KR20220127192A - Composition and Method for Controling Mosquito Using an Extract of Myristica fragrans - Google Patents

Abstract

The present invention discloses a composition for controlling mosquitoes and a method for controlling mosquitoes using a Myristica fragrans extract having a control effect against mosquitoes. Examples of gaseous carriers that can be used in the composition of the present invention include air, nitrogen, carbon dioxide, dimethyl ether, butane, propane, halogenated hydrocarbons, and the like. The composition for controlling mosquitoes of the present invention can be atomized or sprayed on places or locations where mosquitoes live or are distributed for mosquito control, or can be applied to human skin to which mosquitoes approach.

Description

Composition and Method for Controling Mosquito Using an Extract of Myristica fragrans

The present invention relates to a composition for controlling mosquitoes using an extract of nutmeg ( Myristica fragrans ) and a method for controlling the same.

Mosquitoes, which cause great restrictions on sleep disturbances and nighttime activities in summer, are representative pests that transmit diseases to humans. About 3,500 species are known on earth, and 55 species from 9 genera are known in Korea. Mosquitoes generally engage in blood-sucking activities to obtain animal protein in order to mature female adult eggs after mating, and secrete saliva containing pathogens or viruses at the blood-sucking site. Through this saliva, mosquitoes transmit diseases such as malaria, encephalitis, yellow fever, dengue fever, and filariasis (Science, 257:37-38, 1992). ), especially Japanese encephalitis, which occurs frequently in Korea, has a high mortality rate and is known to leave severe sequelae such as mental retardation even if treated.

Organophosphorus insecticides and pyrethroid insecticides have been used to control mosquitoes, but the most widely used material is diethyltoluamide (DEET). DEET was first developed by the US military in 1946 and has been used extensively to control various pests such as mosquitoes, flies, fleas, and ticks until now (Int. J. Pharm. 163:167-176, 1998). However, due to its high skin penetration, it can cause transdermal toxicity (Drug Metab. Dispos. 22:106-112, 1994) and cause diseases such as encephalopathy, acute manic psychosis, and circulatory disease (cardiovascular). (Am. Mosq. Control Assoc. 11: 274-278, 1995). Therefore, its use is restricted for children, pregnant women, hypotensive patients, people with sensitive skin, etc. In particular, in the United States, it is regulated not to manufacture mosquito repellents containing DEET at a concentration of 20% or more.

For these reasons, recent efforts have been made to develop mosquito control agents using natural products with relatively few side effects, such as Achyranthes bidentata extract, Cyanotis arachnoidea extract, etc. (Korean Patent No. 10-1778051), Neem oil, etc. (Korean Patent Publication No. 10-2017-0049822), Bay ( Pimenta racemosa ) extract, etc. (Korean Patent Publication No. 10-2004-0070903), Clove Oil, etc. (Korean Patent Publication) No. 10-2012-0014536), Hwangbyeok tree extract, etc. (Korean Patent No. 10-1470477), Citronella essential oil, etc. (Korean Patent Publication No. 10-2014-0042008) are natural products-derived mosquito control agents. has been proposed.

The present invention also discloses a nutmeg extract having control activity against mosquitoes.

An object of the present invention is to provide a composition for controlling mosquitoes using a nutmeg extract.

Another object of the present invention is to provide a method for controlling mosquitoes using a nutmeg extract.

Other objects or specific objects of the present invention will be set forth below.

The present invention has been completed by confirming the insecticidal effect of the nutmeg extract against Aedes Aedes aegypti and Aedes aphids, as confirmed in the Examples and Experimental Examples below.

Therefore, the composition for controlling mosquitoes of the present invention is characterized in that it contains nutmeg extract as an active ingredient.

