctctatg tcaacaaaac tgacatttaa 151140 aaattgtttt tattaaaatt ctttttattt aaactatccc agtaacaaag gcaagtatca 151200 tgtaaaataa aattgtgctg ttttgttttg tttctggctt tcttgtttcc tactctttcc 151260 tttggtttgt cagctctgtg ctaccataag cagacacgat ccattcaata gttgactaac 151320 agcattcttt tgagtgttta ctgtggccaa ggccctggtt ctaagcactt gatgtacaga 151380 taaaccagat gaacaacaat gcccttcaaa agcttaaatt caaggctgga gaaatggctc 151440 agtggttaag agcactgtct gctcttccag aggacccagg tttgactccc agctcccaca 151500 tggcagctta taaccatcta cagctccagt cctgggaggt ctgacactct ggtacccacg 151560 tggtacatat aaatatatgc aagcgagaca tgtgcacata taattgaaat ataggtatag 151620 tgatcttggg attccagtgt ctgaagcagg agaaggatga gactgagatc agcctggaat 151680 aagatcctct tttgattaaa acaaaagcat gtactaggtg acatgaacag cacaaccgca 151740 agagaaggag agtagaccaa aatttctaga ggagctatgc cttcttaaca gcattcaacc 151800 cctcttctga gaatccctgc agactgccct ccagcatcac tgatttccat agaggctgca 151860 tagctgtcaa agttcacagt ctactcagag gtaacctgct gtgtcaatat ttgtaatttg 151920 tacttcatgg tgagtcactt ccagagaata gattatctac catggaaggg aaaaggtctg 151980 ggagtgaaag tgagctggac aagacacagg tggaggcatt aggtttcaac agcttcctgc 152040 ctctacaaag caaagggcag gaatgtgtgc tggcgtgggc tggagcccag aacagccaca 152100 actctcaagt ggaggataag cagaaagcag ggttagtctc ctgtttctcc aggtggttct 152160 agctgattct tctaggtctg gaaacaaatg ttaccttcta tccattttct ctccttctcc 152220 cctggcttcc atttttatag ctcagaagca aatccatctt ttaagagttg ttctcaagca 152280 atctttgtct aaagctggat cctcaccata ctacatccca cttcaccccc acctccaccc 152340 tcactgccaa ttgaggaagt tctctgaatt ctgacacctc catagcatgt gatagtcccc 152400 tatgttattc ctatgcatca attcattttt acattgtgct ggagaactcc atctctctta 152460 caagagtaga tgatcattaa ggtttggtct atagaactcc aaatagactg gactagaaaa 152520 gaaattcctt ctgacacata ataatcagaa caacaaatgc actaaataaa gacagaatat 152580 tgaaagcagt aagggaaaaa ggtcaagtaa catataaagg caggcctatt agaattacac 152640 cagacttctc accagagact atgaaagcca gaagatcctg gacagatgtt atgcagaccc 152700 taagagaacc caggccaggc tactataccc agccaaactc tcaattacca tagatggaga 152760 aaccaaagta ttccatgata aagccaaatt cacacaatat ctttccacaa atcgagccct 152820 tcaaaggata ataaagggaa aacaccaaca caaggacaga aactacaccc tagaaaaagc 152880 aagaaaataa tctttcaaca aacctaaaag aagactgcag caagaacaga atccaaactt 152940 taacaacaaa attaacagga agcaacaatt acttttcttt gatttctctt aacaccagtg 153000 gactcaattg cccaatgaaa agacatagac taatagactg gctacacaaa caggacccaa 153060 cattttgctg cttacaggaa acccacctca gggaaaaaga cagacactac ctcagagtga 153120 aaggctggaa aacaattttc caagcaaatg gtctgaagaa acaagctgga gtagccattc 153180 taatatcgaa taaaatcgac ttccaaccca aagttatcaa aaaagacaag gaggggcact 153240 tcatactcat caaatataaa atctactaag gtaaattctc aattcttaat atctatgctc 153300 caaatataag ggcagccaca ttcattaaag aaactttagt aaagctcaaa gcacacattg 153360 cacctcacac aataatagtg ggagacttca acacctcact ctcaccaatg gacagatcct 153420 ggaaacagaa actaaacaga gacacatgga cactaacaga agttataaaa caaatggatc 153480 taatagatat ctacagaaca tcttatccta aaaaaaagga tataccttct tctcagcacc 153540 tcatggtacc ttctccaaaa ttgaccatat aattggtcac aaaacaggcc tccatagata 153600 caaaaatatt gaaattatct catgcatcct acctgatcac cacggactaa ggctgatctt 153660 caataacagc ataaataata gaaagccaac attcacgtgg aaactgaaca agactctact 153720 caatgattcc ttggtcatag atgaaataaa gaaagaaatt aaaaactttt tagagtttaa 153780 tgaaaatgaa gccacaacat acccaaactt acgggacaca atgaaggcag tcctaagagg 153840 aaaactcata gccccaagtg cctccaaaaa gaaactagag agagcataca ctagcagcct 153900 gcagcacacc tagaagctct agaactaaag gaagcaaatt cacccaagag gagtagatgg 153960 caggaaataa tcaaactcag ggctgaaatc aaccaagtgg aaacaaaaag aactattcaa 154020 agaatcaacc aaaccagaag ctggttcttt aagaaaatca acaagataga taaaccctta 154080 gccagtctaa ctagagggca cttgtatcca gtctaactag agggacagta tcctacttaa 154140 caaaatcaaa aatgaaaaag gagacaacaa cagatcctga ggaaatccaa aacatcatca 154200 gatcctacca caaaagtcta tactcaacaa aactagaaaa cctggatgaa atggacatct 154260 tcctaggcag ataccaggta ccaaagttaa atcaggatta gattaacgat ctaaacagtc 154320 ccatttctcc taaagaaata gaagcagtca ttaataggct cccaaccaaa aaaaaaaaaa 154380 aaaaaaacca ggaccagatg ggtttagtgc agagttctat cagaccttca aagaagatct 154440 aattccaact ctcctcaaac tattccacaa aatagaaaca gaaggtactc taccaaattc 154500 attctatgaa gccacaatta ctcttatacc taaaccacac aaagatccaa caaagaaaga 154560 gagcttcaga ccaacttccc ttatgaatat ccatgcaaaa atactcaata aaatccttgc 154620 aaaccgaatc taagaacata tcacaatgat catccatcat