KR20220090373A - Health supplement food composition for improving respiratory diseases and health supplement food using the same - Google Patents

Abstract

The present invention relates to a health supplement composition for improving bronchial function and a health supplement using the same.
The dietary supplement composition for improving bronchial function according to the present invention includes red pepper cod extract, mugwort extract, algae extract, green tea extract, egg yolk oil and antler collagen.
According to the above configuration, the present invention is effective in improving bronchial functions such as colds, coughs, phlegm, and fever by composing a health supplement composition containing natural herbal ingredients as active ingredients, and can be safely taken without side effects on the human body. It is possible to manufacture health supplements that can provide nutrients to lead a healthy life.

Description

Health supplement composition for improving bronchial function and health supplement using the same

The present invention relates to a health supplement composition for improving bronchial function and a health supplement using the same, and more particularly, to a health supplement composition comprising natural herbal ingredients as an active ingredient to form a health supplement composition for colds, coughs, phlegm, fever, etc. It relates to a health supplement composition using herbal materials that is effective in improving the bronchial function of the body and can be safely taken without side effects to the human body, and can provide nutrients to lead a healthy life, and a health supplement using the same.

Modern people are exposed to allergic bronchial diseases caused by air pollution, allergens (animal hair, pollen, etc.) and smoking due to the development of industry. It is known as one of the most frequent diseases in

A cold is a disease that causes a runny nose and stuffy nose in all age groups. Bronchitis is an infectious disease caused by infection with Mycobacterium tuberculosis. Chronic bronchitis is an inflammatory disease that occurs in the lung tissue. Chronic bronchitis refers to a disease in which the bronchial mucosa is narrowed by a fibrotic inflammatory reaction due to inflammation of the capillary bronchus, and the airway is blocked by the secretion of mucus from the mucosal cells.

The sputum is usually white or slightly yellow in color and tends to come out in the morning. As the disease progresses, the difficulty in breathing gradually worsens over a period of several months to years, making it difficult to breathe even with a little activity. By cause, there are allergic, infectious, traumatic, etc., and pathologically there are catarrhal, purulent, ulcerative, infiltrating, and the like. The most common cause is bacterial, viral, or fungal infection, and even people who are not usually infected are prone to infection when the body's resistance is weakened.

The above diseases, such as colds, bronchitis, tuberculosis, pneumonia, chronic bronchitis, and phlegm, have some differences in their causes and symptoms, and are treated by taking medications depending on the disease, but the effect of improving bronchial function is only temporary. , there were difficulties in long-term treatment due to the insignificant effect of symptom improvement, as well as the side effects and tolerance of synthetic chemical drugs.

Accordingly, by using medicinal plants of natural ingredients without problems such as side effects, it is effective in improving bronchial functions such as colds, coughs, sputum, and fever, as well as health assistance for improving bronchial function that can be safely taken without side effects in the human body Food development is urgently needed.

Domestic Registered Patent No. 10-1731899 (registered on April 25, 2017) Domestic Registered Patent No. 10-2034238 (registered on October 14, 2019) Domestic Patent Publication No. 10-2020-0048459 (published on May 08, 2020)

The present invention is effective in improving bronchial functions such as colds, coughs, phlegm, and fever by composing a health supplement composition containing natural herbal ingredients as active ingredients, and can be safely taken without side effects to the human body and can lead a healthy life. An object of the present invention is to provide a health supplement composition using a herbal material that can provide nutrients so as to provide nutrients and a health supplement food using the same.

Various problems to be solved by the present invention are not limited to the above-mentioned problems, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

The dietary supplement composition for improving bronchial function according to the present invention includes an extract of Gochujang root, an extract of Injin mugwort, an extract of algae, a green tea extract, egg yolk oil, and antler collagen.

In a weight ratio of 3 to 7 parts by weight of the red pepper cod extract, 2 to 4 parts by weight of the Injin mugwort extract, 1 to 5 parts by weight of the algae extract, 2 to 4 parts by weight of the seaweed extract, 1 to 3 parts by weight of egg yolk oil, and 0.5 to 1.5 parts by weight of the antler collagen. may be included.

In addition to the composition consisting of the red pepper cod extract, ginseng mugwort extract, shellfish extract, green tea extract, egg yolk oil and antler collagen, it may further include hoesaengi capsicum extract, green-lipped mussel oil, and Sanghwang mushroom extract.

3 to 7 parts by weight of the red pepper root extract, 2 to 4 parts by weight of the Injin mugwort extract, 1 to 5 parts by weight of the algae extract, 2 to 4 parts by weight of the green tea extract, 1 to 3 parts by weight of egg yolk oil, and 0.5 to 1.5 parts by weight of the antler collagen, the above It may be further included in a weight ratio of 0.5 to 0.9 parts by weight of hoesaengi mother and half extract, 0.3 to 0.7 parts by weight of green lipped mussel oil, and 0.1 to 0.5 parts by weight of Sanghwang mushroom extract.

In addition, the present invention discloses a health supplement for improving bronchial function prepared by including the composition.

Specific details of other embodiments are included in the detailed description.

The present invention is effective in improving bronchial functions such as colds, coughs, phlegm, and fever by composing a health supplement composition containing natural herbal ingredients as active ingredients, and can be safely taken without side effects to the human body and can lead a healthy life. It is possible to manufacture health supplements that can provide nutrients to

It will be fully understood that embodiments of the technical idea of the present invention may provide various effects not specifically mentioned.

