KR101093011B1 - Composition for acaricide against and repellent to Tetranychus urticae, comprising sandalwood oil or ?, ?-santalol - Google Patents

Abstract

The present invention relates to a novel natural insecticide having excellent astringent effect, repellent effect and scattering inhibitory effect on spotted mite, and more particularly to a spotted mite acaricide or repellent composition comprising sandalwood oil as an active ingredient, α The present invention relates to a spotted marrow acaricide or repellent composition comprising a mixture of xantalol and β-santhalol as an active ingredient. Spotted mite acaricide or repellent composition according to the present invention has excellent astringent effect, repellent effect and scattering inhibitory effect on the spotted mite.

Sandalwood Oil, α, β-Santalol, Spot Mite, Acaricide, Repellent, Scattering Inhibitor

Description

Composition for acaricide against and repellent to Tetranychus urticae, comprising sandalwood oil or α, β-santalol}

The present invention relates to a novel natural insecticide having excellent killing effect, repelling effect and scattering inhibitory effect on spot mite.

Tetranychus urticae is an omnivorous pest that lives and harms all crops such as fruit trees, corn, and soybeans, and vegetables, flowers, weeds, common crops, and flowers. It occurs in many parts of the world, including Korea, Japan, the United States, and Europe, and the damage from plant cultivation crops is increasing due to its wide host range. It mainly lives on the back side of the leaves and sucks the juice. The damage leaves have yellow or white spots, and if the damage is severe, the whole leaves become yellowish brown and fall prematurely. Spotted mites are known to occur during the year in the house and over eight to ten generations a year. As the breeding speed increases, the density increases rapidly, and the damage rapidly develops.

Therefore, control agents for controlling such mites have been developed and used. Examples of acaricides used for controlling mites include kelof (dicofol), tedion: tetradifon, chlorofenson, ecarol (bromopropylate), Dozens of species such as chinomethionat, azocyclotin, dichron bifenthrin granule smoker, chias diphdpi smoker (powering) and phenprogranule smoker (danitol) are registered. Spotted mite has a high density in the lower lobe close to the branch, so care should be taken to spray enough liquid on the lower part of the plant.

On the other hand, the conventional control means for the mite is a lot of dependence on the drug, due to the high cost and side effects of the drug is a situation that places a lot of burden on the farm. In addition, the use of organic synthetic pesticides has greatly improved the productivity of agriculture, but due to its excessive use, the current situation is that the pests and resistance of secondary pests other than spotted mite are greatly developed and are suffering from the difficulties of controlling at the same time.

Particularly, in the control of mites, the developmental speed of the drug resistance of the mites is one of the great difficulties. The malignant resistance of the mites varies according to the types of acaricides, and even in the case of spotted mites, several organophosphorus pesticides such as parathion and organic A silkworm culling test with chlorine-based dipofol revealed significant differences in the resistance development of acaricides. On the other hand, acaricide-resistant mites are often pointed out as a big problem in carrying out chemical control because they often exhibit cross-resistance to other drugs not involved in culling, and excessive pesticides are used to chemically control resistant spotted mites. This causes problems such as destruction of agricultural ecosystems, environmental pollution, human toxicity, and the addition of drug-resistant pests.

Therefore, as a part of the solution to solve this problem, it is urgent to develop not only an excellent insecticide effect but also an environmentally friendly natural insecticide. In particular, the vegetable insecticide is attracting attention as one of the alternative pesticides as a biopesticide.

Accordingly, the inventors of the present invention, while trying to solve the problems of the prior art, while researching a natural insecticide for effectively controlling the spotted mite, sandalwood oil and α, β-santalol is spotted mite The present invention has been completed by finding that it exhibits excellent acaricide effect, repellent effect and scatter suppression effect.

It is therefore an object of the present invention to provide a spotted mite acaricide composition comprising sandalwood oil as an active ingredient.

Another object of the present invention is to provide a spotted mite acaricide composition comprising a mixture of α-anthalol and β-anthalol as an active ingredient.

Another object of the present invention is to provide a spotted mite repellent composition comprising sandalwood oil as an active ingredient.

Another object of the present invention is to provide a spotted mite repellent composition comprising a mixture of α-anthalol and β-anthalol as an active ingredient.

In order to achieve the object of the present invention as described above, the present invention provides a spotted mite acaricide or repellent composition comprising sandalwood oil as an active ingredient.

In one embodiment of the present invention, the sandalwood oil may contain α-santhalol and β-santhalol.

In one embodiment of the present invention, the sandalwood oil may be included 0.01 to 50% by weight relative to the total weight of the composition.

The present invention also provides a spotted mite acaricide or repellent composition comprising a mixture of α-anthalol and β-anthalol as an active ingredient.

In one embodiment of the present invention, the mixing ratio of the α-anthalol and β-anthalol may be 1: 1 to 3: 1.

In one embodiment of the present invention, the mixture of α-anthalol and β-anthalol may be included in an amount of 0.01 to 50 wt% based on the total weight of the composition.

Sandalwood oil or α, β-santhalol containing spotted mite acaricide or repellent composition according to the present invention has excellent astringent, repellent and scattering inhibitory effect on spotted mite, and because it uses an essential oil component extracted from natural plants There is no toxicity to humans or animals, and furthermore, it is an environmentally friendly effect that does not cause environmental pollution, and also has an effect of showing a very high spot control rate at low concentrations.

The present invention relates to a novel natural insecticide having excellent astringent effect, repellent effect and scattering inhibitory effect on spotted mite, and more particularly, to provide a spotted mite acaricide or repellent composition comprising sandalwood oil as an active ingredient, It is characterized by providing a spotted marrow acaricide or repellent composition comprising a mixture of α-anthalol and β-anthalol as an active ingredient.

In the present invention, the composition comprising the sandalwood oil (Sandalwood oil) of the present invention as an active ingredient or a composition comprising a mixture of α-santalol and β-santalol as an active ingredient Spotted mite ( Tetranychus urticae ) was found for the first time that there is an excellent fertilizing effect, repellent effect and spawning inhibitory effect.

