KR20210012578A - Pouch for Oral Cavity Comprising Acmella oleracea Extracts - Google Patents

Abstract

The present invention relates to a composition including an active ingredient having an effect of sterilizing microorganisms causing bad breath. The present invention provides a pouch for oral cavity including a Paracres (Acmella oleracea) extract. The pouch of the present invention has a strong antibacterial effect. The pouch is safe for human use, and the pouch for oral cavity has excellent flavor due to the unique taste and smell of Paracres.

Description

Pouch for Oral Cavity Comprising Acmella oleracea Extracts containing paracres extract

The present invention relates to an oral pouch containing a paracres extract, and more particularly, to an oral pouch comprising an ethanol extract of paracres flowers.

The occurrence of halitosis, which is pointed out as a representative oral disease along with tooth decay, has caused a lot of inconvenience to humans in social life since human history, and it is tentatively estimated that more than 50% of Korean adults are suffering from bad breath problem. Is being reported.

Experts report that 85-90% of the causes of bad breath are due to poor oral hygiene, and bad breath is mainly caused by microorganisms attached to plaque and food debris attached to the inside of the tongue. Alcohols, volatile sulfur compounds, lower fatty acids, amino acids, and ammonia are attracting attention.

In particular, volatile sulfur-containing compounds (VSC) such as hydrogen sulfide, methyl mercaptan, and dimethyl disulfide account for more than 90% of bad breath, and among them, methyl mercaptan. ) Has a so-called rotted onion-like odor and is known to have a high correlation between its concentration and the intensity of bad breath.

It is known that there are more than 250 kinds of bacteria in the oral cavity, and it is mainly anaerobic bacteria that are involved in bad breath, but the temperature and moisture in the mouth are both a pleasant habitat for bacteria. Saliva, dietary residues, detached epithelial cells, Protein components such as white blood cells are decomposed to produce odor components.

These protein components are decomposed by bacteria in the oral cavity having protein-degrading ability to release disulfides, which are reduced to thiols and converted into volatile sulfur compounds.

Until now, efforts have been made to continuously remove the main causative agent of bad breath or to kill the causative bacteria, but no great results have been achieved. As a representative example, various types of mouth washes have been developed and used for the purpose of removing bad breath, but most of them contain alcohol for sterilization purposes, so there is a problem that severely induces dry mouth.

If the occurrence of bad breath is harmful to periodontal disease, respiratory disease, digestive system disease, etc., it is necessary to completely cure the disease that causes it, but especially those who do not have bad breath due to these diseases have physiological bad breath.

In addition, although the seriousness of bad breath is recognized in common, the development of food for relieving it is mainly limited to the development of gum and candy containing masking flavor, regardless of developed and underdeveloped countries.

According to several recent reports, oxygen-saturated beverages have been reported to help remove bad breath, and there are reports that chlorophyll-based food additives have excellent deodorizing effects. In addition, it was reported that toothpaste with kojic acid significantly reduced the strength of methylmercaptan.

Looking at the patent literature related to the removal of bad breath, KR Patent Application No. 10-2003-0066175 (name of the invention: a film for suppressing and removing bad breath and a film-forming composition for producing the same) includes green tea extract, quince extract, and citron extract. And it discloses a film for suppressing and removing bad breath, characterized in that the extract selected from the group consisting of a mixture thereof is uniformly dispersed in the water-soluble film substrate as an active ingredient,

KR Patent Application No. 10-2001-0008502 (name of the invention: method of extracting substances for anti-odor) discloses a composition for suppressing bad breath, including mugwort extract, pine needle extract, and pine needle sprout extract as anti-odor substances.

However, in the case of the above-described composition for removing bad breath, the antibacterial activity of removing the bacteria causing bad breath is excellent, but the unique flavor of the extract is strong, so there is a problem in that the mouth feels thick after use.

Throughout this specification, a number of papers and patent documents are referenced and citations are indicated. The disclosure contents of the cited papers and patent documents are incorporated by reference in this specification as a whole, and the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly described.

