KR101920538B1 - Insecitcide Composition with the Extract of Tanacetum cinerariifolium, and Agastache rugosa - Google Patents

Abstract

The present invention relates to an insecticidal composition comprising an insect repellent and an extract from a pine needle as an active ingredient.
The insecticidal composition of the present invention has an excellent insecticidal effect against insect pests by maximizing the insecticidal effect of the insect pest by the addition of the pesticide. Therefore, the pesticide composition of the present invention can be usefully used as an environmentally friendly material for agricultural pests and hygiene pests.
Thus, the pesticide composition of the present invention can be usefully used as an effective environmentally friendly insecticide material to replace chemical synthetic pesticides in the control of agricultural pests and hygiene pests.

Description

[0001] The present invention relates to an insecticidal composition comprising an insect repellent composition and an extract of Pseudomonas sp.

The present invention relates to an insecticidal composition comprising a plant mixed extract as an active ingredient, and more particularly to an insecticidal composition containing an insecticidal composition containing an insect repellent and an extract of a pine needle as an active ingredient as an active ingredient.

Pesticides are drugs that kill insects that harm humans and crops. Demand for the use of insecticides, which are used to control insects and improve the productivity of crops, is continuously maintained.

Chemical synthetic pesticides which have been used conventionally have contributed greatly to increase agricultural productivity by controlling disease or insect pests caused by cultivation of crops. However, excessive use of chemically synthesized insecticides has caused pollution of soil and water quality, and it has become a threat to the health of farmers by polluting environment of facilities. In addition, residual chemical components of agricultural products grown using chemical synthetic insecticides are very toxic to humans, so consumers prefer safe foodstuffs grown without synthetic pesticides. In recent years, concerns about the safety of agricultural products and the concern for sustainable agriculture through conservation of soil environment have been increasing. In this environment, organic agriculture is rapidly spreading throughout the world, and household insecticides, insect repellents, Pesticides and pesticides for pesticides are also common.

In addition, natural insecticides used in organic farming are harmless to human beings, livestock, fish and shellfish, and there is no fear of environmental pollution and ecosystem disturbance. The use of natural insecticides using plant extracts has the advantage of being able to preserve the soil environment continuously and to ensure the safety by eliminating the risk of pesticide residue on the crops.

Pesticides can be applied to the treated plant by applying insecticide to the soil in the form of a residual insecticide with a local effect depending on how the insecticide is applied and dispersed in the soil and the systemic insecticide ).

Examples of the insecticides contained in plants and minerals extracted from pest insects include pyrethrins (extracted from bugs), rotheneses (extracted from roots of Deeris), nicotine (extracted from tobacco) (Separated from monument) are widely known. Related patents include registered patent No. 10-1221735 entitled " Natural Pesticide Composition Containing Rhodiola Extract and Clover Extract as Active Ingredient ", Registered Patent No. 1016200020000 " Natural Pesticide Composition & Registered patent No. 1014827640000 " Natural insecticide composition containing plant extract as an active ingredient ".

However, the natural insecticides known so far have a merit that they have less side effects on the human body and the environment than chemically synthesized insecticides. On the other hand, the insecticidal effect is low and it is difficult to obtain a rapid effect. The disadvantage that the amount of water is reduced and the cost is increased if it is used at a high concentration.

On the other hand, Insect flower (Pyrethrum, Dalmatian pyrethrum) is a perennial herbaceous plant of Cercopithecidae. Pyrethrins is a pale yellow-yellow oily substance. Pyretrin is a safe, naturally occurring insecticide that is toxic to warm-blooded animals such as humans, although it exhibits strong insecticidal power by paralyzing cold-blooded animals, particularly insects. Specifically, pyrethrin has strong insecticidal action against insects, and it is used for mosquitoes and flies as a coir type mosquito repellent in addition to emulsion, and is also applicable to cockroaches, fleas, ants and similar crawling insects And is used as a crop protection agent in combination with other natural pesticide ingredients.

Despite the fact that the pest control extract is 17 times safer than caffeine and has excellent insecticidal effects and is approved as an environmentally friendly pesticide by the World Organic Agriculture Federation (IFOAM) and the World Food Standards Committee (CODEX) Due to the economic problems that arise from the fact that only relatively expensive phytophagous flower parts should be extracted and used, and the problem of supply and demand of raw materials which are difficult to secure enough phytophthora flowers as a pesticide raw material, the development of insecticides mainly composed of pest- There has been a difficult problem, and until now, a bug bug has been used as an auxiliary ingredient to be mixed with other plant natural pesticide ingredients.

