KR20200145340A - Composition for skin whitening comprising ulmus pumila extract - Google Patents

Abstract

The present invention relates to a composition for skin whitening comprising a Ulmus pumila extract as an active ingredient. It has been confirmed that the Ulmus pumila extract exhibits an effect of inhibiting tyrosinase activity that induces pigmentation, and thus the composition containing the Ulmus pumila extract as an active ingredient may be provided as a health food or cosmetic composition for skin whitening.

Description

Composition for skin whitening comprising ulmus pumila extract}

The present invention relates to a composition for skin whitening containing a bisul tree extract as an active ingredient.

Recently, as interest in healthy skin has increased, research on cosmetics made of natural substances is being actively conducted.In particular, prevention of various skin problems such as inflammatory acne or skin diseases such as pigmented melanoma and freckle And interest in treatment is increasing.

The skin consists of the epidermis, the dermis, and the hypodermis, and in the dermis, the extracellular matrix (ECM) consisting of collagen and elastin, etc. fibroblast).

Collagen is a major insoluble fibrous protein in the extracellular matrix (ECM) and connective tissue, and its main function is to maintain skin elasticity. There are 16 types of collagen, and about 80-90% of the collagen in the human body is composed of types I, II, and III. Type I collagen accounts for about 75-80% of collagen in normal skin, and bone It forms more than 90% of the organic mass, is a major collagen in ligaments, corneas and many interstitial connective tissues, is a major ECM component of the skin, and is responsible for structural integrity.

Most of the type Ⅰ collagen, type III collagen is contained in a small amount, and contains 3-4% of elastin, which gives elasticity to the skin. Most of the collagenases belong to the matrix metalloproteinases (MMPs) group and cause collagen degradation by breaking peptide bonds. Such skin aging is caused not only by accumulation of melanin but also by accumulation of reactive oxygen species (ROS). Harmful reactive oxygen species that cannot be removed by the body's antioxidant enzyme system damage cells, lipids, proteins, DNA, etc., destroying major components of cells and dermal layers, causing wrinkles and loss of elasticity.

Melanin present in the epidermis of the skin is one of the important factors determining skin color, and the apparent skin color is determined by the amount and distribution of melanin pigments. When ultraviolet rays are irradiated, melanin is produced through melanogenesis in melanocytes to protect the skin, and the skin color is changed to black by the generated melanin, resulting in pigmentation.

Tyrosinase is an enzyme that causes the first stage of melanogenesis, and tyrosine is converted to dopaquinone via dopa (3,4-dihydroxyphenilalanine (DOPA)). Through an enzymatic reaction, melanin, a dark brown copolymer, is produced through DOPA chrome.

Currently, in the development of whitening agents, a method of decolorizing by reducing the produced melanin pigment and a method of inhibiting the activity of tyrosinase, an enzyme that forms melanin pigment, are known. The produced melanin deposits pigmentation on the skin to form spots and freckles, and may also cause skin cancer.

Accordingly, there is an urgent need to develop natural materials for maintaining healthy skin, such as inhibiting skin aging and pigmentation.

Republic of Korea Patent Publication No. 10-2014-0104142 (published on August 28, 2014)

An object of the present invention is to provide a composition containing a bisul tree extract as an active ingredient as a natural material for maintaining healthy skin by inhibiting skin pigmentation.

The present invention provides a cosmetic composition for skin whitening containing a bisul tree extract as an active ingredient.

In addition, the present invention provides a health food for skin whitening containing a bisul tree extract as an active ingredient.

According to the present invention, as it was confirmed that the bisul tree extract exhibits an effect of inhibiting the tyrosinase activity that induces pigmentation, a composition containing the bisul tree extract as an active ingredient is used as a cosmetic composition or health food for skin whitening. Can provide.

1 is a result of confirming the total polyphenol and flavonoid content of the bisul tree methanol extract (UPME), and the value after 3 repeated experiments was shown as mean ± SD, and tannic acid and rutin were respectively used for quantification of the total polyphenol and flavonoid content. The calibration curve was shown using.
Figure 2 is a result of confirming the electron donating ability of the methanol extract (UPME) of bisulnamu, the value after repeated experiments 3 times was shown as mean ± SD, AA is ascorbic acid.
Figure 3 is a result of confirming the inhibitory effect of the collagenase activity of the methanol extract (UPME) bisulnamu, and the value after repeated experiments 3 times was shown as mean ± SD, AA is ascorbic acid.
Figure 4 is a result of confirming the inhibitory effect of the elastase activity of the methanol extract (UPME) of Bisul tree, the value after repeated experiments 3 times was shown as mean ± SD, AA is ascorbic acid.
5 is a result of confirming the mushroom tyrosinase activity inhibitory effect of the bisulnamu methanol extract (UPME), the values after repeated experiments 3 times are shown as mean ± SD.

