KR20210116951A - Manufacturing method of solid medium for water culture using corn stalk and by-product of sugar cane stalk, and grow-bag comprising the solid medium - Google Patents
Manufacturing method of solid medium for water culture using corn stalk and by-product of sugar cane stalk, and grow-bag comprising the solid medium Download PDFInfo
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Abstract
Description
본 발명은 옥수수대와 사탕수수 줄기박을 이용한 수경재배용 고체배지의 제조방법 및 상기 방법으로 제조된 고체배지를 포함하는 그로우백에 관한 것이다. The present invention relates to a method for producing a solid medium for hydroponics using cornstalk and sugarcane stem meal, and to a grow bag comprising the solid medium prepared by the method.
농작물을 재배하는 방식은 노지재배와 시설재배로 나누어진다. 노지재배는 기존의 흙에서 아무런 시설을 하지 않고 농작물을 재배하는 방식으로, 작물이 햇빛을 직접 받아 광합성을 활발하게 하여 성장이나 알곡이 충실하며, 당도가 높아 맛이 좋고, 다양한 환경 스트레스 및 병충해에 높은 내성을 갖는 특징이 있으나, 갑작스런 고온, 저온, 가뭄, 강풍, 해충 대발생등으로 인해 큰 피해를 입는 문제가 있다. The method of cultivating crops is divided into open field cultivation and facility cultivation. Outdoor cultivation is a method of cultivating crops without any facilities in the existing soil. The crops receive direct sunlight to activate photosynthesis, so growth and grains are enriched. It has high resistance, but there is a problem in that it suffers a lot of damage due to sudden high temperature, low temperature, drought, strong wind, pest outbreak, etc.
시설재배는 재해배환경을 작물생육에 알맞도록 인위적으로 조절하는 모든 종류의 재배양식을 포함하는 방식으로, 간단한 비닐하우스를 비롯하며, 바람막이, 터널, 유리온실 등의 시설을 이용하여 재배하는 방식을 말한다. 시설재배의 재배환경은 해충, 병, 잡초 등과 같은 생물적 환경과 빛, 온도, 습도, 양분등과 같은 무생물적 환경으로 구분 할 수 있으며, 이들 환경을 보완하거나 최적 상태로 조절함으로써 작물생산량을 크게 높일 수 있고, 수확기를 조절함으로써 연중 생산이 가능하여 높은 가격을 받을 수 있다는 장점이 있어 재배면적이 매년 증가하고 있다.Facility cultivation is a method that includes all types of cultivation methods that artificially control the recultivation environment to be suitable for crop growth. say The cultivation environment of facility cultivation can be divided into a biotic environment such as pests, diseases, weeds, etc. and an abiotic environment such as light, temperature, humidity, and nutrients. The cultivation area is increasing every year because it has the advantage of being able to raise the crop and receiving a high price because it can be produced year-round by adjusting the harvest season.
수경재배는 시설재배농업 중에서도 토양없이 양액만으로 재배하는 방식으로, 작물의 생육에 필요한 물과 양분을 토양으로 공급받는 것이 아니라 양분을 적당한 비율로 깨끗한 물에 용해시켜 배양액을 만든 후 이를 잘 흡수할 수 있도록 지하부 및 지상부의 환경을 인위적으로 조절하면서 식물을 재배하는 방법이다. 수경재배는 인구증가로 인한 식량부족, 재배면적의 감소, 고품질 농산물에 대한 수요 증가 등에 부응하여 기존 토양재배의 대안으로 등장하였으며, 무공해 고품질 생산물을 대량생산할 수 있다는 장점이 있어서 재배역사는 매우 짧지만 농업구조개선을 위한 국가예산의 투자확대로 재배면적의 증가, 작물의 다양화, 양액재배시스템 자재의 국산화, 재배시설의 현대화 등에서 많은 발전을 이루고 있다. 이러한 수경재배는 토양없이 양액만으로 재배한다고 해서 무토양재배(soilless culture), 양액재배(nutrient culture) 라고도 불리며, 양액을 담은 탱크나 재배상을 이용해서 재배한다는 뜻의 탱크농업(tank farming) 또는 베드농업(bed farming) 등으로 불리고 있다.Hydroponics is a method of cultivating only with nutrient solution without soil among facility cultivation agriculture. Instead of receiving the water and nutrients necessary for crop growth into the soil, hydroponics dissolves nutrients in clean water in an appropriate ratio to make a culture solution and then absorbs them well. It is a method of cultivating plants while artificially controlling the environment of the underground and above-ground parts. Hydroponics has emerged as an alternative to conventional soil cultivation in response to food shortages due to population growth, a decrease in cultivated area, and an increase in demand for high-quality agricultural products. Due to the expansion of investment in the national budget to improve the agricultural structure, many developments are being made in the area of cultivation, diversification of crops, localization of nutrient solution cultivation system materials, and modernization of cultivation facilities. This hydroponics is also called soilless culture or nutrient culture because it is grown only with nutrient solution without soil. It is also called bed farming.