Firstly, the present inventors extracted essential oil components from 34 kinds of plants to obtain insecticidal active substances against spotted mite from natural plants, and confirmed the insecticidal effect against spotted mite.

As a result, the present inventors confirmed that the commontime oil and sandalwood oil of the essential oils extracted from 34 kinds of plants have an acaricidal effect against spotted mites, and compared with the commontime oil. It was confirmed that the oil spraying effect is better.

In addition, according to an embodiment of the present invention, the common time oil in the essential oil extracted from 34 plants showed an insecticide rate of about 62% against spotted mites, and sandalwood oil about 89% against spotted mites It was confirmed that the insecticide rate of. In addition, sandalwood oil was found to significantly reduce the total number of scattering compared to other plant essential oils also have an inhibitory effect on scattering (see Example 2-1).

Accordingly, the present inventors have expected that sandalwood oil can be used for controlling spotted mites by confirming that sandalwood oil has an excellent killing effect and scattering inhibitory effect against spotted mites. Could.

On the other hand, in the present invention, sandalwood oil having excellent acaricide and scattering inhibitory effect against the spotted mite can be extracted and purified from the natural using extraction and purification methods conventional in the art, or by a chemical method Can be synthesized. Preferably the sandalwood oil can be extracted and purified from a natural plant called sandalwood (Sandalwood, Sandalwood).

The type of extraction and purification methods in the art, but is not limited thereto, one method selected from steam distillation method, hot water extraction method, solvent extraction method, compression method, carbon dioxide gas extraction method, reflux cooling extraction method, cold leaching method, elution method It can be done with

In one embodiment of the present invention, sandalwood oil was extracted by using a steam distillation method. Looking at the process, the sandalwood was first cut into 0.5 to 3 cm or shredded with a mixer, dried, and then put into a flask and steam. Distill In other words, about 10 times the amount of water in the flask containing the sample and extract the essential oil components while refluxing in an essential oil extraction apparatus for 2 to 5 hours at 90 ~ 120 ℃.

On the other hand, the present inventors have confirmed that sandalwood oil has a strong insecticidal and scattering inhibitory activity against spotted mite, the main component of sandalwood oil having this activity was analyzed using GC / MS.

As a result, the main components of sandalwood oil were identified as α-santhalol and β-santhalol, and they have a structure as shown in the following formulas (1) and (2).

"Santalol" is a material constituting the main component of the sandalwood oil is a compound having a chemical formula of C15H24O, molecular weight 220.36. Fractional distillation of sandalwood oil yields a colorless viscous liquid, a mixture of isomers of α and β species, with α-santholol being segorisquiterpene alcohols and β-santhalol being Igoresquiterpene alcohols to be.

In addition, the present inventors investigated whether the α-santhalol and β-santhalol, which are main components of sandalwood oil, have an insecticidal effect against spotted mites. It is preferable that the mixing ratio of alpha-anthalol and beta-anthalol of the present invention is 1: 1 to 3: 1. According to one embodiment of the present invention, it was found that the compound having a mixture of α-santhalol 46% and β-santhalol 20% has an acaricide effect of about 85% against spot mites. In addition, the number of scattering was also markedly reduced, indicating that there was also a scattering inhibitory effect (see Example 4).

Therefore, the mixture of α-anthalol and β-anthalol according to the present invention also has a pesticidal effect against spotted mites, like sandalwood oil.

Furthermore, the present inventors examined whether sandalwood oil and α-santhol and β-santhalol have an effect of avoiding spot mites, according to the present invention. It was confirmed that the mixture of and β-santhalol also has a good repellent effect on the spotted mite, and the effect of avoiding the spotted mite lasted up to 5 hours (see Examples 5 and 6).

Therefore, the present inventors confirmed that sandalwood oil and a mixture of α-santhalol and β-santalol showed strong insecticidal activity against spotted mites, as well as an excellent astringent effect and a repellent and scatter suppressing effect.

Therefore, the present invention provides a spotted mite acaricide or repellent composition comprising sandalwood oil as an active ingredient.

The present invention also provides a spotted mite acaricide or repellent composition comprising a mixture of α-anthalol and β-anthalol as an active ingredient.

The acaricide or repellent composition according to the present invention preferably comprises 0.01 to 50% by weight of sandalwood oil. In addition, the acaricide composition or repellent composition preferably contains 0.01 to 50% by weight of α-anthalol and β-anthalol in the form of a single or a mixture. However, the content of the plant essential oil or insecticidal compound of the present invention may be applied differently depending on the use of the pesticide, the formulation, and the method of spraying.

The "caricide" of the present invention refers to a drug that selectively kills mites. Acaricides should be effective against eggs, larvae, as well as eggs, and have long residual effectiveness. In addition, only mites should have a selective effect, be effective against several mites, and be free from crop harm.

In addition, the "repellant" refers to a drug that stimulates pests or small animals so that they do not come close.

The acaricide composition or repellent composition of the present invention can be used where the spot mites inhabit, and the method of use is preferably a coating treatment, dipping treatment, fumigation treatment, and the like. Can be used as

In addition, the acaricide composition or repellent composition of the present invention may be prepared and applied in various forms. It can be prepared in the form of a liquid, emulsion, coating or granules by dissolving or dispersing in one or more solvents for application and dipping treatment, and extenders such as liquid solvents, using surfactants commonly used for fumigation treatments, It is preferably used in combination with a pressurized liquefied gas or a solid carrier in the form of a smoker, a fumigant, a coating agent, a granule, or a solid agent.

To prepare such various forms of preparations, solvents and additives commonly used in the art can be used in the compositions according to the invention. For example, it can be prepared in liquid form using ethanol or water harmless to the human body as a dispersion.

In addition, the composition of the present invention may be prepared in solid form further comprising an excipient according to the method of use. The excipient uses a suitable material according to the use and application method of the composition, all materials included in conventional pesticides can be used.

