KR20190119312A - Insecticidal and Miticidal Composition of Red Poultry Mite Using an Extract of Gleditsiae Fructus - Google Patents

Abstract

The present invention discloses a pesticidal and acaricide composition for Dermanyssus gallinae using a fruit extract of Gleditsia sinensis Lamark or Gleditsia japonica Miquel. The fruit extract of Gleditsia sinensis Lamark or Gleditsia japonica Miquel shows an average mortality effect of 89.6% in a killing effect test for Dermanyssus gallinae.

Description

Insecticidal and Miticidal Composition of Red Poultry Mite Using an Extract of Gleditsiae Fructus}

The present invention relates to an insecticidal and acaricide composition for chicken ticks using the cooperative extract.

Chemical pesticides, which have been in use for decades, are resistant to pests and can only be controlled by using more pesticides or more lethal pesticides over time. This not only makes the environmental problem more serious, but also causes serious social problems that act as fatal hazards to the human body that consumes crops directly.

Recently, pesticides harmful to humans have been found in eggs, which has become a major social problem. This appears to be due to insecticides sprayed to control chicken ticks, and eventually, the consumption of eggs is reduced, and even the laying hen industry, which accounts for a large portion of agri-food production.

Chicken ticks ( Dermanyssus gallinae ) are a globally distributed external parasite that cause high economic losses in poultry farms. This species is a parasitic and vampire pest that lives on chickens, wild birds, and other rodents, and is the most important blood-sucking parasite because it can thrive in broilers and lead to anemia, reduced productivity and even death in laying hens. It is also a mediator of avian spirochetes, chicken pox virus, Newcastle virus, Chubaekri, poultice and cholera.

As such, it has relied primarily on chemical control to control the most threatening people in poultry farms. The most frequently used pesticides include carbaryl, diazinon, dichlorvos, permethrin, and anti-dioxide.

At present, the drugs used for controlling chicken ticks include pyrethroid, carbamate, and organophosphorus. In recent years, phenylpyrazoe-based pypronyl (phenylpyrazoe) fipronil) is also used. A domestic animal drug company has launched a product called "Wagupuri" containing formic acid and 2-tert-butyl-5- (4-tert-butylbenzylthio) -4-chloropyridazin-3 (2H) -one. Resistant "wagoupriblue" containing Bifenthrin (2-methyl [1,1'-biphenyl] -3-yl-methyl3- (2-chloro-3,3,3-trifluoro-1-propenyl2 and 2-dimethylcyclopropanecarboxylate "We launched a product called.

On the other hand, some laying hen farms use pesticides, which are negative organic chemical pesticides, indiscriminately. Overseas, plant oil is used to control the fumigation method, and chemical acaricide is used as in Korea. In other words, there are many cases of using chicken chemical mites or replacing organic synthetic pesticide insecticides that negatively suppress pests of crops, so harmful chemicals or pesticides may remain in livestock produced on farms. It can adversely affect the health of the people you eat.

Accordingly, there is an urgent need for low-toxic, non-toxic or pollution-free natural pesticides or biological pesticides to realize eco-friendly agriculture. Recently, despite the active research of biopesticides such as natural pesticides and natural microorganisms to replace chemical pesticides, most natural pesticides and natural microorganisms have a weak control effect or extremely limited effects. In addition, due to the high price, it is not effective and economically unfavorable from farmers, so it has not succeeded as a substitute for chemical pesticides to realize eco-friendly agriculture. Therefore, there is a demand for the development of pesticides that are harmless to humans and animals, and are environmentally friendly, easy to handle, low in production price, and high in control value.

Korean Patent No. 20-2014-0036864 discloses a natural insecticide composition comprising a plant extract as an active ingredient. This prior art discloses a natural insecticide composition comprising a mixture of filamentous extract, rhododendron extract, oak extract, yellowwood extract and green tea extract, and mosquitoes, flies and ticks as an environmentally friendly agent that does not affect the human body and the environment. Increasing the insecticidal insects such as and reveals that it can be used in a variety of formulations.

Korean Patent No. 10-2016-0074410 discloses acaricide composition using a natural plant extract. This composition describes a chicken mite acaricide composition comprising a mixture of nim oil, ginseng extract, cinnamon extract and ginkgo biloba extract as an active ingredient. It also discloses the optimum conditions of surfactants, vegetable oils and antioxidants that can increase the effectiveness of using such compositions as acaricide compositions.

