KR20200083355A - A method for cultivating ginseng sprout - Google Patents

Abstract

The present specification discloses a method of cultivating sprout ginseng. The method includes a step of culturing after planting a raw material for cultivating sprout ginseng in a culture soil in a plant factory, and the plant factory has an effect of increasing a saponin content as well as preventing soft rot or fungal contamination of sprout ginseng by including a wall part made of loess.

Description

How to grow sprout ginseng{A METHOD FOR CULTIVATING GINSENG SPROUT}

Disclosed herein is a method of cultivating sprout ginseng.

Ginseng is categorized into cultivated artificially grown ginseng (commonly called ginseng) in ginseng fields, ginseng ginseng (wild ginseng), a ginseng grown in nature like wild ginseng by artificially spraying ginseng seeds in deep mountains. Can.

Cultivated ginseng began to grow by hand by sowing seeds of wild ginseng collected from nature in the late Joseon Dynasty, and today ginseng is often referred to as cultivated ginseng. Wild ginseng, wild ginseng, grew wild throughout the country, and its number began to decrease rapidly from the end of Goryeo. When it became difficult to obtain wild ginseng and its cost became high, some people began to cultivate wild ginseng in deep mountains, thereby cultivating wild ginseng (Camphor ginseng) and general cultivated ginseng.

On the other hand, goats over 20 years old have problems containing residual pesticides and heavy metals, and cannot be produced or distributed in winter.

KR 10-1319082 B1

In one aspect, the present specification aims to provide a method for growing ginseng sprouts.

In another aspect, the present specification aims to provide a method for producing a large amount of compound K from sprout ginseng through physical processing and enzymatic processing.

In one aspect, the technology disclosed herein includes a culture step of cultivating a raw ginseng cultivation material on a culture soil in a plant factory, and then culturing the plant factory, wherein the plant factory provides a method for cultivating ginseng sprouts comprising a wall made of yellow soil.

In one exemplary embodiment, the upper portion of the plant factory may be made of ocher.

In one exemplary embodiment, the wall portion may be made of 50 to 150 cm thick ocher.

In one exemplary embodiment, the cultivation method may be to supply water to the loess to control the temperature in the plant factory to 13 to 23 °C.

In one exemplary embodiment, the culturing step may include irradiating blue LED light.

In an exemplary embodiment, the culturing step may include supplying a nutrient solution containing wasabi ( Eutrema japonicum (Miq.) Koidz) fermentation and deep sea water.

In one exemplary embodiment, the horseradish fermentation product and deep sea water may be mixed in a volume ratio of 1:5 to 1:100.

In one exemplary embodiment, the horseradish fermentation product may be fermented leaves and roots of horseradish.

In an exemplary embodiment, the horseradish fermentation product may be fermented by mixing wasabi, water, and sugar.

In one exemplary embodiment, the culture step may be carried out for 3 to 6 weeks.

In another aspect, the techniques disclosed herein provide sprout ginseng grown according to the above method.

In one exemplary embodiment, the cultivated sprout ginseng may contain a compound K of 1,000 ug / g or more.

In one aspect, the technology disclosed herein has the effect of providing a method for growing ginseng sprouts.

In another aspect, the technology disclosed herein has the effect of providing a method for producing a large amount of compound K from bud ginseng through physical processing and enzymatic processing.

1 is a photograph showing the experiment of the BuOH fraction of Experimental Example 1 of the present specification.
Figure 2 shows the HPLC analysis method of Experimental Example 1 of the present specification.
Figure 3 shows the saponin standard.
4 to 6 show the results of saponin composition analysis for each extraction site of sprout ginseng.
7 shows a photograph of the experiment of BuOH fraction of Experimental Example 2 of the present specification.
Figure 8 shows the HPLC analysis method of Experimental Example 2 of the present specification.
9 and 10 show the results of Compound K analysis of sprout ginseng processed according to an experimental example of the present specification.

Hereinafter, the present invention will be described in detail.

