KR101688542B1 - Functional Food Composition Comprising Red Ginseng Mixed Composition and Manufacturing Method Thereof - Google Patents

Abstract

The present invention relates to a functional food composition comprising a fermented red ginseng mixed composition including red ginseng powder and fermented mixed powder. The fermented and mixed powder comprises Lepidium meyenii, Turnera diffusa, Willd, seeds of a pumpkin, soybean, ginger, cornus fruit, Lycii fructus, Bombyx mori and Poria cocos Wolf. The functional food composition comprising the fermented red ginseng mixed composition according to the present invention comprises the steps of: pre-treating raw materials of fermenter and mixed powder consisting of Lepidium meyenii, Turnera diffusa, Willd, seeds of a pumpkin, soybean, ginger, cornus fruit, Lycii fructus, Bombyx mori and Poria cocos Wolf; producing fermented and mixed powder by mixing and aging the pre-treated raw materials with fermenting microorganism; producing fermented red ginseng mixed powder by mixing the fermented and mixed powder and red ginseng powder; producing a mixed fermentation extraction concentrate by extracting a part of the fermented red ginseng mixed composition and concentrating the same; and producing a red ginseng mixed composition by spraying the mixed fermentation extraction concentrate into the fermented red ginseng mixed powder and drying the same.

Description

{Functional Food Composition Comprising Red Ginseng Mixed Composition and Manufacturing Method Thereof}

The present invention relates to a functional food composition comprising a red ginseng fermented mixed composition comprising red ginseng powder and fermented mixed powder, and more particularly, to a functional food composition containing red ginseng fermented mixed composition of red ginseng powder and fermented mixed powder, Active oxygen scavenging, alcoholic erectile dysfunction, sexual function improvement, and a method for producing the functional food composition.

In recent years, with the increase in the average life span of human beings due to the advancement of medicine, the demand for improvement in the quality of life guaranteed by health has been higher than ever. As a method for improving the individual's health index, Interest is growing.

Due to this tendency, the development of health functional foods using natural products including plant and animal products such as herbal medicines and herb plants with functional properties is being developed all over the world.

In general, natural products are less toxic and adverse in vivo than synthetic products, and they are known to prevent diseases by exhibiting various physiological activities such as prevention of lifestyle diseases, antioxidation, anti-aging and anti-cancer effect.

On the other hand, sexual dysfunction can be a serious social problem as well as loss of self-confidence, powerlessness, and negative interpersonal relationships. In particular, erectile dysfunction (ED) is the most common and common sexual dysfunction among males in the world, not only in Korea but also in male patients. Considering the number of patients, the cost of treatment and the psychological shock caused by them, (NIH Consensus Conference, NIH Consensus Development Panel on impotence, JAMA, 270: 83-90, 1993).

Recently, other oral ED drugs including Viagra have been developed and used, but ED problems are not completely solved. In other words, these medicines are temporary relieving agents of erectile dysfunction, and they are not able to cure the ED fundamentally, and it is reported that the side effects due to long-term use as well as the cost problems are serious. Therefore, the development of a sexual enhancement agent using natural substances that are safer and more functional than synthetic ED agents such as Viagra is not only helpful in improving health and ED, but also in reducing medical costs It is expected that the scope of the beneficiary will be expanded through the reduction of side effects.

Recently, it has been reported that the ED effect is mediated through the regulation of the physiological functions of phytochemicals and sexual function improvement by natural substances such as medicinal plants and herbs (MacKay D., Nutrients and botanicals for erectile dysfunction: Examining the evidence. Int. J. Androl. 23: 82-84, 2000), among the natural products, in particular, Of the crucifix named Maca Lepidium Meyenii is known as Ginseng of the Andes because it has a stamina and sexual enhancement efficacy (Rea J. Andean roots.In: Neglected crops: 1492 from a different perspective.Hernando JE and Seon J., eds. 165-179, Plant production and protection series No. 26, FAO, Rome, Italy, 1994).

Maca is a traditional medicinal plant in the Andes region of Peru. It grows at an altitude of 4,000 ~ 4,500m, and its nutrients, calcium, iron and other minerals are abundant in its roots, and indigenous peoples have been used for fatigue, anemia, tuberculosis and infertility. Macaenes and macamides with pharmacological activity were isolated from maca (Ganzera M., Zhao J., Muhammad H, Khan IA. Chemical profiling and standardization of Lepidium meyenii (Maca) by reversed phase high performance liquid chromatography. Chem. Pharm. Bull. 50: 988-991,2002; Piacente S., Carbone V., Plaza A., Zampelli A., Pizza C., Investigation of the tuber constituents of maca ( Lepidium meyenii Walp.). J. Agri. Food. Chem. 50: 5621-5625, 2002) The benzyl glucosinolate of maca has been reported to be degraded into isothiocyanate, indole-3-carbinol, which has chemoprotective activity by intestinal microorganisms, to prevent prostate cancer and prostate enlargement (Gonzales GF, Miranda S., Nieto J., Fernandez G., Yucra S., Rubio J., Yi P., Gasco M., Red maca reduced prostate size in rats.Reprod. Biol. Endocrinol.3: 5-32, 2005).

When the maca were administered to rats, the spermatogenesis was promoted in male rats (Gonzales GF, Ruiz A., Gonzales C., Villegas L., Cordova A .. Effects of Lepidium metenii (maca) roos on spermatogenesis of male rats. Asian J. Androl. 3: 231-233,2001; Gonzales C., Rubio J., Gasco M., Nieto J., Yucra S., Gonzales GF, Effect of short-term and long-term treatments with three ecotypes of Lepidium meyenii (Maca) on permatogenesis in rats. J. Ethnopharmacol. In Press, 2005), sexual enhancement (Cicero AFG, Bandieri E., Arletti R., Lepidium Walpel improves sexual behavior in male rats idependently from its action on spontaneous locomotor activity. J. Ethnopharmacol. (Bustos-Obregon E., Yucra S., Gonzales GF, and Lepidium meyenii (Maca)) induced spermatogenic damage by a single dose of malathion (Riziz-Luna AC, Salazar S., Aspajo NJ, Rubio J., Gasco M., Gonzales GF, Lepidium meyenii (Maca) increases litter size in normal adult female mice. Reprod. Biol. Endocrinol. 3: 3-16, 2005). In addition, it has been reported that sexual function enhancement, spermatogenesis and sperm motility are increased in adult men, and it is attracting attention as a natural product related to sexual function improvement (Gonzales GF, Cordova A., Vega K., Chung A., Villena A., Gonez C., Castillo S., Effect of Lepidium meyenii (Maca) on testosterone levels in adult healthy men. Androl. 34: 367-372, 2002; Gonzales GF, Cordova A., Gonzales C., Chung A., Vega K., Villena A., Lepidium meyenii (Maca) improved semen parameters in adult men. Asian J. Androl. 3: 301-303, 2003).

On the other hand, Panax ginseng, which is widely known at home and abroad among the herbal medicines that have not the effect of caloric enhancement or treatment, has a physiological function controlling effect. It is a traditional Korean health food. It is used for functional foods and medicines useful for strengthening physical strength and restoring fatigue and fatigue (Bucci LR, Selected herbals and human exercise performance, Am J Clinc Nutr. 72: 624S-636, 2000). Ginseng has various pharmacological actions such as stimulation of central nervous system, synthesis of tissue proteins, enhancement of immunity, strengthening of antioxidant power (Bahrke MS, Morgan WP. Evaluation of the ergogenic properties of ginseng. Sports Med. 18: 229-248, 1994 Yasuda T., Yu Y., Zheng P., Kawabata T., Ma Y., Okada S., < RTI ID = 0.0 > Ginseng extract scavenges hydroxyl radicals and protects unsaturated fatty acids from decomposition caused by iron-mediated lipid peroxidation. Free Radic Biol. Med. 20: 145-150, 1996) (Chung NJ, Some physiological and biochemical aspects of saponin fraction of Panax Ginseng CA Meyer. Kor J. Ginseng Sci. 14: 143-156, 1990; Joo CN , Several physiological functions and metabolism of ginsenosides in t he animal body. Kor J. Ginseng Sci. 17: 250-286, 1993). In addition, ginseng has been reported to have sexual or sexual restorative effects (Murphy L. L., Lee T. J., Ginseng, sex behavior, and nitric oxide. Annals NY Acad Sci. 962: 372-377,

In particular, red ginseng processed from Korean ginseng has a high content of crude saponin and major ginsenosides (Lee JW, Lee SK, Do JH, Comparison of the content of saponin and mineral component in Korean red ginseng and other red ginseng, J. Ginseng Res. 26, 196-201, 2002), it has been reported that ED has a significant effect on the improvement of ED in patients with erectile dysfunction (Hong B., Ji YH, Hong JH, Nam KY, Ahn TY, A double-blind crossover study, evaluating the efficacy of Korean red ginseng in patients with erectile dysfunction: a preliminary report, J. Urol., 168: 2070-2073, 2002).

In a recent study, red ginseng increased the number of sperm and increased sexual function in patients with sexual dysfunction (Salvati G., Genovesi G., Marcellini L., Paolini P., De Nuccio I., Pepe M., Re M., Effects of Panax Ginsengca (Yang WJ, Lee BJ, Nam SY, Yang DC, Yun YW, Modulating effects of Korean ginseng saponins on ovarian function in immature rats. Bio-Pharm Bull. 26: 1574-1580, 2003), Prevention of Tissue Cell Oxidation in Pregnant Rats (Song YB, Kwak YS, Park KH, Chang SK, Rats, J. Ginseng Res. 26: 139-144, 2002) and sperm function recovery in toxic exposed rats (Hwang SY, Kim WJ, Wee JJ, Choi JS, Kim SK, Panax ginseng improves survival and sperm quality in guinea pigs exposed to 2,3,7,8-tetrachlorodi benzo-p-dioxin. BJU Int. 94: 663-668, 2004) The.

