KR20200048158A - Composition for antioxidant or improving skin wrinkle containing defatted Perilla frutescens extract - Google Patents

Abstract

The present invention relates to a composition for antioxidation or skin wrinkle amelioration containing a perilla seed dreg extract. More specifically, the present invention relates to a cosmetic and food composition for antioxidation or skin wrinkle amelioration containing the perilla seed dreg extract. According to the present invention, since the perilla seed dreg extract has an effect of scavenging DPPH radicals and inhibiting the activity of collagenase and elastase, the perilla seed dreg extract has excellent antioxidant and skin wrinkle amelioration effects, and thus can be used in various fields in cosmetics and foods for delaying skin aging.

Description

Composition for antioxidant or improving skin wrinkle containing defatted Perilla frutescens extract}

The present invention relates to a composition for improving antioxidant or skin wrinkles including perilla extract, and more particularly, to a cosmetic and food composition for improving antioxidant or skin wrinkles comprising perilla extract.

Skin is largely divided into epidermis, dermis and subcutaneous tissue. In the dermis, there are important appendages of the skin such as blood vessels, lymphatic system, pores, and fibroblasts, and the thick layer, which is 15-40 times the thickness of the epidermis, occupies most of the skin (Peyrefitte, 2011). The dermis consists of collagen, a crosslinked fiber, and elastin, an elastic fiber, which are synthesized in fibroblasts. Collagen is a long fiber that serves to support, bond, and interface the body. Collagen acts to resist pressure and external forces on the skin. Elastin, on the other hand, maintains the elasticity of the skin like a spring. Collagen and elastin form a mesh structure to maintain skin elasticity (Peyrefitte, 2011).

Wrinkles appear as the skin ages, and the number, depth, and range increases with age. Aging is classified into endogenous and exogenous aging. Endogenous aging is a phenomenon in which the structure and physiological functions of the skin continue to decline with age. Aging caused by external factors is caused by long-term exposure to ultraviolet rays (Sung et al., 2008).

It has been reported that elastase produced in fibroblasts plays an important role in the three-dimensional distortion of skin elastic fibers. Increased elastase activity contributes to skin wrinkle formation by reducing elastin and collagen in the skin. Since the activity of elastase increases after irradiation with ultraviolet rays, the change in the activity of elastase is known to be a major cause of skin elasticity reduction and wrinkle formation due to ultraviolet rays (Oh et al., 2009).

Perilla ( Perilla frutescens var. japonica Hara) is a perennial herbaceous plant belonging to the genus Lamiaceae, which is native to eastern Asia and has been cultivated in regions such as Korea, Japan, and northeastern China. It grows well on unfertile soils. The shape of perilla seeds is round and the size is around 2.5 mm × 2.3 mm, and there are many colors depending on the type, but there are many melanoma (Yeo et al., 1998). Perilla seeds have a very high content of fats and oils, so they have a tonic effect as well as fats and oils.

The important component of perilla fat is glyceride of oleic acid and linolenic acid, and the content of polyunsaturated fatty acid (PUFA) is high, making it unstable to rancidity (Mo, 1975). Since it can be stored without rancidity for a long period of time, it has been reported that special antioxidants exist along with the air-blocking effect of the seed itself (Lee et al., 1993; Kim et al., 1981; Yoon et al., 1993; Lee, 1993-7; Hong et al., 1997).

Accordingly, the present inventors completed the present invention by confirming the remarkable antioxidant activity and skin wrinkle improvement effect of the perilla extract as a result of research to discover materials having antioxidant and anti-wrinkle activity.

Therefore, an object of the present invention is to provide a cosmetic and food composition for improving antioxidant or skin wrinkles containing perilla extract as an active ingredient.

In order to achieve the above object, the present invention provides a cosmetic composition for antioxidant comprising perilla extract as an active ingredient.

In addition, the present invention provides a food composition for antioxidant containing perilla extract as an active ingredient.

In addition, the present invention provides a cosmetic composition for preventing or improving skin wrinkles comprising perilla extract as an active ingredient.

In addition, the present invention provides a food composition for preventing or improving skin wrinkles containing perilla extract as an active ingredient.

The perilla extract according to the present invention has the effect of scavenging DPPH radicals and inhibiting the activity of collagenase and elastase, and thus has excellent antioxidant and skin wrinkle improvement effects. It can be used in various ways.

