CN113831421B - Combined preparation method of grifola frondosa mycelium polypeptide and beta-glucan - Google Patents
Combined preparation method of grifola frondosa mycelium polypeptide and beta-glucan Download PDFInfo
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- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
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- C05G5/20—Liquid fertilisers
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- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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- C12P19/00—Preparation of compounds containing saccharide radicals
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- C12P19/08—Dextran
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- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
The invention belongs to the technical field of natural product extraction and separation, and particularly provides a joint preparation method of grifola frondosa mycelium polypeptide and beta-glucan. The fermented Grifola frondosa mycelia are used as raw materials, and processes such as ethanol degreasing, drying, superfine grinding, enzyme extraction, hot alkali extraction, neutralization, centrifugation, microfiltration, ultrafiltration desalination concentration, spray drying and the like are carried out to produce Grifola frondosa polypeptide and beta-glucan. The grifola frondosa extract prepared by the method has high content of polypeptide and beta-glucan, short production period and suitability for industrial production.
Description
Technical Field
The invention relates to the technical field of natural product extraction and separation, in particular to a combined preparation method of grifola frondosa mycelium polypeptide and beta-glucan.
Background
Grifola frondosa (Grifola frondosa) is a fungus belonging to the genera Grifola, polyporaceae, basidiomycetes, basidiomycotina, aphyllophorales, polyporaceae, grifola, also called Polyporus, sphaerotheca, marasma, lotus flower, etc., and is called Maitake (Maitake) in Japan. Its medicinal action is recorded in "Junzhou" of Japanese saka ran for curing hemorrhoid, and "Weigan, ping and non-toxic". The "harmonizing spleen and stomach and tranquilizing mind" efficacy is recorded in Shen nong Ben Cao Jing of Dong Han time. The Grifola frondosa mycelia have unique fragrance, and are rich in vitamins, minerals and bioactive substances. The grifola frondosa beta-glucan is grifola frondosa polysaccharide extracted and separated from grifola frondosa mycelia, consists of beta- (1-6) glucan with beta- (1-3) side chains and beta- (1-3) glucan with beta- (1-6) side chains, and can activate various immune cells and release cytokines, improve the immunity of an organism and relieve sub-health symptoms to a certain extent. The grifola frondosa polypeptide is a small molecular peptide obtained by hydrolyzing and separating protease, has the effects of reducing blood pressure, reducing blood fat, resisting fatigue, resisting bacteria, improving immunity, resisting oxidation and the like, and is easier to be absorbed by a human body compared with macromolecular protein.
The existing extraction technologies of the grifola frondosa extract comprise a water extraction method, an alkali extraction method, an enzyme extraction method, an ultrasonic wave auxiliary method, a microwave auxiliary method and the like, wherein the ultrasonic wave auxiliary method and the microwave auxiliary method have high requirements on equipment, high production energy consumption and high production cost. The water extraction method and enzyme extraction method can obtain Grifola frondosa extract with low content of beta-dextran. The alkali extraction method usually uses sodium hydroxide, the wastewater amount is large, and the obtained grifola frondosa extract has high beta-glucan content but low polypeptide content. Therefore, the technical problem to be solved in the art is how to provide a method for jointly extracting polypeptide and beta-glucan from grifola frondosa mycelia, which has low energy consumption and high polypeptide and beta-glucan yield.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a combined preparation method of grifola frondosa mycelium polypeptide and beta-glucan. The invention takes fermented grifola frondosa mycelia as a raw material, and processes such as ethanol degreasing, drying, superfine grinding, enzyme extraction, thermokalite extraction, neutralization, centrifugation, microfiltration, ultrafiltration desalination concentration, spray drying and the like are carried out to produce grifola frondosa polypeptide and beta-glucan. The grifola frondosa extract prepared by the method has high content of polypeptide and beta-glucan, short production period and suitability for industrial production.
