KR20210065591A - Functional cosmetic composition comprising buah merah fruit oil and clove flower extract as active ingredients - Google Patents

Abstract

The present invention relates to a functional cosmetic composition, and more particularly, to a functional cosmetic composition containing a Pandanus conoideus fruit oil and a clove flower extract as active ingredients to have anti-inflammatory, antioxidant, atopy alleviation and acne alleviation effects by comprising a mixture in which a Pandanus conoideus fruit oil and a clove flower extract are mixed as active ingredients.

Description

Functional cosmetic composition comprising buah merah fruit oil and clove flower extract as active ingredients

The present invention relates to a functional cosmetic composition, and more particularly, to have an anti-inflammatory effect, antioxidant effect, atopy improvement effect and acne improvement effect by including a mixture of buamera fruit oil and clove flower extract as an active ingredient. It relates to a functional cosmetic composition comprising oil and clove flower extract as active ingredients.

Human skin is prone to various troubles. Generally, acne that occurs after puberty and atopic skin disease that begins in infancy are examples.

The acne is one of the inflammatory skin diseases in which comedones, papules, pus blisters, nodules, and pseudocysts occur mainly on the face, neck, chest, back, and shoulder areas. It can be severe and can also occur in adults in their 30s and 40s. As such, acne is a disease that appears regardless of gender and age.

If acne is left unattended and the inflammation worsens, a pitted scar is formed, and if the sebum bursts inside the skin by squeezing it incorrectly, the skin will protrude unevenly. Scars caused by acne have an adverse effect on skin beauty.

On the other hand, atopic skin disease is a chronic recurrent inflammatory skin disease that mainly begins in infancy or childhood, and is accompanied by pruritus, dry skin, and characteristic eczema. In infancy, it begins as eczema on the face and on the extensor side of the limbs, but in childhood, it characteristically shows eczema on the bent part of the arm and the bent part behind the knee. Although the cause of atopic skin disease has not been clearly identified, environmental factors, genetic factors, immunological abnormalities, and abnormal skin barriers are expected to be the main causes.

Atopic dermatitis often improves or disappears with age, but even after improvement, itching or inflammatory reaction tends to occur easily due to specific substances or stimuli, and hand and foot eczema often appear in childhood and adulthood. If atopic skin disease remains until adulthood, erythema on the face tends to appear as severe eczema, which adversely affects skin beauty.

Numerous studies and efforts have been focused on treating acne and atopic skin diseases as described above, and accordingly, various therapeutic compositions containing chemicals as active ingredients have appeared.

However, a therapeutic composition containing a chemical as an active ingredient has frequent side effects and has a problem that it is not ecologically sustainable, so there is a problem that the production cost is high, and there is a problem that it is difficult for users to easily access it due to the high cost.

Therefore, as a new material that is ecologically sustainable and safe, a composition using a natural material is attracting attention.

Republic of Korea Patent Publication No. 10-1666151 (announced on October 17, 2016), "Chemical extract by ultrasonic extraction method, manufacturing method thereof, and cosmetic composition for antioxidative, anti-inflammatory and atopic skin improvement using raw herb extract as an active ingredient"

In order to solve the above problems, the present invention contains a mixture of buamera fruit oil and clove flower extract as an active ingredient to have anti-inflammatory effect, antioxidant effect, atopy improvement effect and acne improvement effect. An object of the present invention is to provide a functional cosmetic composition comprising a flower extract as an active ingredient.

In order to solve the above problems, the present invention provides a functional cosmetic composition comprising buamera fruit oil and clove flower extract as active ingredients, characterized in that it contains a mixture of buamera fruit oil and clove flower extract as active ingredients provides

In addition, the buamera fruit oil and clove flower extract are mixed in a weight ratio of 30: 70 to 70: 30.

In addition, the boamera fruit oil is extracted using a steam distillation method, and the clove flower extract is characterized in that it is extracted with hot water at a temperature of 100 ℃ or less.

In addition, the mixture of the buamera fruit oil and clove flower extract is characterized in that it is contained in an amount of 0.1 to 10.0% by weight based on the total weight.

In addition, the cosmetic composition is characterized in that it has an anti-inflammatory effect, an antioxidant effect, an atopic improvement effect, and an acne improvement effect.