1 is a view showing the results of measuring the total polyphenol and total flavonoid content of the perilla extract ethanol extract according to the present invention.
2 is a view showing the results of evaluating the cytotoxicity of perilla extract ethanol extract according to the present invention.
3 is a view showing the results of analyzing the antioxidant activity of perilla extract ethanol extract according to the present invention.
4 is a view showing the results of analyzing the collagenase inhibitory activity of perilla extract ethanol extract according to the present invention.
5 is a view showing the results of analyzing the elastase inhibitory activity of perilla extract ethanol extract according to the present invention.

Hereinafter, the present invention will be described in detail.

According to an aspect of the present invention, antioxidant for containing perilla extract as an active ingredient; Or for preventing or improving skin wrinkles; Provided is a composition. The composition includes cosmetic and food compositions.

In the present invention, the "extract" is extracted from perilla extract using a suitable solvent, and includes, for example, a crude extract of perilla extract, a polar solvent soluble extract, or a non-polar solvent soluble extract. In addition, the extract is a concept including all of the extracts, fractions, their diluents, concentrates or dried products obtained at each stage of extraction, fractionation or purification.

The perilla extract, which is an active ingredient of the composition of the present invention, can be obtained by a conventional extraction method or can be used by purchasing a commercially available one.

The perilla extract can be obtained by extraction, separation and fractionation from nature using extraction, separation and fractionation methods known in the art.

As a suitable solvent for obtaining the perilla extract, water or an organic solvent may be used, and any organic solvent acceptable in cosmetics and food may be used. For example, as the solvent, alcohols having 1 to 4 carbon atoms, such as water, methanol, ethanol, propanol, isopropanol, butanol, alone or two or more, It can be used by mixing. Preferably, ethanol may be used as the solvent.

The extraction temperature is preferably room temperature. In addition, various methods such as hot water extraction, immersion, cold immersion extraction, reflux cooling extraction, solvent extraction, water vapor distillation, ultrasonic extraction, elution, and compression may be used, but are not limited thereto, preferably ethanol. It is to use the immersion extraction method using.

The perilla extract can be obtained by immersing, heating and filtering at room temperature by a conventional method known in the art to obtain a liquid, or further evaporating, spray drying or freeze-drying the solvent. In addition, the perilla extract can be prepared in powder form by additional processes such as distillation under reduced pressure and freeze drying or spray drying. In addition, the extract or fraction is further purified by using various chromatography such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography, etc. You can also get

Therefore, the perilla extract used in the present invention is a concept that includes all of the extracts, fractions and refinements, their dilutions, concentrates or dried products obtained at each stage of extraction, fractionation or purification.

In one embodiment of the present invention, the perilla extract was prepared by milking perilla and collecting and drying by-products, and ethanol was extracted from the dried perilla extract.

In another embodiment of the present invention, the composition preferably contains perilla extract at a concentration of 0.1 to 1000 mg / ml, more preferably 10 to 1000 mg / ml, and most preferably 200 mg / ml.

The composition of the present invention may further contain one or more known active ingredients having an antioxidant or skin wrinkle improvement effect together with perilla extract.

In the present invention, "antioxidation" is not limited as meaning the efficacy to slow down, block or prevent the oxidation process known in the art.

In the present invention, “skin wrinkles” refers to a phenomenon in which skin elasticity is reduced and skin folds due to skin aging, and the main cause of skin wrinkles is due to increased activity of collagenase and elastase. It is known for the degradation of collagen and elastin.

In the present invention, "prevention" refers to all actions to prevent the phenomenon by eliminating or prematurely detecting the cause of a phenomenon by the composition of the present invention.

In the present invention, "improvement" means any action in which the function of a biological metabolism, cell or organ is advantageously changed by the composition of the present invention.

The perilla extract according to the present invention has excellent DPPH radical scavenging ability and inhibits the activity of collagenase and elastase. This means that not only the antioxidant effect, but also excellent in preventing or improving skin wrinkles, perilla extract of the present invention is for antioxidant; Or for preventing or improving skin wrinkles; It can be usefully used as a composition.

The cosmetic composition of the present invention may further include conventional adjuvants and carriers, such as antioxidants, stabilizers, solubilizers, vitamins, pigments, fragrances, etc., which are commonly used in cosmetic compositions in addition to the active ingredients. For example, the cosmetic composition may further include auxiliary components such as glycerin, butylene glycol, polyoxyethylene cured castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate, allantoin, and the like. .