수경재배는 뿌리전체를 배양액 속에 침지하여 재배하는 담액수경방식(Deep Flow Technique), 배양액을 필름처럼 얇게 뿌리사이로 흘려서 공급하는 박막수경방식(Nutrient Film Technique), 뿌리가 베드의 배양액 위에 있게 한 상태에서 배양액을 뿌리로 분사해서 재배하는 분무경방식(Aeroponics), 화학적으로 불활성화한 매질에서 식물을 재배하는 고형 배지경방식(Solid Medium, Culture)으로 나뉜다. Hydroponics is a deep flow technique in which the entire root is immersed in a culture medium, and a thin film technique in which the culture solution is thinly flowed through the roots like a film. It is divided into aeroponics, which is grown by spraying the culture solution into the roots, and a solid medium, culture where plants are grown in a chemically inactivated medium.
고형 배지경방식은 순수 수경 방식과 달리 배지 내 통기가 용이하여 뿌리의 산소공급율이 높고, 뿌리로 공급되는 수분의 양을 조절할 수 있으며, 비순환방식으로 시설비를 낮춤과 동시에 토양전염균의 전파를 막을 수 있고, 배지 내 수분이 풍부하여 펌프가 정지하여도 상당시간 피해가 없다는 장점이 있다.Unlike the pure hydroponics method, the solid medium culture method allows easy ventilation in the medium, so the oxygen supply rate to the roots is high, and the amount of water supplied to the roots can be controlled. It has the advantage that there is no damage for a considerable period of time even if the pump is stopped due to the abundance of moisture in the medium.
이러한 고형 배지경방식의 배지로는 가격이 저렴하고 취급이 용이하여 작물생육에 우수한 물리화학적 특성을 갖는 물질이 사용되며, 대표적인 배지로는 피트모스, 톱밥, 야자섬유, 목탄등을 이용한 천연유기물배지, 암면, 입상면, 필라이트, 질석, 모래, 자갈 등을 이용한 천연광물 배지 및 폴리에스테르매트, 폴리우레탄, 폴리페놀섬유등을 이용한 인공섬유 배지가 있다.Materials with excellent physicochemical properties are used for crop growth due to their low price and easy handling. Representative media include natural organic media using peat moss, sawdust, palm fiber, charcoal, etc. There are natural mineral media using rock wool, granular cotton, fillite, vermiculite, sand, and gravel, and artificial fiber media using polyester mat, polyurethane, and polyphenol fibers.
현재 전세계적으로 수경 재배용으로 사용되고 있는 배지로는 펄라이트, 암면, 혼합배지 등이 있으며, 이들 중 펄라이트가 32.4%, 암면이 43.7%로 대부분을 차지한다. 그러나 대부분의 암면재배가 비순환식으로 이루어지고 있어 여분의 배양액이 토양으로 스며들어 토양과 지하수를 오염시키고 있으며, 암면 자체가 부식이 되지 않기 때문에 제2의 오염원이 되는 환경문제를 야기한다. 따라서 이에 대비되는 새로운 환경 친화형 배지 개발이 필요하다. Currently, there are pearlite, rock wool, mixed media, etc. as media used for hydroponics worldwide, and among them, pearlite accounts for 32.4% and rock wool accounts for 43.7%. However, since most rock wool cultivation is carried out in a non-circulating system, the excess culture solution seeps into the soil and contaminates the soil and groundwater, and since the rock wool itself does not corrode, it causes environmental problems that become a second source of pollution. Therefore, it is necessary to develop a new environment-friendly medium in contrast to this.