gatcaagtag gcttcatccc 154680 agggatgcag ggatggctta atatacggaa atccatcaac ataatccact atataagcaa 154740 actcaaagac aaaaaccaca tgatcatctt gttagatgct gagaaagcat ttggcaaaat 154800 ccaacacccc ttcatgataa aagtcttaga aagatcagga atttgaggcc catacctaaa 154860 cataataaaa gcaatataca gcaaaccagt agccaacctc aaactaaatg gggagaaact 154920 caaagcaatc ccactaaaat cagggactag acaaggctgc ccactttctc cgtacttatt 154980 caatatagta cttgaagtcc tagccagagc aattagacaa caaaaggaga tcaaggggat 155040 acaatttgga aaggaagaag tcaaaataat cactatttgc agatgatatg atagtgtata 155100 tataagtgat cttataaatt ccaccagaga actcctaaac ctgataaaca ccttcagtgc 155160 agtagctgga tataaaatta actcaaacaa atcaatggcc tttctctaca caaaggataa 155220 aagggctgag aaagaaatta gggcaacaac acccatcaca ataggcacaa ataatataaa 155280 ataccttggt gtgactctaa ctaaggaagt gaaatatctg tatgataaga acttcaagtc 155340 tctgaagaaa taaattgaag aagatctgag aggatggaaa gatcttccat gcaggattaa 155400 ttggcaggat taatatagtc aaaatggcta ttctgccaaa agcaatctat agattcaatg 155460 caatccccat aaaaattcca acataattct tcacttagtt agaaagggca atttgcaaat 155520 tcatctggaa taataaaaaa cataggatag taaaatctat tctcaacaat aaaacaacct 155580 ctggtggaat caccatacct gaccttaagc tgtactacag agcaattgtg atgaaaactg 155640 cctggtactg gtacaatgac agataggtag atcaatggaa tagaattgaa ggcccagcaa 155700 tgaatccaca cagctgtggt cactggatct ttgacaaagg agctaaaacc atccagtgga 155760 aaaaaggcag cattttcaac aaatggtgct ggcacaattg gcggttacca tgtagaagaa 155820 tgggaattga tccattcttt tttttttttt ttttccattt tttattaggt atttaactca 155880 tttacatttc caatgctata ccaaaagtcc cccatatcca cccaccccca ctcccctgcc 155940 cacccactcc ccctttttgg ccctggtatt cccctgtact ggggcatata aagtttgcaa 156000 gtccaatggg cctctctttc cagtgatggc cgactaggcc atcttttgat atatatgcag 156060 ctagagtcaa gagctccggg gtactggtta gctcataatg ttgttccacc tatagggttg 156120 cagatccctt tagctccttg gctactttct ctagctcctc cattgggagc cctatgatcc 156180 atccattagc tgactgtgag catccacttc tgtgtttgct gggccccggc atagtctcac 156240 aagagacagc tacatctgcg tcctttcaat aaaatcttgc tagtgtatgc aatggtgtcg 156300 gcgtttggat gctgattatg gggtggatcc ctggctatgg cagtctctac atggtccatc 156360 ctttcatctc agctccaaac tccgtctctg taactccttc catgggtgtt ttgttcccaa 156420 atctaaggag gggcatagtg tccacacttc agtcttcatt ctccttgagt ttcatgtgtt 156480 tagcaaatta tatcttatat cttgggtatc ctaggtttgg ggctaatatc cacttatcag 156540 tgaatacata ttgtgtgagt ttctttgtga atgtgttacc tcactcagga tgatgccctc 156600 taggtccatc catttggcta ggaatttcat aaattcattc tttttaatag ctgagtagta 156660 ctccattgtg tagatgtacc acattttctg tatccattcc tctgttgagg ggcatctagg 156720 ttctttccag cttctggcta ttataaataa ggctgctatg aacatagtgg agcatgtgtc 156780 cttcttacct gttggggcat cttctggata tatgcccagg agaggtattg ctggatcctc 156840 cggtagtact atgtccagtt ttctgaggaa ccgccagact gatttccaga gtggttgtac 156900 aagcctgcac tcccaccaac aatggaggag tgttcctctt tctccacatc ctcgccagca 156960 tctgctgtca cctgaatttt tgatcttagc cattctgact ggtgtgaggt ggaatctcag 157020 ggttgttttg atttgcattt ccctgatgat taaggatgtt gaacattttt tcaagtgctt 157080 ctctgccatt cggtattcct caggtgagaa ttctttgttc agttctgagc cccatttttt 157140 aatggggtta tttgattttc tgaagtccac cttcttgagt tctttatata tgttggatat 157200 tagtccccta tctgatttag gataggtaaa gatcctttcc caatctgttg gtggtctttt 157260 tgtcttattg acggtgtctt ttgccttgca gaaactttgg agtttcatta ggtcccattt 157320 gtcgattttc gatcttacag cacaagccat tgctgttctg ttcaggaatt tttcccctgt 157380 gcccatatct tcaaggcttt tccccacttt ctcctctata agtttcagtg tctctggttt 157440 tatgtgaagt tccttgatcc acttagattt gaccttagta caaggagata agtatggatc 157500 gattcgcatt cttctacacg ataacaacca gttgtgccag caccaattgt tgaaaatgct 157560 gtctttcttc cactggatgg ttttagctcc cttgtcgaag atcaagtgac cataggtgtg 157620 tgggttcatt tctggatctt caattctatt ccattggtct acttgtctgt ctctatacca 157680 gtaccatgca gtttttatca cagttgctct gtagtaaagc tttaggtctg gcatggtgat 157740 tccgccagaa gttcttttat ccttgagaag actttttgct atcctaggtt ttttgttatt 157800 ccagacaaat ttgcaaattg ctccttccaa ttcgttgaag aattgagttg gaattttgat 157860 ggggattgca ttgaatctgt agattgcttt tggcaagata gccattttta caatgttgat 157920 cctgccaatc catgagcatg ggagatcttt ccatcttctg agatcttctt taatttcttt 157980 cttcagagat ttgaagtttt tatcatacag atctttcacc tccttagtta gagtcacgcc 158040 aagatatttt atattatttg tgactattga gaagggtgtt gtttccctaa tttctttctc 158100 agcctgttta ttctttgtat agagaaaggc cattgacttg tttgagttta ttttatatcc 158160 agctacttca ccgaagctgt ttatcaggtt taggagttct ctggtagaat