Since the cosmetic composition of the present invention is basically applied to the skin, it can be prepared in any formulation that is conventionally prepared with reference to cosmetic compositions in the art. For example, it can be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray, etc. It is not limited to this. More specifically, it can be prepared in the form of a flexible lotion, nutrient lotion, nutrient cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, mask pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. may be included as carrier components. have.

When the formulation of the present invention is a powder or a spray, as a carrier component, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be included. In particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane / Propellants such as butane and dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a carrier component may include a solvent, a solubilizing agent, an emulsifying agent, etc., specifically water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene Glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid ester of sorbitan, and the like.

When the formulation of the present invention is a suspension, liquid diluents such as water, ethanol, and propylene glycol as carrier components; Suspension agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, trakant, and the like.

When the formulation of the present invention is a surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivatives, methyltaurate, sarcosinate, fatty acid amide as a carrier component Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, ethoxylated glycerol fatty acid esters, and the like.

The food composition of the present invention may be a health functional food, food additive or dietary supplement. When the composition of the present invention is a food additive, perilla extract may be added as it is, or may be suitably used according to a conventional method such as being used in combination with other food or food ingredients.

The type of food is not particularly limited, but examples of foods to which the perilla extract of the present invention can be added are meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice creams Dairy products, including various soups, beverages, teas, drinks, alcoholic beverages, vitamin complexes, etc., and include all healthy foods in the ordinary sense.

When the food composition of the present invention is prepared as a beverage, it may include various components such as various flavoring agents or natural carbohydrates, such as a conventional beverage. The natural carbohydrates include monosaccharides such as glucose and fructose; Disaccharides such as maltose and sucrose; Natural sweeteners such as dextrin and cyclodextrin; Synthetic sweeteners such as saccharin and aspartame can be used. The natural carbohydrate is contained in 0.01 to 10% by weight, preferably 0.01 to 0.1% by weight relative to the total weight of the food composition of the present invention.

The food composition of the present invention is a variety of nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonic acid Carbonation agents used in beverages, and the like, and may include, but are not limited to, natural fruit juice, fruit juice beverage, and flesh for preparing vegetable beverages. These ingredients may be used independently or in combination. The additive ratio is not particularly limited, but is preferably included in the range of 0.01 to 0.1% by weight relative to the total weight of the food composition of the present invention.

In the case of long-term intake for health and hygiene purposes or for health control purposes, the food composition of the present invention can be taken for a long time because there is no problem in terms of safety.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as limited by these examples.

Comparative example  One. Perilla Hydrothermal  Extract preparation

The perilla extract used in the production of perilla extract was used by drying and collecting the remaining by-products after milking raw perilla from Eumseong Comega, Chungbuk. The dried perilla gourd was pulverized to 20 mesh or less to prepare perilla gourd powder. 10 times distilled water was added to the weight of the perilla powder, followed by hot water extraction at room temperature for 24 hours. Then, the sample was filtered (Whatman No2, Maidstone, England) and concentrated under reduced pressure (N-1000S-WD, Eyela Co., Tokyo, Japan), followed by freeze-drying (FDU-1100, Eyela Co., Tokyo, Japan) and perilla. Completed the hot water extract (hereinafter referred to as WE).

Example  One. Perilla  Ethanol extract preparation

The perilla extract ethanol extract was prepared using the perilla extract powder prepared in Comparative Example 1. The dried perilla gourd was pulverized to 20 mesh or less to prepare perilla gourd powder. Perilla powder and solvent were mixed at a ratio of 1:10 using 70% ethanol, and extracted at room temperature for 72 hours. Then, the sample was filtered (Whatman No2, Maidstone, England) and concentrated under reduced pressure (N-1000S-WD, Eyela Co., Tokyo, Japan), followed by freeze-drying (FDU-1100, Eyela Co., Tokyo, Japan) and perilla. A complete ethanol extract (hereinafter referred to as EE) was completed.

Example  2. Perilla  Determination of total polyphenol and total flavonoid content of ethanol extract

The total polyphenol and total flavonoid content of the perilla extract ethanol extract prepared in Example 1 was measured.