In Korean Patent Registration No. 10-0538717, red ginseng concentrate and ginseng extract, bombyx mugwort, omija, tosaja, and casualty ginseng have obtained the effect of sexual function improvement. In Korean Patent Registration No. 10-1385657, red ginseng extract In the Korean Patent Registration No. 10-0850503, there is disclosed a composition for preventing or treating erectile dysfunction of male erectile dysfunction, wherein the composition comprises mung bean oil, omija, koshiogapi, bokbunja, ginseng extract, , Garlic, broiler, tosaja, japonica, and ginseng extract.

Damiana, Turnera diffusa , Willd) is a plant native to South America and the Midwest, which can enhance the sexual function of males and females, increase orgasm, and is a proven herb widely used in oriental medicine. It is a yellow-blooming shrub that grows in the wild or cultivated, and leaves are harvested when blooming in the summer.

It has been used as a tonic for thousands of years in Latin American civilization, and has been used as a nervous stabilizer by making incense sticks (incense sticks). In recent years, effective substances of flavon glycoside materials and flavonoids having a novel structure have been identified. In addition to enhancing sexual function, functions such as improvement of dizziness, anti-anxiety, and anti-depression have been reported It is also used as a circulatory reliever for relieving asthma, relieving cold symptoms, relieving headache due to menstruation, and relieving constipation.

Pumpkin seeds contain vitamins B, C and E and urease, which is an enzyme, and about 40 ~ 50% of oil. Linoleic acid, oleic acid, palmitic acid and stearic acid are known as main components (Procida G., Stancher B., Cateni F. , Zacchigna M., Chemical composition and functional characterization of commercial pumpkin seed oil, J. Sci. Food Agric. 93: 1035-1041, 2012). Most of them are composed of unsaturated fatty acids linoleic acid and oleic acid. Linoleic acid is well known for its antiinflammatory action, and oleic acid is known to be effective in wound healing, immunity and inflammatory diseases and cancer.

Soybean (Soybean, Glycine max L) soy protein, and a peptide, oligosaccharide, phospholipid, isoflavones, saponins, vitamins and minerals and is contained, the physiologically active ingredient of saponin, a flavonoid, such as the large amount. Soybean protein reduces blood cholesterol and blood lipoprotein (LDL) concentration, thereby preventing atherosclerosis and heart disease. Soybean oligosaccharide promotes propagation of enterobacteria in the intestines. Soybean fiber promotes cholesterol excretion, It is known that there are effects such as increase of blood sugar and suppression of insulin secretion.

Soybean saponin has the functions of inhibition of obesity, reduction of blood cholesterol, anticancer, inhibition of human immunodeficiency virus (HIV) proliferation, anti-aging, etc. The other soybean components are skin cancer, bladder cancer, lung cancer, colon cancer, pancreatic cancer , Esophageal cancer, prostate pressure, liver pressure, and gastric cancer.

Ginger (Zingiber officinale Roscoe) is a perennial crop belonging to the ginger family originating in tropical Asia. It has been used as a stomach medicine to strengthen the stomach in a herb and is said to be effective in digestion, vomiting and pain. Ginger contains volatile fragrance components such as zingiberene and γ-cardinen, and essential oils such as zingiberol and zingiberene. Especially, oleoresin, which is a unique taste of ginger, exists as a mixture of several homologs. Among them, 6-gingerol and 6-shogaol are attracting attention as a health functional food material because they have antibacterial action, salt action, serum cholesterol lowering effect and antioxidant action (Sheo HJ, The antibacterial action of garlic, onion, ginger and red pepper juice, J. Kor. Soc. Food Sci. Nutr. 28: 94-99, 1999; K., Lee IS, Comparison of gingerols in various fractions and the antioxidant effects of supercritical fluid extracts from ginger, Kor J. Food Sci. Technol., 43: 469-474, 2011).

Cordyceps is a kind of mushroom which grows on insects as a host and forms spores on the larvae when the temperature and humidity become high in the summer when it becomes parasitic to various kinds of insects in winter. According to Chinese ancient books, Cordyceps is not only effective in cancer, liver disease, lung disease, brain disease, cardiovascular disease, heart disease, diabetes, etc., but it also has pulmonary tuberculosis, elderly debilitating chronic tuberculosis anemia, And the like.

Recently, it has been reported to be effective as an anticancer and antidote to drug poisoning. Immune enhancement, antibiotic effect, antidiabetic effect, hyperlipidemia improving action and platelet aggregation inhibiting action have been reported (Lee HH, Cho HS, The effect of Cordyceps militaris on adaptive immune responses in DBA2 mice with immunization with influenza vaccine. Uakhak Hoeji. 59: 1-5, 2015).

Cornus syrup is a late-fall and early-winter fruit that turns red in the perilla ( Cornus officianalis ) and extracts the seeds from the seeds of the dried fruits. In the Oriental medicine, corn oil is tasted, its properties are slightly warm, and it is reported that it has liver, nervousness, diuretic action, hypotensive action, action to help digestion of protein, anticancer and antibacterial action. These compounds include various organic acids and iridoid glycosides such as morronoside, loganin, and sweroside, and tannins. They reduce oxidative stress by reducing active oxygen species, inhibit the expression of lipid synthesis-related molecules, and act on lipid metabolism (Kwak, IS, Kim, MM, Chung, KT). Antioxidative activity of Cornus is known to be effective in improving cognitive function Extracts of officianalis obtained by four different extraction techniques. J. Life Sci. 22: 1507-1514, 2012).

Gugija ( Lycii fructus ) was isolated from Lycium chinensis Miller) contains a large amount of functional ingredients such as carotenoids, choline, melissic acid, zeaxanthin, physalin, betaine, β-sitosterol, vitamin B1, rutin and unsaturated fatty acids. Antioxidant effect, antibacterial effect, , blood pressure lowering and anti-diabetic effects, it is known the efficacy of such immune enhancing effect (Lee KA, Antioxidative and anti-diabetes activity, and free amino acid and mineral contents of beverage with Gugija (Lycii fructus ) extracts. Korean J. Food Cook Sci. 31: 207-213, 2015).

Silkworm ( Bombyx mori ) is a larva of the silkworm moth (Bombycidae), which is reported to be effective in treating children's parasites and eliminating parasites. According to a reported study, the hypoglycemic effect (Chung SH, Ryu JH, Kim IJ, Blood glucose lowering of silkworm. J. KyungHee Pharm. 24: 95-100, 1996; Pyu KS, Lee HS, Chung SH, Kang PD, An activity of lowering blood glucose levels according to preparatory conditions of silkworm power. (Mucus secretion in rat tracheobronchial lumen), which has been shown to induce mucin secretion in bronchial tissues (Seric Sci., 39: 79-85, 1997) (Kang YK, Oh HS, Cho YH, Kim YJ, Han YG, Park JH, Park JH, , Nam SH, Effect of a silkworm extract on dopamine and monoamine oxidase-B activity in an MPTP-induced Parkinson's disease model. (Kim EJ, Kim SH, Kim SM, Dev elopement of a extract of extracts from Bombyx mori L., Morus alba L., Dioscoreae rhuzome and Inonotus obliquus. Kor. J. Food Preserv. 18: 844-852, 2011).

Poria cocos Wolf is the sclerot of the bacillus, which is a fungus belonging to the genus Pseudomonas aeruginosa, which parasitizes in the root of the ground after 4 to 5 years after cutting the pine tree. The main ingredients are starch and carbohydrate, water, fat, protein and minerals, and are widely used as herbal medicines and herbal medicines because of their effects on diuretic action, chronic gastritis and abdominal pain, hypoglycemic effect and malaise (Yun HJ, Cha HM, Kim SW, Shin WC, Effects of the extracts of hoelen on serum lipid profiles in mice, J. Kor. Soc. Food Sci. Nutr. 35: 1005-1009, 2006).

Octacosanol is a physiologically active substance which is contained in rice bran, syrup, sugar cane and so on in a very small amount. It is a physiologically active substance including muscle strength, muscle endurance, (Kato SK, Hasegawa S., Nagasawa J., Nagasaki A., Eguchi M., and Hamatani K.), which is an effective energy source for migratory birds. , Octacosanol affects lipid metabolism in rats fed on a high-fat diet (Br J. Nurt., 73: 433-441, 1995). Octacosanol intake has been reported to increase maximal oxygen uptake and reduce heart burden, enhance blood clotting and cardiac function, improve endurance, and prevent hyperlipidemia and heart disease (Mas R., Castano G., Illnait J., Fernandez L., Fernandez J., Aleman C., Pontigas V., Escay M., Effects of policosanol in patients with type II hyercholest-terolemia and additional coronary risk factors.Clin.Pharmacol. Ther. 65: 439- 447, 1999).

The study of the sexual function control effect of red ginseng, maca, and natural fermented products, which are natural and natural ginseng enhancing effect of sexual function and constitution improvement, is considered to be a very beneficial attempt for the development of a new health functional food. If this study is carried out, it can be verified whether or not the sexual function of the natural complex is increased. In the future, it will be possible to develop a natural viagra which is a male sexual enhancement agent.

