KR100478135B1 - Insecticidal oil derived from plant and compound - Google Patents

Abstract

The present invention relates to plant essential oils and compounds having insecticidal properties. In particular, the present invention relates to Salvia Officinalis, Pogostemon Patchuli, Citrus Aurantifolia, Citrus Paradish, Salvia Scleria, Origum Bulgari, which can kill stock pests, sanitary pests and agricultural pests. , Citrus Retigulata, Eucalyptus Citridora, Boswellia Carteri, Pipenigrum, Cympogon Martini, Abies Alba, Cambopogon Cilatus, Menta Fulleium, Bettyvera Zyzanoids, Citrus Limonum, Menta Essential oils extracted from one or more plants selected from the group consisting of spicata, capsicum annum bur agulosum, jinger officinalis, citrus aurantium, citrus sinensis, cupresus sempervirens and oxymum basilicum It provides a pesticidal composition comprising a.

Description

Insecticidal plant essential oils and compounds {INSECTICIDAL OIL DERIVED FROM PLANT AND COMPOUND}

[TECHNICAL FIELD OF THE INVENTION]

The present invention relates to plant essential oils and compounds having insecticidal properties, and more particularly, to plants having excellent insecticidal action against mosquitoes, ants, ticks, wheels and stored pests, and essential oils and insecticidal compounds extracted from the plants. .

[Private Technology]

Plant-derived natural products not only exhibit good insecticidal effects with small amounts of use, but are also easily biodegradable and have low maternal toxicity and low risk to the environment. Due to the advantages of these natural products, research is being actively conducted to use natural products as a substitute for controlling insects. In particular, natural essential oils derived from plants have traditionally been used as a means to control stored pests and to combat domestic pests. Recently, they are also used as cosmetics and herbal medicines are used to treat diseases by using aromatherapy. It is also applied to treatment.

There is a great deal of research on the pesticidal activity of essential oils against stock pests, and plant essential oils have been found to show great pesticidal activity against each stock pest. Typically, the major components of most natural essential oils, cinnamic aldehyde, alpha-pinene, anetol, cloves and fennel, have insecticidal activity against Tribolium castaneum and Sitophilus zeamais . Ho, SH, Cheng, LPL, Sim, KY, Tan, HTW 1994.Postharvest Biology and Technology. 4: 179-183; Ho, SH, Ma, Y., Goh, PM, Sim, KY 1995. Postharvest Biology and Technology . 6: 341-347; Ho, SH , Ma, Y., Huang, Y. 1997. International Pest Control 39 (2):.. 50-51 .; Huang, Y., Ho, SH 1998. Journal of Stored Product Research.34 : 11-17 .; Huang, Y., Hee, SK, Ho, SH 1998.International Pest Control.40 (1): 18-20)

Natural essential oils include having insecticidal activity against agricultural pests queue minyu (Cuminum cyminum), anise oil (Pimpinella ansium), oregano oil (Origanum syriacum var. Bevanii) and eucalyptus Tooth Oil (Eucalyptus camaldulensis) is, and these cotton aphid (Aphis gossypii ), Tetranychus cinnabarinus and two greenhouse pests (Tuni, I., Sahinkaya, S. 1998. Entomological Experiments and Application . 86: 183-187). It is toxic to mite ( Tetranychus urticae ) and housefly ( Musca domestica ), and monoterpinoid compounds show their inhibitory activity against Ostrinia nubilalis (Lee, S., Tsao, R., Peterson). , CJ, Coats, JR 1997. Journal of Economic Entomology . 90: 883-892)

The mechanism of action of insecticidal plant essential oils is not the same, but the knockdown and lethal effects are due to neurotoxicity (Ngoh, SP, Hoo, L., Pang, FY, Huang, Y., Kim, MR). , Ho, SH 1998. Pescicide Science . 54: 261-268; Rice, PJ, Coats, JR 1994. Pesticide Science . 41: 195-202; Coats, JR, Karr, LL, Drewes, CD 1991. A American Chemistry Society Symposium Series No. 449. pp. 306-316) Monoterpinoids isolated from plant essential oils are known to be competitive inhibitors of acetylcholinesterase in vitro (Grundy, DL, Still, CC). Inhibition of acetylcholinesterases by pulegone-1,2-epoxide.Pesticide Biochemistry and Physiology. 23: 383-388; Miyazawa, M., Watanabe, H., Kameoka, H. 1997.Inhibition of acetylcholiesterases activity by monoterpenoids with a p -menthane skeleton.Journal of Agricultural and Food Chemistry. 45: 677-679). It does not match.

In addition, natural essential oils and compounds having insecticidal activity have a large difference in insecticidal activity depending on the type of insect, and development of an insecticide that optimally works for each insect is required.

An object of the present invention is to provide a plant having insecticidal properties.

It is another object of the present invention to provide a plant essential oil having insecticidal properties against hygienic pests.

It is another object of the present invention to provide a plant essential oil having insecticidal properties against stock pests.

It is another object of the present invention to provide a plant essential oil having insecticidal properties against agricultural pests.

It is another object of the present invention to provide a plant-derived compound having insecticidal properties against pests.

It is another object of the present invention to provide a pesticide composition comprising an insecticidal plant essential oil.

It is another object of the present invention to provide a pesticide composition comprising a pesticidal plant-derived compound.

In order to achieve the above object, the present invention provides Salvia officinalis , Pogostemon patchouli , Citrus aurantifolia , Citrus paradisi , and Salvia . Salvia sclerea , Origanum vulgare , Citrus reticulata , Eucalyptus citriodora , Boswellia carterii , Piper nigrum ), Cymbopogon martini , Abies alba , Cymbopogon citratus , Mentha pulegium , Vetivera zizanoides , Citrus limonum Citrus limonum , Mentha spicata , Capsicum annuum var. Angulosum , Zinger officinalis , Citrus aus Contains essential oils extracted from at least one plant selected from the group consisting of Citrus aurantium , Citrus sinensis , Cupressus sempervirens and Ocimum basilicum It provides an insecticidal composition.

In addition, the present invention is asaron (asarone), cedrol (cedrol), acetyleugenol (acethyleugenol), isoeugenol (isoeugenol), beta-tujaplicine (β-thujaplicine), (+)-Fanchon ((+) -fenchone), (-)-panchone ((-)-fenchone), 3-methoxybenzaldehyde, precocene (7-methoxy-2,2-dimethyl-chromene), acetic acid beta- Naphthyl ester (acetic acid β-naphthyl ester), anisin acid (4-methoxybenzoic acid), carbonyl acetate (bornyl acetate), safrole, alpha-humolene (α-caryophyllene) , Terre blood nolren (terpinolene), allyl anisole (allylanisole), trans-beta-pyrene (trans -β-pinene), beta-propylene karioh ((-) - (trans) -caryphyllene, β-caryophyllene) and 3- Provided is an insecticidal composition comprising at least one selected from the group consisting of 3-carene.

Hereinafter, the present invention will be described in detail.

The inventors of the present invention are Juniperus oxycedrus , Elettaria cardamomum , Juniperus communis , Carum carvi , Tagetes glandulifera , Anthemis nobilis , Coriandrum sativum , Cymbopogon nardus , Salvia officinalis , Pogostemon patchouli , Seed Citrus bergamia , Pelargonium graveolens , Citrus aurantifolia , Citrus paradisi , Salvia sclerea , Pimento Pimento racemosa , Mentha piperita , Origanum vulgare , Myrtus communis , Eugenia karyopilata ( Eu) genia caryophyllata , Eucalyptus globulus , Aniba rosaeodora , Citrus reticulata , Melaleuca tenifolia , Cedus atlanticus , Eucalyptus eucalyptus Eucalyptus citriodora , Artemesia absinthium , Boswellia carterii , Rosmarinus officinalis , Piper nigrum , Cymbopogon martini Cymbopogon martini , Citrus aurantium , Thymus vulgaris , Citrus reticulata , Abies alba , Thymus masticina , Cympogon Sheet Cymbopogon citratus , Mentha pulegium , Vetivera zizanoides , Citrus limonum) , Mentha spicata , Eugenia caryophyllata , and Capsicum annuum var. angulosum), jinggeo operational during day-less (Zinger officinalis), pre seutika Fragrance (Myristica fragrans), Raven dulra hybridizes (Lavendula hybrida), citrus brother is tium (Citrus aurantium), citrus sinen sheath (Citrus sinensis), cue presentation Confirm that Cupressus sempervirens , Santalum album Ocimum basilicum , Cinnamon, Horseradish, Mustard and Ylang Ylang are insecticidal plants, The present invention has been completed.