The functional cosmetic composition comprising Buamera fruit oil and clove flower extract as active ingredients according to the present invention contains a mixture of Buamera fruit oil and clove flower extract as an active ingredient, and has anti-inflammatory effect, antioxidant effect, atopy improvement effect and It has the effect of improving acne.

1 to 4 are graphs showing the anti-inflammatory effect of a functional cosmetic composition comprising buamera fruit oil and clove flower extract as active ingredients according to the present invention.
5 to 6 are graphs showing the antioxidant effect of the functional cosmetic composition comprising buamera fruit oil and clove flower extract as active ingredients according to the present invention.
7 to 8 are graphs showing the atopy improvement effect of the functional cosmetic composition comprising buamera fruit oil and clove flower extract as active ingredients according to the present invention.
9 is a graph showing the acne improvement effect of the functional cosmetic composition comprising buamera fruit oil and clove flower extract as active ingredients according to the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The following detailed description of the present invention is an embodiment in which the present invention may be practiced, and reference is made to the accompanying drawings shown as examples of the embodiments. These embodiments are described in sufficient detail to enable those skilled in the art to practice the present invention. It should be understood that various embodiments of the present invention are different but need not be mutually exclusive. For example, certain shapes, structures, and characteristics described herein may be implemented in other embodiments without departing from the spirit and scope of the invention in relation to one embodiment. In addition, it should be understood that the position or arrangement of individual components in each described embodiment may be changed without departing from the spirit and scope of the present invention.

Accordingly, the detailed description set forth below is not intended to be taken in a limiting sense, and the scope of the present invention, if properly described, is limited only by the appended claims, along with all scopes equivalent to those claimed by the claims. Like reference numerals in the drawings refer to the same or similar functions throughout the various aspects.

The terms used in the present invention have been selected as currently widely used general terms as possible while considering the functions in the present invention, which may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like. In addition, in specific cases, there are also terms arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than the name of a simple term.

In the present invention, when a part "includes" a certain component, this means that other components may be further included, rather than excluding other components, unless otherwise stated.

Hereinafter, the functional cosmetic composition comprising buamera fruit oil and clove flower extract according to the present invention as active ingredients will be described in detail.

The functional cosmetic composition comprising Buamera fruit oil and clove flower extract as active ingredients according to the present invention is 30:70 of boamera fruit oil extracted using steam distillation method and clove flower extract extracted with hot water at a temperature of 100°C or less. ~ 70: By including the mixture mixed in a weight ratio of 30 to 0.1 to 10.0% by weight based on the total weight, it has an anti-inflammatory effect, an antioxidant effect, atopic improvement effect and acne improvement effect.

The bouamera fruit oil refers to the oil extracted from the boamera fruit.

The boamera fruit is a fruit of the boamera ( Pandanus conoideus ). The boamera is a plant belonging to the Pandanaceae family, native to the island of New Guinea, and bears oval-shaped fruits with buds covered with leaves. The buamera fruit has a length of 55 cm, a diameter of 10 to 15 cm, and a weight of 2 to 3 kg. The boamera is known to be effective in degenerative diseases such as hypertension, diabetes, and heart disease, and is also known to be good for brain disease and frontal lobe development. Indigenous tribes in Indonesia use the buamera berries as firewood or extract oil for medicinal purposes.

The bouamera fruit oil is extracted from the bouamera fruit using the steam extraction method. For boamera fruit, either fresh or dried fruit can be used. This may be changed according to the practice of one of ordinary skill in the art. The bouamera fruit oil can be obtained by heating purified water to generate water vapor, eluting oil from the bouamera fruit while passing the water vapor between the bouamera fruit, and cooling the water vapor containing the oil with a cooler.

The clove flower extract ( Eugenia Caryophyllus ( Clove ) Flower Extract ) refers to an extract extracted from clove flowers.

The clove flower is a flower of clove ( Eugenia Caryophyllus ). The clove is a deciduous tree belonging to the family Moxae, about 10 m tall, and the leaves are oval-shaped, pointed and opposite, have spots, and the back side is curved. Light purple four-leaf flowers bloom in clusters at the ends of branches. The fruit is fragrant, and the fruit is a drupe, 2cm long, oval, and contains one seed. It is native to the Moluccas Islands in Southeast Asia and is widely cultivated in Africa. Pickled and dried red flower buds are called 'cloves' and are used as raw materials for medicinal herbs and sperm oil.

