KR20190113721A - Onion Coat composition Using An Enzyme With Stevia Extract And Method thereof - Google Patents

Abstract

The present invention relates to an onion peel enzyme composition comprising discarded onion peel and stevia comprising an extract of a stevia plant which is and a natural sugar plant as an active ingredient, and a method for manufacturing the same. The feed containing the composition is fed to animals to increase disease resistance against foot-and-mouth disease and bird flu.

Description

Onion Peel Enzyme Composition Containing Stevia and Its Preparation Method {Onion Coat composition Using An Enzyme With Stevia Extract And Method}

The present invention relates to an onion peel enzyme composition comprising stevia and a method for preparing the same, and more particularly, to onion peel enzyme feed comprising stevia including stevia, which is discarded onion peel and natural sugar plant, as an active ingredient and its preparation It is about a method.

Onium (Allium cepa) is a biennial herb of the monocotyledonous lily. Onion's food composition is somewhat different depending on various environmental factors such as varieties, harvest season, soil, climate, etc., but it usually contains 90% water, 8% sugar and 1.0% protein.

Onion is a vegetable with a lot of protein, and contains glucose, sugar, fructose, maltose, etc. It has a unique sweetness and is rich in minerals such as potassium, calcium, iron, phosphorus and sodium, dietary fiber and folic acid. It contains 20mg, low sodium content and no fat.

Onions contain a large amount of quercetin, a yellow flavonoid, which is also a bitter ingredient in green tea, spinach, broccoli, parsley, and onions. In addition to preventing the oxidation of unsaturated fatty acids that make up the body to remove the free radicals generated in the body has a great effect in preventing cancer and aging, hypertension, hyperlipidemia, diabetes, diet, detoxification, cold, osteoporosis, constipation, insomnia, It is effective in increasing memory, dementia, neuralgia, depression, hair loss, skin care, eye diseases, anti-inflammatory, anti-allergy, food poisoning, gastritis, kidneys, prostate, eczema, athlete's foot, heavy metals, yellow dust.

These onions have long been used in many foods as healthy vegetables. Onions are mainly edible, and the peculiar smell of scales is due to compounds such as propyl disulfide and allyl sulfide. This physiologically promotes the secretion of digestive juices and has the effect of excitement, sweating and diuresis.

In addition, the scales contain minerals such as calcium and phosphoric acid together with various vitamins to remove harmful substances in the blood. Scales are often used in salads, soups, and meat dishes, and are used as spices in various dishes. The leaves contain vitamin I 5,000 IU, vitamin C 45 mg, calcium 80 mg, magnesium 24 mg and potassium 220 mg in 100 g.

In addition, the onion peel contains a quercetin-flavonoid compound containing about 10 to 100 times higher than the edible portion of the onion. In particular, more than 2.5% of the dry skin of the onion is quercetin, and since it exists in the free state rather than in the glycoside form, the biological activity is stronger than the onion's edible part. However, in general, the onion peel is removed when processing the onion, and only the decorative part of the onion is used, and the removed onion peel contains various bioactive substances including quercetin, but most of them cannot be applied to products for other purposes. It is losing.

That is, although the excellent components of these onions are generally contained in the onion skin most, there is a problem that most of them are incinerated by treating waste in the onion pretreatment process.

Therefore, there is a need for technical improvements to the technology for providing feed having disease resistance by reusing the discarded onion peel to solve the above problems.

Registered Patent Publication No. 10-0775630 (Registration Date: 2007.11.05)

The present invention aims to solve the problems of the prior art as described above and the technical problems that have been requested from the past.

An object of the present invention, by using the discarded onion shells to feed the animals containing the natural sugar plant stevia as an active ingredient to onions containing stevia that can improve disease resistance to foot and mouth disease, bird flu and collet It is to provide a method for producing a shell enzyme feed and the enzyme feed produced thereby.

In addition, another object of the present invention, by providing an enzyme extract containing a stevia extract which is a natural sugar plant, it has excellent sweetness effect to reduce the spicy taste of the original onion to include stevia easy to drink for children or the general public To provide an onion peel enzyme feed production method and the enzyme feed produced thereby.

