KR20200104159A - Cosmetic composition comprising saururus chinensis extracts cultivated by aquaponics - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a Saururus chinensis extract cultivated by aquaponics. The cosmetic composition may exhibit antioxidant, whitening or anti-inflammatory effects.

Description

Cosmetic composition containing extract of Sambaekcho using Aquaphonics cultivation technology {COSMETIC COMPOSITION COMPRISING SAURURUS CHINENSIS EXTRACTS CULTIVATED BY AQUAPONICS}

The present invention relates to a cosmetic composition comprising an extract of Sambaekcho grown through aquaponics cultivation. The cosmetic composition may exhibit antioxidant, whitening or anti-inflammatory effects.

The skin is a single membrane covering the outside of the body and plays a role in protecting and regulating internal organs and other internal organs by performing various physiological functions that protect the body from environmental factors such as various external stimuli, obstacles, and dryness. do.

However, with age, secretion of various hormones that regulate metabolism internally decreases, and functions of immune cells and activity of cells decrease. In addition, externally, the content of ultraviolet rays in the sun's rays increases due to the destruction of the ozone layer, and excessive physical and chemical irritation and stress caused by the increase of free radicals and active harmful oxygen, etc., as the environmental pollution increases It damages the skin by promoting deterioration, the generation of spots and freckles due to melanin deposition, and skin aging.

In order to prevent skin aging and damage caused by such internal and external factors to maintain healthier and more beautiful skin, physiologically active substances obtained from various animals, plants, microorganisms, etc. are added to cosmetics to maintain the skin's own function and Efforts to maintain skin health by activating cells and promoting skin metabolism are continuing, and cosmetics and external preparations for skin are being developed accordingly.

However, when these substances are applied to the skin, the amount of use of these substances is limited due to safety problems such as irritation, erythema, and redness, or the effect is insignificant, making it difficult to substantially improve skin function. Therefore, there is a need to develop a new composition for external application for skin that is safer to the living body and has higher effect than the existing composition for external application for skin.

Korean Patent Registration No. 10-1370075 Korean Patent Registration No. 10-1551748

It is an object of the present invention to provide a cosmetic composition for antioxidants comprising an extract of Saururus chinensis obtained through the cultivation of aqua phonics.

One aspect of the present invention for achieving the above object relates to an antioxidant cosmetic composition comprising an extract of Saururus chinensis obtained through cultivation of aqua phonics.

In the present invention, " Saururus chinensis " is a perennial plant of a plant of the family Sambaek and is widely distributed in China, Japan and Korea. In oriental medicine, Sambaekcho is known to be effective in dropsy, jaundice, adult diseases, fever, diuresis, and liver cancer. Tetrahydrofurans, a lignan derivative isolated from Sambaekcho, have been reported to inhibit cell adhesion, protect liver, and inhibit eating.

In the present invention, "aquaphonics" is a combination of aquaculture meaning aquaculture and hydroponics meaning hydroponic cultivation of plants, and uses the excrement of fish as nutrients for plants, , It is a method of artificial plant cultivation that uses the water naturally purified by the biological action of plants for fish farming. Unlike humans or other animals, fish excrement is mainly composed of ammonia or ammonium, and ammonia due to the decomposition of bacteria while passing the growth water of such fish excrement, ammonia or ammonium-containing fish through a separate bacterial culture tank Or converting ammonium to nitrate. The growth water of fish containing nitrate converted as described above is supplied to the crop, and the crop is grown by ingesting nitrate in the growing water as a nutrient, and the remaining growth water is returned to the fish again.

Specifically, the three baekcho obtained through the aquaponics cultivation may be grown at 24 ~ 27 ℃, can be cultivated for 6 to 9 months. In one embodiment of the present invention, extracts were prepared by obtaining Sambaekcho grown at the temperature and period, and it was confirmed that the effect of the extract exhibited remarkably superior antioxidant, whitening or anti-inflammatory effects compared to the extract of Sambaekcho harvested by a general cultivation method. I did.

The "extract" of the present invention is an extract obtained by extraction treatment of 300 seconds, a diluted solution or concentrate of the extracts, a dried product obtained by drying the extract, a preparation or purified product of the extract, or a mixture thereof, the extract itself and the extract It includes extracts of all formulations that can be formed using.

The extract of the present invention may be extracted from natural, hybrid, or variant plants of the Sambaekcho, and may be extracted from plant tissue culture.

The method of extracting the extract of the present invention is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include a hot water extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like, which may be performed alone or in combination of two or more methods.