Hereinafter, the present invention will be described in more detail.

The present invention can provide a cosmetic composition for skin whitening containing a bisul tree extract as an active ingredient.

The bisul tree extract may be extracted with water, C1 to C4 alcohol, or a mixed solvent thereof. More preferably, it may be a methanol extract, but is not limited thereto.

The bisul tree extract may be one that inhibits the activity of tyrosinase, collagenase, or elastase.

According to an embodiment of the present invention, in order to confirm the anti-wrinkle effect of the extract of the bisul tree, collagenase and elastase that induce skin wrinkles by decomposing ascorbic acid as a positive control and collagen and elastin as elastic fibers are used. As a result of confirming each activity inhibitory effect by treatment, the collagenase activity inhibitory activity of the methanol extract of ascorbic acid and the bismuth tree was 71.7% and 69.4% respectively at 1000 μg/mL as shown in FIG. 3. The activity inhibitory activity was confirmed to be similar to that of the positive control, ascorbic acid. In addition, as shown in Fig. 4, ascorbic acid and bisulfuric acid methanol extract exhibited 45.6% and 61.2% of elastase activity inhibition at 1000 μg/mL, respectively, so that the inhibitory effect of elastase activity was positive for the bisulfuric acid methanol extract. It was found to be superior to the control ascorbic acid.

In addition, according to another embodiment of the present invention, in order to confirm the whitening effect of the bisul tree extract, as a result of confirming the tyrosinase activity inhibitory effect by treating the bisul tree methanol extract with tyrosinase that induces pigmentation, FIG. 5 As shown, the inhibitory activity of tyrosinase activity of the positive controls arbutin and the methanol extract of Bisulaceae was 70.8% and 65.9%, respectively, at a concentration of 1000 μg/mL. It was confirmed at a level similar to that of.

From the above results, it was confirmed that the methanol extract of bisul tree has high potential for use as a functional natural material for skin whitening.

The cosmetic composition may be contained in an amount of 0.1 to 90 parts by weight of a bisul tree extract based on a total of 100 parts by weight of the composition.

The cosmetic composition may have a formulation of a flexible lotion, a nutritional lotion, a nutritional cream, an essence, or a pack.

The cosmetic composition may include a stabilizer, solubilizing agent, vitamins, pigments, and conventional adjuvants, such as a flavoring agent, and a carrier in addition to the extract of the bisul tree as an active ingredient.

The cosmetic composition may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, oil, powder foundation, emulsion foundation, It may be formulated as a wax foundation or spray, but is not limited thereto. More specifically, it may be prepared in the form of a sun cream, a flexible lotion, a converging lotion, a nutrition lotion, a nutrition cream, a massage cream, an essence, an eye cream, a pack, a spray, or a powder.

When the formulation is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicon, bentonite, silica, talc, or zinc oxide may be used as a carrier component. .

When the formulation is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane/butane Or a propellant such as dimethyl ether.

When the formulation is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3 -Butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the above formulation is a suspension, a liquid diluent such as water, ethanol or propylene glycol as a carrier component, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose , Aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

The present invention can provide a health food for skin whitening containing a bisul tree extract as an active ingredient.

The health food is used together with other foods or food additives in addition to the bismuth extract, and may be appropriately used according to a conventional method. The mixed amount of the active ingredient is suitable for its purpose of use, such as prevention, health, or therapeutic treatment. Can be determined.

The effective dose of the compound contained in the health food may be used in accordance with the effective dose of the therapeutic agent, but in the case of long-term intake for the purpose of health and hygiene or health control, it may be less than the above range, and is effective. It is clear that the ingredient can be used in an amount beyond the above range because there is no problem in terms of safety.

There is no particular limitation on the kind of health food, for example, meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, Drinks, alcoholic beverages, and vitamin complexes.