ttttagggtc 158220 acttatatat actatcatat catctgcaaa aagtgatatt ttgacttcct cttttccaat 158280 ttgtatcccc ttgatctcct tttgttgtcg aattgctctg gctaatactt caagtactat 158340 gttgaaaagg tagggagaga gtgggcagcc ttgtctagtc cctgatttta gtgggattgc 158400 ttccagcttc tctccattta ctttgatgtt ggctactggt ttgctgtaga ttgcttttat 158460 catgtttagg tatgggcctt gaattcctga tctttccaaa acttttatca tgaatgggtg 158520 ttggatcttg tcaaatgctt tttctgcatc taacgagatg atcatgtggt ttttgtcttt 158580 gagtttgttt atataatgga ttacattgat ggattttcgt atattaaacc atccctgcat 158640 ccctggaata aaacctactt ggtcaggatg gatgattgct ttaatgtgtt cttggattcg 158700 gttagcgaga attttattga ggatttttgc atcgatattc ataagagaaa ttggtctgaa 158760 gttctctatc tttgttggat ctttctgtgg tttaggtatc agagtaatag tggcttcata 158820 aaatgagttg ggtagagtac cttctacttc tattttgtga aatagtttgt gcagaattgg 158880 aattagatct tctttgaagg tctgatagaa ctctgcacta aacccatctg gtcctgggct 158940 ttttttggtt gggagactat taataactgc ttctatttct ttaggtgata tgggactgtt 159000 tagatggtca acttgatcct gattcaactt tggtacctgg tatctgtcca gaaatttgtc 159060 catttcgtcc aggttttcca gttttgttga gtatagcctt ttgtagaagg atctgatggt 159120 gttttggatt tcttcaggat ctgttgttat gtctcccttt tcatttctga ttttgttaat 159180 taggattttg tccctgtgcc ctttagtgag tctagctaag ggtttatcta tcttgttgat 159240 tttctcaaag aaccaactcc tcgtttggtt aattctttga atacttcttc ttgtttccac 159300 ttggttgatt tcacccctga gtttgattat ttcctgccgt ctactcctct tgggtaaatt 159360 tgcttccttt ttttctaggg cttttagatg tgttgtcaag gtgctagtat gtgctgtctc 159420 tcgtttcttc ttggaggcac tcagcgctat gagtttccct cttagaaatg ctttcattgt 159480 gtcccatagg tttgggtacg ttgtggcttc attttcatta aactctaaaa agtctttaat 159540 ttctttcttt attccttcct tgaccaaggt atcattgaga agagtgttat tcagtttcca 159600 cgtgaatgtt ggctttccat tatttatgtt gttattgaag atcagtctta ggccatggtg 159660 gtctgatagg atacatggga caatttcaat atttttgtat ctattgaggc ctgttttgtg 159720 accaattata tggtcaattt tggagaaggt cccgtgaggt gctgagaaga aggtatatcc 159780 ttttgtttta ggataaaatg ttctgtagat atctgtcagg tccatttgtt tcataacttc 159840 tgttagtttc actgtgtccc tgtttagttt ctgtttccac gatctgtcct ttgaagaaag 159900 tggtgtgttg aagtctccca ctattattgt gtgaggtgca atgtatgctt tgagctttac 159960 taaagtgtct ctaatgaatg tggctgccct tgcatttggt gcgtagatat tcagaattga 160020 gagttcctct tggaggattt tacctttgat gagtatgaag tgtccctcct tgtctttttt 160080 gataactttg ggttggaagt cgattttatc cgatattaaa atggctactc cagcttgttt 160140 cttcagtcga tttgcttgga aaattgtttt ccagcctttc actctgaggt agtgtctgtc 160200 tttttccctg agatgggttt cctgtaagca gcagaatgtt gggtcctgtt tgtgtagcca 160260 gtctgttagt ctatgtcttt ttattgggga attgagtcca ttgatattaa gagatattaa 160320 ggaaaagtaa ttgttgctcc cttttatttt tgttgttaga gttggcattc tgttcttgtg 160380 gctgtcttct ttttggtttg ttgaatgatt actttcttgg ttgttctagg gcgtgatttc 160440 cgtccttgta ttgcttcttt tctgttatta tcctttgaag ggctggattc gtggaaagat 160500 attgtgtgaa tttggttttg tcgtggaata ctttggtttc tccatctatg gtaattgaga 160560 gtttggccgg gtatagtagc ctgggctggc atttgtgttc tcttagtgtc tgtataacat 160620 ctgtccaggc tcttctggct ttcatagtct ctggtgaaaa gtctggtgta attctgatag 160680 gccttccttt atatgttact tgacctttct cccttactgc ttttaatatt ctatctttat 160740 ttagtgcatt tgttgttctg attattatgt gtcgggagga atttcttttc tggtccagtc 160800 tatttggagt tctgtatgct tcttgtatga tcatgggcat ctcttttttt atgtttggga 160860 agttttcttc tattattttg ttgaagatat tagctggccc tttaagttga aaatcttcat 160920 tctcatcaat tcctattatc cgtaggtttg gtcttctcat tgtgtcctgg attacctgga 160980 tgttttgagt taggatcctt ttgcattttg tattttcttt gactgttgtg tcgatgttct 161040 ctatggaatc ttctgcacct gagattctct cttccatttc ttgtattctg ttgctgatgc 161100 tcgcatctat ggttccagat ctctttccta gggtttctat ctccagcgtt gcctcgcttt 161160 gggttttctt tattgtgtct acttcccctt ttagttctag tatggttttg ttcatttcca 161220 tcacctgttt ggctgtgttt tcctgctttt ctttaagagc ctgtaactct ttagcagtgc 161280 tctcctgtaa atctttaagt gacttatgaa agtccttctt gatgtcctct atcatcatca 161340 tgagaaatgt ttttaaatct gggtctagat tttcggttgt gttggggtgc ccaggactag 161400 gtggggtggg agtgctgcgt tctgatgatg gtgagtggtc ttgatttctg ttagtaggat 161460 tcttacgttt gcctttcgcc atctggtaat ctctgaagct agctgtttta gttgtcactg 161520 ttaagagctt gttcttcagg tgactctgtt agcctctatg agcagacctg gagggtagca 161580 ctctccttag tttcagtggg cagagtattc tctgcaggca agctctcttc ttgcaaggca 161640 ggtacccaga tatctggtgt tcgaaccaga ctcctggcag aagttgtgtt ccactcacta 161700 gaggtcttag gatcacgtgt ggaatcctgt gtgggccctt gcgggtgtca ggcgactcag 