The technical scheme of the invention is as follows: according to the method, firstly, ethanol degreasing is adopted to destroy cell walls of grifola frondosa mycelia, and then an ultra-micro crushing process is adopted to physically break the walls, so that when the enzymatic method and the alkali extraction method are combined for extraction in the next step, polypeptide and polysaccharide can be more easily extracted. Performing enzymolysis extraction by adopting protease to obtain an extracting solution with higher content of grifola frondosa polypeptide, then extracting with alkali to obtain an extracting solution with higher content of grifola frondosa beta-glucan, combining the extracting solutions, performing membrane treatment to remove macromolecular substances and micromolecular salts, and performing spray drying to obtain the grifola frondosa extract with high content of polypeptide and beta-glucan. The production alkali adopts potassium hydroxide to replace the traditional sodium hydroxide, and the produced wastewater can be used for developing liquid potash fertilizer.
The invention relates to a combined preparation method of grifola frondosa mycelium polypeptide and beta-glucan, which specifically comprises the following steps:
(1) Adding the fermented grifola frondosa mycelia and ethanol into an extraction container, stirring and degreasing for 12-24 h, centrifuging, drying, and carrying out superfine grinding;
the ethanol is preferably food grade 95% ethanol; after the ethanol is added, the mass percent of the ethanol is more than 75 percent.
Sieving the Grifola frondosa mycelium powder obtained by micronizing with 200 mesh sieve;
(2) Adding the grifola frondosa superfine powder and water into an extraction container, adding protease, stirring and extracting for 2-6 h, inactivating enzyme at 100 ℃ for 10min, cooling, centrifuging, respectively collecting clear liquid and solid, adjusting the pH of the clear liquid to 3.5-4.0 by using hydrochloric acid, centrifuging, and collecting the clear liquid for later use;
(3) Adding an alkali solution into the solid matter, extracting for 1-3 times at the temperature of 80-90 ℃ for 2h, combining the extracting solutions after the extraction is finished, neutralizing with acetic acid, centrifuging, collecting clear liquid, and precipitating for processing an organic fertilizer;
(4) Combining the centrifugal clear liquids in the steps (2) and (3), filtering by adopting a microfiltration membrane with the aperture of 0.2-0.5 mu m, desalting the permeate by using an ultrafiltration membrane with the aperture of 1kD, continuously adding purified water into the trapped liquid to elute and remove salt until the conductivity of the trapped liquid is less than 1mS/cm and the content of soluble solid matters is 2-3%, and stopping ultrafiltration;
(5) Spray drying the ultrafiltration retentate to obtain Grifola frondosa extract containing polypeptide and beta-glucan.
The water consumption in the step (2) is 10-40 times of the mass of the grifola frondosa superfine powder, the protease is alkaline protease or neutral protease or a mixture thereof, the enzyme activity is 20 ten thousand U/g, and the addition amount is 0.2-1% of the mass of the grifola frondosa superfine powder; before using the alkaline protease, potassium hydroxide is added to adjust the pH value to 8.5-10.5; before the mixture of alkaline protease and neutral protease is used, potassium hydroxide is added to adjust the pH value to 7.5-9.5.
In the step (3), the alkali solution is potassium hydroxide solution, and the concentration of the alkali solution is 5-10% (w/v); the volume of the added alkali solution is 10 to 30 times (ml/g) of the original mass of the grifola frondosa submicron powder.
The invention adopts the combined extraction process of wall breaking, enzyme method, alkaline method and membrane treatment for the first time in the production process of the grifola frondosa extract, mycelium obtained after fermentation of grifola frondosa directly adopts ethanol degreasing to destroy cell walls, the degreased grifola frondosa mycelium adopts an ultramicro crushing process to carry out physical wall breaking after drying, simultaneously, the ethanol degreasing and the ultramicro crushing also lead protein to be denatured, protein molecules are changed into disordered loose extending structures from ordered compact structures, the protein molecules are easier to be hydrolyzed by protease, and polypeptide and polysaccharide are easier to be extracted.
The enzymatic extraction process mainly comprises the steps of obtaining an extracting solution with high content of grifola frondosa polypeptide, and removing macromolecular protein in grifola frondosa mycelia after enzymatic extraction, so that beta-glucan is easier to extract in the alkali extraction process. The alkali extraction process mainly obtains the extract with higher beta-glucan content of the grifola frondosa. Mixing extractive solutions, treating with membrane, removing macromolecular substances and micromolecular salt, and spray drying to obtain Grifola frondosa extract containing high polypeptide and beta-dextran. The process has better synergistic effect for extracting the beta-glucan, and simultaneously reduces the dosage of alkali, and the combination is the first application in the field and has obvious improvement compared with the prior art.
