KR20220090610A - A method of extracting compsition from seaweed - Google Patents

Abstract

The present invention is for extracting various physiologically active ingredients and nutrients contained in seaweed together, and the extraction method of the present invention comprises the steps of freezing the pulverized seaweed so as to destroy the cells of the wet pulverized seaweed; mixing the pulverized seaweed with a predetermined amount of water to create a mixture; adding sodium bicarbonate to the mixture so that the pH of the mixture reaches a preset range; adding a first degrading enzyme for decomposing the alginic acid to the mixture so that the average molecular weight of the alginic acid in the mixture is equal to or less than a reference value; and adding a second degrading enzyme for degrading proteins in the mixture to the mixture.

Description

Method of extracting complex composition from seaweed {A METHOD OF EXTRACTING COMPSITION FROM SEAWEED}

The present invention relates to a method for extracting a complex composition from seaweed, and more particularly, to extracting a complex composition comprising fucoidan, a viscous polysaccharide, alginic acid, amino acids, minerals, vitamins, fucosterol, fucoxanthin and beta-carotene from seaweed. it's about how

In general, seaweed generates and releases spores from leaves, whereas seaweed sporangia is almost the only one in brown seaweeds that has a separate sporangium. The spore leaves, also called seaweed ears, are wrinkled and hang on the root of seaweed. The sporophyte is the part that corresponds to the seed bag of a plant, and just as the seeds of all plants are nutrient mass, the seaweed sporophyte is a region where germ cell colonists are condensed compared to general seaweed, and contains a lot of physiologically active substances such as fucoidan and alginic acid.

Fucoidan, which is present in an amount of 18 to 23% (w/w) of seaweed spore leaves, is in the spotlight for its immune enhancement, apoptotic function to kill cancer cells, metastasis suppression, alleviation of side effects of chemotherapy, anti-allergy, antiviral, and antibacterial action, etc. It is a bioactive ingredient received.

Alginic acid is also a kind of viscous polysaccharide contained in seaweed. It is used as an industrial stabilizer, an additive to increase the viscosity of foods such as ice cream/pudding/gel, surgical thread, adhesive raw material, cosmetic additive, etc. The demand for it is increasing. . In addition, fucoxanthin, a carotenoid contained in seaweed sporophyte, has been shown to have an anti-obesity effect by decomposing white adipose tissue.

Until now, it was a method of extracting only a single component necessary to utilize these functional components. For example, during the manufacturing process of fucoidan, only 3 to 5% of the 18 to 23% fucoidan component contained in seaweed spore leaves is extracted, and the remaining seaweed spore leaves are discarded without being used for other purposes. low utilization.

SUMMARY OF THE INVENTION The present invention is to solve the problems of the prior art, and an object of the present invention is to provide a method for extracting various physiologically active ingredients and nutrients contained in seaweed together.

Another object of the present invention is to provide a method for efficiently extracting physiologically active ingredients and nutrients from seaweeds.

According to an aspect of the present invention, there is provided a method for extracting a composite composition comprising the steps of: freezing the pulverized seaweed so as to destroy the cells of the wet pulverized seaweed; mixing the pulverized seaweed with a predetermined amount of water to create a mixture; adding sodium bicarbonate to the mixture so that the pH of the mixture reaches a preset range; adding a first degrading enzyme for decomposing the alginic acid to the mixture so that the average molecular weight of the alginic acid in the mixture is equal to or less than a reference value; and adding a second degrading enzyme for degrading proteins in the mixture to the mixture.

In the step of adding sodium bicarbonate to the mixture so that the pH of the mixture reaches within a preset range, sodium hydrogen carbonate is added to the mixture so that the pH of the mixture reaches 5 to 7.

The first degrading enzyme is a gamma proteobacterial enzyme, and the first degrading enzyme corresponding to 0.05 to 0.1 wt% of seaweed is added to the mixture. In addition, the first degrading enzyme is preferably added to the mixture so that the average molecular weight of alginic acid in the mixture is 40,000 daltons or less.

After the first degrading enzyme is added to the mixture, the second degrading enzyme is added to the mixture after a certain period of time, wherein the second degrading enzyme is alcalase and flavorzyme 1: 1, and the second degrading enzyme corresponding to 0.1 to 0.2 wt% of seaweed is added to the mixture.

After adding the second degrading enzyme to the mixture, heating at a temperature of 90° C. or higher for 1 to 3 hours to stop protein degradation, fucoidan, alginic acid, amino acids, minerals, vitamins, fucosterol and beta-carotene It further comprises the step of extracting the composite composition comprising the mixture.