The type of the extraction solvent used in the present invention is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water, C ₁ to C ₄ anhydrous or lower alcohol, a mixed solvent of water and lower alcohol, acetone, 1,3-butylene glycol, ethyl acetate, selected from the group consisting of chloroform It can be used alone or in combination of two or more.

More specifically, water (or distilled water) may be used as the extraction solvent in the present invention. The solvent extract can be prepared by extracting the three hundred seconds more than once using the solvent, and a dry extract obtained by distilling the solvent extract under reduced pressure and then freeze-drying or spray drying can be prepared. In a specific embodiment of the present invention, a Sambaekcho extract obtained by cultivation of aqua phonics was prepared using water (or distilled water) as a solvent (Example 1).

In the present invention, the extract obtained by heat extraction or cold needle extraction is filtered to remove suspended solid particles, for example, nylon, etc. to filter the particles, or after filtration using a freeze filtration method, etc., and then used as such, or freeze-dried, hot-air drying, It can be used after drying by spray drying or the like.

In the present invention, “antioxidation” refers to an action of inhibiting oxidation, and in the human body, an oxidation promoting substance and an oxidation inhibitor are in balance, but due to various factors, the balance is lost and the direction of promoting oxidation When inclined toward, oxidative stress is induced in a living body, leading to cell damage and pathological diseases. Reactive oxygen species (ROS), which are the direct cause of oxidative stress, are chemically unstable and highly reactive, so they can easily react with various biomaterials such as DNA, proteins, lipids, and carbohydrates. Attacks cause irreversible damage to cells and tissues, or cause mutations, cytotoxicity and cancer, and are also a direct cause of aging. By removing or reducing these reactive oxygen species, antioxidant effects are obtained, preventing aging and maintaining health.

In one embodiment of the present invention, the antioxidant effect was confirmed by measuring free radical scavenging activity, and in particular, the Sambaekcho extract obtained through the aqua phonics cultivation method showed superior antioxidant effect compared to the Sambaekcho extract through general cultivation. Confirmed (Table 1 and Table 2).

Specifically, the cosmetic composition of the present invention may further include whitening or anti-inflammatory use.

In the present invention, "whitening" means to whiten the skin, and it refers to alleviating or improving various pigmentation such as spots and freckles caused by excessive synthesis of melanin, activity of tyrosinase enzyme, and production of melanin, and , Skin tone or complexion may be improved through the whitening effect.

In the present invention, “skin complexion” and “skin tone” refer to the color or tone expressed by the skin, and if the complexion is dark or the skin tone is dull, the satisfaction with makeup may be lowered. “Improving skin complexion” and “Improving skin tone” include all cases in which the skin brightness is brightened by increasing the brightness level of the skin to increase the satisfaction with makeup, or a brighter skin tone can be obtained even without makeup.

In one embodiment of the present invention, when the extract of Sambaekcho obtained through cultivation of aquaponics or a cosmetic composition containing the extract is applied, excellent tyrosinase inhibitory activity (Table 3) and melanin biosynthesis inhibitory activity (Table 4) are shown. As a result, it was confirmed that it can exhibit substantially remarkably excellent whitening effect or skin complexion improvement effect, and it was confirmed that it can be used for whitening purposes, overall skin complexion and skin tone improvement purposes.

In the present invention, the term "anti-inflammatory" refers to suppressing inflammation, and the inflammation is one of the defense responses of living tissues against certain stimuli, and refers to a complex lesion that causes three types of tissue deterioration, circulatory disorders and effusion, and tissue proliferation. .

Inflammatory reaction is a mechanism to regenerate damage caused by physical action, harmful substances, chemical stimulation, bacterial infection, etc. as part of the defense response of living tissue against external stimuli. The persistent inflammatory reaction rather causes damage to the mucous membrane and promotes damage, inflammation and skin troubles in the skin tissue.

Macrophages play a very important role in the immune response and regulate a variety of inflammatory mediators, including nitric oxide, prostaglandins, and inflammatory cytokines, an immune protein. Among them, nitric oxide plays an important role in killing bacteria or removing tumors, but excessive nitric oxide production by pathogenesis causes inflammation, and such inflammation causes skin trouble.

That is, when the production of nitric oxide by macrophages is suppressed, skin inflammation can be prevented, alleviated, improved, and treated, and skin problems caused by inflammatory reactions can be prevented, alleviated, improved, and treated.

In an embodiment of the present invention, it was confirmed that the production of nitrogen monoxide was significantly reduced compared to the Sambaekcho extract obtained through general cultivation when the extract of Sambaekcho obtained through aquaponics cultivation was treated (Table 5), and TNF-, an inflammatory mediator It was confirmed that the inhibitory activity of α, IL-6 and IL-1ß production was also remarkably excellent when treated with the extract of Sambaekcho obtained through cultivation of Aquaphonics (Table 6).

