KR102297598B1 - Composition and Method for Controling Mosquito Using an Extract of Calendula arvensis - Google Patents

Abstract

The present invention discloses a composition for controlling mosquitoes using a marigold extract having an insecticidal effect on mosquitoes and a method for controlling the same.

Description

Composition and Method for Controling Mosquito Using an Extract of Calendula arvensis

The present invention relates to a composition for controlling mosquitoes using an extract of marigold ( Calendula arvensis ) and a method for controlling the same.

Mosquitoes, which cause great restrictions on sleep disturbances and nighttime activities in summer, are representative pests that transmit diseases to humans. About 3,500 species are known on Earth, and 55 species in 9 genera are known in Korea. Mosquitoes generally engage in blood-sucking activities to obtain animal protein in order to mature female adult eggs after mating, and secrete saliva containing pathogens or viruses at the blood-sucking site. Through this saliva, mosquitoes transmit diseases such as malaria, encephalitis, yellow fever, dengue fever, and filariasis (Science, 257:37-38, 1992). ), especially Japanese encephalitis, which occurs frequently in Korea, has a high mortality rate and is known to leave severe sequelae such as mental retardation even if treated.

Organophosphorus insecticides and pyrethroid insecticides have been used to control mosquitoes, but the most widely used material is diethyltoluamide (DEET). DEET was first developed by the US military in 1946 and has been used extensively to control various pests such as mosquitoes, flies, fleas, and ticks until now (Int. J. Pharm. 163:167-176, 1998). However, due to its high skin penetration, it can cause transdermal toxicity (Drug Metab. Dispos. 22:106-112, 1994) and cause diseases such as encephalopathy, acute manic psychosis, and cardiovascular disease. (Am. Mosq. Control Assoc. 11: 274-278, 1995). Therefore, its use is restricted for children, pregnant women, hypotensive patients, people with sensitive skin, etc. In particular, in the United States, it is regulated not to manufacture mosquito repellents containing DEET at a concentration of 20% or more.

For these reasons, recent efforts have been made to develop mosquito repellents using natural products with relatively few side effects.

Achyranthes bidentata extract, Cyanotis arachnoidea extract, etc. (Korean Patent No. 10-1778051), neem oil, etc. (Korea Patent Publication No. 10-2017-0049822), Pimenta racemosa ) extracts (Korean Patent Publication No. 10-2004-0070903), Clove Oil, etc. (Korean Patent Publication No. 10-2012-0014536), Hwangbyeok extract, etc. (Korean Patent No. 10-1470477), Citronella essential oil, etc. (Korean Patent Laid-Open Patent Publication No. 10-2014-0042008) has been proposed as a mosquito control agent derived from a natural product.

The present invention also discloses a marigold extract having control activity against mosquitoes.

An object of the present invention is to provide a composition for controlling mosquitoes using a marigold extract.

Another object of the present invention is to provide a method for controlling mosquitoes using a marigold extract.

Other objects or specific objects of the present invention will be set forth below.

The present invention has been completed by confirming the insecticidal effect of marigold extract against Aedes Aedes mosquito, as confirmed in the Examples and Experimental Examples below.

Therefore, the composition for controlling mosquitoes of the present invention is characterized in that it contains a marigold extract as an active ingredient.

In the present specification, as used herein, the term "marigold extract" refers to the extraction target of marigold leaves, stems, above-ground parts, rhizomes, roots, underground parts, or mixtures thereof with water, lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.) , methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or these means an extract obtained by leaching using a mixed solvent of In consideration of this, any method such as chilling, reflux, warming, ultrasonic radiation, and supercritical extraction may be applied. In the case of a fractionated extract, a fraction obtained by suspending the extract in a specific solvent and mixing and standing still with a solvent having a different polarity, and adsorbing the crude extract to a column filled with silica gel, etc. It is meant to include the fraction obtained as a mobile phase. In addition, the meaning of the extract includes a concentrated liquid extract or solid extract from which the extraction solvent is removed by freeze drying, vacuum drying, hot air drying, spray drying, or the like. Preferably, it means an extract obtained by using water, ethanol, or a mixed solvent thereof as an extraction solvent, and more preferably an extract obtained by using a mixed solvent of water and ethanol as an extraction solvent.

In addition, in the present specification, the term "active ingredient" means a component that can exhibit the desired activity alone or can exhibit activity together with a carrier that does not have the activity itself.

Also in this specification, "mosquito control" means mosquito repellent or mosquito repellent. Here, the mosquito is preferably a mosquito of the genus Aedes.