In addition, the natural complexes are harmless to the human body in addition to the red ginseng and maca, which are different from the drugs which have temporal sexual function improvement and side effects, and can also provide physiological function control effect as a health functional food as well as sexual function improvement.

In addition, although it is considered that the application value is sufficient in terms of foodstuffs, there have been no researches on natural fermentation and aging and improvement of sexual function by fermentation containing a large amount of active ingredient. Therefore, Lt; RTI ID = 0.0 > of < / RTI >

On the other hand, fermentation is a next-generation technology in which research results on the effects of probiotics such as anticancer, cholesterol lowering, blood pressure lowering, blood clotting inhibition and diarrhea prevention are continuously announced by the food processing technology called bioconversion. The Codex affiliated with the World Health Organization has agreed to establish provisions for the use of probiotics using genetically modified microorganisms. Fermented microorganisms recognized as safe can be the core of the 21st century bio-industry. Is expected to become a new industrial field.

Bacillus, which is involved in traditional fermented food, is a aerobic bacterium that produces various hydrolytic enzymes and contributes greatly to soybean fermented foods. After the fermentation process, a physiologically active substance such as a protease, a carbohydrate hydrolase, a thrombolytic enzyme, a functional peptide and a polymer viscous substance is produced by Bacillus subtilis (Kwon HY, Kwon GS, Kwon CS, Sohn HY , Isolation of immuno-stimulating strain Bacillus pumilus jb-1 from Chungkook-jang and fermentation characteristics of JB-1. Kor J. Microbial. Biotechnol. 32: 291-296, 2004).

When fermented, the enzyme produced by Bacillus subtilis degrades the proteins and saccharides, resulting in a sticky viscous substance composed of poly-γ-glutamic acid (PGA) polymerized with levan form fructan and glutamic acid (Youn HK, Choi HS, , Hong JH, Antimicrobial activities of viscous substance form Chongkukjang fermented with different Bacillus spp., J. Fd. Safety 16: 188-193, 2001), these stomachs have an important influence on the quality characteristics of the fermented product.

In particular, PGA is a type of microbial polymer material that has physiological functions such as immunity enhancement and anticancer effects, and physiologically active substances produced by mainly decomposing carbohydrates and proteins have a decisive influence on the quality of products, (Lee TS, Choi JY, Volatile Flavor Components in Mash of Lactic Acid), which is known to exhibit various physiological activities depending on the production and increase of effective components such as polysaccharides, oligosaccharides, amino acids, peptides, organic acids and polyphenols Takju prepared by suing Aspergillus kawachii nuruks. Kor J. Food Sci. Technol., 37: 944-950, 2005).

The present inventors selected Bacillus licheniformis as an excellent strain by carrying out an antimutagenic activity, a viscous substance, a free amino acid content and a sensory test by using several strains isolated from Korean traditional cheonggukjang as a single strain (Lee, MY, Quality and functional characteristics of chungkukjang fermented by Bacillus sp. isolated from commercial products . MS Thesis. Catholic University of Daegu. p 1-62, 2005), is a bar to secure the technologies that increase the physiological activity of Bacillus licheniformis in fermentation products (Republic of Korea Patent Application 10 1204875).

Korean Patent Publication No. 10-0538717 Korean Patent Publication No. 10-1385657 Korean Patent Publication No. 10-0850503 Korean Patent Publication No. 10-1204875

MacKay D., Altern. Med. Rev. 9: 4-16, 2004. Adimoelja A., Int. J. Androl. 23: 82-84,2000. Gonzales G. F., Cordova A., Vega K., Chung A., Villena A., Gonez C., Castillo S., 34: 367-372, 2002. Gonzales G. F., Cordova A., Gonzales C., Chung A., Vega K., Villena A., Asian J. Androl. 3: 301-303, 2003. Murphy L. L., Lee T. J., Annals NY Acad Sci. 962: 372-377, 2002. Hong B., Ji Y. H., Hong J. H., Nam K. Y., Ahn T. Y., J. Urol. 168: 2070-2073,2002.

Disclosure of Invention Technical Problem [8] The present invention has been made to solve the above problems of the prior art, and it is an object of the present invention to provide a functional food composition containing red ginseng fermented mixed composition comprising red ginseng powder and fermented mixed powder, It is an object of the present invention to provide a functional food composition showing an effect of improvement.

In particular, the effect of improving sexual function was examined by examining the effects of oxidative damage on the testis and other organs of rats treated with 2-bromopropane (testosterone-inducing agent) for the mixed diet of Korean red ginseng and natural fermented powder It is an object of the present invention to provide a proven functional food composition.

In addition, long-term ethanol was administered to observe the changes in the activity of alcohol metabolism, ROS production system and elimination enzyme, and the index of liver tissue damage, thereby improving sexual function using natural fermented substances, preventing erectile dysfunction caused by alcohol, It is an object of the present invention to provide a health functional food composition for enhancing male health and sexual function which is improved by environmental hormones and oxidative stress.

The functional food composition of the present invention for solving the above problems is a functional food composition comprising a red ginseng fermented mixed composition comprising red ginseng powder and fermented mixed powder, wherein the fermented mixed powder is selected from the group consisting of maca, damiana, pumpkin seed, Ginger, Chinese caterpillar fungus, corn syrup, gugija, silkworm, and gypsum. In addition, the fermentation mixed powder may further comprise octacosanol.

In the present invention, the functional food composition comprising the red ginseng fermented mixed composition comprises a pretreatment step of pretreating a raw material of a fermentation mixed powder composed of maca, damiana, pumpkin seed, soybean ginger, cordyceps, acid fermented milk, gugija, silkworm, Mixing the pretreated raw material with a fermenting microorganism and aging the mixture to prepare a fermentation mixed powder; Mixing the fermentation mixed powder and the red ginseng powder to prepare a red ginseng fermentation mixed powder; Extracting and concentrating a part of the red ginseng fermentation mixed powder to prepare a concentrated fermentation extract concentrate; Mixing the red ginseng fermentation mixed powder with the mixed fermentation extraction concentrate and drying the mixture to prepare a red ginseng mixed composition.

The preparation of the fermented red ginseng composition comprises mixing the red ginseng fermented mixed powder with the mixed fermentation extract concentrate 10, 10, 10, 20, 30, 40, 50, And drying the mixture so that the water content becomes 5% or less.

The functional food composition according to the present invention exhibits the effects of hepatic detoxification, active oxygen scavenging, alcoholic erectile dysfunction and sexual function improvement through a functional food composition comprising red ginseng fermented mixed composition comprising red ginseng powder and fermented mixed powder.

In addition, by using Korean red ginseng and natural fermented powder as raw materials, the effect of alcohol metabolism, active oxygen species (ROS) production system and elimination enzyme activity on liver tissue damage and improvement of sexual function using natural fermented substance and alcohol It is possible to provide a health functional food composition for improving male health and sexual function, which is effective for prevention of erectile dysfunction, environmental hormone and oxidative stress.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a process diagram showing a process for producing a functional food composition according to an embodiment of the present invention; FIG.
FIG. 2 shows that when erectile dysfunction was induced by chronic ethanol administration during 4 weeks of rats fed with red ginseng extract (BR), red ginseng mixed composition extract (BRM), and red ginseng fermented mixed extract (BRFM) (A) and aspartate aminotransferase activity (b).
FIG. 3 shows that when erectile dysfunction was induced by chronic ethanol administration during 4 weeks of rats fed with red ginseng extract (BR), red ginseng mixed composition extract (BRM) and red ginseng fermented mixed extract (BRFM) (A), lipid peroxide content (b), and triglyceride content (c).

Hereinafter, the present invention will be described in more detail with reference to the drawings. The terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms and the inventor may appropriately define the concept of the term in order to best describe its invention It should be construed as meaning and concept consistent with the technical idea of the present invention.

The term "red ginseng fermented mixed composition" as used in the present invention refers to a mixture of red ginseng powder and fermented mixed powder, in which a mixed fermentation extract concentrate is added.

The term "fermented mixed powder" refers to a mixed powder prepared by mixing natural product powder with fermenting microorganism and fermenting it. The term "red ginseng powder" refers to pulverized red ginseng powder.

Also, the mixed fermentation extracted concentrate refers to an extract concentrate prepared by mixing red ginseng powder and fermented mixed powder and extracting and concentrating.

The term "red ginseng fermentation mixed powder" refers to a mixed powder prepared by mixing the fermentation mixed powder and the red ginseng powder.

The present invention relates to a functional food composition comprising a red ginseng fermented mixed composition comprising red ginseng powder and fermented mixed powder.

In the present invention, the fermented mixed powder is composed of maca, damiana, pumpkin seed, soybean, ginger, cordyceps, corn oil, goji, silkworm, and bamboo.

The above-mentioned maca, damiana, pumpkin seed, soybean, ginger, cordyceps, corn oil, gugija, silkworm, and bamboo are known to have various effects as well known in the art and can be used to improve sexual function.

In the present invention, the fermentation mixed powder can exhibit its effect when all of the above ten components are used. This is because, as described in the prior art, not only the effect of each of the above-mentioned 10 components but also the selection of various natural ingredients in order to obtain an elevated effect obtained by using them in combination, is different from a simple mixture of natural products.

It was found that when the one or two components out of the above 10 components are excluded, for example, except for maca, pumpkin seed, ginger, and ginger, the specific effects such as the sexual function improvement aimed at in the present invention can not be sufficiently obtained .