The clove flower extract is obtained by extracting clove flowers using hot water extraction. An extract can be obtained from the clove flower by putting the clove flower in a solvent and heating it to 100°C or less. It is appropriate that the temperature in the extraction is 100° C. or less, and when it exceeds this, the anti-inflammatory effect, antioxidant effect, atopy improvement effect and acne of the functional cosmetic composition comprising the buamera fruit oil and clove flower extract according to the present invention as active ingredients The improvement effect may not appear properly. It will be appropriate that the solvent for extracting the extract from the clove flower is purified water. However, the extraction solvent is not limited to purified water, and may be changed according to the practice of those skilled in the art. The extract extracted from the clove flower is separated by a centrifuge, and only the supernatant is taken and used.

The buamera fruit oil and clove flower extract are mixed in a weight ratio of 30: 70 to 70: 30. Most suitably, it would be a 50:50 mixture of buamera fruit oil and clove flower extract. When the mixing ratio of buamera fruit oil and clove flower extract is out of 30: 70 to 70: 30, the anti-inflammatory effect and antioxidant effect of the functional cosmetic composition comprising the buamera fruit oil and clove flower extract as active ingredients according to the present invention , there is a problem in that it is difficult to properly show atopic improvement effect and acne improvement effect. The mixing of the buamera fruit oil and clove flower extract can be made by stirring at a speed of 30 to 200 rpm through a stirrer at a temperature of 30 to 90 ° C. At this time, in order to facilitate mixing, natural origin such as lecithin, lanolin, saponin of surfactants, etc. may be included.

The mixture of the buamera fruit oil and clove flower extract is preferably included in an amount of 0.1 to 10.0% by weight based on the total weight of the cosmetic composition. When out of the range, there is a problem that the anti-inflammatory effect, antioxidant effect, atopy improvement effect and acne improvement effect of the functional cosmetic composition comprising the buamera fruit oil and clove flower extract according to the present invention as active ingredients do not appear clearly.

Hereinafter, through the following Examples, Comparative Examples, and Experimental Examples, the effect of the functional cosmetic composition comprising buamera fruit oil and clove flower extract according to the present invention as active ingredients will be described in detail.

Example One. Boamera fruit oil and clove flower extract Preparation of a functional cosmetic composition comprising an active ingredient

A functional cosmetic composition comprising the buamera fruit oil and clove flower extract of Example 1 as active ingredients was prepared through the following preparation method.

Step S10: Purified water was heated to generate water vapor, and oil was eluted from the bouamera fruit while passing water vapor through the boamera fruit, and the water vapor containing the oil was cooled with a cooler to prepare bouamera fruit oil.

Step S20: The clove flower was put in a solvent, heated to 100° C. or less, separated by a centrifuge, and only the supernatant was taken to prepare a clove flower extract.

Step S30: A mixture was prepared by mixing buamera fruit oil and clove flower extract in a weight ratio of 50:50.

Step S40: The mixture was diluted in purified water to 5.0% by weight to complete the experimental preparation.

Example 2 to 3. Boamera fruit oil and clove flower extract Functionality included as an active ingredient cosmetics Preparation of the composition

A composition was prepared according to the manufacturing process of Example 1, but in step S30, buamera fruit oil and clove flower extract were mixed at different mixing ratios, and the buamera fruit oil and clove flower extract of Examples 2 to 3 were used as active ingredients. A functional cosmetic composition comprising

Content of boamera fruit oil Content of clove flower extract Example 2 30% by weight 70% by weight Example 3 70% by weight 30% by weight

Example 4 to 7. Boamera fruit oil and clove flower extract Functionality included as an active ingredient cosmetics Preparation of the composition

A composition was prepared according to the manufacturing process of Example 1, but the ratio at which the mixture was diluted in purified water in step S40 was changed, respectively, and the functional cosmetic composition comprising the buamera fruit oil and clove flower extract of Examples 4 to 7 as active ingredients was prepared.

