KR20210047099A - Composition and Method for Controling Pests Using an Extract of Myristica fragrans - Google Patents

Abstract

The present invention discloses a composition and method for controlling pests using a Myristica fragrans extract having a controlling effect on pests.

Description

Composition and Method for Controling Pests Using an Extract of Myristica fragrans}

The present invention relates to a composition for controlling pests using an extract of Nutmeg (Myristica fragrans) and a method for controlling the same.

Pests not only directly reduce the yield of crops, but also adversely affect the quality, ultimately leading to economic losses for farmers, so the demand for pesticides for pest control has been steadily increasing for the purpose of increasing crop productivity. Pesticides that have been widely used up to now are organic synthetic pesticides including DDT, and these organic synthetic pesticides have caused problems of biological toxicity due to the food chain of the ecosystem because of high toxicity and low degradability to all pests rather than effective pesticide against specific pests. . In addition, the increase of resistant pests due to the misuse of organic synthetic pesticides, decrease of useful insects, increase of residual pesticides in crops, water quality and soil pollution, etc. are acting as a risk to national health. In order to solve the side effects of these organic synthetic pesticides, destruction of the ecosystem, and environmental pollution, eco-friendly pesticides targeting only the desired pests, which are environmentally safe and target only desired pests, are low toxic to humans and are not harmful to natural enemies because they have high insecticidal effect and low persistence. Efforts to develop are being made.

Among them, eco-friendly insecticides using plant extracts directly use plant extracts that contain the plant's own defense material against insects, nematodes, and plant pathogens, and are environmentally safe, less toxic, and well decomposed.

Tussilagofarfara extract (Seongho Park, Chungnam National University, Master's thesis, 2013), Quisqualis indica extract (Jinsun Song, Kyungpook National University, Master's thesis, 2013), Zanthoxylum Piperitum (Jeongyoon Ko, Chonnam National University, MS Thesis, 2017) Extract, Tanacetum cinerariifolium extract (Korean Patent No. 10-1885083), ivy extract (Korean Patent No. 10-1614095), blackberry seed extract (Korean Patent Laid-Open Patent No. 10-2019 -0032923) have been proposed as eco-friendly insecticides using plant extracts.

The present invention discloses a control activity against pests such as rice sprouts such as nutmeg (Myristica fragrans) extract.

An object of the present invention is to provide a composition for controlling pests using a nutmeg extract.

Another object of the present invention is to provide a method for controlling pests using a nutmeg extract.

Other or specific objects of the present invention will be presented below.

In the present invention, as confirmed in the Examples and Experimental Examples below, nutmeg extract is applied to rice plants (Nilaparvata lugens), peach aphids ( Myzus persicae ), cucumber thrips ( Thrips palmi ), large-legged dust mites ( Dermatophagoides farinae ). It was completed by confirming the insecticidal effect.

Therefore, the composition for controlling pests of the present invention is characterized in that it contains nutmeg extract as an active ingredient.

In the present specification, "nutmeg extract" refers to leaves, stems, above-ground parts, rhizomes, roots, underground parts, or mixtures thereof of nutmeg to be extracted in water, lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.), methylene chloride, Ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof It refers to an extract obtained by leaching by using, an extract obtained by using a supercritical extraction solvent such as carbon dioxide, pentane, or a fraction obtained by fractionating the extract, and the extraction method is cold sedimentation in consideration of the polarity of the active substance, the degree of extraction, and the degree of preservation. Arbitrary methods, such as reflux, warming, ultrasonic radiation, and supercritical extraction, can be applied. In the case of a fractionated extract, it means including a fraction obtained by suspending the extract in a specific solvent and then mixing and policing it with a solvent having a different polarity, and a fraction obtained by column chromatography. In addition, the meaning of the extract includes a concentrated liquid extract or a solid extract from which the extraction solvent has been removed by a method such as freeze drying, vacuum drying, hot air drying, spray drying, or the like. Preferably, it means an extract obtained using water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof as an extraction solvent, particularly an extract obtained using methanol.

In addition, in the present specification, the term "active ingredient" refers to an ingredient capable of exhibiting a desired activity alone or exhibiting activity together with a carrier that is not itself active.

