KR102407131B1 - Method for preparing a probiotic composition for feed to improve body weight gain or immunity - Google Patents

Abstract

The present invention relates to a method for producing a probiotic composition for feed that improves the growth rate or immunity. have. Therefore, according to the present invention, it is possible to provide a probiotic composition comprising Hong Guk-gyun, which can improve the productivity and meat quality of livestock and fish.

Description

Method for preparing a probiotic composition for feed to improve body weight gain or immunity {Method for preparing a probiotic composition for feed to improve body weight gain or immunity}

The present invention relates to a method for producing a probiotic composition for feed that improves the growth rate or immunity. More specifically, a method for preparing a probiotic composition for feed, which contains beneficial Hongguk bacterium, improves the growth rate and immunity of livestock and fish, can increase feed efficiency, and can exhibit the effect of improving the productivity and meat quality of livestock and fish is to provide

Feed additives are auxiliary substances that are mixed in small amounts in feed for the purpose of improving productivity of livestock and fish. Antibiotics, probiotics, enzymes, organic acids, flavoring agents, sweeteners, antioxidants, various natural substances, functional substances, etc. are classified as feed additives. Conventional feed additives have been manufactured to contain antibiotics or antibacterial agents, which are synthetic chemicals, for the purpose of improving the growth rate of livestock, increasing feed efficiency, and preventing diseases. It has been widely used as an antibiotic to help breed livestock by inhibiting the growth of harmful bacteria or microorganisms or destroying their life. As the immunity of the livestock to antibiotics decreases as well as the resistance to antibiotics increases, it results in a demand for stronger antibiotics. In addition, since drugs such as antibiotics remain in the body of livestock, the health of people who consume them may be threatened. In fact, the EU banned the use of antibiotics as feed additives in 2006, and for the same reason, Korea banned the use of antibiotics in compounded feeds from 2011. Currently, the use of antibiotics for treatment is possible, but the use of antibiotics as feed additives is limited. Therefore, in the animal industry, for both consumers and producers, research and development and efficacy studies of candidate substances with safety that can replace antibiotics and antibacterial agents harmless to livestock and human body are being conducted.

As the use of these antibiotics has been banned, probiotics have emerged. The present invention also relates to a probiotic, which is a preparation containing live beneficial microorganisms, which settles in the intestine of an animal to inhibit the growth of other pathogenic microorganisms, and promotes growth by helping the digestion and absorption of feed consumed by livestock. It is preferable that the microorganisms for probiotics have antibacterial activity against harmful bacteria such as E. coli and can induce changes in intestinal PH by promoting lactic acid production.

Probiotics for feed can be broadly classified into bacterial, yeast, fungal and mixed types according to the type of microorganisms. Bacterial probiotics include bacteria of the genera Lactobacillus, Streptococcus, Enterococcus, Bifidobacterium, Bacillus, and Bacteroides, and the yeast probiotic is mainly used as a fermenting yeast, Saccharomyces. In addition to the above probiotic strain, it can be used as a probiotic strain of various microorganisms, and various additions such as production of antibacterial substances, production of enzymes, enhancement of immunity, synthesis of vitamins, decomposition of toxic substances, prevention of odor of livestock, etc. for each characteristic of the strain effect can be expected.

Lactobacillus, which has been studied the most as a probiotic, produces digestive enzymes such as lactase, stimulates the production of lactic acid and volatile fatty acids, produces antimicrobial compounds such as hydrogen peroxide, and beneficial intestinal It is known to increase the number of microorganisms and to reduce the concentration of serum cholesterol. However, although the Lactobacillus or lactic acid bacteria are good microorganisms, the fact that mass culture is difficult and expensive is a problem in terms of economical efficiency. In particular, there are environmental difficulties in culturing in Jeju Island or coastal areas with high humidity.

In the general probiotic fermentation method, there are also differences in culture system and culture conditions depending on the fermentation type, and microbial growth and product have a significant impact on the formation There are two types of fermentation methods for probiotics: Solid State Fermentations (SSF) and Liquid State Fermentations; LSF. The optimum water content maximizes the production of metabolites such as enzymes and organic acids. Water activity affects the development of biomass, metabolic reactions, and mass transfer processes, and its activity depends on the physical structure and chemical properties of the substance. In addition, an increase in temperature during solid-state fermentation affects sporulation, growth, and formation of metabolites. Solid fermentation uses traditional fermentation starters such as Koji (Japan) and Ragi (Indonesia) to produce products with increased values such as enzymes, organic acids, biological pesticides, biofuels and flavors, as well as bioremediation and biodegradation. ) is used in various fields such as decomposition of harmful substances such as decomposition of agricultural waste.