In addition, the present invention may be provided as a reagent composition for inhibiting the activity of tyrosinase, collagenase, or elastase containing the bisul tree extract as an active ingredient.

Hereinafter, examples will be described in detail to aid understanding of the present invention. However, the following examples are for illustrative purposes only, and the scope of the present invention is not limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

<Reference Example> Reagents and equipment

Folin &Ciocalteu's phenol reagent, DMSO (dimethyl sulfoxide), diethylene glycol, potassium peroxodisulfate, 1,1-diphenyl-2-polylhydrazyl (1,1-diphenyl-2- polryl hydrazyl; DPPH), tannic acid, rutin, 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg, N -succinyl-(L-Ala) ₃ -p -(nitroanilide), Collagenase (from Clostridium histolyticum) , elastase (from porcine pancreas), tyrosine, and tyrosinase (from mushroom) were manufactured by Sigma (St. Louis, MO, USA), and others. The general reagents used the special products.

<Example 1> Preparation of bisul tree extract

The methanol extract of Ulmus pumila L. was sold and used by the Korea Plant Extract Bank. The trunk of the secret liquor tree was collected in September 2001 in Yeongwol, Gangwon-do. 100 g of the pulverized sample and 1 L of 100% methanol were added to the flask, followed by extraction at 45° C. for 1 hour, which was repeatedly extracted three times. The extract was filtered, concentrated and dried.

The yield of the methanol extract of Bismuth tree was 4.5%, and the sample was dissolved in DMSO before use.

<Example 2> Confirmation of antioxidant activity

2-1. Determination of total polyphenol and total flavonoid content

The total polyphenol content was confirmed by the Folin-Denis method (Folin & Denis, 1912). 1 mL of folin-reagent was added to 1 mL of the sample diluted to a concentration of 200 μg/mL, reacted at room temperature for 3 minutes, and then 1 mL of 10% Na2CO3 was mixed and left at room temperature for 1 hour to measure absorbance at 725 nm. The calibration curve was prepared using tannic acid.

The total flavonoid content was confirmed by a variant of Davies et al. (1980). 1 mL of diethylene glycol was mixed with 100 μL of the sample diluted to 200 μg/mL, reacted at room temperature for 5 minutes, and then 100 μL of 1 N NaOH solution was added and left at 37°C for 1 hour, and the absorbance was measured at 420 nm. , The calibration curve was prepared using a routine.

As a result, as shown in FIG. 1, the total polyphenol and total flavonoid contents of the methanol extract of Bisul tree were 418.9 TAE (tannic acid equivalent) mg/g and 236.1 RE (rutin equivalent) mg/g, respectively.

2-2. DPPH radical scavenging activity confirmation

The electron donating ability was confirmed by the method of Perez et al. (2004). To a 96-well plate, 50 μL of 0.2 mM DPPH solution dissolved in ethanol and 100 μL of methanol extract of Bisulnamu were added at concentrations of 62.5, 125, 250, 500, and 1000 μg/mL, respectively, and allowed to stand for 30 minutes in a light-shielding state and then 517 nm. Absorbance was measured at. GraphPrism software Ver. 7 (Graphpad Software, lnc., La Jolla, CA, USA) was used to calculate SC ₅₀ (50% scavenging concentration, 50% free radical scavenging concentration).

As a result, as shown in Fig. 2, the methanol extract of Bisul tree showed a positive (+) dose-response correlation in a concentration-dependent manner, and the electron donating capacity of the positive control group, ascorbic acid and the methanol extract of Bisul tree, was at 1000 μg/mL, respectively. It was found to be 97.7% and 90.0%, and the SC ₅₀ of the methanol extract of the bismuth was found to be 282.9 μg/mL, whereas the SC ₅₀ of ascorbic acid was not calculated, but it was found to be smaller than 62.5 μg/mL.

<Example 3> Enzyme activity confirmation

3-1. Confirmation of collagenase activity inhibitory ability

4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly- in 0.1 M Tris-HCl buffer (pH 7.5) containing 100 μL of the sample diluted to 62.5, 125, 250, 500 and 1000 μg/mL and 4 mM CaCl ₂ After mixing 250 μL of a substrate solution in which Pro-D-Arg (0.3 mg/mL) was dissolved, 150 μL of collagenase (0.2 mg/mL) was added and reacted at room temperature for 20 minutes.