Onion peel enzyme composition comprising stevia in the present invention for achieving this object, may include an extract derived from a mixture of onion peel 70% to 95% by weight and stevia 30% by weight to 5% as an active ingredient. Can be.

In one preferred embodiment of the present invention, the stevia may be steviol, steviol precursor, or extract containing at least one or more of stevioside and / or rebaudioside A. .

In one preferred embodiment of the present invention, the onion peel enzyme composition may be provided in the form of a pill, powder or capsule, but is not limited thereto.

In one preferred embodiment of the present invention, the onion peel extract may be a crude extract prepared by a solvent extraction or hot water extraction method.

In one preferred embodiment of the present invention, the solvent may be purified water, alcohol having 1 to 5 carbon atoms or organic solvent.

Here, when the solvent is an alcohol, it may be ethanol, methanol, propanol, butanol, ethanol aqueous solution or methanol aqueous solution.

On the other hand, the present invention provides an enzyme feed comprising the onion peel enzyme composition.

The present invention also provides a method for preparing an onion peel enzyme composition comprising stevia comprising an extract derived from a mixture of 60% to 95% by weight of onion peel and 40% to 5% stevia plant as an active ingredient. to provide.

In one preferred embodiment of the present invention, the extract can be prepared by enzymatically undergoing a aging process for 2 to 4 months.

As described above, the onion peel enzyme composition comprising stevia according to the present invention, by using the discarded onion peel to feed animals containing stevia, a natural sugar plant as an active ingredient, foot and mouth disease, bird flu and collet There is an effect that can improve the disease resistance to the back.

In addition, by providing an enzyme extract comprising a stevia extract which is a natural sugar plant, it has excellent sweetness effect, thereby reducing the spicy taste of the original onion is easy to drink for children or the general public.

1 is a flowchart illustrating a process of preparing an onion peel composition comprising stevia according to an embodiment of the present invention.

In the following description of the invention, reference is made to specific embodiments in which the invention may be practiced. These embodiments are described in sufficient detail to enable those skilled in the art to practice the invention. It should be understood that the various embodiments of the present invention are different but need not be mutually exclusive. For example, specific shapes, structures, and characteristics described herein may be implemented in other embodiments without departing from the spirit and scope of the invention with respect to one embodiment.

The following detailed description, therefore, is not to be taken in a limiting sense, and the scope of the present invention, if properly described, is defined only by the appended claims, along with the full range of equivalents to which such claims are entitled.

In addition, in the present invention, although the first, second, etc. are used to describe various components, these components are of course not limited by these terms. These terms are only used to distinguish one component from another.

Hereinafter, preferred embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention.

As described above, conventionally, onion peel containing the most excellent ingredients is disposed of without reuse, causing environmental pollution.

Therefore, in the present invention, an onion peel enzyme composition comprising stevia comprising an extract derived from a mixture of 60% to 95% by weight of onion peel and 40% to 5% of stevia plant as an active ingredient is prepared for animal feed. The solution to the above-mentioned problems was sought. Through this process by processing the onion peel to be environmental pollution containing a certain amount, it is possible to provide an onion peel enzyme feed containing stevia and a manufacturing method thereof.

According to the present invention, the onion peel enzyme composition may be prepared in a pill and provided as a feed for livestock, or may be provided in the form of powder or capsule, which can be directly consumed by a person.

"Feed" in the present invention is any natural or artificial diet, single meal, or the like, or as a component of the single meal for the animal to eat, ingest, digest, or according to the present invention, a mixture of onion peel and stevia or stevioside Feed containing a feed additive comprising as an active ingredient can be prepared in various forms of feed known in the art, preferably rich feed, forage and / or special feed may be included

As used herein, the term “food product” refers to any food or feed suitable for consumption by humans or animals.

The term “food supplement” refers to a small amount of compound for supplementing a human or animal meal packaged in single or multiple dosage units. Food supplements generally do not provide significant amounts of calories but may contain other micronutrients (eg, vitamins or minerals).

The term "nutrition supplement" means a composition comprising a food supplement with a calorie source. In some embodiments, the nutritional supplement is a meal replacement or supplement (eg, a nutrition or energy bar or a nutritional drink or concentrate).