Advantages and features of the present invention, and methods for achieving them, will become apparent with reference to the embodiments described below in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided so that the disclosed subject matter may be thorough and complete, and that the spirit of the present invention may be sufficiently conveyed to those skilled in the art.

The terms used in the present application are only used to describe specific embodiments, and are not intended to limit the present invention. The singular expression includes the plural expression unless the context clearly dictates otherwise.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related art, and should not be interpreted in an ideal or excessively formal meaning unless explicitly defined in the present application. does not

Hereinafter, a preferred embodiment of the dietary supplement composition for improving bronchial function according to the present invention will be described in detail.

The dietary supplement composition for improving bronchial function according to the present invention includes an extract of Gochujang root, an extract of Injin mugwort, an extract of algae, a green tea extract, egg yolk oil, and antler collagen.

In addition, the health supplement composition for improving bronchial function according to the present invention is 3 to 7 parts by weight of red pepper root extract, 2 to 4 parts by weight of Injin mugwort extract, 1 to 5 parts by weight of algae extract, 2 to 4 parts by weight of green tea extract, and 1 part by weight of egg yolk oil. It may be included in a weight ratio of 3 to 3 parts by weight and 0.5 to 1.5 parts by weight of antler collagen.

The red pepper cod extract may be prepared so that the useful components of red pepper cod are effectively extracted by extracting the red pepper cod.

The red pepper paste contains various nutrients such as vitamins, beta-carotene, and calcium, as well as various minerals. It has excellent inhibitory effect and can be used safely without side effects on the human body.

In general, when infected with a virus, it is accompanied by fever, cough, sore throat, pneumonia, difficulty in breathing, etc., and the red pepper root extract is effective for the above-mentioned viral diseases.

The Injin mugwort extract can be prepared by extracting the Injin mugwort extract to effectively contain the useful ingredients of the Injin mugwort. It is known to contain (flavonoids), caffeic acid, aromatic oxycarbonic acid, and various minerals and vitamins. , expectorant, indigestion, menstrual disorders, pregnancy poisoning, etc. have been used for the treatment and prevention of various diseases.

The major pharmacological components of Injin mugwort are scoparone (6,7-dimethoxycumarine) and betaine. It is used as an agent and diuretic, and is known to have high antioxidant activity.

The Liliaceae extract may be prepared so as to effectively contain the useful components of the Liliaceae by extracting from the Liliaceae, the Fritillaria thunbergii belongs to the Liliaceae, and the Latin name is Fritillariae Bulbus. The shell includes a planer from which the core is removed and the main plate from which the core is not removed. The planer is a scaly stem, and the single scaly leaf of the outer layer of the scaly stem is almost crescent-shaped, and has a height of 1 to 2 cm, a width of 20 to 35 mm, the outer surface is milky white to light yellow, and the inner surface is white or light brown and contains white powder. Covered.

The bulbs of Fritillaria thunbergii have anticancer effects and hypotensive effects such as tumors and breast cancer, and are effective in cough, sputum, pneumonia, tonsillitis, sore throat, bronchitis, dry mouth, cuts, boils, It is known to be effective against insect bites.

For example, the red pepper cod extract, the Injin mugwort extract, and the moss extract can be prepared by extracting the red pepper cod, Injin mugwort, and moss extract using a variety of extraction methods such as hot water extraction, organic solvent extraction, ultrasonic extraction, supercritical extraction, and the like. , The configuration of extracting red peppercorns extract, wormwood extract, and extracting paprika is a known technique, and detailed description thereof will be omitted for convenience of explanation and clarity of the technical idea of the present invention.

The green tea extract can be extracted from green tea to effectively contain useful components of green tea, and the green tea (Salicornia Hernacea L) is an annual herb belonging to the family Chenopodiaceae and is a plant that grows a lot in mudflats in the southwest coast of Korea. , in particular, it is a plant that grows in groups with Namunjae or Chickweed in the vicinity where seawater comes and closes, in the place made by reclamation, or in the vicinity of salt farms.

The green tea is called by various names depending on its ingredients, shape, and growth characteristics. In the old Chinese medical book <Shinnongchobonjing>, it was called green tea (鹹草) or salted vinegar (鹽草) because it had a very salty taste.

Green tea, a tidal flat plant, is known to not only accumulate a large amount of salt in the body, but also contain a large amount of various minerals. It contains a large amount of about 50%, and essential amino acids are reported to contain about 40% of the total amino acid content.

In addition, the green tea is known to be effective in cancer, sinusitis, arthritis, hypertension, back pain, obesity, hemorrhoids, diabetes, thyroiditis, asthma, bronchitis, and liver disease as a resource plant that has been widely used as a folk remedy since ancient times.

The green tea extract prepared by the following method may be used.

In order to prepare the green tea extract, first, it can be washed after preparing the green tea.

Next, the washed green tea can be immersed in the mixed solution.

In the above step, by immersing the washed green tea in a mixed solution, it is possible to prevent discoloration or easy deterioration of green tea, and the mixed solution may consist of grape juice, lemon juice and bamboo salt water.