The inventors of the present invention have made intensive research efforts to develop a composition for removing bad breath having excellent antibacterial activity and excellent bad breath removal effect, as well as excellent flavor. As a result, when the composition for removing bad breath is completed using the ethanol extract of Paracres ( Acmella oleracea ), the antibacterial activity is strong, so the effect of removing bad breath is excellent, and it is safe for human use, as well as the unique taste and fragrance of Paracres. The present invention was completed by discovering the fact that the flavor can be excellent.

Accordingly, an object of the present invention is to provide a composition for removing bad breath comprising a paracres extract.

In addition, another object of the present invention is to provide a liquid cigarette composition for removing bad breath comprising a paracres extract.

In addition, another object of the present invention is to provide an oral pouch for removing bad breath comprising a paracres extract.

Other objects and advantages of the present invention will become more apparent by the following detailed description, claims and drawings.

The present invention provides a bad breath removal composition.

The inventors of the present invention have made intensive research efforts to develop a composition for removing bad breath having excellent antibacterial activity and excellent bad breath removal effect, as well as excellent flavor. As a result, when the composition for removing bad breath is completed using the ethanol extract of Paracres ( Acmella oleracea ), the antibacterial activity is strong, so the effect of removing bad breath is excellent, and it is safe for human use, as well as the unique taste and fragrance of Paracres. It has been confirmed that the flavor of the can be excellent.

According to an aspect of the present invention, the present invention provides a composition for removing bad breath comprising an active ingredient having a bactericidal effect of microorganisms causing bad breath, wherein the active ingredient is Paracres ( Acmella oleracea) It provides a composition for use.

The term “paracres” used herein may mean a perennial plant or perennial plant of the chrysanthemum family.

The term “oral pouch” as used herein may mean any pouch that is held in the mouth and spit out after a certain period of time elapses.

The term “wasabi” as used herein may mean a perennial herb belonging to the Brassicaceae family.

The term “applemint” used in the present specification may mean a perennial plant of the Lamiaceae family of the molar plant.

As a result of the KFDA Food Ingredient Search ( http://fse.foodnara.go.kr ), the Paracres has no problem in legally using it as a pharmaceutical composition or food composition, and has been known as a plant that has excellent effects on blood circulation, etc. In view of the points, it can be used in food or pharmaceutical compositions that exhibit joint pain relief and inflammation relief.

According to a preferred embodiment of the present invention, the paracres may be preferably selected from the group including flowers, leaves, stems, fruits, seeds, roots and outposts of paracres, more preferably flowers or leaves, and , Most preferably may be a flower.

According to a preferred embodiment of the present invention, the extraction solvent of the extract of the present invention is preferably from the group comprising water, ethanol, methanol, methylene chloride, ethyl acetate, butanol, hexane, butylene glycol, chloroform, and mixtures thereof. It may be selected, more preferably water or ethanol, and most preferably ethanol.

In the present specification, the term “extract” used while referring to the extract of paracres, wasabi, and applemint is formulated so that the plant itself can be ingested as well as the extraction result obtained by treating the aforementioned plant with an extraction solvent (for example, Powdered) has a meaning to include the processed product.

If the extract used in the composition of the present invention is obtained by treating the plant with an extraction solvent, various extraction solvents may be used. Preferably, (a) water, (b) an anhydrous or hydrous lower alcohol having 1-4 carbon atoms (e.g. methanol, ethanol, propanol, butanol, normal-propanol, iso-propanol and normal-butanol, etc.), (c) A mixed solvent of the lower alcohol and water, (d) acetone, (e) ethyl acetate, (f) chloroform, (g) 1,3-butylene glycol, (h) hexane, (i) diethyl ether, or (j) An extract can be obtained by treating the plant with butyl acetate.

As used herein, the term "extract" has the meaning commonly used as a crude extract in the art as described above, but broadly includes a fraction obtained by further fractionating the extract. That is, the plant extract includes not only those obtained using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, fractions obtained by passing the extract through an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through the purification method are also included in the plant extract of the present invention.