Therefore, there is a need for research and development and efficacy studies of microorganisms that are stable as a substitute for antibiotics and antibacterial agents and that are easy to culture even in humid environments such as Jeju Island or coastal areas with high humidity.

KR 10-2005-0099848 A KR 10-2019-0047756 A

It is an object of the present invention to provide a method for producing a microorganism-based probiotic for feed containing beneficial microorganisms.

Another object of the present invention is to use beneficial microorganisms to improve the growth rate and immunity of livestock and fish, thereby increasing feed efficiency, and improving the productivity and meat quality of livestock and fish. To provide a manufacturing method for

In order to achieve the above object, the microorganism-based feed probiotic according to an embodiment of the present invention is Hong Guk-gyun (Monascus purpureus); photosynthetic bacteria; Kukkyun; and yeast;

The Hong Guk-gyun (Monascus purpureus) is Monascus ruber (Monascus ruber), Monascus Purpureus Went (Monascus purpureus Went), Monascus rubervan Tieghem (Monascus rubervan Tieghem), Monascus fuliginosus Sato (Monascus fuliginosus Sato), Monascus pilosus, Monascus pilosus Sato, Monascus anka, Monascus barykery, Monascus purpurens albidu It is a species selected from the group consisting of Sato (Monascus albidus Sato) and mixtures thereof.

The photosynthetic bacteria is Rhodobacter capsulatus.

The Kukkyun is selected from the group consisting of Hwanggukgyun, Napdugyun and Heukgukgyun.

The feed composition according to another embodiment of the present invention is prepared by using the microorganism-based probiotic for feed.

The feed composition further comprises rice husk, rice bran, tangerine juice and tangerine gourd.

The feed additive according to another embodiment of the present invention is prepared by using the microorganism-based probiotic for feed.

Hereinafter, the present invention will be described in more detail.

As used herein, "feed efficiency" refers to the amount of gain compared to the feed intake, and increasing the feed efficiency means that the increase in the amount of gain compared to the feed intake is improved, which can also be understood as the meaning of improving digestibility or promoting growth. In addition, increasing feed efficiency can be understood in the same way as improving the feed demand rate ([feed intake/gain] × 100, the amount of feed required to increase the body weight of a breeding animal by 1 kg), where the feed demand rate is opposite to the feed efficiency. According to the calculation formula, "improving the feed demand rate" can be understood as a decrease in the amount of feed required to increase the body weight of the breeding animal by 1 kg. Such improvement of feed efficiency or improvement of feed demand rate is directly related to feed cost reduction.

In order to achieve the above object, the microorganism-based feed probiotic according to an embodiment of the present invention is Hong Guk-gyun (Monascus purpureus); photosynthetic bacteria; Kukkyun; and yeast;

Hong Guk-gyun (Monascus purpureus) is a microorganism of the genus Monascus and has a characteristic of secreting a dark red pigment and a cholesterol biosynthesis inhibitor called monacolin K, and has been used for various purposes since hundreds of years ago. In food, red yeast rice is used as a food preservative and a red colorant, and in the dyeing industry, the red dye of red yeast is also used. In addition to the food and dyeing industries, the fields in which red yeast bacteria are most usefully utilized for mankind are various fields made by fermenting red yeast rice, such as red yeast rice wine, fermented red yeast rice, and fermented red yeast bread, using the property of secreting the cholesterol biosynthesis inhibitor called monacolin K of red yeast rice. As food, it is used as a kind of cholesterol inhibitor that lowers blood cholesterol levels in humans.

Some fungi, including Hong Guk-gyun, have secondary metabolic processes in addition to the primary metabolic process. The primary metabolic process is a common metabolic process for all living things to survive. Although not essential for survival, a wide variety of chemical substances are produced through this secondary metabolic process. In the case of Hong Guk-gyun, cells grow and divide through the primary metabolic process, and through the secondary metabolic process, monacolin K, monascin, ankaflavin, rubropunctatin, It secretes various types of chemicals such as monascorubrin, rubropunctamine, and monascorubramine. It works by lowering cholesterol levels in the animal's body.