중국에서는 농업용 잔유물(옥수수대, 사탕수수대, 볏짚, 밀짚 등)의 소각이 금지되어 있어 이를 재활용하는 방법이 절실하다. 이에 따라서, 본 발명자들은 옥수수대와 사탕수수 줄기박의 재활용에 대해 연구하던 중, 상기 옥수수대와 사탕수수 줄기박이 수경재배용 양액의 사용량을 최소화하면서도 식물의 생육증진 효능이 우수한 수경재배용 고체 배지로서 유용하게 이용될 수 있음을 확인하여 본 발명을 완성하였다. In China, incineration of agricultural residues (corn stalks, sugar cane stalks, rice straw, straw, etc.) is prohibited, so a method for recycling them is urgently needed. Accordingly, while the present inventors were studying the recycling of cornstalks and sugarcane stemmeal, the cornstalks and sugarcane stemmeal were useful as a solid medium for hydroponics with excellent plant growth promoting efficacy while minimizing the amount of nutrient solution for hydroponics. The present invention was completed by confirming that it can be used.
본 발명의 목적은 옥수수대와 사탕수수 줄기박을 이용한 수경재배용 고체배지의 제조방법, 상기 제조방법으로 제조한 고체배지를 포함하는 그로우백을 제공하는 데에 있다. An object of the present invention is to provide a method for producing a solid medium for hydroponics using cornstalks and sugarcane stem meal, and a grow bag comprising the solid medium prepared by the method.
본 발명은 옥수수대와 사탕수수 줄기박을 이용한 수경재배용 고체배지의 제조방법을 제공한다. The present invention provides a method for producing a solid medium for hydroponics using cornstalks and sugarcane stem meal.
바람직하게는, (제1단계) 옥수수대, 사탕수수 줄기박, 볏짚, 밀짚, 콩비지, 쌀막걸리 찌꺼기, 닭축분 및 돼지축분을 혼합하여 원료 혼합물을 제조하고, 바실러스 서브틸리스(Bacillus subtilis) 균체 및 아스퍼질러스 니거(Aspergillus niger) 균체를 물에 희석한 발효용 미생물 희석액을 제조하는 단계;Preferably, (first step) to prepare a raw material mixture by mixing corn stalk, sugar cane stem meal, rice straw, wheat straw, okara, rice makgeolli residue, chicken livestock meal and pig livestock meal, Bacillus subtilis bacteria And Aspergillus niger ( Aspergillus niger ) preparing a microbial dilution solution for fermentation by diluting the cells in water;
(제2단계) 원료 혼합물의 수분함량이 65~70 중량%가 되도록 발효용 미생물 희석액을 원료 혼합물에 첨가하여 발효를 진행하여 발효 배지를 얻는 단계; 및,(Second step) obtaining a fermentation medium by adding a microbial dilution solution for fermentation to the raw material mixture so that the moisture content of the raw material mixture is 65 to 70 wt%; and,
(제3단계) 상기 발효 배지를 멸균하고 10~30℃에 8~15일간 두어 숙성하는 단계;를 포함할 수 있다. (Third step) sterilizing the fermentation medium and aging the fermentation medium by placing it at 10 to 30° C. for 8 to 15 days.
상기 제1단계의 원료혼합물 내의 원료들은 건조된 상태인 것을 사용할 수 있다. The raw materials in the raw material mixture of the first step may be in a dried state.
상기 제1단계의 원료혼합물은 옥수수대 100 중량부 기준으로, 사탕수수 줄기박 50~200 중량부, 볏짚 50~200 중량부, 밀짚 50~200 중량부, 콩비지 5~20 중량부, 쌀막걸리 찌꺼기 5~20 중량부, 닭축분 5~20 중량부 및 돼지축분 5~20 중량부를 포함한 것일 수 있다. 이 중, 옥수수대, 사탕수수 줄기박, 볏짚, 밀짚은 10~20mm의 입자크기를 갖는 상태로 분쇄된 것일 수 있다.The raw material mixture of the first step is based on 100 parts by weight of cornstalk, 50-200 parts by weight of sugarcane stem meal, 50-200 parts by weight of rice straw, 50-200 parts by weight of wheat straw, 5-20 parts by weight of okara, rice makgeolli residue 5-20 parts by weight, 5-20 parts by weight of chicken stock, and 5-20 parts by weight of pork stock may be included. Among them, cornstalk, sugarcane stem meal, rice straw, and wheat straw may be pulverized to have a particle size of 10 to 20 mm.