As used herein, the term "nutmeg extract" refers to the extraction of nutmeg leaves, stems, above-ground parts, rhizomes, roots, underground parts, or mixtures thereof with water, lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.), methylene chloride, Ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof It refers to an extract obtained by leaching using a supercritical extraction solvent such as carbon dioxide, pentane, or a fraction obtained by fractionating the extract, and the extraction method is cooling, Any method such as reflux, warming, ultrasonic radiation, or supercritical extraction may be applied. In the case of a fractionated extract, it is meant to include a fraction obtained by suspending the extract in a specific solvent and mixing and standing still with a solvent having a different polarity, and a fraction obtained by column chromatography. In addition, the meaning of the extract includes a concentrated liquid extract or solid extract from which the extraction solvent is removed by freeze drying, vacuum drying, hot air drying, spray drying, or the like. Preferably, it refers to an extract obtained using water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof as an extraction solvent, particularly an extract obtained using methanol.

In addition, in the present specification, the term "active ingredient" means a component that can exhibit the desired activity alone, or can exhibit activity together with a carrier that has no activity by itself.

Also in this specification, "mosquito control" means mosquito repellent or mosquito repellent. Here, the mosquitoes are Aedes aegypti, Aedes albopictus , and other Aedes genus mosquitoes, Culex pipiens pallens , Culex tritaeniorhynchus , and Aedes mosquito ( Culex tritaeniorhynchus ). Culex pipiens molestus Forskal ), tropical house mosquito ( Culex quinquefasciatus ), mosquitoes of the genus Culex , such as Culex inatomii , Chinese spotted wing mosquito ( Anopheles sinensis ), Gambian mosquito ( Anopheles gambiae ), It refers to mosquitoes of the Anopheles genus, such as Anopheles minimus and Anopheles stephensi .

In the composition of the present invention, the active ingredient may be included in any amount (effective amount) depending on the use, formulation, etc. as long as it can exhibit mosquito control activity, etc., and a typical effective amount is 0.001% by weight based on the total weight of the composition. to 20.0% by weight. Here, "effective amount" refers to the amount of the active ingredient included in the composition of the present invention, which can exhibit a mosquito control effect. Such effective amounts can be determined empirically within the ordinary ability of one of ordinary skill in the art.

In addition to the active ingredient, the composition of the present invention may include any compound or natural product-derived material known to have mosquito control activity in the art to enhance the mosquito control activity. Such compounds or substances derived from natural products are, for example, 1-(imidazolin-2-yl)amino-1,2-diphenylethane compound (Korean Patent Application No. 2007-7029894), 1-(1,2-diphenyl- Ethyl)-3-(2-hydroxyethyl)-thiourea compound (Korean Patent Application No. 2007-7029893), N-(4-pyridyl)methylsulfonamide (Korean Patent Application No. 2007-7023622), 2 -Cyano-3-(halo)alkoxy-benzenesulfonamide (Korean Patent Application No. 2007-7014451), hydrazide compound (Korean Patent Application No. 2007-7012272), 6-(2-chloro-5-halophenyl )-triazolopyrimidine (Korean Patent Application No. 2006-7014704), spiropiperidine derivatives (Korean Patent Application No. 2006-7011568), nitrile compounds (Korean Patent Application No. 2006-7011448), N-arylhydrazine derivatives (Korea Patent Application No. 2006-7010797), and the like, and DEET as previously exemplified, Achyranthes bidentata extract, Cyanotis arachnoidea extract, neem oil, Pimenta racemosa ) extract, clove oil, chrysanthemum extract, and citronella essential oil.

In general, vegetable oils are known to have pest repelling activity and insecticidal activity (Biores. Technol. 99:2507-2515, 2008), and any vegetable oils other than the oils exemplified above may be used as long as they have pest control activity. It may be included in the composition together with the active ingredient.

The composition of the present invention may be prepared in any dosage form according to the type of application by a conventional method known in the art, including a solid carrier, a liquid carrier, or a gaseous carrier acceptable in the related art in addition to the active ingredient. Specifically, liquid, emulsion, wettable powder, water soluble powder, water dispersible granule, water soluble granule, suspension, emulsion, powder (dustable powder), granule (granule), tablet (tablet), emulsifiable gel (emulsifiable gel), spray, fumigant, aerosol, smoke, etc. may be prepared in any formulation.