To compare the total polyphenol content, the Folin and Denis method (Folin and Denis, 1912) was applied. Perilla ethanol extract was mixed by adding 2 mL of a 2-fold diluted folin reagent (Sigma Co., St. Louis, MO, USA) to 2 mL of the solution diluted by concentration. The mixture was left for 3 minutes, and then 2 mL of 10% Na ₂ CO ₃ (Sigma Co.) was added and reacted for 1 hour, followed by UV / Visible spectrophotometer (UVIKON 922, Kontran Co., Milan, Italy) 700 The content was calculated from the standard curve prepared by measuring the absorbance at nm. In this case, the standard curve using gallic acid (Sigma Co.) was obtained by measuring the absorbance at 700 nm in the same way as the final concentration of gallic acid was 0, 5, 25, 50, 75 μg / mL. Did.

The total flavonoid content was measured by applying the method of Nieva Moreno et al. (Nieva et al., 2000). To a mixture of 0.1 mL of each sample and 0.9 mL of 80% ethanol, add 0.5 mL of 10% (Sigma Co.), 1 M aluminum nitride (Sassima Co.) 0.1 mL, and 4.3 mL of 80% ethanol to 4.3 mL. After reacting for minutes, absorbance was measured at 415 nm. At this time, the total flavonoid content was calculated from a standard curve prepared using quercetin (Sigma Co.).

The results of measuring the total polyphenol and total flavonoid content are shown in FIG. 1.

1, it was confirmed that the total polyphenol content of the perilla extract ethanol extract was 48.32 mg / g, and the total flavonoid content was 17.32 mg / g.

Example  3. Perilla  Toxicity evaluation of ethanol extract

The microglial cell line BV-2 cells used for toxicity evaluation were purchased from Harvard University. Cultures of BV-2 cells were cultured in 10% fetal bovine serum (FBS; Gibco, BRL, USA), 100 μg / mL penicillin (Gibco, BRL, USA) and 100 μg / mL streptomycin (Gibco, BRL, USA) containing RPMI1640 medium (Gibco, BRL, USA) was used, and cultured once every 2-3 days in a 37 ° C incubator with 5% CO ₂ .

To confirm the effect of perilla ethanol extract on cell survival in BV-2 cells stimulated with LPS (Lipopolysaccharide), the cell viability was determined by MTT (3- [4,5-Dimethylthiazol-2-yl] -2,5-diphenyl -tetrazolium bromide). Cells (1X10 ⁵ cell / mL) were dispensed into a 96-well plate in 180 μL increments and cultured in a 5% CO ₂ incubator for at least 12 hours. The cultured cells were treated with perilla ethanol extract at various concentrations (10, 25, 50, 75, 100 μg / ml) and cultured for 24 hours. The culture solution was removed, and 200 μL of a medium containing 0.5 mg / mL MTT was added, and further cultured for 4 hours to reduce MTT. Thereafter, the culture solution was removed, and 100 μL of DMSO (dimethylsulfoxide) was added to dissolve the generated formazone crystals, and absorbance was measured at 540 nm using an ELISA reader. The control group did not process the LPS and the sample, and the comparison group treated the perilla extract hot water extract prepared in Comparative Example 1. The cell viability of the experimental group was expressed as a percentage (%) of the measured absorbance value based on the control group. The cytotoxicity evaluation results are shown in FIG. 2.

As shown in Figure 2, the perilla ethanol extract treatment group confirmed that the cell viability was 95% or more. The above results indicate that the perilla ethanol extract has little cytotoxicity.

Example  4. Perilla  Analysis of antioxidant activity of ethanol extract

DPPH (α-α-Diphenyl-β-picrylhydrazyl) has the property of easily accepting hydrogen atoms in an unstable nitrogen atom of hydrazyl, so it reacts with antioxidants and loses its own color by accepting hydrogen atoms. Can be used to measure the degree of antioxidant capacity.

To measure DPPH radical scavenging activity, 30 μl of various concentrations of perilla ethanol extract (10, 20, 40, 80, 100, 200 μg / ml) and DPPH solution with a concentration of 60 μM (DPPH dissolved in methanol) 30 μl was mixed and reacted at room temperature for 2 minutes. After the mixed solution was transferred to a tube, electron spin resonance (ESR) was measured with a spectrophotometer (JES-PX 2300, Jeol Co., Japan). At this time, the measurement conditions of the ESR spectrophotometer were 336.5 ± 5 mT in the case of a magnetic field, 5 mW in the microwave power and 9.41 GHz in the modulation frequency. DPPH radical inhibitory activity on the modulated sample was calculated by the following calculation formula. The control group was treated with methanol, and the comparative group was treated with perilla extract hot water extract prepared in Comparative Example 1. The results of the DPPH radical scavenging activity analysis of the experimental group are shown in FIG. 3.