In one aspect, the technology disclosed herein comprises a culture step of culturing the plant after cultivating the raw material of sprout ginseng on a culture soil in a plant factory, wherein the plant factory comprises a wall portion made of ocher, a method for growing sprout ginseng. to provide.

When growing ginseng in a conventional plant factory, problems such as moss disease and mold contamination occur, whereas the method for growing ginseng in accordance with the present specification contaminates an rot or fungus by growing sprout ginseng in a plant factory that includes a wall made of loess. In addition to preventing the effect of increasing the saponin content.

In one exemplary embodiment, the upper portion of the plant factory may be made of ocher.

The wall part means a part that structurally forms a wall, and may be formed of four wall surfaces or may surround a plant factory in a circular shape. Doors and/or windows that can be accessed may be formed in the wall portion.

The upper portion means a portion that structurally forms a ceiling, and if necessary, sunlight may be formed to be incident or may be partially or entirely made of ocher.

In an exemplary embodiment, when the wall portion surrounds the plant factory in a circular shape, the upper portion and the wall portion may be integrally formed without a boundary surface separating the upper portion from the wall portion.

In one exemplary embodiment, the wall portion may be made of 50 to 150 cm thick ocher. When the ocher thickness is 50 cm or less, the effect of preventing the growth of ginseng sprouts and mold contamination and the effect of enhancing the saponin content decreases, and when the ocher thickness exceeds 150 cm, there is no effectiveness due to poor economic efficiency.

In one exemplary embodiment, the cultivation method may be to supply water to the loess to control the temperature in the plant factory to 13 to 23 °C.

The method for cultivating sprout ginseng according to the present specification provides a thermal insulation effect including a wall portion made of yellow soil, and a low temperature control system through evaporation of water supplied to the soil can easily adjust the inside of a plant plant to a temperature suitable for growing sprout ginseng. For example, when the temperature inside the plant factory is high, water can be supplied to the loess to lower the temperature through evaporation of water. Accordingly, it is possible to easily cultivate ginseng sprouts in tropical regions or in areas where heat waves occur. In addition, the wall portion of the plant factory is made of red clay with a thickness of 50 to 150 cm, and the temperature does not drop significantly in winter, and the temperature can be easily adjusted to 13 to 23 °C, 13 to 20 °C, and 13 to 17 °C suitable for growing sprout ginseng. Through the heating and cooling effect as described above, it is possible to grow ginseng sprouts in all seasons regardless of the outside temperature.

In one exemplary embodiment, the water may be supplied by a method such as an injection method through a supply device such as a piping, a sprinkler, etc., and supply water to the inside or outside of the wall part (ie, the surface of the wall part) to plant plants I can control my temperature.

In one exemplary embodiment, the culturing step may include irradiating blue LED light.

In one exemplary embodiment, the culturing step comprises supplying a nutrient solution containing wasabi ( Eutrema japonicum (Miq.) Koidz) fermentation and deep sea water to prevent rot, fungal contamination, and increase the saponin content Giving effect.

In one exemplary embodiment, the horseradish fermentation product and the deep sea water may be mixed in a volume ratio of 1:5 to 1:100, a volume ratio of 1:10 to 1:100, or a volume ratio of 1:20 to 1:50. have.

In one exemplary embodiment, the horseradish fermentation product may be fermented leaves and roots of horseradish.

In an exemplary embodiment, the horseradish fermentation product may be fermented by mixing wasabi, water, and sugar. The saccharide means a compound that dissolves in water and has a sweet taste.

In an exemplary embodiment, the horseradish fermentation product may be fermented by further including deep ocean water.

In one exemplary embodiment, the saccharide may be molasses or sugar.

In one exemplary embodiment, the fermentation may be carried out at 30 to 40 ℃ or 30 to 35 ℃.

In one exemplary embodiment, the fermentation may be carried out for 25 to 100 hours, 50 to 75 hours, or 60 to 75 hours.

In one exemplary embodiment, the culture step may be carried out for 3 to 6 weeks. If the incubation period is 3 weeks or less, maturation of the above-ground portion may not be sufficient, and if the incubation period exceeds 6 weeks, the above-ground portion is overmatured, and the saponin content may decrease. Specifically, culturing for 4 to 5 weeks may be desirable in increasing the saponin content.

