KR102317362B1 - Composition and Method for Controling Pests Using an Extract of Myristica fragrans - Google Patents

Abstract

The present invention discloses a composition for controlling pests using a nutmeg extract having a controlling effect on pests and a method for controlling the same.

Description

Composition and Method for Controling Pests Using an Extract of Myristica fragrans

The present invention relates to a composition for controlling pests using an extract of nutmeg (Myristica fragrans) and a method for controlling the same.

Since pests not only directly reduce crop yields but also adversely affect quality, ultimately leading to economic loss of farms, the demand for pesticides for pest control for the purpose of increasing crop productivity has been continuously increasing. Pesticides that have been widely used so far are organic synthetic insecticides including DDT. These organic synthetic insecticides have caused a problem of biological toxicity by the food chain of the ecosystem because of their high toxicity and low degradability for all pests rather than effective insecticidal power against specific pests. . In addition, an increase in resistant pests due to the misuse of organic synthetic pesticides, a decrease in useful insects, an increase in agricultural pesticide residues, and pollution of water and soil are acting as risk factors for public health. In order to solve the problems of side effects, ecological destruction, and environmental pollution of these organic synthetic pesticides, it is an eco-friendly insecticide that has a high insecticidal effect and has a low persistence, is low toxicity to livestock, is not harmful to natural enemies, is environmentally safe, and targets only desired pests. Efforts are being made to develop

Among them, eco-friendly pesticides using plant extracts directly use plant extracts containing the plant's own defense substances against insects, nematodes, and plant pathogens, and have the advantage of being environmentally safe, low toxicity, and good decomposition.

Tussilagofarfara extract (Sungho Park, Chungnam National University, master's thesis, 2013), Quisqualis indica extract (Jin-seon Song, Kyungpook National University, master's thesis, 2013), Zanthoxylum Pipertum (Jeongyun Koh, Chonnam National University, master's thesis) Thesis thesis, 2017) extract, Dalmatian chrysanthemum ( Tanacetum cinerariifolium ) extract (Korean Patent No. 10-1885083), ivy extract (Korean Patent No. 10-1614095), oleifera seed extract (Korean Patent Publication No. 10-2019) -0032923) have been proposed as eco-friendly insecticides using plant extracts.

The present invention discloses a control activity against pests such as nutmeg (Myristica fragrans) extracts and the like.

An object of the present invention is to provide a composition for controlling pests using a nutmeg extract.

Another object of the present invention is to provide a method for controlling pests using a nutmeg extract.

Other objects or specific objects of the present invention will be set forth below.

The present invention, as confirmed in the following examples and experimental examples, nutmeg extract ( Nillaparvata lugens ), peach aphid ( Myzus persicae ), cucumber thrips ( Thrips palmi ), large-legged house dust mite ( Dermatophagoides farinae ) It was completed by confirming the insecticidal effect against

Therefore, the composition for controlling pests of the present invention is characterized in that it contains nutmeg extract as an active ingredient.

As used herein, the term "nutmeg extract" refers to the extraction of nutmeg leaves, stems, above-ground parts, rhizomes, roots, underground parts, or mixtures thereof with water, lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.), methylene chloride, Ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof It refers to an extract obtained by leaching using a supercritical extraction solvent such as carbon dioxide, pentane, or a fraction obtained by fractionating the extract. Any method such as reflux, heating, ultrasonic radiation, or supercritical extraction may be applied. In the case of a fractionated extract, it is meant to include a fraction obtained by suspending the extract in a specific solvent and mixing and standing still with a solvent having a different polarity, and a fraction obtained by column chromatography. In addition, the meaning of the extract includes a concentrated liquid extract or solid extract from which the extraction solvent is removed by freeze drying, vacuum drying, hot air drying, spray drying, or the like. Preferably, it means an extract obtained using water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof as an extraction solvent, particularly an extract obtained using methanol.

In addition, in the present specification, the term "active ingredient" means a component that can exhibit the desired activity alone or can exhibit activity together with a carrier that does not have the activity itself.