Paracres, horseradish, or applemint extract used in the present invention may be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

According to a preferred embodiment of the present invention, the composition for removing bad breath comprising the paracres extract of the present invention may preferably contain 0.1-5% by weight of the paracres ethanol extract based on the total weight of the composition.

When the content of the paracres ethanol extract is less than 0.1% by weight, there is a problem that the deodorizing effect is insignificant and the palatability of the prepared gargle composition is poor, and when the content of the paracres ethanol extract exceeds 5% by weight, the composition is irritated. Of course, there may be a problem of an increase in manufacturing cost.

According to a preferred embodiment of the present invention, the composition for removing bad breath comprising the paracres extract of the present invention may further include 2-5% by weight of menthol based on the total weight of the composition.

In the present invention, it is very important to further include menthol. This is because, when a certain amount of menthol is additionally included in addition to the paracres ethanol extract, palatability of the gargle composition increases, as well as the use of expensive paracres extract can be reduced.

According to a preferred embodiment of the present invention, the composition for removing bad breath comprising the paracres extract of the present invention may further include 1-2% by weight of propolis based on the total weight of the composition.

In the present invention, it is very important to further include propolis. Because, when a certain amount of propolis is additionally included in addition to the paracres ethanol extract, even when a small amount of expensive paracres extract is used, the deodorizing effect of the gargle composition can be maximized, as well as the palatability of the gargle composition can be improved. Because there is.

According to a preferred embodiment of the present invention, the composition for removing bad breath comprising the paracres extract of the present invention may further comprise 1-2% by weight of wasabi root extract based on the total weight of the composition. .

In the present invention, it is a very important composition to further include the wasabi root extract. Because, when a certain amount of wasabi root extract is additionally included in addition to the paracres ethanol extract, the deodorizing effect of the gargle composition can be maximized even when a small amount of the expensive paracres extract is used, as well as the palatability of the gargle composition. Because it can be increased.

According to a preferred embodiment of the present invention, the composition for removing bad breath comprising the paracres extract of the present invention may further include 1-2% by weight of an applemint extract based on the total weight of the composition.

In the present invention, it is a very important composition to further include the applemint extract. This is because, as a result of a number of experiments, it was found that palatability of the gargle composition can be improved when a certain amount of applemint extract is additionally included in addition to the paracres ethanol extract.

According to another aspect of the present invention, in a composition comprising an active ingredient having a bactericidal effect of microorganisms causing bad breath, the active ingredient is an electronic cigarette liquid composition comprising a paracres extract which is a paracres ( Acmella oleracea) extract. to provide.

In the present specification, since the contents of the liquid composition are in common with the composition for removing bad breath described above, the description thereof will be omitted to prevent the present specification from becoming excessively complicated.

The features and advantages of the present invention are summarized as follows:

(a) The present invention provides an oral pouch comprising an active ingredient having a bactericidal effect of microorganisms causing bad breath, wherein the active ingredient is an oral pouch comprising a paracres extract, which is an extract of Acmella oleracea .

(b) The pouch of the present invention has a strong antibacterial effect, so it has an excellent halitosis removal effect, is safe for human use, and provides excellent flavor of an oral pouch for removing bad breath due to the unique taste and aroma of Paracres.

1 shows the appearance of paracres ethanol extract.
Figure 2 shows the appearance of the finished oral cleaning composition.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for describing the present invention in more detail, and that the scope of the present invention is not limited by these examples according to the gist of the present invention, for those of ordinary skill in the art to which the present invention belongs. It will be self-evident.

Example

Throughout this specification, the “%” used to indicate the concentration of a specific substance is (weight/weight)% for solids/solids, (weight/volume)% for solids/liquids, and Liquid/liquid is (vol/vol) %.