Plant essential oils were extracted from the plants or purchased essential oils were tested for pesticidal activity. Extraction of plant essential oils can be carried out by conventional methods. Representative extraction methods include distillation using water or steam and compression.

The plant essential oil of the present invention is preferably extracted from flowers, leaves, stems, buds, roots, resins, fruits, seeds, plants and the like. The plant essential oils sold can be purchased and used according to the extraction part of the plant. Conventional extraction sites and sales names for each plant are shown below. However, the plant essential oil of the present invention is not limited to the following.

Essential oils extracted from the leaves are Timus Bulgari's Thyme white, Juniper's Oxededus' Cade, Tazetes Glandolipera's Tagetes, and Cimbopogon Narder's Citronella Java (Citronella). java), Sage of Salvia Offisinalis, Patchouli of Pogostemon Patchouli, Bay of Pimento Racemosa, Oregano of Bulgari, Origano, Migtus Komunis Myrtle, Eucalyptus globulus, Cloveleaf, Eucalyptus globulus, Eucalyptus, Melal Eucatinifolia Tea tree, Eucalyptus Ciriodora Lemon eucalyptus , Petitgrain of Citrus Aurantium, Thyme Red of Timus Bulgari, Marjoram of Timus Masthina, Lemongrass and Mentha of Cympogon Citratus Pennyroyal of Fulleium.

The essential oils from the fruit are Cardamone ceylon of Eletaria Cerdamomum, Juniperberry of Juniper's Komunis, Coriander of Korean Dream Satiboom, and Black Pepper of Pipenigrum. pepper, Pimento berry from Capsicum annum bur Agulosum and Nutmeg from Mystica fragrance.

The essential oil extracted from the seeds is Carum Carby's Caraway.

Essential oils extracted from flowers include Chamomile roman of Anemises Nobilis, Geranium of Pelargonium Graveolens, Clary sage of Salvia Scleria, Peppermint of Menta Piperita (Peppermint), Citrus Retigulata's Tangerine, Rosemary's Rosemary, Rosemary, Menta Spicata's Spearmint, Ravendula Hybrid's Lavender and Oxymum Basilicum's Basil.

Essential oils extracted from the husks are: Bergamot of Citrus bergamia, Lime of Citrus aurantifolia, Grapefruits of Citrus paradish, Mandarin of citrus reticulata, Lemon of citrus limonum (Lemon), Bitter Orange of Citrus Aurantium, Orange of Citrus Synensis and Cypress of Cuprus Sempervirens.

The essential oils extracted from the trees are Rosewood of Aniva Rosodora, Cedarwood of Cedus Atlantica and Sandalwood of Santalum Alboom.

Essential oils extracted from the plant include Wormwood of Aphemium, Artessia, Palmarosa of Cympogon Martini, and Vetiver haiti of Betivera Zijanoides.

The essential oil extracted from the resin is Frankenscence of Boswellia Carteri, and the essential oil extracted from the conifer is the Fui needle of buds and buds of Abies alba, and the Clovebud of Eugenia Caryopilata The essential oil extracted from the root is Ginger of Zinger Offisinalis.

The plant essential oil of the present invention was tested for pesticidal activity according to the pests, and as a result, plant essential oils having excellent insecticidal rate were selected according to the pests. Pests referred to in the present invention include, but are not limited to, hygienic pests, stock pests, agricultural pests, dry pests and forest pests. Representatives of sanitary pests are mosquitoes, flies, wheels, ticks, and ants, and representatives of stored pests are Hwaranggok moths, red bean weevil, rice weevil, lineworm moth, single pointed rice moth, barley moth, false rice steal meal, Kwon Yeon. The worms, blacksmiths, are flatworms, long-headed bald heads, bogeyworms, black bogeyworms and wormwoodworms. Representatives of dry pests are termites, and forest pests include pine needles, white fire moths, tent moths, gypsy moths, cicada moths, scarabs, aphids, mites, pine bark beetles, pine broadleaf bees, pineapple moths and pine needles. Etc. Representative agricultural pests are greenhouse dust , Nilaparvata lugens Stal, Laodelphax striatellus Fallen, white locust , cicada larva and larva .

Essential oils that have pesticidal activity against stored pests include juniperus oxysedrus, eletaria cerdamomum, juniperus komunis, karum carvi, tagetes glandolipera, anthemis nobilis, coriandrum sativaum, cymbo Pogon Narders, Salvia Offisinalis, Pogostemon Patchuli, Sidrus Bergamoia, Pelargonium Graveolens, Citrus Aurantifolia, Citrus Paradish, Salvia Scleria, Pimento Lacemosa , Menta Piperita, Origanum Bulgari, Migtus Komunis, Eugenia Karyopilata, Eucalyptus Globulus, Aniva Rososadora, Citrus Retigulata, Melalyucatenifolia, Cedus Atlantica, Eucalyptus Citridora, Artesia Absintia, Boswellia Carteri, Rosemarinus Orignalis, Fife Nigrum, Cympogon Martini, Citrus Aurantium, Timus Bulgari, Citrus Reticulata, Abies Alba, Timus Mastina, Cympogon Citratus, Menta Pullegium, Betivera Zijanoides, Citrus Limonum, Menta Spicata, Eugenia Caryopilata, Capsicum From the group consisting of Anumber Agulosum, Zinger Officinalis, Mystica Fragrance, Ravendula Hybrida, Citrus Aurantium, Citrus Synensis, Cupresus Sempervirens, Santalum Alboom and Oxymum Basilicum. Essential oils extracted from one or more selected plants are preferred.

More preferably, essential oils having insecticidal activity against Julalac moths are Migtus komunis, eucalyptus globulus, rosemarinus orignalis, timus mastisina, mysticica fragrance and cupreus sempirence. It is extracted from at least one selected plant from the group consisting of. The essential oils that have insecticidal activity against Hwarangok Moth are Juniperus Comunis, Migtus Communis, Eucalyptus Globulus, Boswellia Carteri, Rosemarinus Orignalis, Abies Alba, Timus Mastina, and Mystica Frag. It is extracted from at least one plant selected from the group consisting of Lance and Cupresus Sempervirens. Essential oils that have insecticidal activity against red bean weevil are juniperus oxedrus, eletaria cerdamomum, juniperus komunis, karum carvi, tagetes glandolipera, anthemis nobilis, coriandrum sativaum, cymbopogon Narders, Salvia Offisinalis, Pogostemon Patchuli, Sidrus Bergamoia, Pelargonium Graveolens, Citrus Aurantifolia, Citrus Paradish, Salvia Scleria, Pimento Lacemosa, Menta Piperita, Origanum Bulgari, Myggus Comunis, Eugenia Caryopilata, Eucalyptus Globulus, Aniva Rososadora, Citrus Retigulata, Melalyucatenifolia, Cedus Atlantica, Eucalyptus Citrudora, Arte Messiah Absinium, Boswellia Carteri, Rosemarinus Orignalis, Fife Nigrum, Cympogon Martini, Citrus Aurantium, Moose vulgaris, citrus Retina other Kula, Avi Dar Alba, tea mousse maseuti Sina, pogon Citra crooked tooth, menta Poole declination, Betty Vera prophet Noi Death , Citrus limonum, menta spicata, eugenia caryopilata, capsicum anum bur agulos, zinger officinalis, mysticica fragrance, lavandula hybrida, citrus aurantium, citrus sinensis and cupreus It is extracted from at least one plant selected from the group consisting of Sempervirens. Essential oils with insecticidal activity against rice weevil are juniperus oxysedrus, eletaria cerdamomum, juniperus komunis, karum carvi, tagetes glandolipera, anthemis nobilis, coriandrum sativaum, symbopogon Narders, Salvia Offisinalis, Pogostemon Patchuli, Sidrus Bergamoia, Pelargonium Graveolens, Citrus Aurantifolia, Salvia Scleria, Pimento Lacemosa, Origanum Bulgari, Migter's Komunis, Eugenia Caryopilata, Eucalyptus Globulus, Aniva Rosodora, Citrus Retigulata, Melalyucatinifolia, Eucalyptus Citridora, Artesia Absinium, Boswellia Carteri, Rosemarinus Orignalis, Pipene Nigrum, Cympogon Martini, Citrus Aurantium, Timus Bulgari, Citrus Reticulata, Abies Alba, T Smastisina, Cympogon Citrusus, Menta Fulleum, Citrus Limonum, Menta Spicata, Eugenia Caryopilata, Capsicum Anumber Agulosum, Zinger Officinalis, Mysticica Fragrance, Citrus Aurantium , Citrus sinensis, cupresus Sempervirens, Santalum Alboom and oxymum basilicum.