In addition, in the above step, the grape juice is good for preventing blood clots and preventing adult diseases such as high blood pressure, arteriosclerosis and heart disease, and helps to improve memory. It is effective in preventing colds, headaches, etc., and the bamboo salt water sterilizes the green tea to prevent easy spoilage.

In addition, the lemon juice and bamboo salt water can prevent the green tea from discoloring over time, and the mixed solution is prepared by mixing 1 part by weight of grape juice, 1 part by weight of lemon juice, and 8 parts by weight of bamboo salt solution, The bamboo salt water may have a concentration of 1 to 3% by weight.

In addition, in the above step, the washed green tea can be immersed in a mixed solution and maintained at a temperature of 20 to 25° C. for 50 to 100 minutes.

Next, after taking out the green tea immersed in the mixed solution, the green tea can be steamed with steam.

In the above step, the green tea impregnated with the components of grape juice, lemon juice and bamboo salt water is mixed with a mixture of cinnamon powder and sugar, and then steamed in a steamer. In the above step, the cinnamon powder and sugar are cinnamon powder 20 to 30 parts by weight and 10 to 20 parts by weight of sugar are mixed, and heated in a steamer with steam at 120 to 140° C. for 20 to 40 minutes and steamed.

In the above step, the cinnamon powder enhances immunity and promotes blood circulation to help prevent vascular diseases, and has food poisoning prevention, sterilization and insecticidal effects, and the sugar enhances the surface gloss and sugar content of immersed green tea, and has an antiseptic effect. Storage can be improved.

Then, by extracting the steamed green tea, it is possible to prepare a green tea extract.

The extraction of the steamed green tea in the above step can be extracted using various extraction methods such as hot water extraction, organic solvent extraction, ultrasonic extraction, and supercritical extraction. For the sake of convenience of description and clarity of the technical idea of the present invention, a detailed description thereof will be omitted.

The yolk oil may be obtained from egg yolk, and may be obtained from egg yolks such as duck eggs and ostrich eggs as well as chicken eggs.

The egg yolk oil contains about 30% of lecithin, a component of the cell membrane, and other vitamins A and E, linoleic acid, and the like. Lecithin, also called phospholipid, has hydrophilic and lipophilic properties, so it has an emulsifying power to make milk-like by mixing water and oil well. It is known to have an anti-obesity effect by promoting

In addition, the egg yolk oil is rich in vitamins, minerals, taurine and unsaturated fatty acids, thereby forming an appropriate skin protective film for damaged skin and imparting moisturizing power and antioxidant functionality, thereby improving the skin.

In the present invention, the egg yolk oil prepared by the following method may be used.

In order to prepare the egg yolk oil, first, an egg or duck egg may be prepared and then boiled in water.

In the above step, the use of eggs or duck eggs is described as an example, but the technical idea of the present invention is not limited to the above eggs or duck eggs, and eggs of various known birds such as goose eggs and ostrich eggs may be used.

Next, after removing the shell and egg white of the boiled egg or duck egg, it can be prepared by separating the yolk.

Next, the prepared egg yolk may be first dried.

In the primary drying in the above step, the moisture remaining in the prepared egg yolk may be primarily removed by drying the prepared egg yolk at a temperature of 60 to 70° C. for 5 to 7 hours.

Then, after the primary dried egg yolk is pulverized to prepare an egg yolk powder, the egg yolk powder may be secondarily dried.

In the above step, after the primary dried egg yolk is pulverized and powdered to prepare an egg yolk powder, the secondary drying may be performed by applying hot air at a temperature of 120 to 140° C. to the egg yolk powder for 1 to 3 hours.

Next, the secondary dried egg yolk powder can be enzymatically decomposed by mixing and storing the enzyme and acid.

That is, in the above step, the enzyme and acid are mixed with the secondary dried egg yolk powder, and then the egg yolk powder mixed with the enzyme and acid is stored at a temperature of 38 to 40° C. for 10 to 20 hours to enzymatically decompose.

In the above step, known commercially available protease and xylanase are used as the enzyme as the enzyme, and citric acid and ascorbic acid may be used as the acid. Specifically, the secondary A weight ratio of 0.05 to 0.1 parts by weight of the protease, 0.005 to 0.01 parts by weight of Xylanase, 0.01 to 0.1 parts by weight of citric acid, and 0.01 to 0.1 parts by weight of ascorbic acid based on 100 parts by weight of the whole dried egg yolk powder can be mixed with

Next, a lactic acid bacteria culture solution is mixed with the enzymatically decomposed egg yolk powder and then fermented to prepare a fermented egg yolk powder.

In the above step, after mixing in a weight ratio of 1 to 3 parts by weight of the lactic acid bacteria culture solution to 100 parts by weight of the enzymatically decomposed egg yolk powder, fermentation may be performed at a temperature of 40 to 42° C. for 20 to 40 hours.

In addition, in the above step, the lactic acid bacteria culture medium was adjusted to pH 2.8 to 3.2 using 1.2N HCl, and then the lactic acid strain was separated from kimchi, and the separated lactic acid strain was used in MRS broth (Oxoid, England). After culturing at 37-39°C for 20-25 hours, dilute to 1×1.0 ^8-5 ×1.0 ⁸ CFU/mL, and then centrifuge at 10,000-15,000 rpm for 10-15 minutes to separate only the supernatant And, after filtering the supernatant (Supernatant) with a 0.45㎛ syringe filter (syringe filter), mixed in a weight ratio of 1,000 to 2,000 parts by weight of sterilized distilled water with respect to 100 parts by weight of the filtered supernatant It is prepared by diluting, the lactic acid bacteria As a lactic acid strain isolated from fermented food, kimchi, specifically, the lactic acid strain includes Lactobacillus curvatus , Weissella viridescens , Lactobacillus plantarum ) and Any one or more known lactic acid strains selected from the group consisting of Leuconostoc lactis may be used.