161760 ctggcaaggt agccggggct cgagtggagt ggaaggggtt tgtgccccag atcaagcccg 161820 ggtagcctgc ttccctatgt accgcagtct caagttccac gcgattggat tggggtaggc 161880 gctgtgttcc actcaccaga ggtcttaggg tcccgtgggg agtcccgtgt ggacccttgc 161940 gggtgttggg caagactctg ctggcaaggt agcccggggc tcgagtctcg agtcgagcag 162000 aagggacttg tgccccagat caggcccggg tagcctgctt ccctatgtac cgcagtctca 162060 agttccgcgc gattggattg gggcaggcac tgtgatccac tcaccagagg tcttagggtc 162120 ccgtggggag tcccgtgtgg acccttgcgg gtgttgggca agactctgct ggcaaggtag 162180 cccggggctc gagtctcgag tcgagcggaa gggacttgtg ccccagatca ggcccgggta 162240 gcctgcttcc ctatgtaccg cagtctcaag ttccgcgcga ttggattggg gcaggcactg 162300 tggtccactc accagaggtc ttagggtccc gtggggagtc ccgtgtggac ccttgcgggt 162360 gttgggcaag actctgctgg caaggtagcc cggggctcga gtctcgagtc gagcggcgaa 162420 ttgatccatt cttatctcct tgtacaaagc tcaaaagtaa gtggattaaa gacctccata 162480 taaaagcaga gacactgaaa tttatagagg agaaagtggg gaaaagcctc gaagatatgg 162540 gcacagggga aaaattccta aacagaacag caatggcttg tgctgtaaga tcaagaattg 162600 gaaaatggaa cctcacaaaa ttgcatagct tctgtaaggc aaaagacacc gtcaataaga 162660 caaaaaggcc accaactgat tgggaaagga tttttaccaa tcctaaatct gatatgggac 162720 taatatccaa tatatacaaa gagctcaaga agttagactc ccgaaattca aataatccca 162780 ttaaaaacgg ggtacagagc taaacaaaga attctcaagt gaggaatacc aaagggctga 162840 gaagcacctg aaaaaatgtt caacatcctt aatcatcagg gaaatgcaaa tcaaaacaac 162900 cttgagattc cacctcacac cagtcagaat ggctaagatc aaaaattcag gtgacagtag 162960 atgctggcga ggacgtggag aaagaggaat aatcctccat tgctggtagg attgcaagct 163020 tgtacaacca ctctggaagt cagtcttgtg gtttctcaga aaattggaca tagtactgct 163080 ggaagatcca gcaatacctc tcctgggtat atacccagaa gatgttccaa ctggtaataa 163140 ggacacatgc tccactattt tcatagcagt gttatttata atagccagaa gctggaaaga 163200 acccagatgt ccctcaacag aggaatggat aaagaaaatg tggtacattt attcaatgga 163260 gtactactca gctatttaaa acaatgaatt tatgaaattc ttgggcaaat ggatggatct 163320 ggaggatatc atccttagtg aggtaaccca atcacaaaag atttcaccag atatgcactc 163380 agataagtgg atattaacct agaaacttag aatacccaag atacaacttc caaaacacaa 163440 gaaaatcaag aagaaggaag accaatatgt ggatacttag aataaggaac aaaatagcca 163500 tgaaaggagt tatagagatg aagtttagag ttaagacaaa agggtggact atccagagac 163560 taccccatct tgggatccat cagccactaa acccagatac tattgcatat gccagcaaga 163620 ttttgctgaa gggaccctga tatagctgtc tcatatgagg ctctgccagt gcctggcaaa 163680 tacagaagtg gatactcaca gtcatctatt ggatggaaca cagggcctcc aatggaggag 163740 ctagagaaag tacccaagga gctgaaggga tctgcaaccc tatagatgga acaagaatat 163800 gaactaacca gtacccccag aggtcatatc tctagctgca tatgtagcag aagatggcct 163860 agtcggccat cagtgggaag agaggcccct aggtcttgca aactttatat gccccagtac 163920 aggggaacgc cagagtcaag aagtgggagt gggtgggtag gggatcaggg tcgggagagg 163980 gtatagggaa ctttcgggat agcatttgaa atgtaaataa agaaaacatc taataaaata 164040 gcaataaaga ataaccacta caaagagaga aaaggacaac aaaagcctgc agtaagtttg 164100 ctagctgaac agagcagatg aataaccggc agcagacttt gcaccacatc atttcctttg 164160 gccatccgca cagattgccg gaacaggcaa accagatctc catgcattct gttgtcccca 164220 ccctctgggc cataggactt ggacaaaggt aatgtcctgg atctgaaaga agttaagaca 164280 ctgggatggt atttaattaa ataataacag aggctcaact cggtgattgg cctggctgga 164340 ccagcctgat gtgttgataa ttgtctaatc ctctagtgga tatcaaaaaa aatcattgct 164400 ttcccaaggg aaggggacag ccaggagaag gagaggtata catggatgat aaacgttcca 164460 gatactggct aagtgcttac cagtaaattg aagtccacat ttcacagcat actccaggaa 164520 gtcatttctc cccagccaga ctcaacagtc tttgaaggct tctaaagtcc agctgggagg 164580 tagtgttgag cctctagcat gtctattcat agactactag atcccatgag agctgccatt 164640 acaattgctg gagtacattt tttcacttcc tatcacctaa ataaacttca cccactgtgt 164700 gggtttgcta atcccttcct catccttgct gtatccacca ccctgtcttg tttccttagc 164760 aactagacac tgctgagggc agggcagaag gctaaaaggg agcaccagta agtggaatga 164820 gtgagtggtg gcttggtggg ctgcagaggg gttagttctc acgttgcctt tttgagtatt 164880 ccccatcatt ttctcttcta gtgcgcaagt gcagggtcct cacatctatc cacactgtcc 164940 acacagagag aacacatttt taaaagctag cccaaagaga gaacaaagag ctgaggataa 165000 ctattcctaa cccaggatgt ctgccttaca ttacttttta ggtactttgc atttgtttgc 165060 tgctactatc attgtttgtt tactatattt taggagttcg aacaatccca ggcaccattc 165120 agacagaaag tggcaaatgt catctaaaag ggcagggctt aacattcccg tggcagtgtg 165180 tgcttcccca gtcagagaga gcactttctt gcttttactg ttttctaact cgagatttcc 165240 cccgcttttc tggagctttt ttttttttgt tgtttagctt ctcatcaaga aagtataagt 165300 