That is, each of the herbal medicine used as a raw material of the fermentation mixed powder is excellent in pharmaceutical efficacy, but provides a functional composition exhibiting the effects of liver detoxification, active oxygen scavenging, alcoholic erectile dysfunction and sexual function improvement, For the purpose of maximizing the efficacy of the fermentation process after mixing, it is used as a non-single complex composition.

In addition, the fermentation powder may further comprise octacosanol. Octacosanol extracted from sugarcane, which is a main energy source, is homogenized by a process of injection (liquid) or compounding (boiling) so that it can be uniformly contained in the manufacturing process of the red ginseng mixed composition.

The functional food composition according to the present invention can be produced by a process as shown in Fig.

That is, as shown in FIG. 1, a pretreatment step of pretreating raw materials of mixed fermentation powders composed of maca, damiana, pumpkin seed, soybean, ginger, cordyceps, corn oil, gougia, silkworm, and bamboo; Mixing the pretreated raw material with a fermentation microorganism and aging the mixture to prepare a mixed fermentation powder; Extracting and concentrating the mixed fermentation powder to prepare a concentrated fermentation extract concentrate; Mixing the mixed fermentation powder with the red ginseng powder, spraying the mixed fermentation extraction concentrate, and drying the mixture to prepare a red ginseng mixed powder.

The step of preparing the red ginseng mixed powder may include a step of mixing the powder obtained by mixing the red ginseng powder with the red ginseng powder, 10 parts by weight of the concentrated fermentation broth concentrate is sprayed and then dried so that the water content becomes 5% or less.

The fermented red ginseng mixed composition according to the present invention may be used in the form of a powder or as a solid preparation such as a tablet, a pill, a powder, a granule, a capsule, or a liquid preparation such as a suspension, a solution, an emulsion or a syrup It is possible. Therefore, in the present invention, the functional food composition can be produced by mixing the red ginseng fermented mixed composition as a main component and various additives according to the application.

Hereinafter, the functional food composition of the present invention and its preparation method will be described in detail by way of examples.

Red ginseng was purchased from Jinshan Agricultural Cooperative Federation and stored at 4 ℃. Maca, Damiana, pumpkin seed, soybean, ginger, cordyceps, corn oil, gougie, silkworm, bokyeong and octacosanol were purchased from farmers and raw materials. Bacillus licheniformis strains isolated from Korean traditional cheonggukjang were identified as Bacillus licheniformis strain (Lee MY, Quality and functional characteristics of chungkukjang fermented by Bacillus sp. Isolated from commercial products, MS Thesis, Catholic University of Daegu, pp. 1-62, 2005) Respectively. The reagents used for the efficacy analysis were the standard reagents.

Table 1 shows the name of the materials used, the site of use, and the content.

Name of material Scientific name and use area Content range (% by weight) Red ginseng  Red Ginseng, Panax ginseng C. A. Meyer Maca Maca , Lepidium meyenii 5 to 15 Damiana Damiana leaf, Turner diffusa , Willd. 10-20 Pumpkin seed Pumpkin seed, Cucurbita moschata 5 to 10 Big head Soybean, Glycine max L 5 to 15 ginger Zingiber officinale Roscoe 5 to 10 Cordyceps  Cordyceps militaris 10-20 Corn oil  Cornus officinalis 7 to 10 Gugija  Lycii fructus 7 to 10 silkworm  Bombyx mori 5 to 10 Boryeong Poria cocos Wolf 3 to 7 Octacosanol Octacosanol Proper amount

The content ranges of the respective materials are optimized to obtain the effect of improving the sexual function according to the combination of the components of the present invention. When the respective components are out of the above range and excessively or excessively small, Even when used as a powder component, it was found that effects such as sexual function improvement were not sufficiently manifested.

Using the ingredients listed in Table 1 above, the functional food composition of the present invention was prepared as follows.

The first step (pretreatment of raw material)

Each material was selected, washed, and then dried to give improved processing aptitude such as solubility and water absorbability. Each raw material is treated by steaming (steam, autoclave for 1 ~ 5 minutes, 95 ~ 100 ℃), where pressure is applied with appropriate high pressure steam to remove sterilization treatment and impurities that have not been removed, Changes have improved safety and processability (wettability, sedimentation, dispersibility and solubility).

Next, roasting (roasting, roasting machine; 120 ~ 220 ℃, 3 ~ 7min) was performed to inactivate the browning enzyme and rapid freeze-drying to minimize the loss of nutrients in order to improve taste, .

The dried material was first pulverized with a crusher and then crushed second by a fine crusher in order to increase the extraction of useful components and the promotion of fermentation, thereby obtaining a 120 mesh powder.

The second step (mixing and aging of the compounding and fermenting microorganisms)

11 wt% of maca, 17 wt% of Damiana, 7 wt% of pumpkin seeds, 11 wt% of soybeans, 7 wt% of ginger, 16 wt% of caterpillar fungus, 8 wt% of ginger, 8 wt% of silkworm, 9 wt% Were mixed equally and mixed at an appropriate ratio in order to improve the oxidative stress and induce sexual enhancement.

B. licheniformis , which has been proven to be useful, is inoculated uniformly into the powder, and a certain amount of distilled water (3% of the powder weight) is added thereto so that solid fermentation can be performed. Respectively. And the conditions for keeping the appropriate temperature and humidity suitable for the activity of the organism are provided to activate the growth of Bacillus subtilis in the fermentation condition and induce the fermentation of the material and the development of the useful strain through the low temperature aging.

After inoculation and molding, primary fermentation was carried out for 24 to 72 hours at a temperature of 35 to 40 ° C and a humidity of 70 to 90%, followed by low temperature aging for 7 to 14 days at a temperature of 10 to 25 ° C and a humidity of 45 to 55% . After the fermentation and aging, the components were pulverized so as to be finely pulverized with a fine grinder in order to increase extraction of useful components.

Fermented mixed powder was mixed with pretreated red ginseng powder to prepare red ginseng fermented mixed powder. The blending ratio is preferably 20 to 30% by weight of red ginseng and 70 to 80% by weight of fermented mixed powder. In this example, 25% by weight of red ginseng and 75% by weight of fermented mixed powder are mixed and homogenized.

The content ratio of red ginseng powder and fermented mixed powder is an important factor for achieving the effect from the food composition of the present invention. For example, when the red ginseng powder is out of the above range, the synergistic effect due to the fermentation mixed powder can not be obtained. On the contrary, when the red ginseng powder is out of the above range, the synergistic effect due to red ginseng can not be obtained. In addition, it is possible to achieve the desired effect of the present invention when the mixing ratio is homogenized by examining various mixing ratios such as the powder characteristics of each material, the improvement of the processability at the time of processing, Respectively.

Bacillus licheniformis , Bacillus pumilus , Bacillus sonorensis or Bacillus subtilis may be used as the Bacillus subtilis strain used for food, Preferably, Bacillus licheniformis or Bacillus subtilis is used.

In the case of Bacillus licheniformis , which is a fermentation strain used in the present invention, several strains isolated from Korean traditional cheonggukjang were used as a single strain. In addition, the antimutagenic activity, the viscous substance, the free amino acid content, and the sensory test can be performed to apply the strain selected as the excellent strain to the fermented product of the present invention, so that it can exert its effect on the enhancement of the body metabolism function.

The third step (extraction and concentration step)

Next, the extraction and concentration process for extracting useful components from the fermented red ginseng powder is carried out. In the case of extraction, functional substances and nutrients that are latent in the cells are extracted from the cells using a solvent, and the process proceeds to two steps of hot water extraction and alcohol extraction to extract and hydrolyze the enzyme hydrolyzate, which increases the peptide production of protein and natural mixture Thereby enabling efficient extraction of useful components.

The thick cell wall surrounding the useful ingredient is primarily decomposed by fermentation, and sequential extraction of hot water and alcohol facilitates penetration of the solvent in the tissue, thereby increasing the extraction yield of the useful substance.

Extraction of red ginseng mixed fermentation powder was performed as follows.

The primary hot water extraction was carried out by reflux extraction at 70 ° C for 6 hours with purified water of 10 times the raw material and then filtered. The secondary alcohol extraction was performed by adding 10 times of alcohol to the residue and refluxing for 3 hours at 60 ° C. , Filtered through a filter paper, and concentrated under reduced pressure to a solids content (Brix) of 15.

Step 4 (Mixture composition manufacturing, packaging and sterilization steps)

The preparation of fermented red ginseng mixed composition containing the extract as a method for increasing the content of the useful ingredient and improving the function of the raw material is carried out by mixing the fermented red ginseng powder and the fermented mixed powder (produced in Step 2) % By weight spraying uniformly into the sprayer and then drying at 40 < 0 > C.

This is dried to a moisture content of 5% or less. The water content should be adjusted in order to prevent the fermentation product from coming into contact with abnormal bacteria to prevent abnormal fermentation or decay. Normally, it is important to adjust the water content by drying because the microorganism can not grow at a water content of less than 6%. The mixed powder is packed in a pouch and then sterilized at an appropriate temperature to produce the final product.

To investigate the effect of the functional food composition of the present invention on the testes and other organs of rats treated with testosterone damage inducer, the effect of erythropoietin-induced liver detoxification, active oxygen scavenging enzyme activity, sexual function improvement Respectively.