It has been known for a long time that Hong Guk-gyun is effective in lowering blood cholesterol levels in humans to the extent that it was recorded for the purpose of blood circulation in Lee Si-jin's herbal medicine, a classic Chinese medical book. In addition, the fermented food of Hong Guk-gyun has blood sugar strengthening and blood pressure lowering actions in addition to the purpose of lowering blood cholesterol.

As such, Hong Guk-gyun is widely used as a cholesterol-lowering drug and cholesterol-lowering functional food, but it is hardly used for feed of livestock and fish until now.

In addition, photosynthetic bacteria are important microorganisms that form a group in the plant cycle in the natural world. They purify wastes such as human and animal excrement like heterotrophs, and crops and fish such as hydrogen emulsification or nitrogen compounds that are produced when organic matter is decomposed. And while ingesting and removing substances harmful to livestock as their food, they multiply by photosynthesis using carbon dioxide and solar energy. In soil, it promotes the growth of beneficial microorganisms such as lactic acid bacteria and actinomycetes, and in water increases the occurrence and proliferation of green microorganisms such as chlorella, and is used as food for zooplankton, the food chain of fish and shellfish. Therefore, photosynthetic bacteria are present wherever there is filth, such as soil, rice paddies, lakes, coasts, and sewage treatment plants, and in particular, exist in large amounts in places where there is a lot of organic matter. In particular, photosynthetic bacteria absorb hydrogen sulfide (H ₂ S), nitrous acid, nitric acid, carbon dioxide (CO ₂ ), lower fatty acids, organic acids, etc., which are harmful substances generated in the growing environment of crops, livestock, and fish, as nutrients and multiply by absorbing them. In addition to the physiological function of purifying the growth environment, it contains a large amount of protein, high-quality amino acids, vitamins, nucleic acids, nutrients such as red pigment, and physiologically active substances such as ubiquinone in the body of the bacteria. is being utilized

In a broad sense, Aspergillus is a microorganism of the genus Aspergillus, which is an imperfect bacterium, and contains about 170 species. The genus Aspergillus has properties similar to those of the genus Penicillium, but the genus Aspergillus prefers a temperate zone, whereas the genus Aspergillus prefers a low temperature zone. There are many important strains in the brewing industry or fermentation industry, such as sake, soybean paste, and soy sauce in the Aspergillus genus. In a narrower sense, it refers to Aspergillus oryzae and A. sojae for brewing sake, soybean paste, and soy sauce. All of these are called yeast yeast from the ones whose conidia are white, yellow, or yellow-green. In contrast to this, there are black yeast bacteria with black conidia, which include A. usanmii, A. saitoi, A. awamori, and the like. These are strictly distinct from black mold.

Many microorganisms in the genus yeast have the ability to produce ethanol and carbon dioxide by fermenting sugar, so they are used in the manufacture of beer or the fermentation of bread, and yeast itself is used in feed as a cheap fat and protein source. They are rich in B vitamins, and some contain vitamin D. Recently, it is a strain that is also widely used in the pharmaceutical industry. Saccharomyces cerevisiae (Saccharomyces cerevisiae) contains digestive enzymes, has antibacterial properties, and in particular, has the advantage of enhancing palatability and anti-military force and activating beneficial bacteria in the intestine.

The microorganisms of the genus Lactobacillus are industrially used in the production of dairy products, beer, wine, kimchi, etc., and are also used as animal feed. Lactobacillus acidophilus produces lactic acid and weak heat resistance, antibiotics acidophylline, lactosin, and acidoline are produced, Lactobacillus plantarum produces lactic acid and strong bile resistance, antibiotic lactolin Production of, Lactobacillus casei has the characteristics of lactic acid production and gastric acid and enzyme resistance.

Specifically, in order to produce the microorganism-based probiotic for feed, a culturing step of culturing the microorganism; a drying step of drying the microorganisms cultured through the culturing step; And it can be obtained as a probiotic for feed, including a grinding step of pulverizing the microorganisms dried through the drying step.

More specifically, the culturing step of culturing the microorganism is a liquid culture with a seed ampule.

The step of pulverizing the microorganism can be generally divided into coarse powder, medium powder, fine powder, and micro powder depending on the degree of pulverization, and the size of the particles is The taste and flavor can be different, so it can be changed according to the use.