As the solid carrier that can be used in the composition of the present invention, natural minerals, calcined natural materials, inorganic salts, sugars, organic materials, powders of plants, etc. can be used, and specifically, quartz, calcite, sepiolite, dolomite, chalk, kaolinite, Phylophyllite, Cellicite, Halosite, Metahalosite, Wood Grinding Clay, Slotting Clay, Ceramic Stone, Declite, Allopen, Shiras, Mica, Talc, Bentonite, Activated Clay, Acid Clay, Pumice Stone, Attapulgite , zeolite, diatomaceous earth calcined clay, perlite, shiras balloon, vermiculite, atapulgus clay, calcined diatomaceous earth, magnesium carbonate, calcium carbonate, sodium carbonate, sodium hydrogen carbonate, ammonium sulfate, sodium sulfate, magnesium sulfate, diammonium hydrogen phosphate, ammonium dihydrogen phosphate , potassium chloride, glucose, fructose, sucrose and lactose, starch, powdered cellulose, dextrin, urea, urea derivatives, benzoic acid, benzoate, wood flour, cork flour, cornstarch, walnut husks, tobacco stems, fly ash, white carbon (such as , hydrous synthetic silica, anhydrous synthetic silica, hydrous synthetic silicate, etc.), fertilizer, and the like can be used.

In addition, as the liquid carrier that can be used in the composition of the present invention, aromatic hydrocarbons, aliphatic hydrocarbons, alcohols, ethers, esters, amides, oils and fats can be used, and specifically, xylene, alkylbenzene, phenylxylylethane , alkylnaphthalene, machine oil, normal paraffin, isoparaffin, naphthene, ethanol, isopropanol, cyclohexanol, phenoxyethanol, benzyl alcohol, ethylene glycol, propylene glycol, diethylene glycol, hexylene glycol, polyethylene glycol, polypropylene glycol , propyl cellosolve, butyl cellosolve, phenyl cellosolve, propylene glycol monomethyl ether, propylene glycol monoethyl ether, propylene glycol monopropyl ether, propylene glycol monobutyl ether, propylene glycol, monophenyl ether, acetophenone, Cyclohexanone, γ-butyrolactone, fatty acid methyl ester, succinic acid dialkyl ester, glutamic acid dialkyl ester, adipic acid dialkyl ester and phthalic acid dialkyl ester, N-alkylpyrrolidone, soybean oil, linseed oil, rapeseed oil, palm oil , cottonseed oil, castor oil, dimethyl sulfoxide, water, and the like can be used.

In addition, as the gaseous carrier that can be used in the composition of the present invention, air, nitrogen, carbon dioxide, dimethyl ether, butane, propane, halogenated hydrocarbons and the like can be exemplified.

The composition of the present invention may further include one or more adjuvants, such as surfactants, dispersants, wetting agents, antifreeze agents, thickeners, preservatives, spreading agents, colorants, and stabilizers, if necessary, in addition to the active ingredient and the carrier component.

Any surfactants such as nonionic surfactants, anionic surfactants, and cationic surfactants may be used as the surfactant, and specifically, polyoxyethylene alkyl ether, polyoxyethylene alkylphenyl ether, polyoxyethylene styrylphenyl ether, etc. , polyoxyethylene polyoxypropylene block copolymer, polyoxyethylene fatty acid ester, sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, castor oil ethylene oxide adduct, acetylene glycol, acetylene alcohol, ethylene oxide adduct of acetylene glycol , ethylene oxide adduct of acetylene alcohol, nonionic surfactants such as alkyl glycoside, alkyl sulfate ester salt, alkylbenzenesulfonate, ligninsulfonate, alkylsulfosuccinate, naphthalenesulfonate, alkylnaphthalenesulfonate, naphthalenesulfonate Salt of the formalin condensate of phonic acid, the salt of the formalin condensate of alkylnaphthalenesulfonic acid, polyoxyethylene alkyl ether sulfuric acid or phosphoric acid ester salt, polyoxyethylene alkylphenyl ether sulfuric acid or phosphoric acid ester salt, polyoxyethylene styrylphenyl ether sulfuric acid or phosphoric acid Ester salts, polycarboxylates, polystyrene sulfonates, alkylamine salts, alkylammonium salts, silicone-based surfactants, fluorine-based surfactants, and the like may be used.