In addition, essential oils that have insecticidal activity against hygienic pests include juniperus oxysedrus, eletaria cerdamomum, juniperus komunis, karum carvi, anthemis nobilis, coriandrum satiboom, cymbopogon nartus, salvia Officinalis, Pogostemon Patchouli, Sidrus Bergamoia, Pelargonium Graveolens, Citrus Aurantifolia, Citrus Paradish, Salvia Scleria, Pimento Lacemosa, Menta Piperita, Origa Over Bulgari, Migters Comunis, Eugenia Caryopilata, Eucalyptus Globulus, Aniva Rosodora, Citrus Retigulata, Melalyucatinifolia, Eucalyptus Citridora, Artesia Absintia, Boswellia Carteri , Rosemarinus Orignalis, Pipenigrum, Cympogon Martini, Citrus Aurantium, Timus Bulgari, Citrus Reticulata, Vis Alba, Timus Masthina, Cympogon Citruss, Menta Fulgeum, Menta Spicata, Eugenia Caryopilata, Capsicum Anumbur Agulosum, Zinger Opisinalis, Miricica Fragrance, Ravendul Hybrida It is preferred that the extract is from at least one plant selected from the group consisting of citrus sinensis, cupresus sempervirens, Santalum alboom and oxymum basiliccum.

More preferably, essential oils having insecticidal activity against ticks include Timus vulgaris, juniperus oxedrus, carum carby, cymbopogon nartus, pogostemon patchouli, pelargonium gravelens, citrus auran Tifolia, Pimento Racemosa, Menta Piperita, Origanum Bulgari, Eugenia Karyopilata, Simbopogon Martini, Simbopogon Cilatus, Menta Fulleium, Citrus Limonum, Eugenia Caryophylata, Capsicum Anumber It is extracted from at least one selected plant from the group consisting of Agulo island, Myristica fragrance and Santalum Alboom.

Essential oils that have insecticidal activity against mosquitoes include Timus vulgaris, Antemis nobilis, pogostemon patchuli, citrus paradish, migtus komunis, eugenia karyopilata, citrus retigulata, melal yucatenifolia, citrus Reticulata, Abies Alba, Timus Mastina, Cympogon Citratus, Menta Fulgeum, Zinger Officinalis, Mystica Fragrance, Ravendula Hybrida, Citrus Aurantium, Citrus Synensis, Cupre More preferably, it is extracted from at least one plant selected from the group consisting of Sus Sempervirens, Santalum Alboom and Oxymum Basilicum.

Essential oils that have insecticidal activity against the wheel include Timus vulgaris, juniperus oxedrus, eletaria cerdamomum, karum carvi, coriandrum sativaum, salvia opusinalis, citrus aurantifolia, citrus paradi Poetry, Pimento Lasmososa, Menta Piperita, Migtus Komunis, Eucalyptus Globulus, Aniva Rosaodora, Citrus Retigulata, Melalyucatinifolia, Eucalyptus Citridora, Artesia Absinium, Boswellia Carteri, Rosemarinus Orignalis, Fife Nigrum, Citrus Reticulata, Abies Alba, Timus Mastina, Simbopogon Cilatus, Menta Furegium, Menta Spicata, Eugenia Caryophylata, Capsicum Anumbur Agulosum, Zinger Officinalis, Mystica Fragrance, Ravendula Hybrida, Citrus Aurantium, Citrus Synensis, More preferably, it is extracted from at least one plant selected from the group consisting of Cupresuses Sempervirens and Oxymum Basilicum.

Essential oils with insecticidal activity against ants include Timus vulgaris, juniperus oxedrus, eletaria cerdamomum, juniperus komunis, karum carvi, anthemis nobilis, coriandrum sativaboom, cymbopogon nartus, Consisting of Salvia Officinalis, Citrus Bergamoia, Citrus Aurantifolia, Salvia Scleria, Pimento Racemosa, Menta Piperita, Origanum Bulgari, Migters Komunis, Eucalyptus Globulus and Cinnamon It is preferred that the extract is from at least one plant selected from the group.

In addition, essential oils that have insecticidal activity against agricultural pests include juniperus oxysedrus, eletaria cerdamomum, juniperus komunis, karum carvi, tagetes glandolipera, anthemis nobilis, coriandrum sativaum, cymbo Pogon Narders, Salvia Offisinalis, Pogostemon Patchuli, Sidrus Bergamoia, Pelargonium Graveolens, Citrus Aurantifolia, Citrus Paradish, Salvia Scleria, Pimento Lacemosa , Menta Piperita, Origanum Bulgari, Migters Comunis, Eugenia Karyopilata, Eucalyptus Globulus, Aniva Rososadora, Citrus Retigulata, Melalyucatonifolia, Eucalyptus Citridora, Artesia Absinium , Boswellia Carteri, Rosemarinus Orignalis, Pipenigrum, Cymbogongon Martini, Citrus Aurantium, Timus Bulgari , Citrus Reticulata, Abies Alba, Timus Mastina, Cympogon Citratus, Menta Fulleium, Betty Vera Zijanoides , Citrus limonum, menta spicata, eugenia caryopilata, capsicum anum bur agulos, zinger officinalis, mysticica fragrance, lavandula hybrid, citrus aurantium, citrus sinensis, cupreus It is preferably extracted from at least one plant selected from the group consisting of Sempervirens, Santalum Alboom, oxymum basilicum, cinnamon, horseradish, mustard and ylang-ylang.

The present invention also provides pesticidal compounds. The insecticidal compound is preferably selected from compounds having insecticidal activity among the compounds forming the plant essential oil of the present invention.

More preferably, the compound has insecticidal activity against mites, and the insecticidal compound may be asarone of formula 1, cedrol of formula 2, acetyleugenol of formula 3, or iso of formula 4. Eugenol, β-thujaplicine of formula 5, (+)-panchon ((+)-fenchone) of formula 6, (-)-panchon ((-)-fenchone of formula 7 ), 3-methoxybenzaldehyde of formula 8, precocene (7-methoxy-2,2-dimethyl-chromene) of formula 9, beta-naphthyl acetate of formula 10 (acetic acid) β-naphthyl ester, anionic acid (4-methoxybenzoic acid) of formula 11, bornyl acetate of formula 12, safrole of formula 13, alpha-humulene of formula 14 humulene: α-caryophyllene, terpinolene of formula 15, allylanisole of formula 16, trans-beta-pi of formula 17 (Trans -β-pinene), an alpha of formula 18-pinene (α-pinene), beta of the formula (19) of the three-Karen (3-carene) and formula (20) - karioh propylene ((-) - (trans) -caryphyllene, β-caryophyllene) at least one selected from the group consisting of

The present invention also provides a pesticide composition. The insecticide composition preferably contains 0.01 to 40% by weight of the plant essential oil alone or in a mixture, and preferably includes 0.01 to 40% by weight of the insecticidal compound in the form of a single or mixture. However, the content of the plant essential oil or insecticidal compound is more preferably applied differently depending on the formulation of the pesticide, spraying method.

The composition is used where pests are inhabited, and the use method is preferably a coating treatment, dipping treatment, fumigation treatment, and the like. The formulation of the composition may be a liquid, emulsion, smoker, fumigant, coating agent, granules and the like.

In addition, the composition may further include an excipient according to the method of use. The excipient preferably uses an appropriate material according to the use and application method of the composition, more preferably a material included in a conventional pesticide formulation.

Hereinafter, examples of the present invention will be described. The following examples are only for illustrating the present invention and the present invention is not limited to the following examples.

Plant essential oil

Plant essential oils of Table 1 were purchased from Jinhyang Co., Ltd.