The method for cultivating sprout ginseng according to the present specification can produce sprout ginseng using not only ginseng for 1-2 years, but also wild ginseng for 20 years or more as a raw material for growing ginseng.

The method for cultivating sprout ginseng according to the present specification has an effect of removing residual pesticides and heavy metals contained in goat ginseng for more than 20 years by using wild ginseng as a raw material for growing sprout ginseng. In addition, it is possible to manufacture ginseng sprouts of wild ginseng in winter, and increase the compound K content of wild ginseng and produce leaves to increase product value.

In another aspect, the techniques disclosed herein provide sprout ginseng grown according to the above method.

In one exemplary embodiment, the sprout ginseng may be to increase the compound K content through steaming and enzyme fermentation process.

In one exemplary embodiment, the cultivated sprout ginseng compound K is 1,000 ug/g or more, 1,500 ug/g or more, 1700 ug/g or more, and more specifically 1,000 ug/g to 3,000 ug/g, 1,000 ug/ g to 2,500 ug/g or 1,000 ug/g to 2,000 ug/g.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as limited by these examples.

Example 1. sprout ginseng manufacturing

As a raw material for cultivation of sprout ginseng used in this example, ginseng ( Panax ginseng CA Meyer) was used as a one-year-old seedling harvested in Punggi, North Gyeongsang Province in October 2016.

For cultivation in a plant factory, the top box and masato were mixed in a weight ratio of 1:1 in a box of 64×23×16.6 cm. In one drawing box, 21 ginsengs (3 columns × 7 rows) were planted (7×9 cm planting distance). In a plant factory surrounded by walls and ceilings made of 60 to 80 cm thick ocher, the blue LED light is irradiated to the formal seedlings and contains fermented horseradish ( Eutrema japonicum (Miq.) Koidz) fermented water and deep sea water. Sprout ginseng was prepared for 6 weeks by supplying nutrient solution. The plant factory was able to supply water to the ocher wall inside the plant factory by spraying through a sprinkler. The temperature in the plant factory was maintained at 15±1℃ for both day and night, relative humidity was 60±5%, and CO ₂ concentration was supplied at 800 to 1,000 ppm.

The horseradish fermentation product, after crushing the leaves and roots of which the product value of horseradish was low, fill about 70% of the container with 100 L, add 30 L of deep ocean water, and fill the rest with water to make 100 L. Did. In addition, 500 g of molasses or sugar was added. Subsequently, the lid was closed to be sealed, and the temperature was maintained at about 30° C. or higher to ferment for 72 hours. The fermentation product after fermentation was completed was used by filtration. In this example, deep ocean water was obtained from Gangwon Deep Water Co., Ltd. (Ohori, Jukwang-myeon, Goseong-gun, Gangwon-do).

As a result of cultivation for 6 weeks, it was possible to cultivate ginseng sprouts with well-grown leaves without mold or scuffing.

Comparative example One.

In the same manner as in Example 1, the sprout ginseng was prepared, but was not a plant factory surrounded by walls and ceilings made of 60 to 80 cm thick ocher, but wasabi fermented water and deep sea water from a deeply-used common plant factory. Ginseng sprouts were prepared by supplying water instead of a nutrient solution containing.

As a result of cultivation for 6 weeks, mold developed and shriveled.

Experimental Example One.

The leaves, stems, and roots of sprout ginseng prepared in Example 1 and Comparative Example 1 were analyzed for irradiated saponin content and saponin composition, respectively.

The refrigerated sample was heated at 60°C with MeOH at a concentration of 70%, refluxed for 80 minutes, filtered and concentrated, dissolved in water, fractionated with BuOH three or more times, and concentrated to measure the irradiation saponin content. In the plant factory surrounded by walls and ceilings, it was confirmed that the sprout ginseng cultivated by supplying nutrient solution containing wasabi fermented water and deep sea water from the plant has higher irradiated saponin content in all areas than the normal sprout ginseng. Saponin composition analyzed according to the HPLC analysis method of FIG. 2 was found to be similar in bud ginseng of Example 1 and Comparative Example 1 (see FIGS. 3 to 6 and Table 1).