Experiment 1. The food composition of the present invention is 2- bromopropane (testis damage inducer)  Effects of testicular and other organs on the degree of oxidative damage in treated rats

1) Preparation of experimental group and diet

In the experimental group, the control group (B), 2-bromopropane-treated group, red ginseng group (BR), 2-bromopropane group and red ginseng (BRM), 2-bromopropane administration, and 2% dietary group (BRFM) containing red ginseng and fermented mixed composition.

At this time, red ginseng extract, red ginseng plant composition extract solution and red ginseng fermentation plant mixture composition extract (each solid content Brix 20) were used as samples in which the concentration was doubled and the concentration was doubled.

The composition diet was fed for 4 weeks under the same conditions as in Table 2. The basic diet (AIN 76-A diet), minerals mixer and vitamin mixer were all prepared based on the composition of the United States Daughter Card (Teklad, USA).

Furtherance Experimental group NC B BR BRM BRFM Casein 200.0 200.0 197.0 197.0 197.0 cornstarch 150.0 150.0 134.0 134.0 134.0 Shrubber 500.0 500.0 500.0 500.0 500.0 Cellulose  50.0 50.0  49.0  49.0  49.0 Corn oil  50.0 50.0  50.0  50.0  50.0 AIN mineral blend  35.0 35.0  35.0  35.0  35.0 AIN vitamin blend  10.0 10.0  10.0  10.0  10.0 DL-methionine   3.0  3.0   3.0   3.0   3.0 Tartaric acid choline   2.0  2.0   2.0   2.0   2.0 Red ginseng   -  -  20.0  - - Red ginseng mixed composition   -  -  -  20.0 - Red ginseng fermented mixed composition   -  -  -  - 20.0 system 1,000 1,000 1,000 1,000 1,000

In Table 2, the AIN mineral mixture (g / kg) contains 620.0 g of calcium lactate, 74.0 g of sodium chloride, 220.0 g of ginseng potassium (basic), 52.0 g of potassium sulfate, 23.0 g of magnesium oxide, 3.3 g of manganese carbonate, 1.0 g of zinc carbonate, 0.2 g of cupric carbonate, 0.01 g of potassium iodate, 0.01 g of sodium selenite, and 0.5 g of potassium chromium sulfate. The final preparation weight is 1,000 g. The AIN vitamin mixture (mg / kg) also contained 600 mg thiamine-hydrogen chloride, 600 mg riboflavin, 700 mg pyridoxine-hydrogen chloride, 3,000 mg nicotinic acid, 1,600 mg calcium D-pantothenate, 200 mg folate, 20 mg D- 2.5 mg, Vitamin A 400.00 IU, Vitamin D3 100,000 IU, Vitamin E 7,500 IU, Vitamin K 75 mg and the final preparation weight is 1,000 g.

2) Experimental animals and breeding methods

Experimental animals were housed in a rats (Sprague-Dawly SPF / VAF outbred rats, Orient, Seongnam, Korea) with an average body weight of 150 ± 10g at 6 weeks of age for 7 weeks in each experimental group.

(Rat & Mouse 18%, PMI Nutrition International, LLC, USA) for 1 week to adapt to the experimental environment. All the diets used in the experiment were prepared once a week to maintain freshness and fed to the experimental animals in a refrigerator at 4 ° C.

Animals were kept in a stainless steel cage at a temperature of 23 ± 2 ° C and humidity of 60 ± 5%. The darkness was automatically adjusted to 12 hours (06: 00 ~ 18: 00).

3) induction of testicular injury

In order to induce testicular damage in animals, Kim et al. (Kim JC, Shin JY, Shin DH, Kim SH, Bae CS, Park SC, Cha SW, Kang BH, Chung MK, Establishment of short-term screening test for detect testicular toxicity in (2) Bromopropane as an example. Laboratory Animal Research 21: 26-34, 2005) and Cha et al. (Cha SE, Park SR, Choi CS, Yoon SH, Acute toxicity of 2-bromopropane in rats, J. Korean Soc Hygienic 2-bromopropane (Sigma Chem. Co., MO, USA) and olive oil were mixed at a ratio of 1: 1 (v / v) 0.2 ml / 100 g BW (body weight) was injected subcutaneously once every 2 days for 3 weeks. The control group was injected with the same amount of olive oil.

4) Testicle size measurement

Testis size was measured three times per week using a micrometer caliper (Mitutoyo, Japan).

5) Weight gain, dietary intake, diet efficiency and drinking water intake

Weight, dietary, and drinking water intake were measured daily at regular intervals. The Food Efficiency Ratio (FER) was calculated by dividing the weight gain during the study period by the total dietary intake.

6) Treatment of experimental animals and preparation of enzyme source

After 4 weeks of feeding, the animals were fasted for 12 hours. Animals fed only with drinking water were anesthetized with ether to collect blood from the abdominal aorta. The testes were sacrificed by the addition of 3 times 0.25 M sucrose solution to the left testis which had been extracted for the purpose of preparing testis tissue. The post-mitochondrial fraction (PMF) obtained by centrifuging the milled homogenate at 10,000 × g for 30 minutes was used for enzyme activity measurement.

7) Determination of the content of glutathione and lipid peroxidation in liver tissue

The content of hepatic tissue glutathione (GSH) was determined by adding 2-nitrobenzoic acid (2-nitrobenzoic acid) to the liver tissue homogenate of the animal according to Ellman's method (Ellman GL: Tissue sulfhydryl group. Arch Biochem Biophys 82: 70-77, 2-nitrobenzoic acid was added to determine the amount of thiophenol produced and the reduced glutathione per gram of liver tissue was expressed in μmol. The content of lipid peroxide (LPO) was determined according to the method of Satho (Satho K., Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method, Clin. Chim. Acta 90: 37-43 1978.) The absorbance of a thiobarbituric acid (TBA) solution was measured by measuring the absorbance of a thiobarbituric acid reactive substance (TBA-reactive substance), which was reacted with n-butanol, at 532 nm, ^{coefficient, ε = 1.5 × 10 5 M} -1 · cm - was expressed as nmol per g liver tissue using a ^first content was calculated.

8) Measurement of glutathione S-transferase and xanthine oxidase activity in testis tissue

The activity of glutathione estransferase was determined by the method of Habig et al. (Habig WH, Pabst MJ, Fleischner G., Gatmaitan F., Aris IM, Jacoby WB, The identity of glutathione S-transferase B with ligandin, 1-chloro-2,4-dinitroezene (CDNB), which is a substrate, and reducing glutathione (CDNB) according to Proc. Natl. Acad. Sci. USA 71: 3879-3882, The thioether produced in this way was measured at a wavelength of 340 nm. The activity was expressed in nmol as the amount of thioether produced by 1 mg protein per minute. The activity of xanthine oxidoreductase was determined according to the method of Stirpe F & Della Corte (Stirpe F., Della Corte E., The regulation of rat liver xanthine oxidase, J. Biol. Chem. 244: 3855-3860, 1969.) The total type activity was measured in the presence of nicotinic acid amide and the content of uric acid produced in the absence of nicotinic acid amide (O type) activity at a wavelength of 292 nm. The activity was measured in the presence of 1 mg / The amount of uric acid produced from xanthine was expressed in nmol. Protein content was determined by the Lowry method (Lowry OH, Rosebrough NJ, Farr AL, Randall RL, Protein measurement folin phenol reagent, J Biol Chem 193: 265-275, 1951) using bovine serum albumin (BSA) ).

Experiment 2. Effect of the Food Composition of the Present Invention on Liver Detoxification, Reactive Oxygen Species (ROS) Cleavage Enzyme Activity and Sexual Function Improvement in Rats Induced by Erectile Dysfunction by Ethanol

1) Preparation of experimental animals and diets

The composition of the experimental diet is shown in Table 3.

Experimental animals were randomly divided into two groups: normal (NC), ethanol control (EN), red ginseng (EN), and ginseng (ER), red ginseng and mixed composition 5% dietary (ERM), and red ginseng and fermented mixed composition 5% dietary (ERFM) Day.

At this time, all the extracts of red ginseng extract, red ginseng plant composition extract and red ginseng fermentation plant mixture composition (each solid content ^o Brix 20) were concentrated to half and the concentration was doubled.

In the control and experimental groups, 50% ethanol solution was orally administered at 0.2g / kg body weight every two days in order to induce impotence caused by alcohol poisoning. For 4 weeks, 50% ethanol solution was orally administered in fasted state once a day and 5 g / kg body weight for 2 days, and then treated 12 hours after the last administration.

The temperature and humidity were adjusted to 23 ± 2 ° C and 60 ± 5%, and the dark and light cycles were set at 12-hour intervals, and water and feed intake were freely consumed.

Dietary composition Experimental group NC EN ER ERM ERFM Solid feed 100 100 95.0 95.0 95.0 Red ginseng - - 5.0 - - Red ginseng mixed composition - - - 5.0 - Red ginseng fermented mixed composition - - - - 5.0

As shown in Table 3, the solid feed is 18 wt% of crude protein, 5 wt% of crude fat, 5 wt% of crude fiber, and 8 wt% of ash in a 5L79 diet of PMI nutrition (LLC, PO Box 19798, Brentwood MO 63144, USA).

2) Weight, Dietary Intake and Drinking Water Intake

Weight, dietary, and drinking water intake were measured at regular intervals throughout the day. Dietary efficiency was calculated by dividing daily gain by daily dietary intake.

3) Sampling of analytical samples

The rats fed the diet for 4 weeks were allowed to drink water freely. After fasting for 12 hours, blood was drawn from the abdominal aorta under ether anesthesia. The liver was perfused with ice-cold physiological saline and the organs were removed. Were measured.