On the other hand, Korean Patent Application No. 10-2014-0190372 discloses a pest control composition comprising a natural vegetable extract as an active ingredient. Here, Chrysanthemum cinerariaefolium, red ginseng (Sophoraflavescens), tobacco (Nicotiana tabacum L.), wild tobacco (Nicotiana Glauca), mulberry (Melia azedarah var. Japonica), garlic (Allium sativum L.), irises (Acorus) Disclosed is a pest control composition comprising an extract of calamus L.), Marigold (Tagetes erecta) and Ginkgo (Ginkgo biloba L.) as an active ingredient and a method for controlling pests using the same.

Insecticides with these characteristics have the advantage of being able to evade pests and additionally control pests hiding in hideouts where general insecticides are difficult to penetrate directly. It is known that more than 35 kinds of substances are used to control chicken ticks, and some of them have excellent effects but are inappropriate for food safety and environmental aspects. In addition, repetitive and long-term use of drugs has led to the development of resistant ticks, and there are no clear alternatives to replace them.

Consumers' dissatisfaction with the use of indiscriminate antibiotics and pesticides has increased their awareness of safe foods, raising concerns about the possibility of antibiotics or pesticides remaining in poultry or poultry eggs. At the same time, there is an increasing demand for control drugs for efficient and healthy poultry farming.

Thus, the present inventors have tried to develop a pesticide composition for fertilizing chicken ticks in a more environmentally and economically more efficient way in livestock farms, it was confirmed that the extract of Gleditsiae Fructus shows insecticidal activity against chicken ticks.

It is an object of the present invention to provide a pesticidal and acaricide composition for chicken ticks using the cooperative extract.

Other and specific objects of the present invention will be presented below.

The present invention was completed by confirming that the coarse water extract shows a lethal effect of 89.6% for chicken ticks as in the following examples.

The insecticidal and acaricide composition for chicken mite of the present invention is characterized in that it comprises crude extract as an active ingredient.

Johyup is an herb listed in the Korean Pharmacopoeia Herbal Medicines Standard and refers to the fruit of Gleditsia sinensis Lamark or Gleditsia japonica Miquel belonging to the legume (Leguminosae) according to the standard.

In the present specification, "coarse extract" refers to the coarse water to be extracted water, lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.), methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate , Supercritical extraction solvents such as extracts obtained by leaching with N, N-dimethylformamide (DMF), dimethylsulfoxide (DMSO), 1,3-butylene glycol, propylene glycol or mixed solvents thereof, carbon dioxide, pentane Means an extract obtained by using or fractions obtained by fractionating the extract, and the extraction method may be any method such as cooling, refluxing, heating, ultrasonic radiation, supercritical extraction in consideration of the polarity, degree of extraction, and degree of preservation of the active substance. Applicable In the case of the fractionated extract, the fraction obtained by suspending the extract in a specific solvent and mixing and standing with a solvent having a different polarity, the crude extract is adsorbed on a column filled with silica gel and the like, and then a hydrophobic solvent, a hydrophilic solvent, or a mixed solvent thereof is added. It is meant to include fractions obtained by mobile phase. In addition, the meaning of the extract includes a concentrated liquid extract or solid extract in which the extraction solvent is removed in a manner such as freeze drying, vacuum drying, hot air drying, spray drying, and the like. Preferably it means an extract obtained using water, ethanol or a mixed solvent thereof as the extraction solvent.

In addition, the term "active ingredient" as used herein means a component that can exhibit the desired activity alone or in combination with a carrier that is not active itself.

The insecticidal and acaricide composition of the present invention can be formulated into various formulations known in the art, including the above-mentioned effective ingredient. Specifically, they may be formulated into solutions, emulsions, hydrating agents, aqueous suspensions, oily suspensions, powders, powders, pastes, soluble powders, soluble granules, spray granules, aerosols, sprays and the like.

The coarse extract as an active ingredient may be included in these formulations in the range of 0.01 to 20% by weight, preferably 0.1 to 10% by weight, depending on the type of preparation and the preparation method thereof.

When formulated, it may be formulated to include carriers, emulsifiers, and other formulation auxiliaries, depending on the formulation type and the formulation method thereof.