In addition, the composition of the present invention may further include an aromatic component (fragrance) in order to remove the unpleasant odor and give a fresh aroma when using the composition in a residential space. The fragrance ingredient may be one selected from natural or chemically synthesized fragrances such as fruit and flower fragrances or a mixture of two or more thereof.

In addition, the composition of the present invention may further include a corrosion inhibitor selected from the group consisting of sodium nitrite, sodium benzoate and mixtures thereof, in order to inhibit corrosion of the metal container containing the composition.

Hereinafter, the present invention will be described in detail with reference to Examples and drawings. However, these examples are intended to illustrate the present invention in more detail, and the scope of the present invention is not limited to these examples.

< Example  1>

According to the invention Plant essential oils  extraction

The present inventors extracted essential oil components from 34 kinds of plants described below, essential oils (Essential oil), raw material (sorce plant), extraction sites, extraction methods extracted from each plant are shown in Table 1 below.

Raw Material, Extraction Site and Extraction Method of Plant Essential Oil Used in the Present Invention No Essential oil Raw material Extraction site Extraction Method One Ginger Rosemarinus off camphre Rhizome Water vapor distillation 2 Citronella Cymbopogon winteriani petal Water vapor distillation 3 Cinnamom Cinnamomum zelanicum fell Water vapor distillation 4 Cajeput tree Melaleuca leucodendron leaf Water vapor distillation 5 Geranium Pelargonium roseum Tip of flower and leaf Water vapor distillation 6 Commontime Thymus zygis thymol Flower Water vapor distillation 7 Scotch pine Pinus sylvestris Conifer Water vapor distillation 8 Sweet orange Citrus sinensis skin pressure 9 Cedar wood Cedrus atlantica tree Water vapor distillation 10 Blue Gum Eucalyptus globus leaf Water vapor distillation 11 Juniper Juniperus communis fruit Water vapor distillation 12 Clove bud syzigium aromaticum Eye Water vapor distillation 13 Sweet basil Occimum basilicum Flowers and leaves Water vapor distillation 14 Cypress Cupressus sempervirens Branch Water vapor distillation 15 Eucalyptus Eucalyptus radiata leaf Water vapor distillation 16 Clary sage Salvia sclarea leaf pressure 17 Lemon peel Citrus limon skin pressure 18 Ylang-Ylang Cananga odorata Flower Water vapor distillation 19 Tea-tree Melaleuca alternifolia leaf Water vapor distillation 20 Sandalwood Santalum austrocaledonicum tree Water vapor distillation 21 Bergamot Citrus aurantium ssp . skin pressure 22 Niaouli Melaleuca viridiflora leaf pressure 23 Grapefruit Citrus paradisi rose skin pressure 24 Black pepper Pipper nigrum Fruit Water vapor distillation 25 Lemongrass Cymbopogon citratus Leaf and stem Water vapor distillation 26 Sweet marjoram Origanum majorana Flowers and leaves Water vapor distillation 27 True lavender Lavandula Vera A flowered tip Water vapor distillation 28 Myrrh Commiphora myrrha Flowers and trees Water vapor distillation 29 Peppermint Mentha piperita leaf Water vapor distillation 30 Rosemary Rosemarinus off camphre Flowers and leaves Water vapor distillation 31 Bitter orange Citrus aurantium var armara Eye Water vapor distillation 32 Frankinsence Boswellia carterii sap Water vapor distillation 33 Hyssop Hyssopus officinnalis leaf Water vapor distillation 34 Patchouli Pogestemon cablin leaf Water vapor distillation

< Example  2>

Spotted Plant essential oil  Ingredient Acaricide effect

In order to confirm whether the 34 kinds of plant essential oils extracted in Example 1 have insecticidal activity against spotted mite, the following experiment was performed.

<2-1> 34 kinds Plant essential oils  Spotted Acaricide effect

To confirm the acaricide effect of 34 plant essential oils against spotted mites, the following experiments were conducted. A wet cotton ball was placed on a Petri dish, and rounded kidney bean leaf slices (2-5 cm in diameter) were placed on the wet cotton wool, and 30 adult females were inoculated. Thereafter, a plant essential oil solution such as A was prepared. A solution is a solution prepared by first emulsifying by mixing ethanol in each plant essential oil and adding an appropriate amount of Triton X-100 as a kind of surfactant. When this A solution was used for the experiment, 180 ml of water was added and diluted. In addition, solution B is a composition except for plant essential oil in solution A, and is used as a control, and C is not treated.

A: 200 μl each Essential oil + 20 mL ethanol + Triton X-100 + 180 mL water

B: Ethanol 20ml + Triton X-100 + 180ml Water

C: control (nothing handled)

The kidney bean leaf slices were soaked for 5 seconds in the solution prepared as described above, and insecticide and scattering water were measured after 24 hours. This experiment was repeated three times in total, and the experimental results are shown in Table 2 below. Statistical analysis was performed using the Tukey HSD test method.