In addition, in the present specification, "pest control" refers to the repelling of pests or insect pests. Here, the pest is meant to include Hemiptera pests, Thysanoptera pests, and Acarina pests. Specifically Hemiptera pests include aemyeolgu (Laodelphax striatellus), Brown Planthopper (Nilaparvata lugens), etc. myeolgugwa (Delphacidae) pests, kkeutdong maemichung (Nephotettix cincticeps), two points kkeutdong maemichung (Nephotettix virescens) maemichung and (Deltocephalidae) pests, cotton aphid, such as ( Aphis gossypii ), Myzus persicae , Brevicoryne brassicae , Macrosiphum euphorbiae , Aulacorthum solani , Toxoptera ciiphum padi , Tangerine aphid ), such as aphids and (Aphididae) pests, pulsaek norinjae (Nezara antennata), the top bridge ant waist norinjae (Riptortus clavetus), leg waist norinjae (Leptocorisa chinensis), visible point round norinjae (Eysarcoris parvus), sseokdeong trees norinjae (Halyomorpha mista ) such as pentatomidae (Pentatomidae) pests, greenhouse whitefly (Trialeurodes vaporariorum), eunip whitefly (Bemisia argentifolii), such as a powder of Science (Aleyrodidae) pests, San Jose pod worm (Comstockaspis perniciosa), arrow pod worm (Unaspis citri), ruby pod worm ( Ceroplastes rubens ) and other Coccidea pests are included, and the total insects of the order of the order of insects include the flower yellow thrips ( Frankliniella occidentalis ), the cucumber thrips ( Thrips palmi ), the convex thrips ( Scirtothrips dorsalis ), and the scallop thrips Thrips tabaci ), Taiwanese thrips ( Frankliniella intonsa ), etc. ipidae) pests, and Acarina pests include Tetranychus urticae and Panonychus citri pests, and Eriophyidae pests such as Aculops pelekassi , primary dust mite (Polyphagotarsonemus latus) include house dust jindeugigwa (Pyroglyphidae) such as dust, insects eungaegwa (Tarsonemidae) pest, a large bridge house dust mite (Dermatophagoides farinae), house dust mite vertical pattern (Dermatophagoides pteronyssinus) and the like. Preferably, in the following examples, pesticides and other control activities have been confirmed ( Nilaparvata lugens ), peach aphids ( Myzus persicae ), cucumber thrips ( Thrips palmi ), large-legged dust mites ( Dermatophagoides farinae ).

In the composition of the present invention, the active ingredient may be included in an arbitrary amount (effective amount) according to the specific control use, formulation, etc., as long as it can exhibit pest control activity, etc., and the usual effective amount is 0.001 based on the total weight of the composition. It will be determined within the range of weight% to 20.0 weight %. Here, "effective amount" refers to the amount of the active ingredient contained in the composition of the present invention, which can exhibit a pest control effect. Such effective amounts can be determined empirically within the range of ordinary skill in the art.

In addition to the active ingredient, the composition of the present invention may contain any compound or natural product-derived material known to have pest control activity in the art for synergistic and reinforcement of pest control activity. Such compounds or substances derived from natural products are tetrazolinone compounds (

Korean Patent Laid-Open Patent No. 10-2015-0008863), halogen-containing organosulfur compounds (Korean Laid-Open Patent No. 10-2010-0031518), alpha-alkoxyphenyl acetic acid derivatives (Korean Laid-Open Patent No. 10-2011-0057160), Pyrimidine compound (Korean Laid-Open Patent No. 10-2006-0009324), nitrile compound (Korean Laid-Open Patent No. 10-2006-0134950), fused heterocyclic compound (Korean Laid-Open Patent No. 10-2014-0068920), Nitrile compounds (Korean Laid-Open Patent No. 10-2010-0125342), fluorine-containing organosulfur compounds (Korean Laid-Open Patent No. 10-2010-0047871), iminopropene compounds (Korean Laid-Open Patent No. 10-2008-0072029) ), pyrazine compound (Korean Patent Laid-Open Patent No. 10-2018-0061370), Bay disclosed by Korean Patent Laid-Open Patent No. 10-2006-0100743, Caraway seed, Cardamone Ceylon, Lemongrass ( Lemongrass), Lime Dis 5F, Oregano, Plamarosa, Pimento Berry, Rosewood, Thyme Red, Thyme White, and essential oil extracts such as Wormwood, disclosed by Korean Patent Laid-Open No. 10-2019-0057010 Mansam (Codonopsis pilosula (Franch.) Nannf.) extract, Korean Patent Application Laid-Open No. 10-2019-0057011 discloses an extract of Armeniacae Semen, and the like.