상기 제1단계의 발효용 미생물 희석액은 물 100 중량부 기준 각각의 균체를 0.01~0.1 중량씩 혼합하여 제조할 수 있다. 상기 바실러스 서브틸리스 균체, 바실러스 애시도풀루리티커스, 아스퍼질러스 니거 균체는 발효식품용으로 판매되는 것은 어떤 것이든지 사용할 수 있다. 이들 균주는 전용 배지에서 배양된 배양액을 원심분리하여 얻은 생균을 사용할 수도 있으며, 시판 분말 종균제를 사용해도 무방하다. The microbial dilution solution for fermentation in the first step may be prepared by mixing 0.01 to 0.1 weight of each of the cells based on 100 parts by weight of water. As for the Bacillus subtilis cells, Bacillus acidopuluriticus, and Aspergillus niger cells, any that are sold for fermented food may be used. For these strains, live cells obtained by centrifugation of a culture medium cultured in a dedicated medium may be used, or a commercially available powdered spawning agent may be used.
상기 제2단계의 발효는 55~70℃에서 25~30일간 수행할 수 있다. 상기 제2단계의 55~70℃에서 25~30일간 발효를 통해 대장균을 비롯한 각종 유해세균이 없어지고, 선충의 알들도 사멸하여, 식물생장에 가장 적합한 상태의 고형배지를 제조할 수 있다.Fermentation of the second step may be performed at 55 to 70° C. for 25 to 30 days. Various harmful bacteria including E. coli are eliminated through fermentation at 55 to 70° C. for 25 to 30 days in the second step, and eggs of nematodes are also killed, so that a solid medium most suitable for plant growth can be prepared.
상기 제3단계의 멸균은 1.2~1.5기압, 120~130℃에서 15~30분간 수행하는 것이 좋다. The sterilization of the third step is preferably performed for 15 to 30 minutes at a pressure of 1.2 to 1.5 atmospheres and 120 to 130 ° C.
상기 3단계의 숙성 과정을 통해 각 원료 성분이 잘 결착되고, 오염원의 발생이 나타나지 않은 것을 확인하여 최종적으로 수경재배용 고체배지를 얻을 수 있다. Through the three-step aging process, it is confirmed that each raw material component is well bound and the generation of contaminants does not appear, so that a solid medium for hydroponics can be finally obtained.
이에 본 발명은 상기 방법으로 제조된 수경재배용 고체배지와, 상기 고체배지가 함유된 식물 재배용 그로우백을 제공할 수 있다. Accordingly, the present invention can provide a solid medium for hydroponics prepared by the above method, and a grow bag for plant cultivation containing the solid medium.
상기 식물 재배용 그로우백에 포함될 수 있는 수경재배용 양액은 식물에 따라 달라지지만, 통상적으로 이용되는 해당 식물용 수경재배용 양액은 어떤 것이든지 사용가능하다. The nutrient solution for hydroponics that can be included in the grow bag for plant cultivation varies depending on the plant, but any nutrient solution for hydroponics for the plant commonly used may be used.