Preferably, the microbial powder of the present invention may be a medium powder of 2 to 4 mm.

Specifically, the microbial powder of the present invention is 6.35 to 12.7 mesh (Mesh).

In the case of the particle size, it is possible to increase palatability by neutralizing the unique taste and flavor of the microbial powder, and it is possible to maintain the nutrients inherent in microorganisms well, so that it can be prepared as a probiotic for feed with high palatability.

Preferably, the microorganism-based probiotic for feed may include 30 to 50 parts by weight of photosynthetic bacteria powder, 30 to 50 parts by weight of yeast powder, and 30 to 50 parts by weight of yeast powder with respect to 100 parts by weight of honggukgyun powder. When used as a mixed microorganism within the above range, the effect of economically promoting the growth of useful microorganisms can be properly exhibited, and by improving the growth rate and immunity of livestock and fish, feed efficiency can be increased, and the productivity and It is possible to provide a composition capable of exhibiting an effect of improving meat quality.

The photosynthetic bacteria are not limited to a specific species, and preferably, R. sphaeroides, R. apigmentum, R. azotoformans, R. azotoformans, Rhodobacter. Bacter blasticus (R. blasticus), Rhodobacter gluconicum (R. gluconicim), Rhodobacter litoralis (R. litoralis), Rhodobacter masiliensis (R. massiliensis), Rhodobacter belt campii (R) veldkampii) and mixtures thereof, preferably the photosynthetic bacterium is Rhodobacter capsulatus, but is not limited thereto.

The Rhodobacter capsulatus is a photosynthetic bacterium, and exerts an effect of promoting the growth of useful microorganisms. In addition, it contains a carotenoid component with excellent immunity strengthening ability and bacteriochlorophyll a, which is important for body color production, so it has excellent immunity strengthening ability.

The Kukkyun is selected from the group consisting of Hwanggukgyun, Napdugyun and Heukgukgyun.

Aspergillus oryzac is a species widely used for culturing the seed culture of soy sauce, soybean paste, and red pepper paste because of its excellent starch and proteolytic properties. It is widely used in the production of enzyme proteins such as aspartic proteinase, an enzyme, and triglyceride lipase, a fatty acid degrading enzyme. The cultured culture of these koji bacteria is used as a microbial feed additive in poultry feed and is known to exert an effect on egg-laying rate and growth growth. Also, the cultured culture of koukbacterium is tryptophan, lysine, and methionine, amino acids that are easily lacking in grain feeds such as corn. , threonine, and vitamins B1, B2, pantothenic acid, biorin, and folic acid.

Bacillus subtilis Natto is the most widely used microorganism in the manufacturing process of natto, and it is Natto bacillus, a type of Bacillus subtilis. Natto is mainly made from boiled soybeans. It is similar to the manufacturing process of cheonggukjang in that it is used and fermented in a warm place, but is different in that white spots and sticky mucus are formed on the surface of soybeans after fermentation and fermentation. It is harmless to the human body due to the crystals of Tyrosine, a kind of amino acid in seafood Recently, naphto and naphtobacterium, which were only considered as traditional fermented foods, are starting to attract attention as feed additives for poultry, etc., but Hay bacillus As a result of feeding broilers to broilers, there are reports that it is effective up to 5 weeks, but not effective thereafter. In the early 1990s, results were already announced in the early 1990s that adding about 3% to feed promotes growth and lowers the feed requirement.

When these are added to feed, the number of harmful microorganisms such as E. coli in the intestine is extremely reduced, and beneficial microorganisms such as lactic acid bacteria are proliferated, thereby improving the growth rate and feed rate.

The feed composition according to another embodiment of the present invention is prepared by using the microorganism-based probiotic for feed.

Consumers' awareness and sales of high-quality meat and fish that are not only healthy but also tasty are increasing as their diets become more luxurious and diversified. Therefore, in response to the needs of these consumers, producers are conducting a lot of tests and research to improve the quality of meat and fish products in various ways. In particular, in order to produce high-quality meat, research on compounded feeds or feed additives is constantly in progress.

Accordingly, the present invention has the advantage of being able to improve the meat quality while increasing the growth rate of livestock and fish by beneficial microorganisms prepared as a feed composition using a microorganism-based probiotic for feed.