In general, vegetable essential oils are known to have pest repelling activity and insecticidal activity (Biores. Technol. 99:2507-2515, 2008), and any plant essential oil other than the above-exemplified essential oils is also of the present invention as long as it has pest control activity. It may be included in the composition together with the active ingredient.

The composition of the present invention may be prepared in any dosage form according to the type of application by a conventional method known in the art, including a solid carrier, a liquid carrier, or a gaseous carrier acceptable in the related art in addition to the active ingredient. Specifically, liquids, emulsions, wettable powders, water soluble powders, water dispersible granules, water soluble granules, suspensions, emulsions, powders (dustable powder), granules, tablets, emulsifiable gels, sprays, fumigants, aerosols, smoke screens, etc. can be prepared in any formulation.

As the solid carrier that can be used in the composition of the present invention, natural minerals, calcined natural minerals, inorganic salts, sugars, organic substances, powders of plants, etc. can be used. Specifically, quartz, calcite, meerschaum, dolomite, chalk, kaolinite, Pylophilite, celicite, halosite, metahalosite, wooden clay, wamok clay, pottery stone, declite, allophene, shirasu, mica, talc, bentonite, activated clay, acid clay, pumice, attapulgite , Zeolite, diatomaceous earth calcined clay, pearlite, shirasu balloon, vermiculite, atapulgus clay, calcined diatomaceous earth, magnesium carbonate, calcium carbonate, sodium carbonate, sodium hydrogen carbonate, ammonium sulfate, sodium sulfate, magnesium sulfate, diammonium hydrogen phosphate, ammonium dihydrogen phosphate , Potassium chloride, glucose, fructose, sucrose and lactose, starch, powdered cellulose, dextrin, urea, urea derivatives, benzoic acid, benzoate, wood powder, cork powder, axilla, walnut shell, tobacco stem, fly ash, white carbon (e.g. , Hydrous synthetic silica, anhydrous synthetic silica, hydrous synthetic silicate, etc.), fertilizers, and the like may be used.

In addition, as a liquid carrier that can be used in the composition of the present invention, aromatic hydrocarbons, aliphatic hydrocarbons, alcohols, ethers, esters, amides, fats and oils may be used. Specifically, xylene, alkylbenzene, phenylxylylethane , Alkylnaphthalene, machine oil, normal paraffin, isoparaffin, naphthene, ethanol, isopropanol, cyclohexanol, phenoxyethanol, benzyl alcohol, ethylene glycol, propylene glycol, diethylene glycol, hexylene glycol, polyethylene glycol, polypropylene glycol , Propyl cellosolve, butyl cellosolve, phenyl cellosolve, propylene glycol monomethyl ether, propylene glycol monoethyl ether, propylene glycol monopropyl ether, propylene glycol monobutyl ether, propylene glycol, monophenyl ether, acetophenone, Cyclohexanone, γ-butyrolactone, fatty acid methyl ester, succinic acid dialkyl ester, glutamic acid dialkyl ester, adipic acid dialkyl ester and phthalic acid dialkyl ester, N-alkylpyrrolidone, soybean oil, flaxseed oil, rapeseed oil, palm oil , Cottonseed oil, castor oil, dimethyl sulfoxide, water, etc. may be used.

Further, examples of the gaseous carrier that can be used in the composition of the present invention include air, nitrogen, carbon dioxide, dimethyl ether, butane, propane, halogenated hydrocarbons, and the like.

In addition to the active ingredient and the carrier component, the composition of the present invention may further include one or more auxiliary agents such as surfactants, dispersants, wetting agents, antifreeze agents, thickeners, preservatives, electrodeposition agents, colorants, and stabilizers, if necessary.