Experimental Results on the Fertilizing Effect of 34 Plant Essential Oils Refined name
(Essential oil) Insecticide rate (%)
(Mortality) Total oviposition Oviposition per live adult ginger 14.7 ± 11.7 ab 242.0 ± 41.07 ab 10.2 ± 0.2 a Citronella 29.3 ± 22.5 ab 192.3 ± 48.01 ab 8.6 ± 1.7 a Cinnamon 25.2 ± 23.1 ab 211.0 ± 51.57 ab 10.8 ± 1.6 a Kazefoot Tree 25.4 ± 14.4 ab 201.3 ± 13.32 ab 9.5 ± 1.4 a geranium 31.6 ± 18.1 ab 193.3 ± 13.05 ab 10.6 ± 3.1 a Common time 62.8 ± 42.4 ab 131.7 ± 151.41 ab 17.3 ± 9.4 a Scotch Pine 51.4 ± 19.7 ab 159.7 ± 53.53 ab 14.6 ± 11.1 a Sweet Orange 46.3 ± 38.2 ab 123.0 ± 51.86 ab 9.4 ± 2.9 a Cedarwood 15.1 ± 5.0 ab 224.0 ± 63.00 ab 9.5 ± 3.2 a Blue sword 22.8 ± 17.4 ab 209.7 ± 50.85 ab 9.6 ± 1.9 a Juniper 43.7 ± 23.3 ab 128.0 ± 81.96 ab 7.9 ± 3.4 a Clove Bird 42.6 ± 37.1 ab 174.3 ± 118.32 ab 10.2 ± 0.9 a Sweet basil 23.8 ± 14.0 ab 209.3 ± 62.36 ab 10.5 ± 1.9 a cypress 30.4 ± 20.7 ab 198.7 ± 55.52 ab 10.7 ± 1.5 a Eucalyptus 30.0 ± 21.9 ab 201.0 ± 36.35 ab 9.9 ± 2.2 a Clary sage 9.8 ± 4.8 ab 214.7 ± 77.50 ab 8.7 ± 3.2 a Lemon peel 37.7 ± 34.7 ab 129.3 ± 68.65 ab 8.5 ± 1.7 a Ylang-ylang 26.6 ± 13.7 ab 221.7 ± 111.29 ab 10.2 ± 4.2 a Tea tree 31.1 ± 32.4 ab 142.7 ± 70.50 ab 7.7 ± 2.3 a Sandalwood 89.4 ± 8.6 a 28.0 ± 10.00 b 13.2 ± 5.2 a Bergamot 13.4 ± 10.1 b 225.0 ± 24.06 ab 10.2 ± 0.9 a Nyauli 29.3 ± 29.0 ab 213.0 ± 85.04 ab 10.8 ± 0.4 a Grapefruit 32.9 ± 34.2 ab 188.7 ± 70.81 ab 11.7 ± 3.6 a Black Pepper 24.8 ± 21.5 ab 239.0 ± 76.71 ab 12.0 ± 1.2 a Lemongrass 19.9 ± 12.4 ab 210.3 ± 48.43 ab 10.0 ± 4.0 a Sweet marjoram 9.8 ± 4.8 b 236.7 ± 18.58 ab 9.5 ± 0.5 a True Lavender 27.7 ± 23.4 ab 226.3 ± 85.66 ab 11.0 ± 1.3 a Mir 25.3 ± 16.5 ab 189.7 ± 70.19 ab 8.9 ± 2.0 a Peppermint 25.3 ± 20.9 ab 215.7 ± 52.29 ab 10.5 ± 1.8 a Rosemary 14.8 ± 19.0 ab 212.3 ± 33.29 ab 9.3 ± 2.3 a Bitter orange 23.0 ± 24.1 ab 205.3 ± 58.11 ab 10.1 ± 0.8 a Franken Sense 27.4 ± 26.0 ab 219.0 ± 98.32 ab 10.3 ± 2.4 a Hyssop 30.0 ± 23.3 ab 196.7 ± 55.77 ab 9.5 ± 1.7 a Patchouli 23.3 ± 15.6 ab 243.3 ± 86.03 ab 10.9 ± 1.6 a Ethanol + triton X + water (B) 13.6 ± 4.2 b 227.0 ± 27.07 ab 10.8 ± 1.3 a control (C) 2.9 ± 5.0 b 323.7 ± 51.96 a 13.1 ± 3.6 a
Statistical analysis result df = 35,72;
F = 1.46;
P = 0.0588 df = 35,72;
F = 1.56;
P = 0.0576 df = 35,72;
F = 0.89;
P = 0.6423

As a result of testing the fertilization effect of the 34 kinds of plant essential oils, as shown in Table 2, Commontime oil (Commonthyme oil) showed a pesticidal rate of about 62% against spotted mites, sandalwood oil ( Sandalwood oil) exhibited a pesticidal rate of about 89% for spotted mites. In addition, sandalwood oil was found to significantly reduce the total number of scattering, it was confirmed that there is also a scattering inhibitory effect.

<2-2> Two kinds of insecticides with more than 60% Plant essential oils  Insecticide

The experiment was carried out in the same manner as in <2-1> with respect to common-time oil (Commonthyme oil) and sandalwood oil (Sandalwood oil) showing a pesticidal rate of 60% or more in the <2-1> experiment. The results are shown in Table 3 below.

Experimental Results of the Fertilization Effect on Common-Time Oil and Sandalwood Oil Essential oil Insecticide (%) (Mortality) Total oviposition Sandalwood 87.2 ± 2.9a 1.3 ± 0.6b Common time 5.7 ± 4.0b 8.0 ± 1.6a Ethanol + triton X + water (B) 1.1 ± 2.0b 8.2 ± 2.5ab control (C) 1.1 ± 2.0b 10.5 ± 2.2a
Statistical analysis result df = 3,8;
F = 109.62;
P = <. 0001 df = 3,8;
F = 11.74;
P = 0.0027

As a result, as shown in Table 3, the fertilization effect of the common time oil was about 5.7%, while the sandalwood oil showed about 90% of the fertilizing effect, and the scattering inhibitory effect, the common time oil Showed a total scatter of about 228, while sandalwood oil had a total scatter of about 33.3.

Therefore, the present inventors were able to confirm that sandalwood oil has an excellent acaricide effect and scattering inhibitory effect against spotted mite. Therefore, the inventors could expect that sandalwood oil could be used for controlling spotted mites.

< Example  3>

Sandalwood  oil( Sandalwood oil Analysis of main ingredients of

The major components of sandalwood oil were analyzed by selecting sandalwood oil, which showed the highest insecticidal rate in Example 2. GC / MS 2010 was used as an analyzer, and the analysis conditions were as follows.

Injection temperature (split 1:10): 220 ° C

Oven 40 ~ 200 ℃ at 2 ℃ / min

Detection temperature (FID): 250 ° C

Carrier gas: Helium

Flow rate: 1 ml / min

Source temperature: 180 ° C

DB-WAX column (J & W scientific): 30 mm x 0.25 mm, I.d. x 0.25 micron

The results of component analysis under the above conditions are shown in FIG. 1.