The corpus cavernosum was excised from the lower abdomen of the animal, and the excised penile corpus cavernosum was cleaned with physiological saline, and the remaining foreign matter and blood were removed with a filter paper. Four times the amount of 0.1 M potassium phosphate buffer (pH 7.4) solution per 1 g of corpus cavernosum tissue was added, and homogenized with a homogenizer to obtain a homogenate. The supernatant was centrifuged at 10,000 × g for enzyme activity test .

The collected blood was coagulated at room temperature, centrifuged at 4 ° C and 2,500 rpm for 20 minutes, and the serum was separated and used as an analytical sample at -70 ° C.

4) Measurement of activity of serum alanine aminotransferase

The activity of alanine aminotransferase (ALT) was measured using a kit reagent (ASAN PHARMACEUTICAL, Korea) and the activity was expressed as Karmen unit per 1 mL of serum (Karmen A., A note on the spectrophotometric assay of glutamic oxaloacetic transaminase in human blood serum. J. Clin. Invest. 34: 131-133, 1955.).

5) Glutathione, lipid peroxide and lipid content of liver tissue

The content of hepatic tissue glutathione (GSH) was determined by adding 2-nitrobenzoic acid to the hepatic tissue homogenate of the animal extracted according to Ellman's method (Ellman GL, Tissue sulfhydryl group. Arch Biochem Biophys 82: 70-77, 1959.) (2-nitrobenzoic acid) was added to determine the amount of thiophenol produced, and it was expressed as μmol of reduced glutathione per g of liver tissue. The content of lipid peroxide (LPO) was determined according to the method of Satho (Satho K., Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method, Clin Chim Acta 90 (1): 37-43, 1978.) The absorbance of a thiobarbituric acid (TBA) solution was added to the tissue homogenate and reacted with n-butanol to measure the absorbance of the thiobarbituric acid reactant (TBA-reactive substance) at 532 nm. molecular extinction ^{coefficient, ε = 1.5 × 10 5 M} -1 · cm - was expressed as nmol per g liver tissue using a ^first content was calculated.

The lipid of the liver tissue was analyzed by Folch's method (Folch J., Lees M., Sloane GH, A simple method for isolation and purification of total lipids from animal tissue, J. Biol. Chem. 226: 497-509, 1957) The total lipid content was determined by the method of Frings and Dunn (Frings CS, Dumm RT, A colorimetric method for determination of total serum lipid based on sulfophosphovanillin reaction. Am. J. Clin. Path. 53: 89-91, 1970.).

6) Enzyme activity measurement of corpus cavernosal tissue

A nitrite oxidase synthase, superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT), glutathione S-transferase S-transferase: GST) An enzyme source for active surveillance was prepared by homogenizing an ice-cold 0.25 M sugar solution with a 4-fold amount of the liver tissue of the extracted animal and then centrifuging at 10,000 xg for 20 minutes to obtain a supernatant (postmitochondrial fraction, PMF ) And mitochondrial fractions were used. Nitric oxide synthase activity was measured using the nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase activity assay. The activity of the enzyme was calculated by dividing the amount of protein used in the absorbance value measured at a wavelength of 585 nm.

The superoxide dismutase activity is determined by the method of Martin et al. (Martin, J., Dailey JM, Sugarman, E., Negative and positive assays of hematoxylin autoxidation on the hematoxylin autoxidation assay. Arch. Biochem. Biophys. 255: 329-336, 1987.), and the activity was expressed as the protein content of 50% inhibition of autooxidation of hematoxylin in the absence of enzyme. The activity of glutathione peroxidase is determined by the method described by Pagila & Valentine WN, Studies on the Quantitative and Qualitative Characterization of Erythrocyte Glutathione Peroxidase, J. Lab. Clin. Med. 7: 158-169, The content of nicotinamide adenine dinucleotide phosphate (NADPH), which is used for reducing glutathione reductase to glutathione reductase, was measured at a wavelength of 340 nm. Reduced glutathione, which is consumed for reducing hydrogen peroxide, The amount of nicotinamide adenine dinucleotide phosphate consumed by 1 mg was expressed in nmol. Catalase activity is determined by the method described in Claibome A., Catalase activity in CRC Handbook of Methods for Oxygen Radical Research, ed. RA Greenwald, CRC Press, Florida, pp. 283-284, The activity of glutathione estransferase was determined by the method of Habig et al. (Habig WH, Pabst MJ, Fleischner G, et al. According to Proc Natl Acad Sci USA 71: 3879-3882 (1974), the substrate 1-chloro < RTI ID = 0.0 > Thioether produced by the incorporation of 1-chloro-2,4-dinitroezene (CDNB) and reducing glutathione was measured at a wavelength of 340 nm. The amount of thioether is expressed in nmol I got it.

7) Measurement of protein content

The protein content of liver tissue was determined by the Lowry method (Lowry OH, Rosebrough NJ, Farr AL, Randall RL, Protein measurement folin phenol reagent, J. Biol. Chem., 193 : 265-275, 1951).

Results and Evaluation for Experiment 1

1) Weight gain, dietary intake and diet efficiency

The results of weight, weight gain, dietary intake and diet efficiency of the rats raised for 4 weeks are shown in Table 4.

Body weight was reduced by 22.75% in group B exposed to 2-bromopropane compared to untreated NC group. This decrease was not restored by red ginseng (BR), red ginseng mixed composition (BRM) and red ginseng fermented mixed composition (BRFM) diets, but diet of red ginseng fermented mixed composition caused weight gain in rats.

Dietary intake was slightly decreased in group B than in group N, and the reduction rate tended to be recovered by the red ginseng or red ginseng fermented mixed composition diet, but there was no significant difference. The dietary efficiency of the experimental group was decreased by 20.00% compared to that of group B, and the reduction rate of red ginseng, red ginseng mixed composition and red ginseng fermented mixture composition was not recovered.

The results of this experiment showed that the weight loss of 2-bromopropane-treated animals was significantly lower than that of 2-bromopropane-treated animals (Heo JD, Kim CY, Ziang CZ, Suh JE, Chung MK, Kim MK, Shin DH, The results were consistent with the results of the pollutant 2-bromopropane during pregnancy, Kor J. Lab. Anim. Sci., 20: 89-95, 2004.).

On the other hand, it was suggested that some weight gain in the red ginseng fermented mixed composition diet was related to the increase of the dietary intake and the improvement of the dietary efficiency. In addition, the body weight of animals exposed to chemical substances such as 2-bromopropane in the red ginseng fermented mixed composition diets compared to that of the red ginseng mixed composition diet tended to recover slightly, but the improvement of the physiological activity by fermentation, (Lee YE, Byun SK, Shin SH, Jang JY, Choi BI, Park DS, Jeon JH, Lim SH, Hwang SY, Kim YB, Effect of red ginseng extract on cyclophosphamide teratogenicity in rats. Res. 21: 255-261, 2005).

group Initial weight (g) Final weight (g) Weight gain
(g / week) Dietary intake
(g / week) Dietary efficiency
(FER)   NC   193.50 ± 12.12    399.20 ± 27.60    51.43 + - 5.23    128.80 ± 9.68    0.40 + 0.02   B   201.30 ± 10.16    360.20 ± 39.22    39.73 + - 6.69    125.80 ± 9.52    0.32 ± 0.03   BR   192.40 +/- 7.53    356.30 ± 22.52    40.97 + 4.18    131.30 ± 4.59    0.31 + 0.02   BRM   200.20 ± 14.25    357.60 ± 27.16    39.35 + - 5.59    117.30 ± 11.51    0.34 + 0.02   BRFM   199.60 + - 8.41    369.50 ± 15.76    42.48 ± 2.03    130.70 + - 4.87    0.33 + 0.03

In Table 4, the dietary efficiency was calculated by "total weight gain / total dietary intake" and all data were expressed as "mean ± standard deviation" of 7 animals.

2) Change in testicle size

The testis size of the rats exposed to 2-bromopropane was measured in order to examine the effect of sex enhancement in rats raised for 4 weeks.

The testes of animals were increased by 7.34% in the NC group and 0.12% in group B, and 9.99%, 11.11%, and 11.99% in the red ginseng (BRM) and red ginseng fermented mixed composition (BRFM) . B group, which was treated with 2-bromopropane, did not develop testicular development after growth. These results suggest that 2 - bromopropane induces testicular tissue damage and testicular atrophy. However, after 4 weeks of the experiment, the red ginseng mixed composition and red ginseng fermented mixed composition diet slightly recovered the testis size of the 2 - bromopropane - exposed group compared to the red ginseng alone diet. It is considered that the physiologically active component of the fermented mixed composition other than red ginseng, which is the main component of the complex type, protects the reproductive function as much as possible by minimizing the damage of testicular tissue cells due to 2 - bromopropane toxicity.

group Dietary Period (Week) One 2 3 4   NC       8.31 + - 1.03       8.50 ± 0.56       8.71 ± 0.22       8.92 ± 1.24   B       8.36 ± 0.88       8.25 + - 0.50       8.35 ± 0.45       8.37 ± 0.25   BR       7.61 + - 0.76       8.13 ± 0.37       8.21 ± 0.25       8.37. + -. 0.07   BRM       7.56 ± 0.55       8.20 ± 0.82       8.32 + - 0.37       8.40 + - 0.52   BRFM       7.67 ± 0.37       8.35 ± 0.31       8.45 + 0.22       8.59 + - 0.42

In Table 5, data were expressed as "mean ± standard deviation" of 7 animals.