As the surfactant, any surfactant such as nonionic surfactant, anionic surfactant, cationic surfactant, etc. can be used. Specifically, polyoxyethylene alkyl ether, polyoxyethylene alkylphenyl ether, polyoxyethylene styrylphenyl ether , Polyoxyethylene polyoxypropylene block copolymer, polyoxyethylene fatty acid ester, sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, castor oil ethylene oxide adduct, acetylene glycol, acetylene alcohol, ethylene oxide adduct of acetylene glycol , Ethylene oxide adduct of acetylene alcohol, nonionic surfactants such as alkyl glycosides, alkyl sulfuric acid ester salts, alkylbenzene sulfonates, lignin sulfonates, alkyl sulfosuccinates, naphthalene sulfonates, alkyl naphthalene sulfonates, naphthalene sulfonates Salts of formalin condensate of phonic acid, salt of formalin condensation of alkylnaphthalenesulfonic acid, polyoxyethylene alkyl ether sulfuric acid or phosphate ester salt, polyoxyethylene alkylphenyl ether sulfuric acid or phosphate ester salt, polyoxyethylene styrylphenyl ether sulfuric acid or phosphoric acid Ester salts, polycarboxylic acid salts, polystyrene sulfonates, alkylamine salts, alkyl ammonium salts, silicone-based surfactants, fluorine-based surfactants, and the like can be used.

In addition, as dispersants, nonionic dispersants (ethylene oxide/propylene oxide block polymer, alkylphenol polyglycol ether, tristyrylphenol polyglycol, etc.), anionic dispersants (ligninsulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, dibutylnaphthalenesulfonic acid, alkylaryl) Sulfonate, alkyl sulfate, alkyl sulfonate, etc.) or mixtures thereof, and as wetting agent, alkyl naphthalene sulfonate (especially diisopropyl naphthalene sulfonate or diisobutyl naphthalene sulfonate) can be used, and antifreeze Methanol, ethanol, isopropanol, butanol, glycol, glycerin, diethylene glycol, and the like can be used as a thickener, and as a thickener, cellulose derivatives such as carboxymethylcellulose, polyacrylic acid derivatives, polyvinylpyrrolidone, carboxyvinyl polymer, high purity bentonite, xanthan Gum, modified clay, xanthan gum, guar gum, diutan gum, starch derivatives, and the like can be used, and as preservatives, dichlorophene, isothiazolene, benzyl alcohol, and the like can be used.

In addition, as the electrodeposition agent, polyvinyl alcohol, polyvinylpyrrolidone, polyacrylate, polymethacrylate, polybutene, polyisobutylene, polystyrene, polyethyleneamine, polyethyleneamide, polyethyleneimine, polyether, etc. can be used. As examples, inorganic pigments such as iron oxide, titanium oxide, and Prussian blue, and organic pigments such as alizarin dye, azo dye, and metal phthalocyanine dye can be used. As a stabilizer, sodium benzoate, potassium sorbate, and para-hydroxybenzoic acid Preservatives such as ester, 1,2-benzothiazolin-3-one, formalin solution, freeze-removing agents such as polyhydric alcohols such as ethylene glycol, diethylene glycol, propylene glycol, and glycerin, starch, alginic acid, mannose, galactose, poly Vinylpyrrolidone, ester gum, freeze inhibitor, zeolite, calcium oxide, drying agents such as magnesium oxide, phenolic antioxidants, amine antioxidants, sulfur antioxidants, antioxidants such as phosphoric acid antioxidants, benzophenone UV absorbers, etc. Decomposition inhibitors, such as an ultraviolet absorber, can be used.

For more specific information regarding the type of formulation, carrier, and adjuvant, see "Catalogue of Pesticide Formulation Types and International Coding System" Technical Monograph, 5th Edition by CropLife International (2002), Crop Life International and in Pesticide Specifications, Manual on development and use of FAO and WHO specifications for pesticides, FAO Plant Production and Protection Papers-173, prepared by the FAO/WHO Joint Meeting on Pesticide Specifications, 2004, ISBN: 9251048576, Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, PA, 1995], literature [Pesticide Formulation Guide, Japanese Society for Pesticide Application and Method Research, published by Japan Plant Protection Association, 1997], etc. can be referenced. These documents, along with other documents cited herein, are considered part of this specification.

In another aspect, the present invention relates to a pest control method using the pest control composition of the present invention as described above.

The pest control method of the present invention comprises the step of contacting the pest control composition of the present invention as described above with the pest.

In the step of bringing the pest control composition into contact with the pest, the pest control composition of the present invention is sprayed and sprayed onto the soil in which the pests live or distribute, plants, such as crops, or the human skin that the pests approach. It can be done by doing.

