KR20210052218A - Probiotic composition containing monascus purpureus - Google Patents

Abstract

The present invention relates to a probiotic composition comprising Monascus purpureus. The probiotic composition comprising Monascus purpureus contains useful microorganisms to improve the weight gain rate and immunity of livestock and fish and increase feed efficiency, and can improve the productivity and meat quality of livestock and fish.

Description

Probiotic composition containing honggukgyun {PROBIOTIC COMPOSITION CONTAINING MONASCUS PURPUREUS}

The present invention relates to a probiotic composition comprising honggukgyun. More specifically, it relates to a probiotic composition containing honggukgyun which contains beneficial honggukgyun, can improve the gain rate and immunity of livestock and fish, improve feed efficiency, and improve the productivity and meat quality of livestock and fish. .

Feed additives are auxiliary substances that are mixed in small amounts in feed for the purpose of improving the productivity of livestock and fish. Antibiotics, probiotics, enzymes, organic acids, flavoring agents, sweeteners, antioxidants, various natural substances, and functional substances are classified as feed additives. Conventional feed additives have been manufactured to contain synthetic chemicals such as antibiotics or antibacterial agents for the purpose of improving the gain rate of livestock, increasing feed efficiency, and preventing diseases. It has been widely used as an antibiotic that helps livestock breeding by inhibiting the growth of harmful bacteria or microorganisms or destroying their lives.However, mixing and using these feed additives in livestock feed is a drug abuse for livestock, and this causes disease. Not only the immunity of domestic animals is lowered, but also the resistance to antibiotics is increased, resulting in the demand for stronger antibiotics. In addition, since drugs such as antibiotics remain in the body of livestock, the health of those who consume them may also be threatened. In fact, the EU banned the use of antibiotics as feed additives in 2006, and Korea also banned the use of antibiotics in blended feeds from 2011 for the same reason. Currently, the use of antibiotics for treatment is possible, but the use of antibiotics as feed additives is limited. Accordingly, research and development and efficacy studies of candidate materials with stability that can replace antibiotics and antibacterial agents harmless to livestock and humans for both consumers and producers in the animal industry are being conducted.

With the ban on the use of these antibiotics, probiotics are emerging. The present invention also relates to a probiotic, as a preparation containing beneficial living microorganisms, which settles in the intestine of animals to inhibit the growth of other pathogenic microorganisms, and serves to promote growth by helping digestion and absorption of feed consumed by livestock. It is preferable that the microorganism for probiotics possesses antibacterial activity against harmful bacteria such as E. coli and is capable of inducing a change in intestinal PH by promoting the production of lactic acid.

Feed probiotics can be broadly classified into bacterial, yeast, fungal and mixed types according to the type of microorganism. Bacterial probiotics include bacteria from the genus Lactobacillus, Streptococcus, Enterococcus, Bifidobacterium, Bacillus, and Bacteroides, and fungi from the genus Saccharomyces, which are fermented yeast, are mainly used for yeast probiotics. In addition to the above probiotic strains, it can be used as a strain of probiotics of various microorganisms, and various additions such as the production of antibacterial substances, production of enzymes, enhancement of immunity, synthesis of vitamins, decomposition of toxic substances, prevention of odor of livestock, etc. You can expect the effect.

Lactobacillus, which has been the most studied as a probiotic, produces digestive enzymes such as lactase, stimulates the production of lactic acid and volatile fatty acids, produces antimicrobial compounds such as hydrogen peroxide, and is beneficial in the intestine. It is known to increase the number of microorganisms and to reduce the concentration of serum cholesterol. However, although the Lactobacillus or lactic acid bacteria are good microorganisms, mass cultivation is difficult and cost is high, which is an economic problem. In particular, environmentally cultivation is difficult in Jeju Island or coastal areas with high humidity.

