KR20220104764A - 신경계 세포의 동결 방법 - Google Patents
신경계 세포의 동결 방법 Download PDFInfo
- Publication number
- KR20220104764A KR20220104764A KR1020227020620A KR20227020620A KR20220104764A KR 20220104764 A KR20220104764 A KR 20220104764A KR 1020227020620 A KR1020227020620 A KR 1020227020620A KR 20227020620 A KR20227020620 A KR 20227020620A KR 20220104764 A KR20220104764 A KR 20220104764A
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- South Korea
- Prior art keywords
- cells
- cell
- cell aggregate
- positive
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
- A61P25/16—Anti-Parkinson drugs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
- A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
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- A01N1/0221—
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- A01N1/0284—
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
- A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/16—Physical preservation processes
- A01N1/162—Temperature processes, e.g. following predefined temperature changes over time
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
- A61L27/3675—Nerve tissue, e.g. brain, spinal cord, nerves, dura mater
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/383—Nerve cells, e.g. dendritic cells, Schwann cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3834—Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
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- C—CHEMISTRY; METALLURGY
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0619—Neurons
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
- C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases [EC 2.]
- C12N2501/727—Kinases (EC 2.7.)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
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- C12N2513/00—3D culture
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- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/52—Fibronectin; Laminin
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Neurology (AREA)
- Neurosurgery (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Virology (AREA)
- Epidemiology (AREA)
- Developmental Biology & Embryology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Dermatology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Immunology (AREA)
- Psychology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Vascular Medicine (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JPJP-P-2019-209929 | 2019-11-20 | ||
| JP2019209929 | 2019-11-20 | ||
| PCT/JP2020/043275 WO2021100829A1 (ja) | 2019-11-20 | 2020-11-19 | 神経系細胞の凍結方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20220104764A true KR20220104764A (ko) | 2022-07-26 |
Family
ID=75980133
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020227020620A Pending KR20220104764A (ko) | 2019-11-20 | 2020-11-19 | 신경계 세포의 동결 방법 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20230000071A1 (https=) |
| EP (1) | EP4063496A4 (https=) |
| JP (3) | JP7491533B2 (https=) |
| KR (1) | KR20220104764A (https=) |
| CN (1) | CN115175989A (https=) |
| AU (1) | AU2020387259A1 (https=) |
| CA (1) | CA3162268A1 (https=) |
| IL (1) | IL293137A (https=) |
| MY (1) | MY209020A (https=) |
| WO (1) | WO2021100829A1 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2024293098A1 (en) * | 2023-07-14 | 2026-02-12 | Advanced Biologics, Llc | Increasing viability of cells within a frozen cellular implant |
| JP7840913B2 (ja) * | 2023-09-15 | 2026-04-06 | 株式会社クラレ | 細胞凝集体および細胞凝集体の製造方法 |
| US20250157580A1 (en) * | 2023-11-13 | 2025-05-15 | Aspen Neuroscience, Inc. | Methods of predicting dopamine production capability of differentiated neuronal cells |
| JP2025094837A (ja) * | 2023-12-13 | 2025-06-25 | 国立大学法人京都大学 | 凍結細胞の製造方法 |
| WO2025166041A1 (en) * | 2024-01-31 | 2025-08-07 | BrainXell Therapeutics, Inc. | Methods for preserving neural progenitor cell survival in vitro and in vivo |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008501320A (ja) | 2004-06-02 | 2008-01-24 | イーエス・セル・インターナショナル・プライヴェート・リミテッド | 細胞保存方法 |