Then, the fermented egg yolk powder may be extracted by a supercritical extraction method to prepare egg yolk oil.

In the above step, the supercritical extraction method is to extract using a supercritical fluid, and the supercritical fluid is a term set to distinguish a state of a substance having a temperature and pressure above a critical point from a gas or a liquid. In a supercritical fluid, various physical properties change significantly near the critical point, and in particular, the change in density is closely related to the ability to dissolve various substances. The density of a supercritical fluid is similar to that of a liquid, but the viscosity is as low as that of a gas, and the diffusion coefficient is hundreds or thousands of times greater than that of a liquid. In other words, supercritical fluids have the advantages of both liquid and gas.

When a supercritical fluid is used as a solvent, it has the ability to dissolve a liquid or solid like a general liquid solvent, and by changing the pressure and temperature, the dissolving power can be adjusted, and the extraction solvent and the solute can be easily separated after extraction. In addition, since the supercritical fluid has a small viscosity like gas, the sample penetration is good, so the extraction efficiency is improved, and since the diffusion coefficient is large, the equilibrium can be quickly approached. In addition, by extracting at a relatively low temperature, heat damage can be avoided, a pollution-free process that does not pollute the environment is possible, and supercritical carbon dioxide is harmless, odorless, and non-flammable.

In the present invention, the supercritical extraction method is preferably performed using supercritical carbon dioxide as a solvent under conditions such as a temperature of 60 to 70° C., 400 to 500 atmospheres, and a S/F ratio (kg/Feed kg) of supercritical fluid of 35. More preferably, one or more cosolvents selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether may be extracted using a solvent in which supercritical carbon dioxide is added as a solvent, wherein the cosolvent is It is preferable to contain as much as 20 to 30% by volume based on the total volume of the solvent. After supercritical extraction, if supercritical carbon dioxide added with a small amount of cosolvent is flowed through the sample remaining in the extraction vessel, components that were not extracted with pure supercritical carbon dioxide alone may be extracted.

The antler collagen may be prepared from antlers, which are keratinized antlers of various deer such as hemp or plum belonging to the deer family. Ankak is salty in taste, warm in nature, and non-toxic. It enters the liver and kidneys. Ankak promotes blood circulation, eliminates blood stasis, helps kidney and liver functions, and contains a large amount of calcium to make bones strong.

In these antlers, sialic acid, hexose, pentose, hexosamine and uronic acid, free amino acids and minerals, and special fatty acids widely distributed in animal tissues, It has been reported that prostaglandins and gangliosides, a type of glycolipids, exist.

The sialic acid is a generic term for acyl derivatives of neuraminic acid, and more than 20 species are currently known in the animal world. It has been reported to be an acidic sugar that is involved in important physiological functions such as metastasis. It exists only temporarily in the free form in the metabolic process, but most of it exists in the form of an oligosaccharide by being linked to the non-reducing end of the sugar chain as a glycosidic.

The ganglioside is one of glycosphingolipids, and is an amphoteric substance composed of a hydrophilic sugar moiety and a hydrophobic ceramide, and at least one N-acetylneuraminic acid (sialic acid). ), which exists in the central nervous system and is involved in various functions of nerve functions and cell membranes. In addition, it is known to be effective in reducing cancer cells to normal cells, improving immunity, enhancing concentration, improving memory, and improving arthritis.

In the present invention, the anthorn collagen produced by the following method may be used.

In order to prepare the antlers collagen, first, the antlers may be prepared and then washed and cut into pieces of a predetermined length.

In the above step, the antler refers to ossified antlers of a deer. For example, the antler is prepared by preparing one or more antlers selected from the group consisting of red deer, Japanese deer, wapiti deer, and reindeer. can be cut to a length of 10 to 20 cm.

Next, it is possible to prepare a hot water extract from the green leaf from which the useful components of the green leaf are extracted by mixing the chopped green leaf with purified water and heating it.

In the above step, after mixing in a weight ratio of 3,000 to 4,000 parts by weight of purified water with respect to 100 parts by weight of the whole minced antler, heated at a temperature of 80 to 90 ° C. for 5 to 15 hours to prepare a hot water extract from which useful components of antlers are extracted can do.

Next, the antler hot water extract can be filtered to remove solids, and then concentrated to prepare an antler horn concentrate.

In the above step, the antler horn hot water extract is filtered with a known filter to prepare an antler filtrate, and the deer antler filtrate is heated at a temperature of 70 to 80° C. to remove moisture, thereby preparing a gel antler concentrate.

Subsequently, the antler concentrate is mixed with the first enzyme to perform primary enzymatic decomposition, and then heated to inactivate the first enzyme, and the first enzymatically decomposed antler concentrate may be cooled.

In the above step, as the first enzyme, a commercially available proteolytic enzyme may be used. For example, as the first enzyme, the endoprotease Alcalase, Nutraase, Protamex, and Novo. Any one or more proteolytic enzymes selected from the group consisting of pro and formea may be used.