In general probiotic fermentation methods, there are differences in the culture system and culture conditions depending on the fermentation type, and the growth of microorganisms and products due to environmental factors such as moisture content, water activity, temperature, pH, oxygen level, and concentration of nutrients and products. It has a significant impact on its formation. Probiotic fermentation methods include Solid State Fermentations (SSF) and Liquid State Fermentations (LSF), and in general, the water content in the solid-state fermentation process is 30-75%, with a wide variation. Optimal moisture content maximizes the production of metabolites such as enzymes and organic acids. Water activity affects the development of biomass, metabolic reactions, and mass transfer processes, and its activity depends on the physical structure and chemical properties of the material. In addition, an increase in temperature during solid phase fermentation affects sporulation, growth, and metabolite formation. Solid-phase fermentation uses traditional fermentation starters such as Koji (Japan) and Ragi (Indonesia) to produce products with higher value, such as enzymes, organic acids, biopesticides, biofuels, and flavors, as well as bioremediation and biodegradation. It is used in various fields such as decomposition of harmful substances such as) and decomposition of agricultural waste.

Accordingly, there is a need for research and development and efficacy studies of microorganisms that are stable in place of antibiotics and antibacterial agents, and that are easy to cultivate in a humid environment such as Jeju Island or coastal areas with high humidity.

KR 10-2005-0099848 A KR 10-2019-0047756 A

It is an object of the present invention to provide a microorganism-based feed probiotic containing beneficial microorganisms.

Another object of the present invention is to provide a microbial-based feed probiotic that can improve the growth rate and immunity of livestock and fish by using beneficial microorganisms, thereby improving feed efficiency, and improving productivity and meat quality of livestock and fish. To provide.

In order to achieve the above object, the microbial-based feed probiotic according to an embodiment of the present invention is hongguk-gyun (Monascus purpureus); Photosynthetic bacteria; Gukbacteria; And yeast; includes.

The monascus purpureus is Monascus ruber, Monascus purpureus Went, Monascus rubervan Tieghem, Monascus puliginosus Sato (Monascus, fuliginosus) Monascus pilosus, Monascus pilosus Sato, Monascus anka, Monascus barykery, Monascus purpurens, Monascus purpurens It is a species selected from the group consisting of Sato (Monascus albidus Sato) and mixtures thereof.

The photosynthetic bacterium is Rhodobacter capsulatus.

The bacterium is selected from the group consisting of Hwangguk bacterium, Naphthosis bacterium, and Black Guk bacterium.

A feed composition according to another embodiment of the present invention is prepared using the microorganism-based feed probiotic.

The feed composition further includes rice husk, rice bran, tangerine juice, and tangerine meal.

The feed additive according to another embodiment of the present invention is prepared using the microorganism-based feed probiotic.

Hereinafter, the present invention will be described in more detail.

In the present specification, "feed efficiency" refers to an increase in weight compared to feed intake, and increasing feed efficiency refers to an increase in an increase in weight gain compared to feed intake, which can also be understood as improving digestibility or promoting growth. In addition, increasing feed efficiency can be understood in the same way as improving the feed demand rate ([feed intake/weight gain]×100, the amount of feed required to increase the weight of 1 kg of breeding animals), where the feed demand rate is the opposite of feed efficiency. By way of the calculation formula, "improving feed demand" can be understood as a decrease in the amount of feed required to increase the weight of 1 kg of the breeding animal. This improvement of feed efficiency or feed demand rate is directly linked to feed cost reduction.

In order to achieve the above object, the microbial-based feed probiotic according to an embodiment of the present invention is hongguk-gyun (Monascus purpureus); Photosynthetic bacteria; Gukbacteria; And yeast; includes.

Monascus purpureus is a microorganism of the genus Monascus, and has a characteristic of secreting a dark red pigment and a cholesterol biosynthesis inhibitor called monacolin K, and has been used for various purposes since hundreds of years ago. In food, hongguk is used as a food preservative and a coloring agent that gives red color, and the dyeing industry also uses red dye from hongguk. In addition to the food and dyeing industry, the most useful fields of honggukgyun are the secretion of a cholesterol biosynthesis inhibitor called monacholine K in hongguk, and are made by fermenting hongguk bacteria such as hongguk fermented liquor, hongguk fermented rice, and hongguk fermented bread. As food, it is used as a type of cholesterol inhibitor to lower blood cholesterol levels in humans.