In addition, the invention adopts potassium hydroxide to replace sodium hydroxide in the alkali extraction process, and can realize the application of the waste water in the liquid fertilizer.
In conclusion, the combined preparation method of the grifola frondosa mycelium polypeptide and the beta-glucan adopts combined processes of wall breaking, enzyme extraction, alkali extraction, membrane treatment and the like, so that the alkali consumption is greatly reduced, and the yield of the polypeptide and the beta-glucan is improved. Potassium hydroxide is adopted to replace sodium hydroxide, the wastewater contains potassium acetate, and sylvite can be applied to the preparation of liquid fertilizer, so that the problem that the wastewater is difficult to treat is solved. The yield of the grifola frondosa extract obtained by the method can reach more than 11%, wherein the polypeptide content is higher than 30%, the beta-glucan content is higher than 60%, and the yield is far higher than that of the prior art.
Detailed Description
The present invention will be described in further detail with reference to the following examples, but it should not be construed that the scope of the present invention is limited to the examples. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention.
Example 1
(1) Adding the fermented grifola frondosa mycelia and food-grade 95% ethanol into an extraction container, stirring and degreasing for 12-24 h, centrifuging and drying, wherein the final mass percent of the ethanol is more than 75%. Pulverizing the dried Grifola frondosa mycelia by an ultrafine pulverizer to obtain Grifola frondosa mycelia powder, and sieving with 200 mesh sieve.
(2) Accurately weighing 100g of grifola frondosa submicron powder, adding 3kg of purified water, adding potassium hydroxide to adjust the pH value to 8.5-10.5, adding 0.5g of alkaline protease with enzyme activity of 20 ten thousand U/g, stirring and extracting for 5h, inactivating enzyme at 100 ℃ for 10min, cooling, centrifuging, respectively collecting clear liquid and solid matter, adjusting the pH value of the clear liquid to 3.5-4.0 by hydrochloric acid, centrifuging, and collecting the clear liquid for later use.
(3) Adding 2L of 9% potassium hydroxide solution into the solid, extracting at 85 deg.C for 2 hr for 2 times, mixing extractive solutions, neutralizing with acetic acid, centrifuging, and collecting clear solution.
(4) And (4) combining the enzyme extraction in the step (2) and the centrifugal clear liquid of the alkali extraction in the step (3), filtering by adopting a microfiltration membrane with the aperture of 0.5 mu m, desalting the permeate by using a 1kD ultrafiltration membrane, continuously adding pure water into the trapped fluid to elute and remove salt until the conductivity of the trapped fluid is less than 1mS/cm and the content of soluble solids is 2-3%, and stopping ultrafiltration.
(5) Spray drying the ultrafiltration retentate to obtain Grifola frondosa extract containing polypeptide and beta-glucan.
The yield of the Grifola frondosa extract obtained by the method is 12.02% based on the dry weight of Grifola frondosa mycelia, wherein the content of polypeptide is 33.28%, and the content of beta-glucan is 60.5%.
Example 2
(1) Adding the fermented grifola frondosa mycelium and food-grade 95% ethanol into an extraction container, wherein the final mass percent of the ethanol is more than 75%, stirring and degreasing for 12-24 h, centrifuging and drying. Pulverizing the dried Grifola frondosa mycelia by an ultrafine pulverizer to obtain Grifola frondosa mycelia powder, and sieving with 200 mesh sieve.
(2) Accurately weighing 100g of grifola frondosa submicron powder, adding 2.5kg of purified water, adding 1g of neutral protease with enzyme activity of 20 ten thousand U/g, stirring and extracting for 6h, inactivating the enzyme at 100 ℃ for 10min, cooling, centrifuging, respectively collecting a clear liquid and a solid matter, adjusting the pH of the clear liquid to 3.5-4.0 by hydrochloric acid, centrifuging, and collecting the clear liquid for later use.
(3) Adding 3L of 7% potassium hydroxide solution into the solid, extracting at 85 deg.C for 2 hr for 2 times, mixing extractive solutions, neutralizing with acetic acid, centrifuging, and collecting clear solution.
