KR20210056714A - Cosmetic Composition Containing extract of Pleurotus citrinopileatus and Broussonetia kazinoki branch for anti-aging - Google Patents

Abstract

The present invention relates to a composition having anti-aging activity comprising a mixed extract of Pleurotus cornucopiae extracts and Broussonetia kazinoki branch extracts. The mixed extract of Pleurotus cornucopiae extracts and Broussonetia kazinoki branch extracts of the present invention has no cytotoxicity and has an excellent cellular aging inhibitory effect, thereby being easily used in cosmetics, pharmaceuticals, and the like.

Description

A composition for anti-aging containing a mixed extract of Pleurotus citrinopileatus and Broussonetia kazinoki branch for anti-aging} as an active ingredient.

The present invention relates to a composition having anti-aging activity comprising a mixed extract of a yellow oyster mushroom extract and a Japanese oak branch extract.

Aging is a physiological phenomenon that cannot be avoided by anyone, and inevitable changes in the structure and function of the human body appear over time, which can be referred to as an aging phenomenon. Natural skin aging is a skin aging phenomenon observed in skin that has not been exposed to sunlight, and is also called intrinsic skin aging (chronological skin aging). Endogenous aging is caused by reactive oxygen species (ROS), which are generated during cellular metabolism in cells present in the skin, and extrinsic aging is caused by external factors such as ultraviolet (UV) and pollution.

The skin is largely composed of the epidermis, the dermis, and the subcutaneous fat layer. In particular, the dermis is a connective tissue between the epidermis and the subcutaneous fat layer, providing flexibility, elasticity and tension to the skin, and structurally supporting the epidermis. Fibroblasts in the dermal layer are responsible for the expression of collagen and elastin, which are fibrous proteins in the dermal layer. Synthesis of structural proteins such as the back decreases, moisture in skin cells is lost, and the structure of the stratum corneum is changed.

The biosynthesis of substrate materials is reduced by reduction of cellular activity and micro-inflammation due to naturally occurring endogenous aging, and by external factors such as an increase in stress caused by various harmful environments and an increase in reactive oxygen species caused by sunlight. Decomposition and degeneration accelerate, destroying the skin matrix and thinning, leading to various symptoms of skin aging. Therefore, many studies have been conducted on active ingredients that can prevent and improve such aging phenomena.

On the other hand, the North Korean name for yellow oyster mushroom is yellow mushroom, and it grows in groups on fallen trees or stumps of broad-leaved trees from summer to autumn. Fruiting bodies occur collectively in one tree, and the whole is 15cm in diameter and 10cm in height. Mushroom caps are 2~9cm in diameter, initially shaped like a bun, and later changed into a funnel shape. The surface of the lampshade is damp and flat, yellow or light yellow, with fluffy scales like white fibers attached to the center or edge. The flesh is white and smells of flour. Wrinkles are wrinkles that are first white and then turn yellow.

The oak tree is also called the bottom, and it is native to Asia, and grows in the sunny side of the foot of the mountain or on the bank of the field. The height reaches 3m and there are short hairs on the small branches, but they disappear soon. The bark is grayish brown. Leaves are alternate, 5-20cm long, egg-shaped or long egg-shaped, long and pointed at the end, and rounded at the bottom. The edge of the leaf has serrations, and it is deeply dug into 2-3 pieces. The front side is rough and there are short hairs on the back side, but they disappear soon. The petiole is 1~2cm long and there are curly hairs, but it gradually disappears. The flowers are male and female and bloom like leaves in spring. Male spikes are 1.5cm long oval-shaped and hang on the bottom of young branches. Each male flower has 4 pieces of pericarp and 4 stamens. The female ear is round and hangs on the leaf axil of the upper part of the branch. The female flower's inflorescence is tubular and the ends are split into 2-4 pieces. There is a style of style in the ovary. Fruit is drupe, round, and ripens red in June-July. In oriental medicine, the fruit is used as a cure for lack of yang and several species. Young leaves are edible.

As a result of researching to develop an anti-aging composition of a natural material with no toxicity and less side effects, the present inventors completed the present invention by confirming that the mixed extract of yellow oyster mushroom and black oak branch has an anti-aging effect.

In order to solve the above problems, the present invention aims to provide a composition for preventing or improving skin aging comprising a mixed extract of an extract of Pleurotus citrinopileatus and a branch extract of Broussonetia kazinoki as an active ingredient. do.

To achieve the above object,

The present invention provides a cosmetic composition for preventing or improving skin aging, comprising a mixed extract of yellow oyster mushroom ( Pleurotus citrinopileatus ) and a mixed extract of Broussonetia kazinoki as an active ingredient.