Some of the fungi, including honggukgyun, have secondary metabolic processes in addition to the primary metabolic process. The primary metabolic process is a metabolic process that all living organisms have in common, and the secondary metabolic process cannot be observed in animals, and some microorganisms and plants. Although it is found in, it is not essential for survival, but through this secondary metabolic process, a wide variety of chemicals are produced. In the case of Hong Guk-gyun, cells grow and divide through the primary metabolic process, and through the secondary metabolic process, monacolin K, monascin, ankaflavin, rubropunctatin, and It secretes various kinds of chemicals, such as monascorubrin, rubropunctamine, and monascorubramine. Of these, Monacholine K inhibits the cholesterol biosynthesis process in animals. It works by lowering the cholesterol level in the animal's body.

It has long been known that Hong Guk-gyun is effective in lowering blood cholesterol levels in humans so that it was recorded for the purpose of blood circulation in the Chinese Ming Dynasty's classic book, Sijin Lee's. In addition, since the fermented food of Hong Guk-gyun has a blood sugar-enhancing effect and a blood pressure-lowering effect in addition to the purpose of lowering blood cholesterol, it is also used as a diabetes treatment adjuvant and a blood pressure-lowering adjuvant.

As described above, honggukgyun is widely used as a cholesterol-lowering drug and a cholesterol-lowering functional food, but it is rarely used for feed for livestock and fish until now.

In addition, photosynthetic bacteria are important microorganisms that form a group in the circulation circuit of plants in nature. They purify dirt such as human or animal excrement like heterotrophs, and crops or fish such as hydrogen sulfide or nitrogen compounds generated when organic matter is decomposed. And it proliferates by performing photosynthesis using carbon dioxide gas and solar energy while ingesting and removing substances harmful to livestock as their own food. It promotes the proliferation of beneficial microorganisms such as lactic acid bacteria and actinomycetes in the soil, increases the occurrence and proliferation of green microorganisms such as chlorella in water, and is used as food for zooplankton, the food chain of fish and shellfish. Therefore, photosynthetic bacteria exist anywhere where there is dirt, such as soil, rice paddies, lakes, coasts, and sewage treatment plants, and particularly, a large amount of photosynthetic bacteria exist in places where there is a lot of organic matter. In particular, photosynthetic bacteria absorb and grow harmful substances such as hydrogen sulfide (H ₂ S), nitrous acid, nitric acid, carbon dioxide (CO ₂ ), lower fatty acids, and organic acids as nutrients of the cells. In addition to its physiological function to purify the growing environment, it contains a large amount of protein, high-quality amino acids, nutrients such as vitamins, nucleic acids and red pigments, and physiologically active substances such as ubiquinone in the organism. It is being utilized.

In a broad sense, Aspergillus is a microorganism of the genus Aspergillus, which is an incomplete bacteria, and contains about 170 species. The genus Aspergillus shows similar properties to the genus Penicillium, but the genus Aspergillus likes the temperate zone, whereas the blue fungus likes the cold zone. Aspergillus bacteria have many important strains in the brewing industry or fermentation industry such as cheongju, soybean paste, and soy sauce. In a narrow sense, it refers to Aspergillus oryzae and A. sojae for brewing of cheongju, soybean paste, and soy sauce. These are known as yellow koji bacillus from the fact that any conidia are white, yellow, or yellow-green. In contrast to this, there are black koji bacteria whose conidia are black, and these include A. usanmii, A. saitoi, A. awamori, etc. These are strictly distinguished from black mold.

Many microorganisms in yeast have the ability to ferment sugars to produce ethanol and carbon dioxide, and are used in the manufacture of beer or fermentation of bread, and yeast itself is used in feed as an inexpensive source of fat and protein. It contains abundant vitamin B group, and some contain vitamin D. Recently, it is a strain that is also widely used in the pharmaceutical industry. Saccharomyces cerevisiae (Saccharomyces cerevisiae) contains digestive enzymes, has antimicrobial properties, and, in particular, has the advantage of promoting palatability and anti-pathy and activating beneficial bacteria in the intestine.

Microorganisms of the genus Lactobacillus are industrially used in the production of dairy products, beer, wine, and kimchi, and are also used as animal feed. Lactobacillus acidophilus is lactic acid production and weak heat resistance, antibiotics acidophilin, lactosine, ashdoline production, Lactobacillus plantarum is lactic acid production and strong bile resistance, antibiotic lactolin The production of Lactobacillus casei has properties of lactic acid production and gastric acid and enzyme resistance.