As the carrier, any carrier known in the art may be used. Suitable solid carriers include in particular ammonium salts and ground natural minerals such as kaolin, clay, talc, chalk, quartz, attapulgite, montmorillonite or diatomaceous earth, finely divided silica, alumina, silicates and the like. Solid granules suitable for granulation are, for example, granulated and classified natural rocks such as calcite, marble, pumice, calcite and dolomite, granules of organic substances such as paper, sawdust, coconut husks, corncobs and tobacco. Suitable liquid carriers include, for example, water, alcohols such as methanol and ethanol, ketones such as acetone and methyl ethyl ketone, aromatic hydrocarbons such as benzene and toluene, xylene and ethylbenzene, hexane and cyclohexane Aliphatic hydrocarbons such as kerosene and diesel, esters such as ethyl acetate and butyl acetate, nitriles such as acetonitrile and isobutyronitrile, ethers such as diisopropyl ether and dioxane, N, N Acid amides such as -dimethylformamide and N, N-dimethylacetoamide, halogenated hydrocarbons such as dichloromethane and trichloroethane, dimethyl sulfoxide, vegetable oils such as soybean oil and cottonseed oil. Examples of suitable gas carriers include butane gas, LPG (liquefied petroleum gas), dimethyl ether, carbon dioxide gas and the like.

Emulsifiers can be used for both ionic and nonionic emulsifiers. Suitable emulsifiers include sulfuric acid alkyl esters, alkylsulfonic acid salts, alkylarylsulfonic acid salts, alkylaryl ethers and polyoxyethylenes thereof adducts), polyethylene glycol ethers, polyhydric alcohol esters, sugar alcohol derivatives, and the like.

Examples of the adjuvant for the preparation include fixing agents and stabilizers.

As the fixing agent, for example, polysaccharides such as casein, gelatin and starch, cellulose derivatives such as starch, gum arabic and carboxymethylcellulose, alginic acid and the like), lignin derivatives and bentonite ), Synthetic water-soluble polymers (polyvinyl alcohol, polyvinylpyrrolidone, polyacrylic acid, etc.), and the like, and as stabilizers, for example, acidic isopropyl phosphate (PAP), 2,6-di-tert -Butyl-4-methylphenol (BHT), a mixture of 2-tert-butyl-4-methoxyphenol and 3-tert-butyl-4-methoxyphenol (BHA), vegetable oils, mineral oils, fatty acids and esters thereof Can be mentioned.

Formulation types and formulation methods are well known in the art, and more specifically, Crop Life International and in Pesticide Specifications, Manual on development and use of FAO and WHO specifications for pesticides, FAO Plant Production and Protection Papers-173, prepared by FAO / WHO Joint Meeting on Pesticide Specifications, 2004, ISBN: 9251048576].

In another aspect, the present invention relates to a method for killing and killing chicken ticks.

The method of the present invention comprises the step of contacting the insecticidal and acaricide composition of the present invention as described above with a chicken tick.

In the method of the present invention, the contact can be made by spraying, spraying and applying the composition of the present invention directly to chickens infected with chicken ticks. In this case, only the insecticide composition itself of the present invention can be used, but may be used in combination with other insecticides, nematicides, acaricides, animal feeds and the like.

As described above, according to the present invention can provide a pesticidal and acaricide composition for chicken ticks using the cooperative extract.

The composition of the present invention is more environmentally friendly in that it uses a coarse extract which is a natural product having an insecticidal and acaricidal effect, and has less safety problems due to pesticide residues.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

Example 1 Preparation of Crude Extract

10-fold weight of 70% ethanol was added to the dry powder of crude cotyledon and leached at room temperature for 24 hours, followed by filtration, concentration under reduced pressure, and lyophilization to obtain a powdery crude coarse extract.

Example 2 Insecticidal and acaricide activity against chicken ticks

20 chicken tick specimens were inoculated with a brush in a breeding dish (50 × 15 mm), and immediately inoculated with the sample (the powdery coarse extract was diluted in water at a 1/100 (w / v) dilution ratio. Sample) was directly sprayed three times with a spray. The control was sprayed with water. The lid was then covered and sealed with Parafilm to prevent ticks from crawling out. The death rate was determined by counting dead individuals after 24 hours. However, when the mortality rate of the control group was 5% or more, Abbott's formula was used.

Corrected insecticidal rate = (mortality in treated group-mortality in control) / (100-mortality in control) × 100

The experiments were carried out on three insect culture dishes and the results were calculated using the mean and standard deviation. The percent mortality of the leaf extracts was 89.6 ± 3.7.

Claims (5)

Insecticide and acaricide composition for chicken mite containing the coarse water extract as an active ingredient.
The method of claim 1,
The extract is an insecticide / caricide composition, characterized in that the extraction using water, a lower alcohol of C1 to C4 or a mixture thereof as a solvent.
The method of claim 1,
The extract is a pesticide / caricide composition, characterized in that the extraction using water, methanol, ethanol or a mixture thereof as a solvent.
The method of claim 1,
The extract is an insecticide and acaricide composition, characterized in that obtained by extracting the bark fruit.
The insecticide and acaricide method of the chicken mite which comprises contacting the insecticide composition of any one of Claims 1-4 with a chicken tick.