In addition, the present invention provides a health food composition for preventing or improving skin aging, comprising a mixed extract of a yellow oyster mushroom (Pleurotus citrinopileatus ) extract and a branch extract of Broussonetia kazinoki as an active ingredient.

The mixed extract of the yellow oyster mushroom extract and the oak branch extract of the present invention has excellent anti-aging effects, so it can be easily used in cosmetics, medicines, and the like.

1 is a diagram showing the results of cytotoxicity evaluation in skin fibrous cells (HDF) of a mixed extract of a yellow oyster mushroom extract and a Japanese oak branch extract.
Figure 2 is a diagram showing the results of cytotoxicity evaluation in skin fibrous cells (HDF) by mixing ratio of a mixed extract of a yellow oyster mushroom extract and a mulberry branch extract.
3 is a diagram showing the effect of inhibiting cell aging on skin fibrous cells (HDF) of a mixed extract of a yellow oyster mushroom extract and a mulberry branch extract.
4 is a diagram showing the effect of inhibiting cell aging on skin fibrous cells (HDF) by mixing ratio of a mixed extract of a yellow oyster mushroom extract and a Japanese oak branch extract.

Hereinafter, the present invention will be described in detail as an embodiment of the present invention with reference to the accompanying drawings. However, the following embodiments are presented as examples of the present invention, and if it is determined that a detailed description of a technique or configuration well known to those skilled in the art may unnecessarily obscure the subject matter of the present invention, the detailed description may be omitted. However, the present invention is not limited thereby. The present invention is capable of various modifications and applications within the scope of equality interpreted from the description of the claims to be described later and therefrom.

In addition, terms used in the present invention are terms used to properly express preferred embodiments of the present invention, which may vary depending on the intention of users or operators, or customs in the field to which the present invention belongs. Therefore, definitions of these terms should be made based on the contents throughout the present specification. Throughout the specification, when a part "includes" a certain component, it means that other components may be further included rather than excluding other components unless specifically stated to the contrary.

All technical terms used in the present invention, unless otherwise defined, are used in the same meaning as those of ordinary skill in the art generally understand in the related field of the present invention. In addition, although preferred methods or samples are described in the present specification, those similar or equivalent are included in the scope of the present invention.

The present invention provides a cosmetic composition for preventing or improving skin aging, comprising as an active ingredient a mixed extract of a yellow oyster mushroom ( Pleurotus citrinopileatus ) extract and a mulberry ( Broussonetia kazinoki) eggplant extract.

In one embodiment of the present invention, the mixed extract of the oyster mushroom extract and the black oak branch extract has an effect of inhibiting cell aging on skin fibrous cells.

The term “composition” used in the present invention refers to a mixture obtained by mixing other chemical components such as a diluent or carrier with the mixed extract of the yellow oyster mushroom extract and the Japanese oak branch extract of the present invention.

The term "vehicle" as used herein is defined as a compound that facilitates the addition of the compound into cells or tissues. For example, dimethyl sulfoxide (DMSO) is a commonly used carrier that facilitates the introduction of many organic compounds into cells or tissues of an organism, but is not limited thereto.

The term "diluent" as used herein is defined as a compound that is diluted in water to dissolve the compound as well as stabilize the biologically active form of the target compound. Salts dissolved in buffer solutions are used as diluents in the art. A commonly used buffer solution is phosphate buffered saline, because it mimics the salt state of human solutions. Because buffer salts can control the pH of a solution at low concentrations, buffer diluents rarely alter the biological activity of a compound.

The term "extract" used in the present invention refers to a formulation obtained by extracting a yellow oyster mushroom extract, a mulberry branch extract, and a mixed extract thereof with an appropriate extraction solvent and evaporating the solvent, but is not limited thereto, It may be an extract obtained by an extraction treatment, a dilution or concentrate of the extract, a dried product obtained by drying the extract, and a crude product or purified product thereof. The yellow oyster mushroom extract, Japanese oak branch extract, and a mixed extract thereof may be prepared using a general extraction method, separation and purification method known in the art. As the extraction method, although not limited thereto, a method such as hot water extraction, hot water extraction, cold needle extraction, reflux cooling extraction, or ultrasonic extraction may be used.

In one embodiment of the present invention, the mixture may be extracted after mixing yellow oyster mushrooms and mulberry branches, or the extracts may be mixed and used after extracting each of yellow oyster mushrooms and mulberry branches, and preferably, yellow oyster mushrooms and It may be a mixed extract obtained by mixing the extracts after extracting each of the mulberry branches.