Also, in the above step, after mixing the antler concentrate with the first enzyme, it is first enzymatically decomposed at a temperature of 40 to 50 ° C. After inactivation of the first enzyme by heating, the first enzymatically decomposed heated antler concentrate may be cooled by storing it at a temperature of 20 to 40°C.

Next, the first decomposed antler concentrate is mixed with a second enzyme for secondary enzymatic decomposition, and then heated to inactivate the second enzyme, and the second enzymatically decomposed antler concentrate may be cooled.

In the above step, as the second enzyme, a commercially available proteolytic enzyme may be used. For example, as the second enzyme, any one selected from exopeptidase flavourzyme or protana More than one proteolytic enzyme may be used.

In addition, in the above step, the first decomposed antler concentrate is mixed with a second enzyme, followed by secondary enzymatic decomposition at a temperature of 440 to 50° C., and 20 The second enzyme is inactivated by heating to 40 minutes, and the cooling may be performed by storing the second enzymatically decomposed heated antler concentrate at a temperature of 20 to 40 °C.

Next, by drying the second enzymatically decomposed antler horn concentrate, it is possible to prepare antler collagen.

In the above step, by drying the second enzymatically decomposed deer antler concentrate to remove moisture, low-molecular antler collagen can be produced. Invar, detailed description thereof will be omitted for convenience of description and clarity of the technical idea of the present invention.

On the other hand, the health supplement composition for improving bronchial function according to the present invention, in addition to the composition consisting of the red pepper cod extract, ginseng wormwood extract, seaweed extract, green tea extract, egg yolk oil, and antler collagen, hoesaeng jasmine extract, green lipped mussel oil and banyan tree extract may further include.

In addition, the health supplement composition for improving bronchial function according to the present invention contains 3 to 7 parts by weight of the red pepper root extract, 2 to 4 parts by weight of the Injin mugwort extract, 1 to 5 parts by weight of the algae extract, 2 to 4 parts by weight of the green tea extract, and egg yolk oil. 1 to 3 parts by weight and 0.5 to 1.5 parts by weight of antlers collagen, 0.5 to 0.9 parts by weight of the hoesaengi capricorn extract, 0.3 to 0.7 parts by weight of green lipped mussel oil, and 0.1 to 0.5 parts by weight of the Sangha mushroom extract. have.

The sargassum horneri extract can be extracted from sargassum horneri, and is biologically classified as a plant-family brown algae plant group, brown algae, and is mainly distributed in the western part of the North Pacific Ocean, and is mainly distributed in the lower intertidal zone. grows in In the domestic coast, it is easily observed that a part of the body floats on the water surface at low tide, and the most common marine forest species or young individuals are edible.

The sargassum horneri is yellowish-brown, and the stem from the button-shaped root is elongated as a single strand or sometimes bifurcated and branched from the upper part. Currently, atopy inhibitory effect, anti-inflammatory effect, and heavy metal adsorption effect have been reported, and antioxidant, adipocyte production inhibitory effect, cancer cell proliferation inhibitory effect, and anti-blood clotting effect have been reported through screening experiments.

The green-lipped mussel oil is manufactured using green-lipped mussels (Perna Canaliculus), which is a kind of mussels that inhabit the coast of New Zealand and is called green-lipped mussel, and its scientific name is Perna. Canaliculus.

It has been reported that the green-lipped mussel contains omega unsaturated fatty acids related to antioxidant and anti-inflammatory properties, that is, a unique component that cannot be seen in mussels that are native to other regions, because the green-lipped mussel feeds mainly on plankton that has evolved to resist intense ultraviolet rays.

In addition, the unsaturated fatty acids found in the green lipped mussel have been known through clinical trials to have a 200-fold stronger anti-inflammatory effect than fish oil, linseed oil, evening primrose oil, and the like containing omega-3 fatty acids. Omega-3 fatty acids have been found to inhibit the occurrence of inflammatory diseases such as atherosclerosis and rheumatoid arthritis by interfering with the production of inflammatory mediators made from omega-6 fatty acids by competing with omega-6 linoleic acid. there is a bar

As the green-lipped mussel oil, green-lipped mussel oil prepared by the following method may be used.

First, the skin of the green-lipped mussel may be removed, and the green-lipped mussel from which the skin has been removed may be washed.

In the above step, the green-lipped mussel from which the skin has been removed can be washed with a sodium bicarbonate solution having a temperature of 10 to 20° C. and a concentration range of 1 to 3 (w/w)%, and the sodium bicarbonate (NaHCO3) is a food product. It is also used as an additive, is non-toxic, and may have functions such as penetration, diffusion, and expansion.

Next, the washed green-lipped mussels may be dried.

Drying of the washed green-lipped mussel may consist of a first drying and a second drying, wherein the first drying is performed by drying the washed green-lipped mussel at a temperature of 70 to 80° C. for 3 to 5 hours. Residual moisture in green-lipped mussels can be removed primarily.

In addition, in the second drying, the first dried green-lipped mussel may be dried with hot air, and the second drying is performed by applying hot air to the first dried green-lipped mussel at a temperature of 110 to 130° C. for 1 to 3 hours. This can be done by adding

Next, the second dried green-lipped mussel can be pulverized and then aged to prepare an aged green-lipped mussel powder.