As described above, according to the present invention, it is possible to provide a composition and method for controlling pests using a nutmeg extract. The composition for controlling pests of the present invention may be sprayed and sprayed on a place where pests inhabit or distribute, or plants such as crops or soil inhabited by plants for pest control, or applied to human skin to which pests approach.

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. However, the scope of the present invention is not limited to these examples.

< Example > Preparation of nutmeg extract

Nutmeg ( Myristica fragrans ) 10 times the weight of methanol was added to the dried fruit powder, leached at room temperature for 24 hours, filtered through Whatman paper filter paper No.2, concentrated under reduced pressure, and freeze-dried to obtain a powdery extract.

< Experimental example > Pest control efficacy test

1. Experimental insects

(1) thunderbolt

Rice seedlings (Nilaparvata lugens ) sold by the Crop Protection Division of the Rural Development Administration, which have been reared using Oryza sativa seedlings as the host, were used.

Rice seedlings were cultivated by immersing rice seeds in a disinfectant (ventilate) solution, spreading gauze on the experimental baht (30 X 40 X 8 cm), spreading the germinated rice seeds thinly, and supplying water in an incubator at 25°C. When rice seed sprouts grew about 1cm, they were taken out of the incubator and used while managing in the laboratory. Experimental rice plant breeding and seedling cultivation were managed under the conditions of a temperature of 25℃, a relative humidity of 80%, and a photoperiod of 16:8.

(2) aphids

To breed the peach aphid ( Myzus persicae Sulzer ), sowing cucumbers (Cucumis sativus L. white tea) in a plastic pot with a diameter of 7 cm and a height of 7 cm, and after 30 days, cotton aphids were planted on the cucumbers with 1-2 sheets of the main leaf. Inoculated. 5-8 plastic pots were put on a 35 X 25 X 30 cm breeding room in a breeding room under constant temperature conditions at 25°C to grow and use.

(3) Cucumber side dish

Cucumber thrips plami (Thrips plami ) is an acrylic cage (25 X 25) with a pot of cucumber (Baekda Dagi, Heungnong Seedling Co., Ltd.) in the breeding room (temperature: 25±2℃, relative humidity: 60±5%, photoperiod: 16L:8D). X 30 cm ³ ) and reared. Cucumber leaves are filled with garden soil (baroker, Seoul Bio) in a plastic circular pot (Φ8 X 7.5 cm ^{2) in a glass greenhouse at 25-30℃, and 2-3 cucumber seeds are sown.} -When it reached 4 sheets (more than 10cm in diameter), it was managed by feeding it into 3 pots each in a breeding cage.

(4) Large leg house dust mite

Dermatophagoides farinae (Dermatophagoides farinae) was managed by storing a container containing feed and large-legged dust mite in a plastic container containing saturated saline. The fry feed and dry yeast were mixed in a 1:1 ratio and fed, and the upper layer of the breeding tank was stirred with a reagent spoon at intervals of 2-3 days to prevent coagulation to allow air to be mixed. It was kept at a temperature of 25±1°C and a relative humidity of 75±5% under dark conditions.

2. Bioassay

(1) Seedling soaking method

The germinated rice seed (chujeong rice) was sown in the upper soil for water, and then 3-4 week old young seedlings were pulled out and washed well, and the stems were cut into 7 cm long with roots and dried in the shade. Five seedling stems were immersed in the prepared liquid solution as a group for 30 seconds, and then dried in the shade for 30 minutes or more at room temperature. The stem treated with the drug was wrapped with water-soaked gauze to include the root and mounted in a glass test tube (Φ30 X h200 mm). In each glass test tube, 15 larvae of 3 instars were radiated, and the number of larvae was investigated after 24 and 48 hours. The assay conditions were maintained at 25±2°C, 50-60% relative humidity, and 16L:8D light conditions, and all tests were carried out in 3 repetitions. The half-lethal concentration (LC ₅₀ ) was calculated by sequentially diluting the sample in distilled water, performing the same as the above method, and irradiating dead insect water.