tgtaggctgc gggagtgtct gtggggatgc gtttgttttg tttttgagac agagtttggc 165360 tatttagaga aggctagctt tgaactccca gagacccacc tgcttgtctc aagtgctggc 165420 actgaaagta tgtgtccaca tacctggcct cgtgtactaa ttttttgata attacaaaaa 165480 agggcaagaa aaaaagaaag aaagaaaatt tctttcttca gccattcatt ctgtagcctg 165540 ttgtgtccaa cactgatcta ggcattcttt tggggtactt gagcagaaag actagactgt 165600 gtactttcta gctgggctct tcaccagcag gtgcacattt cagtatttat ttggccactt 165660 gaaaatctca ctgttgattt tgtgataaaa aaaaaaaaca aaacccagga acttatttgt 165720 aaaacgatta ttcctggtgt ttatctgact caccttatca tctgtgttta aagattggtg 165780 gggtacactc tgagctattt gtacttggca agatccccaa ctctgagagc aaggttttag 165840 ctggctagtt ttcctccttc agcctctgcc aagtgcttgg tcctctagag aataatatgt 165900 accttttgtc cccacatatt aaatacaaat aaagagaaga acatgtcatc ttgaggcatt 165960 ggaagctatt aaggctatct caggcaatct ggacaactct acgccttggt cacatagtgg 166020 cttcttaata tgtaccatgg ctcactcaag aaaaatggag tggtgaccaa cttcagcctc 166080 tgagttttga gatggagact ctgagatagc tgtcaatatt ttgtaaaact gcttcctaac 166140 atcccgtacc cctttctgta tgtatatctg catgcacatg cacacatatg ggtatagagc 166200 ccgcactggc caaaagatga gagcagatct ccctggatcc agaattatat atggttgtga 166260 gcttccctat attgctgctg agagccaatc cagttctctg gaagagcaat actcactctc 166320 aactgctcag tcgcctctac aaaccaactg ttttttgttt gtttgtttgt ttgtttgttt 166380 gtctgtttgg tttggtttgg tttctcttag acagggtctc tgactgggac cgaggcttgc 166440 tgagtagggt aggctatttg accagcagcc ctcaggaatg caagtatttt cctcaacatc 166500 tgatttcctc tttcctttct ccctccctcc ctccctccct ccctccctcc ctccctccct 166560 cccttcctta atttcttcct ttcttctttc tttcttcctc cttccccctc tcttcccctc 166620 tctctctttc tttttttcca ttgccgacat gaagaagtag agatgagcat gaccaaggtc 166680 agtttctgct tttcctgtga caagggttct ccccagtcaa ccctggtctg cccacagaat 166740 tcttatccca ctgaaaggaa tagacatttc cctacatgaa gagttgtaaa gtggtggttg 166800 gtcccattgc atgcacagag agtgagagac gggtaacctg tgagacacct cctacataaa 166860 aagagttcca gtgatcttca aagtgtttgt cactcaccca gaaaaactcc agctcctcac 166920 taccaagcct aggaaaggaa gacagaaata cctagagagg aattccaatc acctcaagaa 166980 aaatggagta gtgaccagct tcagcctctg agttttgaga tggagactct gagagagctg 167040 tcaatatttt gtaaaaccgt ttcccactcg atagcattcc cagagtgaag gtttttctct 167100 cctcagccat gagctaggct tctggatttc tacatcctgt ttcatttgtg ttcttcctcc 167160 tatatttaag tgtaagcttg aatgagcgtc tcagttcact tcccacaata cgagaatttc 167220 ccaaacaagg agggcacacc aaactcatgt tagcagtgcc tcttcaggtg ttgcccattt 167280 ttctccttca aaagtcccca agcctcccag gtactacttt gccagttcag gttgttcagg 167340 ggaggtacac caagtgctga ttcccactga gctcctactg accctattca gtattgtgcc 167400 acagtttacc cttctcttct cttctcttct cttctcttct cttctcttct cttctcttct 167460 cttctcttct cttctcttct cttctcttct cttctctcct ctcctctcct ctcctctcct 167520 ctcctctcct ctcctctcct ctcctctcct ctcctctcct ctcctctcct ctcctctcct 167580 ttcccctccc ctcccctccc cctcccctcc cctcccctcc ccctcccctc ccctcccctc 167640 ccctcccctc ccctcccctc ccctcccctc ccctcccctc cttttctttt cttttctttt 167700 cttttctttt cttttctttt cttttctttt cttttctttt cttttctttt cttttctttg 167760 tgataggctt gggaagcttc ccctgcagga ctgtggagct cttgtgttca catttgtgcc 167820 ttttggaaag atgcttcagc ggctggaccc gaggcaagag tttgtcttca aaatgctgcc 167880 attgcctctg tcagttttca tctgcctgta aacttttttt ttttcttttt gccaaatctc 167940 ctgaagcaac ttgacctcaa ggtttttttt ttttaattcc ctcaactctt tctttattct 168000 ttgttccatt ttcactatct tttttttctt attagttaag agaaaccaaa gaattgattc 168060 acctgctcat tagttttctc gaatattccc ctgtgactaa agtaaaacta ggctacaccc 168120 ctaagagaaa gatgcctctt agggctcctg tcatcttcct attgtcttga ctggtgtgca 168180 tacaaagttt gaactatgaa actagggcat ggctgatttt ggctccagtg tttacttgtg 168240 aaccagtcta ttcactattg acctagcttt cagattccac agcccttcta gcttccctaa 168300 cataaaaaca taagcattat atgtatttag gaagtatcat gttgtgttcc aataatattt 168360 atgcagtttg acctcagttg tgcccttaat ccttgtagta cctgttttag ttagggtttt 168420 actgctgtga acaggcacca tgaccaagtc aactcataaa aacaacattt agtttgagct 168480 ggcttataaa ttcagaggtt cccaccaatg tcatcaaggt gggaacacga tagtgttcag 168540 gccgatatgg tgcaagagga gctgagagtt gtacattatc ataggaaggc tgctagcaga 168600 atagtgactt ccgggcagct aggatgaaga tcttaaagcc cacacccaca agagcacacc 168660 tactccaaca ggaccacacc ttctaatagt gccactccct gggccaagca tattcaaagc 168720 atcacagtat cttaattaga aaagtgggag ctatcccttg ttcatctgtc ttagttgtct 168780 tgactgctat aacaaaacac ctcaaactca ttcattcaca aag 168823 <210> 17 <211> 3294 <212> DNA <213> Artificial <220> <223> Synthetic <400> 17 atggggaccc gggcaagcag catcaccgcc ctcgcctctt gcagccgcac cgccggccaa 60 gtcggagcca cgatggtcgc cggctctctt ctcctgcttg gattcctcag caccatcaca 120 gcttggtaca ttctggtgcc aagtgtggcc attcccctgg ctatcgcctt cctcttcttc 180 ttcatctgtg tgtgccgcaa taaccagaag tcttcatcag gtaccgattt aaatgatcca 240 gtggtcctgc agaggagaga ttgggagaat cccggtgtga cacagctgaa cagactagcc 300 gcccaccctc cctttgcttc ttggagaaac agtgaggaag ctaggacaga cagaccaagc 360 cagcaactca gatctttgaa cggggagtgg agatttgcct ggtttccggc accagaagcg 420 gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca 480 aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta tcccattacg 540 gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat 600 gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg 660 gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg 720 tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg 780 ctgcgctgga gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt 840 ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc 900 actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc 960 gagttgcgtg actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc 1020 agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc 1080 gtcacactac gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc 1140 tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc 1200 gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg 1260 ttgctgattc gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat 1320 gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc 1380 tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat 1440 gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat 1500 gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt 1560 aatcacccga gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac 1620 gacgcgctgt atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc 1680 ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa 1740 gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga 1800 gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt 1860 ttcgctaaat actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg 1920 gactgggtgg atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac 1980 ggcggtgatt ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt 2040 gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag 2100 ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat 2160 aacgagctcc tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg 2220 cctctggatg tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg 2280 gagagcgccg ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg 2340 tcagaagccg ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg 2400 acgctccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc 2460 atcgagctgg gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg 2520 tggattggcg ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg 2580 ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa 2640 cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat 2700 acacttgctg atgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc 2760 ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt 2820 gatgttgaag tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg 2880 gcgcaggtag cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac 2940 cgccttactg ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg 3000 tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca 3060 caccagtggc gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg 3120 gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt 3180 ttccatatgg ggattggtgg cgacgactcc tggagcccgt cagtatcggc ggaattccag 3240 ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaataata ataa 3294

Claims (46)

  1. DR6 단백질의 세포질 도메인 전체를 인코딩하는 제1 뉴클레오티드 서열이 결여된 변형 내인성 DR6 유전자좌를 게놈 내에 포함하는 유전자 조작 설치류.
  2. 제1항에 있어서, 상기 변형 내인성 DR6 유전자좌는 성숙 DR6 단백질 전체를 인코딩하는 제2 뉴클레오티드 서열이 추가로 결여된 유전자 조작 설치류.
  3. 제1항 또는 제2항에 있어서, 상기 변형 내인성 DR6 유전자좌는 막 관통 도메인 및 리포터 단백질 중 적어도 하나에 작동가능하게 융합된 기능성 DR6 신호 펩티드를 인코딩하는 핵산 서열을 포함하는 유전자 조작 설치류.
  4. 제3항에 있어서, 상기 핵산 서열은 상기 막 관통 도메인에 작동가능하게 융합된 상기 기능성 DR6 신호 펩티드를 인코딩하고, 상기 막 관통은 상기 리포터 단백질에 작동가능하게 융합된 유전자 변형 설치류.
  5. 제3항 또는 제4항에 있어서, 상기 기능성 DR6 신호 펩티드는 내인성 DR6 신호 펩티드인 유전자 변형 설치류.
  6. 제3항 내지 제5항 중 어느 한 항에 있어서, 상기 막 관통 도메인은 내인성 DR6 막 관통 도메인이 아닌 유전자 변형 설치류.