As a result, the main components of the sandalwood oil through the GC / MS analyzer was shown to be α-santhol and β-santalol represented by the following formula (1) and (2).

[Formula 1]

[Formula 2]

< Example  4>

α, β- Of anthalol (α, β-santalol)  Spotted Acaricide effect

In order to confirm the acaricide effect on the spotted mite of α, β-santhalol (α, β-santalol), which is a main component of the sandalwood oil extracted from the present invention, the following experiment was conducted. First, a mixed solution of 46% α-anthalol and 20% β-anthalol was prepared, and then the experiment was conducted in the same manner as in Example 1 for spotted mites. The experimental results are shown in Table 4 below. Indicated.

Experimental results of acaricide effect of α, β-santhalol Refined name
(Essential oil) Insecticide rate (%)
(Mortality) Total oviposition Oviposition per live adult α, β-santhalol 85.5 ± 2.9 a 18.3 ± 3.5 c 4.6 ± 0.3 c Ethanol + triton X + water (B) 0.0 ± 0.0 b 213.3 ± 10.1 b 8.7 ± 0.6 b control (C) 0.0 ± 0.0 b 348.0 ± 16.1 a 14.2 ± 1.0 a
Statistical analysis result df = 2, 6;
F = 9121.20;
P = <. 0001 df = 2, 6;
F = 661.71;
P = <. 0001 df = 2, 6;
F = 149.35;
P = <. 0001

As a result, as shown in Table 4, it was confirmed that the α, β-santhalol compound, which is a major component of sandalwood, showed about 85% acaricide effect against spotted mites. Therefore, it was found that the α, β-santhalol compound, like sandalwood oil, has an excellent acaricide and scatter suppressing effect against spotted mites.

< Example  5>

Spotted Plant essential oil  Avoidance effect of ingredients

In order to confirm whether the 34 kinds of plant essential oils extracted in Example 1 has a repellent effect against spotted mite, the following experiment was performed.

The experiment was divided into (A) selection test and (B) no-choice test, and the experimental schematic is shown in FIG. 3. Twenty-four hours after plant essential oil treatment, (1) T1 and U1 move by walking, (2) T2 and U2 move remaining numbers, and (3) T3 and U3 throw up and drop threads, (4) C1 represents the number of birds on the top of the stem, and finally the eggs were examined.

<5-1> 34 kinds Plant essential oils  Avoidance effect on spotted mites

In order to confirm the repellent effect of 34 kinds of plant essential oils on spotted mite.

(A) Selection test ( choice test )

Two leaves of kidney beans were designated as treated and untreated leaves one by one, and the treatment group was prepared by mixing 200 μl of one kind of essence oil and 20 ml of ethanol and a solution of Triton X-100 and 180 ml of water. After soaking in the solution for 10 seconds and drying, the pressure sensitive adhesive was treated on the top of the center stem. Thereafter, 30 females of the spotted mite were inoculated into the central stem and the change was checked 24 hours later.

The results of spotted M. larvae and spawning avoidance (per female) effect according to this experiment are shown in Table 5 below, and the analysis results for the total number of eggs are shown in Table 6 below. For statistical processing, t-test was used to compare the two treatments.

Experimental results of adult attraction and spawning avoidance (per female) Refined name
Attraction of spotted mites Spotted Mite female spawning
process
No treatment P value
(t-test)
process
No treatment P value
(t-test) ginger 19.7 ± 3.1 9.3 ± 3.2 0.014 13 ± 4.3 11 ± 4.1 0.539 Citronella 12.0 ± 7.0 17.7 ± 7.6 0.395 14 ± 1.3 11 ± 1.7 0.116 Cinnamon 10.7 ± 3.5 21.3 ± 4.5 0.032 13 ± 3.6 12 ± 3.1 0.636 Kazefoot Tree 13.3 ± 4.0 17.0 ± 3.5 0.324 12 ± 4.5 13 ± 2.8 0.766 geranium 14.3 ± 5.0 14.7 ± 0.6 0.919 16 ± 6.1 12 ± 4.1 0.353 Common time 6.3 ± 4.2 24.3 ± 3.1 0.004 13 ± 4.5 16 ± 4.7 0.369 Scotch Pine 8.7 ± 2.5 20.0 ± 5.3 0.029 15 ± 0.4 15 ± 1.8 0.519 Sweet Orange 11.0 ± 4.0 16.0 ± 4.6 0.228 13 ± 4.2 16 ± 0.9 0.330 Cedarwood 10.7 ± 7.2 23.7 ± 5.0 0.063 14 ± 4.4 14 ± 4.0 0.850 Blue sword 16.7 ± 3.2 14.7 ± 4.5 0.566 13 ± 2.1 13 ± 3.6 0.795 Juniper 5.7 ± 4.0 20.3 ± 7.5 0.025 21 ± 17.5 11 ± 3.7 0.405 Clove Bird 8.7 ± 3.1 22.7 ± 2.3 0.004 14 ± 3.8 13 ± 1.5 0.627 Sweet basil 6.7 ± 5.5 24.7 ± 7.0 0.025 14 ± 3.6 15 ± 0.3 0.882 cypress 10.3 ± 8.5 19.7 ± 7.0 0.217 14 ± 5.3 13 ± 4.1 0.489 Eucalyptus 12.7 ± 5.9 17.7 ± 7.6 0.376 10 ± 6.2 14 ± 1.8 0.345 Clary sage 9.7 ± 3.1 20.3 ± 2.5 0.010 15 ± 2.9 15 ± 1.4 1,000 Lemon peel 12.3 ± 8.6 19.7 ± 7.5 0.377 14 ± 1.5 15 ± 1.0 0.275 Ylang-ylang 4.7 ± 3.8 23.7 ± 3.8 0.004 11 ± 3.7 15 ± 1.4 0.138 Tea tree 8.3 ± 3.2 20.7 ± 1.2 0.003 16 ± 4.2 16 ± 0.5 1,000 Sandalwood 3.3 ± 3.5 26.0 ± 4.0 0.003 9 ± 8.1 12 ± 3.6 0.511 Bergamot 9.3 ± 0.6 22.0 ± 3.5 0.029 16 ± 3.8 11 ± 4.0 0.197 Nyauli 7.0 ± 3.5 20.7 ± 5.5 0.026 13 ± 5.7 16 ± 3.4 0.593 Grapefruit 8.0 ± 7.0 24.3 ± 7.2 0.048 12 ± 6.8 14 ± 8.5 0.736 Black Pepper 5.7 ± 5.5 21.3 ± 2.5 0.007 15 ± 5.7 13 ± 7.9 0.737 Lemongrass 5.7 ± 5.5 13.7 ± 1.5 0.577 11 ± 6.0 13 ± 3.7 0.827 Sweet marjoram 9.7 ± 7.5 21.7 ± 6.1 0.098 14 ± 4.4 16 ± 2.4 0.060 True Lavender 8.0 ± 1.0 21.7 ± 4.7 0.008 15 ± 2.8 15 ± 2.5 0.652 Mir 8.0 ± 2.0 20.7 ± 1.5 0.001 12 ± 8.6 17 ± 3.2 0.314 Peppermint 9.7 ± 4.5 17.0 ± 6.1 0.169 15 ± 4.5 14 ± 1.8 0.658 Rosemary 9.0 ± 6.6 20.0 ± 6.0 0.099 16 ± 2.7 16 ± 1.3 0.729 Bitter orange 6.3 ± 4.2 24.0 ± 4.4 0.007 17 ± 4.8 15 ± 2.7 0.553 Franken Sense 8.7 ± 3.1 19.0 ± 3.5 0.018 18 ± 2.8 15 ± 3.7 0.353 Hyssop 8.7 ± 3.8 19.7 ± 5.8 0.018 12 ± 5.7 14 ± 0.2 0.657 Patchouli 9.0 ± 3.6 20.7 ± 3.1 0.013 18 ± 1.3 15 ± 1.4 0.039