Specifically, in order to manufacture the microorganism-based feed probiotic, a culturing step of culturing the microorganism; A drying step of drying the microorganisms cultured through the culturing step; And a pulverizing step of pulverizing the microorganisms dried through the drying step, and may be obtained as a probiotic for feed.

More specifically, the culturing step of culturing the microorganism is liquid culture with a seed ampoule.

The step of pulverizing the microorganisms can generally be divided into coarse powder, medium powder, fine powder, and micro powder according to the degree of pulverization, and the size of the particles is The taste and flavor can be different, so it can be changed according to the purpose.

Preferably, the microbial powder of the present invention may be a medium powder of 2 to 4 mm.

Specifically, the microbial powder of the present invention is 6.35 to 12.7 mesh.

In the case of the particle size, it is possible to increase palatability by neutralizing the taste and flavor peculiar to the microbial powder, and to maintain the nutrient components inherent to the microbe well, so that it can be prepared as a feed probiotic with high palatability.

Preferably, the microbial-based feed probiotic may include 30 to 50 parts by weight of photosynthetic bacteria powder, 30 to 50 parts by weight of bacterium powder, and 30 to 50 parts by weight of yeast powder, based on 100 parts by weight of honggukgyun powder. When used as a mixed microorganism within the above range, the effect of promoting the growth of economically useful microorganisms can be properly exerted, and by improving the gain rate and immunity of livestock and fish, it is possible to increase feed efficiency, and the productivity of livestock and fish and It is possible to provide a composition that can exhibit the effect of improving meat quality.

The photosynthetic bacteria are not limited to a specific species, and are preferably R. sphaeroides, R. apigmentum, R. azotoformans, and Rhodobacter. Bacter blasticus, R. gluconicim, R. litoralis, R. massiliensis, R. veldkampii) and mixtures thereof, and preferably the photosynthetic bacterium is Rhodobacter capsulatus, but is not limited to the above example.

The Rhodobacter capsulatus is a photosynthetic bacterium and exhibits an effect of promoting the growth of useful microorganisms. In addition, it contains a carotenoid component with excellent immunity enhancing ability and bacteriochlorophyll a, which is important for body color production, so it has excellent immunity enhancing ability.

The bacterium is selected from the group consisting of Hwangguk bacterium, Naphthosis bacterium, and Black Guk bacterium.

Aspergillus oryzac is a species widely used for culturing seed cultures of soy sauce, soybean paste, and red pepper paste due to its excellent starch resolution and protein resolution. It is widely used in the production of enzyme proteins such as aspartic proteinase, an enzyme, and triglyceride lipase, which is a fatty acid degrading enzyme. Cultures obtained by cultivating these Bacillus are used as a microbial additive in poultry feed and are known to exert an effect on egg production and increase. In addition, the cultures of Bacillus bacteria are tryptophan, lysine, and methionine, which are easily insufficient in grain feeds such as corn. It contains a lot of vitamins B1, B2, pantothenic acid, biolin, folic acid, etc., such as threonine, etc.

Bacillus subtilis Natto is the most widely used microorganism in the production process of lead head, and it is Natto bacillus, a kind of Bacillus subtilis, which is a Bacillus subtilis, which is mainly boiled soybeans. It is similar to the manufacturing process of Cheonggukjang in that it is used and fermented in a warm place, but differs in that white spots and sticky mucus are formed on the surface of soybeans after fermentation and fermentation. It is harmless to the human body due to the crystal of tyrosine, a kind of amino acid, which is a seafood. Recently, Naphtha and Naphthabacteria, which have been considered only as traditional fermented foods, have begun to attract attention as feed additives such as poultry, and Hay bacillus is As a result of feeding to broilers, it is reported that it is effective for up to 5 weeks, but not after that, whereas Nattobacillus is isolated from lead heads, purely cultured, inoculated into grains (soybean meal), and dried. It has already been announced in the early 1990s that feeding with about 3% of it promotes growth and lowers feed demand.

When these are added to feed, the number of harmful microorganisms such as E. coli in the intestine is extremely reduced, and beneficial microorganisms such as lactic acid bacteria are proliferated, and the growth rate and feed rate can be improved.

A feed composition according to another embodiment of the present invention is prepared using the microorganism-based feed probiotic.