Also in the present specification, "pest control" means avoidance of pests or insecticide of pests. Here, the pests include Hemiptera pests, Thysanoptera pests, and Acarina pests. Specifically Hemiptera pests include aemyeolgu (Laodelphax striatellus), Brown Planthopper (Nilaparvata lugens), etc. myeolgugwa (Delphacidae) pests, kkeutdong maemichung (Nephotettix cincticeps), two points kkeutdong maemichung (Nephotettix virescens) maemichung and (Deltocephalidae) pests, cotton aphid, such as ( Aphis gossypii ), peach aphid ( Myzus persicae ), cabbage aphid ( Brevicoryne brassicae ), potato beard aphid ( Macrosiphum euphorbiae ), Aulacorthum solani aphid, millet border aphid ( Toxoptera citricidus padi xoptalosiphum ) ), such as aphids and (Aphididae) pests, pulsaek norinjae (Nezara antennata), the top bridge ant waist norinjae (Riptortus clavetus), leg waist norinjae (Leptocorisa chinensis), visible point round norinjae (Eysarcoris parvus), sseokdeong trees norinjae (Halyomorpha mista ) such as pentatomidae (Pentatomidae) pests, greenhouse whitefly (Trialeurodes vaporariorum), eunip whitefly (Bemisia argentifolii), such as a powder of Science (Aleyrodidae) pests, San Jose pod worm (Comstockaspis perniciosa), arrow pod worm (Unaspis citri), ruby pod worm ( Ceroplastes rubens ), such as Coccidea pests, and thrips pests include thrips ( Frankliniella occidentalis ), cucumber thrips ( Thrips palmi ), thrips ( Scirtothrips dorsalis ), thrips ( Scirtothrips dorsalis ) Thrips tabaci ), Taiwan thrips ( Frankliniella intonsa ), such as thrips family (Thr ipidae) pests, and Acarina pests include Tetranychidae pests, such as Tetranychus urticae , Panonychus citri , Eriophyidae pests, such as Aculops pelekassi primary dust mite (Polyphagotarsonemus latus) include house dust jindeugigwa (Pyroglyphidae) such as dust, insects eungaegwa (Tarsonemidae) pest, a large bridge house dust mite (Dermatophagoides farinae), house dust mite vertical pattern (Dermatophagoides pteronyssinus) and the like. Preferably, the insecticidal control activity is confirmed in the examples below ( Nillaparvata lugens ), peach aphid ( Myzus persicae ), cucumber thrips ( Thrips palmi ), large-legged house dust mite ( Dermatophagoides farinae ) means.

In the composition of the present invention, the active ingredient may be included in any amount (effective amount) according to the specific control use, formulation, etc. as long as it can exhibit pest control activity, etc., but a typical effective amount is 0.001 based on the total weight of the composition weight % to 20.0 weight %. Here, "effective amount" refers to the amount of the active ingredient included in the composition of the present invention, which can exhibit a pest control effect. Such effective amounts can be determined empirically within the ordinary ability of one of ordinary skill in the art.

In addition to the active ingredient, the composition of the present invention may include any compound or natural product-derived material known to have pest control activity in the art to enhance the increase in pest control activity. Such compounds or substances of natural origin are tetrazolinone compounds (

Korean Patent Publication No. 10-2015-0008863), halogen-containing organosulfur compounds (Korean Patent Publication No. 10-2010-0031518), alpha-alkoxyphenylacetic acid derivatives (Korean Patent Publication No. 10-2011-0057160), A pyrimidine compound (Korean Patent Publication No. 10-2006-0009324), a nitrile compound (Korean Patent Publication No. 10-2006-0134950), a fused heterocyclic compound (Korean Patent Publication No. 10-2014-0068920), Nitrile compound (Korean Patent Publication No. 10-2010-0125342), fluorine-containing organic sulfur compound (Korea Patent Publication No. 10-2010-0047871), iminopropene compound (Korean Patent Publication No. 10-2008-0072029) ), pyrazine compound (Korean Patent Publication No. 10-2018-0061370), bay, caraway seed (Caraway seed), cardamone Ceylon, lemongrass ( Lemongrass), lime this 5F, oregano, flamarosa, pimento berry, rosewood, thyme red, thyme white, essential oil extracts of wormwood, etc., disclosed in Korean Patent Publication No. 10-2019-0057010 and Codonopsis pilosula (Franch.) Nannf.) extract, and Armeniacae Semen extract disclosed in Korean Patent Publication No. 10-2019-0057011.