Refined name Raw material The name Country of origin extraction Thyme white Timus vulgaris Lamiaceae France Lobe Ked oil Juniusus oxycedrus Cypressaceae Portugal Lobe Cardamom Seilon Elettaria cardamomum Ginger France Fruit Juniper berry Juniperus Communis Cypressaceae France Fruit Caraway Seed Carum carvi Morphology France strain Tagetes Tattettes glandulifera Chrysanthemum Hungary Lobe Chamomile Roman Anthemis nobilis Chrysanthemum France Flower Coriander Coriandrum sativum Morphology Russia Fruit Citronella java Cymbopogon nardus Rice plant Indonesia Lobe sage Salvia officinalis Lamiaceae Spain Lobe Patchouli Pogostemon patchouli Lamiaceae Indonesia Lobe Bergamot Citrus bergamia Rhyme Italy skin Geranium Pelargonium graveolens Mouse grass France Flower Lime Discover 5F Citrus aurantifolia Rhyme United States of America skin Grapefruit Citrus paradisi Rhyme United States of America skin Claire sage Salvia sclerea Lamiaceae France Flower Bay Pimento racemosa Plating quantity France Lobe Peppermint Mentha piperita Lamiaceae United States of America Flower oregano Origanum vulgare Lamiaceae Morocco Lobe Myrtle Myrtus communis Plating quantity Morocco Lobe Cloverlip Eugenia caryophyllata Ash Tree Madagascar Lobe Eucalyptus Eucalyptus globulus Plating quantity China Lobe Rosewood Aniba rosaeodora Camphoraceae Brazil tree Tangerine Citrus reticulata Rhyme Italy Flower Tea tree Melaleuca tenifolia Plating quantity Australia Lobe Cedar Wood Cedus atlantica Cypressaceae United States of America tree

Lemon Eucalyptus Eucalyptus citriodora Plating quantity Australia Lobe Warmwood Artemesia absinthium Chrysanthemum United States of America Plant Frankensen Boswellia carterii Sumac Oman Suzy Rosemary Rosemarinus officinalis Lamiaceae Spain Flower Black Pepper Piper nigrum Peppercorns India Fruit Palmarosa Cymbopogon martini Rice plant Brazil Plant Pettigrain Citrus aurantium Rhyme United States of America Lobe Thyme red Timus vulgaris Lamiaceae France Lobe Mandarin Citrus reticulata Rhyme Brazil skin Funny Abies alba Pine tree Canada Conifer Marjoram Timus masticina Lamiaceae India Lobe Lemongrass Cymbopogon citratus Rice plant India Lobe Phenyl Royal Menta pulegium Lamiaceae Europe Lobe Vetiver Haiti Vetivera zizanoides Rice plant India Plant Lemon 10Fold Citrus limonum Rhyme Italy skin Spearmint Mentha spicata Lamiaceae United States of America Flower Clove Bird Eugenia caryophyllata Ash Tree Madagascar Flower buds Pimento Berry Capsicum annuum var. Angulosum Eggplant France Fruit Jinger Zinger officinalis Ginger Sri Lanka Root Nut Mug Myristica fragrans Nutmeg Indonesia Fruit lavender Ravendula hybrida Lamiaceae France Flower Bitter Orange Citrus aurantium Rhyme France skin Orange Citrus sinensis Rhyme Brazil skin Cypress Cupressus sempervirens Cypressaceae France skin Sandalwood Santalum album Unidirectional India tree basil Oxicum basilicum Lamiaceae India Flower

Example 1 Insecticidal Activity Assay for Stored Pests

(1) Breeding of stored pests

Plardia interpunctella (Hubner) larvae, larvae Cadra cautella (Walker), larvae of Sitophilus oryzae L. and Callosobruchus chinensis L.) adults were used. Hwaranggok Moth and Zalalak Moth are plastic containers (20 x 50) containing cornmeal, wheat flour, Ebioze, 3 vitamins and glycerin (200: 50: 3.5: 2.2: 60.8, w / w) 20 x 15 cm). The rice weevil was raised in a plastic container (20 x 20 x 15 cm) containing brown rice, and the red weevil was raised in a plastic container (26 x 30 x 20 cm) containing red beans. All the conditions of breeding were as follows: temperature 28 ± 1 ℃, relative humidity 55 ± 5%, and light conditions 16: 8 hours (day and night).

(2) insecticide test

50 [mu] l of each plant essential oil was treated with a micropipette in Whatman No. 2, 4.25 cm in diameter. Treated litchi was placed in Petri dishes (5 cm in diameter, 3.5 cm in height). Hwaranggok moth larvae and Zulalam moth larvae were fed with homologous 4-5 g of food, and rice weevil was also counted 15-20 tons of brown rice and put them in petri dishes. The red bean weevil was treated with 5 μl at the baseline, and no food was provided. After treatment was put in each treatment and control, the lid was closed and examined for pesticidal activity after 24 hours. Treatments were tested under the same conditions as for breeding. The presence or absence of pesticides was regarded as insecticide if there was no movement by stimulating each appendage with a fine brush or tapping the petri dish above or the bottom by hand. All the controls only put in place.

After three replicates, the mean was converted to insecticide.

(3) Insecticidal effect on jollalak moth

Insecticide activity of plant essential oil was treated with 50 μl per litter to investigate the insecticidal activity against the Jouleolam moth larvae and the insecticidal rate of 50 μl of the essential oil was shown in Table 2 below.

Refined name Insecticide,% 1 day 2 days Cardamom Seilon 80 90 Juniper berry 20 50 Tagetes 30 75 sage 90 95 Myrtle 100 - Eucalyptus 100 - Frankensen 20 65 Rosemary 100 - Funny 30 60 Marjoram 100 - Penny Royal 20 50 Vetiver Haiti 5 20 Nut Mug 85 100 Cypress 95 100 basil 70 70 Control 0 10

As shown in Table 2, myrtle, eucalyptus, rosemary and marjoram exhibited 100% insecticidal activity against the larval moth larvae, and nutmeg and cypress also showed 100% insecticidal rate after 48 hours of treatment.

(4) Insecticidal effect on Hwaranggok Moth

 Plant essential oils were treated with 50 μl per litter to investigate the insecticidal activity against the larval moth larvae, and the insecticides obtained by treating the essential oil with 10 μl level were shown in Table 3 below.

Refined name 50 μl / excitation Refined name 10 μl / excitation Insecticide,% Insecticide,% 1 day 2 days 1 day 2 days 3 days Cardamom Seilon 60 60 Juniper berry 15 15 20 Juniper berry 100 - sage 30 65 65 Tagetes 0 20 Myrtle 100 - - sage 90 90 Eucalyptus 100 - - Myrtle 100 - Tagetes 15 30 30 Eucalyptus 100 - Frankensen 30 35 35 Frankensen 100 Rosemary 85 90 93 Rosemary 100 - Funny 33 33 33 Funny 100 - Marjoram 100 - - Marjoram 100 - Penny Royal 3 57 67 Penny Royal 70 80 Vetiver Haiti 17 20 20 Vetiver Haiti 90 90 Nut Mug 70 97 100 Nut Mug 90 100 Cypress 50 50 50 Cypress 100 - Control 0 0 0 basil 30 40 Control 10 10

In Table 3, juniper berry, Frankensens, funnel, myrtle, eucalyptus, rosemary, marjoram and cypress showed 100% insecticide rate against H. pylori, and nutmeg also 100% after 48 hours of treatment. . In addition, the pesticide rate of Hwak Moth larvae was investigated by reducing the concentration to 10 μl for essential oils with more than 90% pesticide rate. As a result, myrtle, eucalyptus and marjoram reached 100% insecticidal rate after 24 hours of treatment, nutmeg also showed 100% insecticidal activity after 3 days of treatment, and rosemary showed strong insecticidal activity of 93%.

(5) Insecticidal rate for red bean weevil

The plant essential oil was treated with 5 μl per litter to investigate the insecticidal activity against adult red bean weevil, and the insecticidal rate is shown in Table 4.