Consumers are increasingly aware of and sell quality meat and fish that are not only healthy but also taste good as their dietary life becomes more advanced and diversified. Therefore, in response to the demands of these consumers, producers are conducting many tests and studies to improve the quality of meat and fish products in various ways. In particular, research on blended feeds or feed additives is constantly being conducted in order to produce high-quality meat.

Accordingly, the present invention is made of a feed composition using a microbial-based feed probiotic, and has the advantage of improving the meat quality while increasing the growth rate of livestock and fish by beneficial microorganisms.

The livestock may be cattle, such as cattle, pigs, goats, sheep, horses, chickens, ducks, turkeys, and may be preferably pigs.

The fish may be aquatic products such as flatfish, yellowtail, mackerel, and dome, and preferably, flatfish.

The feed composition may include general basic feeds fed according to the types of livestock and fish, and these basic feeds are all known in the art for their necessary ingredients and compositions and suitable composition ratios according to the types of livestock and fish, In addition, since it is commercially available, anyone skilled in the art can easily manufacture or purchase it and use it.

The feed composition further includes rice husk, rice bran, tangerine juice, and tangerine meal.

Rice husk (hull, husk) refers to the outer skin of rice, and it is difficult to corrode because the inner and outer skin is densely covered with silicon, and is used as litter or compost for livestock, but recently, fermentation mixed with bio-microbial culture raw material and manure The use of compost is expanding. In general, the outer skin of grain contains antioxidants that can prevent oxidation and deterioration from the outside, and as antioxidants contained in rice husk, flavonoid, cyanidin, phytic acid, and ferulic acid are known. In particular, isovitexin, a C-glycosyl flavonoid, showed strong antioxidant ability in rice husks (Ramarathnam N, et al. 1989. J. Agric Food Chem 37:316-319), and 2.1 to 2.4% of phytic acid in rice husks was a metal chelate. It is said to have antioxidant activity by acting as a zero.

Rice bran is also called rice bran as the finest short bran produced when rice is pounded. Rice bran obtained in the process of milling rice contains more than 40 kinds of proteins, lipids, B vitamins, minerals, fiber, and many very useful trace elements. Recently, various functionalities of rice bran have been studied, and in particular, there are many reports on the anti-stress effect, anti-fatigue effect, and immunity enhancing effect of rice bran.

Fruit meals produced after beverage processing of fruits contain various types of physiologically active substances. In particular, in the case of citrus peels, it takes a lot of cost and effort to exhaust the juice by-products during the harvest season. It is true that the method of utilizing the discarded functional substances of citrus gourd in livestock and the functional substances are not being properly utilized. In particular, hesperdin, a polyphenol component rich in citrus, is a physiologically active substance with antioxidant effects, and by ingesting it by livestock, it can promote health by promoting metabolism and strengthening immunity.

Accordingly, the present invention includes a citrus juice with excellent antioxidant power, and can be safely fed to livestock and provides a feed composition as an immune enhancing agent or an immune function modulator.

Preferably, the feed composition of the present invention further comprises stone agar powder and lump leaf powder in addition to the rice hull, rice bran, tangerine juice and tangerine meal.

Chondracanthus tenellus (Harvey) Hommersand in Hommersand) is a red algae plant belonging to the family Stony agaraceae, and lives on rocks 2 to 4 m deep in the sea, and its height is 5 to 12 cm. Cylindrical branches are misaligned or faced several times, and the ends of the branches are pointed. The color is purple or reddish-purple, and the round pockets are small and there is no bag. Those that grow in shallow places are small, and those that grow in deep places are large. It is distributed in Japan, and in Korea, it is distributed all over the country.

Lithophyllum okamurai is a red algae plant, about 0.6mm thick, covers the surface of small stones or other objects, and grows with lump-shaped projections. The whole body is covered with lime and has a pink or red color. The circumferential projection corresponds to the branch, and the end is round and the surface is smooth. In Korea, it is collected from Jeju and the south coast.

Preferably, the feed composition includes 10 to 20 parts by weight of rice husk, 10 to 20 parts by weight of rice bran, 10 to 20 parts by weight of tangerine juice, and 5 to 10 parts by weight of tangerine juice, based on 100 parts by weight of the microorganism-based feed probiotic of the present invention. I can.