(4) Combining the centrifugal clear liquid of the enzyme extraction and the alkali extraction, filtering by adopting a microfiltration membrane with the aperture of 0.2 mu m, desalting the permeate by using a 1kD ultrafiltration membrane, continuously adding purified water into the trapped liquid to elute and remove salt until the conductivity of the trapped liquid is less than 1mS/cm and the content of soluble solid matters is 2-3%, and stopping ultrafiltration.
(5) Spray drying the ultrafiltration retentate to obtain Grifola frondosa extract containing polypeptide and beta-glucan.
The yield of Grifola frondosa extract was 11.16% based on dry weight of Grifola frondosa mycelia, wherein the content of polypeptide was 29.05%, and the content of beta-glucan was 64.68%.
Example 3
(1) Adding the fermented grifola frondosa mycelium and food-grade 95% ethanol into an extraction container, wherein the final mass percent of the ethanol is more than 75%, stirring and degreasing for 12-24 h, centrifuging and drying. Pulverizing the dried Grifola frondosa mycelia by an ultrafine pulverizer to obtain Grifola frondosa mycelia powder, and sieving with 200 mesh sieve.
(2) Accurately weighing 100g of grifola frondosa ultrafine powder, adding 2kg of purified water, adding potassium hydroxide to adjust the pH value to 7.5-9.5, adding 1.0g of mixed enzyme of alkaline protease and neutral protease with the enzyme activity of 20 ten thousand U/g, mixing the alkaline protease and the neutral protease according to a mass ratio of 1.
(3) Adding 2.5L potassium hydroxide solution with mass concentration of 8% into the solid, extracting at 90 deg.C for 2 hr for 2 times, combining the extractive solutions, neutralizing with acetic acid, centrifuging, and collecting the clear solution.
(4) Combining the centrifugal clear liquid of the enzyme extraction and the alkali extraction, adopting a microfiltration membrane with the aperture of 0.5 mu m for filtration, desalting the permeate by using a 1kD ultrafiltration membrane, continuously adding pure water into the trapped liquid for eluting and desalting until the conductivity of the trapped liquid is less than 1mS/cm, the content of soluble solids is 2-3%, and stopping ultrafiltration.
(5) Spray drying the ultrafiltration retentate to obtain Grifola frondosa extract containing polypeptide and beta-glucan.
The yield of Grifola frondosa extract based on dry weight of Grifola frondosa mycelia is 12.25%, wherein the content of polypeptide is 30.47%, and the content of beta-glucan is 62.79%.
Example 4
(1) Adding the fermented grifola frondosa mycelia and food-grade 95% ethanol into an extraction container, stirring and degreasing for 12-24 h, centrifuging and drying, wherein the final mass percent of the ethanol is more than 75%. Pulverizing the dried Grifola frondosa mycelia by an ultrafine pulverizer to obtain Grifola frondosa mycelia powder, and sieving with 200 mesh sieve.
(2) Accurately weighing 100g of grifola frondosa superfine powder, adding 3kg of purified water, adding potassium hydroxide to adjust the pH value to 8.5-10.5, adding 0.2g of alkaline protease with the enzyme activity of 20 ten thousand U/g, stirring and extracting for 3h, inactivating the enzyme for 10min at 100 ℃, cooling, centrifuging, respectively collecting clear liquid and solid matters, adjusting the pH value of the clear liquid to 3.5-4.0 by hydrochloric acid, centrifuging, and collecting the clear liquid for later use.
(3) Adding 3.5L of 6% potassium hydroxide solution into the solid, extracting at 87 deg.C for 2 hr for 1 time, mixing extractive solutions, neutralizing with acetic acid, centrifuging, and collecting clear liquid.
(4) Combining the centrifugal clear liquid of the enzyme extraction and the alkali extraction, adopting a microfiltration membrane with the aperture of 0.2 mu m for filtration, desalting the permeate by using a 1kD ultrafiltration membrane, continuously adding pure water into the trapped liquid for eluting and desalting until the conductivity of the trapped liquid is less than 1mS/cm, the content of soluble solids is 2-3%, and stopping ultrafiltration.
(5) Spray drying the ultrafiltration retentate to obtain Grifola frondosa extract containing polypeptide and beta-glucan.
The yield of the Grifola frondosa extract based on the dry weight of the Grifola frondosa mycelium is 11.85%, wherein the polypeptide content is 35.42%, and the beta-glucan content is 58.06%.