In the above step, the grinding of the second dried green-lipped mussel is pulverized by pulverizing the second dried green-lipped mussel to a particle size of 5 to 10 mm, and the aging of the powdered green-lipped mussel is the powdered green mussel. It can be carried out by storing the lipped mussels at a temperature of 5 to 10° C. for 3 to 7 hours.

Then, the green-lipped mussel aged powder can be enzymatically decomposed by mixing the enzyme and acid and storing the mixture.

That is, in the above step, the enzyme and acid are mixed in the aged powder of green lipped mussel, and then the aged powder of green lipped mussel in which the enzyme and acid are mixed is stored at a temperature of 36 to 38° C. for 20 to 30 hours, thereby enzymatic decomposition. .

In the above step, known commercially available protease (Protease), chitinase (chitinase), pectinase (Pectinase) and xylanase (Xylanase) are used as the enzyme, and citric acid and ascortic acid are used as the acid. Binic acid may be used, specifically, 0.01 to 0.03 parts by weight of the protease, 0.03 to 0.05 parts by weight of the chitinase, and pectinase based on 100 parts by weight of the whole aged powder of green lipped mussel. 0.005 to 0.009 parts by weight, 0.008 to 0.012 parts by weight of Xylanase, 0.01 to 0.1 parts by weight of citric acid, and 0.01 to 0.1 parts by weight of ascorbic acid may be mixed in a weight ratio.

Next, the fermented green-lipped mussel fermented product may be prepared by mixing the enzyme-decomposed green-lipped mussel aged powder with a fermentation broth consisting of lactic acid bacteria and a culture solution.

In the above step, after mixing in a weight ratio of 1 to 3 parts by weight of the lactic acid bacteria culture solution to 100 parts by weight of the enzymatically decomposed green-lipped mussel aged powder, fermentation may be performed at a temperature of 40 to 42° C. for 20 to 30 hours.

In addition, as the lactic acid bacteria in the above step, any one known lactic acid bacteria selected from the group consisting of Lactobacillales , Streptococcus thermophiles and Bifidobacterium longum may be used. , The culture solution consists of peptone, malt extract, sodium citrate, potassium phosphate dibasic, glucose and distilled water, and the culture solution is 15 to 25 parts by weight of peptone, 5 to 15 parts by weight of malt extract, 0.5 to 1.5 parts by weight of sodium citrate, 0.1 to 0.3 parts by weight of potassium dibasic phosphate, 1 to 3 parts by weight of glucose, and 100 to 200 parts by weight of distilled water may be mixed in a weight ratio.

In the culture medium, the peptone is a nitrogen source that supplies proteins and supplies proteins necessary for the growth of microorganisms, and the malt extract contains various minerals, vitamin B group, and amino acids to help microorganisms grow, Silver is a mineral that maintains an optimized state for the growth of microorganisms by adjusting the pH of the culture medium, and can supply nutrients, and the glucose can function as an important component for lactic acid bacteria to metabolize sugar to produce organic acids.

Next, the green-lipped mussel oil can be prepared by separating the oil using the fermented green-lipped mussel.

The configuration, such as separating the oil using the green-lipped mussel fermented product in the above step, is a known technique to those of ordinary skill in the art, and for convenience of explanation and clarity of the technical idea of the present invention, A detailed description thereof will be omitted.

The Sanghwang mushroom extract can be prepared from the Sanghwang mushroom. The Sanghwang mushroom is a very rare perennial mushroom of basidiomycetes that does not propagate well. The fruiting body is made of wood.

The shape of the Sanghwang mushroom is initially maintained in the form of a lump of mud, but after it has matured, it is also called Suseol (樹舌) because its tongue is sticking out of a tree stump.

And the anti-cancer activity of Sangha mushroom is because of the component called beta-glucan contained in the mushroom. Beta-glucan is a generic term for polysaccharides in which glucose is polymerized around a β-13 chemical bond. Many studies have been reported that beta-glucan stimulates humoral and cellular immunity in the human body to amplify the immune system and act as an immune modulator.

Many parts of the mechanism of the anticancer effect of beta-glucan are also known, and these beta-glucans activate the immune function of normal immune cells such as macrophages, natural killer cells, and T cells rather than directly attacking cancer cells. It is known to inhibit the proliferation and evaporation of cancer cells and promote the increase of immune cells.

In addition, it has resistance to viruses and acts on viral diseases such as flu, so if you look at people wearing Sanghwang mushrooms, you can see that they do not get the flu easily or get cured quickly even if they do catch it.

For example, the Sangha mushroom extract can be prepared by extracting the Sangha mushroom using various extraction methods such as hot water extraction, organic solvent extraction, ultrasonic extraction, supercritical extraction, etc. Since the configuration is a known technique, a detailed description thereof will be omitted for convenience of explanation and clarity of the technical idea of the present invention.

Hereinafter, examples of the health supplement for improving bronchial function according to the present invention will be described in more detail.

< Example 1 >

A health supplement was prepared by mixing a composition consisting of 5 parts by weight of red pepper root extract, 3 parts by weight of Injin mugwort extract, 3 parts by weight of algae extract, 3 parts by weight of seaweed extract, 2 parts by weight of egg yolk oil and 1 part by weight of antler collagen.