Accordingly, the present inventors have made efforts to overcome the problems of the prior arts, to find natural ingredients that help to maximize the insecticidal effect of the insecticide, and to increase the control effect of the natural insecticide through it. As a result, The present inventors have confirmed that the insecticidal effect of the insecticide is maximized when the insecticidal composition is prepared in combination and sprayed in spray form.

Accordingly, it is a main object of the present invention to provide an insecticidal composition containing a pest control agent and a pesticidal extract as an active ingredient.

Another object of the present invention is to provide a method for insect pest control by spraying the pesticide composition.

According to one aspect of the invention there is pyrethrum (Tanacetum cinerariifolium ) and blastocyst ( Agastache The present invention provides an insecticidal composition comprising, as an active ingredient, an insecticidal composition containing, as an active ingredient,

The insecticidal composition using the compound plant extract of the present invention corresponds to a commercially available plant extract base. The inventors have directly verified through experiments that the composition of the present invention exhibits a high insecticidal effect on the Dicotyledonous insects, especially mushroom flies, and at the same time has a high insecticidal effect on the insect pests such as ants.

In the present invention, the insecticidal composition and the insecticide have the same meaning unless otherwise stated.

In the present invention, the term " Tanacetum cinerariifolium "refers to a perennial plant of the dicotyledonous plant, and contains an insecticidal component called pyrethrins, which has a chemical structure of pyrethroid. The flower of the insecticide is pyrethrin Pyrrhotin is the most abundant part of the plant, and contains 10 to 20 times more pyrretrin than the flower bud, stem and leaf parts. It is used as the main ingredient of the insecticide composition of the present invention. It is preferred to collect and dry the full bug bug in the pesticide composition of the present invention, which is extracted most at full bloom.

The term " bamboo grass (eucalyptus, Agastache rugosa "refers to a perennial plant belonging to the family Lamiaceae. It is a native plant with a strong growth potential in the mountainous area which is formed after the colonization, and is distributed in Northeast Asia. In Korea, it is mainly grown in the southern part, In the present invention, the bamboo flies themselves have little or no insecticidal effect, but when used in combination with a bug bug, they act as a kind of effect enhancer that maximizes the insecticidal effect possessed by the bug bug. do.

The term " extract " used in the present invention refers to an extract obtained by extracting a plant, a diluted solution or concentrate of the extract, a dried product obtained by drying the extract, a controlled preparation or a purified product of the extracted solution, Extracts themselves and extracts of all formulations which can be formed using extracts.

The pest control and pest control extracts of the present invention can be extracted from natural, hybrid, or variant plants of the pest control and pest control. Preferably, the bug extract extract is extracted from a flower part. The bug extract may also be extracted from a plant tissue culture, and includes extracts extracted from leaves, stems, roots, flowers, fruits and the like.

The insect repellent composition of the present invention can be extracted by a known extraction method, and the method is not limited thereto. For example, the extract may be obtained by washing, drying and pulverizing the insect repellant and the bamboo shoot extract; And the pulverized material may be extracted with a solvent selected from water, an organic solvent or a mixed solvent thereof, and may be extracted by various methods such as distillation extraction and supercritical extraction.

In addition, the mixed extract of the present invention may be a mixed extract obtained by extracting each of the insect repellent and foliar extracts and a combination of the respective extracts produced, and the mixed extract obtained by collectively extracting the mixture of the insect repellent and the foliar extract may be used.

In the insecticidal composition of the present invention, the extract is preferably extracted with an organic solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, and mixed solvents thereof.

The extract can be extracted using water or an organic solvent, and the kind of the organic solvent is not limited. For example, those extracted with a solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, and mixed solvents thereof, preferably methanol, ethanol or butanol. In the present invention, the above-mentioned extract comprises any one of an extract obtained by an extraction treatment, a diluted solution or a concentrate of the extract, a dried product obtained by drying the extract, or any of these adjusted products or purified products. In addition, the extraction method is not particularly limited, and extraction can be carried out at room temperature or with heating under conditions where the active ingredient is not destroyed or minimized.

In the insecticidal composition of the present invention, it is preferable that the mixed extract of the insect repellent and the pine needle are mixed in a weight ratio of 1: 5-30. When mixed within the above-mentioned ratio range, the spectrum of insect pest to be controlled is broadened, And the effect of the insecticidal effect of the main insecticide is maximized.

In addition, the insecticidal composition of the present invention usually contains 0.01 to 95% by weight of the combined extract of the insect repellent and the pomegranate as the active ingredient.