본 발명은 옥수수대와 사탕수수 줄기박을 이용한 수경재배용 수경재배용 고체배지의 제조방법 및 상기 방법으로 제조된 그로우백에 관한 것으로서, 상기 방법을 통해 옥수수대와 사탕수수 줄기박, 각종 농축산 부산물/폐기물의 혼합 발효를 통해 다양한 식물의 생육증진 효능이 있는 수경재배용 수경재배용 고체배지와 이를 함유하는 그로우백의 제조가 가능하다. The present invention relates to a method for producing a solid medium for hydroponics for hydroponics using cornstalk and sugarcane stem meal, and a grow bag prepared by the method, and through the method, cornstalk and sugarcane stem meal, various agricultural by-products/wastes It is possible to produce a solid medium for hydroponic cultivation that has the effect of promoting the growth of various plants and a grow bag containing it through mixed fermentation of
옥수수는 중국의 동북지방을 중심으로 전국에서 재배되며, 사탕수수는 남방지방에서 주로 생산되는 중요한 식물로서, 옥수수대와 사탕수수대가 식품 부산물로 대량 생산된다. 이들은 식품 부산물로서 폐기되는 것들이지만, 중국에서는 농업용 잔유물 (옥수수대, 볏짚, 밀짚 등) 의 소각이 금지되어 있어 그 재활용 방법이 절실히 필요하다. 옥수수대와 사탕수수대는 단단하면서도 성긴 고유의 섬유조직을 갖고 있기에, 중국에 생산기반을 둘 경우 폐기되는 부산물인 옥수수대와 사탕수수대(사탕수수 줄기박), 각종 농축산 부산물/폐기물을 이용하여 수경재배용 고체 배지로 제조하고 이를 그로우백에 넣어 식물 종자의 파종이나 모종 이식을 할 경우, 소량의 수경재배용 양액만으로도 각종 식물의 재배를 용이하게 할 수 있다. Corn is cultivated all over the country centered on the northeast region of China, and sugar cane is an important plant mainly produced in the southern regions. Although these are discarded as food by-products, incineration of agricultural residues (corn stalks, rice straw, straw, etc.) is prohibited in China, so recycling methods are urgently needed. Since cornstalks and sugarcane stalks have their own hard and sparse fibrous structures, they are used for hydroponics using cornstalks and sugarcane stalks (sugar cane stem meal), which are by-products that are discarded when production is based in China, and various agricultural by-products/wastes. When preparing a solid medium and putting it in a grow bag to sow plant seeds or transplant seedlings, it is possible to facilitate the cultivation of various plants with only a small amount of nutrient solution for hydroponics.
이하 본 발명의 바람직한 실시예를 상세히 설명하기로 한다. 그러나, 본 발명은 여기서 설명되는 실시예에 한정되지 않고 다른 형태로 구체화될 수도 있다. 오히려, 여기서 소개되는 내용이 철저하고 완전해지도록, 당업자에게 본 발명의 사상을 충분히 전달하기 위해 제공하는 것이다. Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided so that this disclosure will be thorough and complete, and will fully convey the spirit of the invention to those skilled in the art.
<실시예 1. 옥수수대와 사탕수수 줄기박을 이용한 그로우백의 제조><Example 1. Preparation of a grow bag using cornstalk and sugarcane stem meal>
바실러스 서브틸리스(Bacillus subtilis) 균체 및 아스퍼질러스 니거(Aspergillus niger)를 식품용 종균 판매사로부터 구입하였다. 옥수수대, 볏짚, 밀짚은 옥수수, 벼, 밀을 재배하는 농가에서 얻었으며, 사탕수수 줄기박은 중국으로부터 받아 사용하였다. 콩비지와 쌀막걸리 찌꺼기는 두부공장, 쌀막걸리 양조장에서 수거하였으며, 닭축분, 돼지축분은 닭과 돼지 축사로부터 공급받았다. 옥수수대, 사탕수수 줄기박, 볏짚, 밀짚은 사방 1~2cm의 길이를 갖는 입자로 분쇄하여 사용하였다. 모든 원료는 수분함량 10~15 중량%로 건조된 것을 사용하였다. Bacillus subtilis ( Bacillus subtilis ) Cells and Aspergillus niger ( Aspergillus niger ) were purchased from a food spawn vendor. Corn stalks, rice straw, and straw were obtained from farms growing corn, rice, and wheat, and sugar cane stem meal was obtained from China. Soybean okara and rice makgeolli residues were collected from tofu factories and rice makgeolli breweries, and chicken and pig livestock were supplied from chicken and pig farms. Corn stalks, sugar cane stem meal, rice straw, and wheat straw were used by pulverizing them into particles having a length of 1 to 2 cm in all directions. All raw materials were dried to a moisture content of 10 to 15% by weight.
이렇게 준비된 옥수수대 100g, 사탕수수 줄기박 100g, 볏짚 100g, 밀짚 100g, 콩비지 10g, 쌀막걸리 찌꺼기 10g, 닭축분 10g, 돼지축분 10g을 혼합하여 원료 혼합물을 제조하였다. A raw material mixture was prepared by mixing 100 g of corn stalk, 100 g of sugarcane stem meal, 100 g of rice straw, 100 g of wheat straw, 10 g of okara, 10 g of rice makgeolli residue, 10 g of chicken meat meal, and 10 g of pig meat meal.