The livestock may be cattle, pigs, goats, sheep, horses, chickens, ducks, turkeys, etc., and preferably pigs.

The fish may be aquatic products such as flatfish, yellowtail, mackerel, and sea bream, preferably flatfish.

The feed composition may include general basic feeds fed according to the types of livestock and fish, and these basic feeds are all known in the art for their necessary components and compositions and suitable composition ratios according to the types of livestock and fish, In addition, since it is commercially available, anyone skilled in the art can very easily manufacture or purchase it and use it.

The feed composition further comprises rice husk, rice bran, tangerine juice and tangerine gourd.

The hull (hull, husk) refers to the outer husk of rice, and the inner and outer skins are densely covered with silicon, making it difficult to corrode. Its use is being expanded with compost, etc. In general, the outer skin of grain contains antioxidants that can prevent oxidation and deterioration from the outside, and flavonoid, cyanidin, phytic acid, ferulic acid, etc. are known as antioxidants contained in rice husk. In particular, isovitexin, a C-glycosyl flavonoid, showed strong antioxidant ability in rice husk (Ramarathnam N, et al. 1989. J. Agric Food Chem 37:316-319), and 2.1 to 2.4% of phytic acid present in rice husk is metal chelate. It is said to have antioxidant activity by acting as an agent.

Rice bran is also called rice bran (米糠) as the finest stencil produced when rice is milled. Rice bran obtained during the milling process of rice contains more than 40 kinds of proteins, lipids, B vitamins, minerals, fibers, and many useful trace elements. Recently, various functionalities of rice bran ingredients have been studied, and in particular, there are many reports about the anti-stress effect, anti-fatigue effect, and immune-enhancing effect of rice bran.

Fruit gourd produced after processing fruits into beverages contains various kinds of physiologically active substances. In particular, in the case of tangerine gourd, it takes a lot of money and effort to exhaust the juice by-products during the harvest season. It is true that there is no way to utilize the functional substances of discarded tangerine gourd for livestock and the functional substances are not utilized properly. In particular, hesperdin, a polyphenol component abundant in tangerines, is a physiologically active substance with antioxidant effect.

Accordingly, the present invention includes the juice of citrus fruit having excellent antioxidant power, and can be safely fed to livestock and provide a feed composition as an immune enhancer or immune function modifier.

Preferably, the feed composition of the present invention further comprises stone kasari powder and hokdol leaf powder in addition to the rice husk, rice bran, tangerine juice and tangerine meal.

Chondracanthus tenellus (Harvey) Hommersand in Hommersand is a red algae plant belonging to the order Chrysanthemum, which lives on rocks 2-4 m deep in the sea, and has a height of 5-12 cm. Cylindrical branches alternate or opposite several times, and the tip of the branch is sharp. The color is purple or reddish purple, and the round pouch is small and has no stalk. Those that grow in the shallows are small, those that grow in the depths are large. It is distributed in Japan, and in Korea, it is distributed all over the country.

Lithophyllum okamurai (Lithophyllum okamurai) is a red plant with a thickness of about 0.6mm, envelops the surface of small stones or other objects, and grows with lump-shaped projections. The whole body is covered with calcareous and has a pink or red color. The columnar protrusion corresponds to the branch, and the tip is round and the surface is smooth. In Korea, it is collected from Jeju and the southern coast.

Preferably, the feed composition contains 10 to 20 parts by weight of rice hull, 10 to 20 parts by weight of rice bran, 10 to 20 parts by weight of tangerine juice, and 5 to 10 parts by weight of orange peel based on 100 parts by weight of the microorganism-based feed probiotic of the present invention. can

More preferably, the feed composition contains 10 to 20 parts by weight of rice hull, 10 to 20 parts by weight of rice bran, 10 to 20 parts by weight of tangerine juice, 10 to 20 parts by weight of orange peel, based on 100 parts by weight of the microorganism-based feed probiotic of the present invention, It may include 5 to 10 parts by weight of stone garfish powder and 5 to 10 parts by weight of black stone leaf powder.

When used as a mixed composition within the above range, the effect of economically promoting the growth of useful microorganisms can be properly exhibited, and it can be provided as a feed composition that can further increase the efficacy, such as improving the meat quality and growth ability of livestock and fish have.

The feed additive according to another embodiment of the present invention is prepared by using the microorganism-based probiotic for feed.