Insecticide,% 24 hours after treatment Thyme white 100 Warmwood 100 Ked oil 100 Frankensen 100 Cardamom Seilon 100 Rosemary 100 Juniper berry 100 Black Pepper 100 Caraway Seed 100 Palmarosa 100 Tagetes 100 Pettigrain 100 Chamomile Roman 100 Thyme red 100 Coriander 100 Mandarin 100 Citronella java 100 Funny 100 sage 100 Marjoram 100 Patchouli 60 Lemongrass 100 Bergamot 100 Phenyl Royal 100 Geranium 100 Vetiver Haiti 100 Lime Discover 5F 100 Lemon 10Fold 100 Grapefruit 100 Spearmint 100 Claire sage 100 Clove Bird 100 Bay 100 Pimento Berry 100 Peppermint 100 Jinger 60 oregano 100 Nut Mug 100 Myrtle 100 lavender 100 Cloverlip 100 Bitter Orange 100 Eucalyptus 100 Orange 100 Rosewood 100 Cypress 100 Tangerine 100 Sandalwood 60 Tea tree 100 basil 10 Cedar Wood 70 Control 10 Lemon Eucalyptus 100

Time White, Ced Oil, Cardamom Celion, Juniper Berry, Caraway Seed, Tagetes, Chamomile Roman, Coriander, Citronella Java, Sage, Bergamot, Geranium, Lime Dis 5F, Grapefruit, Clary Sage, Bay, Peppermint, oregano, myrtle, cloverleaf, eucalyptus, rosewood, tangerine, tea tree, lemon eucalyptus, warmwood, francenseense, rosemary, black pepper, palmarosa, pettigrain, thyme red, mandarin, funnel, marjoram, Lemongrass, phenylroyal, vetiver hayty, lemon 10fold, spearmint, clove bud, pimento berry, nut mug, lavender, bitter orange, orange and cypress showed 100% pesticidal rate against red bean weevil.

(6) insecticidal rate for rice weevil

 The plant essential oils were treated with 50 μl and 10 μl per litter to investigate the insecticidal activity against adult rice weevil, and are presented in Table 5 below.

Refined name Insecticide,% Refined name Insecticide,% 50 μl 10 μl 50 μl 10 μl 1 day 1 day 2 days 1 day 1 day 2 days basil 100 3 30 Lemon 10Fold 100 7 47 Bay 100 20 53 Mandarin 97 3 17 Bergamot 100 0 7 Marjoram 100 17 27 Bitter Orange 100 0 27 Myrtle 100 0 30 Black pepper 100 10 30 Nut Mug 100 17 35 Ked oil 100 23 27 Orange 100 0 3 Caraway Seed 100 20 33 oregano 100 20 60 Cardamom Sailron 100 30 47 Palmarosa 100 0 20 Cedar Wood 23 0 27 Penny Royal 100 75 100 Chamomile Roman 100 0 3 Pengali 100 23 60 Cypress 100 0 3 Petite grain 100 3 17 Citronella java 100 35 30 Pimento Berry 100 3 25 Claire sage 100 10 40 Peppermint 0 7 70 Clovebird 100 3 3 Rosemary 100 0 25 Cloblip 100 3 30 Rosewood 100 3 45 Coriander 100 97 100 Sandalwood 100 0 3 Eucalyptus 100 27 20 sage 100 7 27 Funny 100 0 27 Spearmint 100 27 83 Frankensen 100 27 10 Tagetes 100 17 20 Geranium 100 0 20 Tangerine 100 3 17 Jinger 100 20 30 Tea tree 100 3 15 Grapefruit 67 0 17 Thyme white 100 3 20 Juniper berry 100 10 23 Thyme red 100 27 35 lavender 100 17 10 Vetiver Haiti 0 13 35 Lemon Eucalyptus 100 3 33 Warmwood 100 60 95 Lemongrass 100 17 20 Ylang-ylang 57 3 47 Lime Discover 5F 100 30 40 Control 0 0 7

50 μl of plant essential oils were treated to adult rice weevil. , Geranium, lime dis 5F, clary sage, bay, oregano, myrtle, cloblip, eucalyptus, rosewood, tangerine, tea tree, lemon eucalyptus, warmwood, francenseense, rosemary, black pepper, palmarosa, petite grain , Thyme red, mandarin, funnel, marjoram, lemongrass, penny royal, lemon 10fold, spearmint, clove bud, pimentoberry, ginger, nut mug, sandalwood, bitter orange, orange, cypress, sandalwood and basil After 24 hours of treatment, 100% insecticide was shown. In 10 μl of plant essential oils, coriander (100%), penny royal (100%) and wormwood (95%) showed high insecticides.

Example 2: Insecticidal Activity Assay against Mites

(1) mite breeding

American house dust mite, Dermatophagoides farinae , European house dust mite, Dermatophagoide pteronyssinus , and Tyrophagus putrescentiae maintain a relative humidity of 75 ± 5% and a temperature of 25 ± 1 ° C. The animals were bred in a nursery and were fed a 12.5 x 10.5 x 5 cm container with a 1: 1 mixture of ^pomace and multivitamin ( ^Ebioze® ). In order to maintain humidity, saline was poured into the bottom of a container having a volume of 17.5 x 17.5 x 17.5 cm in the breeding room. The house dust mite species are purely susceptible strains that have not been exposed to acaricides or pesticides.

(2) Insecticide Test Method (Fiber Diffusion Method)

1 mg or 0.5 mg of plant essential oils were dissolved in 40 μl ethanol and treated in a 4.2 mm diameter black filter to evaporate the solvent for 5 minutes. Laying a room in a Petri dish (diameter 5.5 cm, height 0.9 cm, volume of 21.4 cm ^3), and closed the lid to radiation ssikeul each house dust mite adults 25 objects. All assays were identical to farming conditions and no food was provided. Assay results were examined under an optical microscope (20 times) after 24 hours of treatment, the subject was considered to have died without any movement by stimulating the appendage and torso with a fine brush.

(3) insecticidal rate

1 mg or 0.5 mg of plant essential oils were treated with large dust mites and vertical dust mites, and the insecticidal rate after 24 hours was measured and shown in Table 6.

Refined name 1 mg 0.5 mg Refined name 1 mg 0.5 mg Vertical Pattern Dust Mite Large leg mites Large leg mites Vertical Pattern Dust Mite Large leg mites Large leg mites Thyme white 100 100 40 Warmwood 64 - - Ked oil 100 96 6 Frankensen 28 - - Cardamom Seilon 47 - - Rosemary 0 - - Juniper berry 6 - - Black Pepper 0 - - Caraway Seed 100 64 2 Palmarosa 100 100 2 Tagetes 0 - - Petite grain 16 - - Chamomile Roman 0 - - Thyme red 72 - - Coriander 80 - - Mandarin 0 - - Citronella java 100 100 8 Funny 92 - - sage 0 - - Marjoram 2 - - Pengali 98 32 24 Lemongrass 100 100 8 Bergamot 0 - - Penny Royal 100 0 0 Geranium 98 100 12 Vetiver Haiti 88 - - Lime Discover 5F 98 2 8 Lemon 10Fold 100 76 2 Grapefruit 0 - - Spearmint 0 - 0 Claire sage 4 - - Clove Bird 100 100 100 Bay 100 - - Pimento Berry 100 94 98 Peppermint 100 98 36 Jinger 48 - - oregano 100 100 48 Nut Mug 100 4 4 Myrtle 0 - - Sandalwood 64 - - Cloblip 100 100 46 Bitter Orange 82 4 4 Eucalyptus 0 - - Orange 0 - - Rosewood 2 - - Cypress 0 - - Tangerine 0 - - Sandalwood 96 88 - Tea tree 0 - - basil 20 - - Cedar Wood 82 - - Control 0 0 - Lemon Eucalyptus 82 - -

Time White, Ced Oil, Caraway Seed, Citronella Java, Peppelli, Geranium, Lime Dis 5F, Bay, Peppermint, Oregano, Clovelip, Palmarosa, Lemongrass, Penny Royal, Lemon 10Fold, Clove Bird, Pimento Berry , Nut mugs and sandalwood showed a high rate of 100% of vertical ticks. In addition, thyme white, ked oil, citronella java, geranium, peppermint, oregano, clovelip, palmarosa, lemongrass and clove bud showed 100% insecticidal rate against large dust mites. Also, at 0.5 mg treatment level, clover buds showed 100% insecticidal rate against large leg dust mites.

Example 3: Insecticidal Activity Assay against Hygienic Pests

(1) mosquito

Culex pipiens pallens larvae were bred for calf feed (Samyang Co., Ltd.) and fed into a container containing water (20 x 20 x 20 cm). Breeding conditions were relative humidity 60 ± 5%, temperature 27 ± 1 ℃, light conditions 13:11 (day and night).