물 100g당 바실러스 서브틸리스 균체, 아스퍼질러스 니거 균체 각각을 0.05g을 혼합하여 미생물 희석액을 제조하였다. Bacillus subtilis cells and Aspergillus niger cells were each mixed with 0.05 g per 100 g of water to prepare a microbial dilution.
상기 원료 혼합물의 수분함량이 65~70 중량%가 되도록 발효용 미생물 희석액을 원료 혼합물에 첨가한 후, 55~70℃에서 25~30일간 발효를 수행하였다. 이 때, 수분함량 65 중량% 상태의 발효용 원료를 뚜껑이 있으면서 공기순환용 구멍이 있는 발효조에 넣고 수분함량이 유지되게 하였다. 이 후 발효조로부터 발효물을 꺼내어 1.5기압, 121℃에서 15분간 멸균하고, 10일간 25℃에 두어 숙성하였다. 숙성과정에서 혼합된 각 성분이 잘 결착되고, 오염원의 발생이 나타나지 않은 것을 확인하여 최종적으로 수경 재배용 고체 배지를 제조하였다. 이 후 식물재배용 그로우백에 상기 조성물을 넣고, 양액을 흘려보낼 수 있는 관을 삽입하였다. A microbial dilution solution for fermentation was added to the raw material mixture so that the moisture content of the raw material mixture was 65 to 70 wt %, and then fermentation was performed at 55 to 70 ° C. for 25 to 30 days. At this time, the raw material for fermentation with a moisture content of 65% by weight was put into a fermenter having a hole for air circulation while having a lid, and the moisture content was maintained. Thereafter, the fermented product was taken out of the fermenter, sterilized at 1.5 atm, 121°C for 15 minutes, and aged at 25°C for 10 days. It was confirmed that each component mixed in the aging process was well bound, and the generation of contaminants did not appear, and finally, a solid medium for hydroponics was prepared. After that, the composition was put into a grow bag for plant cultivation, and a tube through which the nutrient solution could flow was inserted.
<실시예 2. 배지 성분별 수경 재배용 고체 배지의 제조><Example 2. Preparation of a solid medium for hydroponic cultivation by medium component>
실시예 1과 동일하게 수경 재배용 고체 배지를 제조하되, 하기 표 1과 같이 각 원료의 함량을 달리하여 배지를 제조하였다. A solid medium for hydroponics was prepared in the same manner as in Example 1, but the medium was prepared by varying the content of each raw material as shown in Table 1 below.
비지 (g)bean
okara (g)
축분 (g)chicken
livestock meal (g)
축분 (g)pig
livestock meal (g)
<비교예 1. 비교군 수경 재배용 고체 배지의 제조 a ><Comparative Example 1. Preparation of a solid medium for hydroponic cultivation of comparative group a>
실시예 1과 동일하게 버섯 재배용 배지를 제조하되, 하기 표 2와 같이 각 원료의 함량을 달리하여 배지를 제조하였다. A medium for mushroom cultivation was prepared in the same manner as in Example 1, but the medium was prepared by varying the content of each raw material as shown in Table 2 below.
비지 (g)bean
okara (g)
축분 (g)chicken
livestock meal (g)
축분 (g)pig
livestock meal (g)
<비교예 2. 비교군 수경 재배용 고체 배지의 제조 b ><Comparative Example 2. Preparation of solid medium for hydroponic cultivation of comparative group b>
비교예 2-1Comparative Example 2-1
실시예 1과 동일한 방법으로 수경 재배용 고체 배지를 제조하되, 발효과정을 생략하고 물만 처리하여 수분함량을 65중량%가 된 상태로 바로 멸균하여 고체배지를 제조하였다. A solid medium for hydroponics was prepared in the same manner as in Example 1, except that the fermentation process was omitted and only water was treated, and the water content was immediately sterilized to 65% by weight to prepare a solid medium.