More preferably, the feed composition is based on 100 parts by weight of the microbial-based feed probiotic of the present invention, rice husk 10 to 20 parts by weight, rice bran 10 to 20 parts by weight, orange juice 10 to 20 parts by weight, orange peel 10 to 20 parts by weight, It may include 5 to 10 parts by weight of stone agar powder and 5 to 10 parts by weight of black stone leaf powder.

When used as a mixed composition within the above range, the effect of promoting the growth of economically useful microorganisms can be properly exerted, and can be provided as a feed composition that can further enhance the efficacy of improving meat quality and growth ability of livestock and fish. have.

The feed additive according to another embodiment of the present invention is prepared using the microorganism-based feed probiotic.

Preferably, the feed composition for pigs according to another embodiment of the present invention may include the microorganism-based probiotic.

Preferably, the feed composition for flatfish according to another embodiment of the present invention may include the microorganism-based probiotic.

According to the microorganism-based feed probiotic of the present invention, it contains beneficial microorganisms, improves the gain rate and immunity of livestock and fish, can increase feed efficiency, and can exhibit the effect of improving productivity and meat quality of livestock and fish. Probiotics for feed of can be provided.

1 shows the culture of honggukgyun according to an embodiment of the present invention.
2 is a graph confirming the survival rate of a microorganism-based feed probiotic according to an embodiment of the present invention for 4 weeks at room temperature.
3 is a graph confirming a change in pH of a microbial-based feed probiotic according to an embodiment of the present invention for 4 weeks at room temperature.
Figure 4 is a graph showing the effect of erythema honggukgyun according to an embodiment of the present invention.
5 is a photograph of a flounder confirming the effect of honggukgyun according to an embodiment of the present invention.
6 shows the result of measuring the protein expression pattern expressed in pig loin according to an embodiment of the present invention by 1-electrophoresis and silver staining.

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily implement the present invention. However, the present invention may be implemented in various different forms and is not limited to the embodiments described herein.

[Production Example 1: Preparation of beneficial microorganisms]

One. Preparation of honggukgyun powder

culture

In order to supply sufficient oxygen, the spawn Monascus purpureus (ATCC 34892) purchased from the Center for Biological Resources, 40 ml of YEME (3g yeast extract, 5g peptone, 3g malt extract, 340g sucrose, 10g glucose, 1g MgCl ₂ ·6H) per 250 ml of flask ₂ O) 0.2% (200 µl seed per 100 ㎖ growth medium) was inoculated into a baffled flask containing the medium, followed by liquid culture for 2 to 4 days.

After that, using a Monascus medium and a PDA agar medium were cultured in the form of cubes as shown in FIG. 1 for 5 to 8 days.

Preparation of honggukgyun powder

The cultured honggukgyun was naturally dried for a long time at room temperature of 18°C to 20°C, and then pulverized into a powder of 10 mesh or more in a completely dried state to prepare honggukgyun powder (MG).

2. Preparation of other microbial powder

Yeast was used as Saccharomyces cerevisiae, and photosynthetic bacteria Rhodobacter capsulatus and Hwangguk bacteria were used in the same manner as in the preparation of the honggukgyun powder, and photosynthetic bacterial powder (RG), Hwangguk bacteria powder (AG) and yeast powder (YG) were mixed as shown in Table 1 below to prepare a microbial-based feed probiotic.

BF1 BF2 BF3 BF4 BF5 BF6 MG 100 100 100 100 100 100 RG - 20 30 40 50 60 AG - 20 30 40 50 60 YG - 20 30 40 50 60

(Unit: parts by weight)

[Experimental Example 1: Viability and pH confirmation of microbial-based feed probiotics]

Cytotoxicity

In order to test the toxicity of the microbial-based feed probiotics BF1 to BF6 prepared in Preparation Example 1, the difference in toxicity and side effect expressions were confirmed when the probiotic was administered in a rat repeated dose toxicity test.

BF1 to BF6 were administered by dividing 6-week-old male and female rats with SD patency into 10 rats per group (5 males and females each).After dissolving each probiotic in 0.5% MC solution, oral administration once daily at the same morning time was 13 Repeated for weeks.

One dose was administered in an amount of 3.75 mg/kg to 5 mg/kg. Afterwards, mortality, general symptoms, weight change, and feed and water intake were observed.