Preferably, the feed composition for pigs according to another embodiment of the present invention may include the microorganism-based probiotic.

Preferably, the feed composition for flatfish according to another embodiment of the present invention may include the microorganism-based probiotic.

According to the microorganism-based feed probiotic produced according to the manufacturing method of the present invention, it is possible to increase the feed efficiency by containing beneficial microorganisms, thereby improving the growth rate and immunity of livestock and fish.

Therefore, according to the present invention, it is possible to provide a microorganism-based probiotic for feed that can improve the productivity and meat quality of livestock and fish.

1 shows the culture of Hong Guk-gyun according to an embodiment of the present invention.
2 is a graph confirming the survival rate of a microorganism-based probiotic for feed according to an embodiment of the present invention at room temperature for 4 weeks.
3 is a graph confirming the pH change of a microorganism-based probiotic for feed according to an embodiment of the present invention at room temperature for 4 weeks.
4 is a graph showing the effect of eotchijeung of Hong Guk-gyun according to an embodiment of the present invention.
5 is a photograph of halibut confirming the effect of hongguk-gyun according to an embodiment of the present invention.
6 shows the results of measuring the protein expression pattern in pig sirloin according to an embodiment of the present invention by 1-electrophoresis and silver staining.

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. However, the present invention may be embodied in several different forms and is not limited to the embodiments described herein.

[Preparation Example 1: Preparation of beneficial microorganisms]

1. Preparation of Hong Guk-Gyun Powder

culture

In order to supply sufficient oxygen to the seed strain Monascus purpureus (ATCC 34892) purchased from the BRC, 40 ml of YEME (3 g yeast extract, 5 g peptone, 3 g malt extract, 340 g sucrose, 10 g glucose, 1 g MgCl ₂ 6H) per 250 ml flask. ₂ O) 0.2% (200 μl seed per 100 ml of growth medium) was inoculated into a baffled flask containing the medium, and liquid culture was performed for 2 to 4 days.

Thereafter, using a Monascus medium and PDA agar medium, the cube-shaped Hongguk-gyun as shown in FIG. 1 was cultured for 5 to 8 days.

Preparation of Hong Guk-Gyun Powder

After performing the process of naturally drying the cultured Hongguk-gyun at room temperature of 18°C to 20°C for a long time, it was pulverized into a powder of 10 mesh or more in a completely dried state to prepare Hongguk-gyun powder (MG).

2. Preparation of other microbial powders

Saccharomyces cerevisiae was used as the yeast, and photosynthetic bacteria, Rhodobacter capsulatus and Hwangguk bacterium, were prepared using the same method as in the preparation of the honggukgyun powder, photosynthetic bacterial powder (RG), Hwanggukgyun powder (AG) and yeast powder (YG) were mixed as shown in Table 1 below to prepare a microorganism-based probiotic for feed.

BF1 BF2 BF3 BF4 BF5 BF6 MG 100 100 100 100 100 100 RG - 20 30 40 50 60 AG - 20 30 40 50 60 YG - 20 30 40 50 60

(Unit: parts by weight)

[Experimental Example 1: Confirmation of viability and pH of microbial-based feed probiotics]

cytotoxicity

In order to test the toxicity of the microbial-based feed probiotics BF1 to BF6 prepared in Preparation Example 1, a difference in toxicity and side effects when the probiotic was administered was confirmed in a repeated-dose toxicity test in rats.

Six-week-old male and female rats of SD opening were divided into 10 rats per group (5 males and females each) and BF1 to BF6 were administered, and each probiotic was dissolved in 0.5% MC solution and then orally administered once every day at the same time in the morning 13 Repeat for a week.

A single dose was administered in an amount of 3.75 mg/kg to 5 mg/kg. Afterwards, mortality, general symptoms, weight change, and feed and water intake were observed.

As a result, no deaths occurred within the experimental period. In light of the above experimental results, it was confirmed that the microbial-based feed probiotics BF1 to BF6 prepared in Preparation Example 1 did not pose a problem in toxicity.

Check viability and pH

While the microorganism-based feed probiotics BF1 to BF6 were stored at room temperature for 4 weeks, the viability of the probiotics according to each range was checked by the CFU counting method, and each pH was measured by a pH meter (pH/ISE Meter 735P, Istek Co., Ltd, Seoul, Korea).