< Example 2 >

A health supplement was prepared using the same composition as in Example 1, and in Example 2, in addition to the above composition, 0.7 parts by weight of hoesaeng mother and half extract, 0.4 parts by weight of green lipped mussel oil, and 0.3 parts by weight of Sangha mushroom extract by weight. The composition was further mixed to prepare a health supplement.

< Comparative Example 1 >

A health supplement was prepared using the same composition as in Example 1, and in Comparative Example 1, a health supplement was prepared to include only red pepper root extract, green tea extract and nokal collagen.

< Comparative Example 2 >

A health supplement was prepared using the same composition as in Example 1, but in Comparative Example 2, a health supplement was prepared to include only the Injin mugwort extract, green tea extract, and egg yolk oil.

Experimental Example 1. Bronchoconstriction inhibition test (in vitro)

It was confirmed whether the health supplements prepared in Examples 1 and 2 and Comparative Examples 1 and 2 had bronchial contraction inhibitory activity (see Experimental Methods: Nature, 1977, 270, 256-257; Jpn J Pharmacol, 1996, 72, 1-8; Kor J Pharmacogn, 1999, 30[4], 377-383)

For this, sensitization was performed by intravenously injecting anti-ovalbumin anti-serum in a volume of 2 ml/kg to Hartely male guinea pigs (400-500 g, BGI, Korea). After 48 hours of sensitization, the guinea pigs were killed by blood loss and the bronchial tubes were removed. Then, after removing the other tissues attached to the bronchus in the tyrode liquid, a ring-shaped incision was made to include 2-3 cartilages. After the incision, the cartilage part of the ring was incised while preserving the bronchial muscle, the threads were connected on both sides, and then suspended in an organ bath. After stabilization for a certain period of time, 10㎍/㎖ of carbachol was administered. to induce maximal contraction, and the bronchi were washed and stabilized with tyrode solution. After that, 5 μm of indomethacin was added, and after 1 minute each extract (lyophilized and treated to 0.3 mg/ml) was added, and after 5 minutes, 20 μg/ml of ovalbumin was added to prevent contraction. induced. The bronchial contraction inhibition rate (%) was calculated by comparing the contractions caused by carbachol and egg albumin, and the results are shown in [Table 1]. The bronchial contraction and relaxation was measured using a force transducer (WPI) and a physiological activity measuring instrument (powerlab 8/30, ADInstument).

division Bronchial contraction inhibition rate (%) Example 1 80.85 Example 2 83.23 Comparative Example 1 42.28 Comparative Example 2 43.01

Referring to [Table 1], it was confirmed that the health supplements according to Examples 1 and 2 were significantly superior to the health supplements according to Comparative Examples 1 and 2 in inhibiting bronchial contraction activity.

Experimental Example 2. Airway constriction inhibition test (in vivo)

It was confirmed whether the health supplements prepared in Examples 1 and 2 and Comparative Examples 1 and 2 had airway constriction inhibitory activity (Experimental method-Respiratory volume measurement confirmation: European Journal of Pharmacology, 2000, 403, 169-179; Br J Pharmac, 1983, 78, 67-74)

To this end, Hartely male guinea pigs (400-450 g, SLC, Japan) were sensitized by intravenous injection of anti-ovalbumin antiserum at a volume of 15 ml/kg. After 48 hours of sensitization, the lyophilized product (400 mg/kg) of each extract was orally administered to guinea pigs. 30 minutes after oral administration, pyrilamine maleate (0.5 mg/kg) and proranolol (propranolol, 0.05 mg/kg) were subcutaneously injected and pre-treated, and a platismometer to measure various respiratory indicators of guinea pigs (plethysmometer) was placed in a double-chamber plethysmograph box (plethysmograph box type 855, HSE, German) equipped with a basic airway resistance value (RxV) was measured. After 30 minutes of pretreatment, 1% egg albumin was made into an aerosol using high-pressure compressed air, sprayed for 2 minutes, and the air containing albumin in the chamber was flowed out for 30 seconds. After that, airway resistance was measured in a plethysmometer box for 15 minutes, and airway resistance was calculated as AUC (area under the curve). indicated as

division Airway contraction inhibition rate (%) Example 1 47.21 Example 2 48.56 Comparative Example 1 23.15 Comparative Example 2 22.58

Referring to [Table 2], it was confirmed that the health supplements according to Examples 1 and 2 had significantly superior airway constriction inhibitory activity compared to the health supplements according to Comparative Examples 1 and 2.

Experimental Example 3. Bronchial inflammation inhibition test

To determine whether the dietary supplements prepared in Examples 1 and 2 and Comparative Examples 1 and 2 have an inhibitory effect on lung bronchial inflammation in asthma and chronic obstructive pulmonary disease models, lung bronchial induced by exposing antigens to sensitized mice was confirmed (Pharmacological Research, 2010, 61, 288-297; Biochemical Pharmacology, 2010, 79, 888-896).