In the pesticide composition of the present invention, the insecticidal object of the composition is not limited and may include agricultural pests, hygiene pests and forest pests, and may include complete pests and incomplete pests. In addition, the insecticidal object includes both adults and larvae. Examples of the insecticidal object include, for example, Lepidoptera, Coleoptera, Homoptera, Hemiptera, Thysanoptera, Isoptera, Orthoptera, (Diptera), Hymenoptera, Siphonaptera, Acarina, and the like, and can be used in agricultural and forestry fields such as mushroom flies, ants, aphids, cabbage worms, There are many kinds of pests in the world.

When the insecticidal composition of the present invention is used as an active ingredient of an insecticide, it can be used as such, without adding any other ingredients other than the mixed extract of the insecticide and the syrup.

The insecticidal composition of the present invention may be mixed with a conventional solid carrier, a liquid carrier, a gas carrier or a bait, or absorbed in a basic substance such as a porous ceramic plate or a nonwoven fabric, Active agent and, if desired, other adjuvants, may be added to formulate the active ingredient in a variety of formulations such as suspensions, suspoemulsions, wettable powders, granular concentrates, microemulsions, wettable powders, granules, powders, emulsions, And one or more formulations selected from the group consisting of gels, and the like.

Particularly, it is preferable that the pesticide composition of the present invention has a formulation in the form of a softening agent in which carbon dioxide gas is dispersed as a carrier. When carbon dioxide gas is formulated as a carrier, the carbon dioxide gas used as a carrier accelerates the respiratory action of the plant, thereby helping the growth of the plant. Through the spraying method of the smoke type, It is present in the form of smoke on the floor of the inside, and it is injected into the body directly through the respiratory as well as the skin of the pest, and the insecticidal effect is increased by about 3 to 5 times.

Methods for spraying insecticides in spray form using carbon dioxide gas as a carrier are well known in the art and fume containing insecticide compositions can be generated by simply contacting the insecticide composition with solid carbon dioxide. In addition, a gas pipe for submerging solid carbon dioxide or a discharge pipe through which carbon dioxide gas is discharged can be generated in a container carrying the insecticide composition to generate fumes containing the insecticidal composition, and the patent related to the carbon dioxide supply device or the carbonic acid gas supply There is a patent document 10-2010-0125499 or a patent document 10-2011-0013608.

Examples of the gas carrier or propellant that can be used in addition to the carbon dioxide gas include freon gas, butane gas, LPG (liquefied petroleum gas), dimethyl ether, and the like.

Solid carriers that can be used in the pesticide compositions of the present invention include fine powders or granules of clay materials such as kaolin clay, diatomaceous earth, synthetic hydrated silicon oxide, bentonite, clay and clay; Various talc, ceramics and other inorganic materials such as sericite, quartz, sulfur, activated carbon, calcium carbonate and hydrated silica; And chemical fertilizers such as ammonium sulphate, ammonium phosphate, ammonium nitrate, urea and ammonium chloride.

The liquid carrier may include water, alcohols such as methanol and ethanol, ketones such as acetone and methyl ethyl ketone, aromatic hydrocarbons such as benzene, toluene, xylene, ethylbenzene and methylnaphthalene, aliphatic Esters such as ethyl acetate and butyl acetate, nitriles such as acetonitrile and isobutyrnitrile, ethers such as diisobutyl ketone, Propyl ether and dioxane, acid amides such as N, N-dimethylformamide and N, N-dimethylacetamide, halogenated hydrocarbons such as dichloromethane, trichloroethane and carbon tetrachloride, Vegetable oils such as soybean oil and cottonseed oil.

The surfactant may include alkyl sulfates, alkyl sulfonates, alkylaryl sulfonates, alkylaryl ethers and polyoxyethylene derivatives thereof, polyethylene glycol ethers, polyhydric alcohol esters and sugar alcohol derivatives.

Adjuvants such as the adhesive or dispersing agent include, but are not limited to casein, gelatin, polysaccharides such as starch, gum arabic, cellulose derivatives and alginic acid, lignin derivatives, bentonites, sugars and synthetic water-soluble polymers such as polyvinyl alcohol, Pyrrolidone, and polyacrylic acid.

Examples of the stabilizer include PAT (isopropyl acid phosphate), BHT (2,6-di-tert-butyl-4-methylphenol), BHA (2-tert- -4-methoxyphenol), vegetable oils, mineral oils, surfactants, fatty acids and esters thereof.

