KR102662090B1 - Composition and Method for Controling Mosquito Using an Extract of Lindera strychnifolia - Google Patents

Abstract

The present invention discloses a composition for controlling mosquitoes and a method for controlling mosquitoes using an extract of the Chinese herbal medicine, which has an insecticidal effect against mosquitoes.

Description

Composition and Method for Controlling Mosquito Using an Extract of Lindera strychnifolia}

The present invention relates to a composition for controlling mosquitoes using Lindera strychnifolia extract and a method for controlling the same.

Mosquitoes, which disrupt sleep in the summer and greatly limit outdoor activities, are representative pests that transmit diseases to humans. About 3,500 species are known on Earth, and 55 species in 9 genera are known in Korea. After mating, mosquitoes generally engage in blood-sucking activities to obtain animal proteins to mature the eggs of adult female insects, and secrete saliva containing pathogens or viruses into the blood-sucking area. Through this saliva, mosquitoes transmit diseases such as malaria, encephalitis, yellow fever, dengue fever, and filariasis (Science, 257:37-38, 1992) ), especially Japanese encephalitis, which occurs frequently in Korea, is known to have a high mortality rate and to leave severe aftereffects such as mental retardation even if treated.

Organophosphorus pesticides and pyrethroid pesticides have been used to control mosquitoes, but the most widely used substance currently is diethyltoluamide (DEET). DEET was first developed by the U.S. military in 1946 and has been widely used to control various pests such as mosquitoes, flies, fleas, and ticks (Int. J. Pharm. 163:167-176, 1998). However, due to its high skin penetration, it can cause transdermal toxicity (Drug Metab. Dispos. 22:106-112, 1994) and cause diseases such as encephalopathy, acute manic psychosis, and cardiovascular disease. It can be done (Am. Mosq. Control Assoc. 11: 274-278, 1995). Therefore, its use is restricted for children, pregnant women, patients with low blood pressure, people with sensitive skin, etc. In particular, in the United States, the manufacture of mosquito repellent containing DEET at a concentration of 20% or more is prohibited.

For these reasons, recent efforts have been made to develop mosquito control agents using natural products with relatively few side effects, such as Achyranthes bidentata extract, Cyanotis arachnoidea extract, etc. (Korean Patent No. 10-1778051), Neem oil, etc. (Korean Patent Publication No. 10-2017-0049822), bay ( Pimenta racemosa ) extract, etc. (Korean Patent Publication No. 10-2004-0070903), clove oil, etc. (Korean Publication Patent) No. 10-2012-0014536), Hwangbyeok tree extract, etc. (Korean Patent No. 10-1470477), citronella essential oil, etc. (Korean Published Patent No. 10-2014-0042008) are mosquito control agents derived from natural products. It is proposed.

The present invention also discloses a cucumber extract that has insecticidal activity against mosquitoes.

The purpose of the present invention is to provide a composition for controlling mosquitoes using a cucumber extract.

Another object of the present invention is to provide a method for controlling mosquitoes using cucumber extract.

Other or specific purposes of the present invention will be presented below.

As confirmed in the examples and experimental examples below, the present invention was completed by confirming the insecticidal effect of the cucumber extract on Aedes tiger mosquitoes and red house mosquitoes.

Therefore, the composition for controlling mosquitoes of the present invention is characterized by containing cucumber extract as an active ingredient.

In the present specification, the term "Five extract" refers to the leaves, stems, aerial parts, rhizomes, roots, underground parts, or mixtures thereof of the extract object, the leaves, stems, aerial parts, or mixtures thereof, with water and a lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.). , methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butylacetate, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol or these. refers to an extract obtained by leaching using a mixed solvent, an extract obtained by using a supercritical extraction solvent such as carbon dioxide, pentane, or a fraction obtained by fractionating the extract. The extraction method is based on the polarity, degree of extraction, and degree of preservation of the active substance. Considering this, any method such as cold immersion, reflux, heating, ultrasonic radiation, or supercritical extraction can be applied. In the case of a fractionated extract, the extract is suspended in a specific solvent and then mixed with a solvent of different polarity to form a fraction obtained by adsorbing the crude extract onto a column filled with silica gel and then mixed with a hydrophobic solvent, a hydrophilic solvent, or a mixture of these. It is meant to include fractions obtained using the mobile phase. In addition, the meaning of the extract includes concentrated liquid extract or solid extract from which the extraction solvent has been removed by methods such as freeze-drying, vacuum drying, hot air drying, and spray drying. Preferably, it refers to an extract obtained by using water, ethanol, or a mixed solvent thereof as an extraction solvent, and more preferably, an extract obtained by using a mixed solvent of water and ethanol as an extraction solvent.

In addition, in this specification, “active ingredient” refers to an ingredient that can exhibit the desired activity alone or in combination with a carrier that is not active on its own.