As used herein, “prevention” is not intended to mean that a given event will never occur, but rather delays the onset of a given state or event and reduces the severity of the condition or event when the condition or event occurs.

First, the onion peel of the present invention is used by pulverizing itself or extracted by an organic solvent or an extraction method using water, preferably purified or treated with ethyl alcohol, water or a mixture or water thereof. Use it. In addition, as the organic solvent, it is preferable to use a fermentation alcohol which has little harm to the human body, but any organic solvent may be used as long as it is a pharmaceutically acceptable organic solvent.

However, the present invention can be used to include an effective amount in the composition even if the onion peel extracted or produced by any method other than the above method.

According to the present invention, the solvent may be purified water, an alcohol having 1 to 5 carbon atoms or an organic solvent, and when the solvent is an alcohol, the solvent may be ethanol, methanol, propanol, butanol, ethanol aqueous solution or methanol aqueous solution.

According to the invention, with respect to 100 parts by weight of the total onion peel composition, the onion peel extract may comprise from 60% by weight to 95% by weight. Preferably it may comprise 80 to 95% by weight, more preferably may comprise 85% by weight. At this time, when the onion peel extract is less than 60% by weight may have a problem of reducing the pathogenic resistance during the manufacture of the feed, when the onion peel extract exceeds 95% by weight the desired sweetness of the feed falls and onions Ingestion by inherent spicy ingredients may not be easy, so the above range is good.

In some cases, when prepared in the form of a pharmaceutical preparation, a composition comprising onion peel extract is combined with a pharmaceutically acceptable conventional carrier, and according to the purpose of administration, tablets, hard or soft capsules, chewing tablets , Oral formulations such as powders, solutions or suspensions, or parenteral administration such as injectable solutions or suspensions.

At this time, when the onion peel extract of the present invention is formulated into a formulation for oral administration such as tablets, capsules, chewing tablets, powders, solutions, suspensions, and the like, gum arabic, corn starch, microcrystalline cellulose or gelatin Binders, excipients such as dicalcium phosphate or lactose, disintegrants such as alginic acid, corn starch or potato starch, lubricants such as magnesium stearate, sweeteners such as sucrose or saccharin and peppermint, methyl salicylate or fruit flavor Flavoring agent may be included, and when the dosage unit type is a capsule, a liquid carrier such as polyethylene glycol or fatty oil may be included in addition to the above components.

On the other hand, the stevia is a perennial plant belonging to the Asteraceae family and is a natural sweetening source from Paraguay, and steviaside sweetness has several hundred times sweeter sugar than sugar and has very few calories, so it is the main ingredient of general food and health functional food Can be written as

According to the present invention, the stevia may comprise an extract containing at least one of steviol, steviol precursor, or stevioside and / or rebaudioside A.

Here, the steviol precursor means steviol glycoside which can be hydrolyzed by an enzyme to become a free non-saccharide steviol. See, eg, EFSA Journal 2010, 8 (4): 1537, which is incorporated herein by reference. Suitable enzymes for this hydrolysis are present in certain microorganisms and also in the intestines of humans or animals. For example, Gardenana et al., 2003, J. Agric. Food Chem. 51: 6618-22.

According to the invention, with respect to 100 parts by weight of the total onion skin composition, the stevia extract may comprise 40% by weight to 5% by weight. Preferably it may be included in 30 to 5% by weight, more preferably may be included in 15% by weight. At this time, when the onion peel extract is less than 5% by weight it may be difficult to use the feed because it is difficult to produce the desired sweet taste, when the stevia extract exceeds 40% by weight, reducing the pathogenic resistance and efficacy of the feed It is possible to make the above range is good.

In addition, according to the present invention, it provides an enzyme feed comprising the onion peel enzyme composition and fertilizer in a content ratio of 3: 7 to 2: 8. Here, when the onion peel enzyme composition and fertilizer is out of the range of 2 to 3 content ratio, it is not possible to produce a feed containing a desired antimicrobial effect, so the above range is good.

Hereinafter, a method for preparing an onion peel enzyme composition comprising stevia according to the present invention will be described.