  7. 제3항 내지 제6항 중 어느 한 항에 있어서, 상기 막 관통 도메인은 ROR1 막 관통 도메인인 유전자 변형 설치류.
  8. 제3항 내지 제7항 중 어느 한 항에 있어서, 상기 리포터 단백질은 β 갈락토시다제인 유전자 변형 설치류.
  9. 제3항 내지 제8항 중 어느 한 항에 있어서, 상기 핵산 서열은 내인성 DR6 전사 조절 서열에 작동가능하게 연결된 유전자 변형 설치류.
  10. 제1항 내지 제9항 중 어느 한 항에 있어서, 상기 유전자 변형 설치류는 변형 내인성 DR6 유전자좌에서 이형접합성인 유전자 변형 설치류.
  11. 제1항 내지 제10항 중 어느 한 항에 있어서, 상기 설치류는 운동 뉴런 기능 장애를 나타내는 유전자 변형 설치류.
  12. 제11항에 있어서, 상기 운동 뉴런은 상위 운동 뉴런 또는 하위 운동 뉴런인 유전자 변형 설치류.
  13. 제12항에 있어서, 상기 운동 뉴런 기능 장애는 상기 설치류가 4주령이 초과할 때까지 블라인드 주관적 신경학적 점수화 검정 (blinded subjective neurological scoring assay), 로타로드 테스트(rotarod test), 캣워크 테스트(catwalk test), 오픈 필드 테스트(open field test), 및 중량 측정에서 분명하지 않은 유전자 변형 설치류.
  14. 제1항 내지 제13항 중 어느 한 항에 있어서, 상기 설치류는 대조군 설치류와 비교하여 통각수용의 저하를 나타내는 유전자 변형 설치류.
  15. 제1항에 있어서, 상기 설치류는 래트 또는 마우스인 유전자 변형 설치류.
  16. 제15항에 있어서, 상기 설치류는 성숙 DR6 단백질 전체를 인코딩하는 제1 뉴클레오티드 서열이 결여된 변형 내인성 DR6 유전자좌를 포함하는 마우스이며,
    상기 내인성 마우스 DR6 유전자좌는, 적어도 하나의 내인성 DR6 전사 조절 서열에 작동가능하게 연결된, β-갈락토시다제에 작동가능하게 융합된 ROR1 막 관통 도메인에 작동가능하게 융합된 내인성 DR6 신호 펩티드를 인코딩하는 핵산 서열을 포함하는 유전자 변형 설치류.
  17. 제16항에 있어서, 상기 마우스는 129 계통, C57BL/6 계통, 및 혼합된 C57BL/6 x 129 계통으로 이루어진 군으로부터 선택된 계통이고, 상기 마우스는 8주령 내지 20주령의 대조군 야생형 동물과 비교하여 중량 증가의 감소를 나타내는 유전자 변형 설치류.
  18. 제1항 내지 제17항 중 어느 한 항의 유전자 변형 설치류로부터 유래된 세포 또는 조직.
  19. 제18항에 있어서, 상기 세포는 운동 뉴런 또는 배아 줄기 세포인 세포 또는 조직.
  20. DR6 세포질 도메인 전체를 인코딩하는 제1 내인성 뉴클레오티드 서열을 상기 설치류의 내인성 DR6 유전자좌에서 결실시키는 단계(들)를 포함하는 제1항에 따른 유전자 변형 설치류의 제조 방법.
  21. 제20항에 있어서, 상기 결실 단계는 전장의 내인성 성숙 DR6 단백질을 인코딩하는 제2 내인성 뉴클레오티드 서열을 막 관통 도메인 및/또는 리포터 유전자를 인코딩하는 핵산 서열로 대체하는 단계를 포함하고, 상기 핵산은 내인성 DR6 전사 조절 및 신호 펩티드 서열과 작동가능하게 연결된 방법.
  22. 대조군 운동 뉴런 세포와 비교하여 증가된 산화 스트레스를 나타내는 운동 뉴런 집단의 제조 방법으로서, 상기 방법은 제1항 내지 제17항 중 어느 한 항에 따른 설치류에서 단리된 내세포집단 배아 줄기 세포 (inner mass embryonic stem cell)로부터 배상체 (embryoid body)를 수립하는 단계 및 상기 배상체를 운동 뉴런 집단으로 분화시키는 단계를 포함하는 방법.
  23. 운동 뉴런 기능 장애를 조절하기 위한 후보 제제의 스크리닝 방법으로서,
    (a) DR6 세포질 도메인 전체를 인코딩하는 제1 뉴클레오티드 서열이 결여된 변형 내인성 DR6 유전자좌를 게놈 내에 포함하며 운동 뉴런 기능 장애를 발현하는 설치류에 후보 제제를 투여하는 단계; 및
    (b) 상기 설치류에서, 시험 대조군 설치류와 비교하여, 상기 운동 뉴런 기능 장애의 하나 이상의 증상에 대한 상기 후보 제제의 임의의 조절 효과를 결정하는 단계를 포함하며,
    상기 설치류에서, 상기 시험 대조군 설치류와 비교하여, 상기 운동 뉴런 기능 장애의 적어도 하나의 증상에 대한 조절 효과의 존재는 상기 후보 제제가 운동 뉴런 기능 장애를 조절하는데 유용하다는 것을 나타내는 방법.
  24. 제23항에 있어서, 상기 변형 내인성 DR6 유전자좌는 성숙 내인성 DR6 단백질 전체를 인코딩하는 제2 뉴클레오티드 서열이 추가로 결여된 방법.
  25. 제23항 또는 제24항에 있어서, 상기 변형 내인성 DR6 유전자좌는 막 관통 도메인 및 리포터 단백질 중 적어도 하나에 작동가능하게 융합된 기능성 DR6 신호 펩티드를 인코딩하는 핵산 서열을 포함하는 방법.
  26. 제23항 내지 제25항 중 어느 한 항에 있어서, 상기 제제는 블라인드 주관적 ALS-TDI 신경학적 점수화, 로타로드 테스트, 캣워크 테스트, 오픈 필드 테스트, 중량 측정 또는 통증 자극에 대한 반응 지체 시간의 결정에 의한 상기 증상의 검출 전에 투여되는 방법.