In general, vegetable essential oils are known to have pest repelling activity and insecticidal activity (Biores. Technol. 99:2507-2515, 2008). It may be included in the composition together with the active ingredient.

The composition of the present invention may be prepared in any dosage form according to the type of application by a conventional method known in the art, including a solid carrier, a liquid carrier, or a gaseous carrier acceptable in the related art in addition to the active ingredient. Specifically, liquid, emulsion, wettable powder, water soluble powder, water dispersible granule, water soluble granule, suspension, emulsion, powder (dustable powder), granules, tablets, emulsifiable gels, sprays, fumigants, aerosols, smokers, etc. may be prepared in any formulation.

As the solid carrier that can be used in the composition of the present invention, natural minerals, calcined natural materials, inorganic salts, sugars, organic materials, powders of plants, etc. may be used, and specifically, quartz, calcite, sepiolite, dolomite, chalk, kaolinite, Phylophyllite, Cellicite, Halosite, Metahalosite, Wood Grinding Clay, Slotting Clay, Ceramic Stone, Declite, Allopen, Shirasu, Mica, Talc, Bentonite, Activated Clay, Acid Clay, Pumice Stone, Attapulgite , zeolite, diatomaceous earth calcined clay, perlite, shiras balloon, vermiculite, atapulgus clay, calcined diatomaceous earth, magnesium carbonate, calcium carbonate, sodium carbonate, sodium hydrogen carbonate, ammonium sulfate, sodium sulfate, magnesium sulfate, diammonium hydrogen phosphate, ammonium dihydrogen phosphate , potassium chloride, glucose, fructose, sucrose and lactose, starch, powdered cellulose, dextrin, urea, urea derivative, benzoic acid, benzoate salt, wood flour, cork flour, cornstarch, walnut husk, tobacco stem, fly ash, white carbon (such as , hydrous synthetic silica, anhydrous synthetic silica, hydrous synthetic silicate, etc.), fertilizer, and the like can be used.

In addition, as the liquid carrier that can be used in the composition of the present invention, aromatic hydrocarbons, aliphatic hydrocarbons, alcohols, ethers, esters, amides, oils and fats may be used, and specifically, xylene, alkylbenzene, phenylxylylethane , alkylnaphthalene, machine oil, normal paraffin, isoparaffin, naphthene, ethanol, isopropanol, cyclohexanol, phenoxyethanol, benzyl alcohol, ethylene glycol, propylene glycol, diethylene glycol, hexylene glycol, polyethylene glycol, polypropylene glycol , propyl cellosolve, butyl cellosolve, phenyl cellosolve, propylene glycol monomethyl ether, propylene glycol monoethyl ether, propylene glycol monopropyl ether, propylene glycol monobutyl ether, propylene glycol, monophenyl ether, acetophenone, Cyclohexanone, γ-butyrolactone, fatty acid methyl ester, succinic acid dialkyl ester, glutamic acid dialkyl ester, adipic acid dialkyl ester and phthalic acid dialkyl ester, N-alkylpyrrolidone, soybean oil, linseed oil, rapeseed oil, palm oil , cottonseed oil, castor oil, dimethyl sulfoxide, water, and the like can be used.

In addition, as the gaseous carrier that can be used in the composition of the present invention, air, nitrogen, carbon dioxide, dimethyl ether, butane, propane, halogenated hydrocarbons and the like may be exemplified.

The composition of the present invention may further include one or more adjuvants, such as surfactants, dispersants, wetting agents, antifreeze agents, thickeners, preservatives, spreading agents, colorants, and stabilizers, if necessary, in addition to the active ingredient and the carrier component.

As the surfactant, any surfactant such as a nonionic surfactant, an anionic surfactant, or cationic surfactant may be used. Specifically, polyoxyethylene alkyl ether, polyoxyethylene alkylphenyl ether, polyoxyethylene styrylphenyl ether, etc. , polyoxyethylene polyoxypropylene block copolymer, polyoxyethylene fatty acid ester, sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, castor oil ethylene oxide adduct, acetylene glycol, acetylene alcohol, ethylene oxide adduct of acetylene glycol , ethylene oxide adduct of acetylene alcohol, nonionic surfactants such as alkylglycoside, alkyl sulfate ester salt, alkylbenzenesulfonate, ligninsulfonate, alkylsulfosuccinate, naphthalenesulfonate, alkylnaphthalenesulfonate, naphthalenesulfonate Salt of the formalin condensate of phonic acid, the salt of the formalin condensate of alkylnaphthalenesulfonic acid, polyoxyethylene alkyl ether sulfuric acid or phosphoric acid ester salt, polyoxyethylene alkylphenyl ether sulfuric acid or phosphoric acid ester salt, polyoxyethylene styrylphenyl ether sulfuric acid or phosphoric acid Ester salts, polycarboxylates, polystyrene sulfonates, alkylamine salts, alkylammonium salts, silicone-based surfactants, fluorine-based surfactants, and the like may be used.