Also, in this specification, “mosquito control” means mosquito repellent or mosquito insecticide. Here, mosquitoes include mosquitoes of the Aedes genus such as Aedes aegypti and Aedes albopictus , red house mosquitoes ( Culex pipiens pallens ), small red house mosquitoes ( Culex tritaeniorhynchus ), and underground house mosquitoes ( Mosquitoes of the Culex genus such as Culex pipiens molestus Forskal , tropical house mosquito ( Culex quinquefasciatus ), and inatomii ( Culex inatomii ), Chinese spotted wing mosquito ( Anopheles sinensis ), Gambian mosquito ( Anopheles gambiae ), It refers to mosquitoes of the genus Anopheles, such as Anopheles minimus and Anopheles stephensi .

In the composition of the present invention, the active ingredient may be included in any amount (effective amount) depending on use, formulation, etc., as long as it can exhibit mosquito control activity, etc., and a typical effective amount is 0.001% by weight based on the total weight of the composition. It will be determined within the range of 20.0% by weight. Here, “effective amount” refers to the amount of the active ingredient included in the composition of the present invention that can exhibit a mosquito control effect. Such effective amounts can be determined experimentally within the scope of the ordinary ability of those skilled in the art.

In addition to the active ingredients, the composition of the present invention may contain any compounds or substances derived from natural products known to have mosquito control activity in the art to enhance mosquito control activity. Such compounds or substances derived from natural products include, for example, 1-(imidazolin-2-yl)amino-1,2-diphenylethane compound (Korean Patent Application No. 2007-7029894), 1-(1,2-diphenyl- Ethyl)-3-(2-hydroxyethyl)-thiourea compound (Korean Patent Application No. 2007-7029893), N-(4-pyridyl)methylsulfonamide (Korean Patent Application No. 2007-7023622), 2 -Cyano-3-(halo)alkoxy-benzenesulfonamide (Korean Patent Application No. 2007-7014451), hydrazide compound (Korean Patent Application No. 2007-7012272), 6-(2-chloro-5-halophenyl ) -Triazolopyrimidine (Korean Patent Application No. 2006-7014704), spiropiperidine derivative (Korean Patent Application No. 2006-7011568), nitrile compound (Korean Patent Application No. 2006-7011448), N-arylhydrazine Derivatives (Korean Patent Application No. 2006-7010797), etc. may be mentioned, and DEET, Achyranthes bidentata extract, Cyanotis arachnoidea extract, neem oil, and bay ( Pimenta ) as exemplified above. racemosa ) extract, clove oil, Hwangbyeol tree extract, citronella essential oil, etc.

In general, vegetable oils are known to have pest repellent and insecticidal activity (Biores. Technol. 99:2507-2515, 2008), and in addition to the oils exemplified above, any plant oil may be used according to the present invention as long as it has pest control activity. It may be included in the composition together with the active ingredient.

The composition of the present invention can be prepared in any dosage form according to the application method by a conventional method known in the art, including a pharmaceutically acceptable carrier in addition to the active ingredient. Specifically, it can be manufactured in formulations such as oil, powder, suspension, fumigant, electric mat, ointment, cream, lotion, gel, external solution, pasta agent, linement agent, soap, spray, etc. Here, “pharmaceutically acceptable” means that it does not inhibit the activity of the active ingredient and does not have any toxicity beyond that acceptable to the human body. Regarding pharmaceutically acceptable carriers, reference may be made to Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, PA, 1995, which is considered a part of this specification.

Carriers that can be used include solid carriers, liquid carriers, gaseous carriers, surfactants, dispersants, etc. Examples of solid carriers include talc, bentonite, clay, kaolin, diatomaceous earth, vermiculite, calcium carbonate, etc., and liquid carriers. Examples include methanol, n-hexanol, ethylene glycol, acetone, methyl ethyl ketone, cyclohexanone, n-hexane, kerosene, kerosene, toluene, xylene, methylnaphthalene, diethyl ether, dioxane, tetrahydrofuran, and ethyl acetate. , acetonitrile, isobutyronitrile, dimethylformamide, dimethylacetamide, soybean oil, cottonseed oil, dimethyl sulfoxide, water, etc., and examples of gaseous carriers include LPG, air, nitrogen, carbon dioxide, dimethyl ether, etc. You can.

The pest control composition of the present invention may also include one or more auxiliaries such as wetting agents, dispersants, surfactants, antifreeze agents, thickeners, preservatives, spreading agents, etc.