Thereafter, 1 mL of 6% citric acid was mixed, and 1.5 mL of ethyl acetate was mixed to stop the reaction. Then, the supernatant was taken and the absorbance was measured at 320 nm. Ascorbic acid was used as a positive control, and IC ₅₀ (50% inhibitory concentration, concentration that inhibits collagenase activity to 50%) was calculated using GraphPrism software.

As a result, as shown in FIG. 3, the methanol extract of Bisul tree showed a positive (+) dose-response correlation in a concentration-dependent manner, and the inhibitory activity of collagenase activity of the positive control, ascorbic acid and methanol extract, was 1000 μg/ In mL, they were 71.7% and 69.4%, respectively. In addition, the IC ₅₀ of the methanol extract of ascorbic acid and bisulnamu was found to be 360.3 μg/mL and 320.9 μg/mL, respectively.

3-2. Confirmation of inhibitory ability of elastase activity

5 μL of 0.2 unit of porcine pancrease elastase dissolved in 15 μL of samples diluted to 62.5, 125, 250, 500 and 1000 μg/mL in 100 mM Tris-HCl buffer (pH 8) and N- 20 μL of a substrate solution in which succcinyl-(L-Ala)3-p-nitroa-nilide (0.5 mg/mL) was dissolved was added and reacted at 37° C. for 10 minutes.

After cooling for 5 minutes on ice, the absorbance was measured at 410 nm. Ascorbic acid was used as a positive control, and IC ₅₀ (50% inhibitory concentration, concentration inhibiting elastase activity to 50%) was calculated using GraphPrism software.

As a result, as shown in FIG. 4, the methanol extract of Bisul tree showed a positive (+) dose-response correlation in a concentration-dependent manner, and the inhibitory activity of elastase activity of the methanol extract of ascorbic acid and Bisul tree, which was a positive control, was 1000 μg/ It was found to be 45.6% and 61.2% in mL, respectively, and the IC ₅₀ of the methanol extract of Bisulwood was found to be 731.1 μg/mL.

On the other hand, the IC ₅₀ of ascorbic acid was not calculated, but it was found to be greater than 1000 μg/mL.

3-3. Confirmation of tyrosinase (Mushroom tyrosinase) activity inhibition

Add 50 μL of sodium phosphate buffer (pH 6.8), 50 μL of 0.03% tyrosine, and 10 μL of mushroom tyrosinase to 5 μL of the sample diluted to 62.5, 125, 250, 500 and 1000 μg/mL. After reacting at °C for 10 minutes, it was cooled on ice for 5 minutes, and absorbance was measured at 475 nm. Arbutin was used as a positive control, and IC ₅₀ (50% inhibitory concentration, concentration that inhibits tyrosinase activity to 50%) was calculated using GraphPrism software.

As a result, as shown in FIG. 5, the methanol extract of Bisul tree showed a positive (+) dose-response correlation in a concentration-dependent manner, and the inhibitory activity of mushroom tyrosinase activity between the positive control arbutin and the methanol extract of Bisul tree was 1000 μg/ The concentrations in mL were 70.8% and 65.9%, respectively, and the IC _50s of the methanol extract of arbutin and bisulnamu were found to be 240.7 μg/mL and 258.6 μg/mL, respectively.

As described above, specific parts of the present invention have been described in detail, and for those of ordinary skill in the art, it is obvious that these specific techniques are only preferred embodiments, and the scope of the present invention is not limited thereby. something to do. Therefore, it will be said that the practical scope of the present invention is defined by the appended claims and their equivalents.

Claims (6)

A cosmetic composition for skin whitening containing a bisul tree extract as an active ingredient. The cosmetic composition for skin whitening according to claim 1, wherein the bismuth extract is extracted with water, C1 to C4 alcohol, or a mixed solvent thereof. The cosmetic composition for skin whitening according to claim 1, wherein the bisul tree extract inhibits the activity of tyrosinase, collagenase, or elastase. The cosmetic composition for skin whitening according to claim 1, wherein the cosmetic composition contains 0.1 to 90 parts by weight of a bisul tree extract based on a total of 100 parts by weight of the composition. The method of claim 1, wherein the cosmetic composition is a cosmetic composition for skin whitening, characterized in that it has a formulation of a softening lotion, a nutrient lotion, a nutritional cream, an essence or a pack. A health food for skin whitening that contains bisul tree extract as an active ingredient.

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