The cosmetic composition of the present invention is a solution, external ointment, cream, foam, nutrient lotion, flexible lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen cream, sun oil, suspension, emulsion It can be prepared in a formulation selected from the group consisting of liquid, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch, and spray, but is limited thereto. no.

The cosmetic composition of the present invention may additionally include one or more cosmetically acceptable carriers blended in general skin cosmetics, and as common ingredients, for example, oil, water, surfactants, moisturizers, lower alcohols, thickeners , A chelating agent, a colorant, a preservative, a fragrance, and the like may be appropriately blended, but are not limited thereto.

The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohard It may include a propellant such as locarbon, propane/butane or dimethyl ether, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, and the like may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, micro Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used, but are not limited thereto. These may be used alone or in combination of two or more.

Specifically, in the cosmetic composition as described above, the Sambaekcho extract may be included in an amount of 0.0001% to 30.0% by weight based on the total weight of the cosmetic composition. More specifically, it may be included in 0.005% to 20% by weight. There is an advantage of exhibiting excellent antioxidant, whitening or anti-inflammatory effects within the above range, and there is an advantage that the formulation of the composition is stabilized.

The cosmetic composition comprising the extract of Sambaekcho obtained through cultivation of aqua phonics of the present invention has excellent antioxidant, whitening or anti-inflammatory effects.

In addition, since the composition of the present invention is a material derived from a natural product, it is not toxic, and even when applied to the human body, it does not cause side effects, so its utilization as a cosmetic composition is high. In particular, all raw materials used in the present invention correspond to the first grade on the EWG (Environmental Working Group) grade, and have proven excellent safety.

The effects of the present invention are not limited to the above effects, and should be understood to include all effects that can be deduced from the configuration of the invention described in the detailed description or claims of the present invention.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are only illustrative of the present invention, and the present invention is not limited by the following examples.

Example 1. Preparation of Sambaekcho extract (Aquaponics Sambaekcho extract) grown through Aquaphonics cultivation

1-1. Cultivation of Sambaekcho using aqua phonics cultivation method

Sambaekcho was cultivated using an aquaponics system including a fish farming tank. Specifically, a system was used so that nitrate generated while growing fish could be supplied to 300 seconds, and the growth water containing ammonia was converted into nitrate by the action of bacteria while passing through the bacteria culture tank. After cultivation for 6 to 9 months at 24 ~ 27 ℃ using the above aqua phonics cultivation was harvested 300 seconds.

1-2. Preparation of Sambaekcho extract

100 g of the dried weight of the harvested three hundred seconds was put into a flask and extracted by cold soaking with 1,000 g of an extraction solvent (distilled water) for 3 days. The chilled extract was filtered through a filter having a pore size of 0.2 μm to prepare a Sambaekcho extract.

Comparative Example 1. Preparation of extracts of Sambaekcho grown through general cultivation (general Sambaekcho extract)

After cultivating Sambaekcho using a cultivation method commonly used in the art, it was harvested. Thereafter, an extract of Sambaekcho obtained by general cultivation was prepared using the same extraction method as in 1-2 above.

Experimental Example 1. Antioxidant effect confirmation

1-1. DPPH scavenging activity confirmation

The antioxidant activity of Example 1 and Comparative Example 1 was confirmed. For the evaluation of antioxidant activity, DPPH (1,1-diphenyl1-2-picry1hydrazy1) was used to measure the free radical scavenging activity. Specifically, after adding 0.6 mL of the extract of Example 1 and Comparative Example 1 to 2.4 mL of 4.0 x 10 ^-4 M DPPH solution, each of the extracts of Example 1 and Comparative Example 1 was added, shaken for 10 seconds with a vortex mixer, left at room temperature for 30 minutes, and then measured the absorbance at 517 nm to control the Antioxidant activity was shown as a reduction ratio of absorbance to At this time, as a control for determining the rate of decrease in absorbance, ascorbic acid, an antioxidant, was used, and the same amount as the sample was added and measured in the same manner. Then, DPPH scavenging activity was calculated according to Equation 1 below.

[Equation 1]

DPPH scavenging activity (%) = [1-(absorbance of sample treatment group in Example or Comparative Example/absorbance of sample untreated group)] X 100

sample DPPH elimination (%) Comparison of antioxidant activity (%) Ascorbic acid 31 100 Example 1 87 280.6 Comparative Example 1 74 238

As shown in Table 1, it was found that both Example 1 and Comparative Example 1 exhibited DPPH scavenging activity. However, in the case of Example 1 obtained through cultivation of aquaponics, it was confirmed that it exhibits excellent antioxidant effect compared to Comparative Example 1.