The results are shown in FIGS. 2 and 3 below, and it was confirmed that, in the case of a probiotic in the range of BF3 to BF5, the viability was maintained remarkably high (FIG. 2).

In addition, probiotics in the range of BF1 and BF2 showed pH 6.5 and pH 6.0, and in the case of probiotics in the range of BF6, pH higher than 9.0, whereas the pH of probiotics in the range of BF3 to BF5 was confirmed to appear stably with almost no change (Fig. 3). In particular, BF4 showed the most stable pH at pH 7.8.

[Preparation Example 2: Preparation of feed composition]

Among the microorganism-based feed probiotics prepared in Preparation Example 1, rice husk, rice bran, tangerine juice, tangerine meal, stone gaseous powder and black stone leaf powder were included in the probiotic according to the range of BF4 having high viability and the most stable pH. A feed composition was prepared with the same composition as in 2.

MP1 MP2 MP3 MP4 MP5 BF4 100 100 100 100 100 chaff - 5 10 20 30 rice bran - 5 10 20 30 Tangerine juice - 5 10 20 30 tangerine gourd - 5 10 20 30 stonefish powder - - 5 10 20 Black Stone Leaf Powder - - 5 10 20

(Unit: parts by weight)

[Experimental Example 2: Feed efficiency improvement effect test]

1. Eostasis due to the addition of Hong Guk-gyun

Halibut (float) weighing 5 kg was stocked in a water tank to check for fish body weight using honggukgyun powder MG (BF1) prepared by using the honggukgyun powder MG (BF1) of the present invention. They were divided into non-control groups and grown for 10 days.

The results are shown in Table 3, Figures 4 and 5 below.

division control experimental group starting average weight 5 6 Average weight at the end of the experiment 13.5 15.7 average weight gain 9 10 Average daily weight gain per couple 0.9(100) 1.67 (186)

(Unit: Kg)

As shown in Table 3, the control group without addition was 0.9 kg, and it was confirmed that the daily weight gain increased by 186% compared to 1.67 kg of the experimental group administered with honggukgyun powder.

2. Increase in weight gain

The feed compositions MP1 to MP5 prepared in Preparation Example 2 were mixed with the basic feed as shown in Table 4 below and fed to the pigs. Basic feed is a feed that contains corn, wheat, wheat hull, soft berry meal, and rapeseed meal as main raw materials.

division salary feed control basic feed Experimental group 1 Basic feed + feed composition of the present invention MP1 Experimental group 2 Basic feed + feed composition of the present invention MP2 Experimental group 3 Basic feed + feed composition of the present invention MP3 Experimental group 4 Basic feed + feed composition of the present invention MP4 Experimental group 5 Basic feed + feed composition of the present invention MP5

Feed was allowed to be vegetarian, and water was freely drinkable using an automatic drinker. Feeding management was carried out according to the general breeding management method, and the pig farm area and water supply facilities were the same between the control group and the experimental group. The experimental period was 5 weeks, and the daily gain, daily feed intake and feed efficiency (gain/feed intake) were calculated based on the start and end of the experiment.

The results are shown in Table 5 below.

division Daily weight gain (Kg) Daily feed intake (g) Feed efficiency control 0.408±0.047 0.743±0.062 0.549±0.026 Experimental group 1 0.462±0.055 0.735±0.042 0.628±0.034 Experimental group 2 0.469±0.052 0.742±0.048 0.632±0.040 Experimental group 3 0.482±0.033 0.748±0.042 0.644±0.037 Experimental group 4 0.501±0.018 0.752±0.070 0.666±0.044 Experimental group 5 0.472±0.043 0.738±0.049 0.639±0.046

The results in Table 5 show that when the feed composition MP1 to MP5 is added to the basic feed and fed, the feed efficiency increases by approximately 8 to 9%. And in the case of experimental group 1, experimental group 2, and experimental group 5 in which MP1, MP2 and MP5 were mixed with basic feed, the feed efficiency showed a slight increase, but no other significant difference was found. In the case of 3 and experimental group 4, it was confirmed that the feed efficiency showed a tendency to significantly increase.

3. Antioxidant effect

The antioxidant effect was measured in order to compare the effect on immunity according to the feed composition MP1 to MP5 of the present invention. The antioxidant effect was measured by the electron donating ability (DPPH, (1,1-diphenyl-2-picrylhydrazyl)) assay method.