비교예 2-2 Comparative Example 2-2
실시예 1과 동일한 방법으로 수경 재배용 고체 배지를 제조하되, 발효균으로 바실러스 서브틸리스만 이용하였다. (미생물 균체의 무게는 같음, 이하 동일)A solid medium for hydroponics was prepared in the same manner as in Example 1, but only Bacillus subtilis was used as the fermenter. (The weight of the microbial cells is the same, hereinafter the same)
비교예 2-3 Comparative Example 2-3
실시예 1과 동일한 방법으로 수경 재배용 고체 배지를 제조하되, 발효균으로 아스퍼질러스 오리제를 이용하였다.A solid medium for hydroponics was prepared in the same manner as in Example 1, but Aspergillus oryzae was used as the fermenter.
비교예 2-4 Comparative Example 2-4
실시예 1과 동일한 방법으로 수경 재배용 고체 배지를 제조하되, 발효균으로 아스퍼질러스 니거만 이용하였다. A solid medium for hydroponics was prepared in the same manner as in Example 1, but only Aspergillus niger was used as the fermenter.
비교예 2-5 Comparative Example 2-5
실시예 1과 동일한 방법으로 수경 재배용 고체 배지를 제조하되, 옥수수대, 사탕수수 줄기박, 볏짚, 밀짚 대신 Empty Fruit Bunch(팜오일 추출공정의 부산물로 발생되는 셀룰로오스를 주성분으로 포함하는 부산물); 및 코코피트를 1:1의 중량비로 혼합한 것을 옥수수대, 사탕수수 줄기박, 볏짚, 밀짚의 혼합 중량과 동량으로 이용하였다. A solid medium for hydroponics was prepared in the same manner as in Example 1, but instead of cornstalk, sugarcane stem meal, rice straw, and straw, Empty Fruit Bunch (a by-product containing cellulose as a main component generated as a by-product of the palm oil extraction process); and coco peat in a weight ratio of 1:1 was used in the same amount as the mixed weight of corn stalk, sugar cane stem meal, rice straw, and wheat straw.
<실험예 1. 각 식물의 생육 촉진 효과 확인> <Experimental Example 1. Confirmation of the effect of promoting growth of each plant>
상추, 토마토, 오이고추, 딸기, 오이, 당귀, 케일, 가지의 모종을 구입하거나 키워 시판 고체배지를 넣은 그로우백, 실시예 1, 실시예 2, 비교예 1, 비교예 2의 고체배지를 넣은 그로우백에 옮겨심어 1개월간 키운 후 각 식물의 무게를 시판 고체배지가 포함된 그로우백에서 자란 무게를 100%로 환산하여 측정하였다. 이 실험은 각 식물이 생육하기 좋은 시기와 온도에 따라 개별로 실시하여 총 1년간 수집된 데이터를 하기와 같이 기록하였다. 이 때 사용한 각 양액은 식물별로 국립원예특작과학원에서 추천하는 양액을 사용하였다.Buy or grow seedlings of lettuce, tomato, cucumber and pepper, strawberry, cucumber, angelfish, kale, and eggplant, and put the grow bag in a commercially available solid medium, Examples 1, 2, Comparative Example 1, Comparative Example 2 The weight of each plant was measured by converting the weight of each plant into a grow bag containing a commercially available solid medium to 100% after transplanting it into a grow bag and growing it for 1 month. This experiment was conducted individually according to the best time and temperature for each plant to grow, and the data collected for a total of one year was recorded as follows. For each nutrient solution used at this time, the nutrient solution recommended by the National Institute of Horticultural and Herbal Science for each plant was used.
또한, 상기 양액을 100% 공급한 표 3의 시판배지 생육량을 100%를 기준으로 할 때, 각 양액 총 부피를 60% 모두 줄여 공급하면서 식물 모종의 1개월간 생육시 총 무게 증가율을 확인하였다. In addition, when the growth amount of the commercial medium in Table 3 in which 100% of the nutrient solution was supplied was 100%, the total volume of each nutrient solution was reduced by 60% and the total weight increase rate was confirmed during one month of growth of the plant seedlings.
