JP2022513124A - 標的化された核酸ライブラリー形成のための方法 - Google Patents

標的化された核酸ライブラリー形成のための方法 Download PDF

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JP2022513124A
JP2022513124A JP2021529113A JP2021529113A JP2022513124A JP 2022513124 A JP2022513124 A JP 2022513124A JP 2021529113 A JP2021529113 A JP 2021529113A JP 2021529113 A JP2021529113 A JP 2021529113A JP 2022513124 A JP2022513124 A JP 2022513124A
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adapter
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nucleic acid
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ヘン ワン,
シェンロン リン,
グレイス ジャオ,
ユン バオ,
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    • C12Q1/6844Nucleic acid amplification reactions
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    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1068Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
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    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

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JP2021529113A 2018-11-21 2019-11-20 標的化された核酸ライブラリー形成のための方法 Pending JP2022513124A (ja)

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US201862770585P 2018-11-21 2018-11-21
US62/770,585 2018-11-21
PCT/US2019/062507 WO2020106906A1 (en) 2018-11-21 2019-11-20 Methods for targeted nucleic acid library formation

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JP2022513124A true JP2022513124A (ja) 2022-02-07
JP2022513124A5 JP2022513124A5 (https=) 2023-01-26

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US (1) US20210355485A1 (https=)
EP (2) EP4644568A3 (https=)
JP (1) JP2022513124A (https=)
KR (1) KR20210141915A (https=)
CN (1) CN114901818B (https=)
AU (1) AU2019384154B2 (https=)
CA (1) CA3120713A1 (https=)
GB (2) GB2615955B (https=)
WO (1) WO2020106906A1 (https=)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111254500B (zh) 2012-12-10 2024-01-23 分析生物科学有限公司 靶向基因组分析的方法
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
JP6913089B2 (ja) 2015-11-11 2021-08-04 レゾリューション バイオサイエンス, インコーポレイテッド Dnaライブラリーの高効率構築
US11319594B2 (en) 2016-08-25 2022-05-03 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
WO2022055984A1 (en) * 2020-09-08 2022-03-17 Resolution Bioscience, Inc. Adaptors and methods for high efficiency construction of genetic libraries and genetic analysis
ES2960777T3 (es) * 2021-03-18 2024-03-06 Genomill Health Oy Métodos para la cuantificación paralela precisa de ácidos nucleicos en muestras diluidas o no purificadas
EP4215619A1 (en) * 2022-01-21 2023-07-26 Genomill Health Oy Methods for sensitive and accurate parallel quantification of nucleic acids
US20250101412A1 (en) * 2022-01-25 2025-03-27 Oregon Health & Science University Methods and systems for increasing capture potential of a spatial array
KR20240150780A (ko) * 2022-02-18 2024-10-16 애질런트 테크놀로지스, 인크. 표적 핵산 포획 및 바코딩을 위한 시스템 및 방법
KR20250017217A (ko) * 2022-05-27 2025-02-04 쳉-야오 첸 핵산의 양쪽 표지를 위한 방법, 키트 및 시스템
KR20240002713A (ko) 2022-06-28 2024-01-05 주식회사 제노헬릭스 Rna 염기서열 분석을 위한 라이브러리 제작 방법
WO2024005523A1 (ko) 2022-06-28 2024-01-04 주식회사 제노헬릭스 Rna 염기서열 분석을 위한 라이브러리 제작 방법
KR20250033954A (ko) 2023-08-30 2025-03-10 주식회사 제노헬릭스 제한효소를 이용한 rna 라이브러리 제작 방법
WO2025221668A1 (en) * 2024-04-19 2025-10-23 Agilent Technologies, Inc. Methods of preparing nucleic acids for sequencing and methylation analysis
US12509733B1 (en) 2025-04-07 2025-12-30 Inivata Ltd. Methods and systems for detecting a primary disease

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180066306A1 (en) * 2016-08-10 2018-03-08 Cirina, Inc. Methods of analyzing nucleic acid fragments
JP2023512522A (ja) * 2020-01-31 2023-03-27 アヴィダ バイオメッド, インコーポレイテッド 標的化された核酸捕捉のためのシステムおよび方法