The ratio at which the mixture is diluted in purified water Example 4 0.1% by weight Example 5 3.0 wt% Example 6 7.0 wt% Example 7 10.0 wt%

comparative example One. clove flower extract containing cosmetics Preparation of the composition

Through the following preparation method, the cosmetic composition of Comparative Example 1 containing only the clove flower extract without including the buamera fruit oil was prepared.

Step S10: The clove flower was put in a solvent, heated to 100° C. or less, separated by a centrifuge, and only the supernatant was taken to prepare a clove flower extract.

Step S20: Experimental preparation was completed by diluting the clove flower extract in purified water to 5.0% by weight.

comparative example 2. Boamera fruit oil containing cosmetics Preparation of the composition

Through the following preparation method, the cosmetic composition of Comparative Example 2 was prepared which did not contain clove flower extract and contained only buamera fruit oil.

Step S10: Purified water was heated to generate water vapor, and oil was eluted from the bouamera fruit while passing the water vapor through the bouamera fruit, and the water vapor containing the oil was cooled with a cooler to prepare bouamera fruit oil.

Step S20: Experimental preparation was completed by diluting Buamera fruit oil in purified water to 5.0% by weight.

comparative example 3. Boamera fruit oil and clove flower extract by 10:90 mixed mixture containing cosmetics Preparation of the composition

The cosmetic composition of Comparative Example 3 was prepared through the following preparation method.

Step S10: Purified water was heated to generate water vapor, and oil was eluted from the bouamera fruit while passing the water vapor through the bouamera fruit, and the water vapor containing the oil was cooled with a cooler to prepare bouamera fruit oil.

Step S20: The clove flower was put in a solvent, heated to 100° C. or less, separated by a centrifuge, and only the supernatant was taken to prepare a clove flower extract.

Step S30: A mixture was prepared by mixing buamera fruit oil and clove flower extract in a weight ratio of 10:90.

Step S40: The mixture was diluted in purified water to 5.0% by weight to complete the experimental preparation.

comparative example 4. Boamera fruit oil and clove flower extract by 90:10 mixed mixture containing cosmetics Preparation of the composition

The cosmetic composition of Comparative Example 4 was prepared through the following preparation method.

Step S10: Purified water was heated to generate water vapor, and oil was eluted from the bouamera fruit while passing the water vapor through the bouamera fruit, and the water vapor containing the oil was cooled with a cooler to prepare bouamera fruit oil.

Step S20: Clove flower was added to a solvent, heated to 100° C. or lower, separated by centrifugation, and only the supernatant was taken to prepare a clove flower extract.

Step S30: A mixture was prepared by mixing buamera fruit oil and clove flower extract in a weight ratio of 90:10.

Step S40: The mixture was diluted in purified water to 5.0% by weight to complete the experimental preparation.

comparative example 5. Boamera fruit oil and clove flower extract If the mixture is 0.01 weight% included cosmetics Preparation of the composition

The cosmetic composition of Comparative Example 5 was prepared through the following preparation method.

Step S10: Purified water was heated to generate water vapor, and oil was eluted from the bouamera fruit while passing water vapor through the boamera fruit, and the water vapor containing the oil was cooled with a cooler to prepare bouamera fruit oil.

Step S20: Clove flower was added to a solvent, heated to 100° C. or lower, separated by centrifugation, and only the supernatant was taken to prepare a clove flower extract.

Step S30: A mixture was prepared by mixing buamera fruit oil and clove flower extract in a weight ratio of 50:50.

Step S40: The mixture was diluted in purified water to 0.01 wt% to complete the experimental preparation.

comparative example 6. Boamera fruit oil and clove flower extract 15.0 of the mixture weight% included cosmetics Preparation of the composition

The cosmetic composition of Comparative Example 6 was prepared through the following preparation method.

Step S10: Purified water was heated to generate water vapor, and oil was eluted from the bouamera fruit while passing water vapor through the boamera fruit, and the water vapor containing the oil was cooled with a cooler to prepare bouamera fruit oil.

Step S20: Clove flower was added to a solvent, heated to 100° C. or lower, separated by centrifugation, and only the supernatant was taken to prepare a clove flower extract.

Step S30: A mixture was prepared by mixing buamera fruit oil and clove flower extract in a weight ratio of 50:50.

Step S40: The mixture was diluted in purified water to 15.0 wt% to complete the experimental preparation.