The present invention provides a method for producing an onion peel enzyme composition comprising stevia comprising an extract derived from a mixture of 60% to 95% by weight of onion peel and 40% to 5% of stevia plant as an active ingredient. do.

Figure 1 is a flow diagram of a method for producing an onion peel enzyme composition comprising stevia according to one embodiment of the present invention.

Specifically, as shown in Figure 1, the onion peel enzyme composition comprising stevia according to the present invention, the process of collecting the stevia plant and washing with drinking water (S1), the process of cutting and grinding the washed stevia plant ( S2), the process of collecting and washing the onion peel with drinking water (S3), the process of preparing a mixture by mixing the stevia plant and the ground onion peel (S4), the stevia plant and the crushed onion peel mixture It is put into the extractor and heated to 80 to 150 degrees, and the process of cooling the mixture is heated slowly at room temperature (S5) and the process of aging the mixture for 2 to 4 months at 55 to 75 degrees (S6).

First, the stevia extract and onion peel extract is prepared through the process of washing and grinding using the stevia (S1 ~ S3).

According to the present invention, the onion peel may be used by grinding, and in some cases, may be prepared as an extract through a separate extraction process.

When explaining the onion peel extraction process, the onion peel extract can be prepared using the hot water extraction method.

Specifically, the onion is washed using the water pressure of drinking water to remove foreign substances such as soil and microorganisms remaining, and the onion peel separated by the water pressure during the washing process is removed to remove portions other than onion peel such as onion roots. Secure onion skin. The selected onion peel is dried in the cow, and the dried onion peel is ground using a dry grinder of the pin crusher or catamyl method to prepare an onion peel powder. Depending on the design conditions, the onion peel may be pulverized using various types of dry pulverizers such as multi-purpose grinders, hammer mills and the like.

The crushed onion peel powder is made into a concentration of 10 ~ 20 ㎎ / ㎖ using purified water and then hot water extraction (heat extraction) at 80 ~ 100 ℃, pH 6 ~ 7 for 30 to 60 minutes.

Preferably, the onion peel powder concentration is 20 mg / ㎖, temperature is 100 ℃, pH is adjusted to pH 6 using a phosphate buffer solution to extract hot water for 30 minutes. This is a hot water extraction condition to obtain an extract containing a high content of bioactive substances from onion skin powder.

In addition, according to the present invention, the stevia plant may be used by mixing with the onion skin pulverized or extracted, or may be prepared through a separate extraction process.

In describing the stevia extraction process, stevia leaves and stems can be used as the stevia plant tissue, and depending on the intended use, the extract can be extracted using only the stevia leaves, or the extract can be extracted using only the stevia stem. The extract can also be extracted by mixing a proper amount with the stem.

Extraction treatment of plant tissues such as stevia leaves and stems is dried and pulverized plant tissues, put the pulverized crushed product in an extraction container containing water and heated to extract the extract from the plant tissues of stevia. In order to extract the extract liquid from the pulverized product more effectively, another solvent may be mixed with water, and the extract may be extracted from the pulverized product at a pressure higher than atmospheric pressure.

When extracting the extract liquid from the pulverized product at a pressure higher than atmospheric pressure, the boiling point of the water containing the pulverized product is increased, so that the active ingredient contained in the stevia plant tissue can be efficiently extracted.

Specifically, first, stevia leaves with a rebaudioside content of 10% by weight or more are harvested on the stevia leaves, washed with water to remove foreign substances such as dust, and left in a well-ventilated shade for about 3 hours so that the leaves turn green. To dry. If the rebaudioside content of stevia leaves with a stevia leaf content of 10% by weight or more is selected and used, less bitterness, rebaudioside can reduce the strong sweetness unique to stevioside can improve the palatability.

Here, the process of cutting and drying the stevia leaves is repeatedly dried until the moisture content is 45 to 55% by weight. In some cases, it is preferable to rub the dried stevia leaves with a bibimator to destroy the cell walls of the stevia leaves.

At this time, the moisture content of the stevia leaf is important, since it uses only an onion peel, there is almost no moisture, so the moisture of the stevia leaf can be appropriately used. When the moisture content of the stevia leaf is less than 45% by weight, fermentation may not be smooth, and when the moisture content exceeds 55% by weight, harmful fungi may breed during fermentation.