For this purpose, BALB / c female mouse (65 weeks old, SLC, Japan) with 10㎍ of ovalbumin (Ovalbumin, OVA, sigma) and aluminum hydroxide (4mg, aluminum hydroxide, Alum, Pierce) 1:1 mixture solution 0.2 ml was administered intraperitoneally on days 0, 7 and 14 to sensitize. After 8 and 10 days from the last sensitization day, 10% egg albumin was made into an aerosol using high-pressure compressed air and sprayed for 50 minutes to induce airway inflammation. Administration of each test substance (400 mg/kg of lyophilisate of each extract, bid) was challenged by oral administration twice a day in the morning and afternoon from the 21st to the 23rd after the first sensitization. After 24 hours of the last challenge, bronchoalveolar lavage was collected using 15 ml of phosphate buffer solution (pH 7.2). The number of white blood cells in the washing solution was counted using a hematology analyzer (Drew Scientific Inc, HEMAVET HV950FS, M-950HV). The results are shown in [Table 3] below by calculating the pulmonary bronchial inflammation inhibition rate (%) based on the number of leukocytes in the negative control group (1% CMC [carboxymethyl cellulose] administered group).

division Pulmonary bronchial inflammation inhibition rate (%) Example 1 21.09 Example 2 21.86 Comparative Example 1 7.21 Comparative Example 2 7.59

Referring to [Table 3], it was confirmed that the health supplements according to Examples 1 and 2 were significantly superior to the health supplements according to Comparative Examples 1 and 2 in inhibiting bronchial inflammation.

Experimental Example 4. Toxicity Test

This experiment was conducted to investigate the toxicity of the health supplements of Examples 1 and 2 to the animal body acutely (within 24 hours) when ingested in excess in a short period of time, and to determine the mortality rate. Thirty ICR mouse strains, which are general mice, were assigned to the control group and the experimental group by 10 mice each. In the control group, only PEG-400/tween-80/EtOH (8/1/1, v/v/v) was administered, and the experimental group received the health supplements of Examples 1 and 2 of the present invention with the PEG-400/tween- It was dissolved in 80/EtOH (8/1/1, v/v/v) and administered orally, respectively. As a result of examining each mortality rate 24 hours after administration, it was confirmed that all mice survived in the control group and the experimental group administered with the dietary supplements of Examples 1 and 2 of the present invention at a concentration of 2 g/kg/day.

Experimental Example 5. Evaluation of bronchial function improvement effect

Targeted at private institute instructors and salespeople, who use their neck a lot, 60 people in their 20s and 40s who usually have bronchial symptoms such as cough and sputum (including acute bronchitis, chronic bronchitis, infectious bronchitis, catarrhal bronchitis, purulent bronchitis, (including patients suffering from obstructive bronchitis, ulcerative bronchitis, infiltrative bronchitis, asthma, and bronchial tuberculosis), 20 mg each of the dietary supplements according to Examples 1 and 2 and Comparative Examples 1 and 2 once a day were consumed each.

And one month later, the improvement effect was evaluated on the degree of improvement of the subject's cough, sputum, hoarseness, and bronchial itchiness, and the results are shown in [Table 5] below.

A 9-point scoring method was used for the score and evaluation criteria, and is shown in [Table 4] below.

score Evaluation standard 9 complete improvement 7 significant improvement 5 moderate improvement 3 slight improvement One no improvement

division cough sputum choking bronchial itching before intake 0 0 0 0 Example 1 8.1 8.0 8.2 8.1 Example 2 8.3 8.2 8.2 8.2 Comparative Example 1 4.5 4.7 4.6 4.6 Comparative Example 2 4.6 4.9 4.7 4.7

Referring to [Table 5], it can be seen that the cough, sputum, hoarseness, and bronchial itchiness of the subjects who consumed the dietary supplement according to Examples 1 and 2 were remarkably improved.

As described above, a preferred embodiment of the present invention has been described, but those of ordinary skill in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential features thereof. will be able Therefore, it should be understood that the embodiment described above is illustrative in all respects and not restrictive.

Claims (5)

A health supplement composition for improving bronchial function, characterized in that it contains red pepper cod extract, wormwood extract, algae extract, green tea extract, egg yolk oil and antler collagen.
The method of claim 1,
In a weight ratio of 3 to 7 parts by weight of the red pepper root extract, 2 to 4 parts by weight of the Injin mugwort extract, 1 to 5 parts by weight of the algae extract, 2 to 4 parts by weight of the seaweed extract, 1 to 3 parts by weight of egg yolk oil, and 0.5 to 1.5 parts by weight of antler collagen. A health supplement composition for improving bronchial function, characterized in that it is included.
3. The method of claim 2,
In addition to the composition consisting of the red pepper cod extract, Injin mugwort extract, ragwort extract, green tea extract, egg yolk oil and antler collagen, it further comprises an extract of hoesaengi capsicum extract, green-lipped mussel oil, and Sanghwang mushroom extract. Health aid for improving bronchial function food composition.
4. The method of claim 3,
3 to 7 parts by weight of the red pepper root extract, 2 to 4 parts by weight of the Injin mugwort extract, 1 to 5 parts by weight of the algae extract, 2 to 4 parts by weight of the seaweed extract, 1 to 3 parts by weight of egg yolk oil and 0.5 to 1.5 parts by weight of the antler collagen, A health supplement composition for improving bronchial function, characterized in that it further comprises 0.5 to 0.9 parts by weight of hoesaengi mother and half extract, 0.3 to 0.7 parts by weight of green lipped mussel oil, and 0.1 to 0.5 parts by weight of Sanghwang mushroom extract.
A health supplement for improving bronchial function, characterized in that it is prepared by including the composition of any one of claims 1 to 4.