In one embodiment of the present invention, the yellow oyster mushroom extract and the black oyster branch extract are extracted with a solvent selected from the group consisting of water, methanol, alcohol, organic solvents, and mixtures thereof, and preferably water or yellow oyster mushroom. It is not limited thereto if it is for the purpose of effectively extracting mushrooms and oak branches.

Each herbal or herbal extract may be used in a weight ratio of 1 to 7: 7 to 1, preferably using a weight ratio of yellow oyster mushroom extract and black oak branch extract in a weight ratio of 3: 5, but is not limited thereto. Does not.

The formulation of the cosmetic composition of the present invention is not particularly limited, but a formulation for external application for skin is preferred, and one preferred form of the formulation for external application for skin is a softening lotion, nutritional lotion, essence, massage cream, nutritional cream, pack, cleanser , A cosmetic composition having a formulation of a gel or a skin adhesive type cosmetic, and other preferred forms include a transdermal dosage form such as a lotion, ointment, gel, cream, patch or spray. In addition, in the composition for external application of each formulation, components other than the essential components described above may be appropriately selected and blended by a person skilled in the art without difficulty according to the formulation or purpose of use of other external preparations.

The cosmetic composition of the present invention preferably contains a cosmetically acceptable medium or base. These are all formulations suitable for topical application, e.g. solutions, gels, solid or paste anhydrous products, emulsions obtained by dispersing the oil phase in an aqueous phase, suspension microemulsions, microcapsules, microgranules or ionic (liposomes) and/or bis. It may be provided in the form of a warm-form vesicle dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. In addition, it may be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. In addition, the cosmetic composition of the present invention may be prepared in the form of an adjuvant that can be applied topically or systemically used in the field of dermatology by containing a dermatologically acceptable medium or base, and these compositions are conventional in the field. It can be manufactured according to the method.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component. I can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a solvating agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylenesorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

When the formulation of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linoline derivatives, or ethoxylated glycerol fatty acid esters may be used.

The formulation of the present invention may further contain excipients including fluorescent substances, fungicides, hydrophobic substances, moisturizers, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreen agents, vitamins, plant extracts, etc. .

In addition, the present invention provides a health food composition or a health functional food composition for preventing or improving skin aging, comprising a mixed extract of a yellow oyster mushroom (Pleurotus citrinopileatus ) extract and a branch extract of Broussonetia kazinoki as an active ingredient.

When using the mixed extract of the yellow oyster mushroom extract and Japanese oak branch extract of the present invention as a health functional food and a health food composition, there is no particular limitation on the type of food. Examples of foods to which the mixed extract of yellow oyster mushroom and mulberry branch of the present invention can be added include drinks, meat, sausage, bread, biscuits, rice cakes, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, ice cream Dairy products, including dairy products, various soups, beverages, alcoholic beverages and vitamin complexes, dairy products and dairy products, etc., and include all health functional foods and health food compositions in the usual sense.

The health functional food and health food composition containing the mixed extract of the yellow oyster mushroom extract and the mulberry branch extract according to the present invention can be added to food as it is or can be used with other foods or food ingredients, and can be used appropriately according to a conventional method. I can. The mixed amount of the mixed extract of the oyster mushroom extract and the blackberry branch extract may be appropriately determined according to the purpose of use (for prevention or improvement). In general, the amount of the composition in the health functional food and health food composition may be added in an amount of 0.1 to 90 parts by weight of the total food weight. However, in the case of long-term intake for the purpose of maintaining health or for the purpose of health control, the amount may be less than the above range, and there is no problem in terms of safety. It can also be used in more than one amount.

The health functional food and health food composition of the present invention is not particularly limited in other ingredients, except for containing a mixed extract of the present invention yellow oyster mushroom extract and blackberry branch extract as an essential ingredient in the indicated ratio, and various flavors such as ordinary beverages. Agents or natural carbohydrates, etc. may be contained as additional components. Examples of the above-described natural carbohydrates include monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, and the like; And polysaccharides, for example, common sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 of the health functional food and health food composition of the present invention.

In addition to the above, the health functional food and health food composition containing the mixed extract of the yellow oyster mushroom extract and the mulberry branch extract of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, Colorants and thickeners (cheese, chocolate, etc.), pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonates used in carbonated beverages, etc. It may contain. In addition, the health functional food and health food composition of the present invention may contain flesh for the manufacture of natural fruit juice and fruit juice beverage and vegetable beverage. These components may be used independently or in combination.