When the pesticide composition of the present invention is used as an insecticide, the amount thereof is usually 10 to 1000 g per 10 acres. In the case of a spray agent using carbon dioxide as a gas carrier, an emulsifiable concentrate to be used after dilution with water, a wettable powder, a milk animal and other similar agents, the application concentration is usually in the range of 1 to 100,000 ppm. Application of granules, dusts or other like formulations is carried out in a non-diluted formulation. Such application amount and concentration may vary depending on the form of the preparation, the time and place of application, the place and method, the type of pest, the degree of damage, and other factors.

In addition, since the pest has a natural defensive system that prevents water from penetrating into the body by allowing water droplets to flow by using surface tension when a liquid component comes into contact with the surface thereof, the insecticide composition May be mixed with a penetrating agent.

The pesticide composition may be diluted to an appropriate concentration at the time of use.

The pest control method is applicable to a method of directly spraying, spraying or applying a composition for controlling insect pests inside or around a building in which crops and / or soil or hygienic insects in a facility house reside.

Further, according to another aspect of the invention there is pyrethrum (Tanacetum preparing an aqueous solution containing cinerariifolium and an extract of Agastache rugosa ; Contacting the prepared aqueous solution with carbon dioxide gas sublimated in dry ice to produce a mixed gas; And treating the mixed gas to insect pests.

In a specific embodiment, the insecticidal method of the present invention is applied to a mixture of the insecticidal composition and carbon dioxide of the present invention in consideration of various factors such as the formulation of the insecticide composition, the kind of insect pest to be insected, In a preferred embodiment, the method includes treating the insecticidal composition with the carbon dioxide gas mixture to an insect pest or habitat in an effective amount.

The insecticidal method of the present invention is produced by an insecticidal composition comprising the insecticide extract of the present invention and the extract of the present invention as an active ingredient and the contents common to both of them are used in order to avoid the excessive complexity of the specification according to the repetitive description, .

As described above, the insecticidal composition according to the present invention maximizes the insecticidal effect of the insect pest by virtue of the addition of the insecticidal insecticide, and thus the pest insecticidal effect is excellent. Therefore, the pesticide composition of the present invention can be usefully used as an environmentally friendly material for agricultural pests and hygiene pests.

In particular, since the plant extract of the present invention is a substance extracted from a plant, it has an advantage of being environmentally friendly for pest control, and it has no resistance problem because it has a mechanism of action different from the existing chemically synthesized pesticide.

Fig. 1 is a photograph of a scene in which a pesticide composition of the present invention is sprayed on a crop in a fog form.
Fig. 2 is a photograph showing a scene where an effect of the insecticidal composition of the present invention is tested on mushroom flies.
FIG. 3 is a graph showing the results of treating the mushroom flies with a diluent of the insecticidal composition of the present invention, respectively.
Figure 4 is a graph showing the results of treating the ants with the diluent of the pesticide composition of the present invention, respectively.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example  1. Preparation of plant extracts

5 kg of dried flower cultivated and harvested from Dalmatian pest control farm in Muchongri, Cheonan, Gwangdeok-myeon, Cheonan was extracted by dipping in 25 L of fermented alcohol (95% ethanol) for 5 days.

Seed purchased from Arah Jongmyo was sowed in April 2016 in a greenhouse in Moohalli, Gwangdeok-myeon, Cheonan, and 5 kg of the herbal extracts collected in July, 2016 were extracted by immersing in 10 L of fermented sake (95% ethanol) for 5 days .

The insecticidal composition containing the insect repellent and the extract of the present invention as an active ingredient of the present invention was prepared by diluting each of the extracted insect repellent extract and the sphagnum extract with distilled water according to the dilution ratio shown in Table 1 below and mixing the diluted extract with 100 mL. Respectively.

[Table 1]

Example  2. Mixture of plant extracts Insecticide  Experiment

2-1 Mushroom flies control

In order to examine the insecticidal rate of the mushroom flies of the extract prepared in Example 1, insecticidal test was carried out in a laboratory.

Mushroom flies were collected from a mushroom cultivator with mushroom flies. The mushroom flies were fed with sawdust medium in which mushroom mycelium was cultivated.

The mushroom flies were raised at room temperature using a plastic box (30 × 30 × 30 cm) filled with a sawdust medium cultivated with mushroom hyphae. Water was supplemented at intervals of one day to adjust moisture in the medium, The mushroom flies were transferred to a plastic box filled with the medium and used for the experiment while massively growing.

Experiments were carried out three times in the experiment of inserting 100 mushroom flies and inspecting dead individuals after 30 minutes, and it was deemed that a person with no movement was dead by using a fine brush to stimulate the body Respectively.