Next, the process of mixing the stevia extract and onion peel extract prepared as described above (S4). As described above, the mixture prepared from the stevia extract and the onion peel extract is put into an extractor, and the mixture is added to a high speed mixer and mixed before heating.

Then, the process of heating and drying the mixture prepared from the onion peel extract and the stevia extract is performed (S5). It may be performed repeatedly for 5 to 30 minutes at approximately 8-150 degrees.

By heating the mixture, stevioside and moisture in the stevia plant are extracted in addition to the extract and water in the onion peel by the pressure and high temperature of the hot steam to prepare an extract containing sweetness.

Next, if necessary, go through a fermentation process, the concentrate is fermented at a temperature of 20 to 30 degrees. At this time, it is preferable to inject oxygen into the concentrate at regular intervals. Periodically injecting oxygen into the concentrate as yeast bacteria involved in fermentation are mainly aerobic microorganisms, which promotes fermentation and proliferation of yeast bacteria involved in fermentation. Providing there is an advantage that can prevent the fermentation caused by the decay bacteria during fermentation.

Finally, a aging process is performed, and this aging process may be performed at about 55 to 75 degrees for about 2 to 4 months to prepare an onion peel enzyme composition including stevia extract.

Here, according to the present invention, preferably the aging process (S7) may be aged for 2.5 to 3.5 months at 60 degrees to 70 degrees, more preferably at 65 degrees for three months.

Hereinafter, preferred examples will be presented to aid in understanding the present invention. However, the following examples are only for the understanding of the present invention, and the present invention is not limited to the following experimental examples.

[Production example]

Preparation Example 1 Onion Peel Extract

Cut onion skin (Fomitopsis pinicola (Sw.ex Fr.) Karst.) Into 23cm pieces, add 2l of 60% (v / v) ethyl alcohol per 100g dry weight, extract at 60 ℃, and then sieve (0.054mm) and filter paper ( The extract was obtained by filtration using Whatman No 2).

Preparation Example 2 Stevia Plant Extract

Stevia leaves and stems were dried, pulverized and mixed, and then placed in permeable heat-resistant sacks 3kg each. Into the extraction vessel containing water, two heat-resistant sacks containing crushed powder were added, and heated to a temperature of 110 ± 10 ℃ for 2.5 hours to extract the active ingredient contained in stevia. The extracted extract was placed in a concentration bath and heated to a temperature of 110 ± 10 ° C. under negative pressure for 13 to 15 hours while stirring to obtain a concentrate. The concentrated solution was put in a fermentation vessel, and then fermented at 20 to 30 ° C. for 5 days under granular activated carbon treatment, and then aged at about 20 to 30 ° C. for about 150 days to complete stevia extract having a suitable antioxidant index. It was.

EXAMPLES Preparation of Onion Peel Composition and Enzyme Feed Comprising Stevia

<Example 1>

85% by weight of the onion peel extract and 15% by weight of stevia extract prepared in Preparation Example 1 and Preparation Example 2 were added to the aquaculture feed, then added to the extractor and heated at a temperature of 120 ° C. for 2.5 hours and stirred to concentrate the concentrate. Got it. Then, the concentrate was placed in a fermentation vessel and then treated with granular activated carbon, followed by a fermentation process at 25 ° C. for 5 days, and then aged at 90 ° C. for about 90 days to prepare feed in a pill.

<Example 2>

A feed in the form of a cyclic form was prepared in the same manner as in Example 1 except for blending 60 wt% of the onion peel extract and 40 wt% of the stevia extract.

[Comparative Example]

Comparative Example 1

Except for the use of fructose as sugar instead of stevia extract was prepared in the form of a feed in the same manner as in Example 1.

Comparative Example 2

A feed in the form of a ring was prepared in the same manner as in Example 1 except that granular activated carbon was not treated.