Experimental example 1. Anti-inflammatory effect test

The anti-inflammatory effect experiment was conducted according to a known method. The mouse macrophage line, RAW264.7 cells, was treated with 100 units/ml penicillin-sterptomycin and 10% Fetal bovine serum (FBS) in DMEM (Dulbecco's Modified Eagle's Medium) medium (Gibco, Grand Island, NY, USA) at 37°C. , in a 5% CO₂ incubator (2 x 10 ⁵ cells/ml) in a 24-well plate for 24 hours.

Samples of Examples 1 to 7 and Comparative Examples 1 to 6 were diluted with DMSO solvent at each concentration of 12.5 ug/ml, 25 ug/ml, and 50 ug/ml, respectively, and treated for 1 hour at the same concentration, then LPS 1 ug/ml was treated for 24 hours. After 24 hours, the supernatant of the cell treatment solution was collected and the expression of TNF-α and IL-6 was confirmed by immunoassay.

In Equation 1, the absorbance of the untreated group (control group) means an average value calculated by measuring the absorbance 10 times for the same untreated group (control group).

Mixing weight ratio of boamera fruit oil (weight %) Mixing weight ratio of clove flower extract (weight %) TNF-alpha expression rate (%) 12.5ug/ml 25ug/ml 50ug/ml unprocessed - - -2.8 Control (LPS)
1ug/ml - - 100 Comparative Example 1 0 100 93.4 92.6 96.1 Comparative Example 3 10 90 78.9 83.4 79.4 Example 2 30 70 16.2 18.3 17.8 Example 1 50 50 12.2 15.6 13.7 Example 3 70 30 15.1 17.0 16.6 Comparative Example 4 90 10 55.4 54.4 52.6 Comparative Example 2 100 0 58.9 57.1 62.5 positive control - - -3.09

Ratio of mixture diluted in purified water (%) TNF-alpha expression rate (%) 12.5ug/ml 25ug/ml 50ug/ml unprocessed - -2.8 Control (LPS)
1ug/ml - 100 Comparative Example 5 0.01 95.1 97.3 89.9 Example 4 0.1 15.2 19.2 18.8 Example 5 3.0 14.7 17.1 18.2 Example 1 5.0 12.2 15.6 13.7 Example 6 7.0 13.8 16.1 14.2 Example 7 10.0 14.6 17.3 15.2 Comparative Example 6 15.0 55.1 45.6 56.5 positive control - -3.09

Mixing weight ratio of boamera fruit oil (weight %) Mixing weight ratio of clove flower extract (weight %) IL-6 expression rate (%) 12.5ug/ml 25ug/ml 50ug/ml unprocessed - - -23.21 Control (LPS)
1ug/ml - - 100 Comparative Example 1 0 100 92.3 97.3 95.1 Comparative Example 3 10 90 84.2 88.9 82.3 Example 2 30 70 34.6 25.9 20.9 Example 1 50 50 33.4 20.2 15.4 Example 3 70 30 35.3 23.5 18.6 Comparative Example 4 90 10 49.7 44.2 38.2 Comparative Example 2 100 0 57.1 55.8 59.2

Ratio of mixture diluted in purified water (%) IL-6 expression rate (%) 12.5ug/ml 25ug/ml 50ug/ml unprocessed - -23.21 Control (LPS)
1ug/ml - 100 Comparative Example 5 0.01 93.7 90.7 85.2 Example 4 0.1 31.4 30.3 35.4 Example 5 3.0 35.2 27.8 24.3 Example 1 5.0 33.4 20.2 15.4 Example 6 7.0 34.3 24.5 22.7 Example 7 10.0 37.2 36.3 34.3 Comparative Example 6 15.0 67.3 63.5 57.8

As can be seen from Tables 3 to 6, it was confirmed that the mixtures of Examples 1 to 7 of the present invention significantly inhibited TNF-α and IL-6, which were increased due to LPS treatment, respectively.

In particular, it was confirmed that when the boamera fruit oil and clove flower extract were mixed in a weight ratio of 30: 70 to 70: 30, and the mixture was included in an amount of 0.1 to 10.0% by weight based on the total weight, it was confirmed that it had an excellent anti-inflammatory effect.