3) Long weight

The long-term weight of the rats raised for 4 weeks is shown in Table 6 as a percentage of body weight.

Testicular weight of group B decreased by 26.44% compared with that of group N and showed a tendency to increase slightly in the groups of red ginseng (BR), red ginseng mixed composition (BRM) and red ginseng fermented mixed composition (BRFM). The liver weight of group B decreased by 7.14% compared with that of group N, but the liver weight of the BRFM group in the red ginseng fermented mixed composition group was recovered to a level close to that of the untreated group.

Animal experiments with 2-bromopropane as a testicular damage inducer (Kim JC, Shin JY, Shin DH, Kim SH, Bae CS, Park SC, Cha SW, Kang BH, Chung MK. Establishment of short-term screening test test testicular toxicity in rats: 2-Bromopropane as an example. Laboratory Animal Research 21: 26-34, 2005.), testicular and liver weights were decreased compared to normal animals.

Experimental diet slightly restored testis and liver weight reduced by 2 - bromopropane, especially the effect of red ginseng fermented mixed diet on liver function recovery was observed.

These results suggest that the red ginseng fermented mixed composition (BRFM) complex, which has a fermentation process compared to the red ginseng mixed composition (BRM), has specificity for the recovery of liver cells, especially in organ tissues damaged by 2 - bromopropane, Potential efficacy has also been observed for repair of damaged testicular function. In the case of fermented red ginseng mixture, it is presumed that the detoxifying effect and the physiological activity of red ginseng are combined to recover the damaged organs.

group testicle liver kidney spleen Heart   NC      0.87 ± 0.06     3.08 0.30     0.70 + - 0.05     0.17 + 0.04     0.31 + 0.03   B      0.64 0.10     2.96 ± 0.20     0.72 + 0.06     0.21 + 0.02     0.32 ± 0.01   BR      0.68 ± 0.05     2.88 ± 0.18     0.76 + 0.09     0.20 + 0.02     0.33 + 0.03   BRM      0.69 ± 0.08     2.89 ± 0.12     0.78 ± 0.05     0.21 ± 0.06     0.35 + 0.02   BRFM      0.68 ± 0.07     3.01 ± 0.15     0.77 + - 0.13     0.21 ± 0.05     0.34 ± 0.05

In Table 6, all values are percentages by weight and the data are expressed as the "mean value ± standard deviation" of 7 animals.

4) Glutathione and  Peroxide lipid concentration and testicular tissue Xanthine oxidation Glutathione s - TRANSFERASE  activation

After 4 weeks of incubation, glutathione (GSH), lipid peroxide (LPO) and xanthine oxidase (XO) and glutathione S-transferase (GST ) Activity is shown in Table 7.

The glutathione concentration tended to decrease in the group administered with 2-bromopropane, while it was increased in the red fermented mixed composition (BRFM) group. The lipid peroxidation concentration of group B showed a significant increase compared with that of group N, and that of red ginseng fermented mixed composition group was 13.85% lower than that of group B. The activity of xanthine oxidase was increased in 2 - bromopropane - treated group but decreased in red ginseng fermented mixed composition group. Glutathione S - transferase activity increased in the 2 - bromopropane - treated group, especially in the BRM and BRFM groups, by 14.86% and 28.39%, respectively.

In this experiment, the oxidative damage induced by 2-bromopropane resulted in a decrease in liver tissue glutathione concentration in group B, and the recovery of glutathione production was somewhat restored by the fermented red ginseng fermentation (BRFM) diet. Glutathione, an important antioxidant in the body, is thought to be partially recovered from oxidative damage of hepatic tissue by 2-bromopropane through the detoxification (Lin T., Yang MS, et al. GSH level protects against oxidative stress and enhances xenobiotic detoxification in human HepG2 cells. Toxicol. 235: 1-10, 2007.).

Administration of 2-bromopropane in group B provoked a significant increase in lipid peroxidation resulting in oxidative damage to liver tissue. The lipid peroxidation, known as a biomarker of oxidative damage to living tissue, is known to increase with decreasing glutathione concentrations (Casini AF, Pompella A., Comporti M., Glutathione depletion, lipid peroxidation, and liver necrosis following bromobenzene and iodobenzene intoxication. Toxicol Pathol 12: 295-299, 1984). Similar results were obtained in this experiment.

In this experiment, glutathione S - transferase activity of testis tissue is increased in group B compared with that of group N, and it is considered that toxic components such as 2 - bromopropane are detoxified. These results are consistent with the report that injury of the biotissue due to oxidative stress induces the production of antioxidant enzymes including glutathione S-transferase (Yang Y., Yang Y., Trent MB, He N., Lick SD, Zimniak P., Awasthi YC, Boor PJ, Glutathione-S-transferase A4-4modulates oxidative stress in endothelium: possible role in human atherosclerosis. Atherosclerosis 173: 211-21, 2004).

Therefore, it is considered that the group of fermented red ginseng (BRFM) reduces the oxidative damage of the tissue exposed to 2-bromopropane by enhancing glutathione S-transferase activity in liver tissue and testicular tissue.

group Glutathione
(μmole / g)
Lipid peroxide
(μmole / g)
Xanthine oxidation
(uric acid nmole /
mg-protein) Glutathione -
Estransferase
(thioether nmole /
mg-protein / min)   NC      3.26 ± 0.39      43.51 + - 5.76       0.19 + 0.03     134.22 ± 35.26   B      3.17 ± 0.22      59.55 + - 6.78       0.23 + 0.07     152.50 ± 18.02   BR      3.17 ± 0.14      54.23 + - 10.72       0.22 ± 0.06     133.58 ± 18.55   BRM      3.18 ± 0.21      54.25 + - 4.44       0.23 + 0.07     154.16 + - 39.50   BRFM      3.22 ± 0.13      51.30 ± 3.41       0.18 + 0.07     172.33 + - 25.27

In Table 7, data were expressed as "mean ± standard deviation" of 7 animals.

Results and Evaluation for Experiment 2

1) Weight, Dietary Intake and Drinking Water Intake

The results of observing dietary intake, drinking water intake, body weight gain and dietary efficiency of rats induced by erectile dysfunction by chronic ethanol administration during the 4 weeks experimental diet are shown in Table 8.

The daily average weight gain from the differences in body weight and initial body weight was 5.37 g in the normal group, whereas in the ethanol control group it was decreased to 37.0% in the 3.38 g group. On the other hand, in the group of red ginseng (BR), red ginseng mixed composition (BRM) and red ginseng fermented mixed composition (BRFM) group, 22.19%, 26.63% and 30.18% Along with the increase in dietary efficiency, it was observed to restore weight loss due to chronic alcohol metabolism.

group Dietary intake
(g / day) Drinking water intake
(g / day) Weight gain
(g / day) Dietary efficiency   NC   33.74 + 1.40 40.51 + - 3.47 5.37 ± 1.72 1.11 ± 0.15   EN 26.25 + - 3.57 58.23 + - 6.62 3.38 ± 0.65 0.90 + - 0.24   BR 30.38 ± 2.10 51.14 ± 5.26 4.13 ± 0.28 0.95 + 0.17   BRM 31.85 + 3.01 49.55 + - 6.50 4.28 ± 0.58 0.94 + - 0.12   BRFM 31.78 ± 1.61 48.54 ± 3.13 4.40 ± 0.82 0.97 + 0.21

In Table 8, the dietary efficiency was calculated by "daily gain / daily dietary intake" and the data were expressed as "mean ± standard deviation" of 7 animals.

2) Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity

The changes in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity in rats induced by erythropoietin induced by chronic ethanol administration during the 4 weeks experimental diet were shown in FIG. 2 Respectively.

The activity of alanine aminotransferase was significantly lower in all the experimental groups treated with ethanol extract than in the normal group, but the activity of alanine aminotransferase was remarkably lower in ethanol treated group, In the normal group. The activity of serum alanine aminotransferase and aspartate aminotransferase is well known to be increased by hepatic tissue injury and is widely used as an index of hepatic tissue damage in clinical practice (Reitman S. and Frankel S., A colorimetric method for the determination of serum glutamic oxaloacetic pyruvic transaminase. Am. J. Clin. Pathol. 28: 58-63, 1957.).

The significant increase in the activity of serum alanine aminotransferase and aspartate aminotransferase in the ethanol control group was thought to be caused by the toxicity of acute ethanol and the toxicity of acute ethanol was prevented or reduced by the addition of each sample extract Respectively.

3) liver tissue glutathione content, lipid peroxide and neutral lipid content

The results of observing the contents of glutathione, lipid peroxidation and hepatic triglyceride in rats induced by erythropoietin induced by chronic ethanol administration during the 4 weeks experimental diet were shown in Fig.

It is known that glutathione acts to detoxify peroxidized lipids in our body (Ohkawa H., Ohishi N. and Yagi K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction, Anal. Biochem., 95: 248-25 , 1979).

Glutathione contents were increased in rats fed with chronic ethanol (BR), red ginseng mixed composition (BRM), and red ginseng fermented mixed composition (BRFM) diets compared to the experimental control (EN). On the other hand, the lipid peroxidation contents were significantly increased in the EN group, whereas the content of lipid peroxidation was significantly decreased in the group of red ginseng, red ginseng mixed composition and red ginseng fermented mixed composition, and the content was similar to that of the control group (NC).