Since the complex composition extraction method according to the present invention can extract various physiologically active ingredients and nutrients contained in seaweed together, various physiologically active ingredients and nutritional ingredients are extracted using fewer raw materials than in the prior art of extracting individually. can be produced, and thus the production cost can be significantly reduced.

In addition, it is possible to increase the yield of physiologically active ingredients and nutrients by removing factors that interfere in the process of decomposing proteins in seaweed, thereby increasing production efficiency.

It should be understood that the effects of the present invention are not limited to the above-described effects, and include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

1 is a flowchart illustrating a method for extracting a complex composition from seaweed according to an embodiment of the present invention.

Hereinafter, the embodiments disclosed in the present specification will be described in detail with reference to the accompanying drawings, but the same or similar components are assigned the same reference numbers regardless of reference numerals, and redundant description thereof will be omitted. In addition, in describing the embodiments disclosed in the present specification, if it is determined that detailed descriptions of related known technologies may obscure the gist of the embodiments disclosed in this specification, the detailed description thereof will be omitted. In addition, the accompanying drawings are only for easy understanding of the embodiments disclosed in the present specification, and the technical idea disclosed herein is not limited by the accompanying drawings, and all changes included in the spirit and scope of the present invention , should be understood to include equivalents or substitutes.

Hereinafter, a method for extracting a composite composition according to the present invention will be described in detail with reference to the accompanying drawings.

1 is a flowchart illustrating a method for extracting a composite composition according to an embodiment of the present invention. Since the complex composition extraction method shown in FIG. 1 is performed by an automated manufacturing apparatus, it is preferable that all processes included in the extraction method of the present invention be interpreted as being performed by the manufacturing apparatus.

First, the seaweed is washed to remove residues and impurities from the tidal flat, the seaweed is immersed in water to remove the base, and then the seaweed is wet pulverized (S11). The reason for wet pulverizing seaweed is so that moisture is absorbed into the pulverized seaweed so that the cells of the seaweed are well destroyed in the subsequent freezing process. Here, the seaweed may include at least one of brown algae such as seaweed, kelp, mother-of-pearl, seaweed, halibut, mozuku, bladder wrack, giant kelp, etc. It is more preferable to use only raw materials

Next, the process of destroying the cells of the wet pulverized seaweed is performed, and for cell destruction, the cells are frozen at a temperature of -50° C. or less until the seaweed is completely frozen (S12). The freezing time of seaweed varies depending on the amount of pulverized seaweed. For example, 100 kg of pulverized seaweed is preferably frozen for about 10 hours or more. The cell wall is not easy to decompose because polysaccharides such as cellulose, alginate, and fucoidan are tightly bound to each other. This is to easily release active ingredients found in the intercellular space, especially germ cells, and to increase the yield.

In addition, as another method of destroying the cells of seaweed, cell membrane degrading enzyme tunicase may be added to the pulverized seaweed to destroy the cells. In this case, if the pulverized seaweed and the cell membrane decomposing enzyme are mixed and stored in a high-pressure container, the cells can be destroyed more effectively. It is also possible to destroy the cells of the crushed seaweed first by using a cell membrane degrading enzyme and then freeze the seaweed to destroy the cells secondarily. It is also possible to destroy cells secondary.

Thereafter, a predetermined amount of water is mixed with the cell-destroyed seaweed to produce a mixture (S13). For example, 4 to 6 times the amount of purified water before grinding the seaweed is mixed with the pulverized seaweed.

And sodium bicarbonate (NaHCO ₃ ) is added to the mixture so that the pH of the hydrogen ion index of the mixture reaches within a preset range (S14). At this time, it is preferable to add a certain amount of sodium hydrogen carbonate while maintaining the mixture at a temperature of 50° C. until the pH of the mixture reaches 5 to 7. When the pH of the mixture is 7, it is more preferable to add sodium bicarbonate until the pH of the mixture is 7 while checking the pH value of the mixture (S15) because it is easy in the subsequent proteolysis process.

When the pH of the mixture reaches a preset range, a first degrading enzyme for decomposing alginic acid in the mixture is added to the mixture (S16). Here, the first degrading enzyme lowers the molecular weight of alginic acid so that the average molecular weight of alginic acid, which was 200,000 to 600,000 daltons, is 40,000 daltons or less, which hydrolyzes alginic acid in the mixture to lower the viscosity of the mixture. Alginic acid may cause difficulties in the solubility of protein molecules due to its high viscosity and anionic cell wall polysaccharide, which may affect the extraction of algae protein, lowering the viscosity of the mixture, and subsequently decomposing proteins in the mixture. Make sure that the mucilage of alginic acid does not interfere with protein breakdown. As the first degrading enzyme for decomposing alginic acid, a Gammaproteobacteria enzyme may be used, and it is preferable to add the first degrading enzyme corresponding to 0.05 to 0.1% of the weight of seaweed before grinding to the mixture.