As a result, no dead individuals occurred within the experimental period. In view of the above experimental results, it was confirmed that the microbial-based feed probiotics BF1 to BF6 prepared in Preparation Example 1 were not problematic in toxicity.

Viability and pH check

While storing the microbial-based feed probiotics BF1 to BF6 at room temperature for 4 weeks, the survival rate of the probiotics according to each range was confirmed by the CFU counting method, and each pH is a pH meter (pH/ISE Meter 735P, Istek Co., Ltd, Seoul, Korea).

The results are shown in FIGS. 2 and 3 below, and it was confirmed that in the case of probiotics in the range of BF3 to BF5, the viability was maintained remarkably high (FIG. 2).

In addition, the probiotics according to the range of BF1 and BF2 were found to be pH 6.5 and pH 6.0, and the probiotics according to the BF6 range showed a higher pH than 9.0, whereas the pH of the probiotics according to the range of BF3 to BF5 It was confirmed that it appeared stably without almost any change (Fig. 3). In particular, in the case of BF4, it showed the most stable pH at pH 7.8.

[Production Example 2: Preparation of feed composition]

Among the microbial-based feed probiotics prepared in Preparation Example 1, rice hull, rice bran, tangerine juice, tangerine meal, stone agar powder and black stone leaf powder were included in the probiotics according to the range of BF4 having high viability and the most stable pH. A feed composition was prepared in the same composition as in 2.

MP1 MP2 MP3 MP4 MP5 BF4 100 100 100 100 100 chaff - 5 10 20 30 Rice bran - 5 10 20 30 Tangerine juice - 5 10 20 30 Tangerine - 5 10 20 30 Stone agar powder - - 5 10 20 Black Stone Leaf Powder - - 5 10 20

(Unit: parts by weight)

[Experimental Example 2: Effect of improving feed efficiency]

One. Ethosis due to the addition of hongguk-gyun

In order to check whether or not the honggukgyun powder MG (BF1) prepared using the honggukgyun of the present invention was used, flounder (flounder) weighing 5kg was stocked in a water tank, and the experimental group administered MG (BF1) honggukgyun powder was not added. Divided into a non-control group and grown for 10 days.

The results are shown in Table 3, FIGS. 4 and 5 below.

division Control Experimental group Starting average weight 5 6 Average weight at the end of the experiment 13.5 15.7 Average weight gain 9 10 Average daily weight gain per head 0.9(100) 1.67(186)

(Unit: Kg)

As shown in Table 3, it was confirmed that the daily weight gain was increased by 186% compared to the experimental group 1.67kg to which the honggukgyun powder was administered to 0.9kg for the control group without addition.

2. Increase in weight gain

Feed compositions MP1 to MP5 prepared in Preparation Example 2 were mixed with basic feed as shown in Table 4 below and fed to pigs. Basic feed is a feed that contains corn, wheat, wheat bark, soft seed, and rapeseed as the main raw materials.

division Salary feed Control Basic feed Experimental group 1 Basic feed + feed composition of the present invention MP1 Experimental group 2 Basic feed + feed composition of the present invention MP2 Experimental group 3 Basic feed + feed composition of the present invention MP3 Experiment group 4 Basic feed + feed composition of the present invention MP4 Experimental group 5 Basic feed + feed composition of the present invention MP5

The feed was freely vegetarian, and water was freely drinkable using an automatic water supply. Feeding management was carried out in accordance with the general feeding management method, and the pig area and water supply facilities were kept under the same conditions between the control group and the experimental group. The experiment period was 5 weeks, and the daily weight gain, daily feed intake, and feed efficiency (weight gain/feed intake) were calculated based on the start of the experiment and the end of the experiment. The results are shown in Table 5 below.

division Daily weight gain (Kg) Daily feed intake (g) Feed efficiency Control 0.408±0.047 0.743±0.062 0.549±0.026 Experimental group 1 0.462±0.055 0.735±0.042 0.628±0.034 Experimental group 2 0.469±0.052 0.742±0.048 0.632±0.040 Experimental group 3 0.482±0.033 0.748±0.042 0.644±0.037 Experiment group 4 0.501±0.018 0.752±0.070 0.666±0.044 Experimental group 5 0.472±0.043 0.738±0.049 0.639±0.046

The results of Table 5 show that feed efficiency increases by approximately 8 to 9% when feed compositions MP1 to MP5 are added to the basic feed. In addition, in the case of Experimental Group 1, Experimental Group 2, and Experimental Group 5, which mixed MP1, MP2 and MP5 with basic feed, the feed efficiency tended to slightly increase, but there was no significant difference in the difference. It was confirmed that the feed efficiency tends to increase significantly in the case of 3 and experimental group 4.