  27. 제23항 내지 제26항 중 어느 한 항에 있어서, 상기 제제는 블라인드 주관적 ALS-TDI 신경학적 점수화, 로타로드 테스트, 캣워크 테스트, 오픈 필드 테스트, 중량 측정 또는 통증 자극에 대한 반응 지체 시간의 결정 중 하나 이상에 의한 상기 증상의 검출 후에 투여되는 방법.
  28. 제23항 내지 제27항 중 어느 한 항에 있어서, 상기 제제는 둘 이상의 상이한 시점에 투여되는 방법.
  29. 제23항 내지 제28항 중 어느 한 항에 있어서, 상기 적어도 하나의 증상은, 상기 설치류와 계통이 동일하나 게놈 내에 상기 핵산을 포함하지 않는 대조군 설치류와 비교하여, 척추후만증, 비정상적인 뒷다리 움켜쥐기, 운동 협응 결손, 운동 학습 능력 결손, 중량 감소 및 감각 지각 결손으로부터 선택되는 방법.
  30. 제23항 내지 제29항 중 어느 한 항에 있어서, 상기 적어도 하나의 증상은 상위 운동 뉴런 기능 장애로 인한 것인 방법.
  31. 제30항에 있어서, 상기 적어도 하나의 증상은 진전, 경직성 마비 (강직), 비정상적인 반사 및 이들의 조합으로 이루어진 군으로부터 선택되는 방법.
  32. 제23항 내지 제29항 중 어느 한 항에 있어서, 상기 적어도 하나의 증상은 하위 운동 뉴런 기능 장애로 인한 것인 방법.
  33. 제32항에 있어서, 상기 적어도 하나의 증상은 근육 약화 및 소모, 근섬유다발수축 및 이들의 조합으로 이루어진 군으로부터 선택되는 방법.
  34. 제23항 내지 제33항 중 어느 한 항에 있어서, 운동 뉴런 기능 장애의 상기 적어도 하나의 증상은 중량 증가의 감소인 방법.
  35. 제23항 내지 제34항 중 어느 한 항에 있어서, 운동 뉴런 기능 장애의 상기 적어도 하나의 증상은 통각수용의 저하인 방법.
  36. 제23항 내지 제35항 중 어느 한 항에 있어서, 상기 설치류는 성숙 DR6 단백질 전체를 인코딩하는 제1 뉴클레오티드 서열이 결여된 변형 내인성 DR6 유전자좌를 포함하는 마우스이며,
    상기 내인성 마우스 DR6 유전자좌는, 적어도 하나의 내인성 DR6 전사 조절 서열에 작동가능하게 연결된, β-갈락토시다제에 작동가능하게 융합된 ROR1 막 관통 도메인에 작동가능하게 융합된 내인성 DR6 신호 펩티드를 인코딩하는 핵산 서열을 포함하는 방법.
  37. 제36항에 있어서, 상기 마우스는 129 계통, C57BL/6 계통, 및 혼합된 C57BL/6 x 129 계통으로 이루어진 군으로부터 선택된 계통인 방법.
  38. 운동 뉴런에서 산화 스트레스를 감소시키기 위한 후보 제제의 스크리닝 방법으로서,
    (a) 제1항에 따른 설치류로부터 유래된 운동 뉴런을 제제의 존재 또는 부재하에 배양하는 단계; 및
    (b) 상기 제제가 상기 운동 뉴런에서, 상기 설치류로부터 유래되나 상기 제제의 부재하에 배양된 대조군 운동 뉴런과 비교하여, 산화 스트레스를 억제, 저해 및/또는 감소시키는지의 여부를 결정하는 단계를 포함하며, 상기 운동 뉴런에서의 산화 스트레스의 억제, 저해 및/또는 감소는 운동 뉴런에서 산화 스트레스를 감소시키기 위한 후보 제제를 나타내는 방법.
  39. 작동가능한 연결로 그리고 5'에서 3' 방향으로, DR6 신호 펩티드를 인코딩하는 제1 핵산 서열, 막 관통 도메인을 인코딩하는 제2 핵산, 리포터 유전자를 인코딩하는 제3 핵산, 및/또는 약물 선택 카세트를 인코딩하는 제4 핵산을 포함하는 핵산.
  40. 제39항에 있어서, 상기 제1 핵산 서열은 서열 번호 6으로 제시되고, 상기 제2 핵산 서열은 서열 번호 7로 제시되고, 상기 제3 핵산 서열은 서열 번호 8로 제시되고/되거나, 상기 제4 핵산은 서열 번호 3으로 제시되는 핵산 서열.
  41. 제39항 또는 제40항에 있어서, 제1 표적화 아암 및 제2 표적화 아암을 추가로 포함하며, 상기 제1 표적화 아암은 상기 제1, 제2 및/또는 제3 핵산 서열의 5'에 위치하고 그러한 핵산 서열에 작동가능하게 연결되며, 상기 제2 표적화 아암은 상기 제1, 제2 및/또는 제3 핵산 서열의 3'에 위치하고 그러한 핵산 서열에 작동가능하게 연결되며, 상기 제1 표적화 아암 및 상기 제2 표적화 아암은 적어도 하나의 내인성 비-인간 동물 조절 요소에 작동가능하게 연결된 내인성 비-인간 동물 DR6 유전자좌 내에 삽입하기 위한 핵산의 표적화를 위한 것인 핵산.
  42. 제41항에 있어서, 상기 비-인간 동물은 설치류인 핵산.
  43. 제42항에 있어서, 상기 설치류는 마우스인 핵산 서열.
  44. 제43항에 있어서, 상기 핵산은 서열 번호 5 또는 서열 번호 17로 제시되는 서열을 포함하는 핵산 서열.
  45. 제39항 내지 제44항 중 어느 한 항의 핵산을 포함하는 설치류 세포.
  46. 제45항에 있어서, 상기 설치류 세포는 배아 줄기 세포인 설치류 세포.
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