1-2. ABTS ⁺ Confirmation of radical scavenging activity

ABTS ⁺ [2,2'-Azino-bis(3-ethyl benzothiazoline-6-sulfonic acid)] radical scavenging activity was measured for both hydrogen-donating antioxidants and chainbreaking antioxidants. It is a measurement method applicable to both the aqueous phase and the organic phase.

The 7 mM ABTS solution was mixed so that potassium persulfate became 2.45 mM, reacted in the dark for about 24 hours, and ABTS ⁺ solution was mixed with the sample, reacted for 6 minutes in the dark, and the absorbance was measured at 734 nm. The resulting value was expressed as a percentage (%) of the radical scavenging activity through calculation using Equation 1 above by comparing the extract sample-treated group and the untreated group, and BHT was used as a positive control group (Table 2).

sample ABTS ⁺ Elimination (%) Comparison of antioxidant activity (%) BHT 13 100 Example 1 33 253 Comparative Example 1 21 161

As shown in Table 2, it was found that both Example 1 and Comparative Example 1 exhibited ABTS ⁺ radical scavenging activity. However, in the case of Example 1 obtained through cultivation of aquaponics, it was confirmed that it exhibits excellent antioxidant effect compared to Comparative Example 1.

Experimental Example 2. Whitening improvement effect confirmation

2-1. Confirmation of tyrosinase inhibitory activity

In order to confirm the whitening improvement effect of the extract of Sambaekcho obtained through cultivation of aqua phonics, the inhibitory activity of tyrosinase used as an enzyme to form melanin was confirmed.

Specifically, 110 µl of 0.1 M phosphate buffer (pH 6.5) was added to a corning flat 96 well plate, followed by 10 µl of 250 mM tyrosinase, and 30 µl of each of Example 1 and Comparative Example 1 samples. After addition, 20 µl of 1.5mM L-tyrosine as a substrate was added and reacted for 20 minutes in an incubator at 37°C. Then, the absorbance was measured at 490 nm using a spectrometer (Fluorescent microplate reader). Arbutin (Sigma, USA) was dissolved in purified water and used as a positive control. The tyrosinase inhibitory activity was calculated using the following formula, and the results are shown in Table 3.

[Equation 2]

Tyrosinase inhibitory activity (%) = [1-(absorbance of Example or Comparative Example sample treated group/absorbance of sample untreated group)] X 100

As shown in Table 3, when applied to Example 1, it was confirmed to exhibit remarkably superior tyrosinase inhibitory activity compared to Comparative Example 1 or the positive control. In particular, it was confirmed that the tyrosinase inhibitory activity of Example 1 was high overall at the treatment concentration of 50 to 500 ug/ml, and the whitening effect of the extract of Sambaekcho obtained through Aquaphonics cultivation was excellent.

2-2. Confirmation of melanin biosynthesis inhibitory activity

To measure the inhibition of melanin biosynthesis, melanoma cells cultured in DMEM medium were dispensed to a 100mm culture dish at a concentration of 2×10 ⁶ cells/dish, and after culturing for 24 hours, 2 mL of samples of Example 1 and Comparative Example 1 were added for each concentration. After 48 hours, it was washed with phosphate buffer (pH 7.4). Then, after detaching the cells with 0.25M trypsin EDTA solution, the harvested cells were treated with 1 mL of 5% TCA per 1×10 ⁶ cells, centrifuged twice at 2,500 rpm, and the separated melanin was washed with phosphate buffer. Then, 1 mL of ether: ethanol (1:3) was added, centrifuged twice, and washed and dried with 1 mL of ether. 1 mL of 1N NaOH was added to the dried melanin, reacted at 80° C. for 1 hour, and absorbance was measured at 405 nm with a spectrophotometer. Inhibition of melanin biosynthesis was measured using the absorbance of the sample-treated group and the untreated group using Equation 3 below, and the results are shown in Table 4. Arbutin (Sigma, USA) was dissolved in purified water and used as a positive control.

[Equation 3]

Melanin biosynthesis inhibitory activity (%) = [1-(absorbance of Example or Comparative Example sample treated group/absorbance of sample untreated group)] X 100

As shown in Table 4, it was confirmed that when Example 1 was applied, it exhibited remarkably superior melanin biosynthesis inhibitory activity compared to Comparative Example 1 or the positive control. In particular, it was confirmed that the melanin biosynthesis inhibitory activity of Example 1 was generally high in the entire treatment concentration of 50 to 500 ug/ml, and the whitening effect of the extract of Sambaekcho obtained through Aquaphonics cultivation was excellent.