As the dispersant, nonionic dispersants (ethylene oxide/propylene oxide block polymer, alkylphenol polyglycol ether, tristyrylphenol polyglycol, etc.), anionic dispersants (lignin sulfonic acid, naphthalene sulfonic acid, phenol sulfonic acid, dibutylnaphthalene sulfonic acid, alkylaryl) sulfonate, alkyl sulfate, alkylsulfonate, etc.) or mixtures thereof, and as a wetting agent, alkylnaphthalene sulfonate (especially diisopropylnaphthalenesulfonate or diisobutylnaphthalenesulfonate) can be used, and an antifreeze agent Methanol, ethanol, isopropanol, butanol, glycol, glycerin, diethylene glycol, etc. can be used as the thickener, and as the thickener, cellulose derivatives such as carboxymethyl cellulose, polyacrylic acid derivatives, polyvinylpyrrolidone, carboxyvinyl polymer, high purity bentonite, xanthan Gum, modified clay, xanthan gum, guar gum, diutan gum, starch derivatives, etc. can be used, and dichlorophene, isothiazolene, benzyl alcohol, etc. can be used as preservatives.

In addition, polyvinyl alcohol, polyvinylpyrrolidone, polyacrylate, polymethacrylate, polybutene, polyisobutylene, polystyrene, polyethyleneamine, polyethyleneamide, polyethyleneimine, polyether, etc. can be used as the electrodeposition agent, and a colorant As the inorganic pigments such as iron oxide, titanium oxide, and Prussian blue, organic pigments such as alizarin dye, azo dye, and metal phthalocyanine dye can be used. As the stabilizer, sodium benzoate, potassium sorbate, para-hydroxybenzoic acid Esters, 1,2-benzothiazolin-3-one, preservatives such as formalin solution, cryoprotectants such as polyhydric alcohols such as ethylene glycol, diethylene glycol, propylene glycol, glycerin, starch, alginic acid, mannose, galactose, poly Vinylpyrrolidone, ester gum, freeze inhibitor, zeolite, calcium oxide, magnesium oxide and other drying agents, phenolic antioxidants, amine antioxidants, sulfur antioxidants, phosphoric acid antioxidants, etc. antioxidants, benzophenone ultraviolet absorbers, etc. Decomposition inhibitors, such as a ultraviolet absorber, etc. can be used.

For further details regarding formulation types, carriers, and adjuvants, see "Catalogue of Pesticide Formulation Types and International Coding System" Technical Monograph, 5th Edition by CropLife International (2002), Crop Life International and in Pesticide Specifications, Manual on development and use of FAO and WHO specifications for pesticides, FAO Plant Production and Protection Papers - 173, prepared by the FAO/WHO Joint Meeting on Pesticide Specifications, 2004, ISBN: 9251048576, Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, PA, 1995], literature [Guide to Agrochemical Formulations, Japanese Agrochemical Society/Application Research Society, Published by the Japan Plant Prevention Association, 1997], etc. can be referred to. These documents, together with other documents cited herein, are considered a part of this specification.

In another aspect, the present invention relates to a method for controlling pests using the composition for controlling pests of the present invention as described above.

The pest control method of the present invention comprises the step of contacting the pest control composition of the present invention as described above with pests.

In the step of contacting the composition for controlling pests with pests, the composition for controlling pests of the present invention is sprayed or sprayed on a place where pests inhabit or are distributed, plants such as crops or soil inhabited by plants, or applied to human skin where pests approach. This can be done by

As described above, according to the present invention, it is possible to provide a composition for controlling pests using a nutmeg extract and a method therefor. The composition for controlling pests of the present invention can be used by spraying or spraying on a place where pests inhabit or distribution, plants such as crops or soil inhabited by plants, or applied to human skin where pests approach for pest control.