| JP2011103885A (ja) | 1999-09-24 | 2011-06-02 | Abt Holding Co | 多能性胚様幹細胞、その組成物、方法および使用 |
| JP2013110988A (ja) | 2011-11-25 | 2013-06-10 | Sumitomo Chemical Co Ltd | 多能性幹細胞由来の組織の凍結保存方法 |
| JP2015521469A (ja) | 2012-06-11 | 2015-07-30 | ロンザ ウォカーズビル インコーポレーティッド | ヒト人工多能性幹細胞からの運動ニューロン前駆細胞(mnp)のインビトロ分化法およびmnpの凍結保存法 |
| JP2017104061A (ja) | 2015-12-10 | 2017-06-15 | 学校法人慶應義塾 | ヒトiPS細胞由来神経幹細胞/前駆細胞の凍結方法 |
| WO2017159862A1 (ja) | 2016-03-18 | 2017-09-21 | 国立大学法人京都大学 | 多能性幹細胞由来心筋細胞の凝集体の凍結方法 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993007745A1 (en) * | 1991-10-23 | 1993-04-29 | Cellpro, Incorporated | Method for freezing engrafting cells |
| US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| US6280718B1 (en) | 1999-11-08 | 2001-08-28 | Wisconsin Alumni Reasearch Foundation | Hematopoietic differentiation of human pluripotent embryonic stem cells |
| WO2002101057A1 (fr) | 2001-06-08 | 2002-12-19 | Dnavec Research Inc. | Transfert genique dans des cellules souches embryonnaires de primate a l'aide d'un virus de l'immunodeficience simienne de pseudo type vsv-g utilise comme vecteur |
| US7811819B2 (en) * | 2005-05-31 | 2010-10-12 | The University Of Washington | Cryopreservation of primate embryonic stem cells |
| JPWO2009148170A1 (ja) | 2008-06-06 | 2011-11-04 | 独立行政法人理化学研究所 | 幹細胞の培養方法 |
| JP6210881B2 (ja) | 2011-10-31 | 2017-10-11 | 国立研究開発法人理化学研究所 | 幹細胞の培養方法 |
| AU2014316100B2 (en) | 2013-09-05 | 2020-11-05 | Eisai R&D Management Co., Ltd. | New method for inducing dopamine-producing neural precursor cells |
| ES2732730T3 (es) | 2013-11-22 | 2019-11-25 | Riken | Método para producir telencéfalo o tejido progenitor del mismo |
| CA2956130C (en) | 2014-07-25 | 2023-09-26 | Riken | Method for producing adenohypophysis or precursor tissue thereof |
| US10781420B2 (en) | 2014-09-08 | 2020-09-22 | Riken | Method for producing cerebellar progenitor tissue |
| LU92845B1 (en) * | 2015-10-08 | 2017-05-02 | Univ Du Luxembourg Campus Belval | Means and methods for generating midbrain organoids |
| CN108495929B (zh) | 2016-01-22 | 2023-04-18 | 国立大学法人名古屋大学 | 由人多能干细胞向下丘脑神经元的分化诱导 |
| CN105850980B (zh) * | 2016-04-14 | 2018-06-12 | 广州赛莱拉干细胞科技股份有限公司 | 一种角膜缘干细胞的冻存液及冻存方法 |
| JP7011260B2 (ja) * | 2016-04-22 | 2022-02-10 | 国立大学法人京都大学 | ドーパミン産生神経前駆細胞の製造方法 |
| US20200405768A1 (en) * | 2018-02-19 | 2020-12-31 | Sumitomo Dainippon Pharma Co., Ltd. | Cell Aggregate, Mixture of Cell Aggregates, and Method for Preparing Same |
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2020
- 2020-11-19 AU AU2020387259A patent/AU2020387259A1/en active Pending
- 2020-11-19 CN CN202080093441.4A patent/CN115175989A/zh active Pending
- 2020-11-19 WO PCT/JP2020/043275 patent/WO2021100829A1/ja not_active Ceased
- 2020-11-19 JP JP2021558457A patent/JP7491533B2/ja active Active
- 2020-11-19 EP EP20890883.0A patent/EP4063496A4/en active Pending
- 2020-11-19 MY MYPI2022002608A patent/MY209020A/en unknown
- 2020-11-19 US US17/778,250 patent/US20230000071A1/en active Pending
- 2020-11-19 CA CA3162268A patent/CA3162268A1/en active Pending
- 2020-11-19 KR KR1020227020620A patent/KR20220104764A/ko active Pending
- 2020-11-19 IL IL293137A patent/IL293137A/en unknown
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2024
- 2024-05-08 JP JP2024075610A patent/JP7787527B2/ja active Active
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2025
- 2025-11-27 JP JP2025205398A patent/JP2026026215A/ja active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| JP7787527B2 (ja) | 2025-12-17 |
| US20230000071A1 (en) | 2023-01-05 |
| JP7491533B2 (ja) | 2024-05-28 |
| EP4063496A4 (en) | 2024-07-03 |
| MY209020A (en) | 2025-06-17 |
| AU2020387259A1 (en) | 2022-06-09 |
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| CA3162268A1 (en) | 2021-05-25 |
| JPWO2021100829A1 (https=) | 2021-05-27 |
| CN115175989A (zh) | 2022-10-11 |
| IL293137A (en) | 2022-07-01 |
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