3. Antioxidative effect

In order to compare the effect on the immunity according to the feed composition MP1 to MP5 feed of the present invention, the antioxidant effect was measured. The homeostasis effect was measured by an electron donating ability (DPPH, (1,1-diphenyl-2-picrylhydrazyl)) assay.

The results are shown in Table 6 below.

division DPPH(%) Antioxidant activity increase rate (%) Control 120 Experimental group 1 437 Experimental group 2 559 Experimental group 3 832 Experiment group 4 995 Experimental group 5 499

Compared to the feed of the control group, the antioxidant activity was also increased in the feeds of Experimental Group 1, Experimental Group 2, and Experimental Group 5 with the feed composition of MP1, MP2, and MP5 added. In case 4, it was confirmed that the antioxidant activity was significantly increased.

[Experimental Example 3: Meat Quality Improvement Effect]

First, 6 heads were selected from pork loin with a good meat quality to pork loin with a low grade, and the expression patterns of proteins expressed from pig muscle tissue (loin) for each grade were confirmed. Two types of electrophoresis were performed according to acrylamide% using 1D-electrophoresis and silver staining, and then protein expression patterns were measured, and the results are shown in FIG. 6.

As shown in Figure 6, the protein expression pattern in the pig muscle tissue (loin) according to the grade is HQLD (high quality of longissimus dorsi (1-6)) for pork loin with good meat quality, LQLD for pork loin with low meat quality ( low quality of longissimus dorsi (7~12)), it was confirmed that proteins according to grades were differentially expressed at 130kDa(a*) and 34kDa(b#), respectively.

Therefore, based on this, the proteins expressed in the pork loin of Experimental Groups 1 to 5 according to Example 2 were measured for protein expression in the same manner as described above. Comparative analysis of the protein HQLD (high quality of longissimus dorsi (1-6)) of pork loin with good meat quality, and the protein LQLD (low quality of longissimus dorsi (7-12)) of pork loin with low meat quality in Table 7 below. And, for relative comparison, pigs having good meat quality, expressing the protein of HQLD, were set as index 5, and the protein expression patterns of experimental groups 1 to 5 were evaluated as indexes 1 to 10.

It would be said that the higher the index, the better the protein expression pattern.

Pig with good meat quality Experimental group 1 Experimental group 2 Experimental group 3 Experiment group 4 Experimental group 5 HQLD 5 6 7 8 8 7 LQLD 0 2 One 0 2 2

(Unit: index)

As shown in Table 7 above, compared with the protein expression patterns of pigs having good meat quality, it was confirmed that proteins of equal or higher levels were expressed in Experimental Groups 1 to 5.

In particular, it was confirmed that the protein expression pattern was highly evaluated in Experimental Group 3 and Experimental Group 4 in which MP3 and MP4 were mixed with basic feed, and improved meat quality.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention defined in the following claims are also present It belongs to the scope of rights of

Claims (3)

Monascus purpureus powder; 30 to 50 parts by weight of photosynthetic bacteria powder based on 100 parts by weight of the red guk bacterium powder; Mixed microorganisms comprising 30 to 50 parts by weight of Hwangguk bacteria powder and 30 to 50 parts by weight of yeast powder,
Based on 100 parts by weight of the mixed microorganism, 10 to 20 parts by weight of rice husk; 10 to 20 parts by weight of rice bran; 10 to 20 parts by weight of tangerine juice; 10 to 20 parts by weight of orange peel; Including 5 to 10 parts by weight of stone agar powder and 5 to 10 parts by weight of black stone leaf powder,
The yeast is Saccharomyces cerevisiae.
Microbial-based feed probiotic. Containing a microbial-based feed probiotic according to claim 1
Feed composition Containing a microbial-based feed probiotic according to claim 1
Feed additives.

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