Sample volume menstruum
(70% MeOH) Irradiation content
(Example 1) Irradiation content
(Comparative Example 1) Major saponin composition leaf 5.9 g 177 mL 536.89mg 208.99mg F1, Rd, F2, Rg1+Re stem 10 g 200mL 155.28mg 73.34mg Rd, Rb1, Rg1+Re Root 15 g 225 mL 346.85mg 231.34mg Rb1, Rb2, Rd, Rg1+Re

Experimental Example 2.

The sprout ginseng prepared in Example 1 was steamed for 3 hours or more, and then enzyme (plantase AK, plantase UF + rohament CL) was added for fermentation for 24 hours, and the content of the compound K contained in the fermentation broth and sprout ginseng was analyzed. . When fermented with plantase AK enzyme, 60 g of sprout ginseng and 360 mL of water were mixed, and when fermented with plantase UF + rohament CL enzyme, 60 g of sprout ginseng and 480 mL of water were mixed. Compound K was analyzed by distilling and fermenting sprout ginseng with 70% MeOH at 60° C., refluxing for 80 minutes, filtration and concentration, dissolving in water, then fractionating and concentrating with BuOH. Specifically, the crude extract was dispersed in 30 ml of water and extracted three times with BuOH saturated with 20 ml of water, and then the butanol layer was concentrated to obtain an irradiated saponin layer. As a result of analyzing the compound K content according to the HPLC analysis method of FIG. 8, the cultivated and processed sprout ginseng was found to have a very high compound K content (see FIGS. 9 and 10, tables 2 and 3).

Standard concentration: 5mg/ml

Standard injection: 20uL

Sample concentration: 30mg/ml

Sample injection: 10uL

Com K Time Com K Area Com K 43.232min 1501.4 plantase AK 43.171min 269.8 plantase UF
rohament CL 43.135min 154

plantase AK plantase UF
rohament CL Com K content in fermentation broth 185ug/ml 217ug/ml Com K content in sprout 1110ug/g 1736ug/g

As described above, since a specific part of the present invention has been described in detail, for those skilled in the art, this specific technique is only a preferred embodiment, whereby the scope of the present invention is not limited. It will be obvious. Therefore, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (12)

Including the cultivation step of culturing the plant after cultivating the raw material for sprout ginseng on the culture soil in the plant factory,
The plant factory is to include a wall portion made of ocher, sprout ginseng cultivation method.
According to claim 1,
The upper part of the plant factory is made of yellow soil, sprout ginseng cultivation method.
According to claim 1,
Wherein the wall portion is made of 50 to 150 cm thick ocher, sprout ginseng cultivation method.
According to claim 1,
The cultivation method is to control the temperature in the plant factory by supplying water to the loess to 13 to 23 ℃, sprout ginseng cultivation method.
According to claim 1,
The culture step comprises irradiating blue LED light, sprout ginseng cultivation method.
According to claim 1,
The cultivation step comprises supplying a nutrient solution containing wasabi ( Eutrema japonicum (Miq.) Koidz) fermentation and deep sea water, sprout ginseng cultivation method.
The method of claim 6,
The horseradish fermentation product and the deep sea water are mixed in a volume ratio of 1: 5 to 1: 100, and the method of cultivating sprout ginseng.
The method of claim 6,
The horseradish fermentation product was fermented leaves and roots of horseradish, a method of cultivating sprout ginseng.
The method of claim 6,
The horseradish fermentation product was a fermented mixture of horseradish, water and sugar, and the method of cultivating sprout ginseng.
According to claim 1,
The culture step is carried out for 3 to 6 weeks, sprout ginseng cultivation method.
A sprout ginseng grown according to the method of claim 1.
The method of claim 11,
The cultivated sprout ginseng will contain a compound K of 1,000 ug / g or more, sprout ginseng.

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