Effect Test on Total Scattering Count (choice test) Refined name
T. urticae eggs on leaf process No treatment P value (t-test) ginger 256.3 ± 65.4 113.0 ± 81.7 0.0766 Citronella 171.3 ± 116.4 185.7 ± 68.3 0.8630 Cinnamon 146.7 ± 76.6 251.0 ± 76.3 0.1699 Kazefoot Tree 161.7 ± 66.2 232.3 ± 86.9 0.3251 geranium 217.3 ± 28.4 175.0 ± 64.1 0.3546 Common time 94.3 ± 91.9 397.0 ± 110.8 0.0219 Scotch Pine 133.0 ± 38.3 293.7 ± 89.7 0.0462 Sweet Orange 139.3 ± 43.1 256.0 ± 66.1 0.0626 Cedarwood 155.0 ± 133.8 327.7 ± 39.2 0.0985 Blue sword 213.0 ± 71.4 187.0 ± 69.1 0.6739 Juniper 70.7 ± 26.1 235.0 ± 124.1 0.0882 Clove Bird 127.0 ± 72.0 292.3 ± 53.6 0.0332 Sweet basil 106.3 ± 96.0 368.5 ± 109.2 0.0385 cypress 157.0 ± 162.0 218.7 ± 30.3 0.9449 Eucalyptus 133.7 ± 111.7 254.0 ± 94.1 0.2771 Clary sage 152.0 ± 76.5 226.3 ± 65.4 0.0563 Lemon peel 166.7 ± 124.0 294.0 ± 126.0 0.2802 Ylang-ylang 57.0 ± 57.5 358.0 ± 88.7 0.0079 Tea tree 123.0 ± 27.5 330.7 ± 8.1 0.0002 Sandalwood 38.3 ± 34.5 323.0 ± 113.9 0.0144 Bergamot 147.3 ± 45.8 244.3 ± 101.3 0.2053 Nyauli 92.7 ± 66.3 313.0 ± 30.0 0.0063 Grapefruit 92.7 ± 76.0 295.3 ± 74.6 0.0301 Black Pepper 84.3 ± 74.9 293.0 ± 191.0 0.1529 Lemongrass 210.3 ± 175.6 150.7 ± 49.4 0.6013 Sweet marjoram 110.0 ± 119.1 374.7 ± 60.9 0.0266 True Lavender 121.7 ± 23.0 315.0 ± 84.3 0.0186 Mir 82.7 ± 56.6 358.7 ± 46.9 0.0029 Peppermint 134.7 ± 30.9 245.0 ± 109.8 0.1691 Rosemary 131.7 ± 89.2 329.7 ± 122.5 0.0864 Bitter orange 101.3 ± 51.8 355.7 ± 122.3 0.0295 Franken Sense 157.0 ± 75.5 291.7 ± 113.4 0.1620 Hyssop 105.3 ± 66.1 269.7 ± 82.0 0.0540 Patchouli 158.3 ± 52.4 314.3 ± 66.4 0.0331

As a result, as shown in Table 5, among the 34 plant essential oils, 20 kinds of plant essential oils, namely, Cinnamom, Commontime, Scotch pine and Juniper , Clove bud, Sweet basil, Clari sage, Ylang-Ylang, Tea-tree, Sandalwood, Bergamot, Nia Niaouli, Grapefruit, Black pepper, True lavender, Myrrh, Bitter orange, Frankin sence, Hyssop, Patcholy (Patchouly) was found to have a repelling effect on the adult in spotted mite.

In addition, as shown in Table 6, 14 kinds of plant essential oils for the total scattered water, that is, Commontime (Commonthyme), Scotch pine (Ccotch pine), Clove bud, Sweet basil (Sweet basil) ), Ylang-Ylang, Tea-tree, Sandalwood, Niaouli, Grapefruit, Sweet marjoram, True lavender, Myrrh, Bitter orange (Patchouly) was found to have a scattering avoidance effect.