Hereinafter, the present invention will be described in more detail through the following examples. However, the following examples are only examples for easily explaining the content and scope of the technical idea of the present invention, and thereby the technical scope of the present invention is not limited or changed. In addition, based on these examples, it may be easily determined by those skilled in the art that various modifications and changes are possible within the scope of the technical idea of the present invention.

Experimental Example 1: Preparation of mixed extract of yellow oyster mushroom extract and Japanese oak branch extract

Yellow oyster mushroom ( Pleurotus citrinopileatus , cultivar long legs) was provided and used by the National Institute of Horticultural Science and Technology, Rural Development Administration. Specifically, yellow oyster mushrooms were freeze-dried, pulverized, and extracted by immersion in 10 times distilled water at room temperature for 24 hours. After extraction, it was filtered and lyophilized. 20 g of the dried yellow oyster mushroom was extracted to obtain 12.77 g of an extract.

The extract of the branch of Broussonetia kazinoki was provided by the National Institute of Horticultural Science and Technology, Ginseng Specialty Department, and was used by the Rural Development Administration. Specifically, the water extract of mulberry branch was obtained through the following manufacturing process. The branches of oak trees were collected from the hyperbolic in Mt. Jirisan and dried at 58°C for 3 days. 66.19 g of the dried oak branches were finely pulverized and extracted by immersing in 10 times distilled water at room temperature for 24 hours. The extract was filtered and lyophilized to obtain 5.51 g of the extract.

The obtained yellow oyster mushroom extract and blackberry branch extract were dissolved in distilled water at a concentration of 50 mg/ml to prepare a stock solution, and a mixed extract was prepared by mixing each extract stock solution.

Experimental Example 2: 　 Skin fibrous cell HDF cell line culture

Human dermal fibroblast (HDF) cells are prepared in a fibroblast growth medium containing antibiotics (penicillamine 100 unit/ml, streptomycin 100 g/ml) at 37°C, 5% CO ₂ Incubated under humidified air conditions. After that, when the cell density reached 80 ~ 90%, it was subcultured by trypsin treatment.

Experimental Example 3: Induction of cell senescence by treatment with doxorubicin

Skin fibrous cells were passaged in a cell culture dish with a diameter of 100 mm at a number of 1.5 x 10 ^{5 cells.} Incubated for 3 days in an incubator maintained at 37°C and 5% CO _2. The cell medium was removed, washed, and then treated with 0.5 μM doxorubicin for 4 hours to induce cell senescence. The skin fibrous cells induced cell aging with doxorubicin were cultured for 4 days with the extract of Experimental Example 1, and then cell senescence was measured.

Example 1: Cytotoxicity evaluation

The cytotoxicity of the mixed extract of oyster mushroom extract and Japanese oak branch extract was evaluated using the MTT assay.

Specifically, the skin fibrous cells inducing culture and cell aging according to Experimental Examples 2 and 3 were treated with the mixed extract of the oyster mushroom extract and the blackberry branch extract of Experimental Example 1 for 3 days. Thereafter, the skin fibrous cells were treated with a 0.1% MTT solution for 2 hours, and after removal of the medium, formazan crystals produced in the cells by MTT were dissolved in 100 μL of DMSO (dimethyl sulfoxide). Thereafter, the absorbance of the solution at 550 nm was measured using a microplate reader.

As a result, as shown in Figs. 1 and 2, cell viability in all of the group treated with yellow oyster mushroom alone, treated group with mulberry branches alone, and treated group with mixed extracts of yellow oyster mushroom extract and mulberry branch extract were all more than doxorubicin-treated group (Doxo). It was found to be high.

Therefore, it was confirmed that the extract of the present invention alone had no cytotoxicity.

Example 2: Measurement of cell aging inhibitory effect of mixed extract of oyster mushroom extract and Japanese oak branch extract

In order to measure the cell aging inhibitory effect of the mixed extract of yellow oyster mushroom extract and blackberry branch extract, a mixed extract of yellow oyster mushroom extract and blackberry branch extract was applied to skin fibrous cells inducing cell aging as in Experimental Example 3 for 3 days. After treatment, cell aging was measured by the SA-β-Gal method.