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. However, the scope of the present invention is not limited to these examples.

< Example > Preparation of Nutmeg Extract

After adding 10 times the weight of methanol to dry powder of nutmeg ( Myristica fragrans ) fruit, leaching at room temperature for 24 hours, filtering with Whatman paper filter paper No. 2, concentration under reduced pressure and freeze-drying to obtain a powdery extract.

< Experimental example > Pest Control Efficacy Test

1. Experimental insects

(1) rice planter

Nillaparvata lugens from the Crop Protection Division of the Rural Development Administration, which had been bred for generations using Oryza sativa seedlings as a host, was used.

For rice seedlings, germinated rice seeds were immersed in a fungicide (bentylate) solution, gauze was laid on an experimental bat (30 X 40 X 8 cm), and the germinated rice seeds were thinly laid out and grown while supplying water in an incubator at 25°C. When the rice seed sprouts grew about 1 cm, they were taken out of the incubator and used while managing in the laboratory. Experimental rice seedling cultivation and seedling cultivation were managed under the conditions of a temperature of 25℃, a relative humidity of 80%, and a photoperiod of 16:8.

(2) aphids

For the breeding of peach aphid ( Myzus persicae Sulzer ), cucumbers ( Cucumis sativus L. white clover) are sown in a plastic pot with a diameter of 7 cm and a height of 7 cm. inoculated. In a breeding room under constant temperature conditions at 25°C, 5-8 plastic pots were placed on a 35 X 25 X 30 cm breeding bed and used for propagation.

(3) Cucumber chrysanthemum

Cucumber thrips ( Thrips plami ) is grown in an acrylic cage (25 X 25 X 30 cm ³ ) was put in and bred. Cucumber leaves are filled with horticultural soil (Baroker, Seoul Bio) in a plastic round pot (Φ8 X 7.5 cm ² ) in a glass greenhouse at 25-30℃, and 2-3 cucumber seeds are sown and the leaves seen after the appearance of cotyledons. -4 When the number of sheets (10cm or more in diameter) was reached, 3 pots were placed in a breeding cage and supplied as food for management.

(4) large-legged house dust mite

The dust mite ( Dermatophagoides farinae ) was managed by storing both feed and a container in which the dust mite was mixed in a plastic container containing saturated saline. The feed was fed by mixing fry feed and dry yeast in a 1:1 ratio, and stirred with a reagent spoon at intervals of 2-3 days to prevent coagulation of the upper layer of the breeding tube so that air was mixed. It was stored and maintained at a temperature of 25±1° C. and a relative humidity of 75±5% under dark conditions.

2. Bioassay

(1) Seedling immersion method (Byeokmyeonggu)

After sowing germinated rice seeds (presumed rice) in the top soil for water supply, young seedlings 3-4 weeks old were removed, washed well, and the stems with roots were cut into 7 cm long and dried in the shade. Five seedling stems as a group were immersed in the prepared solution for 30 seconds, and then dried in the shade at room temperature for at least 30 minutes. The stem treated with the drug was wrapped with gauze soaked in water to include the root, and mounted in a glass test tube (Φ30 X h200 mm). In each glass test tube, 15 nymphs of the 3rd instar nymph were released, and the number of each larvae was investigated after 24 and 48 hours. The assay conditions were maintained at 25±2°C, relative humidity 50-60%, and light conditions 16L:8D, and all tests were repeated three times. The half-lethal concentration (LC ₅₀ ) was calculated by sequentially diluting the sample in distilled water, carrying out the same procedure as the above method, and irradiating the dead larvae.

(2) Leaf immersion method (cucumber thrips and peach aphid)

Cucumber leaf slices with a diameter of 3.5 cm were made and immersed in a beaker containing a chemical solution for 30 seconds. After drying in the shade for 1-2 hours, cotton wool was put on the bottom of the Petri dish and placed on top of the dried cucumber leaves with the back side facing up. Each leaf was irradiated with 15 aphids or adult cucumber thrips, and the number of dead insects was investigated at 25±2℃, 60±5% relative humidity, and 16L:8D light conditions at intervals of 24 hours. All tests were performed in 3 replicates. The half-lethal concentration (LC ₅₀ ) was calculated by sequentially diluting the sample in distilled water, carrying out the same procedure as the above method, and irradiating the dead larvae.