<5-2> 20 kinds of repellent effects Plant essential oils Non-selective  Test

In the selection test of Example <5-1>, 20 kinds of plant essential oils whose repellent effects were recognized were subjected to a non-selection test.

(B) Non-selective  Test( No - choice test )

Of the two main leaves of the kidney bean, two leaves were designated as treatment, and the other one was removed. The treatment was immersed and dried for 10 seconds in a preparation solution containing a mixture of 200 μl of one kind of essence oil and 20 ml of ethanol, a solution of Triton X-100 and 180 ml of water, and then treated with an adhesive on the top of the center stem. It was. Thereafter, 30 females were spotted on the central stem and the change was checked after 24 hours, and the experimental results are shown in Table 7 below. Statistical analysis was performed using the Tukey HSD test method.

Experimental results of adult attraction and spawning avoidance (per female)
Refined name Adult attraction of spotted mite 30 minutes 1h 2h 3h 5h 12h 24h Scattering Water (total) Cinnamon 28.0 ± 1.7a 28.7 ± 1.5b 29.0 ± 1.0a 29.7 ± 0.6a 29.7 ± 0.6a 29.7 ± 0.6a 29.7 ± 0.6a 238.3 ± 25.0ab Common time 24.0 ± 2.6a 25.7 ± 3.2b 27.0 ± 3.6a 28.3 ± 1.5a 29.0 ± 1.0a 29.3 ± 1.2a 29.3 ± 1.2a 233.7 ± 45.7ab Scotch Pine 28.3 ± 2.9a 29.7 ± 0.6b 29.7 ± 0.6a 29.7 ± 0.6a 30.0 ± 0.0a 30.0 ± 0.0a 30.0 ± 0.0a 241.3 ± 21.1ab Juniper 22.7 ± 7.6a 24.3 ± 4.7b 27.7 ± 3.2a 28.0 ± 2.6a 28.3 ± 2.1a 29.0 ± 1.0a 29.7 ± 0.6a 245.0 ± 41.9ab Clove Bird 24.3 ± 2.5a 25.3 ± 1.5b 27.0 ± 1.0a 27.3 ± 1.5a 27.7 ± 1.5a 28.3 ± 1.2a 28.7 ± 1.5a 214.0 ± 75.1ab Sweet basil 23.7 ± 6.5a 27.0 ± 3.0b 27.3 ± 2.3a 29.0 ± 1.0a 29.3 ± 1.2a 29.3 ± 1.2a 29.3 ± 1.2a 251.0 ± 18.1ab Clary sage 27.3 ± 2.5a 27.3 ± 2.5b 29.3 ± 0.6a 29.7 ± 0.6a 29.7 ± 0.6a 29.7 ± 0.6a 30.0 ± 0.0a 222.3 ± 53.8ab Ylang-ylang 26.0 ± 4.0a 27.0 ± 4.4b 28.3 ± 2.9a 29.0 ± 1.7a 29.3 ± 1.2a 30.0 ± 0.0a 30.0 ± 0.0a 236.3 ± 10.4ab Tea tree 24.3 ± 4.0a 24.7 ± 4.5b 26.7 ± 3.1a 27.3 ± 2.5a 27.7 ± 2.1a 27.7 ± 2.1a 27.7 ± 2.1a 197.7 ± 42.1ab Sandalwood 1.0 ± 1.0b 2.3 ± 3.2b 6.3 ± 2.5b 12.3 ± 1.2b 16.0 ± 5.0b 26.3 ± 3.2a 30.0 ± 0.0a 145.0 ± 24.6b Bergamot 27.0 ± 2.6a 28.7 ± 2.3b 29.7 ± 0.6a 29.7 ± 0.6a 29.7 ± 0.6a 29.7 ± 0.6a 29.7 ± 0.6a 242.3 ± 28.7ab Nyauli 23.0 ± 6.2a 23.0 ± 6.2b 27.3 ± 1.2a 28.0 ± 1.7a 29.3 ± 0.6a 29.7 ± 0.6a 30.0 ± 0.0a 192.3 ± 77.5ab Grapefruit 25.0 ± 4.6a 26.0 ± 3.0b 27.7 ± 2.3a 29.0 ± 1.a 29.3 ± 0.6a 29.3 ± 0.6a 29.7 ± 0.6a 270.3 ± 18.8a Black Pepper 22.0 ± 2.0a 23.3 ± 4.2b 27.3 ± 2.9a 29.0 ± 1.0a 29.3 ± 0.6a 29.3 ± 0.6a 29.3 ± 0.6a 205.0 ± 31.0ab True Lavender 27.3 ± 2.5a 28.0 ± 2.6b 29.3 ± 0.6a 29.7 ± 0.6a 29.7 ± 0.6a 29.7 ± 0.6a 29.7 ± 0.6a 214.7 ± 18.1ab Mir 16.3 ± 1.5a 17.0 ± 2.6b 26.0 ± 1.7a 28.0 ± 1.7a 28.0 ± 1.7a 29.0 ± 1.7a 29.0 ± 1.7a 215.0 ± 24.6ab Bitter orange 25.0 ± 6.1a 25.3 ± 6.4b 27.7 ± 3.2a 28.3 ± 2.9a 28.3 ± 2.9a 28.3 ± 2.9a 28.3 ± 2.9a 196.0 ± 55.3ab Franken Sense 25.0 ± 7.8a 29.0 ± 1.0b 29.7 ± 0.6a 30.0 ± 0.0a 30.0 ± 0.0a 30.0 ± 0.0a 30.0 ± 0.0a 209.0 ± 34.8ab Hyssop 22.0 ± 7.5a 23.3 ± 7.6b 25.3 ± 7.2a 27.0 ± 5.2a 28.3 ± 2.9a 28.7 ± 2.3a 28.7 ± 2.3a 212.7 ± 42.1ab Patchouli 16.3 ± 2.3a 19.7 ± 8.1b 25.0 ± 6.1a 27.3 ± 3.8a 28.3 ± 2.9a 28.3 ± 2.9a 28.3 ± 2.9a 226.7 ± 34.9ab control 29.3 ± 0.6a 29.7 ± 0.6b 30.0 ± 0.0a 30.0 ± 0.0a 30.0 ± 0.0a 30.0 ± 0.0a 30.0 ± 0.0a 239.7 ± 11.6ab Statistical analysis result df = 20,42
F = 5,82 P = <. 0001 df = 20,42
F = 6.45 P = <. 0001 df = 20,42
F = 8.74 P = <. 0001 df = 20,42
F = 10.43 P = <. 0001 df = 20,42
F = 7.82 P = <. 0001 df = 20,42
F = 1.12 P = 0.3652 df = 20,42
F = 0.81 P = 0,6832 df = 20,42
F = 1.37 P = 0,1929

