KR20210141915A - 표적화된 핵산 라이브러리 형성을 위한 방법 - Google Patents

표적화된 핵산 라이브러리 형성을 위한 방법 Download PDF

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KR20210141915A
KR20210141915A KR1020217018861A KR20217018861A KR20210141915A KR 20210141915 A KR20210141915 A KR 20210141915A KR 1020217018861 A KR1020217018861 A KR 1020217018861A KR 20217018861 A KR20217018861 A KR 20217018861A KR 20210141915 A KR20210141915 A KR 20210141915A
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adapter
probe
extension product
nucleic acid
primer
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헹 왕
솅롱 린
그레이스 자오
연 바오
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아비다 바이오메드 인코포레이티드
헹 왕
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020217018861A 2018-11-21 2019-11-20 표적화된 핵산 라이브러리 형성을 위한 방법 Ceased KR20210141915A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862770585P 2018-11-21 2018-11-21
US62/770,585 2018-11-21
PCT/US2019/062507 WO2020106906A1 (en) 2018-11-21 2019-11-20 Methods for targeted nucleic acid library formation

Publications (1)

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KR20210141915A true KR20210141915A (ko) 2021-11-23

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KR1020217018861A Ceased KR20210141915A (ko) 2018-11-21 2019-11-20 표적화된 핵산 라이브러리 형성을 위한 방법

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Country Link
US (1) US20210355485A1 (https=)
EP (2) EP4644568A3 (https=)
JP (1) JP2022513124A (https=)
KR (1) KR20210141915A (https=)
CN (1) CN114901818B (https=)
AU (1) AU2019384154B2 (https=)
CA (1) CA3120713A1 (https=)
GB (2) GB2615955B (https=)
WO (1) WO2020106906A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
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WO2024005523A1 (ko) 2022-06-28 2024-01-04 주식회사 제노헬릭스 Rna 염기서열 분석을 위한 라이브러리 제작 방법
KR20240002713A (ko) 2022-06-28 2024-01-05 주식회사 제노헬릭스 Rna 염기서열 분석을 위한 라이브러리 제작 방법
KR20250033954A (ko) 2023-08-30 2025-03-10 주식회사 제노헬릭스 제한효소를 이용한 rna 라이브러리 제작 방법

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CN111254500B (zh) 2012-12-10 2024-01-23 分析生物科学有限公司 靶向基因组分析的方法
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
JP6913089B2 (ja) 2015-11-11 2021-08-04 レゾリューション バイオサイエンス， インコーポレイテッド Ｄｎａライブラリーの高効率構築
US11319594B2 (en) 2016-08-25 2022-05-03 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
WO2022055984A1 (en) * 2020-09-08 2022-03-17 Resolution Bioscience, Inc. Adaptors and methods for high efficiency construction of genetic libraries and genetic analysis
ES2960777T3 (es) * 2021-03-18 2024-03-06 Genomill Health Oy Métodos para la cuantificación paralela precisa de ácidos nucleicos en muestras diluidas o no purificadas
EP4215619A1 (en) * 2022-01-21 2023-07-26 Genomill Health Oy Methods for sensitive and accurate parallel quantification of nucleic acids
US20250101412A1 (en) * 2022-01-25 2025-03-27 Oregon Health & Science University Methods and systems for increasing capture potential of a spatial array
KR20240150780A (ko) * 2022-02-18 2024-10-16 애질런트 테크놀로지스, 인크. 표적 핵산 포획 및 바코딩을 위한 시스템 및 방법
KR20250017217A (ko) * 2022-05-27 2025-02-04 쳉-야오 첸 핵산의 양쪽 표지를 위한 방법, 키트 및 시스템
WO2025221668A1 (en) * 2024-04-19 2025-10-23 Agilent Technologies, Inc. Methods of preparing nucleic acids for sequencing and methylation analysis
US12509733B1 (en) 2025-04-07 2025-12-30 Inivata Ltd. Methods and systems for detecting a primary disease

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FR2724934B1 (fr) * 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique
WO2001057268A2 (en) * 2000-02-07 2001-08-09 Illumina, Inc. Nucleic acid detection methods using universal priming
US7157228B2 (en) * 2002-09-09 2007-01-02 Bioarray Solutions Ltd. Genetic analysis and authentication
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US20060183128A1 (en) 2003-08-12 2006-08-17 Epigenomics Ag Methods and compositions for differentiating tissues for cell types using epigenetic markers
EP1921156A1 (en) * 2006-11-10 2008-05-14 bioMerieux B.V. Improved multiplex nucleic acid amplification using blocked primers
AU2009214435C1 (en) * 2008-02-15 2014-07-17 Synthetic Genomics, Inc. Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
CN101935697B (zh) * 2010-04-16 2015-11-25 中生方政生物技术有限公司 用于核酸序列检测的方法和试剂盒
US20120052498A1 (en) * 2010-07-01 2012-03-01 Affymetrix, Inc. Detection of Nucleic Acids
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EP3447152A1 (en) * 2017-08-25 2019-02-27 Eawag, Swiss Federal Institute of Aquatic Science and Technology Accurate and massively parallel quantification of nucleic acid
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024005523A1 (ko) 2022-06-28 2024-01-04 주식회사 제노헬릭스 Rna 염기서열 분석을 위한 라이브러리 제작 방법
KR20240002713A (ko) 2022-06-28 2024-01-05 주식회사 제노헬릭스 Rna 염기서열 분석을 위한 라이브러리 제작 방법
KR20250033954A (ko) 2023-08-30 2025-03-10 주식회사 제노헬릭스 제한효소를 이용한 rna 라이브러리 제작 방법

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AU2019384154B2 (en) 2025-11-20
GB202308060D0 (en) 2023-07-12
GB2615955B (en) 2023-11-22
GB2615955A (en) 2023-08-23
EP3884047A1 (en) 2021-09-29
EP4644568A2 (en) 2025-11-05
GB202108738D0 (en) 2021-08-04
CN114901818A (zh) 2022-08-12
GB2595076A (en) 2021-11-17
GB2595076B8 (en) 2023-08-23
WO2020106906A1 (en) 2020-05-28
CA3120713A1 (en) 2020-05-28
US20210355485A1 (en) 2021-11-18
JP2022513124A (ja) 2022-02-07
GB2595076B (en) 2023-07-26
EP3884047A4 (en) 2022-08-17
AU2019384154A1 (en) 2021-06-10
EP3884047B1 (en) 2025-10-08
CN114901818B (zh) 2025-01-10
EP4644568A3 (en) 2025-12-03

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