Preparation: Preparation of a composition for removing bad breath containing paracres extract

Preparation 1: Preparation of paracres extract

Preparation 1-1: Preparation of paracres ethanol extract

1000 g of paracres flowers were washed several times with a washer so that there were no foreign substances using purified water, and dried using a dryer. After that, put the flower of Paracres in an extractor and repeatedly extract 4 times at 70~75℃ for 24 hours using 6 times (v/w) of alcohol (97%) of the weight of the flower of Paracres as an extraction solvent. I did. The flower extract of Paracres was collected and filtered using a filtration filter (5 μm), and then the filtrate was transferred to a concentrator and concentrated under reduced pressure for about 3-4 hours under conditions of 45°C, 700-750 mmHg to contain more than 60% solids. A flower concentrate was prepared (solid content of 60% or more), and the concentrate was freeze-dried, and then pulverized to prepare a paracres ethanol extract.

Preparation 1-2: Preparation of paracres hot water extract

1000 g of paracres flowers were washed several times with a washer so that there were no foreign substances using purified water, and dried using a dryer. Next, the dried paracres flowers were each put into an extractor and extracted twice in succession at 120 degrees Celsius for 3 hours and 30 minutes using water 6 times the weight of the paracres flowers as an extraction solvent. The paracres flower extract was collected and filtered using a filtration filter (5 µm), and then the filtrate was transferred to a vacuum concentrator and concentrated under reduced pressure at 120 degrees Celsius for about 3-4 hours to prepare a medicinal concentrate having a solid content of 60% or more. Thereafter, the herbal concentrate was rapidly cooled to -60 degrees Celsius within 8 hours after sterilization, and then freeze-dried and pulverized to -80 degrees Celsius to prepare a Paracres hot water extract.

Preparation 2: Preparation of gargle composition

A gargle composition was prepared using the paracres flower extract prepared in Preparation 1. The specific composition is shown in Table 1 below.

ingredient Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Fluoride-containing drugs 0.1 0.1 0.1 0.1 0.1 0.1 Citric acid 3 3 3 3 3 3 Preparation 1-1 extract 2 - 2 2 2 2 Preparation 1-2 extract - 2 - - - - Wasabi extract One One One One One - Apple Mint Extract One One One One - One Propolis extract One One One - One One Menthol 2 2 - 2 2 2 Purified water Balance Balance Balance Balance Balance Balance

Unit: wt%

Preparation 3: Preparation of liquid tobacco composition

A liquid tobacco composition was prepared using the paracres flower extract prepared in Preparation 1. The specific composition is shown in Table 2 below.

ingredient Example 7 Example 8 Example 9 Example 10 Example 11 Example 12 Vegetable glycerin 35 35 35 35 35 35 Pure nicotine 0.04 0.04 0.04 0.04 0.04 0.04 Propylene glycol 25 25 25 25 25 25 Preparation 1-1 extract 5 - 3 3 3 3 Preparation 1-2 extract - 5 - - - - Wasabi extract - - One 1.5 1.5 - Apple Mint Extract - - One 1.5 - 1.5 Propolis extract - - One - 1.5 1.5 Menthol flavor premix Balance Balance Balance Balance Balance Balance

Unit: wt%

Preparation 4: Preparation of oral pouch

An oral pouch was prepared using the Paracres flower extract prepared in Preparation 1. The specific composition is shown in Table 3 below. The oral pouch was prepared using a polyester fabric for tea bags. The pouch was adhered using an ultrasonic thermal fusion method, and the pouch size was 2 cm wide and 1 cm long, and the raw materials shown in Table 3 below were filled inside the pouch, and then the pouch for an oral cavity was ultrasonically fused.

ingredient Example 13 Example 14 Example 15 Example 16 Example 17 Example 18 Preparation 1-1 extract 5 - 3 3 3 3 Preparation 1-2 extract - 5 - - - - Wasabi extract - - One 1.5 1.5 - Apple Mint Extract - - One 1.5 - 1.5 Propolis extract - - One - 1.5 1.5 Crushed dried green tea leaves Balance Balance Balance Balance Balance Balance

Unit: wt%

Experimental Example: Checking the efficacy of a composition for removing bad breath containing paracres extract