As a result, as shown in Table 7, among the 20 kinds of plant essential oils in which the repellent effect was recognized in the selective test, sandalwood oil against spotted mites at 30 minutes, 1 hour, 2 hours, 3 hours and 5 hours It was found that the repellent effect appeared, and the duration of the repellent effect was also maintained for 5 hours.

< Example  6>

α, β- Of anthalol (α, β-santalol)  Avoidance effect on spotted mites

The present inventors confirm that the sandalwood oil has an excellent repelling effect against spotted mite through the results of Example 5, and thus the effect of avoiding spotted mite on α, β-santalol which is a major component of sandalwood oil. It was. To this end, the experiment was conducted in the same manner as in Example 5 with a mixed solution of 46% α-anthalol and 20% β-anthalol, and the experimental results are shown in Tables 8, 9, and 10 below. Indicated. For statistical processing, t-test was used to compare the two treatments.

Adult test and effect of avoiding laying eggs (per female) (choice test)
Composition
Attraction of spotted mites Spotted Mite female spawning process No treatment P value
(t-test) process No treatment P value
(t-test) α, β-santhalol 2.0 ± 1.0 27.7 ± 0.6 <0.0001 1 ± 1.7 11 ± 1.1 0.0015

Effect Test on Total Scattering Count (choice test) Composition
T. urticae eggs on leaf process No treatment P value (t-test) α, β-santhalol 1.0 ± 1.7 314.0 ± 31.7 <0.0001

Test results of adult attraction and spawning avoidance (per female) (No-choice test)
Composition Adult attraction of spotted mite 30 minutes 1h 2h 3h 5h 12h 24h Spawning water
(all) α, β-santhalol 0.0 ± 0.0b 0.7 ± 1.2b 1.3 ± 1.2b 6.7 ± 1.5b 17.0 ± 1.0b 30.0 ± 0.0a 30.0 ± 0.0a 251.3 ± 38.9a Ethanol + Triton-X_Water 24.0 ± 0.0a 27.3 ± 1.5b 28.0 ± 1.0a 30.0 ± 0.0a 30.0 ± 0.0a 30.0 ± 0.0a 30.0 ± 0.0a 287.7 ± 60.1a control 27.3 ± 2.3a 30.0 ± 0.0a 30.0 ± 0.0a 30.0 ± 0.0a 30.0 ± 0.0a 30.0 ± 0.0a 30.0 ± 0.0a 337.3 ± 19.6a Statistical analysis result df = 2, 6
F = 281.57
P = <. 0001 df = 2, 6
F = 645.82
P = <. 0001 df = 2, 6
F = 988.0;
P = <. 0001 df = 2, 6
F = 700.0;
P = <. 0001 df = 2, 6
F = 507.0;
P = <. 0001
-
- df = 2, 6
F = 3.04
P = 0.1223

-: Statistical processing was not possible because the values were the same in all iterations of all treatments (ie, there was no variation between iterations) (eg 30.0 ± 0.0).

As a result, as shown in Table 8 and Table 9, it was shown that α, β-santalol had a repelling effect and scattering inhibitory effect against spotted mites, as shown in Table 10, α, β -It was found that xantalol had a repelling effect on spotted mites at 30 minutes, 1 hour, 2 hours, 3 hours, and 5 hours, and the duration of the repelling effect was also maintained at 5 hours.

Therefore, the present inventors were able to confirm that the sandalwood oil extracted from the plant has excellent killing effect, repelling effect and scattering suppression effect against spotted mite. In addition, it was confirmed that α-santhalol and β-santhalol, which are the main components of sandalwood oil, also have excellent acaricide, repellent and scatter suppression effects against spotted mites.

So far I looked at the center of the preferred embodiment for the present invention. Those skilled in the art will appreciate that the present invention can be implemented in a modified form without departing from the essential features of the present invention. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope will be construed as being included in the present invention.

1 shows a graph of GC / MS results of the sandalwood oil according to the present invention, peak 1 is α-santalol, peak 2 is β-santalol, and peak 3 is acid Talol (Santalol) is shown.

Figure 2 shows a graph of the GC / MS results of the santrol composition standard used as a control agent in the present invention, the peak 1 is α-santhalol (α-santalol), the peak 2 is β-santalol (β-santalol) It is shown.

3 is a diagram showing an experimental schematic of a selection test according to the present invention.

4 is a diagram showing an experimental schematic of a non-selective test according to the present invention.

Claims (4)

Spotted mite acaricide or repellent composition comprising sandalwood oil as an active ingredient. The spotted mite acaricide or repellent composition according to claim 1, wherein the sandalwood oil contains α-anthalol and β-anthalol. Spotted mite acaricide or repellent composition comprising a mixture of α-anthalol and β-anthalol as an active ingredient. According to claim 3, wherein the mixing ratio of the α-anthalol and β-anthalol is a weight ratio of 1: 1 to 3: 1 spotted mite acaricide or repellent composition, characterized in that.

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