(2) Leaf immersion method (cucumber gunworm and peach aphid)

Cucumber leaf slices with a diameter of 3.5 cm were made and immersed in a beaker containing the medicinal solution for 30 seconds. After drying for 1-2 hours, put cotton wool on the bottom of the petri dish and put the back side of the dried cucumber leaves on top of it. Fifteen aphids or adult cucumber thrips per leaf were irradiated, respectively, and the number of dead insects was irradiated at 24 hour intervals at a temperature of 25±2℃, a relative humidity of 60±5%, and a light condition of 16L:8D. All tests were carried out in 3 repetitions. The half-lethal concentration (LC ₅₀ ) was calculated by sequentially diluting the sample in distilled water, performing the same as the above method, and irradiating dead insect water.

(3) Litigation spreading method (large leg house dust mite)

The binding part of the lower container of the petri dish (diameter 5 cm) with a lid was treated with parafilm so that the lid and the lower part were in close contact, and a filter paper was put on the floor to prepare. 5 mg of a sample prepared in a 2 ml tube was dissolved in 200 ul of methanol or an appropriate solvent, and uniformly treated in a filter area at the bottom of the Petri dish prepared with a pipette, and dried at room temperature for 2 hours. An appropriate number of adult mites of large-legged house dust mites was radiated onto the filter paper. It was repeated 3 times, and the number of dead insects was investigated after 24 hours and 48 hours under a dissecting microscope (20 times or 40 times). Regarded as).

(4) Statistical processing

According to the first screening, the half-lethal concentration (LC ₅₀ ) for the extract having excellent insecticidal power was calculated using probit analysis (SAS Institutem 2004).

3. Results

(1) Half-lethal concentration (LC ₅₀ )

At the first screening concentration of 5,000 ppm, the test substance showing strong insecticidal power was diluted by half, and seedlings were immersed in 5 concentrations to calculate the half-lethal concentration.

As can be seen in Table 1, the LC ₅₀ values of the test substances in the seedling immersion in the nymphis nymph were 1,924 and 1,260 ppm, respectively, after 24 hours and 48 hours.

(2) Half-lethal concentration for cucumber thrips (LC ₅₀ )

At the first screening concentration of 5,000 ppm, the test substance that showed strong insecticidal power was diluted by half, and the leaves were immersed in 6 concentrations to calculate the half-lethal concentration.

_{As confirmed in Table 2, the LC 50} value of the test substance in leaf immersion for adult cucumber thrips was 7,191 and 773 ppm after 24 hours and 48 hours, respectively.

(3) Half-lethal concentration (LC ₅₀ )

At the first screening concentration of 5,000 ppm, the test substance showing insecticidal power was diluted by half, and the leaves were immersed in 4 concentrations to calculate the half-lethal concentration.

_{As confirmed in Table 3, the LC 50} value of the test substance in leaf immersion for adult peach aphid was 3,130 and 2,691 ppm after 24 hours and 48 hours, respectively.

(4) Half-lethal concentration (LC ₅₀ )

In the first screening, test substances showing excellent insecticidal power were prepared in 6 concentrations to calculate the half-lethal concentration for adult large-legged dust mites.

_{As can be seen in Table 4, the LC 50} value of the test substance for adult large-legged dust mites ^{was 0.11 and 0.10 mg/cm 2} , respectively, after 24 and 48 hours.

(5) Synthesis of results

As a result of 24-hour and 48-hour exposure tests by applying the seedling soaking method, leaf soaking method, and litter diffusion method suitable for the insecticidal test for each target pest, as shown in Table 5 below, the insecticidal power differs for each pest and according to the applied test method. Is shown.

Claims (6)

A composition for controlling pests comprising nutmeg extract as an active ingredient.
The method of claim 1,
The pests are Norinjaemok (Hemiptera) pests, Thysanoptera pests, tick tree (Acarina) pests composition.
The method of claim 1,
The pests myeolgugwa (Delphacidae) insects, aphids and (Aphididae) pests, total beolregwa (Thripidae) insects or dust jindeugigwa (Pyroglyphidae) pest composition.
The method of claim 1,
The pest is a rice plant (Nilaparvata lugens), peach aphid ( Myzus persicae ), cucumber thrips ( Thrips palmi ) or large-legged dust mite ( Dermatophagoides farinae ) composition.
The method of claim 1,
The composition, characterized in that the extract is water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent extract thereof.
A pest control method comprising the step of contacting the composition of any one of claims 1 to 5 with a pest.