As the dispersant, nonionic dispersants (ethylene oxide/propylene oxide block polymer, alkylphenol polyglycol ether, tristyrylphenol polyglycol, etc.), anionic dispersants (lignin sulfonic acid, naphthalene sulfonic acid, phenol sulfonic acid, dibutylnaphthalene sulfonic acid, alkylaryl) sulfonate, alkyl sulfate, alkylsulfonate, etc.) or mixtures thereof, and alkylnaphthalene sulfonate (especially diisopropylnaphthalenesulfonate or diisobutylnaphthalenesulfonate) can be used as a wetting agent, and an antifreeze agent Methanol, ethanol, isopropanol, butanol, glycol, glycerin, diethylene glycol, etc. can be used as the thickener, and as the thickener, cellulose derivatives such as carboxymethyl cellulose, polyacrylic acid derivatives, polyvinylpyrrolidone, carboxyvinyl polymer, high purity bentonite, xanthan Gum, modified clay, xanthan gum, guar gum, diutan gum, starch derivatives, etc. can be used, and dichlorophene, isothiazolene, benzyl alcohol, etc. can be used as preservatives.

In addition, polyvinyl alcohol, polyvinylpyrrolidone, polyacrylate, polymethacrylate, polybutene, polyisobutylene, polystyrene, polyethyleneamine, polyethyleneamide, polyethyleneimine, polyether, etc. can be used as the electrodeposition agent, and a colorant As the inorganic pigments such as iron oxide, titanium oxide, and Prussian blue, organic pigments such as alizarin dye, azo dye, and metal phthalocyanine dye can be used. As the stabilizer, sodium benzoate, potassium sorbate, para-hydroxybenzoic acid can be used. Esters, 1,2-benzothiazolin-3-ones, preservatives such as formalin solutions, cryoprotectants such as polyhydric alcohols such as ethylene glycol, diethylene glycol, propylene glycol, glycerin, starch, alginic acid, mannose, galactose, poly Vinylpyrrolidone, ester gum, cryoprotectant, zeolite, calcium oxide, and drying agents such as magnesium oxide; antioxidants such as phenolic antioxidants, amine antioxidants, sulfur antioxidants, phosphoric acid antioxidants, etc.; Decomposition inhibitors, such as a ultraviolet absorber, etc. can be used.

For more specific information regarding formulation types, carriers, and adjuvants, see "Catalogue of Pesticide Formulation Types and International Coding System" Technical Monograph, 5th Edition by CropLife International (2002), Crop Life International and in Pesticide Specifications, Manual on development and use of FAO and WHO specifications for pesticides, FAO Plant Production and Protection Papers - 173, prepared by the FAO/WHO Joint Meeting on Pesticide Specifications, 2004, ISBN: 9251048576, Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, PA, 1995], literature [Guide to Pesticide Formulation, Japanese Agrochemical Society/Application Research Society, Published by Japan Plant Prevention Association, 1997], etc. can be referred to. These documents, together with other documents cited herein, are considered a part of this specification.

In another aspect, the present invention relates to a method for controlling mosquitoes using the composition for controlling mosquitoes of the present invention as described above.

The mosquito control method of the present invention comprises the step of contacting the mosquito control composition of the present invention as described above with mosquitoes.

The step of bringing the composition for controlling mosquitoes into contact with mosquitoes may be accomplished by spraying or spraying the composition for controlling mosquitoes of the present invention at a place or location where mosquitoes inhabit or distribute, or by applying the composition to human skin where mosquitoes approach.