And the mixture and the first degrading enzyme are stirred, but it is preferable to stir for 1 to 5 hours so that the average molecular weight of alginic acid can fall below a certain level. Since it is very important to lower the viscosity of alginic acid, it is more preferable to determine whether the average molecular weight of alginic acid is lowered to a certain level or less and determine the stirring time accordingly.

When it is determined that the average molecular weight of alginic acid has fallen below the reference value (40Kda), a second degrading enzyme for decomposing proteins in the mixture is added to the mixture (S17). As the second degrading enzyme, an enzyme in which alcalase and Flavorzyme are mixed in a 1:1 ratio or Bacillus alcalophilus may be used, and the weight of seaweed before grinding It is preferable to add the second degrading enzyme corresponding to 0.1 to 0.2% of the mixture. And it is preferable to stir the mixture and the second degrading enzyme for a predetermined time. At this time, the protein is decomposed into peptides and amino acids.

Then, the mixture is heated at a temperature of 90° C. or higher for 1 to 3 hours to stop protein degradation (S18).

Then, a complex composition containing fucoidan, alginic acid, minerals (zinc, potassium, calcium, magnesium, etc.), hydrolyzed amino acids, vitamins, chlorophyll, fucosterol and beta-carotene, etc. is extracted from the mixture (S19), at this time, after separating the mixture into a solid and a liquid using a filter press, the separated liquid can be freeze-dried to obtain a composite composition.

The amino acid contained in the complex composition is a hydrolyzed component from the protein contained in the seaweed. The absorption rate is higher through ingestion of hydrolyzed amino acids than amino acids absorbed by the body through protein intake. Hydrolyzed amino acids are absorbed in the small intestine and participate in all cell signaling processes through the blood, so they bind to cell signaling molecules as intracellular receptors and undergo biochemical reactions. Therefore, since the hydrolyzed amino acid can help the physiological activity mechanism of fucoidan, when fucoidan is ingested together with the hydrolyzed amino acid, the effect of increasing the physiological activity function can be obtained. In addition, hydrolyzed amino acids generate energy as raw materials for muscles in the body, act as a catalyst for metabolism, and help the regeneration and recovery of human tissues.

Alginic acid hydrolyzes alginic acid to lower the viscosity because its viscous component lowers the proteolytic enzyme, so it not only helps to decompose proteins well, but also because the alginic acid in the complex composition is low molecular weight at the level of oligosaccharides (40Kda). It can further enhance the physiologically active functions such as proliferation of probiotics, anti-obesity, and cholesterol reduction.

In addition, the minerals in the composite composition have a higher content than minerals contained in other foods and are very similar to the constituents of the human body.

The complex composition contains useful substances such as unsaturated fatty acid EPA, bioactive ingredients fucosterol, fucoxanthin, and beta-carotene/beta-glucan, which are known to have a significant effect on biological activity and changes in intestinal microflora.

The composite composition of the present invention as described above can be used in functional supplements, general foods, food additives, beverages, animal feed, and the like.

Example One

After washing with seawater and immersing in purified water to wet-pulverize 100 kg of fresh seaweed, the base was controlled, and then rapidly frozen at a temperature of -70° C. or lower for 10 hours to destroy the cells of the pulverized seaweed.

After that, the cell-destroyed seaweed and 4 times water (400 kg) of purified water are put in an extractor with a capacity of 1 ton and mixed, and while the mixture is maintained at a temperature of 50 ° C, a certain amount of sodium bicarbonate is added until the pH of the mixture is 7. were added each.

When the pH of the mixture reaches 7, 50 g of an enzyme obtained from gamma proteobacteria (Accession No. SD1006), an alginic acid degrading enzyme, is added to the mixture to decompose alginic acid in the mixture, and the average molecular weight of alginic acid in the mixture is 40,000 The mixture was stirred until it was below daltons.

One hour after the alginate lyase was added to the mixture, 100 g of protease was added to the mixture to decompose proteins in the mixture. Here, as the proteolytic enzyme, a 1:1 mixture of alcalase and flavorzyme was used. Then, the mixture to which the protease was added was reacted with stirring for 2 hours.

Then, in order to stop the protein degradation, the mixture was heated at a temperature of 90° C. or higher for 2 hours and then allowed to stand.

Thereafter, the mixture was separated into a solid and a liquid using a filter press, and the separated liquid was freeze-dried to obtain a 35 kg composite composition.

Example 2

After washing with seawater and immersing in purified water to control the base, 100 kg of dried seaweed was wet-milled, and then frozen at a temperature of -70° C. or lower for 10 hours to destroy the cells of the pulverized seaweed.