Experimental example 2. Free radical scavenging effect experiment

The antioxidant effect test confirmed the antioxidant effect on the cosmetic compositions of Examples 1 to 7 and Comparative Examples 1 to 6 through the free radical scavenging effect using the DPPH method according to a known method.

After dispensing 100 μl of the cosmetic compositions of Examples 1 to 7 and Comparative Examples 1 to 6 into 96 microplates, 10 μl of a 2,2-diphenyl-1-picrylhydrazyl (DPPH) solution dissolved in ethanol to 1 mM was added. did. After 30 minutes of reaction at room temperature, absorbance was measured at 517 nm to measure free radical scavenging activity. Vitamin C at a concentration of 0.1% was used as a control group and tested with the cosmetic compositions of Examples 1 to 7 and Comparative Examples 1 to 6. The results are shown in Tables 3 and 4 based on the free radical scavenging effect of vitamin C as 100%. Table 3 shows the experimental results according to the mixing ratio of buamera fruit oil and clove flower extract, and Table 4 shows the experimental results according to the concentration containing the buamera fruit oil and clove flower extract mixed extract.

Mixing weight ratio of boamera fruit oil (weight %) Mixing weight ratio of clove flower extract (weight %) Free radical scavenging effect (%) Control group (vitamin C) - - 100% Comparative Example 1 0 100 24.2% Comparative Example 3 10 90 83.8% Example 2 30 70 93.2% Example 1 50 50 97.6% Example 3 70 30 92.3% Comparative Example 4 90 10 84.1% Comparative Example 2 100 0 64.2%

Ratio of mixed extract diluted in purified water (% by weight) Free radical scavenging effect (%) Control group (vitamin C) - 100% Comparative Example 5 0.01 36.3% Example 4 0.1 94.9% Example 5 3.0 96.3% Example 1 5.0 97.6% Example 6 7.0 96.8% Example 7 10.0 96.2% Comparative Example 6 15.0 88.4%

As a result, it was confirmed that when the boamera fruit oil and clove flower extract were mixed in a weight ratio of 30: 70 to 70: 30, and the mixture was included in an amount of 0.1 to 10.0 wt % based on the total weight, an excellent free radical scavenging effect was exhibited. .

Experimental example 3. Atopy improvement effect

Examples 1 to 7 and Comparative Examples 1 to 6 were tested for the improvement of atopy effect on the cosmetic composition.

Atopic improvement effect was investigated for 20 patients aged 5-30 years with atopic dermatitis who had suffered from atopic dermatitis for more than 2 years. The improvement effect was evaluated by a five-point scale method, and it was expressed as an average value of the values evaluated according to the degree of erythema, edema, exudation, skin peeling, and dryness, respectively.

Mixing weight ratio of boamera fruit oil (weight %) Mixing weight ratio of clove flower extract (weight %) Atopy improvement effect Comparative Example 1 0 100 1.2 Comparative Example 3 10 90 2.6 Example 2 30 70 4.6 Example 1 50 50 4.8 Example 3 70 30 4.6 Comparative Example 4 90 10 2.8 Comparative Example 2 100 0 1.4

Ratio of mixed extract diluted in purified water (% by weight) Atopy improvement effect Comparative Example 5 0.01 2.4 Example 4 0.1 4.2 Example 5 3.0 4.4 Example 1 5.0 4.8 Example 6 7.0 4.6 Example 7 10.0 4.4 Comparative Example 6 15.0 2.8

As a result, it was confirmed that when the boamera fruit oil and clove flower extract were mixed in a weight ratio of 30: 70 to 70: 30, and the mixture was included in an amount of 0.1 to 10.0% by weight relative to the total weight, an excellent atopy improvement effect was exhibited.

Experimental example 4. Acne improvement effect

In order to further prove the antibacterial activity of the cosmetic compositions of Examples 1 to 7 and Comparative Examples 1 to 6 to the acne-causing bacteria, MIC (minimal inhibitory concentration, minimum inhibitory concentration) was measured for the acne-causing bacteria.

Experimental raw materials were added to the sterilized Reinforced Clostridial Broth by sterilization and filtration at appropriate concentrations, and after inoculating 1% of each experimental group with the activated acne strain culture medium, Examples 1 to 7 and Comparative Examples 1 to 6 After putting the cosmetic composition at each concentration, it was _{anaerobically cultured for 72 hours at 37° C. under 10% CO 2} conditions, and 100 μl of each was dispensed into Reinforced Clostridial Agar, followed by anaerobic culture to confirm the growth of acne strains. In order to confirm the result obtained, the above experiment was repeated three times.