In the composition of the present invention, the active ingredient may be included in any amount (effective amount) depending on the use, formulation, etc. as long as it can exhibit mosquito control activity, etc., and a typical effective amount is 0.001% by weight based on the total weight of the composition. to 20.0% by weight. Here, "effective amount" refers to the amount of the active ingredient included in the composition of the present invention, which can exhibit a mosquito control effect. Such effective amounts can be determined empirically within the ordinary ability of one of ordinary skill in the art.

In addition to the active ingredient, the composition of the present invention may include any compound or natural product-derived material known to have mosquito control activity in the art to enhance the mosquito control activity. Such compounds or substances derived from natural products are, for example, 1-(imidazolin-2-yl)amino-1,2-diphenylethane compound (Korean Patent Application No. 2007-7029894), 1-(1,2-diphenyl- Ethyl)-3-(2-hydroxyethyl)-thiourea compound (Korean Patent Application No. 2007-7029893), N-(4-pyridyl)methylsulfonamide (Korean Patent Application No. 2007-7023622), 2 -Cyano-3-(halo)alkoxy-benzenesulfonamide (Korean Patent Application No. 2007-7014451), hydrazide compound (Korean Patent Application No. 2007-7012272), 6-(2-chloro-5-halophenyl )-triazolopyrimidine (Korean Patent Application No. 2006-7014704), spiropiperidine derivatives (Korean Patent Application No. 2006-7011568), nitrile compounds (Korean Patent Application No. 2006-7011448), N-arylhydrazine derivatives (Korean Patent Application No. 2006-7010797), and the like, and DEET as previously exemplified, Achyranthes bidentata extract, Cyanotis arachnoidea extract, neem oil, Pimenta racemosa ) extract, clove oil, chrysanthemum extract, and citronella essential oil.

In general, vegetable oils are known to have pest repelling activity and insecticidal activity (Biores. Technol. 99:2507-2515, 2008), and any vegetable oils other than the oils exemplified above may also be used as long as they have pest control activity. It may be included in the composition together with the active ingredient.

The composition of the present invention can be prepared in any dosage form according to the application method by a conventional method known in the art, including a pharmaceutically acceptable carrier in addition to the active ingredient. Specifically, it may be prepared in the form of oil, powder, suspension, fumigant, electric mat, ointment, cream, lotion, gel, external solution, pasta, liniment, soap, spray, and the like. Here, "pharmaceutically acceptable" means that it does not inhibit the activity of an active ingredient and does not have more toxicity than can be adapted to the human body. Regarding pharmaceutically acceptable carriers, reference may be made to Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, PA, 1995, which is incorporated herein by reference.

Carriers that can be used include solid carriers, liquid carriers, gaseous carriers, surfactants,

and dispersants. Examples of the solid carrier include talc, bentonite, clay, kaolin, diatomaceous earth, vermiculite, calcium carbonate, and the like. As the liquid carrier, methanol, n-hexanol, ethylene glycol, acetone, methyl ethyl ketone, Cyclohexanone, n-hexane, kerosene, kerosene, toluene, xylene, methylnaphthalene, diethyl ether, dioxane, tetrahydrofuran, ethyl acetate, acetonitrile, isobutyronitrile, dimethylformamide, dimethylacetamide, Soybean oil, cottonseed oil, dimethyl sulfoxide, water, etc. may be exemplified, and as the gaseous carrier, LPG, air, nitrogen, carbon dioxide, dimethyl ether, and the like may be exemplified.

The control agent composition of the present invention may also include one or more adjuvants such as wetting agents, dispersing agents, surfactants, antifreeze agents, thickening agents, preservatives, spreading agents, and the like.