(2) insecticide test

To test the mosquito larvae, put 230 ml of tap water in a paper cup (250 ml), and add only 3 vital mosquito larvae (1 week after hatching) with a dropper (10 ml), and put 25 objects into another diet cup. gave. Proper amounts of plant essential oils were dissolved in 1 ml of acetone or ethanol at 100 ppm concentration, and 1 ml of 100 ppm triton X-100 and 230 ml of tap water were mixed in a paper cup. To this mosquito larvae were spun and finally tap water was added to 250 ml. The results were examined after 24 hours, and no feed fry were fed.

(3) insecticide rate for mosquitoes

Insecticidal rate according to the concentration of the larvae larvae of plant essential oils are shown in Table 7.

Refined name Insecticidal rate (mean ± standard deviation,%) 50 ppm 25 ppm 12.5 ppm Thyme white 100 ± 0.0 16 ± 5.7 0 ± 0.0 Grapefruit 100 ± 0.0 99 ± 2.3 29 ± 6.1 Tangerine 100 ± 0.0 83 ± 11.5 8 ± 8.0 Tea tree 100 ± 0.0 12 ± 6.9 0 ± 0.0 Thyme red 100 ± 0.0 15 ± 2.3 0 ± 0.0 Mandarin 100 ± 0.0 28 ± 4.0 1.3 ± 2.3 Penny Royal 100 ± 0.0 0 ± 0.0 0 ± 0.0 Nut Mug 100 ± 0.0 28 ± 12.0 0 ± 0.0 Bitter Orange 100 ± 0.0 21.3 ± 2.3 0 ± 0.0 Orange 100 ± 0.0 38.7 ± 11.5 1.3 ± 2.3 Cypress 99 ± 2.3 2.7 ± 2.3 1.3 ± 2.3 Sandalwood 99 ± 2.3 6.7 ± 4.6 1.3 ± 2.3 basil 96 ± 4.0 5.3 ± 6.1 2.7 ± 2.3 Myrtle 93 ± 2.3 7 ± 2.3 0 ± 0.0 Funny 96 ± 4.0 1 ± 2.3 0 ± 0.0 Marjoram 95 ± 2.3 1 ± 2.3 1.3 ± 2.3 Chamomile Roman 80 ± 4.0 0 ± 0.0 0 ± 0.0 Pengali 81 ± 2.3 0 ± 0.0 0 ± 0.0 Cloblip 85 ± 2.3 0 ± 0.0 0 ± 0.0 Lemongrass 83 ± 2.3 - - Jinger 83 ± 2.3 - - Sandalwood 76 ± 4.0 - - Control 0 ± 0 0 ± 0.0 0 ± 0.0

Thyme White, Grapefruit, Tangerine, Tea Tree, Thyme Red, Mandarin, Penny Royal, Nut Mug, Bitter Orange and Orange showed 100% insecticidal rate at 50 ppm levels against mosquitoes. Cypress, sandalwood, basil, myrtle, funnels and marjoram have exhibited a pesticidal rate of over 90% against mosquitoes. Chamomile Roman, Peppeli, Cloveblip, Lemongrass, Zinger Essential Oil and Lavender showed more than 76% pesticide rate. At 25 ppm of plant essential oils, grapefruit showed an excellent pesticidal rate of 99% and tangerine essential oils of 83%.

Example 4 Insecticidal Activity Assay against Hygienic Pests

(1) breeding of wheels

The wheels ( Blattella germanica L.) were bred in a breeding room with a relative humidity of 60 ± 5%, a temperature of 27 ± 1 ℃, and light conditions 13:11 (day and night), and a cylindrical container (diameter 30 × height 30 cm). The calf feed (Samyang) was put in.

(2) insecticide test

Insecticidal activity against the wheel was determined by the filter paper diffusion method. A certain amount of plant essential oil was dissolved in 100 μl methanol, treated in a filter paper (4.25 cm in diameter), and then volatilized for 5 minutes. The treated space was placed in a translucent plastic cup (5.5 cm in diameter x 7 cm in height), and 10 adults (5 male and female each) were spun and the results were examined 24 hours after the treatment.

(3) insecticide rate on wheels

Insecticidal rate for the wheel of plant essential oil is shown in Table 8.

Refined name Insecticide,% Refined name Insecticide,% 40 μl 10 μl 40 μl 10 μl Thyme white 100 5 Nut Mug 100 75 Ked oil 100 15 Orange 100 5 Caraway Seed 100 75 Cypress 100 - Coriander 100 15 basil 100 60 sage 100 55 Rosewood 90 - Grapefruit 100 0 Lemon Eucalyptus 90 - Peppermint 100 10 Rosemary 90 5 Myrtle 100 5 Mandarin 90 5 Eucalyptus 100 10 Cardamom Seilon 90 0 Tangerine 100 5 Clove Bird 80 - Tea tree 100 20 Pimento Berry 80 - Warmwood 100 - Jinger 80 - Black Pepper 100 - lavender 80 - Thyme red 100 5 Bitter Orange 80 - Funny 100 5 Lime Discover 5F 70 - Marjoram 100 5 Bay 70 - Lemongrass 100 - Frankensen 70 - Penny Royal 100 90 Control 0 0 Spearmint 100 70

When 40 μl of plant essential oil is treated, thyme white, ked oil, caraway seed, coriander, sage, grapefruit, peppermint, myrtle, eucalyptus, tangerine, tea tree, wormwood, black pepper, thyme red, funnel, Marjoram, lemongrass, penny royal, spearmint, nut mugs, orange, cypress and basil showed 100% pesticidal rate for the wheels. Rosewood, Lemon Eucalyptus, Rosemary, Mandarin, Cardamom Selon, Clove Bud, Pimentoberry, Zinger, Lavender, Bitter Orange, Lime Dis 5F, Bay and Francensense have high pesticide rates.

When 10 µl of plant essential oils were treated, penny royal, spearmint and nut mug showed high insecticidal rate.

Example 5: Insecticidal Activity Assay against Ants

(1) Insecticide Test

Loved ants were collected from ordinary households in Suwon. Insecticidal activity assay was carried out by the room diffusion method of Example 2.

(2) insecticide rate for ants

Insecticidal rate of the essential oil of plant essential oil is shown in Table 9.

Refined name Insecticide,% 30 minutes 1 hours 90 minutes 2 hours 150 minutes 3 hours 210 minutes 4 hours 270 minutes 5 hours Thyme white 10T 10T 100 100 100 100 100 100 100 100 Ked oil 0 10T 2d, 8T 2d, 8T 2d, 8T 2d, 8T 2d, 8T 2d, 8T 100 100 Cardamom Seilon 10T 10T 10T 10T 10T 2d, 8T 2d, 8T 2d, 8T 100 100 Juniper berry 10T 10T 1d, 9T 1d, 9T 1d, 9T 2d, 8T 2d, 8T 2d, 8T 3d, 7T 100 Caraway Seed 0 5d, 5T 7d, 3T 9d, 1T 9d, 1T 100 100 100 100 100 Chamomile Roman 5T 10T 10T 10T 10T 10T 10T 10T 10T 100 Coriander 10T 10T 100 100 100 100 100 100 100 100 Citronella java 10T 10T 100 100 100 100 100 100 100 100 sage 4d, 6T 100 100 100 100 100 100 100 100 100 Bergamot 0 10T 10T 10T 10T 4d, 6T 4d, 6T 4d, 6T 4d, 6T 100 Lime Discover 5F 0 0 0 0 0 0 0 0 10T 100 Claire sage 3T 4d, 6T 4d, 6T 4d, 6T 4d, 6T 5d, 5T 5d, 5T 5d, 5T 6d, 4T 100 Bay 0 0 100 100 100 100 100 100 100 100 Peppermint 10T 1d, 9T 100 100 100 100 100 100 100 100 oregano 10T 8T 5d, 5T 5d, 5T 5d, 5T 100 100 100 100 100 Myrtle 10T 1d, 9T 4d, 6T 4d, 6T 4d, 6T 4d, 6T 4d, 6T 4d, 6T 4d, 6T 100 Cinnamon oil 0 10T 100 100 100 100 100 100 100 100 Eucalyptus 10T 3d, 7T 3d, 7T 100 100 100 100 100 100 100 Control 0 0 0 0 0 0 0 0 0 0

In Table 9, the letter 'd' means dead ant population and 'T' means population causing severe trembling.