The insecticidal rate of the mushroom flies was examined by spraying the mixed extract of the insect repellent and the pine needle prepared in Example 1-1 with an atomizer. The mixed extract of Example 1-1-a was spray-dried by contacting the extract with a small amount of dry ice.

[Table 2]

As a result, the insecticidal rate of the experimental group sprayed with the composition of Example 1-1 in which the diluted solution of the insecticide extract of the present invention was diluted by 50 times and the diluted solution of the insecticidal extract by three times diluted was sprayed was 83% . On the other hand, in the experimental group of Comparative Example 1 in which the same amount of the insecticide was sprayed with 50 times diluted solution, the insecticidal effect was not sufficiently exhibited by recording the 31.3% insecticidal rate.

These results indicate that the insecticidal effect inherent to the insecticide bacterium is significantly increased by combining the insecticidal insecticidal insecticidal extract (see Comparative Example 2) with the insecticidal insecticide. As a result, the insecticidal effect of the insecticidal insecticidal insecticide I can see that it plays a role.

On the other hand, in the experimental group sprayed with the fogging method of Example 1-1-a, all the mushroom flies were observed after 30 minutes of spraying. Based on these results, it was found that the insecticidal effect of the pest- It was found to be more effective when sprayed in mist form as a carrier.

As a result, it was found that the pesticide composition of the present invention, which is mixed with a relatively low-cost pesticide, as a raw material for natural insecticide sold in the market, is economical, quick, and has excellent control effect.

2-1. Ant control

The insecticidal activity of the extract prepared in Example 1 was tested in the laboratory to determine the insecticidal rate against the ants.

The ants were placed in a plastic box (30 × 30 × 30 cm) and used for the experiment.

Experiments were conducted by repeating the experiment of injecting 100 ants adults and examining the dead individuals after 30 minutes, and measuring the dead body by stimulating the body using a fine brush.

The insecticidal rates of the ants were examined by spraying the mixed extracts of the pest control and the pest control prepared in Examples 1-2 and 1-3 with an atomizer. The mixed extract of Example 1-3-a was sprayed on a small amount of dry ice in contact with the extract.

[Table 3]

As a result, in the experimental group sprayed with the composition of Example 1-2 in which the diluted solution of the insecticide extract of the present invention was diluted 400 times and the diluted solution of the insecticidal extract was diluted 50 times, the insecticidal rate was 59.3% And the insecticidal rate was increased by a factor of two compared to the insecticidal rate of 33.7% in the experimental group of Comparative Example 5 sprayed with the same amount of the 400-fold diluent.

In the experimental group sprayed with the composition of Example 1-3 in which the diluted solution of the insecticidal extract of the present invention was diluted 400 times and the diluted solution of the diluted extract of the insecticidal extract by 25 times was sprayed, the average insecticidal rate was 87.7% , And the insecticidal effect was increased as the dilution ratio of bamboo extract was decreased.

From these results, it was observed that the insecticidal effect inherent in the bug bug was markedly increased as the bug bug extract (see example 6) with insecticidal effect was combined with the bug bug.

On the other hand, in the experimental group sprayed with the fogging method of Example 1-3-a, all the ants were controlled after 30 minutes from the spraying. Based on these results, the insecticidal effect of the pest- And it was found to be more effective when sprayed in mist form.

From the above results, it can be seen that the insecticide composition of the present invention has a quick and excellent controlling effect, and it can be effectively applied to prevent crop damage caused by insect pests and life insects I could confirm. Therefore, the insecticidal composition of the present invention can be effectively utilized as an effective environmentally friendly insecticide which replaces chemical pesticides used as insecticides for controlling and preventing insects.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention.

Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (6)

Containing an extract of Tanacetum cinerariifolium and Agastache rugosa as an active ingredient,
Characterized in that the said insect repellent ( Tanacetum cinerariifolium and Agastache rugosa ) are mixed in a weight ratio of 1: 10-30.
Pesticide composition. The method according to claim 1,
Wherein the extract is extracted with a solvent selected from the group consisting of water, a lower alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof. delete The method according to claim 1,
The composition may be any one selected from the group consisting of suspension, sustained-emulsion, wettable powder, granular concentrate, microemulsion, wettable powder, granule, powder, emulsion, spray, ≪ / RTI > The method according to claim 1,
Wherein the composition is a smoothing agent dispersed with a carbon dioxide gas as a carrier. Preparing an aqueous solution by mixing water and a composition of any one of claims 1, 2, 4, and 5;
Contacting the prepared aqueous solution with carbon dioxide gas sublimated in dry ice to produce a mixed gas; And
Treating the mixed gas to insect pests;
Lt; RTI ID = 0.0 > pest < / RTI >

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