Experimental Example 1: Confirmation of deodorant effect

To measure the deodorizing effect of the paracres ethanol extract prepared by the method of Preparation 1-1 and the paracres hot-water extract prepared by the method of Preparation 1-2, which is a representative source of bad breath, methyl mercaptan Paracres ethanol extract prepared by the method of Preparation 1-1, and 0.9 ml of the extract of the Paracres hot water extract prepared by the method of Preparation 1-2 were mixed with 0.1 ml of methylmercaptan and put into a 5 ml vial, and then at 37 degrees Celsius 3 After incubation for a minute, 1 ml was taken from the upper layer and injected into the GC to measure the concentration of methylmercaptan remaining in the vial upper part. At this time, the GC analysis conditions and the method of calculating the deodorization rate are shown in Table 4 below.

Deodorization rate (%) = (CS)/CX 100
C: Peak area of methylmercaptan when methylmercaptan is added to water
S: Peak area of methylmercaptan when added to the extract of the paracres ethanol extract prepared by the method of Preparation 1-1 and the paracres hot water extract prepared by the method of Preparation 1-2 Inlet: Splitless
Inlet temp. : 250℃
Carrier gas: Helium
Detector: FID
Detector temp. : 230℃
Initial temp. : 35℃ to 70℃ at 2℃/min., 70℃ to 100℃ at 5℃/min.

As a result of the experiment, the deodorization rates for methylmercaptan of the paracres ethanol extract prepared by the method of Preparation 1-1 and the paracres hot-water extract prepared by the method of Preparation 1-2 were 96%, respectively, and 93%. As a comparative example, green tea extract was used, and in the case of green tea extract, the deodorization rate for methyl mercaptan was 65%.

Experimental Example 2: palatability test-gargle composition

In the preference evaluation, the taste palatability of the gargle composition prepared by the method of Examples 1 to 6 was investigated, and 15 employees who entered the Ansan Startup Academy were selected and carried out for the corresponding personnel. The gargle composition prepared by the method of Examples 1 to 6 was used as the target, and after the demonstration, the palatability of each treatment was evaluated and recorded in 10 steps from 1 to 10. At the time of the demonstration, 20 ml of a gargle composition prepared by the method of Examples 1 to 6 was provided. Gagrin, which can be purchased at an internet shopping mall, was used as a control.

The acceptability of the gargle composition prepared by the method of Examples 1 to 6 is shown in Table 5 below. In summary, the preference was evaluated in the order of Example 1, Example 6, Example 5, Example 4, Example 3, Example 2, and control of the present invention. That is, in terms of palatability, the sample corresponding to Example 1 obtained the highest preference, and the numerical value also showed a meaningful level of significance compared to the rest of the Examples and the control. This proves that the method of manufacturing the method and ratio presented in the present invention has superior effects compared to other manufacturing methods.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Control Palatability 8.51 6.92 7.17 7.67 7.89 7.97 6.53 1.21 0.98 1.12 1.79 1.32 1.23 0.89

For each item, the upper column is the mean value, and the lower column is its standard deviation

Experimental Example 3: palatability test-electronic cigarette liquid composition

In the preference evaluation, the flavor palatability of the liquid e-cigarette composition prepared by the method of Examples 7 to 12 was investigated, and 15 smokers were selected among the executives and staff members who entered the Ansan Startup Academy and conducted targeting the corresponding personnel. The e-cigarette liquid composition prepared by the method of Examples 7 to 12 was used as a target, and after the demonstration, the palatability of each treatment was evaluated and recorded in 10 steps from 1 to 10. At the time of the demonstration, 3 ml of the e-cigarette liquid composition prepared by the method of Examples 7 to 12 and an e-cigarette were provided. As a control, 3 ml of a general menthol flavor liquid composition (same pure nicotine content) and an electronic cigarette that can be purchased at an internet shopping mall were provided.