Within 1 hour of plant essential oil treatment, sage showed 100% insecticide rate against the antler, and within 1 hour 30 minutes, thyme white, coriander, citronella java, bay, peppermint and cinnamon oil were 100% insecticide rate to the antler. Showed. Other plant essential oils showed 100% pesticide rate within 5 hours. The most rapid of these are thyme white, cardamom selon, coriander, sage and eucalyptus.

Example 6: Insecticidal Activity Assay for Agricultural Pests

(1) Insecticide Test

Tomato leaves and Trialeurodes vaporariorum adults grown in 3 to 4 weeks are placed in a 135 cm 3 transparent cylindrical plastic barrel, and the leaves are wrapped with a cotton ball soaked in water and wrapped with foil to prevent loss of moisture. It was. Each plant essential oil was dissolved in 100 μl of 95% ethanol by concentration, treated with 2 × 2 cm (horizontal × vertical) space, dried at room temperature for 1 minute, and then placed in a plastic container. After 24 hours, the insecticide rate of greenhouse powder was investigated.

(2) Insecticidal rate for greenhouse powder

Table 10 shows the insecticidal rate for greenhouse powder of plant essential oils.

Refined name Insecticide,% Refined name Insecticide,% Concentration per litter, μl Concentration per litter, μl 5 2.5 One 0.5 0.25 5 2.5 One 0.5 0.25 Thyme white 100 100 100 100 100 Frankensen 100 100 100 - - Ked oil 100 100 100 30 - Rosemary 100 100 70 - - Cardamom Seilon 100 100 100 50 - Black Pepper 100 100 90 - - Juniper berry 100 100 40 25 - Palmarosa 100 100 100 - - Caraway Seed 100 100 100 100 - Petite grain 100 100 100 - - Tagetes 100 100 10 10 - Thyme red 100 100 100 - - Chamomile Roman 100 100 30 30 - Mandarin 100 100 20 - - Coriander 100 100 100 100 - Funny 100 100 100 - - Citronella java 100 100 100 90 - Marjoram 100 100 20 - - sage 100 100 100 30 - Lemongrass 100 100 100 - - Pengali 100 100 100 100 100 Penny Royal 100 100 100 - - Bergamot 100 100 100 90 50 Vetiver Haiti 100 100 90 - - Geranium 100 100 100 100 100 Lemon 10Fold 100 100 100 - - Lime Discover 5F 100 100 100 100 100 Spearmint 100 100 100 - - Grapefruit 100 100 100 10 5 Clove Bird 100 100 100 - - Claire sage 100 100 100 100 100 Pimento Berry 100 100 100 - - Bay 100 100 100 100 75 Jinger 100 100 100 - - Peppermint 100 100 100 100 100 Nut Mug 100 100 100 - - oregano 100 100 100 100 100 lavender 100 100 100 - - Myrtle 100 100 100 30 10 Bitter Orange 100 100 100 - - Cloblip 100 100 100 - - Orange 100 100 100 - - Eucalyptus 100 100 100 100 100 Cypress 100 100 100 - - Ylang-ylang 90 50 10 - - Sandalwood 100 100 100 - - Rosewood 100 100 100 - - basil 100 100 35 - - Tangerine 100 100 100 - - Cinnamon oil 100 100 100 100 100 Tea tree 100 100 100 - - Horseradish Oil 100 100 100 100 100 Lemon Eucalyptus 100 100 100 - - Mustard oil 100 100 100 100 100 Warmwood 100 100 100 - - Control 10 5 5 5 5

At 5 μl of plant essential oils, Time White, Caraway Seed, Ked Oil, Cardamom Selon, Juniper Berry, Tagetes, Chamomile Roman, Coriander, Citronella Java, Sage, Peppeli, Bergamot, Geranium, Lime Dis 5F , Grapefruit, clary sage, bay, peppermint, oregano, myrtle, clovelip, eucalyptus, ylang-ylang, rosemary, tangerine, tea tree, lemon eucalyptus, warmwood, francense, black pepper, palmarosa, petite grain, Thyme red, mandarin, funnel, marjoram, lemongrass, penny royal, vetiver hayty, lemon 10fold, spearmint, clove bud, pimento berry, nut mug, ginger, lavender, bitter orange, orange, cypress, sandalwood, Basil, horseradish oil, cinnamon oil and mustard oil showed 100% insecticidal rate against greenhouse powder.

Even at a low treatment level of 0.25 μl of plant essential oils, Pepelli, Geranium, Lime Dis 5F, Claricle Sage, Peppermint, Oregano, Myrtle, Eucalyptus, Horseradish Oil, Cinnamon Oil and Mustard Oil are 100% of the greenhouse powder. Pesticide rate was shown.

Example 7: Insecticidal Activity Assay of Plant-Derived Compounds

Insecticidal activity against compounds known as major compounds of plant essential oils was assayed. Each compound was purchased from Aldrich.

1 mg, 0.5 mg, 0.25 mg of the compound was treated with cloth to observe the rate of application for the large-legged dust mites, the vertical patterned mites, and the long-haired dust mites.

Compound (mg / fiber) Agitation rate,% Large leg mites Vertical Pattern Dust Mite Long hair mite One 0.5 0.25 One 0.5 0.25 One 0.5 0.25 Asaron 100 99 92 100 100 91 77 8 0 Cedrol 100 40 4 92 33 16 100 64 0 Alpha-terpineol 100 100 81 100 97 92 15 12 0 Carvacrol 100 100 93 100 91 27 100 100 0 Acetyleugenol 100 68 4 100 33 8 100 39 0 Eugenol 100 100 35 100 32 19 100 100 37 Isoeugenol 100 100 88 100 48 47 100 100 88 Beta-tujaplicine 100 - - 100 - - - - - (+)-Fanchon ((+)-fenchone) 100 - - - - - - - - (-)-Fanchon ((-)-fenchone) 100 - - - - - - - - Beta-caryophyllene ((-)-( trans ) -caryphyllene, β-caryophyllene) 80 - - - - - - - - Alpha-humulene (α-caryophyllene) 98 - - - - - - - - (-)-Methol ((-)- p -menthan-3-ol, (-)-menthol) 100 - - - - - - - - Trans-beta-pyrene (trans -β-pinene) 94 - - - - - - - - 3-carene 91 - - - - - - - - 3-methoxybenzaldehyde 100 - - - - - - - - Precocene (7-methoxy-2,2-dimethyl-chromene) 100 - - - - - - - - Acetic acid β-naphthyl ester 100 - - - - - - - - Allylisole 90 - - - - - - - - Anicin acid (4-methoxybenzoic acid) 100 - - - - - - - - Bornyl acetate 100 - - - - - - - - Safrole 100 - - - - - - - - Terpinolene 90 - - - - - - - - Trans-anethole (trans -anethole) 100 - - - - - - - - Alpha-pinene 66 - - - - - - - - Benzyl benzoate 100 100 87 100 100 100 98 87 45 DEET 99 68 31 87 64 28 95 83 69 Dibutyl phthalate 100 100 94 100 100 96 - - -

When 1 mg of plant-derived compound was treated with asarone, alpha-terpineol, cedrol, carbacrol, acetyleugenol, eugenol, isoeugenol, beta-tuzaflisin, (+)-panchon, (- 100% insecticidal rate of) -Panchon, (-)-Menthol, 3-methoxybenzaldehyde, Fricosene, beta naphthyl ester, anicin acid, carbonyl acetate, saprolol and trans-anetol Showed. In addition, when treated with 0.5 mg, asaron (99%), cedrol (100%), carbacroll (100%), eugenol (100%), isoeugenol (100%) showed a high pesticide rate, At the 0.25 mg level, the insecticidal rate was over 90%. The benzyl benzoate and dibutyl phthalate used in the control group exhibited pesticidal rates of 100% at 0.5 mg and higher and 87% and 94% at 0.25 mg. In addition, Dit showed 99% pesticide rate only at 1 mg treatment level, indicating that the active compounds showed better insecticidal rate.

100% of asarone, cedrol, carbacroll, acetyleugenol, eugenol, isoeugenol and beta-tuzaplicin were excluded from alpha-terpineol (92%) at 1 mg treatment for longitudinal dust mites. It showed high acaricide activity. At the 0.5 mg and 0.25 mg levels, asaron, cedrol and carbacrol showed high insecticidal rates. The benzyl benzoate and dibutyl phthalate used in the comparison group showed a strong acaricide activity of more than 96% at 0.25 mg or more level, as in the large-legged dust mite, while Dit showed 87% of acaricide at the 1 mg level. Compared to the active compounds showed similar or lower activity.