(3) Litter diffusion method (large-legged house dust mite)

The binding part of the lower container of a Petri dish (diameter 5 cm) with a lid was treated with parafilm so that the lid and the lower part could be in close contact, and the paper was prepared by putting it on the bottom. 5 mg of the sample prepared in a 2 ml tube was dissolved in 200 ul of methanol or an appropriate solvent, and the sample was uniformly treated with a pipette on the bottom of the Petri dish, and then dried at room temperature for 2 hours. An appropriate number of adult large-legged house dust mite was sprayed onto the filter paper. It was repeated 3 times and the number of dead insects was investigated after 24 hours and 48 hours under a dissecting microscope (20 times or 40 times). considered).

(4) Statistical processing

According to the primary screening, the semi-lethal concentration (LC ₅₀ ) of the extract with excellent insecticidal power was calculated using probit analysis (SAS Institutem 2004).

3. Results

(1) Hemi-lethal concentration (LC) against ₅₀ )

The half-lethal concentration was calculated by diluting the test substance showing strong insecticidal power at the primary screening concentration of 5,000 ppm by half and immersing the seedlings at 5 concentrations.

As can be seen in Table 1, the LC ₅₀ values of the test substances in the immersion of the seedlings for P. nymph were 1,924 and 1,260 ppm, respectively, after 24 hours and 48 hours.

(2) Half-lethal concentration for cucumber thrips (LC) ₅₀ )

The half-lethal concentration was calculated by diluting the test substance showing strong insecticidal power at the primary screening concentration of 5,000 ppm by half and immersing the leaves at 6 concentrations.

As can be seen in Table 2, the LC ₅₀ values of the test substances in leaf immersion for adults of cucumber thrips were 7,191 and 773 ppm, respectively, after 24 and 48 hours.

(3) Half-lethal concentration for peach aphid (LC) ₅₀ )

The half-lethal concentration was calculated by diluting the test substance showing insecticidal power by half at the primary screening concentration of 5,000 ppm and immersing the leaves at four concentrations.

As can be seen in Table 3, the LC ₅₀ values of the test substance in leaf immersion for adults of P. aphid were 3,130 and 2,691 ppm, respectively, after 24 and 48 hours.

(4) Hemi-lethal concentration (LC) for large-legged house dust mites (LC) ₅₀ )

Six concentrations of test substances that showed excellent insecticidal power in the primary screening were prepared, and the semi-lethal concentration for adult dust mites was calculated.

As can be seen in Table 4, the LC ₅₀ values of the test substances for the adult dust mite were found to be ^{0.11 and 0.10 mg/cm 2} after 24 and 48 hours, respectively.

(5) result synthesis

As a result of 24-hour and 48-hour exposure tests by applying the seedling immersion method, leaf immersion method, and foliar diffusion method suitable for the insecticidal power test for each target pest, the insecticidal power differs according to each pest and the applied test method as shown in Table 5 below. was shown.

Claims (6)

A composition for controlling pests comprising a nutmeg fruit extract as an active ingredient,
The nutmeg fruit extract is an extract using a mixed solvent of an alcohol having 1 to 4 carbon atoms or an alcohol having 1 to 4 carbon atoms and water,
The pest is a peach aphid ( Myzus persicae ) or a large legged house dust mite ( Dermatophagoides farinae ) Composition, characterized in that.
According to claim 1,
The composition comprises a solid carrier, a liquid carrier, or a gaseous carrier.
According to claim 1,
The composition may be a liquid, an emulsion, a wettable powder, a water soluble powder, a water dispersible granule, a water soluble granule, a suspension, an emulsion, and a powder. (dustable powder), granule (granule), tablet (tablet), emulsifiable gel (emulsifiable gel), spray, fumigant, aerosol, and a composition characterized in that the formulation of one of a smoke agent.
According to claim 1,
The alcohol having 1 to 4 carbon atoms is a composition, characterized in that methanol or ethanol.
delete A method for controlling pests comprising the step of contacting the composition of any one of claims 1 to 4 with pests, wherein the pest is Myzus persicae or Dermatophagoides farinae ) Way.