Example 5
(1) Adding the fermented grifola frondosa mycelia and food-grade 95% ethanol into an extraction container, stirring and degreasing for 12-24 h, centrifuging and drying, wherein the final mass percent of the ethanol is more than 75%. Pulverizing dried Grifola frondosa mycelia with a micronizer to obtain Grifola frondosa mycelia powder, and sieving with 200 mesh sieve.
(2) Accurately weighing 100g of grifola frondosa superfine powder, adding 4kg of purified water, adding 1g of neutral protease with 20 ten thousand U/g of enzyme activity, stirring and extracting for 5.5h, inactivating enzyme at 100 ℃ for 10min, cooling, centrifuging, respectively collecting clear liquid and solid, regulating the pH of the clear liquid to 3.5-4.0 with hydrochloric acid, centrifuging, and collecting the clear liquid for later use.
(3) Adding 1L of 10% potassium hydroxide solution into the solid, extracting at 90 deg.C for 2 hr for 3 times, mixing extractive solutions, neutralizing with acetic acid, centrifuging, and collecting clear solution.
(4) Combining the centrifugal clear liquid of the enzyme extraction and the alkali extraction, adopting a microfiltration membrane with the aperture of 0.5 mu m for filtration, desalting the permeate by using a 1kD ultrafiltration membrane, continuously adding pure water into the trapped liquid for eluting and desalting until the conductivity of the trapped liquid is less than 1mS/cm, the content of soluble solids is 2-3%, and stopping ultrafiltration.
(5) Spray drying the ultrafiltration retentate to obtain Grifola frondosa extract containing polypeptide and beta-glucan.
The yield of Grifola frondosa extract based on dry weight of Grifola frondosa mycelium was 12.18%, wherein the content of polypeptide was 28.86%, and the content of beta-glucan was 65.11%.
Comparative example 1
Accurately weighing 100g of Grifola frondosa superfine powder, adding 2L of 10% potassium hydroxide solution, extracting at 85 deg.C for 2 hr for 2 times, mixing extractive solutions, neutralizing with acetic acid, centrifuging, and collecting clear liquid. Filtering with a microfiltration membrane with the aperture of 0.5 mu m, desalting the permeate with a 1kD ultrafiltration membrane, continuously adding purified water into the retentate to elute and remove the salt until the conductivity of the retentate is less than 1mS/cm and the content of soluble solids is 2-3%, and spray-drying the ultrafiltration retentate to obtain the grifola frondosa extract containing the polypeptide and the beta-glucan. The yield of the grifola frondosa extract is 4.68%, wherein the polypeptide content is 8.72%, and the beta-glucan content is 83.41%.
Comparative example 2
Accurately weighing 100g of grifola frondosa superfine powder, adding 4L of purified water, adding potassium hydroxide to adjust the pH value to 8.5-10.5, adding 0.3g of alkaline protease with enzyme activity of 20 ten thousand U/g, stirring and extracting for 5h, inactivating enzyme for 10min at 100 ℃, cooling, centrifuging, respectively collecting clear liquid and solid matters, adjusting the pH value of the clear liquid to 3.5-4.0 by hydrochloric acid, centrifuging, collecting the clear liquid, filtering the clear liquid by adopting a microfiltration membrane with the pore diameter of 0.2 mu m, desalting permeate by adopting an ultrafiltration membrane of 1kD, continuously adding purified water into trapped liquid to elute and remove salt until the conductivity of the trapped liquid is less than 1mS/cm and the content of soluble solid matters is 2-3%, and spray-drying the trapped liquid of ultrafiltration to obtain the grifola extract containing polypeptide and beta-glucan. The yield of the grifola frondosa extract is 4.06%, wherein the polypeptide content is 76.85%, and the beta-glucan content is 10.26%.
Therefore, the combined preparation method of the grifola frondosa mycelium polypeptide and the beta-glucan, which is adopted by the invention, has great improvement on the yield of the grifola frondosa extract and better popularization and application values.