Experimental Example 3. Evaluation of anti-inflammatory activity

When the RAW 264.7 cells were stimulated with LPS (LPS(+)) and not (LPS(-)) as a control, the anti-inflammatory effect was confirmed.

3-1. Confirmation of inhibition of nitrogen monoxide (NO) production

For the measurement of NO production, the concentration of nitrite in the cell culture medium was measured using a Grease reagent. RAW 264.7 cells were dispensed into a 12 well plate so that the number of cells was 1 × 10 ⁵ cells/well, and cultured with 80% confluence. Then, each of the extracts of Example 1 and Comparative Example 1 was treated, and NO production was induced with LPS (100 ng/mL) for 24 hours, and then the culture supernatant and the grease reagent were mixed at 1:1 using an ELISA reader. NO production was measured at 540 nm. After making a standard curve using Sodium Nitrite (NaNO ₂ ), it was used for NO measurement. The amount of NO produced as a percentage is shown in Table 5 below.

As shown in Table 5, it was confirmed that when Example 1 was treated, the amount of reduction in NO generation was increased compared to when Comparative Example 1 was treated. From this, it was confirmed that the extract of Sambaekcho obtained through the cultivation of Aquaphonics has excellent anti-inflammatory activity.

3-2. Measurement of cytokines in cell culture

Examples and Comparative Example 1 The inhibitory activity of cytokines, an inflammatory mediator, according to the treatment of the extract was evaluated. Specifically, after adjusting RAW 264.7 cells to a concentration of 1×10 ⁶ cells/mL, 200 μL each was dispensed into a 96 well plate, stabilized in an incubator, and after 12 hours, the culture medium was removed and a new medium 160 μL After dispensing, 20 μL of each of the samples of Example 1 and Comparative Example 1 at a concentration of 5 μg/mL was added and reacted for 30 minutes. As a negative control, distilled water was treated instead of the sample.

Thereafter, 20 μL of LPS at a concentration of 1 μg/mL was treated and rearranged for 24 hours, and tumor necrosis factor-alpha (TNF-α), interleukin-1ß (IL-1ß and interleukin-6 (IL-6) released from the supernatant were ) Was measured using ELISA method (BD Bioscience, East Rutherford, NJ, USA) or Griess reagent In the case of the positive control, the sample was not treated and only LPS was treated. And the production amount of IL-1ß is shown in Table 6 below as a percentage.

As shown in Table 6, when Example 1 was treated, it was confirmed that the inhibitory effect of TNF-α, IL-6, and IL-1ß production was excellent compared to the case of Comparative Example 1, and from this, aquaponics cultivation It was confirmed that the anti-inflammatory activity of the extract obtained through Sambaekcho was excellent.

Preparation Example 1. Preparation of Essence Containing the Extract of Aquaponics Cultivated Sambaekcho of the Invention

A cosmetic composition of an essence formulation was prepared as shown in Table 7 below (unit: wt%).

Preparation Example 2. Preparation of a cream containing the mixture of the present invention

As shown in Table 8 below, a cream-formed cosmetic composition was prepared (unit: wt%).

The above description of the present invention is for illustrative purposes only, and those of ordinary skill in the art to which the present invention pertains can understand that it is possible to easily transform it into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as being distributed may also be implemented in a combined form.

The scope of the present invention is indicated by the claims to be described later, and all changes or modified forms derived from the meaning and scope of the claims and the concept of equivalents thereof should be interpreted as being included in the scope of the present invention.

The present invention is the result of the 2018 Jeju Advanced Science and Technology Complex BT Company's raw material crop cultivation research support project supported by the Jeju Free International City Development Center.

Claims (6)

A cosmetic composition for antioxidant comprising an extract of Saururus chinensis grown through aqua phonics cultivation. The method of claim 1,
The three hundred seconds will be grown at 24 ~ 27 ℃, antioxidant cosmetic composition. The method of claim 1,
The three hundred seconds will be grown for 6 to 9 months, antioxidant cosmetic composition. The method of claim 1,
The extract is one or more solvents selected from the group consisting of water, C ₁ to C ₄ anhydrous or lower alcohol, a mixed solvent of water and lower alcohol, acetone, 1,3-butylene glycol, ethyl acetate, and chloroform. Cosmetic composition obtained by extraction. The cosmetic composition of claim 1, wherein the extract of Sambaekcho is contained in an amount of 0.0001 to 30% by weight based on the total weight of the composition. The method according to any one of claims 1 to 5,
The cosmetic composition will further include a whitening or anti-inflammatory use, cosmetic composition.