More specifically, the extract was treated by concentration and ratio to the skin fibrous cells treated with doxorubicin for 4 hours, and treated with 20 μM of N-acetylcysteine as a positive control, followed by treatment at 37° C., 5% for 3 days. Incubated in a carbon dioxide incubator. Thereafter, the medium was removed and the cells were fixed with 3.7% paraformaldehyde for 1 minute, and then the fixative was removed. After treatment with SA-β-gal staining solution (40 mM citric acid/phosphate [pH 5.8], 5 mM potassium ferrocyanide, 5 mM potassium ferricyanide, 150 mM NaCl, 2 mM MgCl ₂ , X-gal 1 mg/ml) Light-shielded with aluminum foil and reacted at 37°C for 18 hours. After washing twice with a phosphate buffer solution (PBS), cells stained in blue were observed with an optical microscope.

The cell aging index measured by SA-β-gal staining was expressed as a percentage (%) by measuring the number of cells stained blue in the cytoplasm among human fibroblasts.

As a result, as shown in Figure 3, when the mixing ratio of the mixed extract of the yellow oyster mushroom extract and the mulberry branch extract was 1:1, it was found that the cell aging inhibitory effect was excellent, and in particular, 200 μg/mL of the yellow oyster mushroom extract was treated alone. (Y200), compared with 200 μg/mL of black oak branch extract alone (P200), when treated with a mixture of 200 μg/mL of yellow oyster mushroom extract and 200 μg/mL of black oak branch extract (Y200 P200), the effect of inhibiting cell aging was increased. It turned out to be excellent.

Example 3: Measurement of cell aging inhibitory effect on skin cells by mixing ratio of mixed extract of yellow oyster mushroom and Japanese oak branch

In order to measure the cell aging inhibitory effect according to the mixing ratio of the mixed extract of the yellow oyster mushroom extract and the blackberry branch extract, the mixed extract of the yellow oyster mushroom extract and the blackberry branch extract was mixed with different mixing ratios to reduce cell aging as in Experimental Example 3 above. The induced skin fibrous cells were treated for 3 days and cell aging was measured by the SA-β-Gal method.

More specifically, the extract was treated by concentration and ratio on the skin fibrous cells treated with doxorubicin for 4 hours, and 20 μM of N-acetylcysteine was treated as a positive control. After that, it was incubated in an incubator at 37° C. and 5% carbon dioxide for 3 days. Thereafter, the medium was removed and the cells were fixed with 3.7% paraformaldehyde for 1 minute, and then the fixative was removed. After treatment with SA-β-gal staining solution (40 mM citric acid/phosphate [pH 5.8], 5 mM potassium ferrocyanide, 5 mM potassium ferricyanide, 150 mM NaCl, 2 mM MgCl ₂ , X-gal 1 mg/ml) Light-shielded with aluminum foil and reacted at 37°C for 18 hours. After washing twice with a phosphate buffer solution (PBS), cells stained in blue were observed with an optical microscope.

The cell aging index measured by SA-β-gal staining was expressed as a percentage (%) by measuring the number of cells stained blue in the cytoplasm among human fibroblasts.

As a result, as shown in Figure 4, when treated with a mixed extract (Y75 P125) of 75 μg/mL of yellow oyster mushroom extract and 125 μg/mL of blackberry branch extract (Y75 P125), the effect of inhibiting cell aging was excellent, and thus the mixture of yellow oyster mushroom and mulberry branch When the mixing ratio of the extract is 3: 5, it was found that the cell aging inhibitory effect was excellent, and in addition to the mixing ratio, the mixing ratio of yellow oyster mushroom extract and blackberry branch extract was 1:3, 3:5, 5:3, 3:1 and 7 : It was confirmed that the mixed extract of the yellow oyster mushroom extract and the Japanese oak branch extract of the present invention had superior cellular senescence inhibitory effect than each of the single extracts.

Claims (5)

A cosmetic composition for preventing or improving skin aging, comprising a mixed extract of yellow oyster mushroom ( Pleurotus citrinopileatus ) extract and oak tree (Broussonetia kazinoki) eggplant extract. The method of claim 1,
The extract is a cosmetic composition for preventing or improving skin aging, characterized in that the extract is extracted with any one solvent selected from the group consisting of water, C ₁ to C _{4 lower alcohol or a mixed solvent thereof.} The method of claim 1,
The composition is a cosmetic composition for preventing or improving skin aging, characterized in that it has a cell aging inhibitory effect. The method of claim 1,
A cosmetic composition for preventing or improving skin aging, characterized in that the mixing ratio of the yellow oyster mushroom extract and oak branch extract is any one of 1:3, 3:5, 5:3, 3:1, and 7:1. Yellow oyster mushroom ( Pleurotus citrinopileatus ) extract and black oak (Broussonetia kazinoki) a health food composition for preventing or improving skin aging, including a mixed extract of eggplant extract.

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