Such adjuvants may be appropriately selected from those known in the art. For example, alkylnaphthalene sulfonate (especially diisopropylnaphthalenesulfonate or diisobutylnaphthalenesulfonate) may be used as a wetting agent, and a nonionic dispersing agent may be used. Dispersing agent (ethylene oxide/propylene oxide block polymer, alkylphenol polyglycol ether, tristyrylphenol polyglycol, etc.), anionic dispersing agent (ligninsulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, dibutylnaphthalenesulfonic acid, alkylarylsulfonate, alkyl sulfonate) paint, alkylsulfonate, etc.) or mixtures thereof, and as the surfactant, alkylbenzenesulfonate, alkylnaphthalenesulfonate, dialkylsulfosuccinate, ligninsulfonate, alkylnaphthalenesulfonate formalin condensate, polyoxy sodium or calcium salts of sulfonates such as alkylenealkylphenylsulfonate, sodium or calcium salts of sulfates such as alkylsulfate, polyoxyalkylenealkylsulfate, polyoxyalkylenealkylphenylsulfate, naphthalenesulfosuccinate; Anionic surfactants such as sodium or calcium salts of succinate such as polyoxyalkylene succinate, ethoxylated alkyl ethers, polyoxyalkylene alkylphenyl polymers, polyalcohols, etc. can be used, and as antifreeze agents, methanol, ethanol , isopropanol, butanol, glycol, glycerin, diethylene glycol, etc. can be used. As a thickener, a cellulose derivative, a polyacrylic acid derivative, xanthan, modified clay, etc. can be used. As a preservative, dichlorophen, isothiazolene, benzyl alcohol, etc. As the electrodeposition agent, polyvinyl alcohol, polyvinylpyrrolidone, polyacrylate, polymethacrylate, polybutene, polyisobutylene, polystyrene, polyethyleneamine, polyethyleneamide, polyethyleneimine, polyether, etc. can be used. can

In another aspect, the present invention relates to a method for controlling mosquitoes using the composition for controlling mosquitoes of the present invention as described above.

The mosquito control method of the present invention comprises the step of contacting the mosquito control composition of the present invention as described above with mosquitoes.

The step of contacting the composition for controlling mosquitoes with pests may be accomplished by spraying or spraying the composition for controlling mosquitoes of the present invention at a place or location where mosquitoes inhabit or distribute, or by applying the composition to human skin where mosquitoes approach.

As described above, according to the present invention, it is possible to provide a composition for controlling mosquitoes using a marigold extract and a method therefor. The composition for controlling mosquitoes of the present invention may be sprayed or sprayed on a place or location where mosquitoes live or are distributed for mosquito control, or applied to human skin where mosquitoes approach.

1 is an experimental result showing the mosquito insecticidal activity of marigold extract.

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. However, the scope of the present invention is not limited to these examples.

< Example > Preparation of marigold extract

After adding 10 times the weight of methanol to the dried marigold powder of Calendula arvensis , leaching at room temperature for 24 hours, filtering with Whatman paper filter paper No. ) was obtained.

< Experimental example > Mosquito insecticidal efficacy test

1. Experimental mosquito

Aedes albopictus used in the experiment was distributed from the Korea Centers for Disease Control and Prevention and bred under conditions of 26±1℃ and 60±5% relative humidity without exposure to pesticides. Larvae were reared in a plastic container (24 × 35 × 5 cm) containing 0.5 g of sterilized feed (40 mesh chick powder feed: yeast, 1:1 weight ratio). Adults were supplied with 10% sugar solution and sucked blood to live mice for spawning.

Mosquitoes used in the test were those of the same age, 3 to 4 days after emergence from the pupa, and the test mosquitoes were supplied with a sugar solution instead of blood until use, and were fasted for about 12 hours before the start of the test.

2. Insecticide effect test

2.1. Exam conditions

The insecticidal effect was investigated according to the Bottle Bioassay of the World Health Organization, Brogdon and McAllister (1998) and the U.S. Centers for Disease Control.

2.2. Test Methods

An appropriate amount of the test substance was dissolved in 0.5 mL ethanol, and the test substance solution was treated in a 500 mL glass bottle for each dose. The chemical solution was applied to the inside of the bottle by shaking it well and rolling it so that the treatment solution could be uniformly applied to the wall. After drying in the hood for about 30 minutes to evaporate the solvent, 10 female adult mosquitoes were put in each. All experiments were performed in 3 replicates. The control group was a test group that was not treated with the test substance (treated only with ethanol).

2.3. result evaluation

Mosquitoes in treated and untreated controls were left under light and dark conditions for 12:12 hours, 26 ± 1°C, and 60 ± 5% relative humidity, and the insecticidal rate was investigated 24 hours after treatment.

3. Results - insecticidal effect

The insecticidal rate by dose for the test substance against adult Aedes Aedes mosquitoes is shown in Table 1 and FIG. 1 below, and the half-lethal concentration is shown in Table 2 below.

Claims (4)

Marigold ( Calendula arvensis ) Aedes albopictus ( Aedes albopictus ) Controlling composition comprising an extract of the whole herb as an active ingredient.
delete According to claim 1,
The composition is characterized in that the extract is a methanol extract.
A method for controlling Aedes Aedes Aedes mosquito comprising the step of contacting the composition of claim 1 or 3 with Aedes Aedes aegypti.

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