These auxiliaries can be used by selecting an appropriate one from among those known in the art. For example, alkylnaphthalene sulfonate (particularly diisopropylnaphthalenesulfonate or diisobutylnaphthalenesulfonate) can be used as a wetting agent, and a nonionic auxiliary agent can be used as a dispersant. Dispersants (ethylene oxide/propylene oxide block polymer, alkylphenol polyglycol ether, tristyrylphenol polyglycol, etc.), anionic dispersants (lignin sulfonic acid, naphthalene sulfonic acid, phenol sulfonic acid, dibutylnaphthalene sulfonic acid, alkylaryl sulfonate, alkyl alcohol) pate, alkyl sulfonate, etc.) or mixtures thereof can be used, and as surfactants, alkyl benzene sulfonate, alkyl naphthalene sulfonate, dialkyl sulfosuccinate, lignin sulfonate, alkyl naphthalene sulfonate formalin condensate, polyoxy Sodium or calcium salts of sulfonates such as alkylene alkylphenylsulfonate, alkyl sulfates, polyoxyalkylene alkyl sulfates, sodium or calcium salts of sulfates such as polyoxyalkylene alkylphenylsulfates, naphthalenesulfosuccinate, Anionic surfactants such as polyoxyalkylene succinate, sodium or calcium salts of succinate, ethoxylated alkyl ethers, polyoxyalkylene alkyl phenyl polymers, polyalcohols, etc. can be used, and methanol and ethanol as antifreeze agents. , isopropanol, butanol, glycol, glycerin, diethylene glycol, etc. can be used. As thickeners, cellulose derivatives, polyacrylic acid derivatives, xanthan, modified clay, etc. can be used. Preservatives include dichlorophen, isothiazolene, benzyl alcohol, etc. can be used, and the electrodeposition agent can be polyvinyl alcohol, polyvinylpyrrolidone, polyacrylate, polymethacrylate, polybutene, polyisobutylene, polystyrene, polyethyleneamine, polyethyleneamide, polyethyleneimine, polyether, etc. You can.

In another aspect, the present invention relates to a method for controlling mosquitoes using the mosquito control composition of the present invention as described above.

The mosquito control method of the present invention includes the step of contacting the mosquito control composition of the present invention as described above with mosquitoes.

The step of bringing the mosquito control composition into contact with pests can be accomplished by spraying or spraying the mosquito control composition of the present invention at a place or location where mosquitoes live or are distributed, or by applying it to human skin, etc. that mosquitoes approach.

As described above, according to the present invention, it is possible to provide a composition and method for controlling mosquitoes using a cucumber extract. The composition for mosquito control of the present invention can be used for mosquito control by spraying or spraying on a place or location where mosquitoes live or are distributed, or by applying it to human skin, etc. that mosquitoes approach.

Figure 1 shows the results of an experiment showing the mosquito insecticidal activity of the cucumber extract.

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. However, the scope of the present invention is not limited to these examples.

<Example> Preparation of Five Herbs Extract

1L of fermented alcohol (95% ethanol) was added to 200g of dried root powder of Lindera strychnifolia and soaked at room temperature for 5 days for extraction, then filtered with Whatman filter paper No. 2 to obtain a brown extraction solution.

< Experiment example > Mosquito killing efficacy experiment

1. Experimental mosquito

Aedes albopictus and Culex pipiens used in the experiment were purchased from the Korea Centers for Disease Control and Prevention and raised under conditions of 26 ± 1°C and 60 ± 5% relative humidity without exposure to pesticides. Larvae were reared in plastic containers (24 × 35 × 5 cm) containing 0.5 g of sterilized feed (40 mesh chick powder feed: yeast, 1:1 weight ratio). Adult worms were supplied with a 10% sugar solution and sucked into live mice for egg laying.

The mosquitoes used in the test were of the same age group, 3 to 4 days after emergence from pupae. The test mosquitoes were fed a sugar solution instead of blood before use, and were starved for about 12 hours before the start of the test.

2. Insecticide effect test

2.1. Exam conditions

The insecticidal effect was investigated according to the Bottle Bioassay of the World Health Organization, Brogdon and McAllister (1998), and the U.S. Centers for Disease Control.

2.2. Test Methods

500 μL of liquid test substance was treated in a 500 mL glass bottle. The test substance was applied to the inside of the bottle by shaking and rolling it well so that the treatment solution was evenly applied to the wall. After drying in a hood for 2 hours to completely volatilize the solvent, 10 female adult mosquitoes were added. All experiments were performed in 3 repetitions. The control group was a test group that was not treated with the test substance (treated only with ethanol).

2.3. Results evaluation

Mosquitoes in the treated and untreated control groups were left under conditions of 12:12 hours of light and dark, 26 ± 1°C, and 60 ± 5% relative humidity, and the mortality rate was examined 24 hours after treatment.

3. Result - insecticidal effect

The mortality rate and the average value of the results of three experiments for female adult Aedes mosquitoes and red house mosquitoes of the test substances are shown in Table 1 and Figure 1 below.

Claims (6)

A composition for controlling mosquitoes containing a water and ethanol mixed solvent extract of the roots of the Chinese herbal medicine as an active ingredient,
A composition in which the mosquito is Aedes albopictus .
delete According to paragraph 1,
A composition in which the extract is a 95% ethanol extract of the root of Culinac chinensis.
delete delete A method for controlling Aedes albopictus, comprising the step of contacting the composition of claim 1 or 3 with Aedes albopictus .