식물 총 무게 증가율 (%)When the total volume of nutrient solution is reduced to 60% during one month growth of each plant seedling
Total plant weight gain (%)
시판배지
(양액 100%)of table 3
commercial badge
(Nurture solution 100%)
시판배지
(양액 60%)of table 3
commercial badge
(Nurture solution 60%)
양액 60%)(all below
Nutrient 60%)
그 결과, 표 3의 결과를 참고할 때, 시판용 고체배지와 비교하여 비교예 1과 비교예 2의 고체배지에서 자란 각 식물의 총 무게는 크게 차이나지 않거나 증가한다 하여도 20% 미만이었으나, 실시예 1 및 2의 배지에서 자란 식물은 30% 이상 더 성장하는 것으로 확인되어 식물 재배용 배지로서의 탁월함이 확인된다. As a result, when referring to the results in Table 3, the total weight of each plant grown in the solid medium of Comparative Example 1 and Comparative Example 2 compared to the commercially available solid medium did not differ significantly or even increased, but was less than 20%, Example 1 And plants grown in the medium of 2 were confirmed to grow more than 30%, confirming excellence as a medium for plant cultivation.
또한 표 4의 결과를 참고할 때, 양액 총량을 60%로 줄였을 때 비교 조건의 고체배지에서는 식물 생장 정도가 양액 100%일 때보다 더 낮은 것으로 나타나지만, 실시예 1 및 2의 배지는 식물 생장에 무리가 가지 않음을 확인할 수 있다. In addition, referring to the results in Table 4, when the total amount of nutrient solution was reduced to 60%, the degree of plant growth in the solid medium of the comparative condition was lower than that when the nutrient solution was 100%, but the medium of Examples 1 and 2 was used for plant growth. You can see that there is no problem.
Claims (6)
(제2단계) 원료 혼합물의 수분함량이 65~70 중량%가 되도록 발효용 미생물 희석액을 원료 혼합물에 첨가하여 발효를 진행하여 발효 배지를 얻는 단계; 및,
(제3단계) 상기 발효 배지를 멸균하고 10~30℃에 8~15일간 두어 숙성하는 단계;를 포함하는 것을 특징으로 하는 옥수수대와 사탕수수 줄기박을 이용한 수경재배용 고체배지의 제조방법.(Step 1) A raw material mixture is prepared by mixing corn stalk, sugarcane stem meal, rice straw, wheat straw, okara, rice makgeolli residue, chicken stock and pig stock, Bacillus subtilis bacteria and Aspergillus Niger ( Aspergillus niger ) preparing a microbial dilution solution for fermentation by diluting the cells in water;
(Second step) to obtain a fermentation medium by adding a microbial dilution solution for fermentation to the raw material mixture so that the moisture content of the raw material mixture is 65 to 70 wt%; and,
(Third step) A method for producing a solid medium for hydroponics using cornstalks and sugar cane stem meal, comprising the step of sterilizing the fermentation medium and leaving it to ripen for 8 to 15 days at 10 to 30°C.
상기 제1단계의 옥수수대, 사탕수수 줄기박, 볏짚, 밀짚은 10~20mm의 입자크기를 갖는 상태로 분쇄된 분쇄물인 것을 특징으로 하는 옥수수대와 사탕수수 줄기박을 이용한 수경재배용 고체배지의 제조방법.According to claim 1,
Preparation of a solid medium for hydroponics using cornstalk and sugarcane stem meal, characterized in that the cornstalk, sugarcane stem meal, rice straw, and wheat straw in the first step are pulverized products having a particle size of 10 to 20 mm Way.
상기 제2단계의 발효는 55~70℃에서 25~30일간 수행하는 것을 특징으로 하는 옥수수대와 사탕수수 줄기박을 이용한 수경재배용 고체배지의 제조방법.According to claim 1,
The method for producing a solid medium for hydroponics using cornstalk and sugarcane stem meal, characterized in that the fermentation of the second step is carried out at 55-70° C. for 25-30 days.
상기 제3단계의 멸균은 1.2~1.5기압, 120~130℃에서 15~30분간 수행하는 것을 특징으로 하는 옥수수대와 사탕수수 줄기박을 이용한 수경재배용 고체배지의 제조방법.According to claim 1,
The sterilization of the third step is a method for producing a solid medium for hydroponics using cornstalks and sugar cane stem meal, characterized in that it is performed at 1.2 to 1.5 atmospheres and 120 to 130° C. for 15 to 30 minutes.
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