Mixing weight ratio of boamera fruit oil (weight %) Mixing weight ratio of clove flower extract (weight %) Minimum inhibitory concentration MIC (%) Comparative Example 1 0 100 24.3 Comparative Example 3 10 90 1.3 Example 2 30 70 0.8 Example 1 50 50 0.6 Example 3 70 30 0.7 Comparative Example 4 90 10 1.1 Comparative Example 2 100 0 15.7

As a result, it was confirmed that when buamera fruit oil and clove flower extract were mixed in a weight ratio of 30: 70 to 70: 30, an excellent acne improvement effect was exhibited.

conclusion.

Through the above Experimental Examples 1 to 4, the effect of the functional cosmetic composition comprising the buamera fruit oil and clove flower extract according to the present invention as active ingredients was confirmed.

The functional cosmetic composition comprising buamera fruit oil and clove flower extract according to the present invention as active ingredients suppressed gene expression of TNF-α and IL-6, and through this, excellent anti-inflammatory effect could be inferred.

The functional cosmetic composition comprising buamera fruit oil and clove flower extract according to the present invention as active ingredients has a free radical scavenging effect, and through this, excellent antioxidant effect could be inferred.

It was confirmed that the functional cosmetic composition comprising buamera fruit oil and clove flower extract according to the present invention as active ingredients had an excellent atopy improvement effect.

The functional cosmetic composition comprising buamera fruit oil and clove flower extract according to the present invention as active ingredients has an inhibitory effect on acne causative bacteria, and through this, an excellent acne improvement effect can be inferred.

In particular, the anti-inflammatory effect, antioxidant effect, atopy improvement effect, acne improvement of the functional cosmetic composition comprising the bouamera fruit oil and clove flower extract according to the present invention as an active ingredient than when using the clove flower extract alone As the effect was outstanding, it was confirmed that an unexpected effect occurred with the combination between Buamera fruit oil and clove flower extract. , it was confirmed that the antioxidant effect, the atopic improvement effect, and the acne improvement effect were significantly increased.

Accordingly, the present invention specifies that a functional cosmetic composition comprising buamera fruit oil and clove flower extract as active ingredients has been developed as a novel cosmetic composition having anti-inflammatory effect, antioxidant effect, atopic improvement effect, and acne improvement effect.

Although the present invention has been described in conjunction with the accompanying drawings, this is only one embodiment of various embodiments including the gist of the present invention, and is intended to be easily implemented by those of ordinary skill in the art. For the purpose, it is clear that the present invention is not limited to the embodiments described above. Accordingly, the protection scope of the present invention should be interpreted by the following claims, and all technical ideas within the equivalent range by changes, substitutions, substitutions, etc. within the scope not departing from the gist of the present invention are in the right of the present invention. will be included In addition, it is clarified that some components of the drawings are provided in an exaggerated or reduced form than in reality to more clearly describe the components.

Claims (5)

A functional cosmetic composition comprising, as an active ingredient, a mixture of buamera fruit oil and clove flower extract as an active ingredient.
According to claim 1,
The functional cosmetic composition comprising the bouamera fruit oil and clove flower extract as active ingredients, characterized in that the bouamera fruit oil and clove flower extract are mixed in a weight ratio of 30: 70 to 70: 30.
According to claim 1,
The functional cosmetic composition comprising the bouamera fruit oil and clove flower extract as active ingredients, wherein the bouamera fruit oil is extracted using a steam distillation method, and the clove flower extract is extracted with hot water at a temperature of 100° C. or less.
According to claim 1,
A functional cosmetic composition comprising buamera fruit oil and clove flower extract as active ingredients, characterized in that the mixture of buamera fruit oil and clove flower extract is contained in an amount of 0.1 to 10.0% by weight based on the total weight.
According to claim 1,
The cosmetic composition is a functional cosmetic composition comprising buamera fruit oil and clove flower extract as active ingredients, characterized in that it has an anti-inflammatory effect, antioxidant effect, atopic improvement effect and acne improvement effect.

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