Experimental Example

Experimental Example 1

The concentrates of Examples 1 to 2 and Comparative Examples 1 to 2 were compared with the fermentation period, the degree of corruption, the sensory test of the concentrate during fermentation, and the aging time for obtaining an extract having a suitable antioxidant index. Shown in In this case, the degree of corruption in the fermentation process was indicated by '○' if the occurrence of corruption was visually confirmed, '△' if only a part was confirmed, and '×' if not confirmed. Tests were evaluated by five-point scoring method (9-very good, 7-good, 5-normal, 3-bad, 1-very bad) for five males and five females.

Fermentation period Corruption Odor during fermentation Ripening period Example 1 5 × 7.50 90 Example 2 5 × 6.40 90 Comparative Example 1 5 × 3.40 120 Comparative Example 2 9 O 2.70 150

As can be seen in Table 1, when the granular activated carbon is treated in the concentrate during the fermentation process as in Examples 1 and 2, the odor due to decay and the peculiar odor generated during fermentation are not treated in Comparative Example 2 It was found that relatively less than in comparison, and also significantly reduced fermentation period and maturation period. In addition, Comparative Example 1, which was treated with activated charcoal but used fructose instead of stevia, had a slightly increased odor during fermentation and a aging period was slightly increased compared to Example 1.

Experimental Example 2

Sensory evaluation was performed on the sweetness, color, aroma, taste and premise preference for the enzyme feeds of Examples 1 to 2 and Comparative Examples 1 to 2. At this time, the nine-point scoring method (9-very good, 7-good, 5-medium, 3-bad, 1-) for 20 adult adults and females was concentrated on the concentrate prepared by mixing onion peel extract and stevia extract. Very bad) and the sensory evaluation, and the results are shown in Table 2.

sweetness color incense smell Symbol Example 1 8.3 8.2 8.3 8.1 8.2 Example 2 9.5 8.9 8.6 8.5 8.6 Comparative Example 1 2.1 7.5 7.3 7.1 6.7 Comparative Example 2 8.3 8.9 6.1 5.4 7.2

As can be seen in Table 1, Examples 1 to 2 and Comparative Example 2 containing a stevia in a predetermined content ratio and Comparative Example 1 containing a fructose instead of stevia, such as sweetness, color, odor, etc. It's better than ever. However, in the case of Comparative Example 2 which was not treated with activated carbon, it was found that the overall acceptability was slightly decreased due to the increase in flavor and smell.

Although described with reference to the drawings according to an embodiment of the present invention, those of ordinary skill in the art will be able to perform various applications and modifications within the scope of the present invention based on the above contents.

Claims (10)

An onion peel enzyme composition comprising stevia comprising an extract derived from a mixture of onion peel 70% to 95% by weight and stevia 30% by weight to 5% as an active ingredient.
The method of claim 1,
The stevia is an onion peel enzyme comprising stevia, which is an extract containing at least one of steviol, steviol precursor, or stevioside and / or rebaudioside A. Composition.
The method of claim 1,
The onion peel enzyme composition is an onion peel enzyme composition comprising stevia, characterized in that provided in the form of a ring, powder or capsule.
The method of claim 1,
The onion peel extract is an onion peel enzyme composition comprising stevia, characterized in that the extract is prepared by a solvent extraction or hot water extraction method.
The method of claim 4, wherein
The solvent is onion peel enzyme composition comprising stevia, characterized in that the purified water, alcohol having 1 to 5 carbon atoms or an organic solvent.
The method of claim 4, wherein
The solvent is onion shell enzyme composition comprising stevia, characterized in that ethanol, methanol, propanol, butanol, aqueous ethanol or aqueous methanol solution.
Enzyme feed comprising an onion peel enzyme composition and feed according to any one of claims 1 to 6 in a content ratio of 3: 7 to 2: 8.
The method of claim 7, wherein
The enzyme feed is an enzyme feed, characterized in that the onion shell enzyme composition and feed comprising a weight ratio of 3: 7 to 2: 8.
Method for preparing an onion-shell enzyme composition comprising stevia comprising an extract derived from a mixture of onion peel 70% to 95% by weight and stevia 30% by weight to 5% as an active ingredient.
The method of claim 9,
The extract is a method for producing an onion peel enzyme composition comprising stevia, characterized in that it is enzymatically subjected to aging for 2 to 4 months.

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