As shown in Figure 3, the concentration of 10 μg / ml perilla extract ethanol extract was confirmed that the DPPH radical scavenging ability is about 68%. In addition, it was confirmed that the perilla ethanol extract having a concentration of 20 μg / ml or more had a DPPH radical scavenging ability of 80% or more. On the other hand, it was confirmed that the perilla perilla hot water extract had a low DPPH radical scavenging ability.

Example  5. Perilla  Of ethanol extract Collagenase ( collagenase ) Inhibition activity assay

The biosynthesis of collagen is reduced by various factors, and decomposition is accelerated by external factors such as increased stress caused by various harmful environments and increased free radicals caused by UV rays, and wrinkles are generated as the skin matrix is destroyed. Collagenase is an enzyme that breaks down collagen in the skin matrix, and can inhibit the formation of wrinkles by inhibiting its activity.

Measurement of the inhibitory activity of collagenase in perilla extract ethanol extract was measured by the method of Kim et al. (2004). The reaction solution for the analysis of collagenase inhibitory activity was prepared by adding 4 mM CaCl ₂ to 0.1 M Tris-HCl buffer (pH 7.5). 0.25 ml of the reaction solution, 0.1 ml of 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg solution having a concentration of 0.3 mg / ml and ethanol extract of perilla extract (20, 40, 80, 100 mg / ml) of various concentrations Mixed. 0.15 ml of collagenase (0.2 mg / ml) was added to the mixture and left at room temperature for 20 minutes. Thereafter, 0.5 ml of 6% citric acid was added to stop the reaction, and 1.5 ml of ethyl acetate was added, and the absorbance of the supernatant was measured at 320 nm. The control group did not process the extract, and the comparison group treated the perilla extract hot water extract prepared in Comparative Example 1. The collagenase inhibitory activity was expressed as a decrease in absorbance between the experimental group and the control group. The results of the analysis of collagenase inhibitory activity are shown in FIG. 4.

As shown in Figure 4, the concentration of 20 mg / ml perilla extract ethanol extract had a collagenase inhibitory activity was 4.1%, it was confirmed that the collagenase inhibitory activity increases as the concentration of the extract increases. In particular, it was confirmed that the collagenase inhibitory activity of the experimental group having the concentration of perilla extract ethanol extract of 80 and 100 mg / ml was 26.7 and 31.2%, respectively. On the other hand, it can be seen that the comparative group perilla extract hot water extract has very low collagenase inhibitory activity. From the above results, the perilla ethanol extract has excellent collagenase inhibitory activity, and means that it is possible to suppress wrinkle formation through collagenase inhibition.

Example  6. Perilla  Of ethanol extract Elastase ( elastase ) Inhibition activity assay

Elastase is an enzyme that breaks down elastin, a protein, and is a non-specific hydrolase that can break down another substrate, collagen. In the dermal tissue of the skin, collagen and elastin related to the elasticity of the skin form a mesh structure. Elastin is decomposed through the action of elastase, which is the main cause of wrinkle formation, and thereby, wrinkles are generated by breaking the network structure in the dermal tissue. Inhibiting elastase has the effect of improving skin wrinkles, and ursolic acid has been reported as an elastase inhibitor (Tsuji et al ., 2001).

Elastase inhibitory activity of perilla extract ethanol extract was analyzed by measuring the production of P-nitroanilide for 30 minutes at 37 ° C using N-succinyl- (L-Ala) 3-p-nitroanilide as a substrate (Cannell et al. ., (1998)). Specifically, 0.1% of perilla ethanol extracts of various concentrations (20, 40, 80, 100 mg / ml) were taken into test tubes, and the elastase solution dissolved in Tris-HCl buffer (pH 8.6) having a concentration of 50 mM. (Type I: derived from porcine pancreas, 0.6 units / ml) 0.05 ml was added to prepare a reaction solution. 0.1 ml of N-succinyl- (L-Ala) 3-p-nitroanilide (1 mg / ml) dissolved in 50 mM Tris-HCl buffer (pH 8.6) was added to the reaction solution, followed by reaction for 30 minutes. After the reaction was completed, the absorbance of the reaction solution was measured at 410 nm using a microplate reader. The control group did not process the extract, and the comparison group treated the perilla extract hot water extract prepared in Comparative Example 1. The inhibitory activity of elastase was expressed as the decrease in absorbance between the experimental group and the control group. The results of the analysis of the elastase inhibitory activity are shown in FIG. 5.