Claims (3)
1. A combined preparation method of grifola frondosa mycelium polypeptide and beta-glucan is characterized by comprising the following steps:
(1) Adding the fermented grifola frondosa mycelia and ethanol into an extraction container, stirring and degreasing for 12-24 h, centrifuging, drying, and carrying out superfine grinding;
(2) Adding the grifola frondosa superfine powder and water into an extraction container, adding protease, stirring and extracting for 2-6 h, inactivating enzyme at 100 ℃ for 10min, cooling, centrifuging, respectively collecting clear liquid and solid, adjusting the pH of the clear liquid to 3.5-4.0 by using hydrochloric acid, centrifuging, and collecting the clear liquid for later use;
(3) Adding an alkali solution into the solid, extracting at the temperature of 80-90 ℃ for 1-3 times, combining the extracting solutions after the extraction is finished, neutralizing with acetic acid, centrifuging, and collecting clear liquid;
(4) Combining the centrifugal clear liquids in the steps (2) and (3), filtering by adopting a microfiltration membrane with the aperture of 0.2-0.5 mu m, desalting the permeate by using an ultrafiltration membrane with the aperture of 1kD, continuously adding purified water into the trapped liquid to elute and remove salt until the conductivity of the trapped liquid is less than 1mS/cm and the content of soluble solid matters is 2-3%, and stopping ultrafiltration;
(5) Spray drying the ultrafiltration retentate to obtain Grifola frondosa extract containing polypeptide and beta-glucan;
the protease in the step (2) is alkaline protease or neutral protease or a mixture thereof;
the protease in the step (2) has the enzyme activity of 20 ten thousand U/g, the addition amount is 0.2-1% of the mass of the grifola frondosa superfine powder, and before the alkaline protease is used, potassium hydroxide is added to adjust the pH value to 8.5-10.5;
in the step (3), the alkali solution is potassium hydroxide solution, and the concentration of the alkali solution is 5-10%; the volume of the added alkali solution is 10 to 30 times of the mass of the original grifola frondosa superfine powder;
before the mixture of alkaline protease and neutral protease is used, potassium hydroxide is added to adjust the pH value to 7.5-9.5.
2. The combined preparation method of grifola frondosa mycelium polypeptide and beta-glucan according to claim 1, wherein the amount of water used in step (2) is 10-40 times of the weight of the grifola frondosa superfine powder.
3. The method for preparing grifola frondosa mycelium polypeptide and beta-glucan in a combined manner as claimed in claim 1, wherein the ethanol added in step (1) is more than 75% by weight.
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| US6835558B2 (en) * | 2002-02-04 | 2004-12-28 | General Mills, Inc. | Beta-glucan compositions and process therefore |
| CN1398898A (en) * | 2002-08-28 | 2003-02-26 | 维京仲华（上海）生物医药科技有限公司 | Extraction and separation process of ash tree flower sporophore polyglycopeptide |
| CN1663959A (en) * | 2004-03-27 | 2005-09-07 | 中国海洋大学 | Beta-glucan peptide and its preparing process and application |
| CN1255429C (en) * | 2004-10-20 | 2006-05-10 | 上海市农业科学院 | Method for preparing Griflola frondosa proteoglycan |
| CN104757252B (en) * | 2015-04-22 | 2018-03-09 | 福建农林大学 | A kind of preparation method of the grifola frondosus protein zymolyte with antioxidation activity |
| CN105001352B (en) * | 2015-07-14 | 2017-04-26 | 青岛海大海洋生物医药销售有限公司 | Beta-1,3/1,6-glucan, preparation method therefor, and application thereof in preparing immune enhancement and anti-tumor medicine and functional food |
| CN107541533B (en) * | 2017-06-13 | 2021-03-19 | 湖南民康生物技术研究所 | Preparation method of medicinal and edible fungi hypha polysaccharide polypeptide immunopotentiator |
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| CN101805774A (en) * | 2010-03-12 | 2010-08-18 | 南通康麦生物科技有限公司 | Method for comprehensively extracting oat polypeptide and oat glucan |
| CN104894199A (en) * | 2015-05-04 | 2015-09-09 | 中国农业科学院农产品加工研究所 | Yeast cell short peptide and yeast cell wall polysaccharide synchronous preparation method |
| CA3003536A1 (en) * | 2015-12-07 | 2017-06-15 | Novozymes A/S | Polypeptides having beta-glucanase activity, polynucleotides encoding same and uses thereof in cleaning and detergent compositions |
| CN109680027A (en) * | 2018-12-28 | 2019-04-26 | 中国药科大学 | A kind of grifola frondosus small peptide iron chelate and its preparation method and application |
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