At 1 mg treatment for long hair mites, alpha-terpineol, carbachol, acetyleugenol, eugenol and isoeugenol each exhibited a 100% pesticide rate. Carbacrol, eugenol and isoeugenol showed 100% insecticide at 0.5 mg level, and isoeugenol showed a high insecticide rate of 88% even at 0.25 mg. The benzylbenzoate and diet used as comparative groups showed 98% and 95% activity at 1 mg level and 87% and 83% at 0.5 mg level, respectively, but showed low acaricide activity at 0.25 mg level. Therefore, the most well observed isoeugenol in the present invention was observed to have a better acaricide activity.

Since the plants, plant essential oils and compounds of the present invention have excellent insecticidal action against pests, they can be used as natural insecticides to overcome the problems caused by synthetic insecticides.

Claims (18)

Pogostemon patchouli , Eucalyptus citriodora , Boswellia carterii , Abies alba , Mentha pulegium , Bettyvera zijanoides ( Vetivera zizanoides) , Mentha spicata , Capsicum annuum var. Angulosum , Zinger officinalis , Cupressus sempervirens , Elle Talia Serdamomum , Carum carvi , Tagettes glandulifera , Anthemis nobilis , Coriandrum sativum , Symbopogon Narders ( Cymbopogon nardus ), Mentha piperita , Myrtus communis , Eugenia caryophyllata , Aniba rosaeodora , Three Insecticidal composition comprising 0.01 to 40% by weight of essential oils extracted from at least one plant selected from the group consisting of Cedus atlantica , Thymus masticina , Santalum album and mustard . delete The method of claim 1, wherein the Eletaria Cerdamomum, Karum Carvi, Tagetes Glandolipera, Antemis Nobilis, Coriandrum Sativaboom, Cymbogongon Narders, Pogostemon Patchuli, Menta Piperita, Mig Tus Comunis, Eugenia Karyopilata, Aniva Rososadora, Cedus Atlantica, Eucalyptus Citrudora, Boswellia Carteri, Abies Alba, Timus Mastinasina, Menta Fullegium, Betivera Zijanoides, Essential oils extracted from at least one plant selected from the group consisting of Menta Spicata, Capsicum anum bur Agulosum, Zinger Officinalis, Cupresus Sempervirens and Santalum Alboom have been found to be effective against insect pests. Insecticidal composition. According to claim 1, wherein the essential oil extracted from at least one plant selected from the group consisting of Migtus Komunis, Timus mastisina and Cupresus Sempervirens has insecticidal properties against joolak moths Insecticidal composition. The essential oil of claim 1, wherein the essential oil extracted from at least one plant selected from the group consisting of Migtus Communis, Boswellia Carteri, Abies Alba, Timus mastisina, and Cupresus Sempervirens An insecticidal composition having insecticidal properties against. The method of claim 1, wherein the Eletaria Cerdamomum, Karum Carvi, Tagetes Glandolipera, Antemis Nobilis, Coriandrum Sativaboom, Cymbogongon Narders, Pogostemon Patchuli, Menta Piperita, Mig Tus Comunis, Aniva Rosaodora, Cedus Atlantica, Eucalyptus Citrudora, Boswellia Carteri, Abies Alba, Timus Masthina, Menta Fulleium, Bettyvera Zyzanoides, Menta Spicata, Eugenia Cary Essential oils extracted from at least one plant selected from the group consisting of yopilata, capsicum anum bur agulosum, jinger officinalis, and cupresus sempervirens are insecticidal for red bean weevil Composition. The method of claim 1, wherein the Eletaria Cerdarmomum, Karum Carvi, Tagetes Glandolipera, Antemis Nobilis, Coriandrum Sativaboom, Cymbogongon Narders, Pogostemon Patchuli, Migtus Komunis, Aniba Rososadora, Eucalyptus Citrudora, Boswellia Carteri, Abies Alba, Timus Mastina, Cympogon Citratus, Menta Furegium, Menta Spicata, Eugenia Caryopilata, Capsicum Anumber The essential oil extracted from at least one plant selected from the group consisting of Agulo island, Zinger Offisinalis, Cupresus Sempervirens and Santalum Alboom has insecticidal properties against rice weevil. The method of claim 1, wherein the Eletaria Serdamomum, Karum Carvi, Antemis Nobilis, Coriandrum Sativaboom, Simbopogon Narders, Pogostemon Patchouli, Menta Piperita, Migters Komunis, Eugenia Kar Yopilata, Aniva Rososadora, Eucalyptus Citridora, Boswellia Carteri, Abies Alba, Menta Fullegium, Menta Spicata, Capsicum Anumber Agulosum, Zinger Officinalis, Cupresum Sem The essential oil extracted from at least one plant selected from the group consisting of Fervirens and Santalum Alboom have insecticidal properties against sanitary pests. The essential oil of claim 1, wherein the essential oil extracted from at least one plant selected from the group consisting of the cymbopogon nartus, pogostemon patchuli, menta piperita, menta fulleum, capsicum annum bur agulosum and Santalum alboom An insecticidal composition having insecticidal properties against ticks. The method of claim 1, wherein the anthemis nobilis, pogostemon patchouli, migters comunis, eugenia karyopilata, abies alba, timus mastisina, menta pulleium, jinger officinalis, cupre The essential oil extracted from at least one plant selected from the group consisting of Sus Sempervirens and Santalum Alboom is insecticidal composition for mosquitoes. The method of claim 1, wherein the Eletaria cerdamomum, Karum Carvi, Coriandrum Sativam, Menta Piperita, Migtus Komunis, Aniva Rososadora, Eucalyptus Citridora, Boswellia Carteri, Avi At least one selected from the group consisting of S Alba, Timus Masthina, Menta Fulgeum, Menta Spicata, Eugenia Caryopilata, Capsicum Annum Bur Agulosum, Zinger Officinalis and Cupresus Sempervirens Essential oil extracted from the plant is insecticidal composition having mosquitoes against mosquitoes The plant of claim 1, wherein the plant is at least one selected from the group consisting of Eletaria cerdamomum, Karum Carvi, Antemis Nobilis, Coriandrum Sativaboom, Cympogongon Narders, Menta Piperita and Migtus Komunis. The essential oil extracted from the insecticidal composition having insecticidal properties against ants The method of claim 1, wherein the Eletaria Cerdamomum, Karum Carvi, Tagetes Glandolipera, Antemis Nobilis, Coriandrum Sativaboom, Cymbogongon Narders, Pogostemon Patchuli, Menta Piperita, Mig Tus Comunis, Eugenia Karyopilata, Aniva Rososadora, Eucalyptus Citrudora, Boswellia Carteri, Abies Alba, Timus Mastina, Menta Fulgium, Bettyvera Zyzanoides, Menta Spicata, Essential oils extracted from one or more plants selected from the group consisting of Eugeneia Caryopilata, Capsicum anumbur Agulosum, Zinger Ophisinalis, Cupresus Sempervirens, Santalum Alboom and Mustard may be used against agricultural pests. Insecticidal composition having insecticidal properties. Asarone, cedrol, acetyleugenol, isoeugenol, beta-tujaplicine, (+)-fanchon ((+)-fenchone), (-)-Panchon ((-)-fenchone), 3-methoxybenzaldehyde, precocene (7-methoxy-2,2-dimethyl-chromene), beta-naphthyl ester (acetic acid) at least one selected from the group consisting of acetic acid β-naphthyl ester, anionic acid (4-methoxybenzoic acid), safrole, allylisole, and 3-carene Insecticidal composition comprising 0.01 to 40% by weight. Tick insecticidal composition comprising 0.01 to 40% by weight of cymbopogon martini essential oil. An ant insecticidal composition comprising from 0.01 to 40% by weight of an essential oil of a plant selected from the group consisting of Pimento racemosa , cinnamon and mixtures thereof. An agricultural pest insecticidal composition comprising 0.01% to 40% by weight of essential oil of at least one plant selected from the group consisting of pimento racemosa, cymbopogon martini, cinnamon, horseradish and ylang ylang. 18. The composition of claim 17, wherein the agricultural pest is greenhouse dust.