The acceptability of the gargle composition prepared by the method of Examples 7 to 12 is shown in Table 6 below. In summary, preference was evaluated in the order of Example 7, Example 12, Example 10, Example 11, Example 9, Example 8, and control of the present invention. That is, in terms of palatability, the sample corresponding to Example 7 obtained the highest preference, and the numerical value also showed a meaningful level of significance compared to the rest of the Examples and the control. This proves that the method of manufacturing the method and ratio presented in the present invention has superior effects compared to other manufacturing methods.

Example 7 Example 8 Example 9 Example 10 Example 11 Example 12 Control Palatability 8.61 6.92 6.97 7.93 7.13 8.01 6.62 1.12 1.08 1.21 1.49 1.24 1.32 1.12

For each item, the upper column is the mean value, and the lower column is its standard deviation

Experimental Example 4: palatability test-oral pouch

In the preference evaluation, the flavor palatability of the oral pouch manufactured by the method of Examples 13 to 18 was investigated, and 15 employees who entered the Ansan Entrepreneurship Academy were selected and carried out for the corresponding personnel. The oral pouch prepared by the method of Examples 13 to 18 was used as a target, and after the demonstration, the palatability of each treatment tool was evaluated and recorded in 10 steps from 1 to 10. At the time of the demonstration, one piece of oral pouch prepared by the method of Examples 13 to 18 was provided. As a control, pulverized dry green tea powder was filled in the same pouch and provided.

The preference diagram of the oral pouch prepared by the method of Examples 13 to 18 is shown in Table 7 below. In summary, preference was evaluated in the order of Example 13, Example 18, Example 17, Example 16, Example 15, Example 14, and control of the present invention. That is, in terms of palatability, the sample corresponding to Example 13 obtained the highest preference, and the numerical value also showed a meaningful level of significance compared to the rest of the Examples and the control. This proves that the method of manufacturing the method and ratio presented in the present invention has superior effects compared to other manufacturing methods.

Example 13 Example 14 Example 15 Example 16 Example 17 Example 18 Control Palatability 8.32 6.72 6.87 7.12 7.23 7.88 3.97 1.23 0.98 1.24 1.09 1.28 0.98 1.13

For each item, the upper column is the mean value, and the lower column is its standard deviation

Claims (10)

In the oral pouch containing an active ingredient having a sterilizing effect of microorganisms causing bad breath,
The active ingredient is Paracres ( Acmella oleracea) extract
Oral pouch containing paracres extract.
The method of claim 1,
The extraction solvent of the extract is water, ethanol, methanol, methylene chloride, ethyl acetate, butanol, hexane, butylene glycol, chloroform, and oral pouch comprising a paracres extract, characterized in that selected from the group containing a mixture thereof .
The method of claim 2,
Oral pouch containing paracres extract, characterized in that the extraction solvent of the extract is ethanol.
The method of claim 3,
The paracres is an oral pouch comprising a paracres extract, characterized in that selected from the group comprising paracres flowers, leaves, stems, fruits, seeds, roots and outposts.
The method of claim 4,
The paracres is an oral pouch comprising a paracres extract, characterized in that the flowers of paracres.
The method of claim 5,
An oral pouch comprising a paracres extract, characterized in that the composition for removing bad breath comprising the paracres extract comprises 0.1-5% by weight of the paracres ethanol extract based on the total weight of the composition.
The method of claim 6,
Oral pouch comprising a paracres extract, characterized in that the composition for removing bad breath comprising the paracres extract further comprises 2-5% by weight of menthol based on the total weight of the composition.
The method of claim 6,
An oral pouch comprising a paracres extract, characterized in that the composition for removing bad breath comprising the paracres extract further comprises 1-2% by weight of propolis based on the total weight of the composition.
The method of claim 8,
The composition for removing bad breath comprising the paracres extract is an oral pouch comprising a paracres extract, characterized in that it further comprises 1-2% by weight of a hot water extract of horseradish root based on the total weight of the composition.
The method of claim 9,
The composition for removing bad breath comprising the paracres extract is an oral pouch comprising a paracres extract, characterized in that it further comprises 1-2% by weight of the epimint extract based on the total weight of the composition.

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