Then, the cell-destroyed seaweed and 6-fold water (600 kg) of purified water were added to the extractor and mixed, and while the mixture was maintained at a temperature of 50° C., sodium bicarbonate was added in a certain amount until the pH of the mixture reached 7.

When the pH of the mixture reaches 7, 100 g of gamma proteobacterial enzyme, an alginate degrading enzyme, is added to the mixture to decompose alginic acid in the mixture, and the mixture is stirred until the average molecular weight of alginic acid in the mixture is 40,000 daltons or less. stirred.

One hour after the alginate lyase was added to the mixture, 150 g of protease was added to the mixture to degrade proteins in the mixture. Here, as the proteolytic enzyme, a 1:1 mixture of alcalase and flavorzyme was used. Then, the mixture to which the protease was added was reacted with stirring for 2 hours.

Then, in order to stop the protein degradation, the mixture was heated at a temperature of 90° C. or higher for 2 hours and then allowed to stand.

Thereafter, the mixture was separated into solids and liquids using a filter press, and then concentrated and freeze-dried until the separated liquid had a sugar content of 15 Brix to obtain 26.5 kg of powder of a composite composition.

As can be seen from the above examples, cell destruction of seaweed greatly affects the recovery and extraction yield of physiologically active substances and nutrients. In addition, the amount of extraction of the complex composition varies according to the amount of purified water mixed into the pulverized seaweed and the amount of alginate and proteolytic enzymes. In other words, it can be seen that the ratio of seaweed and purified water, the ratio of seaweed and alginate, and the ratio of seaweed and protease are important variables affecting the extraction amount of the complex composition.

Although not shown in the drawings, the manufacturing apparatus for performing the extraction method of the present invention includes a freezing facility, a storage tank, a water supply means, a water discharge means, a stirring means, a solid-liquid separation means, and a sodium hydrogen carbonate supply means in addition to the already mentioned components. , a weight detection means, a sugar content detection means, a concentration means, a drying means, and the like, and includes a control unit for controlling the operation of these components and the overall manufacturing process.

The above description of the present invention is for illustration, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and likewise described as being distributed. The components that are present may also be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present invention.

Claims (10)

freezing the pulverized seaweed to destroy cells of the wet pulverized seaweed;
mixing the pulverized seaweed with a predetermined amount of water to create a mixture;
adding sodium bicarbonate to the mixture so that the pH of the mixture reaches a preset range;
adding a first degrading enzyme for decomposing the alginic acid to the mixture so that the average molecular weight of the alginic acid in the mixture is equal to or less than a reference value;
A method of extracting a complex composition from seaweed, comprising adding a second degrading enzyme for decomposing proteins in the mixture to the mixture. The method of claim 1,
In the step of adding sodium hydrogen carbonate to the mixture so that the pH of the mixture reaches a preset range,
A method for extracting a complex composition from seaweed, characterized in that sodium bicarbonate is added to the mixture so that the pH of the mixture reaches 5 to 7. The method of claim 1,
The method for extracting a complex composition from seaweed, characterized in that the first degrading enzyme is a gamma proteobacterial enzyme. The method of claim 1,
In the step of adding the first degrading enzyme to the mixture,
A method for extracting a complex composition from seaweed, characterized in that the first degrading enzyme corresponding to 0.05 to 0.1% by weight of seaweed is added to the mixture. The method of claim 1,
In the step of adding the first degrading enzyme to the mixture,
A method for extracting a complex composition from seaweed, characterized in that the first degrading enzyme is added to the mixture so that the average molecular weight of alginic acid in the mixture is 40,000 daltons or less. The method of claim 1,
The method of extracting a complex composition from seaweed, characterized in that the second degrading enzyme is an enzyme in which alcalase and flavorzyme are mixed in a 1:1 ratio. The method of claim 1,
In the step of adding the second degrading enzyme to the mixture,
A method for extracting a complex composition from seaweed, characterized in that the second degrading enzyme corresponding to 0.1 to 0.2% by weight of seaweed is added to the mixture. The method of claim 1,
In the step of adding the second degrading enzyme to the mixture,
A method for extracting a complex composition from seaweed, characterized in that after adding the first degrading enzyme to the mixture, the second degrading enzyme is added to the mixture after a predetermined time. The method of claim 1,
The method of extracting a complex composition from seaweed, characterized in that after adding the second degrading enzyme to the mixture, heating the mixture at a temperature of 90° C. or higher for 1 to 3 hours to stop protein degradation. The method of claim 1,
A method for extracting a complex composition from seaweed, characterized in that it further comprises the step of extracting the complex composition comprising fucoidan, alginic acid, amino acids, minerals, vitamins, fucosterol and beta-carotene from the mixture.

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