Lipid peroxidation is known to be an indicator of cell membrane damage as polyunsaturated fatty acids constituting the cell membrane are produced by reactive oxygen species (ROS) (Vladislav E., Dana K., Monika B., The effect of curcumin on cadmium-induced oxidative damage and trace elements levels in the liver of rats and mice. Toxicol Lett 151: 79-85, 2004.). In addition, lipid peroxidase is reduced to the corresponding ethanolic lipid alcohol by glutathione peroxidase and glutathione peroxidase using glutathione, and glutathione is involved in detoxification (Hausburg MA, Dekrey GK, Salman JJ, Palic MR and Gardiner CS, Toxicol. 20: 239-246, 2005.) have been reported to be involved in the development of preimplantation embryos in vivo and in vitro. In addition, it is known that the glutathione content is rapidly increased to prevent or reduce tissue damage after a certain period of time in tissue damage (Wang RS, Nakajima T. and Honma T., Different change patterns of the isozymes of cytochrome p450 and (Lieber CS, Hepatic), and hepatic lipid peroxidation (LH) in the liver, liver, and kidney , metabolic and toxic effects of ethanol. Alcohol Clin. Exp. Res. 15: 573-592, 1991.).

These results suggest that the acute toxicity of ethanol increased the content of hepatic lipid peroxidation in the ethanol control (EN) and the glutathione content was increased due to adaptation of the biomass to alleviate the hepatic tissue damage caused by the ethanol However, in the case of red ginseng (BR), red ginseng mixed composition (BRM) and red ginseng fermented mixed composition (BRFM), various kinds of oil soluble ingredients thereof have an action to prevent or alleviate the acute toxicity of ethanol, And the content of lipid peroxide is low.

Particularly, in the group of fermented red ginseng fermented product (BRFM), the contents of lipid peroxidation and triglyceride were remarkably decreased while glutathione was kept at a normal level. The various useful substances produced by the fermentation and aging of the strain were ethanol- And to prevent and recover from the disease.

The content of triglyceride in hepatic tissues was significantly higher than that of ethanol in chronically orally administered ethanol control group, but the content of hepatic triglyceride was significantly higher than that of normal group. However, red ginseng (BR), red ginseng mixed composition (BRM) Dietary group was significantly decreased. The acute and chronic intake of ethanol is known to increase the triglyceride synthesis of liver tissues, but it is known that the deficiency of apolipoprotein of hypolipidemic lipoprotein (VLDL) causes accumulation of neutral lipids in liver tissues and accumulation of fatty liver (Lieber CS, Hepatic, metabolic and toxic effects of ethanol. Alcohol Clin. Exp. Res. 15: 573-592, 1991.). Therefore, it is considered that the increase of hepatic triglyceride content in the ethanol control group is caused by the acute toxicity of ethanol. Based on the above results, in case of the fermented mixed composition containing red ginseng, the toxicity by ethanol is prevented or reduced It may be helpful to improve the erectile dysfunction symptoms by facilitating the metabolism that affects erectile dysfunction.

4) Activity of enzymes in the erythrocyte scavenging system of corpus cavernosum

Erectile dysfunction caused by organic causes is divided into endocrine, neurogenic, vascular, systemic and other causes. In particular, ethanol reduces erectile function and causes irreversible prostate atrophy and loss of spermatocytes and sperm cells in alcoholic men, even in the absence of liver damage.

Erectile dysfunction is a phenomenon in which blood can not flow normally into the corpus cavernosum tissue, and normal inflow of blood and congestion can improve erectile dysfunction. It is known that the vascular relaxation response in the corpus cavernosum is caused by vascular endothelial relaxation factor, nitric oxide (NO), which is a type of hormone present in vascular endothelial cells. Nitric oxide is biosynthesized by the biochemical action of nitric oxide synthase (NOS) in the body.

To evaluate the effect of active oxygen scavenging enzymes on the activity and stress of nitric oxide synthase, we measured the effects of erectile dysfunction induced by chronic ethanol administration during 4 weeks of experimental diet. The changes in nitric oxide synthase, superoxide dismutase, glutathione peroxidase, catalase and glutathione-esterspreferase activity of the corpus cavernosum tissue are shown in Table 9.

The activity of nitrite oxide synthase, superoxide dismutase, glutathione peroxidase, catalase, glutathione estransferase activity in BR, red ginseng mixture (BRM) and red ginseng fermented mixed composition (BRFM) Was significantly increased compared to the ethanol control group, especially in the red ginseng fermented mixed composition diet group.

Superoxide dismutase converts excess oxygen (O ₂ ^- ) to toxic one-step hydrogen peroxide (H ₂ O ₂ ), and catalase and glutathione peroxidase ultimately detoxify hydrogen peroxide into water. In addition, glutathione estransferase can be used as an active oxygen species when it is not removed, and when it is made into lipid peroxides, it is converted to an ethanolic fat with low toxicity using glutathione (Im MJ, Manson PN, Bulkley GB, Hoopes JE , Effects of superoxide dismutase and allopurinol on survival of acute island skin flaps. Ann. Surgery 201: 357-359, 1985 .; Jacoby JB, The glutathione S-transferase: a group of multifunctional detoxification proteins. Mol Biol 46: 383-414, 1978.).

As a result, the extract of fermented red ginseng composition was found to be effective in increasing the activity of antioxidant enzymes of the testes. In addition, acute and chronic ethanol administration also promoted the activity of nitric oxide synthase to prevent tissue damage caused by ethanol, It was observed that the symptoms could be alleviated.

group Nitric oxide-synthase Superoxide-dismutase Glutathione peroxidase Catalase Glutathione - Estransferase   NC   1.85 ± 0.10  31.25 + - 2.20  88.15 + - 5.59 282.15 + 15.22 432.75 ± 25.43   EN   1.05 + - 0.05  12.57 + - 5.78  55.41 ± 5.12 124.54 ± 19.32 371.27 ± 33.85   BR   1.48 0.12  42.53 + - 2.55  75.16 + - 6.23 209.57 ± 15.47 398.02 + - 15.05   BRM   1.52 + 0.15  51.63 + - 4.78  82.15 ± 5.18 221.28 ± 10.92 453.53 + - 22.50   BRFM   1.65 + 0.11  55.27 ± 5.22  81.88 + - 6.78 230.43 + - 18.55 472.12 + - 18.98

In Table 9, the unit of nitric oxide-synthase unit is? Absorption / protein mg, the unit of superoxide dismutase is unit (the content of protein that inhibits 50% of autoxidation of hematoxylin), and glutathione peroxidase Unit of nmole / protein mg, the unit of catalase: nmole / protein mg, and the unit of glutathione estransferase is nmole / protein mg. The data were also expressed as "mean value ± standard deviation" of 7 animals.

From the above experimental results, the following facts were confirmed.

First, the effects of 2-bromopropane (testis damage inducer) on the testis and other organs of rats and the effects of oxidative damage on the liver and testicular tissues were compared. Compared with red ginseng alone, the composition and composition of the fermented red ginseng composition slightly restored the testis size of the 2-bromopropane-exposed group, and were specific for the recovery of liver cells, especially of organ tissues damaged by 2-bromopropane, Potential efficacy has also been observed for repair of damaged testicular function. In addition, the red ginseng fermented mixed diet group increased the activity of antioxidant enzymes in liver and testicular tissues, and it alleviated the oxidative damage of tissues.

Next, the effects of the red ginseng fermentation mixture composition on liver detoxification, reactive oxygen species (ROS) cleavage enzyme activity and sexual function improvement in rats induced by erectile dysfunction caused by ethanol were examined from liver tissue and penis As a result of comparing the effects on the corpus cavernosum, the content of glutathione involved in detoxification was increased, the peroxide content was restored to the normal group level, and the effect of significantly lowering the triglyceride content was confirmed. Serum alanine aminotransferase activity, which indicates the degree of liver damage, recovered to normal levels in all experimental groups. The activity of antioxidant enzymes that inhibit the action of reactive oxygen species was high in the extract of mixed composition of fermented red ginseng. The extract of fermented red ginseng composition was found to be effective in increasing the activity of antioxidant enzymes of the testes and the activity of acute and chronic ethanol It was also observed that administration of nitric oxide synthase improves the activity of ethanol to prevent tissue damage and alleviate erectile dysfunction.

It is to be understood that the invention is not limited to the disclosed embodiment, but is capable of many modifications and variations within the scope of the appended claims. It is self-evident.

Claims (5)

delete delete A pretreatment step of pretreating a raw material of a fermented mixed powder composed of maca, damiana, pumpkin seed, soybean, ginger, cordyceps, corn oil, gougia, silkworm, and bamboo;
Mixing the pretreated raw material with a fermenting microorganism and aging the mixture to prepare a fermentation mixed powder;
Mixing the fermentation mixed powder and the red ginseng powder to prepare a red ginseng fermentation mixed powder;
Extracting and concentrating a part of the red ginseng fermentation mixed powder to prepare a concentrated fermentation extract concentrate;
Mixing the red ginseng fermentation mixed powder with the mixed fermentation extraction concentrate and drying the mixture to prepare a red ginseng mixed composition;
Wherein the fermented red ginseng composition comprises a fermented red ginseng mixture.
The method of claim 3,
Wherein the pretreatment step comprises autoclaving, roasting, rapid freeze-drying, and pulverizing the raw materials, respectively.
The method of claim 3,
The fermenting mixed composition of red ginseng is prepared by spraying 10 parts by weight of the concentrated fermentation broth with the red ginseng fermentation mixed powder and drying the mixture so that the moisture content is 5% or less. ≪ / RTI >

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