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100249110B1 (ko) * 1992-05-06 2000-04-01 다니엘 엘. 캐시앙 핵산 서열 증폭 방법, 이에 이용되는 조성물 및 키트
FR2724934B1 (fr) * 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique
WO2001057268A2 (en) * 2000-02-07 2001-08-09 Illumina, Inc. Nucleic acid detection methods using universal priming
US7157228B2 (en) * 2002-09-09 2007-01-02 Bioarray Solutions Ltd. Genetic analysis and authentication
ATE319861T1 (de) * 2002-10-30 2006-03-15 Pamgene Bv Verbesserte methoden zur erzeugung einer vielzahl von rna-kopien
US20060183128A1 (en) 2003-08-12 2006-08-17 Epigenomics Ag Methods and compositions for differentiating tissues for cell types using epigenetic markers
EP1921156A1 (en) * 2006-11-10 2008-05-14 bioMerieux B.V. Improved multiplex nucleic acid amplification using blocked primers
AU2009214435C1 (en) * 2008-02-15 2014-07-17 Synthetic Genomics, Inc. Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
CN101935697B (zh) * 2010-04-16 2015-11-25 中生方政生物技术有限公司 用于核酸序列检测的方法和试剂盒
US20120052498A1 (en) * 2010-07-01 2012-03-01 Affymetrix, Inc. Detection of Nucleic Acids
WO2016100976A2 (en) * 2014-12-19 2016-06-23 Apprise Bio, Inc. Methods for identifying multiple epitopes in selected sub-populations of cells
EP2847353B1 (en) * 2012-05-10 2022-01-19 The General Hospital Corporation Methods for determining a nucleotide sequence
US20150011396A1 (en) * 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
SI4056712T1 (sl) 2012-09-20 2024-10-30 The Chinese University Of Hong Kong Neinvazivno določanje metiloma tumorja iz plazme
CN103806111A (zh) * 2012-11-15 2014-05-21 深圳华大基因科技有限公司 高通量测序文库的构建方法及其应用
US9708658B2 (en) * 2013-03-19 2017-07-18 New England Biolabs, Inc. Enrichment of target sequences
US20160340740A1 (en) 2014-01-30 2016-11-24 The Regents Of The University Of California Methylation haplotyping for non-invasive diagnosis (monod)
WO2015159292A2 (en) 2014-04-14 2015-10-22 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. A method and kit for determining the tissue or cell origin of dna
TWI813141B (zh) 2014-07-18 2023-08-21 香港中文大學 Dna混合物中之組織甲基化模式分析
WO2016037361A1 (zh) * 2014-09-12 2016-03-17 深圳华大基因科技有限公司 试剂盒及其在核酸测序中的用途
WO2016093838A1 (en) * 2014-12-11 2016-06-16 New England Biolabs, Inc. Enrichment of target sequences
CN106103742B (zh) * 2015-01-06 2019-12-10 深圳市海普洛斯生物科技有限公司 一种富集循环肿瘤dna的方法和试剂
US20160215331A1 (en) * 2015-01-26 2016-07-28 Yale University Flexible and scalable genotyping-by-sequencing methods for population studies
WO2016189288A1 (en) * 2015-05-22 2016-12-01 Cambridge Epigenetix Ltd Nucleic acid sample enrichment
WO2017096322A1 (en) * 2015-12-03 2017-06-08 Accuragen Holdings Limited Methods and compositions for forming ligation products
CN109072300B (zh) 2015-12-17 2023-01-31 伊路敏纳公司 区分复杂生物样品中的甲基化水平
ES3022457T3 (en) 2016-12-21 2025-05-28 Univ California Deconvolution and detection of rare dna in plasma
US11091791B2 (en) * 2017-02-24 2021-08-17 Mgi Tech Co., Ltd. Methods for hybridization based hook ligation
JP7248368B2 (ja) * 2017-03-01 2023-03-29 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 高度に特異的な環状近接ライゲーションアッセイ
WO2018175779A1 (en) * 2017-03-22 2018-09-27 The Board Of Trustees Of The Leland Stanford Junior University Molecular profiling using proximity ligation-in situ hybridization
CN110832084B (zh) * 2017-04-17 2025-06-20 吉复生物科技有限公司 用于制备核酸文库的方法、组合物和试剂盒
EP3447152A1 (en) * 2017-08-25 2019-02-27 Eawag, Swiss Federal Institute of Aquatic Science and Technology Accurate and massively parallel quantification of nucleic acid
WO2020069350A1 (en) * 2018-09-27 2020-04-02 Grail, Inc. Methylation markers and targeted methylation probe panel

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180066306A1 (en) * 2016-08-10 2018-03-08 Cirina, Inc. Methods of analyzing nucleic acid fragments
JP2023512522A (ja) * 2020-01-31 2023-03-27 アヴィダ バイオメッド, インコーポレイテッド 標的化された核酸捕捉のためのシステムおよび方法

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AU2019384154B2 (en) 2025-11-20
GB202308060D0 (en) 2023-07-12
GB2615955B (en) 2023-11-22
GB2615955A (en) 2023-08-23
EP3884047A1 (en) 2021-09-29
EP4644568A2 (en) 2025-11-05
GB202108738D0 (en) 2021-08-04
CN114901818A (zh) 2022-08-12
GB2595076A (en) 2021-11-17
GB2595076B8 (en) 2023-08-23
WO2020106906A1 (en) 2020-05-28
CA3120713A1 (en) 2020-05-28
KR20210141915A (ko) 2021-11-23
US20210355485A1 (en) 2021-11-18
GB2595076B (en) 2023-07-26
EP3884047A4 (en) 2022-08-17
AU2019384154A1 (en) 2021-06-10
EP3884047B1 (en) 2025-10-08
CN114901818B (zh) 2025-01-10
EP4644568A3 (en) 2025-12-03

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