The results are shown in Table 6 below.

division DPPH (%) Antioxidant activity increase rate (%) control 120 Experimental group 1 437 Experimental group 2 559 Experimental group 3 832 Experimental group 4 995 Experimental group 5 499

Compared to the feed of the control group, the antioxidant activity was increased in the fed feeds of Experimental Group 1, Experimental Group 2, and Experimental Group 5 to which the MP1, MP2, and MP5 feed compositions were added. In case 4, it was confirmed that the antioxidant activity was significantly increased.

[Experimental Example 3: Meat quality improvement effect]

First, six pigs were selected by classifying them into pork sirloin with good meat quality and low grade, and the expression patterns of proteins expressed from pig muscle tissue (loin) for each grade were confirmed. One-dimensional electrophoresis (1Dimensionelectrophoresis) and silver staining (Silver staining) were used to measure the protein expression pattern after electrophoresis in two types according to the acrylamide %, the results are shown in FIG.

As shown in Figure 6, the protein expression pattern in pig muscle tissue (loin) by grade was HQLD (high quality of longissimus dorsi (1-6)) for pork with good meat quality, and LQLD for pork with low meat quality ( When classified as low quality of longissimus dorsi (7-12)), it was confirmed that the proteins were differentially expressed according to grade at 130kDa(a*) and 34kDa(b#), respectively.

Therefore, based on this, the protein expression patterns of the proteins expressed in the pig sirloin of Experimental Groups 1 to 5 according to Example 2 were measured in the same manner as above. Comparative analysis of protein HQLD (high quality of longissimus dorsi (1-6)) with good meat quality and protein LQLD (low quality of longissimus dorsi (7-12)) from pork loin with low meat quality in Table 7 For relative comparison, pigs with good meat quality in which the HQLD protein was expressed were set as index 5, and the protein expression patterns of experimental groups 1 to 5 were evaluated as indices 1 to 10.

It will be said that the higher the index, the better the protein expression pattern.

good quality pork Experimental group 1 Experimental group 2 Experimental group 3 Experimental group 4 Experimental group 5 HQLD 5 6 7 8 8 7 LQLD 0 2 One 0 2 2

(Unit: Index)

As shown in Table 7, compared with the protein expression pattern of pigs with good meat quality, it was confirmed that the same or more proteins were expressed in Experimental Groups 1 to 5.

In particular, in the case of experimental group 3 and experimental group 4 in which MP3 and MP4 were mixed with basic feed, the protein expression pattern was highly evaluated, and it was confirmed that the meat quality was improved.

Although preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention as defined in the following claims are also provided. is within the scope of the

Claims (3)

Hong Guk-Gyun culturing step of culturing Hong Guk-Gun (Monascus purpureus) in a liquid phase;
A powdering step of drying and pulverizing the cultured honggukgyun;
30 to 50 parts by weight of photosynthetic bacteria powder based on 100 parts by weight of Hong Guk-gyun that has undergone the powdering step; Preparing a mixed microorganism by mixing 30 to 50 parts by weight of Hwanggukgyun powder and 30 to 50 parts by weight of yeast powder; and
Based on 100 parts by weight of the mixed microorganism, 10 to 20 parts by weight of rice husk; Rice bran 10 to 20 parts by weight; 10 to 20 parts by weight of tangerine juice; Mandarin orange peel 10 to 20 parts by weight; A step of preparing a probiotic composition by mixing 5 to 10 parts by weight of stone kale powder and 5 to 10 parts by weight of porcini powder
A method for producing a probiotic composition for feed that improves the growth rate or immunity. The method of claim 1,
The powdering step
The powder is pulverized to have a particle size of 10 mesh or more.
A method for producing a probiotic composition for feed that improves the growth rate or immunity. The method of claim 1,
The photosynthetic bacteria are Rhodobacter sphaeroides (R. sphaeroides), Rhodobacter apigmentum (R. apigmentum), Rhodobacter azotoformans (R. azotoformans), Rhodobacter blasticus (R. blasticus), Rhodobacter gluconicum (R. gluconicim), Rhodobacter litoralis (R. litoralis), Rhodobacter masiliensis (R. massiliensis), Rhodobacter veldkampii (R. veldkampii) and mixtures thereof. which is selected from
A method for producing a probiotic composition for feed that improves the growth rate or immunity.

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