As shown in FIG. 5, the concentration of 20 mg / ml perilla extract ethanol extract had an elastase inhibitory activity of 3.8%, and it was confirmed that the elastase inhibitory activity increased as the concentration of the extract increased. In particular, it was confirmed that the elastase inhibitory activity of the experimental group in which the concentration of perilla extract ethanol was 100 mg / ml was 35.7%. On the other hand, it can be seen that the perilla perilla hot water extract has a very low elastase inhibitory activity. The above results indicate that the perilla ethanol extract has excellent elastase inhibitory activity and can suppress wrinkle formation through elastase inhibition.

Overall, the present inventors confirmed that the perilla ethanol extract has excellent DHHP radical scavenging activity, inhibitory activity of collagenase and elastase. This means that the perilla extract ethanol extract is not only toxic to cells, but also has significant antioxidant and anti-wrinkle activity. The perilla extract ethanol extract of the present invention can be used in various fields in skin improvement, antioxidant and wrinkle improvement. .

Hereinafter, the present invention will be described in more detail through formulation examples. The formulation examples are only for illustrating the present invention, and the scope of the present invention is not to be construed as being limited by the formulation examples.

Formulation example  1. Manufacture of flexible cosmetic (skin lotion)

Perilla ethanol extract 0.5%

Beta-1,3-glucan 1.0%

Butylene glycol 2.0%

Propylene glycol 2.0%

Carboxyvinyl polymer 0.1%

PG-12 nonylphenyl ether 0.2%

Polysorbate 80 0.4%

Ethanol 10.0%

Triethanolamine 0.1%

Preservatives, pigments, flavors

Purified water to 100%

Formulation example  2. Nutritional makeup ( Milk lotion )

Perilla ethanol extract 0.5%

Beta-1,3-glucan 1.0%

Wax 4.0%

Polysorbate 60 1.5%

Sorbitan sesquioleate 1.5%

Liquid paraffin 0.5%

Caprylic / Capric Triglyceride 5.0%

Glycerin 3.0%

Butylene glycol 3.0%

Propylene glycol 3.0%

Carboxyvinyl polymer 0.1%

Triethanolamine 0.2%

Preservatives, pigments, flavors

Purified water to 100%

Example  3. Preparation of nutrition cream

Perilla ethanol extract 1.0%

Beta-1,3-glucan 5.0%

Mill blade 10.0%

Polysorbate 60 1.5%

Easy Easy 60 Castor Oil 2.0%

Solbitan sesquioleate 0.5%

Liquid paraffin 10.0%

Squalane 5.0%

Caprylic / Capric Triglyceride 5.0%

Glycerin 5.0%

Butylene glycol 3.0%

Propylene glycol 3.0%

Triethanolamine 0.2%

Preservatives, pigments, flavors

Purified water to 100%

As described above, since a specific part of the present invention has been described in detail, it is obvious to those skilled in the art that this specific technique is only a preferred embodiment, and the scope of the present invention is not limited thereby. something to do. Therefore, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (7)

A cosmetic composition for antioxidant, comprising perilla extract as an active ingredient.
According to claim 1,
The perilla extract is extracted with one or more solvents selected from the group consisting of water, alcohols having 1 to 4 carbons and mixed solvents thereof, and antioxidant cosmetic composition.
According to claim 2,
The perilla extract was extracted with ethanol as a solvent, antioxidant cosmetic composition.
According to claim 1,
The perilla extract has a concentration of 0.1 to 1000 mg / ml, cosmetic composition for antioxidant.
A food composition for antioxidant, comprising perilla extract as an active ingredient.
Cosmetic composition for preventing or improving skin wrinkles, including perilla extract as an active ingredient.
Food composition for preventing or improving skin wrinkles, including perilla extract as an active ingredient.

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