As described above, according to the present invention, it is possible to provide a composition for controlling mosquitoes using a nutmeg extract and a method therefor. The composition for controlling mosquitoes of the present invention may be sprayed or sprayed on a place or location where mosquitoes live or are distributed for mosquito control, or applied to human skin where mosquitoes approach and can be used.

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. However, the scope of the present invention is not limited to these examples.

< Example > Preparation of Nutmeg Extract

After adding 10 times the weight of methanol to the dried nutmeg ( Myristica fragrans ) fruit powder, leaching at room temperature for 24 hours, filtration with Whatman paper filter paper No. 2, concentration under reduced pressure and freeze-drying to obtain a powdery extract.

< Experimental example > Mosquito Insecticide Efficacy Test

1. Experimental mosquito

Aedes albopictus and Culex pipiens used in the experiment were purchased from the Korea Centers for Disease Control and Prevention and bred under conditions of 26±1℃ and 60±5% relative humidity without exposure to pesticides. Larvae were reared in a plastic container (24 × 35 × 5 cm) containing 0.5 g of sterilized feed (40 mesh chick powder feed: yeast, 1:1 weight ratio). Adults were supplied with a 10% sugar solution and sucked blood to live mice for spawning.

Mosquitoes used in the test were those of the same age, 3 to 4 days after emergence from the pupa, and the test mosquitoes were supplied with a sugar solution instead of blood until use, and were fasted for 12 hours before the start of the test.

2. Insecticide effect test

2.1. Exam conditions

The insecticidal effect was investigated according to the Bottle Bioassay of the World Health Organization, Brogdon and McAllister (1998) and the U.S. Centers for Disease Control.

2.2. Test Methods

An appropriate amount of the test substance was dissolved in 0.5 mL ethanol, and the test substance solution was treated in a 500 mL glass bottle for each dose. The chemical solution was applied to the inside of the bottle by shaking it well and rolling it so that the treatment solution could be uniformly applied to the wall. After drying in the hood for about 30 minutes to evaporate the solvent, 10 female adult mosquitoes were added each. All experiments were performed in 3 replicates. The control group was a test group that was not treated with the test substance (treated only with ethanol).

2.3. Results evaluation

Mosquitoes in the treated and untreated controls were left under light and dark conditions for 12:12 hours, 26 ± 1°C, and 60 ± 5% relative humidity, and the insecticidal rate was investigated 24 hours after treatment.

3. Results - insecticidal effect

Table 1 below shows the mortality rates for each dose of the test substance against adult Aedes Aedes mosquitoes and Aedes Aedes mosquitoes.

Claims (6)

A composition for controlling mosquitoes comprising nutmeg extract as an active ingredient.
According to claim 1,
The mosquitoes are Aedes ( Aedes ) genus mosquitoes, genus Culex genus Mosquitoes or Aedes mosquitoes (Anopheles) genus Mosquitoes.
According to claim 1,
The mosquitoes are Aedes aegypti (Aedes aegypti), Aedes Aedes mosquito ( Aedes albopictus ), Red house mosquito ( Culex pipiens pallens ), Little red house mosquito ( Culex tritaeniorhynchus ), Underground house mosquito ( Culex pipiens molestus Forskal ), Tropical house mosquito ( Culex quinquefasciatus ), Inadomi mosquito ( Culex inatomii ), Chinese spotted winged mosquito ( Anopheles sinensis ), Gambian mosquito mosquito ( Anopheles gambiae ), Anopheles minimus , or Anopheles stephensi ).
According to claim 1,
The mosquito is Aedes albopictus ( Aedes albopictus ) or red mosquito ( Culex pipiens pallens ) The composition.
According to claim 1,
The extract is water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent extract thereof.
A method for controlling mosquitoes comprising the step of contacting the composition of any one of claims 1 to 5 with mosquitoes.